Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effect of different levels of dietary sweet orange (citrus sinensis) peel extract on humoral immune system responses in broiler chickens. | this experiment was conducted to evaluate the effects of different levels of sweet orange (citrus sinensis) peel extract (sope) on humoral immune system responses in broiler chickens. three hundred 1-day broilers (ross-308) were randomly allocated to treatments varying in supplemental sope added in the drinking water. the experimental groups consisted of three treatments fed for 42 days as follows: a control treatment without feed extract, a treatment containing 1000 ppm of sope and a treatment ... | 2015 | 24990585 |
| high incidence of preharvest colonization of huanglongbing-symptomatic citrus sinensis fruit by lasiodiplodia theobromae (diplodia natalensis) and exacerbation of postharvest fruit decay by that fungus. | huanglongbing (hlb), presumably caused by the bacterium "candidatus liberibacter asiaticus," is a devastating citrus disease associated with excessive preharvest fruit drop. lasiodiplodia theobromae (diplodia) is the causal organism of citrus stem end rot (ser). the pathogen infects citrus fruit under the calyx abscission zone (az-c) and is associated with cell wall hydrolytic enzymes similar to plant enzymes involved in abscission. by means of dna sequencing, diplodia was found in "ca. liberiba ... | 2015 | 25344245 |
| activity of six essential oils extracted from tunisian plants against legionella pneumophila. | the aim of this study was to investigate the composition of six essential oils extracted from tunisian plants, i.e., artemisia herba-alba asso, citrus sinensis (l.) osbeck, juniperus phoenicea l., rosmarinus officinalis l., ruta graveolens l., and thymus vulgaris l., and to evaluate their activity against legionella pneumophila (microdilution assays). eight legionella pneumophila strains were studied, including the two well-known serogroup 1 lens and paris strains as controls and six environment ... | 2015 | 26460561 |
| pharmacological evidence for the folk use of nefang: antipyretic, anti-inflammatory and antinociceptive activities of its constituent plants. | nefang is a polyherbal anti-malarial composed of mangifera indica ( mib and mil; bark and leaf), psidium guajava ( pg ), carica papaya ( cp ), cymbopogon citratus ( cc ), citrus sinensis ( cs ) and ocimum gratissimum ( og ) (leaves). previous studies have demonstrated its in vitro and in vivo antiplasmodial activities, antioxidant properties and safety profile. this study aimed at evaluating the antipyretic, anti-inflammatory and antinociceptive activities of the constituent plants of nefang whi ... | 2015 | 26055261 |
| the efficacy and underlying mechanism of sulfone derivatives containing 1,3,4-oxadiazole on citrus canker. | the objectives of the current study were to isolate and identify the pathogen responsible for citrus canker and investigate the efficacy of sulfone derivatives containing 1,3,4-oxadiazole moiety on controlling citrus canker caused by xanthomonas citri subsp. citri (xcc) under in vitro and field conditions. in an in vitro study, we tested eight sulfone derivatives against xcc and the results demonstrated that compound 3 exhibited the best antibacterial activity against xcc, with a half-maximal ef ... | 2015 | 26247929 |
| accumulation of transcription factors and cell signaling-related proteins in the nucleus during citrus-xanthomonas interaction. | the nucleus is the maestro of the cell and is involved in the modulation of cell signaling during stress. we performed a comprehensive nuclear proteome analysis of citrus sinensis during interaction with host (xanthomonas citri pv. citri-xcc) and non-host (xanthomonas oryzae pv. oryzae-xoo) pathogens. the nuclear proteome was obtained using a sequential method of organelle enrichment and determined by nano-lc-ms/ms analysis. a total of 243 proteins accumulated differentially during citrus-xantho ... | 2015 | 26202445 |
| an environmentally safe larvicide against aedes aegypti based on in situ gelling nanostructured surfactant systems containing an essential oil. | liquid crystalline precursors, which are in situ gelling nanostructured surfactant systems, can undergo phase transition in aqueous solution and become more structured aggregates, controlling release of larvicides and acting as biotechnology alternatives for dengue control. such systems can contain bioactive substances as citrus sinensis essential oil (cseo) which exhibits biological activity against aedes aegypti (ae. aegypti) larvae. | 2015 | 26125515 |
| quantification and localization of hesperidin and rutin in citrus sinensis grafted on c. limonia after xylella fastidiosa infection by hplc-uv and maldi imaging mass spectrometry. | a high performance liquid chromatography-ultraviolet (hplc-uv) method was developed for quantifying hesperidin and rutin levels in leaves and stems of citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg ml(-1) respectively, with r(2)>0.999 for all curves. the limits of detection (lod) for both flavonoids were 0.6 and 0.5 μg ml(-1), respectively, with quantification (loq) being 2.0 and 1.0 μg ml(-1), respectively. the quantification method was applied to citrus sinensis ... | 2015 | 25749617 |
| fungal planet description sheets: 400-468. | novel species of fungi described in the present study include the following from australia: vermiculariopsiella eucalypti, mulderomyces natalis (incl. mulderomyces gen. nov.), fusicladium paraamoenum, neotrimmatostroma paraexcentricum, and pseudophloeospora eucalyptorum on leaves of eucalyptus spp., anungitea grevilleae (on leaves of grevillea sp.), pyrenochaeta acaciae (on leaves of acacia sp.), and brunneocarpos banksiae (incl. brunneocarpos gen. nov.) on cones of banksia attenuata. novel foli ... | 2016 | 27616795 |
| an apple plus a brazil nut a day keeps the doctors away: antioxidant capacity of foods and their health benefits. | antioxidant-rich foods scavenge free radicals and other reactive species, decreasing the risk of different non-communicable chronic diseases. the objective of this study was to review the content of total antioxidant capacity of commonly foods comparing with experimental data and to explore the health benefits due to foods with moderate to high tac. the tac was analytically measured using the "total antioxidant capacity" (nx2332) test from randox® (uk) by spectrometry at 600 nm. brazil nut (bert ... | 2016 | 26572874 |
| effect of essential oils on meat and fat qualities of crossbred young bulls finished in feedlots. | twenty-seven animals (½ angus - ½ nellore) were fed for four months with one of the following diets: without addition of essential oils (e0.0), with 3.5 (e3.5) or 7 (e7.0) g/animal/day of an essential oil blend (oregano, garlic, lemon, rosemary, thyme, eucalyptus and sweet orange). chemical composition, fatty acid profile and meat color were evaluated in longissimus muscle. in addition, the effects of aging (one, seven and 14days) on the meat water holding capacity, texture and lipid oxidation w ... | 2016 | 27388818 |
| larvicidal effects of four citrus peel essential oils against the arbovirus vector aedes albopictus (diptera: culicidae). | in this study, we evaluated the larvicidal activity of four citrus essential oils (eos; sweet orange, mandarin, bergamot, and lemon) against the arbovirus vector aedes albopictus (skuse) (diptera: culicidae) under laboratory conditions. through gas chromatography-mass spectrometry (gc-ms) analyses, we found that in sweet orange, mandarin, and lemon eos, limonene was the most abundant compound, whereas linalyl acetate was the most abundant in the bergamot eo. all tested eos showed a marked larvic ... | 2016 | 26357845 |
| citrus functional genomics and molecular modeling in relation to citrus sinensis (sweet orange) infection with xylella fastidiosa (citrus variegated chlorosis). | citrus are among the economically most important fruit tree crops in the world. citrus variegated chlorosis (cvc), caused by xylella fastidiosa infection, is a serious disease limiting citrus production at a global scale. with availability of citrus genomic resources, it is now possible to compare citrus expressed sequence tag (est) data sets and identify single-nucleotide polymorphisms (snps) within and among different citrus cultivars that can be exploited for citrus resistance to infections, ... | 2016 | 27447755 |
| rapid and accurate identification of xanthomonas citri subspecies citri by fluorescence in situ hybridization. | citrus canker is an economically important disease caused by the bacterial pathogen xanthomonas citri subsp. citri (xcc). this organism targets a wide range of citrus plants, including sweet orange, grapefruit, lemon and lime. as xcc is spread by environmental factors such as wind and rain, it is difficult to control its movement once the disease has established. in order to facilitate monitoring of citrus canker we sought to design a novel diagnostic protocol based on fluorescence in situ hybri ... | 2016 | 27492577 |
| an engineered promoter driving expression of a microbial avirulence gene confers recognition of tal effectors and reduces growth of diverse xanthomonas strains in citrus. | xanthomonas citri ssp. citri (x. citri), causal agent of citrus canker, uses transcription activator-like effectors (tales) as major pathogenicity factors. tales, which are delivered into plant cells through the type iii secretion system (t3ss), interact with effector binding elements (ebes) in host genomes to activate the expression of downstream susceptibility genes to promote disease. predictably, tales bind ebes in host promoters via known combinations of tale amino acids to dna bases, known ... | 2016 | 27362693 |
| modification of the ptha4 effector binding elements in type i cslob1 promoter using cas9/sgrna to produce transgenic duncan grapefruit alleviating xccδptha4:dcslob1.3 infection. | citrus canker caused by xanthomonas citri subspecies citri (xcc) is a severe disease for most commercial citrus cultivars and responsible for significant economic losses worldwide. generating canker-resistant citrus varieties will provide an efficient and sustainable solution to control citrus canker. here, we report our progress in generating canker-resistant grapefruit by modifying the ptha4 effector binding elements (ebes) in the cslob1 promoter (ebeptha4 -cslobp) of the cslob1 (citrus sinens ... | 2016 | 27071672 |
| temporal transcription profiling of sweet orange in response to ptha4-mediated xanthomonas citri subsp. citri infection. | citrus canker, caused by xanthomonas citri subsp. citri, is a devastating disease of most commercial citrus varieties. in our previous study, we analyzed the transcriptional response of 'valencia' sweet orange to x. citri subsp. citri wild-type and ptha4 mutant infection at 48 h postinoculation (hpi). using microarray analysis, two ptha4 targets, cslob1 and cssweet1, were identified. we have shown that ptha4 binds to the effector binding element (ebe) of cslob1 and activates gene expression of t ... | 2016 | 26780431 |
| additive roles of pthas in bacterial growth and pathogenicity associated with nucleotide polymorphisms in effector-binding elements of citrus canker susceptibility genes. | citrus canker, caused by xanthomonas citri, affects most commercial citrus varieties. all x. citri strains possess at least one transcription activator-like effector of the ptha family that activates host disease susceptibility (s) genes. the x. citri strain 306 encodes four ptha effectors; nevertheless, only ptha4 is known to elicit cankers on citrus. as none of the pthas act as avirulence factors on citrus, we hypothesized that pthas 1-3 might also contribute to pathogenicity on certain hosts. ... | 2016 | 26709719 |
| reduced susceptibility to xanthomonas citri in transgenic citrus expressing the fls2 receptor from nicotiana benthamiana. | overexpression of plant pattern-recognition receptors by genetic engineering provides a novel approach to enhance plant immunity and broad-spectrum disease resistance. citrus canker disease associated with xanthomonas citri is one of the most important diseases damaging citrus production worldwide. in this study, we cloned the fls2 gene from nicotiana benthamiana cdna and inserted it into the binary vector pbinplus/ars to transform hamlin sweet orange and carrizo citrange. transgene presence was ... | 2016 | 26554734 |
| major components of orange oil inhibit staphylococcus aureus growth and biofilm formation, and alter its virulence factors. | bovine mastitis is a costly disease in the dairy industry and does not always respond to antibiotic treatment. the major components of terpeneless, cold-pressed valencia orange oil - citral, linalool, decanal and valencene - were examined as potential alternative treatments for staphylococcus aureus-associated mastitis. the minimum inhibitory concentration (mic) of all four components against s. aureus was determined after incubation for 24 h. growth inhibition assays were performed for all effe ... | 2016 | 27259704 |
| potent inhibition of pseudogymnoascus destructans, the causative agent of white-nose syndrome in bats, by cold-pressed, terpeneless, valencia orange oil. | the causative agent of white-nose syndrome (wns), pseudogymnoascus destructans, has been shown to be fatal to several species of bats in north america. to date, no compounds or chemical control measures have been developed which eliminates the growth of the fungus in the environment or in affected animals. in the current study, we evaluated the activity of cold-pressed, terpeneless orange oil (cpt) against multiple isolates of p. destructans in vitro. for all assays, a modified kirby-bauer disk ... | 2016 | 26849057 |
| involvement of an ethylene response factor in chlorophyll degradation during citrus fruit degreening. | chlorophyll degradation naturally occurs during plant senescence. however, in fruit such as citrus, it is a positive characteristic, as degreening is an important colour development contributing to fruit quality. in the present work, citrus sinensis osbeck, cv. newhall fruit was used as a model for chlorophyll degradation. an ethylene response factor, citerf13, was isolated and its transcriptional changes were closely correlated with fruit peel degreening during development or in response to eth ... | 2016 | 27037684 |
| substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast. | flavonoids, which comprise a large family of secondary plant metabolites, have received increased attention in recent years due to their wide range of features beneficial to human health. one of the most abundant flavonoid skeletons in citrus species is the flavanone naringenin, which is accumulated as glycosides containing terminal rhamnose (rha) after serial glycosylation steps. the linkage type of rha residues is a determining factor in the bitterness of the citrus fruit. such rha residues ar ... | 2016 | 26433966 |
| antimicrobial effects of citrus sinensis peel extracts against dental caries bacteria: an in vitro study. | ethnomedicine is gaining admiration since years but still there is abundant medicinal flora which is unrevealed through research. the study was conducted to assess the in vitro antimicrobial potential and also determine the minimum inhibitory concentration (mic) of citrus sinensis peel extracts with a view of searching a novel extract as a remedy for dental caries pathogens. | 2016 | 26855710 |
| high temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant pichia kudriavzevii kvmp10. | this work explores the potential for the development of orange peel based ethanol bioprocesses through isolation of the thermotolerant pichia kudriavzevii kvmp10. a model solution of hydrolysed valencia orange peel was employed to determine the ethanologenic potential of the yeast, which was maximized at 42°c producing 54 g l(-1) of ethanol. the effect of orange peel oil on bioethanol formation was investigated at 30 and 42°c confirming that the minimum inhibitory peel oil content was 0·01% (v/v ... | 2016 | 26510181 |
| comparative transcriptional profiling of orange fruit in response to the biocontrol yeast kloeckera apiculata and its active compounds. | the yeast kloeckera apiculata strain 34-9 is an antagonist that shows biological control activity against the postharvest fungal pathogens of citrus. an antifungal compound, 2-phenylethanol (pea), has been identified from the extract of k. apiculata. to better understand the molecular processes underlying the response of citrus fruit tissue to k. apiculata, the extract and pea, microarray analyses were performed on navel oranges using an affymetrix citrus genechip. | 2016 | 26725242 |
| antimycotic activity and genotoxic evaluation of citrus sinensis and citrus latifolia essential oils. | the aim of this study was to evaluate the antifungal activity of essential oils (eos) of citrus sinensis (c. sinensis) and citrus latifolia (c. latifolia) against five candida species: candida albicans, candida tropicalis, candida glabrata, candida lusitaniae and candida guilliermondii; and perform its genotoxic evaluation. the eos of c. sinensis and c. latifolia were obtained from the peel by hydro-distillation. the major components determined by gc-ms were in c. sinensis, d-limonene (96%) and ... | 2016 | 27137128 |
| citrus leprosis virus c infection results in hypersensitive-like response, suppression of the ja/et plant defense pathway and promotion of the colonization of its mite vector. | leprosis is a serious disease of citrus caused by citrus leprosis virus c (cilv-c, genus cilevirus) whose transmission is mediated by false spider mites of the genus brevipalpus. cilv-c infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. to get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of arabidopsis thaliana and ... | 2016 | 27933078 |
| citrus psorosis virus 24k protein interacts with citrus mirna precursors, affects their processing and subsequent mirna accumulation and target expression. | sweet orange (citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. here, we show that the infection of sweet orange plants with two isolates of citrus psorosis virus (cpsv) expressing different symptomatology alters the accumulation of a set of endogenous micrornas (mirnas). within these mirnas, mir156, mir167 and mir171 were the most down-regulated, with almost a three-fold re ... | 2016 | 26033697 |
| inactivation of plant-pathogenic fungus colletotrichum acutatum with natural plant-produced photosensitizers under solar radiation. | the increasing tolerance to currently used fungicides and the need for environmentally friendly antimicrobial approaches have stimulated the development of novel strategies to control plant-pathogenic fungi such as antimicrobial phototreatment (apt). we investigated the in vitro apt of the plant-pathogenic fungus colletotrichum acutatum with furocoumarins and coumarins and solar radiation. the compounds used were: furocoumarins 8-methoxypsoralen (8-mop) and 5,8-dimethoxypsoralen (isopimpinellin) ... | 2016 | 27434699 |
| ultrastructural changes in the epidermis of petals of the sweet orange infected by colletotrichum acutatum. | postbloom fruit drop (pfd) is an important disease caused by the fungus colletotrichum acutatum. pfd is characterised by the formation of necrotic lesions on the petals and stigmas of flowers as well as premature abscission of the fruit in citrus spp. we compare the ultrastructure of the epidermis of uninoculated citrus sinensis petals with that of petals inoculated with the fungus to understand the changes that occur upon c. acutatum infection. healthy petals have a cuticle with parallel striat ... | 2016 | 26334287 |
| comparison of the composition and antimicrobial activities of the essential oils of green branches and leaves of egyptian navel orange (citrus sinensis (l.) osbeck var. malesy). | the essential oils isolated from the leaves and green branches of the egyptian navel orange trees were analyzed by gc and gc/ms. a total of 33 and 24 compounds were identified from the oils of the leaves and branches accounting for 96.0% and 97.9%, respectively, of the total detected constituents. the major ones were sabinene (36.5; 33.0%), terpinen-4-ol (8.2; 6.2%), δ-3-carene (7.0; 9.4%), limonene (6.8; 18.7%), trans-ocimene (6.7; 6.1%), and β-myrcene (4.5; 4.4%). the antimicrobial activities ... | 2016 | 26948682 |
| transcriptome analysis of sweet orange trees infected with 'candidatus liberibacter asiaticus' and two strains of citrus tristeza virus. | huanglongbing (hlb) and tristeza, are diseases of citrus caused by a member of the α-proteobacteria, 'candidatus liberibacter asiaticus' (calas), and citrus tristeza virus (ctv) respectively. hlb is a devastating disease, but ctv strains vary from very severe to very mild. both calas and ctv are phloem-restricted. the calas-b232 strain and ctv-b6 cause a wide range of severe and similar symptoms. the mild strain ctv-b2 doesn't induce significant symptoms or damage to plants. | 2016 | 27169471 |
| anti-proliferative or cytopathic potential of thapsia garganica, citrus sinensis, citrus limon and vinca rosea extracts against human embryonic kidney carcinoma cell line. | developing anti-cancer drugs from natural products is receiving increasing interest worldwide due to limitations and side-effects of anti-cancer drugs. the purpose of this study was to explore the anti-proliferative or cytopathic potential of natural compounds derived from plant sources as alternatives of synthetic compounds on human embryonic kidney carcinoma (hek) cell line. | 2016 | 27618522 |
| the in vitro effect of selected essential oils on the growth and mycotoxin production of aspergillus species. | the aim of the present study was to assess the antifungal and anti-toxinogenic activity of 15 essential oils (eos) against three fungi of the genus aspergillus (a. parasiticus kmi-227-lr, a. parasiticus kmi-220-lr and a. flavus kmi-202-lr). the minimum inhibitory doses (mids) of the tested essential oils and their antifungal activity were determined using the micro-atmosphere method. the original commercial essential oil samples of jasminum officinale l., thymus vulgaris l., syzygium aromaticum ... | 2016 | 27322876 |
| analysis of whole chloroplast genomes from the genera of the clauseneae, the curry tribe (rutaceae, citrus family). | the clauseneae (aurantioideae, rutaceae) is a tribe in the citrus family that, although economically important as it contains the culinary and medicinally-useful curry tree (bergera koenigii), has been relatively understudied. due to the recent significant taxonomic changes made to this tribe, a closer inspection of the genetic relationships among its genera has been warranted. whole genome skimming was used to generate chloroplast genomes from six species, representing each of the four genera ( ... | 2016 | 27965082 |
| genome wide selection in citrus breeding. | genome wide selection (gws) is essential for the genetic improvement of perennial species such as citrus because of its ability to increase gain per unit time and to enable the efficient selection of characteristics with low heritability. this study assessed gws efficiency in a population of citrus and compared it with selection based on phenotypic data. a total of 180 individual trees from a cross between pera sweet orange (citrus sinensis osbeck) and murcott tangor (citrus sinensis osbeck x ci ... | 2016 | 27813590 |
| composition by lc-ms/ms of new carotenoid esters in mango and citrus. | interest in the composition of carotenoid esters of fruits is growing because esterification may affect their bioavailability. thus, the aim was to provide a detailed identification of carotenoid esters in citrus and mango. orange cv. 'valencia' and cv. 'pera' presented 9 free carotenoids, 38 monoesters, and 60 diesters. violaxanthin and luteoxanthin derivatives were the major ones, followed by antheraxanthin, lutein, zeaxanthin, β-cryptoxanthin, and zeinoxanthin esters, many of them reported fo ... | 2016 | 27712060 |
| coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings. | volatile organic compounds (vocs) are off-gassed from all living organisms and represent end products of metabolic pathways within the system. in agricultural systems, these vocs can provide important information on plant health and can ordinarily be measured non-invasively without harvesting tissue from the plants. previously we reported a portable gas chromatography/differential mobility spectrometry (gc/dms) system that could distinguish voc profiles of pathogen-infected citrus from healthy t ... | 2016 | 26695246 |
| changes in volatile and non-volatile flavor chemicals of "valencia" orange juice over the harvest seasons. | florida "valencia" oranges have a wide harvest window, covering four months after first reaching the commercial maturity. however, the influence of harvest time on juice flavor chemicals is not well documented, with the exception of sugars and acids. therefore, we investigated the major flavor chemicals, volatile (aroma), non-volatile (taste) and mouth feel attributes, in the two harvest seasons (march to june in 2007 and february to may in 2012). bitter limonoid compounds, limonin and nomilin, ... | 2016 | 28231099 |
| hybrid origins of citrus varieties inferred from dna marker analysis of nuclear and organelle genomes. | most indigenous citrus varieties are assumed to be natural hybrids, but their parentage has so far been determined in only a few cases because of their wide genetic diversity and the low transferability of dna markers. here we infer the parentage of indigenous citrus varieties using simple sequence repeat and indel markers developed from various citrus genome sequence resources. parentage tests with 122 known hybrids using the selected dna markers certify their transferability among those hybrid ... | 2016 | 27902727 |
| vascular epiphytes and host trees of ant-gardens in an anthropic landscape in southeastern mexico. | ant-gardens (ags) are considered one of the most complex mutualist systems between ants and plants, since interactions involving dispersal, protection, and nutrition occur simultaneously in them; however, little is known about the effects of the transformation of ecosystems on their diversity and interactions. in five environments with different land use within an anthropic landscape in southeastern mexico, we investigated the diversity and composition of epiphytes and host trees of ags built by ... | 2016 | 27866229 |
| draft genome sequence of curtobacterium sp. strain er1/6, an endophytic strain isolated from citrus sinensis with potential to be used as a biocontrol agent. | herein, we report a draft genome sequence of the endophytic curtobacterium sp. strain er1/6, isolated from a surface-sterilized citrus sinensis branch, and it presented the capability to control phytopathogens. functional annotation of the ~3.4-mb genome revealed 3,100 protein-coding genes, with many products related to known ecological and biotechnological aspects of this bacterium. | 2016 | 27856581 |
| ars-media for excel: a spreadsheet tool for calculating media recipes based on ion-specific constraints. | ars-media for excel is an ion solution calculator that uses "microsoft excel" to generate recipes of salts for complex ion mixtures specified by the user. generating salt combinations (recipes) that result in pre-specified target ion values is a linear programming problem. excel's solver add-on solves the linear programming equation to generate a recipe. calculating a mixture of salts to generate exact solutions of complex ionic mixtures is required for at least 2 types of problems- 1) formulati ... | 2016 | 27812202 |
| metabolomic comparative analysis of the phloem sap of curry leaf tree (bergera koenegii), orange jasmine (murraya paniculata), and valencia sweet orange (citrus sinensis) supports their differential responses to huanglongbing. | orange jasmine, murraya paniculata and curry leaf tree, bergera koenegii are alternative hosts for diaphorina citri, the vector of candidatus liberibacter asiaticus (clas), the pathogen of huanglongbing (hlb) in citrus. d. citri feeds on the phloem sap where clas grows. it has been shown that orange jasmine was a better host than curry leaf tree to d. citri. in addition, clas can infect orange jasmine but not curry leaf tree. here, we compared the phloem sap composition of these 2 plants to the ... | 2016 | 27763819 |
| protective effects of sweet orange, unshiu mikan, and mini tomato juice powders on t-bhp-induced oxidative stress in hepg2 cells. | the aim of this study was to investigate the protective effects of juice powders from sweet orange [citrus sinensis (l.) osbeck], unshiu mikan (citrus unshiu marcow), and mini tomato (solanum lycopersicum l.), and their major flavonoids, hesperidin, narirutin, and rutin in tert-butyl hydroperoxide (t-bhp)-induced oxidative stress in hepg2 cells. the increased reactive oxygen species and decreased glutathione levels observed in t-bhp-treated hepg2 cells were ameliorated by pretreatment with juice ... | 2016 | 27752497 |
| comparative analysis of mirnas and their target transcripts between a spontaneous late-ripening sweet orange mutant and its wild-type using small rna and degradome sequencing. | fruit ripening in citrus is not well-understood at the molecular level. knowledge of the regulatory mechanism of citrus fruit ripening at the post-transcriptional level in particular is lacking. here, we comparatively analyzed the mirnas and their target genes in a spontaneous late-ripening mutant, "fengwan" sweet orange (mt) (citrus sinensis l. osbeck), and its wild-type counterpart ("fengjie 72-1," wt). using high-throughput sequencing of small rnas and rna degradome tags, we identified 107 kn ... | 2016 | 27708662 |
| comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (citrus sinensis). | citrate, the predominant organic acid in citrus, determines the taste of these fruits. however, little is known about the synergic molecular processes regulating citrate accumulation. using 'dahongtiancheng' (citrus sinensis) and 'bingtangcheng' (c. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'dahongtiancheng' and 'bingtangcheng' exhibit significantly different patterns in ci ... | 2016 | 27507765 |
| identification and transcript profiles of citrus growth-regulating factor genes involved in the regulation of leaf and fruit development. | growth-regulating factor (grf) is an important protein in ga-mediated response, with key roles in plant growth and development. however, it is not known whether or how the grf proteins in citrus to regulate organ size. in this study, nine citrus grf genes (csgrf1-9) were validated from the 'anliu' sweet orange (al, citrus sinensis cv. anliu) by pcr amplification. they all contain two conserved motifs (qlq and wrc) and have 3-4 exons. the transcript levels of genes were detected by qrt-pcr. trans ... | 2016 | 27491940 |
| evaluation of the spatiotemporal dynamics of oxytetracycline and its control effect against citrus huanglongbing via trunk injection. | citrus huanglongbing (hlb) or greening is a devastating bacterial disease that has destroyed millions of trees and is associated with phloem-residing 'candidatus liberibacter asiaticus' (las) in florida. in this study, we evaluated the spatiotemporal dynamics of oxytetracycline in planta and its control effect against hlb via trunk injection. las-infected 'hamlin' sweet orange trees on 'swingle' citrumelo rootstock at the early stage of decline were treated with oxytetracycline hydrochloride (ot ... | 2016 | 27482624 |
| rapid discrimination of bergamot essential oil by paper spray mass spectrometry and chemometric analysis. | a novel approach for the rapid discrimination of bergamot essential oil from other citrus fruits oils is presented. the method was developed using paper spray mass spectrometry (ps-ms) allowing for a rapid molecular profiling coupled with a statistic tool for a precise and reliable discrimination between the bergamot complex matrix and other similar matrices, commonly used for its reconstitution. ambient mass spectrometry possesses the ability to record mass spectra of ordinary samples, in their ... | 2016 | 27460885 |
| aluminum toxicity-induced alterations of leaf proteome in two citrus species differing in aluminum tolerance. | seedlings of aluminum-tolerant 'xuegan' (citrus sinensis) and al-intolerant 'sour pummelo' (citrus grandis) were fertigated for 18 weeks with nutrient solution containing 0 and 1.2 mm alcl₃·6h₂o. al toxicity-induced inhibition of photosynthesis and the decrease of total soluble protein only occurred in c. grandis leaves, demonstrating that c. sinensis had higher al tolerance than c. grandis. using isobaric tags for relative and absolute quantification (itraq), we obtained more al toxicity-respon ... | 2016 | 27455238 |
| enhanced acquisition rates of 'candidatus liberibacter asiaticus' by the asian citrus psyllid (hemiptera: liviidae) in the presence of vegetative flush growth in citrus. | the asian citrus psyllid preferentially feeds and exclusively reproduces on young, newly emerged flush shoots of citrus. asian citrus psyllid nymphs feed and complete their life stages on these flush shoots. recent studies conducted under greenhouse conditions have shown that the transmission rates of 'candidatus liberibacter asiaticus' (clas), the putative causal agent of huanglongbing disease of citrus, are enhanced when flush shoots are present. however, it is unclear if clas acquisition by m ... | 2016 | 27451998 |
| long-term boron-excess-induced alterations of gene profiles in roots of two citrus species differing in boron-tolerance revealed by cdna-aflp. | boron (b) toxicity is observed in some citrus orchards in china. however, limited data are available on the molecular mechanisms of citrus b-toxicity and b-tolerance. using cdna-aflp, we identified 20 up- and 52 down-regulated genes, and 44 up- and 66 down-regulated genes from excess b-treated citrus sinensis and citrus grandis roots, respectively, thereby demonstrating that gene expression profiles were more affected in the latter. in addition, phosphorus and total soluble protein concentration ... | 2016 | 27446128 |
| repellent activity of botanical oils against asian citrus psyllid, diaphorina citri (hemiptera: liviidae). | the asian citrus psyllid, diaphorina citri kuwayama, is the insect vector of the pathogen causing huanglongbing. we selected three botanical oils to evaluate behavioral activity against d. citri. in laboratory olfactometer assays, fir oil was repellent to d. citri females, while litsea and citronella oils elicited no response from d. citri females. in choice settling experiments, d. citri settled almost completely on control plants rather than on plants treated with fir oil at a 9.5 mg/day relea ... | 2016 | 27429006 |
| systematic analysis of o-methyltransferase gene family and identification of potential members involved in the formation of o-methylated flavonoids in citrus. | the o-methylation of various secondary metabolites is mainly catalyzed by s-adenosyl-l-methionine (sam)-dependent o-methyltransferase (omt) proteins that are encoded by the o-methyltransferase gene family. citrus fruits are a rich source of o-methylated flavonoids that have a broad spectrum of biological activities, including anti-inflammatory, anticarcinogenic, and antiatherogenic properties. however, little is known about this gene family and its members that are involved in the o-methylation ... | 2016 | 26407870 |
| distribution of 'candidatus liberibacter asiaticus' above and below ground in texas citrus. | detection of 'candidatus liberibacter asiaticus' represents one of the most difficult, yet critical, steps of controlling huanglongbing disease. efficient detection relies on understanding the underlying distribution of bacteria within trees. to that end, we studied the distribution of 'ca. l. asiaticus' in leaves of 'rio red' grapefruit trees and in roots of 'valencia' sweet orange trees grafted onto sour orange rootstock. we performed two sets of leaf collection on grapefruit trees; the first ... | 2016 | 27050571 |
| principal component analysis (pca) of volatile terpene compounds dataset emitted by genetically modified sweet orange fruits and juices in which a d-limonene synthase was either up- or down-regulated vs. empty vector controls. | we have categorized the dataset from content and emission of terpene volatiles of peel and juice in both navelina and pineapple sweet orange cultivars in which d-limonene was either up- (s), down-regulated (as) or non-altered (ev; control) ("impact of d-limonene synthase up- or down-regulation on sweet orange fruit and juice odor perception"(a. rodríguez, j.e. peris, a. redondo, t. shimada, e. costell, i. carbonell, c. rojas, l. peña, (2016)) [1]). data from volatile identification and quantific ... | 2016 | 27689126 |
| nucleotides, micro- and macro-nutrients, limonoids, flavonoids, and hydroxycinnamates composition in the phloem sap of sweet orange. | currently, the global citrus production is declining due to the spread of huanglongbing (hlb). hlb, otherwise known as citrus greening, is caused by candidatus liberibacter asiaticus (clas) and is transmitted by the asian citrus psyllids (acp), diaphorina citri kuwayama. acp transmits clas bacterium while feeding on the citrus phloem sap. multiplication of clas in the phloem of citrus indicates that the sap contains all the essential nutrients needed for clas. in this study, we investigated the ... | 2016 | 27171979 |
| larvicidal potential of essential oils against musca domestica and anopheles stephensi. | the larvicidal activity of mentha piperita, cymbopogan citratus (lemongrass), eucalyptus globulus and citrus sinensis (orange) essential oils and their combinations was evaluated against musca domestica (housefly) and anopheles stephensi (mosquitoes) through contact toxicity assay. among all the tested essential oils/combinations, me. piperita was found to be the most effective larvicidal agent against mu. domestica and an. stephensi with lc50 values of 0.66 μl/cm(2) and 44.66 ppm, respectively, ... | 2016 | 26920567 |
| raman spectroscopy an option for the early detection of citrus huanglongbing. | this research describes the application of portable field raman spectroscopy combined with a statistical analysis of the resulting spectra, employing principal component analysis (pca) and linear discriminant analysis (lda), in which we determine that this method provides a high degree of reliability in the early detection of huanglongbing (hlb) on sweet orange, disease caused by the bacteria candidatus liberibacter asiaticus. symptomatic and asymptomatic plant samples of sweet orange (citrus si ... | 2016 | 26988658 |
| lrr-rlk family from two citrus species: genome-wide identification and evolutionary aspects. | leucine-rich repeat receptor-like kinases (lrr-rlks) represent the largest subfamily of plant rlks. the functions of most lrr-rlks have remained undiscovered, and a few that have been experimentally characterized have been shown to have important roles in growth and development as well as in defense responses. although rlk subfamilies have been previously studied in many plants, no comprehensive study has been performed on this gene family in citrus species, which have high economic importance a ... | 2016 | 27515968 |
| the wrky transcription factor family in citrus: valuable and useful candidate genes for citrus breeding. | wrky transcription factors belong to a large family of plant transcriptional regulators whose members have been reported to be involved in a wide range of biological roles including plant development, adaptation to environmental constraints and response to several diseases. however, little or poor information is available about wrky's in citrus. the recent release of completely assembled genomes sequences of citrus sinensis and citrus clementina and the availability of ests sequences from other ... | 2016 | 27193354 |
| the diversity of citrus endophytic bacteria and their interactions with xylella fastidiosa and host plants. | the bacterium xylella fastidiosa is the causal agent of citrus variegated chlorosis (cvc) and has been associated with important losses in commercial orchards of all sweet orange [citrus sinensis (l.)] cultivars. the development of this disease depends on the environmental conditions, including the endophytic microbial community associated with the host plant. previous studies have shown that x. fastidiosa interacts with the endophytic community in xylem vessels as well as in the insect vector, ... | 2016 | 27727362 |
| effect of benzyladenine on recovery of cryopreserved shoot tips of grapevine and citrus cultured in vitro. | the effect of n6-benzyladenine (ba) on the recovery of cryopreserved shoot tips of the ln33 hybrid (vitis l.) and troyer citrange [poncirus trifoliata (l.) raf. x citrus sinensis [l.] osbeck.] cultured in vitro was examined. for the ln33 hybrid, the presence of ba in the recovery medium was essential for survival of control and cryopreserved shoot tips, although the ba concentration did not influence the survival percentage. ba at 5, 2, and 5 microm or higher induced callus formation in control, ... | 2016 | 14566389 |
| the effect of gender and ethnicity on children's attitudes and preferences for essential oils: a pilot study. | aromatherapy is frequently recommended for children but children's preferences for specific essential oils are not well documented. | 2016 | 17681258 |
| olfactory response of anastrepha striata (diptera: tephritidae) to guava and sweet orange volatiles. | the behavioral responses of virgin and mated female anastrepha striata schiner (diptera: tephritidae) to guava (psidium guajava l.) or sweet orange (citrus sinensis l.) were evaluated separately using multilure traps in two-choice tests in field cages. the results showed that flies were more attracted to guava and sweet orange volatiles than to control (unbaited trap). the physiological state (virgin or mated) of females did not affect their attraction to the fruit volatiles. combined analysis o ... | 2016 | 25800723 |
| metabolic changes in citrus leaf volatiles in response to environmental stress. | citrus plants are well known as a rich source of vocs, and several have important roles in defense responses. however, how vocs are regulated in response to environmental stress is not yet well understood. in this study, we investigated dynamic changes of vocs present in leaves of seven citrus species (citrus sinensis, c. limon, c. paradisi, c. unshiu, c. kinokuni, c. grandis, and c. hassaku) in response to mechanical wounding, jasmonic acid (ja), and salicylic acid (sa) as determined by gas chr ... | 2016 | 26188419 |
| an rna-seq-based reference transcriptome for citrus. | previous rna-seq studies in citrus have been focused on physiological processes relevant to fruit quality and productivity of the major species, especially sweet orange. less attention has been paid to vegetative or reproductive tissues, while most citrus species have never been analysed. in this work, we characterized the transcriptome of vegetative and reproductive tissues from 12 citrus species from all main phylogenetic groups. our aims were to acquire a complete view of the citrus transcrip ... | 2016 | 26261026 |
| composition of citrus sinensis (l.) osbeck cv «maltaise demi-sanguine» juice. a comparison between organic and conventional farming. | juices from conventionally and organically grown citrus sinensis (l.) osbeck cv. maltaise demi-sanguine blood orange were investigated for quality parameters and antioxidant capacity. this blood orange variety is particularly rich in linoleic, linolenic acids, vitamin c and phenolic compounds. the quantitative determination of these compounds in cv. maltaise demi-sanguine juice produced under conventional and organic agricultural practices revealed significant differences. the organically grown ... | 2016 | 26471557 |
| field evaluation of plant defense inducers for the control of citrus huanglongbing. | huanglongbing (hlb) is currently the most economically devastating disease of citrus worldwide and no established cure is available. defense inducing compounds are able to induce plant resistance effective against various pathogens. in this study the effects of various chemical inducers on hlb diseased citrus were evaluated in four groves (three with sweet orange and one with mandarin) in florida (united states) for two to four consecutive growing seasons. results have demonstrated that plant de ... | 2016 | 26390185 |
| c- and o-glycosyl flavonoids in sanguinello and tarocco blood orange (citrus sinensis (l.) osbeck) juice: identification and influence on antioxidant properties and acetylcholinesterase activity. | sanguinello and tarocco are the blood orange (citrus sinensis (l.) osbeck) cultivars most diffused worldwide. reversed phase liquid chromatography coupled with ms-ms analysis showed that these two varieties have a similar chromatographic pattern, characterised by the presence of c- and o-glycosyl flavonoids. of the two, sanguinello was found to be far richer in flavonoids than tarocco. in the juices, twelve individual components were identified for the first time, namely, four c-glycosyl flavone ... | 2016 | 26593535 |
| regulation of cuticle formation during fruit development and ripening in 'newhall' navel orange (citrus sinensis osbeck) revealed by transcriptomic and metabolomic profiling. | fruit cuticle, which is composed of cutin and wax and biosynthesized during fruit development, plays important roles in the prevention of water loss and the resistance to pathogen infection during fruit development and postharvest storage. however, the key factors and mechanisms regarding the cuticle biosynthesis in citrus fruits are still unclear. here, fruit cuticle of 'newhall' navel orange (citrus sinensis osbeck) was studied from the stage of fruit expansion to postharvest storage from the ... | 2016 | 26795158 |
| spatio-temporal distribution and natural variation of metabolites in citrus fruits. | to study the natural variation and spatio-temporal accumulation of citrus metabolites, liquid chromatography tandem mass spectrometry (lc-ms) based metabolome analysis was performed on four fruit tissues (flavedo, albedo, segment membrane and juice sacs) and different citrus species (lemon, pummelo and grapefruit, sweet orange and mandarin). using a non-targeted metabolomics approach, more than 2000 metabolite signals were detected, from which more than 54 metabolites, including amino acids, fla ... | 2016 | 26775938 |
| thiamethoxam and imidacloprid drench applications on sweet orange nursery trees disrupt the feeding and settling behaviour of diaphorina citri (hemiptera: liviidae). | chemical control is the method most used for management of diaphorina citri, the vector of the phloem-limited bacteria associated with citrus huanglongbing (hlb) disease. the objectives of this study were to determine the influence of soil-drench applications of neonicotinoids (thiamethoxam and imidacloprid) on the probing behaviour of d. citri on citrus nursery trees, using the electrical penetration graph (epg) technique, and to measure the d. citri settling behaviour after probing on citrus n ... | 2016 | 26694803 |
| genome-wide identification of the transcription factors involved in citrus fruit ripening from the transcriptomes of a late-ripening sweet orange mutant and its wild type. | fruit ripening is a genetically programmed process. transcription factors (tfs) play key roles in plant development and ripening by temporarily and spatially regulating the transcription of their target genes. in this study, a total of 159 tfs were identified from a spontaneous late-ripening mutant 'fengwan' (c. sinensis l. osbeck) sweet orange (mt) and its wild-type counterpart ('fengjie 72-1', wt) along the ripening period via the transcription factor prediction of planttfdb 3.0. fifty-two dif ... | 2016 | 27104786 |
| integrated systems biology analysis of transcriptomes reveals candidate genes for acidity control in developing fruits of sweet orange (citrus sinensis l. osbeck). | organic acids, such as citrate and malate, are important contributors for the sensory traits of fleshy fruits. although their biosynthesis has been illustrated, regulatory mechanisms of acid accumulation remain to be dissected. to provide transcriptional architecture and identify candidate genes for citrate accumulation in fruits, we have selected for transcriptome analysis four varieties of sweet orange (citrus sinensis l. osbeck) with varying fruit acidity, succari (acidless), bingtang (low ac ... | 2016 | 27092171 |
| chemistry and pharmacology of citrus sinensis. | presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. natural products have been the source of new drugs since ancient times. plants are a good source of secondary metabolites which have been found to have beneficial properties. the present study is a review of the chemistry and pharmacology of citrus sinensis. this review reveals the therapeutic potential of c. sinensis as a source of natural compounds with important activities that are ... | 2016 | 27072414 |
| inhibiting ethylene perception with 1-methylcyclopropene triggers molecular responses aimed to cope with cell toxicity and increased respiration in citrus fruits. | the ethylene perception inhibitor 1-methylcyclopropene (1-mcp) has been critical in understanding the hormone's mode of action. however, 1-mcp may trigger other processes that could vary the interpretation of results related until now to ethylene, which we aim to understand by using transcriptomic analysis. transcriptomic changes in ethylene and 1-mcp-treated 'navelate' (citrus sinensis l. osbeck) oranges were studied in parallel with changes in ethylene production, respiration and peel damage. ... | 2016 | 26990405 |
| fragile sites of 'valencia' sweet orange (citrus sinensis) chromosomes are related with active 45s rdna. | citrus sinensis chromosomes present a morphological differentiation of bands after staining by the fluorochromes cma and dapi, but there is still little information on its chromosomal characteristics. in this study, the chromosomes in 'valencia' c. sinensis were analyzed by fluorescence in situ hybridization (fish) using telomere dna and the 45s rdna gene as probes combining cma/dapi staining, which showed that there were two fragile sites in sweet orange chromosomes co-localizing at distended 4 ... | 2016 | 26977938 |
| anatomical and physiological responses of citrus trees to varying boron availability are dependent on rootstock. | in citrus, water, nutrient transport and thereby fruit production, are influenced among other factors, by the interaction between rootstock and boron (b) nutrition. this study aimed to investigate how b affects the anatomical structure of roots and leaves as well as leaf gas exchange in sweet orange trees grafted on two contrasting rootstocks in response to b supply. plants grafted on swingle citrumelo or sunki mandarin were grown in a nutrient solution of varying b concentration (deficient, ade ... | 2016 | 26973670 |
| microrna regulatory mechanisms on citrus sinensis leaves to magnesium-deficiency. | magnesium (mg)-deficiency, which affects crop productivity and quality, widespreadly exists in many agricultural crops, including citrus. however, very limited data are available on mg-deficiency-responsive micrornas (mirnas) in higher plants. using illumina sequencing, we isolated 75 (73 known and 2 novel) up- and 71 (64 known and 7 novel) down-regulated mirnas from mg-deficient citrus sinensis leaves. in addition to the remarkable metabolic flexibility as indicated by the great alteration of m ... | 2016 | 26973661 |
| illumina microrna profiles reveal the involvement of mir397a in citrus adaptation to long-term boron toxicity via modulating secondary cell-wall biosynthesis. | the mechanisms underlying tolerance to b-toxicity in plants are still controversial. our previous studies indicated that b-toxicity is mainly limited to leaves in citrus and that alternations of cell-wall structure in vascular bundles are involved in tolerance to b-toxicity. here, mirnas and their expression patterns were first identified in b-treated citrus sinensis (tolerant) and c. grandis (intolerant) leaves via high-throughput sequencing. candidate mirnas were then verified with molecular a ... | 2016 | 26962011 |
| chemistry and pharmacology of citrus sinensis. | presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. natural products have been the source of new drugs since ancient times. plants are a good source of secondary metabolites which have been found to have beneficial properties. the present study is a review of the chemistry and pharmacology of citrus sinensis. this review reveals the therapeutic potential of c. sinensis as a source of natural compounds with important activities that are ... | 2016 | 26907240 |
| transmission rates of ‘ca. liberibacter asiaticus’ by asian citrus psyllid are enhanced by the presence and developmental stage of citrus flush. | asian citrus psyllid (diaphorina citri kuwayama) transmits a bacterium ‘candidatus liberibacter asiaticus’ (clas) putatively responsible for a devastating citrus disease known as asiatic huanglongbing (hlb) (citrus greening disease). the psyllid and disease have invaded many citrus-growing regions including the united states, where the disease is seriously jeopardizing the florida citrus industry. we recently concluded research that showed clas transmission rates are increased when citrus flush ... | 2016 | 26884596 |
| the use of laser light to enhance the uptake of foliar-applied substances into citrus (citrus sinensis) leaves. | uptake of foliar-applied substances across the leaf cuticle is central to world food production as well as for physiological investigations into phloem structure and function. yet, despite the presence of stomata, foliar application as a delivery system can be extremely inefficient due to the low permeability of leaf surfaces to polar compounds. | 2016 | 26819863 |
| the anticonvulsant activity of a flavonoid-rich extract from orange juice involves both nmda and gaba-benzodiazepine receptor complexes. | the usage of dietary supplements and other natural products to treat neurological diseases has been growing over time, and accumulating evidence suggests that flavonoids possess anticonvulsant properties. the aim of this study was to examine the effects of a flavonoid-rich extract from orange juice (oje) in some rodent models of epilepsy and to explore its possible mechanism of action. the genetically audiogenic seizures (ags)-susceptible dba/2 mouse, the pentylenetetrazole (ptz)-induced seizure ... | 2016 | 27657037 |
| yield, esterification degree and molecular weight evaluation of pectins isolated from orange and grapefruit peels under different conditions. | orange (citrus sinensis) and grapefruit (citrus paradise) peels were used as a source of pectin, which was extracted under different conditions. the peels are used under two states: fresh and residual (after essential oil extraction). organic acid (citric acid) and mineral acid (sulfuric acid) were used in the pectin extraction. the aim of this study is the evaluation the effect of extraction conditions on pectin yield, degree of esterification "de" and on molecular weight "mw". results showed t ... | 2016 | 27644093 |
| cspao4 of citrus sinensis functions in polyamine terminal catabolism and inhibits plant growth under salt stress. | polyamine oxidase (pao) is a key enzyme catalyzing polyamine catabolism leading to h2o2 production. we previously demonstrated that citrus sinensis contains six putative pao genes, but their functions are not well understood. in this work, we reported functional elucidation of cspao4 in polyamine catabolism and salt stress response. cspao4 was localized to the apoplast and used both spermidine (spd) and spermine (spm) as substrates for terminal catabolism. transgenic plants overexpressing cspao4 ... | 2016 | 27535697 |
| citrate accumulation-related gene expression and/or enzyme activity analysis combined with metabolomics provide a novel insight for an orange mutant. | 'hong anliu' (hal, citrus sinensis cv. hong anliu) is a bud mutant of 'anliu' (al), characterized by a comprehensive metabolite alteration, such as lower accumulation of citrate, high accumulation of lycopene and soluble sugars in fruit juice sacs. due to carboxylic acid metabolism connects other metabolite biosynthesis and/or catabolism networks, we therefore focused analyzing citrate accumulation-related gene expression profiles and/or enzyme activities, along with metabolic fingerprinting bet ... | 2016 | 27385485 |
| assessment of genetic diversity of tunisian orange, citrus sinensis (l.) osbeck using microsatellite (ssr) markers. | citrus are one of the most cultivated crops in the world. economically, they are very important fruit trees in tunisia. little is known about the genetic diversity of the tunisian citrus germplasm. exploring this diversity is a prerequisite for the identification and characterization of the local germplasm to circumvent and controlling genetic erosion caused by biotic and abiotic stress to aid its conservation and use. in the present study, we explored the genetic diversity of 20 tunisian orange ... | 2016 | 27323057 |
| a simple and rapid hplc-pda ms method for the profiling of citrus peels and traditional italian liquors. | a chromatographic method for the qualitative and quantitative characterization of peels and preparations based on different species of citrus was developed in order to obtain a complete profile of the constituents, including flavonoids and protoalkaloids. commercial peels of sweet orange, lemon, mandarin, and grapefruit were analyzed. seventeen constituents including flavanones, flavones, polymethoxyflavones, and protoalkaloids were identified by hplc-pda, hplc-ms, and hplc-ms/ms using a compari ... | 2016 | 27280933 |
| biolistic transformation of carrizo citrange (citrus sinensis osb. × poncirus trifoliata l. raf.). | the development of transgenic citrus plants by the biolistic method. a protocol for the biolistic transformation of epicotyl explants and transgenic shoot regeneration of immature citrange rootstock, cv. carrizo (citrus sinensis osb. × poncirus trifoliata l. raf.) and plant regeneration is described. immature epicotyl explants were bombarded with a vector containing the nptii selectable marker and the gfp reporter. the number of independent, stably transformed tissues/total number of explants, r ... | 2016 | 27277128 |
| homologues of cslob1 in citrus function as disease susceptibility genes in citrus canker. | the lateral organ boundary domain (lbd) genes encode a group of plant-specific proteins that function as transcription factors in the regulation of plant growth and development. citrus sinensis lateral organ boundary 1 (cslob1) is a member of the lbd family and functions as a disease susceptibility gene in citrus bacterial canker (cbc). thirty-four lbd members have been identified from the citrus sinensis genome. we assessed the potential for additional members of lbd genes in citrus to function ... | 2016 | 27276658 |
| antioxidant activity of oils extracted from orange (citrus sinensis) seeds. | due to the increasing production of food in the world with consequent increase of the production of waste, the importance of developing researches for its use is noticed. thus, the interest in vegetable oils with bioactive compounds, such as the ones extracted from fruit seeds, is growing. therefore, the present study aims to characterize the oils extracted from seeds of hamlin, natal, pera-rio and valencia orange varieties (citrus sinensis), as to the levels of total carotenoids, total phenolic ... | 2016 | 27254458 |
| tetraploidy enhances boron-excess tolerance in carrizo citrange (citrus sinensis l. osb. × poncirus trifoliata l. raf.). | tetraploidy modifies root anatomy which may lead to differentiated capacity to uptake and transport mineral elements. this work provides insights into physiological and molecular characters involved in boron (b) toxicity responses in diploid (2x) and tetraploid (4x) plants of carrizo citrange (citrus sinensis l. osb. × poncirus trifoliata l. raf.), a widely used citrus rootstock. with b excess, 2x plants accumulated more b in leaves than 4x plants, which accounted for their higher b uptake and r ... | 2016 | 27252717 |
| population fluctuation of ceratitis capitata (diptera: tephritidae) as a function of altitude in eastern guatemala. | population fluctuations of ceratitis capitata (wiedemann) were evaluated over a period of 12 mo in four altitudinal strata (400-750, 750-1,100, 1,100-1,450, and 1,450-1,800 meters above sea level, masl) in eastern guatemala. within each altitudinal range, sampling plots were established in coffee plantations and adjacent areas, in which jackson traps were set and baited with trimedlure. coffee berries and other host fruits were collected. population density was lowest at the 400-750 masl stratum ... | 2016 | 27247307 |
| phytohormone profiling of the sweet orange (citrus sinensis (l.) osbeck) leaves and roots using gc-ms-based method. | phytohormones mainly affect plant development and trigger varied responses to biotic and abiotic stresses. the sensitivity of methods used to profile phytohormones is a vital factor that affects the results. we used an improved gc-ms-based method in the selective ion-monitoring (sim) mode to study the phytohormone profiling in citrus tissues. one extraction solvent mixture and two derivatization reagents were used, methyl chloroformate (mcf) and n-methyl-n-(trimethylsilyl) trifluoroacetamide (ms ... | 2016 | 27224902 |
| effect of feeding dried sweet orange (citrus sinensis) peel and lemon grass (cymbopogon citratus) leaves on growth performance, carcass traits, serum metabolites and antioxidant status in broiler during the finisher phase. | the current experiment was conducted to evaluate the effects of feeding dried sweet orange peel (sop) and lemon grass leaves (lgl) as feed additives on broiler growth performance, serum metabolites, and antioxidant status. a total of 192-day-old (ross 308) broiler chickens were distributed randomly into 4 dietary treatments with 4 replicates per each treatment. the dietary treatments included a control diet without any feed additive (t1), a diet containing 0.8 % sop (t2), a diet containing 0.8 % ... | 2016 | 27209636 |
| citap2.10 activation of the terpene synthase cstps1 is associated with the synthesis of (+)-valencene in 'newhall' orange. | aroma is a vital characteristic that determines the quality and commercial value of citrus fruits, and characteristic volatiles have been analyzed in different citrus species. in sweet orange, citrus sinensis, the sesquiterpene (+)-valencene is a key volatile compound in the fruit peel. valencene synthesis is catalyzed by the terpene synthase cstps1, but the transcriptional mechanisms controlling its gene expression are unknown. here, the ap2/erf (apetala2/ethylene response factor) transcription ... | 2016 | 27194737 |