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construction of citrus gene coexpression networks from microarray data using random matrix theory.after the sequencing of citrus genomes, gene function annotation is becoming a new challenge. gene coexpression analysis can be employed for function annotation using publicly available microarray data sets. in this study, 230 sweet orange (citrus sinensis) microarrays were used to construct seven coexpression networks, including one condition-independent and six condition-dependent (citrus canker, huanglongbing, leaves, flavedo, albedo, and flesh) networks. in total, these networks contain 37 6 ...201526504573
high temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant pichia kudriavzevii kvmp10.this work explores the potential for the development of orange peel based ethanol bioprocesses through isolation of the thermotolerant pichia kudriavzevii kvmp10. a model solution of hydrolysed valencia orange peel was employed to determine the ethanologenic potential of the yeast, which was maximized at 42°c producing 54 g l(-1) of ethanol. the effect of orange peel oil on bioethanol formation was investigated at 30 and 42°c confirming that the minimum inhibitory peel oil content was 0·01% (v/v ...201626510181
boron-deficiency-responsive micrornas and their targets in citrus sinensis leaves.micrornas play important roles in the adaptive responses of plants to nutrient deficiencies. most research, however, has focused on nitrogen (n), phosphorus (p), sulfur (s), copper (cu) and iron (fe) deficiencies, limited data are available on the differential expression of mirnas and their target genes in response to deficiencies of other nutrient elements. in this study, we identified the known and novel mirnas as well as the boron (b)-deficiency-responsive mirnas from citrus leaves in order t ...201526538180
reduced susceptibility to xanthomonas citri in transgenic citrus expressing the fls2 receptor from nicotiana benthamiana.overexpression of plant pattern-recognition receptors by genetic engineering provides a novel approach to enhance plant immunity and broad-spectrum disease resistance. citrus canker disease associated with xanthomonas citri is one of the most important diseases damaging citrus production worldwide. in this study, we cloned the fls2 gene from nicotiana benthamiana cdna and inserted it into the binary vector pbinplus/ars to transform hamlin sweet orange and carrizo citrange. transgene presence was ...201626554734
an apple plus a brazil nut a day keeps the doctors away: antioxidant capacity of foods and their health benefits.antioxidant-rich foods scavenge free radicals and other reactive species, decreasing the risk of different non-communicable chronic diseases. the objective of this study was to review the content of total antioxidant capacity of commonly foods comparing with experimental data and to explore the health benefits due to foods with moderate to high tac. the tac was analytically measured using the "total antioxidant capacity" (nx2332) test from randox® (uk) by spectrometry at 600 nm. brazil nut (bert ...201626572874
root itraq protein profile analysis of two citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity.limited information is available on aluminum (al)-toxicity-responsive proteins in woody plant roots. seedlings of 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) were treated for 18 weeks with nutrient solution containing 0 (control) or 1.2 mm alcl3 · 6h2o (+al). thereafter, we investigated citrus root protein profiles using isobaric tags for relative and absolute quantification (itraq). the aims of this work were to determine the molecular mechanisms of plants to deal with al-tox ...201526573913
c- and o-glycosyl flavonoids in sanguinello and tarocco blood orange (citrus sinensis (l.) osbeck) juice: identification and influence on antioxidant properties and acetylcholinesterase activity.sanguinello and tarocco are the blood orange (citrus sinensis (l.) osbeck) cultivars most diffused worldwide. reversed phase liquid chromatography coupled with ms-ms analysis showed that these two varieties have a similar chromatographic pattern, characterised by the presence of c- and o-glycosyl flavonoids. of the two, sanguinello was found to be far richer in flavonoids than tarocco. in the juices, twelve individual components were identified for the first time, namely, four c-glycosyl flavone ...201626593535
identification, characterization and expression analysis of lineage-specific genes within sweet orange (citrus sinensis).with the availability of rapidly increasing number of genome and transcriptome sequences, lineage-specific genes (lsgs) can be identified and characterized. like other conserved functional genes, lsgs play important roles in biological evolution and functions.201526597278
hesperetin attenuates ventilator-induced acute lung injury through inhibition of nf-κb-mediated inflammation.hesperetin, a major bioflavonoid in sweet oranges and lemons, has been reported to have anti-inflammatory properties. however, the effect of hesperetin on ventilator-induced acute lung injury has not been studied. in present study, we investigated the protective effect of hesperetin on ventilator-induced acute lung injury in rats. rats were orally administered hesperetin (10, 20, or 40mg/kg) two hour before acute lung injury was induced by mechanical ventilation. rats were then randomly divided ...201526610718
thiamethoxam and imidacloprid drench applications on sweet orange nursery trees disrupt the feeding and settling behaviour of diaphorina citri (hemiptera: liviidae).chemical control is the method most used for management of diaphorina citri, the vector of the phloem-limited bacteria associated with citrus huanglongbing (hlb) disease. the objectives of this study were to determine the influence of soil-drench applications of neonicotinoids (thiamethoxam and imidacloprid) on the probing behaviour of d. citri on citrus nursery trees, using the electrical penetration graph (epg) technique, and to measure the d. citri settling behaviour after probing on citrus n ...201626694803
coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings.volatile organic compounds (vocs) are off-gassed from all living organisms and represent end products of metabolic pathways within the system. in agricultural systems, these vocs can provide important information on plant health and can ordinarily be measured non-invasively without harvesting tissue from the plants. previously we reported a portable gas chromatography/differential mobility spectrometry (gc/dms) system that could distinguish voc profiles of pathogen-infected citrus from healthy t ...201626695246
late embryogenesis abundant (lea) constitutes a large and diverse family of proteins involved in development and abiotic stress responses in sweet orange (citrus sinensis l. osb.).late embryogenesis abundant (lea) proteins are an ubiquitous group of polypeptides that were first described to accumulate during plant seed dehydration, at the later stages of embryogenesis. since then they have also been recorded in vegetative plant tissues experiencing water limitation and in anhydrobiotic bacteria and invertebrates and, thereby, correlated with the acquisition of desiccation tolerance. this study provides the first comprehensive study about the lea gene family in sweet orang ...201526700652
additive roles of pthas in bacterial growth and pathogenicity associated with nucleotide polymorphisms in effector-binding elements of citrus canker susceptibility genes.citrus canker, caused by xanthomonas citri, affects most commercial citrus varieties. all x. citri strains possess at least one transcription activator-like effector of the ptha family that activates host disease susceptibility (s) genes. the x. citri strain 306 encodes four ptha effectors; nevertheless, only ptha4 is known to elicit cankers on citrus. as none of the pthas act as avirulence factors on citrus, we hypothesized that pthas 1-3 might also contribute to pathogenicity on certain hosts. ...201626709719
[effect of characteristic variable extraction on accuracy of cu in navel orange peel by libs].heavy metals pollution in foodstuffs is more and more serious. it is impossible to satisfy the modern agricultural development by conventional chemical analysis. laser induced breakdown spectroscopy (libs) is an emerging technology with the characteristic of rapid and nondestructive detection. but libs' s repeatability, sensitivity and accuracy has much room to improve. in this work, heavy metal cu in gannan navel orange which is the jiangxi specialty fruit will be predicted by libs. firstly, th ...201526717771
comparative transcriptional profiling of orange fruit in response to the biocontrol yeast kloeckera apiculata and its active compounds.the yeast kloeckera apiculata strain 34-9 is an antagonist that shows biological control activity against the postharvest fungal pathogens of citrus. an antifungal compound, 2-phenylethanol (pea), has been identified from the extract of k. apiculata. to better understand the molecular processes underlying the response of citrus fruit tissue to k. apiculata, the extract and pea, microarray analyses were performed on navel oranges using an affymetrix citrus genechip.201626725242
spatio-temporal distribution and natural variation of metabolites in citrus fruits.to study the natural variation and spatio-temporal accumulation of citrus metabolites, liquid chromatography tandem mass spectrometry (lc-ms) based metabolome analysis was performed on four fruit tissues (flavedo, albedo, segment membrane and juice sacs) and different citrus species (lemon, pummelo and grapefruit, sweet orange and mandarin). using a non-targeted metabolomics approach, more than 2000 metabolite signals were detected, from which more than 54 metabolites, including amino acids, fla ...201626775938
temporal transcription profiling of sweet orange in response to ptha4-mediated xanthomonas citri subsp. citri infection.citrus canker, caused by xanthomonas citri subsp. citri, is a devastating disease of most commercial citrus varieties. in our previous study, we analyzed the transcriptional response of 'valencia' sweet orange to x. citri subsp. citri wild-type and ptha4 mutant infection at 48 h postinoculation (hpi). using microarray analysis, two ptha4 targets, cslob1 and cssweet1, were identified. we have shown that ptha4 binds to the effector binding element (ebe) of cslob1 and activates gene expression of t ...201626780431
selection of reference genes for expression analyses of red-fleshed sweet orange (citrus sinensis).red-fleshed oranges (citrus sinensis) contain high levels of carotenoids and lycopene. the growing consumer demand for products with health benefits has increased interest in these types of citrus cultivars as a potential source of nutraceuticals. however, little is known about the physiology of these cultivars under brazilian conditions. transcriptome and gene expression analyses are important tools in the breeding and management of red-fleshed sweet orange cultivars. reverse transcription quan ...201526782492
hplc-dad phenolic characterization and antioxidant activities of ripe and unripe sweet orange peels.phenolic compounds of unripe and ripe sweet orange peels were determined using a high-performance liquid chromatography separation method with diode array detector (hplc-dad). the in vitro antioxidant properties and the ec50 (concentration required to obtain a 50% antioxidant effect) values were also determined. the predominant phenolic compounds were quercitrin, rutin, and quercetin with values of 18.77 ± 0.01 mg/ml, 18.65 ± 0.03 mg/ml, and 10.39 ± 0.01 mg/ml respectively in unripe orange peel ...201526783839
regulation of cuticle formation during fruit development and ripening in 'newhall' navel orange (citrus sinensis osbeck) revealed by transcriptomic and metabolomic profiling.fruit cuticle, which is composed of cutin and wax and biosynthesized during fruit development, plays important roles in the prevention of water loss and the resistance to pathogen infection during fruit development and postharvest storage. however, the key factors and mechanisms regarding the cuticle biosynthesis in citrus fruits are still unclear. here, fruit cuticle of 'newhall' navel orange (citrus sinensis osbeck) was studied from the stage of fruit expansion to postharvest storage from the ...201626795158
assessment of navel oranges, clementine tangerines, and rutaceous fruits as hosts of bactrocera cucurbitae and bactrocera latifrons (diptera: tephritidae).export of citrus spp. fruits may require risk mitigation measures if grown in areas with established tephritid fruit fly (diptera: tephritidae) populations capable of infesting the fruits. the host status of citrus spp. fruits is unclear for two tephritid fruit fly species whose geographic ranges have expanded in recent years: melon fly, bactrocera cucurbitae (cocquillett), and bactrocera latifrons (hendel). in no choice cage infestation studies, b. latifrons oviposited into intact and punctured ...201526816484
the use of laser light to enhance the uptake of foliar-applied substances into citrus (citrus sinensis) leaves.uptake of foliar-applied substances across the leaf cuticle is central to world food production as well as for physiological investigations into phloem structure and function. yet, despite the presence of stomata, foliar application as a delivery system can be extremely inefficient due to the low permeability of leaf surfaces to polar compounds.201626819863
potent inhibition of pseudogymnoascus destructans, the causative agent of white-nose syndrome in bats, by cold-pressed, terpeneless, valencia orange oil.the causative agent of white-nose syndrome (wns), pseudogymnoascus destructans, has been shown to be fatal to several species of bats in north america. to date, no compounds or chemical control measures have been developed which eliminates the growth of the fungus in the environment or in affected animals. in the current study, we evaluated the activity of cold-pressed, terpeneless orange oil (cpt) against multiple isolates of p. destructans in vitro. for all assays, a modified kirby-bauer disk ...201626849057
antimicrobial effects of citrus sinensis peel extracts against dental caries bacteria: an in vitro study.ethnomedicine is gaining admiration since years but still there is abundant medicinal flora which is unrevealed through research. the study was conducted to assess the in vitro antimicrobial potential and also determine the minimum inhibitory concentration (mic) of citrus sinensis peel extracts with a view of searching a novel extract as a remedy for dental caries pathogens.201626855710
transmission rates of ‘ca. liberibacter asiaticus’ by asian citrus psyllid are enhanced by the presence and developmental stage of citrus flush.asian citrus psyllid (diaphorina citri kuwayama) transmits a bacterium ‘candidatus liberibacter asiaticus’ (clas) putatively responsible for a devastating citrus disease known as asiatic huanglongbing (hlb) (citrus greening disease). the psyllid and disease have invaded many citrus-growing regions including the united states, where the disease is seriously jeopardizing the florida citrus industry. we recently concluded research that showed clas transmission rates are increased when citrus flush ...201626884596
chemistry and pharmacology of citrus sinensis.presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. natural products have been the source of new drugs since ancient times. plants are a good source of secondary metabolites which have been found to have beneficial properties. the present study is a review of the chemistry and pharmacology of citrus sinensis. this review reveals the therapeutic potential of c. sinensis as a source of natural compounds with important activities that are ...201626907240
larvicidal potential of essential oils against musca domestica and anopheles stephensi.the larvicidal activity of mentha piperita, cymbopogan citratus (lemongrass), eucalyptus globulus and citrus sinensis (orange) essential oils and their combinations was evaluated against musca domestica (housefly) and anopheles stephensi (mosquitoes) through contact toxicity assay. among all the tested essential oils/combinations, me. piperita was found to be the most effective larvicidal agent against mu. domestica and an. stephensi with lc50 values of 0.66 μl/cm(2) and 44.66 ppm, respectively, ...201626920567
comparison of the composition and antimicrobial activities of the essential oils of green branches and leaves of egyptian navel orange (citrus sinensis (l.) osbeck var. malesy).the essential oils isolated from the leaves and green branches of the egyptian navel orange trees were analyzed by gc and gc/ms. a total of 33 and 24 compounds were identified from the oils of the leaves and branches accounting for 96.0% and 97.9%, respectively, of the total detected constituents. the major ones were sabinene (36.5; 33.0%), terpinen-4-ol (8.2; 6.2%), δ-3-carene (7.0; 9.4%), limonene (6.8; 18.7%), trans-ocimene (6.7; 6.1%), and β-myrcene (4.5; 4.4%). the antimicrobial activities ...201626948682
illumina microrna profiles reveal the involvement of mir397a in citrus adaptation to long-term boron toxicity via modulating secondary cell-wall biosynthesis.the mechanisms underlying tolerance to b-toxicity in plants are still controversial. our previous studies indicated that b-toxicity is mainly limited to leaves in citrus and that alternations of cell-wall structure in vascular bundles are involved in tolerance to b-toxicity. here, mirnas and their expression patterns were first identified in b-treated citrus sinensis (tolerant) and c. grandis (intolerant) leaves via high-throughput sequencing. candidate mirnas were then verified with molecular a ...201626962011
microrna regulatory mechanisms on citrus sinensis leaves to magnesium-deficiency.magnesium (mg)-deficiency, which affects crop productivity and quality, widespreadly exists in many agricultural crops, including citrus. however, very limited data are available on mg-deficiency-responsive micrornas (mirnas) in higher plants. using illumina sequencing, we isolated 75 (73 known and 2 novel) up- and 71 (64 known and 7 novel) down-regulated mirnas from mg-deficient citrus sinensis leaves. in addition to the remarkable metabolic flexibility as indicated by the great alteration of m ...201626973661
anatomical and physiological responses of citrus trees to varying boron availability are dependent on rootstock.in citrus, water, nutrient transport and thereby fruit production, are influenced among other factors, by the interaction between rootstock and boron (b) nutrition. this study aimed to investigate how b affects the anatomical structure of roots and leaves as well as leaf gas exchange in sweet orange trees grafted on two contrasting rootstocks in response to b supply. plants grafted on swingle citrumelo or sunki mandarin were grown in a nutrient solution of varying b concentration (deficient, ade ...201626973670
fragile sites of 'valencia' sweet orange (citrus sinensis) chromosomes are related with active 45s rdna.citrus sinensis chromosomes present a morphological differentiation of bands after staining by the fluorochromes cma and dapi, but there is still little information on its chromosomal characteristics. in this study, the chromosomes in 'valencia' c. sinensis were analyzed by fluorescence in situ hybridization (fish) using telomere dna and the 45s rdna gene as probes combining cma/dapi staining, which showed that there were two fragile sites in sweet orange chromosomes co-localizing at distended 4 ...201626977938
[prediction of cadmium content in the leaves of navel orange in heavy metal contaminated soil using vis-nir reflectance spectroscopy].visual and near-infrared (vis-nir) reflectance spectroscopy had been used widely in monitoring agricultural pollution in recent years, however, it was rarely applied in monitoring the contamination of heavy metal in orchards. in the present paper, newhall navel orange (citrus sinensis [l.] osbeck cv. newhall) were cultivated in the potted soil contaminated with cadmium (cd) at different levels, and the spectral reflectance and cd content in the leaves were measured simultaneously at different gr ...201526978924
raman spectroscopy an option for the early detection of citrus huanglongbing.this research describes the application of portable field raman spectroscopy combined with a statistical analysis of the resulting spectra, employing principal component analysis (pca) and linear discriminant analysis (lda), in which we determine that this method provides a high degree of reliability in the early detection of huanglongbing (hlb) on sweet orange, disease caused by the bacteria candidatus liberibacter asiaticus. symptomatic and asymptomatic plant samples of sweet orange (citrus si ...201626988658
inhibiting ethylene perception with 1-methylcyclopropene triggers molecular responses aimed to cope with cell toxicity and increased respiration in citrus fruits.the ethylene perception inhibitor 1-methylcyclopropene (1-mcp) has been critical in understanding the hormone's mode of action. however, 1-mcp may trigger other processes that could vary the interpretation of results related until now to ethylene, which we aim to understand by using transcriptomic analysis. transcriptomic changes in ethylene and 1-mcp-treated 'navelate' (citrus sinensis l. osbeck) oranges were studied in parallel with changes in ethylene production, respiration and peel damage. ...201626990405
involvement of an ethylene response factor in chlorophyll degradation during citrus fruit degreening.chlorophyll degradation naturally occurs during plant senescence. however, in fruit such as citrus, it is a positive characteristic, as degreening is an important colour development contributing to fruit quality. in the present work, citrus sinensis osbeck, cv. newhall fruit was used as a model for chlorophyll degradation. an ethylene response factor, citerf13, was isolated and its transcriptional changes were closely correlated with fruit peel degreening during development or in response to eth ...201627037684
distribution of 'candidatus liberibacter asiaticus' above and below ground in texas citrus.detection of 'candidatus liberibacter asiaticus' represents one of the most difficult, yet critical, steps of controlling huanglongbing disease. efficient detection relies on understanding the underlying distribution of bacteria within trees. to that end, we studied the distribution of 'ca. l. asiaticus' in leaves of 'rio red' grapefruit trees and in roots of 'valencia' sweet orange trees grafted onto sour orange rootstock. we performed two sets of leaf collection on grapefruit trees; the first ...201627050571
modification of the ptha4 effector binding elements in type i cslob1 promoter using cas9/sgrna to produce transgenic duncan grapefruit alleviating xccδptha4:dcslob1.3 infection.citrus canker caused by xanthomonas citri subspecies citri (xcc) is a severe disease for most commercial citrus cultivars and responsible for significant economic losses worldwide. generating canker-resistant citrus varieties will provide an efficient and sustainable solution to control citrus canker. here, we report our progress in generating canker-resistant grapefruit by modifying the ptha4 effector binding elements (ebes) in the cslob1 promoter (ebeptha4 -cslobp) of the cslob1 (citrus sinens ...201627071672
chemistry and pharmacology of citrus sinensis.presently the search for new drugs from natural resources is of growing interest to the pharmaceutical industry. natural products have been the source of new drugs since ancient times. plants are a good source of secondary metabolites which have been found to have beneficial properties. the present study is a review of the chemistry and pharmacology of citrus sinensis. this review reveals the therapeutic potential of c. sinensis as a source of natural compounds with important activities that are ...201627072414
integrated systems biology analysis of transcriptomes reveals candidate genes for acidity control in developing fruits of sweet orange (citrus sinensis l. osbeck).organic acids, such as citrate and malate, are important contributors for the sensory traits of fleshy fruits. although their biosynthesis has been illustrated, regulatory mechanisms of acid accumulation remain to be dissected. to provide transcriptional architecture and identify candidate genes for citrate accumulation in fruits, we have selected for transcriptome analysis four varieties of sweet orange (citrus sinensis l. osbeck) with varying fruit acidity, succari (acidless), bingtang (low ac ...201627092171
genome-wide identification of the transcription factors involved in citrus fruit ripening from the transcriptomes of a late-ripening sweet orange mutant and its wild type.fruit ripening is a genetically programmed process. transcription factors (tfs) play key roles in plant development and ripening by temporarily and spatially regulating the transcription of their target genes. in this study, a total of 159 tfs were identified from a spontaneous late-ripening mutant 'fengwan' (c. sinensis l. osbeck) sweet orange (mt) and its wild-type counterpart ('fengjie 72-1', wt) along the ripening period via the transcription factor prediction of planttfdb 3.0. fifty-two dif ...201627104786
antihyperlipidemic effects of citrus sinensis, citrus paradisi, and their combinations.hyperlipidemia, extensively contributes in the progression of coronary heart diseases and atherosclerosis, but may be managed through alterations in the nutritional pattern. several studies show that diet rich in polyphenols and antioxidants have antiatherogenic effects. citrus sinensis and citrus paradisi are widely known for health benefits and have found to produce antioxidant, anti-inflammatory, and hypolipidemic effects, hence current research was planned to determine the hypolipidemic effe ...201727134462
antimycotic activity and genotoxic evaluation of citrus sinensis and citrus latifolia essential oils.the aim of this study was to evaluate the antifungal activity of essential oils (eos) of citrus sinensis (c. sinensis) and citrus latifolia (c. latifolia) against five candida species: candida albicans, candida tropicalis, candida glabrata, candida lusitaniae and candida guilliermondii; and perform its genotoxic evaluation. the eos of c. sinensis and c. latifolia were obtained from the peel by hydro-distillation. the major components determined by gc-ms were in c. sinensis, d-limonene (96%) and ...201627137128
population dynamics of chaff scale, parlatoria pergandii comstock in comparative analysis of fluctuating population densities on three citrus varieties in el-beheira governorate, egypt.investigation throughout the subsequent years of 2011-2012 and 2012-2013 indicated that chaff scale, p. pegandii is an important economic pest on citrus trees in abou el-matamer area, el-beheira governorate, egypt. it has been recorded in relatively high rates all over the year on three citrus varieties called succari, baladi and navel oranges. field observation and recorded data revealed that the common abundance was recorded for chaff scale on succari oranges followed by navel oranges then the ...201527145572
anti-inflammatory effects of citrus sinensis l., citrus paradisi l. and their combinations.citrus bioflavonoids embrace a wide group of phenolic compounds effecting the production and scavenging of reactive oxygen species and the processes relating free radical-mediated injury. keeping in view of the antioxidant and anti-inflammatory properties of citrus sinensis and citrus paradisi, present study was undertaken to explore the effects of c. sinensis (orange juice) and c. paradisi (grapefruit juice) at three different doses alone and their two combinations with the objective to examine ...201627166529
transcriptome analysis of sweet orange trees infected with 'candidatus liberibacter asiaticus' and two strains of citrus tristeza virus.huanglongbing (hlb) and tristeza, are diseases of citrus caused by a member of the α-proteobacteria, 'candidatus liberibacter asiaticus' (calas), and citrus tristeza virus (ctv) respectively. hlb is a devastating disease, but ctv strains vary from very severe to very mild. both calas and ctv are phloem-restricted. the calas-b232 strain and ctv-b6 cause a wide range of severe and similar symptoms. the mild strain ctv-b2 doesn't induce significant symptoms or damage to plants.201627169471
nucleotides, micro- and macro-nutrients, limonoids, flavonoids, and hydroxycinnamates composition in the phloem sap of sweet orange.currently, the global citrus production is declining due to the spread of huanglongbing (hlb). hlb, otherwise known as citrus greening, is caused by candidatus liberibacter asiaticus (clas) and is transmitted by the asian citrus psyllids (acp), diaphorina citri kuwayama. acp transmits clas bacterium while feeding on the citrus phloem sap. multiplication of clas in the phloem of citrus indicates that the sap contains all the essential nutrients needed for clas. in this study, we investigated the ...201627171979
the wrky transcription factor family in citrus: valuable and useful candidate genes for citrus breeding.wrky transcription factors belong to a large family of plant transcriptional regulators whose members have been reported to be involved in a wide range of biological roles including plant development, adaptation to environmental constraints and response to several diseases. however, little or poor information is available about wrky's in citrus. the recent release of completely assembled genomes sequences of citrus sinensis and citrus clementina and the availability of ests sequences from other ...201627193354
resistance of citrus and related genera to diaphorina citri kuwayama (hemiptera: liviidae).the present study was developed to evaluate the resistance of the following genotypes of citrus and related genera to this pest: 'pera,' 'natal', and 'washington navel' oranges (citrus sinensis), 'marsh seedless' grapefruit (citrus paradisi), hardy orange 'rubidoux' (poncirus trifoliata), kumquat (fortunella margarita swingle), citrumelo 'swingle' (c. paradisi x p. trifoliata), and citrange 'troyer' (p. trifoliata x c. sinensis). the experiments were performed in greenhouses with plants grafted ...201427193957
citap2.10 activation of the terpene synthase cstps1 is associated with the synthesis of (+)-valencene in 'newhall' orange.aroma is a vital characteristic that determines the quality and commercial value of citrus fruits, and characteristic volatiles have been analyzed in different citrus species. in sweet orange, citrus sinensis, the sesquiterpene (+)-valencene is a key volatile compound in the fruit peel. valencene synthesis is catalyzed by the terpene synthase cstps1, but the transcriptional mechanisms controlling its gene expression are unknown. here, the ap2/erf (apetala2/ethylene response factor) transcription ...201627194737
effect of feeding dried sweet orange (citrus sinensis) peel and lemon grass (cymbopogon citratus) leaves on growth performance, carcass traits, serum metabolites and antioxidant status in broiler during the finisher phase.the current experiment was conducted to evaluate the effects of feeding dried sweet orange peel (sop) and lemon grass leaves (lgl) as feed additives on broiler growth performance, serum metabolites, and antioxidant status. a total of 192-day-old (ross 308) broiler chickens were distributed randomly into 4 dietary treatments with 4 replicates per each treatment. the dietary treatments included a control diet without any feed additive (t1), a diet containing 0.8 % sop (t2), a diet containing 0.8 % ...201627209636
phytohormone profiling of the sweet orange (citrus sinensis (l.) osbeck) leaves and roots using gc-ms-based method.phytohormones mainly affect plant development and trigger varied responses to biotic and abiotic stresses. the sensitivity of methods used to profile phytohormones is a vital factor that affects the results. we used an improved gc-ms-based method in the selective ion-monitoring (sim) mode to study the phytohormone profiling in citrus tissues. one extraction solvent mixture and two derivatization reagents were used, methyl chloroformate (mcf) and n-methyl-n-(trimethylsilyl) trifluoroacetamide (ms ...201627224902
population fluctuation of ceratitis capitata (diptera: tephritidae) as a function of altitude in eastern guatemala.population fluctuations of ceratitis capitata (wiedemann) were evaluated over a period of 12 mo in four altitudinal strata (400-750, 750-1,100, 1,100-1,450, and 1,450-1,800 meters above sea level, masl) in eastern guatemala. within each altitudinal range, sampling plots were established in coffee plantations and adjacent areas, in which jackson traps were set and baited with trimedlure. coffee berries and other host fruits were collected. population density was lowest at the 400-750 masl stratum ...201627247307
tetraploidy enhances boron-excess tolerance in carrizo citrange (citrus sinensis l. osb. × poncirus trifoliata l. raf.).tetraploidy modifies root anatomy which may lead to differentiated capacity to uptake and transport mineral elements. this work provides insights into physiological and molecular characters involved in boron (b) toxicity responses in diploid (2x) and tetraploid (4x) plants of carrizo citrange (citrus sinensis l. osb. × poncirus trifoliata l. raf.), a widely used citrus rootstock. with b excess, 2x plants accumulated more b in leaves than 4x plants, which accounted for their higher b uptake and r ...201627252717
antioxidant activity of oils extracted from orange (citrus sinensis) seeds.due to the increasing production of food in the world with consequent increase of the production of waste, the importance of developing researches for its use is noticed. thus, the interest in vegetable oils with bioactive compounds, such as the ones extracted from fruit seeds, is growing. therefore, the present study aims to characterize the oils extracted from seeds of hamlin, natal, pera-rio and valencia orange varieties (citrus sinensis), as to the levels of total carotenoids, total phenolic ...201627254458
major components of orange oil inhibit staphylococcus aureus growth and biofilm formation, and alter its virulence factors.bovine mastitis is a costly disease in the dairy industry and does not always respond to antibiotic treatment. the major components of terpeneless, cold-pressed valencia orange oil - citral, linalool, decanal and valencene - were examined as potential alternative treatments for staphylococcus aureus-associated mastitis. the minimum inhibitory concentration (mic) of all four components against s. aureus was determined after incubation for 24 h. growth inhibition assays were performed for all effe ...201627259704
homologues of cslob1 in citrus function as disease susceptibility genes in citrus canker.the lateral organ boundary domain (lbd) genes encode a group of plant-specific proteins that function as transcription factors in the regulation of plant growth and development. citrus sinensis lateral organ boundary 1 (cslob1) is a member of the lbd family and functions as a disease susceptibility gene in citrus bacterial canker (cbc). thirty-four lbd members have been identified from the citrus sinensis genome. we assessed the potential for additional members of lbd genes in citrus to function ...201627276658
biolistic transformation of carrizo citrange (citrus sinensis osb. × poncirus trifoliata l. raf.).the development of transgenic citrus plants by the biolistic method. a protocol for the biolistic transformation of epicotyl explants and transgenic shoot regeneration of immature citrange rootstock, cv. carrizo (citrus sinensis osb. × poncirus trifoliata l. raf.) and plant regeneration is described. immature epicotyl explants were bombarded with a vector containing the nptii selectable marker and the gfp reporter. the number of independent, stably transformed tissues/total number of explants, r ...201627277128
sterol composition in the pollens of citrus unshiu marcov. and citrus sinensis osbeck. 199227280669
a simple and rapid hplc-pda ms method for the profiling of citrus peels and traditional italian liquors.a chromatographic method for the qualitative and quantitative characterization of peels and preparations based on different species of citrus was developed in order to obtain a complete profile of the constituents, including flavonoids and protoalkaloids. commercial peels of sweet orange, lemon, mandarin, and grapefruit were analyzed. seventeen constituents including flavanones, flavones, polymethoxyflavones, and protoalkaloids were identified by hplc-pda, hplc-ms, and hplc-ms/ms using a compari ...201627280933
identification of potential targets for an anticoagulant pectin.heparin is a sulfated polysaccharide of animal origin showing excellent anticoagulant properties. although it strongly inhibits the coagulation cascade, its interaction with multiple sites results in several side effects. an ideal alternative compound should not only possess anticoagulant and antithrombotic activities, but also provide specific binding to components of the coagulation cascade to decrease side effects and facilitate the control of pharmacologic actions in patient's body. in this ...201727318178
fluorescent carbon nanoparticles from citrus sinensis as efficient sorbents for pollutant dyes.here, we report a simple, green and economic process for the synthesis of highly fluorescent carbon nanoparticles (cps) through low-temperature carbonization of a fruit waste, citrus sinensis peel. this approach allows the large-scale production of aqueous cps dispersions without any additives and post-treatment processes. the as-prepared cps were of small particle size, exhibited bright blue fluorescence under uv irradiation (λmax  = 365 nm) with excellent colloidal stability in water. the chem ...201727321808
the in vitro effect of selected essential oils on the growth and mycotoxin production of aspergillus species.the aim of the present study was to assess the antifungal and anti-toxinogenic activity of 15 essential oils (eos) against three fungi of the genus aspergillus (a. parasiticus kmi-227-lr, a. parasiticus kmi-220-lr and a. flavus kmi-202-lr). the minimum inhibitory doses (mids) of the tested essential oils and their antifungal activity were determined using the micro-atmosphere method. the original commercial essential oil samples of jasminum officinale l., thymus vulgaris l., syzygium aromaticum ...201627322876
assessment of genetic diversity of tunisian orange, citrus sinensis (l.) osbeck using microsatellite (ssr) markers.citrus are one of the most cultivated crops in the world. economically, they are very important fruit trees in tunisia. little is known about the genetic diversity of the tunisian citrus germplasm. exploring this diversity is a prerequisite for the identification and characterization of the local germplasm to circumvent and controlling genetic erosion caused by biotic and abiotic stress to aid its conservation and use. in the present study, we explored the genetic diversity of 20 tunisian orange ...201627323057
comparing the use of laboratory-reared and field-collected thaumatotibia leucotreta (lepidoptera: tortricidae) larvae for demonstrating efficacy of postharvest cold treatments in citrus fruit.some of south africa's export markets require postharvest cold treatment of citrus fruit for phytosanitary risk mitigation for thaumatotibia leucotreta (meyrick) (lepidoptera: tortricidae). an alternative to a standalone cold treatment may be a reduced intensity cold treatment as a step in a systems approach. for cold treatment trials, large numbers of larvae are required. due to recent dramatic improvement of t. leucotreta control in the field, sufficient naturally infested citrus fruit are no ...201627341890
hesperidin alleviates rat postoperative ileus through anti-inflammation and stimulation of ca(2+)-dependent myosin phosphorylation.postoperative ileus (poi) is a postoperative dysmotility disorder of gastrointestinal tract, which remains one of the most perplexing problems in medicine. in the present study we investigated the effects of hesperidin, a major flavonoid in sweet oranges and lemons, on poi in rats.201627345626
an engineered promoter driving expression of a microbial avirulence gene confers recognition of tal effectors and reduces growth of diverse xanthomonas strains in citrus.xanthomonas citri ssp. citri (x. citri), causal agent of citrus canker, uses transcription activator-like effectors (tales) as major pathogenicity factors. tales, which are delivered into plant cells through the type iii secretion system (t3ss), interact with effector binding elements (ebes) in host genomes to activate the expression of downstream susceptibility genes to promote disease. predictably, tales bind ebes in host promoters via known combinations of tale amino acids to dna bases, known ...201627362693
chemical composition and anti-inflammatory effects of essential oil from hallabong flower.a number of essential oils derived from plants are claimed to have several medicinal functions, including anti-cancer and anti-inflammation effects. however, the chemical composition and biological activities of flower-derived components have not been sufficiently characterized. therefore, we investigated the composition of essential oils from hallabong flower [(citrus unshiu marcov × citrus sinensis osbeck) × citrus reticulata blanco] and their anti-inflammatory effects. hydro-distilled essenti ...201327366141
larvicidal activity of syzygium aromaticum (l.) merr and citrus sinensis (l.) osbeck essential oils and their antagonistic effects with temephos in resistant populations of aedes aegypti.environmentally friendly botanical larvicides are commonly considered as an alternative to synthetic larvicides against aedes aegypti linn. in addition, mosquito resistance to currently used larvicides has motivated research to find new compounds acting via different mechanisms of action, with the goal of controlling the spread of mosquitos. essential oils have been widely studied for this purpose. this work aims to evaluate the larvicidal potential of syzygium aromaticum and citrus sinensis ess ...027384083
citrate accumulation-related gene expression and/or enzyme activity analysis combined with metabolomics provide a novel insight for an orange mutant.'hong anliu' (hal, citrus sinensis cv. hong anliu) is a bud mutant of 'anliu' (al), characterized by a comprehensive metabolite alteration, such as lower accumulation of citrate, high accumulation of lycopene and soluble sugars in fruit juice sacs. due to carboxylic acid metabolism connects other metabolite biosynthesis and/or catabolism networks, we therefore focused analyzing citrate accumulation-related gene expression profiles and/or enzyme activities, along with metabolic fingerprinting bet ...201627385485
effect of essential oils on meat and fat qualities of crossbred young bulls finished in feedlots.twenty-seven animals (½ angus - ½ nellore) were fed for four months with one of the following diets: without addition of essential oils (e0.0), with 3.5 (e3.5) or 7 (e7.0) g/animal/day of an essential oil blend (oregano, garlic, lemon, rosemary, thyme, eucalyptus and sweet orange). chemical composition, fatty acid profile and meat color were evaluated in longissimus muscle. in addition, the effects of aging (one, seven and 14days) on the meat water holding capacity, texture and lipid oxidation w ...201627388818
[study on the rapid detection of triazophos residues in flesh of navel orange by using surface-enhanced raman scattering].surface enhanced raman spectroscopy (sers) and quick pre-treatment technology were used to detect triazophos residues in flesh of navel orange. quantitative analysis model was developed by partial least squares (pls) algorithm. sers of different concentration (0.5 to 20 mg x l(-1)) triazophos juice solution with flesh extract as the matrix were collected by laser raman spectrometer. three preprocessing methods such as normalization, msc and snv were used to optimize raman signals and pls models ...201627400516
laser-induced fluorescence spectroscopy (lifs) for discrimination of genetically close sweet orange accessions ( citrus sinensis l. osbeck).although there is substantial diversity among cultivated sweet oranges genotypes with respect to morphological, physiological, and agronomic traits, very little variation at dna level has been observed. it is possible that this low dna molecular variability is due to a narrow genetic basis commonly observed in this citrus group. the most different morphological characters observed were originated through mutations, which are maintained by vegetative propagation. despite all molecular tools avail ...201727402688
costs and benefits of insecticide and foliar nutrient applications to huanglongbing-infected citrus trees.the asian citrus psyllid (acp), diaphorina citri kuwayama (hemiptera: psyllidae), vectors candidatus liberibacter asiaticus, which causes huanglongbing (hlb). in florida, hlb incidence is approaching 100% statewide. yields have decreased and production costs have increased since 2005. despite this, some growers are maintaining a level of production and attribute this in part to aggressive psyllid control and foliar nutrition sprays. however, the value of these practices is debated. a replicated ...201727427170
repellent activity of botanical oils against asian citrus psyllid, diaphorina citri (hemiptera: liviidae).the asian citrus psyllid, diaphorina citri kuwayama, is the insect vector of the pathogen causing huanglongbing. we selected three botanical oils to evaluate behavioral activity against d. citri. in laboratory olfactometer assays, fir oil was repellent to d. citri females, while litsea and citronella oils elicited no response from d. citri females. in choice settling experiments, d. citri settled almost completely on control plants rather than on plants treated with fir oil at a 9.5 mg/day relea ...201627429006
inactivation of plant-pathogenic fungus colletotrichum acutatum with natural plant-produced photosensitizers under solar radiation.the increasing tolerance to currently used fungicides and the need for environmentally friendly antimicrobial approaches have stimulated the development of novel strategies to control plant-pathogenic fungi such as antimicrobial phototreatment (apt). we investigated the in vitro apt of the plant-pathogenic fungus colletotrichum acutatum with furocoumarins and coumarins and solar radiation. the compounds used were: furocoumarins 8-methoxypsoralen (8-mop) and 5,8-dimethoxypsoralen (isopimpinellin) ...201627434699
long-term boron-excess-induced alterations of gene profiles in roots of two citrus species differing in boron-tolerance revealed by cdna-aflp.boron (b) toxicity is observed in some citrus orchards in china. however, limited data are available on the molecular mechanisms of citrus b-toxicity and b-tolerance. using cdna-aflp, we identified 20 up- and 52 down-regulated genes, and 44 up- and 66 down-regulated genes from excess b-treated citrus sinensis and citrus grandis roots, respectively, thereby demonstrating that gene expression profiles were more affected in the latter. in addition, phosphorus and total soluble protein concentration ...201627446128
citrus functional genomics and molecular modeling in relation to citrus sinensis (sweet orange) infection with xylella fastidiosa (citrus variegated chlorosis).citrus are among the economically most important fruit tree crops in the world. citrus variegated chlorosis (cvc), caused by xylella fastidiosa infection, is a serious disease limiting citrus production at a global scale. with availability of citrus genomic resources, it is now possible to compare citrus expressed sequence tag (est) data sets and identify single-nucleotide polymorphisms (snps) within and among different citrus cultivars that can be exploited for citrus resistance to infections, ...201627447755
enhanced acquisition rates of 'candidatus liberibacter asiaticus' by the asian citrus psyllid (hemiptera: liviidae) in the presence of vegetative flush growth in citrus.the asian citrus psyllid preferentially feeds and exclusively reproduces on young, newly emerged flush shoots of citrus. asian citrus psyllid nymphs feed and complete their life stages on these flush shoots. recent studies conducted under greenhouse conditions have shown that the transmission rates of 'candidatus liberibacter asiaticus' (clas), the putative causal agent of huanglongbing disease of citrus, are enhanced when flush shoots are present. however, it is unclear if clas acquisition by m ...201627451998
aluminum toxicity-induced alterations of leaf proteome in two citrus species differing in aluminum tolerance.seedlings of aluminum-tolerant 'xuegan' (citrus sinensis) and al-intolerant 'sour pummelo' (citrus grandis) were fertigated for 18 weeks with nutrient solution containing 0 and 1.2 mm alcl₃·6h₂o. al toxicity-induced inhibition of photosynthesis and the decrease of total soluble protein only occurred in c. grandis leaves, demonstrating that c. sinensis had higher al tolerance than c. grandis. using isobaric tags for relative and absolute quantification (itraq), we obtained more al toxicity-respon ...201627455238
rapid discrimination of bergamot essential oil by paper spray mass spectrometry and chemometric analysis.a novel approach for the rapid discrimination of bergamot essential oil from other citrus fruits oils is presented. the method was developed using paper spray mass spectrometry (ps-ms) allowing for a rapid molecular profiling coupled with a statistic tool for a precise and reliable discrimination between the bergamot complex matrix and other similar matrices, commonly used for its reconstitution. ambient mass spectrometry possesses the ability to record mass spectra of ordinary samples, in their ...201627460885
proteomic analysis highlights the role of detoxification pathways in increased tolerance to huanglongbing disease.huanglongbing (hlb) disease is still the greatest threat to citriculture worldwide. although there is not any resistance source in the citrus germplasm, a certain level of moderated tolerance is present. a large-scale analysis of proteomic responses of citrus may help: 1) clarifying physiological and molecular effects of disease progression, 2) validating previous data at transcriptomic level, and 3) identifying biomarkers for development of early diagnostics, short-term therapeutics and long-te ...201627465111
evaluation of the spatiotemporal dynamics of oxytetracycline and its control effect against citrus huanglongbing via trunk injection.citrus huanglongbing (hlb) or greening is a devastating bacterial disease that has destroyed millions of trees and is associated with phloem-residing 'candidatus liberibacter asiaticus' (las) in florida. in this study, we evaluated the spatiotemporal dynamics of oxytetracycline in planta and its control effect against hlb via trunk injection. las-infected 'hamlin' sweet orange trees on 'swingle' citrumelo rootstock at the early stage of decline were treated with oxytetracycline hydrochloride (ot ...201627482624
identification and transcript profiles of citrus growth-regulating factor genes involved in the regulation of leaf and fruit development.growth-regulating factor (grf) is an important protein in ga-mediated response, with key roles in plant growth and development. however, it is not known whether or how the grf proteins in citrus to regulate organ size. in this study, nine citrus grf genes (csgrf1-9) were validated from the 'anliu' sweet orange (al, citrus sinensis cv. anliu) by pcr amplification. they all contain two conserved motifs (qlq and wrc) and have 3-4 exons. the transcript levels of genes were detected by qrt-pcr. trans ...201627491940
rapid and accurate identification of xanthomonas citri subspecies citri by fluorescence in situ hybridization.citrus canker is an economically important disease caused by the bacterial pathogen xanthomonas citri subsp. citri (xcc). this organism targets a wide range of citrus plants, including sweet orange, grapefruit, lemon and lime. as xcc is spread by environmental factors such as wind and rain, it is difficult to control its movement once the disease has established. in order to facilitate monitoring of citrus canker we sought to design a novel diagnostic protocol based on fluorescence in situ hybri ...201627492577
comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (citrus sinensis).citrate, the predominant organic acid in citrus, determines the taste of these fruits. however, little is known about the synergic molecular processes regulating citrate accumulation. using 'dahongtiancheng' (citrus sinensis) and 'bingtangcheng' (c. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'dahongtiancheng' and 'bingtangcheng' exhibit significantly different patterns in ci ...201627507765
lrr-rlk family from two citrus species: genome-wide identification and evolutionary aspects.leucine-rich repeat receptor-like kinases (lrr-rlks) represent the largest subfamily of plant rlks. the functions of most lrr-rlks have remained undiscovered, and a few that have been experimentally characterized have been shown to have important roles in growth and development as well as in defense responses. although rlk subfamilies have been previously studied in many plants, no comprehensive study has been performed on this gene family in citrus species, which have high economic importance a ...201627515968
deficit irrigation and maturation stage influence quality and flavonoid composition of 'valencia' orange fruit.effects of continuous deficit irrigation (di) and partial rootzone drying (prd) treatments (50% etc) in comparison with full irrigation (ci, 100% etc) were investigated during 'valencia' orange fruit maturation. ultra-high-performance liquid chromatography/high-resolution mass spectrometry was used to quantify hesperidin, narirutin, tangeritin, nobiletin, didymin and neoeriocitrin in the fruit juice and peel.201727528197
treatment of textile industry effluents using orange waste: a proposal to reduce color and chemical oxygen demand.various agricultural residues have been tested as biosorbents due to their low cost, high surface area, and favorable surface chemistry. in this work, a sweet orange albedo was tested as a biosorbent for treatment of real textile effluents. the orange albedo powder was prepared by drying the residue at 50 °c and milling to 30 mesh, and then used for dye adsorption from a alkaline (ph = 10.71) effluent. the adsorption process was studied in batch experiments at 30 °c by measuring color removal an ...201727533873
cspao4 of citrus sinensis functions in polyamine terminal catabolism and inhibits plant growth under salt stress.polyamine oxidase (pao) is a key enzyme catalyzing polyamine catabolism leading to h2o2 production. we previously demonstrated that citrus sinensis contains six putative pao genes, but their functions are not well understood. in this work, we reported functional elucidation of cspao4 in polyamine catabolism and salt stress response. cspao4 was localized to the apoplast and used both spermidine (spd) and spermine (spm) as substrates for terminal catabolism. transgenic plants overexpressing cspao4 ...201627535697
essential oil components of orange peels and antimicrobial activity.in this study, the orange peel of 12 cultivars of citrus sinensis from central-eastern sicily was employed to obtain essential oils and extracts. the ones were extracted through steam distillation, the others through extraction in hexane. chemical constituents were evaluated in terms of qualitative and quantitative analyses by gas chromatography/mass spectrometry. fifty-four components were identified in the steam essential oils and 44 in the extracts. in all the cultivars, the main component is ...201727535797
bioactive compounds in blood oranges (citrus sinensis (l.) osbeck): level and intake.both the composition and the intake of antioxidants (anthocyanins, ascorbic acid and hydroxycinnamic acids) were reported for all blood oranges including the single cultivars (moro, tarocco and sanguinello) and industrially produced juices. the mean values of the studied bioactive compounds in the edible part oranges were: 9.6mg/100g of orange edible part for the anthocyanins; 8.1, 0.7, 1.3, 3.8, 2.5mg/100g for total hydroxycinnamic acids, caffeic, sinapic, ferulic and coumaric acids, respective ...201727542451
fungal planet description sheets: 400-468.novel species of fungi described in the present study include the following from australia: vermiculariopsiella eucalypti, mulderomyces natalis (incl. mulderomyces gen. nov.), fusicladium paraamoenum, neotrimmatostroma paraexcentricum, and pseudophloeospora eucalyptorum on leaves of eucalyptus spp., anungitea grevilleae (on leaves of grevillea sp.), pyrenochaeta acaciae (on leaves of acacia sp.), and brunneocarpos banksiae (incl. brunneocarpos gen. nov.) on cones of banksia attenuata. novel foli ...201627616795
anti-proliferative or cytopathic potential of thapsia garganica, citrus sinensis, citrus limon and vinca rosea extracts against human embryonic kidney carcinoma cell line.developing anti-cancer drugs from natural products is receiving increasing interest worldwide due to limitations and side-effects of anti-cancer drugs. the purpose of this study was to explore the anti-proliferative or cytopathic potential of natural compounds derived from plant sources as alternatives of synthetic compounds on human embryonic kidney carcinoma (hek) cell line.201627618522
yield, esterification degree and molecular weight evaluation of pectins isolated from orange and grapefruit peels under different conditions.orange (citrus sinensis) and grapefruit (citrus paradise) peels were used as a source of pectin, which was extracted under different conditions. the peels are used under two states: fresh and residual (after essential oil extraction). organic acid (citric acid) and mineral acid (sulfuric acid) were used in the pectin extraction. the aim of this study is the evaluation the effect of extraction conditions on pectin yield, degree of esterification "de" and on molecular weight "mw". results showed t ...201627644093
the anticonvulsant activity of a flavonoid-rich extract from orange juice involves both nmda and gaba-benzodiazepine receptor complexes.the usage of dietary supplements and other natural products to treat neurological diseases has been growing over time, and accumulating evidence suggests that flavonoids possess anticonvulsant properties. the aim of this study was to examine the effects of a flavonoid-rich extract from orange juice (oje) in some rodent models of epilepsy and to explore its possible mechanism of action. the genetically audiogenic seizures (ags)-susceptible dba/2 mouse, the pentylenetetrazole (ptz)-induced seizure ...201627657037
impact of d-limonene synthase up- or down-regulation on sweet orange fruit and juice odor perception.citrus fruits are characterized by a complex mixture of volatiles making up their characteristic aromas, being the d-limonene the most abundant one. however, its role on citrus fruit and juice odor is controversial. transgenic oranges engineered for alterations in the presence or concentration of few related chemical groups enable asking precise questions about their contribution to overall odor, either positive or negative, as perceived by the human nose. here, either down- or up-regulation of ...201727664619
the effect of inhalation of citrus sinensis flowers and mentha spicata leave essential oils on lung function and exercise performance: a quasi-experimental uncontrolled before-and-after study.recently, there has been an increased interest in the effects of essential oils on athletic performances and other physiological effects. this study aimed to assess the effects of citrus sinensis flower and mentha spicata leaves essential oils inhalation in two different groups of athlete male students on their exercise performance and lung function.201727688737
principal component analysis (pca) of volatile terpene compounds dataset emitted by genetically modified sweet orange fruits and juices in which a d-limonene synthase was either up- or down-regulated vs. empty vector controls.we have categorized the dataset from content and emission of terpene volatiles of peel and juice in both navelina and pineapple sweet orange cultivars in which d-limonene was either up- (s), down-regulated (as) or non-altered (ev; control) ("impact of d-limonene synthase up- or down-regulation on sweet orange fruit and juice odor perception"(a. rodríguez, j.e. peris, a. redondo, t. shimada, e. costell, i. carbonell, c. rojas, l. peña, (2016)) [1]). data from volatile identification and quantific ...201627689126
comparative analysis of mirnas and their target transcripts between a spontaneous late-ripening sweet orange mutant and its wild-type using small rna and degradome sequencing.fruit ripening in citrus is not well-understood at the molecular level. knowledge of the regulatory mechanism of citrus fruit ripening at the post-transcriptional level in particular is lacking. here, we comparatively analyzed the mirnas and their target genes in a spontaneous late-ripening mutant, "fengwan" sweet orange (mt) (citrus sinensis l. osbeck), and its wild-type counterpart ("fengjie 72-1," wt). using high-throughput sequencing of small rnas and rna degradome tags, we identified 107 kn ...201627708662
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