Publications

influence of crop load on the expression patterns of starch metabolism genes in alternate-bearing citrus trees.	the fruit is the main sink organ in citrus and captures almost all available photoassimilates during its development. consequently, carbohydrate partitioning and starch content depend on the crop load of citrus trees. nevertheless, little is known about the mechanisms controlling the starch metabolism at the tree level in relation to presence of fruit. the aim of this study was to find the relation between the seasonal variation of expression and activity of the genes involved in carbon metaboli ...	2014	24747724
characterization of the glutathione s-transferase gene family through ests and expression analyses within common and pigmented cultivars of citrus sinensis (l.) osbeck.	glutathione s-transferases (gsts) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. anthocyanin pigments require gsts for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. anthocyanin accumulation in citrus sinensis (l.) osbeck fruit flesh determines different phenotypes affecting the typical pigmentation of sicilian blood oranges. in th ...	2014	24490620
citrus sinensis annotation project (cap): a comprehensive database for sweet orange genome.	citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. sweet orange accounts for more than half of the citrus production both in fresh fruit and processed juice. we have sequenced the draft genome of a double-haploid sweet orange (c. sinensis cv. valencia), and constructed the citrus sinensis annotation project (cap) to store and visualize the sequenced genomic and transcriptome data. cap provides gbrowse-bas ...	2014	24489955
determination of allergenic hydroperoxides in essential oils using gas chromatography with electron ionization mass spectrometry.	fragrance monoterpenes are widely used commercially due to their pleasant scent. in previous studies, we have shown that air-exposed monoterpenes form hydroperoxides that are strong skin sensitizers. methods for detection and quantification of the hydroperoxides in essential oils and scented products are thus desirable. due to thermolability and low uv absorbance, this is a complicated task. we have recently developed a sensitive lc-esi-ms method, but with limited structural information and sepa ...	2014	24482420
comparative de novo transcriptome analysis and metabolic pathway studies of citrus paradisi flavedo from naive stage to ripened stage.	grapefruit (citrus pardisi) is a popular citrus fruit that is a cross between a sweet orange and pummelo. this research article focuses on an in silico approach for comparative analysis of c. paradisi green flavedo (gf) and ethylene treated flavedo (etf) transcriptome data. our pathway analysis provides comprehensive information of genes playing significant role in different stages of ripening in fruit. de novo assembly was carried out using six different assemblers namely gs assembler, seqman n ...	2014	24477585
population of endogenous pararetrovirus genomes in carrizo citrange.	the complete genome sequences of three related endogenous pararetroviruses (eprvs) were obtained by 454 sequencing of nucleic acid extracts from carrizo citrange, used as a citrus rootstock. numerous homologous sequences have been found in the sweet orange genome. the new eprvs are most closely related to petunia vein-clearing virus.	2014	24435856
effect of sweet orange fruit waste diets and acidifier on haematology and serum chemistry of weanling rabbits.	a total of thirty-five mixed breed (35) rabbits of average weight of 700 g aged 5-6 weeks were allocated to seven treatments in a completely randomised design to investigate the effect of sweet orange fruit waste (sofw) and acidomix acidifier on haematology and serum chemistry. the diets were 0% sofw, 10% sofw with 0.5% acidomix, 10% sofw with 0.7 acidomix, 15% sofw with 0.5% acidifier, 15% sofw with 0.7% acidifier, 20% sofw with 0.5% acidifier, and 20% sofw with 0.7% acidifier. blood samples we ...	2014	26464931
first evidence of c- and o-glycosyl flavone in blood orange (citrus sinensis (l.) osbeck) juice and their influence on antioxidant properties.	rp-lc-dad-esi-ms-ms separation/identification protocol has been employed for the identification and characterisation of nine c- and o-glycosyl flavonoids in moro (citrus sinensis (l.) osbeck) juice grown in southern italy. for the first time we reported the presence of five c-glycosyl flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin), a 3-hydroxy-3-methylglutaryl glycosyl flavonol (3-hydroxy-3-methylglutaryl glycosyl quercetin) and a flavone o-glycosides (chrys ...	2014	24295703
genomewide analysis of small rnas in nonembryogenic and embryogenic tissues of citrus: microrna- and sirna-mediated transcript cleavage involved in somatic embryogenesis.	somatic embryogenesis (se) is a process of somatic cells becoming dedifferentiated and generating embryos. se has been widely used in biotechnology as a powerful way of regeneration and a model system for studying plant embryogenesis, but the controlling mechanisms of se are far from clear. here, we show the genomewide profiles of mirnas/sirnas and their target genes in nonembryogenic and embryogenic tissues of 'valencia' sweet orange. by high-throughput sequencing (hts) of small rnas and rna de ...	2015	25615015
clinical evaluation of moro (citrus sinensis (l.) osbeck) orange juice supplementation for the weight management.	in the last years, several studies have recently evaluated the beneficial effects of red orange juice (citrus sinensis (l.) osbeck) and its active components in weight management and obesity. moro orange is a cultivar of red orange, particularly rich in active compounds such as anthocyanins, hydroxycinnamic acids, flavone glycosides and ascorbic acid, which displays anti-obesity effects in in vitro and in vivo studies. in this clinical study, the effect of a moro juice extract (morosil(®), 400 m ...	2015	25588369
in vitro bioaccessibility of carotenoids, flavonoids, and vitamin c from differently processed oranges and orange juices [citrus sinensis (l.) osbeck].	carotenoid, flavonoid, and vitamin c concentrations were determined in fresh orange segments and a puree-like homogenate derived thereof, as well as freshly squeezed, flash-pasteurized, and pasteurized juices. lutein and β-cryptoxanthin were slightly degraded during dejuicing, whereas β-carotene levels were retained. vitamin c levels remained unaffected, whereas flavonoid levels decreased 8-fold upon juice extraction, most likely due to the removal of flavonoid-rich albedo and juice vesicles. li ...	2015	25539394
development and characterization of activated hydrochars from orange peels as potential adsorbents for emerging organic contaminants.	activated hydrochars obtained from the hydrothermal carbonization of orange peels (citrus sinensis) followed by various thermochemical processing were assessed as adsorbents for emerging contaminants in water. thermal activation under flows of co2 or air as well as chemical activation with phosphoric acid were applied to the hydrochars. their characteristics were analyzed and related to their ability to uptake three pharmaceuticals (diclofenac sodium, salicylic acid and flurbiprofen) considered ...	2015	25742754
investigation on the effect of different levels of dried sweet orange (citrus sinensis) pulp on performance, carcass characteristics and physiological and biochemical parameters in broiler chicken.	utilization of agricultural by-products in animal nutrition is a matter of great concern. dried sweet orange (citrus sinensis) pulp (dcsp) is a potential source of valuable nutrients and natural antioxidants for poultry feed. in the experiment, a feeding trial was conducted in order to investigate the effect of different levels of dried orange residues in diet on broiler growth performance, carcass characteristics, blood metabolites, humoral immunity, and cecum microbial population. a total of 2 ...	2015	25737644
correction to in vitro bioaccessibility of carotenoids, flavonoids, and vitamin c from differently processed oranges and orange juices [citrus sinensis (l.) osbeck].		2015	25714679
carbon isotope ratios of selected volatiles in citrus sinensis and in orange-flavoured food.	twenty genuine samples of industrially cold-pressed sweet orange essential oils, were analysed by gas chromatography-combustion-isotope ratio mass spectrometry to determine the values of the carbon isotope ratios (δ(13)c(vpdb)) of selected volatiles and assess the corresponding range of authenticity. successively, four commercial orange-flavoured products were analysed under identical conditions to evaluate the authenticity of the orange flavour. the samples were extracted by solid-phase microex ...	2015	25475589
degradation kinetic modelling of ascorbic acid and colour intensity in pasteurised blood orange juice during storage.	the stability of ascorbic acid and colour intensity in pasteurised blood orange juice (citrus sinensis [l.] osbeck) during one month of storage was investigated at 4-37 °c. the effects of ascorbic acid fortification (at 100, 200 mg l(-1)) and deaeration, temperature/time storage on the kinetic behaviour were determined. ascorbic acid was monitored by hplc-dad and colour intensity by spectrophotometric measurements. degradation kinetics were best fitted by first-order reaction models for both asc ...	2015	25466074
different citrus rootstocks present high dissimilarities in their antioxidant activity and vitamins content according to the ripening stage.	"lane late" sweet orange grafted on six different citrus rootstocks and grown in the guadalquivir valley (seville, spain) were picked at different ripening stages in two consecutive seasons to characterize their antioxidant activity (free radicals scavenging activity, reducing power and lipid peroxidation inhibition) and quantify their main antioxidant compounds (vitamin e and vitamin c). linear discriminant analysis and 2-way anova were applied to compare the effects induced by citrus rootstock ...	2015	25462974
incidence of 'candidatus liberibacter asiaticus'-infected plants among citrandarins as rootstock and scion under field conditions.	huanglongbing (hlb), caused by the bacterium 'candidatus liberibacter' spp., is currently one of the most serious diseases of citrus plants and has caused substantial economic losses. thus far, there is no source of genetic resistance to hlb in the genus citrus or its relatives. however, several studies have reported poncirus trifoliata and some of its hybrids to be more tolerant to the disease. the main objective of this study was to report differences in the incidence of 'ca. l. asiaticus' inf ...	2015	25423067
mirnaome analysis associated with anatomic and transcriptomic investigations reveal the polar exhibition of corky split vein in boron deficient citrus sinensis.	corky split vein can develop under long-term boron deficient conditions in citrus sinensis l. osbeck cv. newhall. this symptom only occurs in the upper rather than the lower epidermis of old leaves. our previous study demonstrated that vascular hypertrophy was involved in the symptoms, and the 3rd developmental stage of corky split vein (bd3) was the critical stage for phenotype formation. here, we performed an intensive study on the bd3 vein and its control sample (ck3 vein). a lignin test demo ...	2015	25754997
proteomic and metabolomic profiling of valencia orange fruit after natural frost exposure.	the aim of this study was to evaluate the response of orange fruit (citrus sinensis var. valencia late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among ...	2015	25132553
uptake and distribution of soil applied zinc by citrus trees-addressing fertilizer use efficiency with 68zn labeling.	the zinc (zn) supply increases the fruit yield of citrus trees that are grown, especially in the highly weathered soils of the tropics due to the inherently low nutrient availability in the soil solution. leaf sprays containing micronutrients are commonly applied to orchards, even though the nutrient supply via soil could be of practical value. this study aimed to evaluate the effect of zn fertilizers that are applied to the soil surface on absorption and partitioning of the nutrient by citrus t ...	2015	25751056
isolation and characterization of carotenoid cleavage dioxygenase 4 genes from different citrus species.	in plants, the carotenoid cleavage dioxygenase 4 (ccd4) could target on plastoglobules and cleave specific carotenoids, producing apocarotenoids and volatile compounds. these compounds are important for color and aroma formation in fruits and flowers. in this study, five ccd4 gene members (ccd4a, b, c, d, and e) were investigated in different citrus species including mandarin, pummelo, and sweet orange. sequence analysis showed that the ccd4 genes from all the species examined exhibited extensiv ...	2015	25749981
assessment of navel oranges, clementine tangerines, and rutaceous fruits as hosts of bactrocera cucurbitae and bactrocera latifrons (diptera: tephritidae).	export of citrus spp. fruits may require risk mitigation measures if grown in areas with established tephritid fruit fly (diptera: tephritidae) populations capable of infesting the fruits. the host status of citrus spp. fruits is unclear for two tephritid fruit fly species whose geographic ranges have expanded in recent years: melon fly, bactrocera cucurbitae (cocquillett), and bactrocera latifrons (hendel). in no choice cage infestation studies, b. latifrons oviposited into intact and punctured ...	2015	26816484
effect of blue led light intensity on carotenoid accumulation in citrus juice sacs.	in the present study, the effects of blue led light intensity on carotenoid accumulation and expression of genes related to carotenoid biosynthesis were investigated in the juice sacs of satsuma mandarin (citrus unshiu marc.) and valencia orange (citrus sinensis osbeck) in vitro. the results showed that 100 μmol m(-2)s(-1) blue led light (100b) was effective for increasing carotenoid content, especially β-cryptoxanthin, in satsuma mandarin after cultured in vitro for four weeks. in valencia oran ...	2015	26432407
application of sensory evaluation, hs-spme gc-ms, e-nose, and e-tongue for quality detection in citrus fruits.	in this study, electronic tongue (e-tongue), headspace solid-phase microextraction gas chromatography-mass spectrometer (gc-ms), electronic nose (e-nose), and quantitative describe analysis (qda) were applied to describe the 2 types of citrus fruits (satsuma mandarins [citrus unshiu marc.] and sweet oranges [citrus sinensis {l.} osbeck]) and their mixing juices systematically and comprehensively. as some aroma components or some flavor molecules interacted with the whole juice matrix, the change ...	2015	26416698
selection of reference genes for expression analyses of red-fleshed sweet orange (citrus sinensis).	red-fleshed oranges (citrus sinensis) contain high levels of carotenoids and lycopene. the growing consumer demand for products with health benefits has increased interest in these types of citrus cultivars as a potential source of nutraceuticals. however, little is known about the physiology of these cultivars under brazilian conditions. transcriptome and gene expression analyses are important tools in the breeding and management of red-fleshed sweet orange cultivars. reverse transcription quan ...	2015	26782492
long-term boron-deficiency-responsive genes revealed by cdna-aflp differ between citrus sinensis roots and leaves.	seedlings of citrus sinensis (l.) osbeck were supplied with boron (b)-deficient (without h3bo3) or -sufficient (10 μm h3bo3) nutrient solution for 15 weeks. we identified 54 (38) and 38 (45) up (down)-regulated cdna-aflp bands (transcript-derived fragments, tdfs) from b-deficient leaves and roots, respectively. these tdfs were mainly involved in protein and amino acid metabolism, carbohydrate and energy metabolism, nucleic acid metabolism, cell transport, signal transduction, and stress response ...	2015	26284101
a comprehensive analysis of chromoplast differentiation reveals complex protein changes associated with plastoglobule biogenesis and remodeling of protein systems in sweet orange flesh.	globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (citrus sinensis) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. subsequent isobaric tag for relative and absolute quantitation (itraq)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,38 ...	2015	26056088
structure and mechanism of dimer-monomer transition of a plant poly(a)-binding protein upon rna interaction: insights into its poly(a) tail assembly.	poly(a)-binding proteins (pabps) play crucial roles in mrna biogenesis, stability, transport and translational control in most eukaryotic cells. although animal pabps are well-studied proteins, the biological role, three-dimensional structure and rna-binding mode of plant pabps remain largely uncharacterized. here, we report the structural features and rna-binding mode of a citrus sinensis pabp (cspabpn1). cspabpn1 has a domain architecture of nuclear pabps (pabpns) with a single rna recognition ...	2015	26013164
the state of the art in biosynthesis of anthocyanins and its regulation in pigmented sweet oranges [(citrus sinensis) l. osbeck].	anthocyanins are water-soluble pigments belonging to the flavonoid compound family involved in nature in several aspects of plant development and defense. by bestowing much of the color and flavor on fruits and vegetables, they are components of the human diet and, thanks to their radical-scavenging properties, are not considered exclusively as food products but also as therapeutic agents. several cultivars of red (or blood) oranges [citrus sinensis (l.) osbeck], such as tarocco, moro, and sangu ...	2015	25871434
genome-wide identification, isolation and expression analysis of auxin response factor (arf) gene family in sweet orange (citrus sinensis).	auxin response factors (arfs) are an important family of proteins in auxin-mediated response, with key roles in various physiological and biochemical processes. to date, a genome-wide overview of the arf gene family in citrus was not available. a systematic analysis of this gene family in citrus was begun by carrying out a genome-wide search for the homologs of arfs. a total of 19 nonredundant arf genes (ciarf) were found and validated from the sweet orange. a comprehensive overview of the ciarf ...	2015	25870601
ultra-high performance liquid chromatography (uhplc) method for the determination of limonene in sweet orange (citrus sinensis) oil: implications for limonene stability.	the citrus-derived bioactive monoterpene limonene is an important industrial commodity and fragrance constituent. an rp isocratic elution c18 ultra-hplc (uhplc) method using a superficially porous stationary phase and photodiode array (pda) detector has been developed for determining the limonene content of sweet orange (citrus sinensis) oil. the method is fast with a cycle time of 1.2 min, linear, precise, accurate, specific, and stability indicating, and it satisfies u.s. pharmacopeia suitabil ...	2015	25857884
alternation of secondary metabolites and quality attributes in valencia orange fruit ( citrus sinensis ) as influenced by storage period and edible covers.	flavonoids (fgs) are a large group of polyphenolic compounds with low molecular weight, found in free and glycozidic forms in plants. citrus fruits can be used as a food supplement containing hesperidin and flavonoids to prevent infections and boost the immune system in human body. the aim of this study was the investigation of the effect of clove oil and storage period on the amount of hesperidin and naringin component in orange peel (cv. valencia). four treatments including clove oil (1 %), wa ...	2015	25829574
genome-wide identification, classification and analysis of hd-zip gene family in citrus, and its potential roles in somatic embryogenesis regulation.	the homeodomain-leucine zipper (hd-zip) transcription factors, which belong to a class of homeobox proteins, has been reported to be involved in different biological processes of plants, including growth and development, photomorphogenesis, flowering, fruit ripening and adaptation responses to environmental stresses. in this study, 27 hd-zip genes (cshbs) were identified in citrus. based on the phylogenetic analysis and characteristics of individual gene or protein, the hd-zip gene family in cit ...	2015	26232336
analysis of cuticular wax constituents and genes that contribute to the formation of 'glossy newhall', a spontaneous bud mutant from the wild-type 'newhall' navel orange.	navel orange (citrus sinensis [l.] osbeck) fruit surfaces contain substantial quantities of cuticular waxes, which have important eco-physiological roles, such as water retention and pathogen defense. the wax constituents of ripe navel orange have been studied in various reports, while the wax changes occurring during fruit development and the molecular mechanism underlying their biosynthesis/export have not been investigated. recently, we reported a spontaneous bud mutant from the wild-type (wt ...	2015	26177912
changes in the composition of aromatherapeutic citrus oils during evaporation.	the composition of some commercial citrus oils, lemon, sweet orange, and tangerine, designated for aromatherapy, was examined before and after partial evaporation in a stream of nitrogen. the intact oils contained the expected mixtures of mono- and sesquiterpenes, with hydrocarbons dominating and lesser amounts of oxygenated analogues making up the remainder. gas chromatography-mass spectrometry was used to follow alterations in the relative amounts of the various components present as evaporati ...	2015	26161120
bioavailability of β-cryptoxanthin is greater from pasteurized orange juice than from fresh oranges - a randomized cross-over study.	orange fruits and products thereof represent important dietary sources of carotenoids, particularly β-cryptoxanthin. since previous studies reported a positive effect of vegetable processing on carotenoid absorption, our objective was to compare the bioavailability of β-cryptoxanthin from either fresh navel oranges (citrus sinensis l. osbeck) or pasteurized orange juice.	2015	26114420
carotenoid bioaccessibility in pulp and fresh juice from carotenoid-rich sweet oranges and mandarins.	citrus fruits are a good source of carotenoids for the human diet; however, comparative studies of carotenoids in different citrus food matrices are scarce. in this work the concentration and bioaccessibility of carotenoids in sweet oranges and mandarins with marked differences in carotenoid composition were evaluated in pulp and compared to those in fresh juice. the pulp and juice of the red-fleshed cara cara sweet orange variety was highly rich in carotenes (mainly lycopene and phytoene) compa ...	2015	25996796
comparison of microwave, ultrasound and accelerated-assisted solvent extraction for recovery of polyphenols from citrus sinensis peels.	peel of citrus sinensis contains significant amounts of bioactive polyphenols that could be used as ingredients for a number of value-added products with health benefits. extraction of polyphenols from the peels was performed using a microwave-assisted extraction (mae) technique. the effects of aqueous acetone concentration, microwave power, extraction time and solvent-to-solid ratio on the total phenolic content (tpc), total antioxidant activity (taa) (using dpph and orac-values) and individual ...	2015	25977057
variety and harvesting season effects on antioxidant activity and vitamins content of citrus sinensis macfad.	five sweet orange (citrus sinensis osbeck) varieties cultivated in huelva (spain) and picked at two seasons during two consecutive years, were characterized for their antioxidant activity (free radicals scavenging activity, reducing power and lipid peroxidation inhibition) and vitamin content (vitamin e and vitamin c). the effects induced by sweet orange variety and stage of maturity were comprehensively compared by applying 2-way anova and linear discriminant analysis. the results indicated hig ...	2015	25961166
characterisation of free and bound volatile compounds from six different varieties of citrus fruits.	free volatile compounds in six varieties of citrus juices were analyzed by solid-phase microextraction-gas chromatography-mass spectrometry. bound fractions were isolated and extracted with methanol and amberlite xad-2 resin and then hydrolyzed by almond β-glucosidase. a total of 43 free and 17 bound volatile compounds were identified in citrus. free volatile contents in sweet orange were the most abundant, followed by those in grapefruits and mandarins. among free volatiles, terpenes were the m ...	2015	25952837
profiling gene expression in citrus fruit calyx abscission zone (az-c) treated with ethylene.	on-tree storage and harvesting of mature fruit account for a large proportion of cost in the production of citrus, and a reduction of the cost would not be achieved without a thorough understanding of the mechani sm of the mature fruit abscission. genome-wide gene expression changes in ethylene-treated fruit calyx abscission zone (az-c) of citrus sinensis cv. olinda were therefore investigated using a citrus genome array representing up to 33,879 citrus transcripts. in total, 1313 and 1044 diffe ...	2015	25948248
an investigation of boron-toxicity in leaves of two citrus species differing in boron-tolerance using comparative proteomics.	limited data are available on boron (b)-toxicity-responsive proteins in plants. we first applied 2-dimensional electrophoresis (2-de) to compare the effects of b-toxicity on leaf protein profiles in b-tolerant citrus sinensis and b-intolerant citrus grandis seedlings, and identified 27 (20) protein species with increased abundances and 23 (25) protein species with decreased abundances from the former (latter). generally speaking, b-toxicity increased the abundances of protein species involved in ...	2015	25892131
new insights into the evolutionary history of plant sorbitol dehydrogenase.	sorbitol dehydrogenase (sdh, ec 1.1.1.14) is the key enzyme involved in sorbitol metabolism in higher plants. sdh genes in some rosaceae species could be divided into two groups. l-idonate-5-dehydrogenase (lidh, ec 1.1.1.264) is involved in tartaric acid (ta) synthesis in vitis vinifera and is highly homologous to plant sdhs. despite efforts to understand the biological functions of plant sdh, the evolutionary history of plant sdh genes and their phylogenetic relationship with the v. vinifera li ...	2015	25879735
comparative study of flavonoid production in lycopene-accumulated and blonde-flesh sweet oranges (citrus sinensis) during fruit development.	four main flavanone glycosides (fgs) and four main polymethoxylated flavones (pmfs) were determined in fruits of 'cara cara' navel orange, 'seike' navel orange, 'anliu' and 'honganliu' sweet orange (citrus sinensis). no bitter neohesperidosides were detected in the fg profiles, indicating the functional inability of 1,2-rhamnosyltransferase, though relatively high transcription levels were detected in the fruit tissues of 'anliu' and 'honganliu' sweet oranges. different to the fgs, the pmfs only ...	2015	25872450
tobacco plants over-expressing the sweet orange tau glutathione transferases (csgstus) acquire tolerance to the diphenyl ether herbicide fluorodifen and to salt and drought stresses.	the glutathione transferases (gsts) are members of a superfamily of enzymes with pivotal role in the detoxification of both xenobiotic and endogenous compounds. in this work, the generation and characterization of transgenic tobacco plants over-expressing tau glutathione transferases from citrus sinensis (csgstu1 and csgstu2) and several cross-mutate forms of these genes are reported. putative transformed plants were verified for the presence of the transgenes and the relative quantification of ...	2015	25819876
genome-wide comparative analysis reveals similar types of nbs genes in hybrid citrus sinensis genome and original citrus clementine genome and provides new insights into non-tir nbs genes.	in this study, we identified and compared nucleotide-binding site (nbs) domain-containing genes from three citrus genomes (c. clementina, c. sinensis from usa and c. sinensis from china). phylogenetic analysis of all citrus nbs genes across these three genomes revealed that there are three approximately evenly numbered groups: one group contains the toll-interleukin receptor (tir) domain and two different non-tir groups in which most of proteins contain the coiled coil (cc) domain. motif analysi ...	2015	25811466
rare earth element transfer from soil to navel orange pulp (citrus sinensis osbeck cv. newhall) and the effects on internal fruit quality.	the effects of soil rare earth element (ree) on navel orange quality and safety in rare earth ore areas have gained great attention. this study investigated the transfer characteristics of ree from soil to navel orange pulp (citrus sinensis osbeck cv. newhall) and examined the effects of soil ree on internal fruit quality in xinfeng county, jiangxi province, china. path analysis showed that soil ree, ph, cation exchange capacity (cec), and fe oxide (feox) significantly affected pulp ree concentr ...	2015	25806821
two-dimensional gel electrophoresis data in support of leaf comparative proteomics of two citrus species differing in boron-tolerance.	here, we provide the data from a comparative proteomics approach used to investigate the response of boron (b)-tolerant 'xuegan' (citrus sinensis) and b-intolerant 'sour pummelo' (citrus grandis) leaves to b-toxicity. using two-dimensional gel electrophoresis (2-de) technique, we identified 50 and 45 protein species with a fold change of more than 1.5 and a p-value of less than 0.05 from b-toxic c. sinensis and c. grandis leaves. these b-toxicity-responsive protein species were mainly involved i ...	2015	26217760
living on the edges: spatial niche occupation of asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), in citrus groves.	the spatial niche occupation of the asian citrus psyllid, diaphorina citri kuwayama, 1908, was evaluated to determine its field colonization and food resource exploitation strategies in citrus groves. mature grapefruit and sweet orange groves were surveyed as part of an area-wide program in 2009-2010 to determine d. citri population densities and between-tree distribution. in both cultivars, significantly more psyllids were found on perimeter trees throughout the study period suggesting a strong ...	2015	26193111
hplc-dad phenolic characterization and antioxidant activities of ripe and unripe sweet orange peels.	phenolic compounds of unripe and ripe sweet orange peels were determined using a high-performance liquid chromatography separation method with diode array detector (hplc-dad). the in vitro antioxidant properties and the ec50 (concentration required to obtain a 50% antioxidant effect) values were also determined. the predominant phenolic compounds were quercitrin, rutin, and quercetin with values of 18.77 ± 0.01 mg/ml, 18.65 ± 0.03 mg/ml, and 10.39 ± 0.01 mg/ml respectively in unripe orange peel ...	2015	26783839
identification, characterization and expression analysis of lineage-specific genes within sweet orange (citrus sinensis).	with the availability of rapidly increasing number of genome and transcriptome sequences, lineage-specific genes (lsgs) can be identified and characterized. like other conserved functional genes, lsgs play important roles in biological evolution and functions.	2015	26597278
root itraq protein profile analysis of two citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity.	limited information is available on aluminum (al)-toxicity-responsive proteins in woody plant roots. seedlings of 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) were treated for 18 weeks with nutrient solution containing 0 (control) or 1.2 mm alcl3 · 6h2o (+al). thereafter, we investigated citrus root protein profiles using isobaric tags for relative and absolute quantification (itraq). the aims of this work were to determine the molecular mechanisms of plants to deal with al-tox ...	2015	26573913
boron-deficiency-responsive micrornas and their targets in citrus sinensis leaves.	micrornas play important roles in the adaptive responses of plants to nutrient deficiencies. most research, however, has focused on nitrogen (n), phosphorus (p), sulfur (s), copper (cu) and iron (fe) deficiencies, limited data are available on the differential expression of mirnas and their target genes in response to deficiencies of other nutrient elements. in this study, we identified the known and novel mirnas as well as the boron (b)-deficiency-responsive mirnas from citrus leaves in order t ...	2015	26538180
[prediction of cadmium content in the leaves of navel orange in heavy metal contaminated soil using vis-nir reflectance spectroscopy].	visual and near-infrared (vis-nir) reflectance spectroscopy had been used widely in monitoring agricultural pollution in recent years, however, it was rarely applied in monitoring the contamination of heavy metal in orchards. in the present paper, newhall navel orange (citrus sinensis [l.] osbeck cv. newhall) were cultivated in the potted soil contaminated with cadmium (cd) at different levels, and the spectral reflectance and cd content in the leaves were measured simultaneously at different gr ...	2015	26978924
red-fleshed sweet orange juice improves the risk factors for metabolic syndrome.	orange juice consumption can promote lower levels of oxidative stress and inflammation due to the antioxidant activity of citrus flavonoids and carotenoids. in addition, red-fleshed sweet orange juice (red orange juice) also contains lycopene. this study investigated the effects of red orange juice consumption on risk factors for metabolic syndrome. volunteers consumed red orange juice daily for 8 weeks, with clinical and biochemical assessments performed at baseline and on the final day. there ...	2015	26471075
chemopreventive actions of blond and red-fleshed sweet orange juice on the loucy leukemia cell line.	red-fleshed sweet orange juice (roj) comes from a new variety of citrus cultivated in brazil that contains high levels of β-carotene and lycopene, and similar amounts of hesperidin (hsp) and nutrients, equivalently to blond orange juice (boj). such bioactive compounds are associated with chemopreventive actions in several cancer cell lines. the purpose of this study was to examine the cytotoxicity, cell cycle, apoptosis, and cytokine secretion after boj, roj, and hsp treatment of a novel t acute ...	2015	26434864
gc-ms analysis of headspace and liquid extracts for metabolomic differentiation of citrus huanglongbing and zinc deficiency in leaves of 'valencia' sweet orange from commercial groves.	citrus huanglongbing (hlb) is considered the most destructive citrus disease worldwide. symptoms-based detection of hlb is difficult due to similarities with zinc deficiency.	2015	21046688
de novo transcriptome assembly of pummelo and molecular marker development.	pummelo (citrus grandis) is an important fruit crop worldwide because of its nutritional value. to accelerate the pummelo breeding program, it is essential to obtain extensive genetic information and develop relative molecular markers. here, we obtained a 12-gb transcriptome dataset of pummelo through a mixture of rna from seven tissues using illumina pair-end sequencing, assembled into 57,212 unigenes with an average length of 1010 bp. the annotation and classification results showed that a tot ...	2015	25799271
estimation of transgene copy number in transformed citrus plants by quantitative multiplex real-time pcr.	quantitative real-time pcr (qrt-pcr) was adapted to estimate transgene copy number of the rice xa21 gene in transgenic citrus plants. this system used taqman qrt-pcr and the endogenous citrus gene encoding for lipid transfer protein (ltp). transgenic "hamlin" sweet orange plants were generated using two different protoplast-gfp transformation systems: cotransformation and single plasmid transformation. a dilution series of genomic dna from one of the transgenic lines was used to generate a stand ...	2015	19194937
metabolic profiling reveals altered pattern of central metabolism in navel orange plants as a result of boron deficiency.	we focused on the changes of metabolite profiles in navel orange plants under long-term boron (b) deficiency using a gas chromatography-mass spectrometry (gc-ms) approach. curling of the leaves and leaf chlorosis were observed only in the upper leaves (present before start of the treatment) of b-deficient plants, while the lower leaves (grown during treatment) did not show any visible symptoms. the metabolites with up-accumulation in b-deficient leaves were mainly proline, l-ornithine, lysine, g ...	2015	25212059
citrus transformation using juvenile tissue explants.	the most frequently used method for production of citrus transgenic plants is via agrobacterium-mediated transformation of tissues found on explants obtained from juvenile seedlings. within the last decade and especially within the last 5-6 years, this robust method was employed to produce thousands of transgenic plants. with the newly applied screening methods that allow easier and faster detection of transgenic shoots, estimates of transformation rate for some cultivars have gone up making thi ...	2015	25416263
population dynamics of chaff scale, parlatoria pergandii comstock in comparative analysis of fluctuating population densities on three citrus varieties in el-beheira governorate, egypt.	investigation throughout the subsequent years of 2011-2012 and 2012-2013 indicated that chaff scale, p. pegandii is an important economic pest on citrus trees in abou el-matamer area, el-beheira governorate, egypt. it has been recorded in relatively high rates all over the year on three citrus varieties called succari, baladi and navel oranges. field observation and recorded data revealed that the common abundance was recorded for chaff scale on succari oranges followed by navel oranges then the ...	2015	27145572
phospholipids and terpenes modulate caco-2 transport of açaí anthocyanins.	anthocyanins (anc) are common polyphenolics in plants, but are poorly absorbed into the bloodstream upon consumption. phospholipids (pl) and terpenes (tp) may serve as enhancing agents in absorption. this study evaluated their role in transepithelial transport within a caco-2 cell monolayer-model system and impact on anc stability. açaí fruit anc were isolated and found to transport, at a low rate (1.22%), in the absence of soy lecithin phospholipids and valencia orange terpenes, yet their addit ...	2015	25577079
[effect of characteristic variable extraction on accuracy of cu in navel orange peel by libs].	heavy metals pollution in foodstuffs is more and more serious. it is impossible to satisfy the modern agricultural development by conventional chemical analysis. laser induced breakdown spectroscopy (libs) is an emerging technology with the characteristic of rapid and nondestructive detection. but libs' s repeatability, sensitivity and accuracy has much room to improve. in this work, heavy metal cu in gannan navel orange which is the jiangxi specialty fruit will be predicted by libs. firstly, th ...	2015	26717771
hesperetin attenuates ventilator-induced acute lung injury through inhibition of nf-κb-mediated inflammation.	hesperetin, a major bioflavonoid in sweet oranges and lemons, has been reported to have anti-inflammatory properties. however, the effect of hesperetin on ventilator-induced acute lung injury has not been studied. in present study, we investigated the protective effect of hesperetin on ventilator-induced acute lung injury in rats. rats were orally administered hesperetin (10, 20, or 40mg/kg) two hour before acute lung injury was induced by mechanical ventilation. rats were then randomly divided ...	2015	26610718
heavy metals in navel orange orchards of xinfeng county and their transfer from soils to navel oranges.	this study investigated heavy metal concentrations in soils and navel oranges of xinfeng county, a well-known navel orange producing area of china. the results showed that the average concentrations of lead (pb), cadmium (cd), chromium (cr), arsenic (as) and mercury (hg) in orchard soils all increased compared to the regional background values, especially for cd, which increased by 422%. when compared to the chinese environmental quality standard for soil (gb15618-1995), pb, cr and hg concentrat ...	2015	26232042
exploring the diversity in citrus fruit colouration to decipher the relationship between plastid ultrastructure and carotenoid composition.	differentiation of new and characteristic plastid ultrastructures during ripening of citrus fruits in both peel and pulp appears to be strongly correlated with the content and complement of carotenoids. most of the species of the citrus genus display a wide range in fruit colouration due to differences in carotenoids; however, how this diversity is related and may contribute to plastid differentiation and ultrastructure is currently unknown. to that end, carotenoid profile and plastid ultrastruc ...	2015	26202736
content evaluation of 4 furanocoumarin monomers in various citrus germplasms.	due to the furanocoumarin compounds in the fruit, the production and consumption of grapefruit have been affected in the past decades since the 'grapefruit juice effect' was declared. to provide elite germplasm and obtain knowledge for future citrus breeding programs, the contents of 4 furanocoumarin monomers (fcms) in the juice sacs from 73 citrus germplasms were evaluated using ultra-performance liquid chromatography. 6',7'-dihydroxybergamottin and bergamottin were dominant in all the tested g ...	2015	25977000
nuclear species-diagnostic snp markers mined from 454 amplicon sequencing reveal admixture genomic structure of modern citrus varieties.	most cultivated citrus species originated from interspecific hybridisation between four ancestral taxa (c. reticulata, c. maxima, c. medica, and c. micrantha) with limited further interspecific recombination due to vegetative propagation. this evolution resulted in admixture genomes with frequent interspecific heterozygosity. moreover, a major part of the phenotypic diversity of edible citrus results from the initial differentiation between these taxa. deciphering the phylogenomic structure of c ...	2015	25973611
regulation of ascorbic acid metabolism by blue led light irradiation in citrus juice sacs.	in the present study, the effects of red and blue led lights on the accumulation of ascorbic acid (asa) were investigated in the juice sacs of three citrus varieties, satsuma mandarin, valencia orange, and lisbon lemon. the results showed that the blue led light treatment effectively increased the asa content in the juice sacs of the three citrus varieties, whereas the red led light treatment did not. by increasing the blue led light intensity, the juice sacs of the three citrus varieties accumu ...	2015	25711821
differences in antennal sensillae of male and female peach fruit flies in relation to hosts.	antennal sensillae of male and female peach fruit flies, bactrocera zonata (saunders) (diptera: tephritidae), obtained from three different host fruit species (guava, psidium guajava l. (myrtales: myrtaceae); peach, prunus persica (l.) stokes (rosales: rosaceae); and orange, citrus sinensis (l.) osbeck (sapindales: rutaceae)), were studied with scanning electron microscopy. this study was carried out to describe the different types of sensillae present on the three antennal segments (scape, pedi ...	2015	25688086
gc-fid/ms profiling of supercritical co2 extracts of peels from citrus aurantium, c. sinensis cv. washington navel, c. sinensis cv. tarocco and c. sinensis cv. doppio sanguigno from dubrovnik area (croatia).	the peels of citrus aurantium l. and citrus sinensis osbeck cultivars from the dubrovnik region (south croatia) were extracted by supercritical co2 at 40 degrees c and 10 mpa at 1.76 kg/h to obtain enriched extracts in comparison with simple pressing of the peels. the extracts were analyzed in detail by gas chromatography and mass spectrometry (gc-fid/ms). relevant similarities among the peel oil compositions of c. aurantium and c. sinensis cultivars were found with limonene predominance (up to ...	2015	26411039
comprehensive analyses of genomes, transcriptomes and metabolites of neem tree.	neem (azadirachta indica a. juss) is one of the most versatile tropical evergreen tree species known in india since the vedic period (1500 bc-600 bc). neem tree is a rich source of limonoids, having a wide spectrum of activity against insect pests and microbial pathogens. complex tetranortriterpenoids such as azadirachtin, salanin and nimbin are the major active principles isolated from neem seed. absolutely nothing is known about the biochemical pathways of these metabolites in neem tree. to id ...	2015	26290780
genome-wide identification and expression analysis of the polyamine oxidase gene family in sweet orange (citrus sinensis).	polyamine oxidases (paos) are fad-dependent enzymes associated with polyamine catabolism. in plants, increasing evidences support that pao genes play essential roles in abiotic and biotic stresses response. in this study, six putative pao genes (cspao1-cspao6) were unraveled in sweet orange (citrus sinensis) using the released citrus genome sequences. a total of 203 putative cis-regulatory elements involved in hormone and stress response were predicted in 1.5-kb promoter regions at the upstream ...	2015	25445392
a systematic review of the anxiolytic-like effects of essential oils in animal models.	the clinical efficacy of standardized essential oils (such as lavender officinalis), in treating anxiety disorders strongly suggests that these natural products are an important candidate source for new anxiolytic drugs. a systematic review of essential oils, their bioactive constituents, and anxiolytic-like activity is conducted. the essential oil with the best profile is lavendula angustifolia, which has already been tested in controlled clinical trials with positive results. citrus aurantium ...	2015	26473822
late embryogenesis abundant (lea) constitutes a large and diverse family of proteins involved in development and abiotic stress responses in sweet orange (citrus sinensis l. osb.).	late embryogenesis abundant (lea) proteins are an ubiquitous group of polypeptides that were first described to accumulate during plant seed dehydration, at the later stages of embryogenesis. since then they have also been recorded in vegetative plant tissues experiencing water limitation and in anhydrobiotic bacteria and invertebrates and, thereby, correlated with the acquisition of desiccation tolerance. this study provides the first comprehensive study about the lea gene family in sweet orang ...	2015	26700652
the effect of citrus-derived oil on bovine blood neutrophil function and gene expression in vitro.	research on the use of natural products to treat or prevent microbial invasion as alternatives to antibiotic use is growing. polymorphonuclear leukocytes (pmnl) play a vital role with regard to the innate immune response that affects severity or duration of mastitis. to our knowledge, effect of cold-pressed terpeneless valencia orange oil (tco) on bovine pmnl function has not been elucidated. therefore, the objective of this study was to investigate the effect of tco on bovine blood pmnl chemota ...	2015	25434342
quantification and localization of hesperidin and rutin in citrus sinensis grafted on c. limonia after xylella fastidiosa infection by hplc-uv and maldi imaging mass spectrometry.	a high performance liquid chromatography-ultraviolet (hplc-uv) method was developed for quantifying hesperidin and rutin levels in leaves and stems of citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg ml(-1) respectively, with r(2)>0.999 for all curves. the limits of detection (lod) for both flavonoids were 0.6 and 0.5 μg ml(-1), respectively, with quantification (loq) being 2.0 and 1.0 μg ml(-1), respectively. the quantification method was applied to citrus sinensis ...	2015	25749617
genome-wide characterization and expression analysis of major intrinsic proteins during abiotic and biotic stresses in sweet orange (citrus sinensis l. osb.).	the family of aquaporins (aqps), or major intrinsic proteins (mips), includes integral membrane proteins that function as transmembrane channels for water and other small molecules of physiological significance. mips are classified into five subfamilies in higher plants, including plasma membrane (pips), tonoplast (tips), nod26-like (nips), small basic (sips) and unclassified x (xips) intrinsic proteins. this study reports a genome-wide survey of mip encoding genes in sweet orange (citrus sinens ...	2015	26397813
development and reproduction of panonychus citri (prostigmata: tetranychidae) on different species and varieties of citrus plants.	the species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite panonychus citri (mcgregor) (prostigmata: tetranychidae) and alter the pest management programs in citrus groves. in this study we evaluated, in the laboratory, the development and reproduction of p. citri and estimated its life table parameters when reared on four varieties of citrus sinensis (l.) osbeck (valencia, pera, natal, and hamlin), one variety of citrus reticulata ...	2015	26459376
diversity in sensory quality and determining factors influencing mandarin flavor liking.	we evaluated the sensory quality of 42 mandarin varieties that belong to 7 different natural subgroups: common mandarin (citrus reticulata blanco), clementine (citrus clementina hort. ex. tan), satsuma (citrus unshiu marcovitch), mediterranean mandarin (citrus deliciosa tenore), king mandarin (citrus nobilis loureiro), and mandarin hybrids, such as tangor (citrus reticulata × citrus sinensis) and tangelo (citrus reticulata × citrus paradisi). consumer flavor acceptance tests revealed wide divers ...	2015	25559381
inhibition of metalloproteinase and proteasome activities in colon cancer cells by citrus peel extracts.	citrus peels are consumed in the form of infusions, candy or wine, based on their well-documented nutritional and medicinal properties. this study sought to investigate the effect of some citrus peels' [grapefruit (citrus paradisii), orange (citrus sinensis) and shaddock (citrus maxima)] extracts on matrix metalloproteinase (mmp) and proteasome activities in primary human colonic tumor (caco-2) and the metastatic cell lines (lovo and lovo/adr) in a bid to explain the possible mechanism by which ...	2015	26030791
house fly (musca domestica l.) attraction to insect honeydew.	house flies are of major concern as vectors of food-borne pathogens to food crops. house flies are common pests on cattle feedlots and dairies, where they develop in and feed on animal waste. by contacting animal waste, house flies can acquire human pathogenic bacteria such as escherichia coli and salmonella spp., in addition to other bacteria, viruses, or parasites that may infect humans and animals. the subsequent dispersal of house flies from animal facilities to nearby agricultural fields co ...	2015	25970333
bioactivity of six plant extracts on adults of demotispa neivai (coleoptera: chrysomelidae).	demotispa neivai bondar (coleoptera: chrysomelidae) damage oil palm fruits, which makes it necessary to develop products to control this insect. the mortality, repellency, and antifeeding effects on adults of d. neivai of six plant extracts of azadirachta indica a. juss. (sapindales: meliaceae), ricinus communis (l.) (malpighiaes: euphorbiaceae), citrus sinensis oesbek (sapindales: rutaceae), nicotiana tabacum (l.) (slanales: solanaceae), capsicum annuum (l.) (solanales: solanaceae), and artemis ...	2015	25843587
antimicrobial effects of citrus sinensis peel extracts against periodontopathic bacteria: an in vitro study.	use of plant extracts and phytochemicals with known antimicrobial properties may have great significance in therapeutic treatments.	2015	26024407
empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria.	in this study, fractional compound composition, antioxidant capacity, and phenolic substance content of 14 plant essential oils-anise (pimpinella anisum), bay leaves (laurus nobilis), cinnamon bark (cinnamomum verum), clove (eugenia caryophyllata), fennel (foeniculum vulgare), hop (humulus lupulus), istanbul oregano (origanum vulgare subsp. hirtum), izmir oregano (origanum onites), mint (mentha piperita), myrtus (myrtus communis), orange peel (citrus sinensis), sage (salvia officinalis), thyme ( ...	2015	25764982
antimicrobial nanoemulsion formulation with improved penetration of foliar spray through citrus leaf cuticles to control citrus huanglongbing.	huanglongbing (hlb) is the most serious disease affecting the citrus industry worldwide to date. the causal agent, candidatus liberibacter asiaticus (las), resides in citrus phloem, which makes it difficult to effectively treat with chemical compounds. in this study, a transcuticular nanoemulsion formulation was developed to enhance the permeation of an effective antimicrobial compound (ampicillin; amp) against hlb disease through the citrus cuticle into the phloem via a foliar spray. the result ...	2015	26207823
diversity and genetic variation among brevipalpus populations from brazil and mexico.	brevipalpus phoenicis s.l. is an economically important vector of the citrus leprosis virus-c (cilv-c), one of the most severe diseases attacking citrus orchards worldwide. effective control strategies for this mite should be designed based on basic information including its population structure, and particularly the factors that influence its dynamics. we sampled sweet orange orchards extensively in eight locations in brazil and 12 in mexico. population genetic structure and genetic variation b ...	2015	26207373
effect of different levels of dietary sweet orange (citrus sinensis) peel extract on humoral immune system responses in broiler chickens.	this experiment was conducted to evaluate the effects of different levels of sweet orange (citrus sinensis) peel extract (sope) on humoral immune system responses in broiler chickens. three hundred 1-day broilers (ross-308) were randomly allocated to treatments varying in supplemental sope added in the drinking water. the experimental groups consisted of three treatments fed for 42 days as follows: a control treatment without feed extract, a treatment containing 1000 ppm of sope and a treatment ...	2015	24990585
citrus tristeza virus (ctv) causing proteomic and enzymatic changes in sweet orange variety "westin".	citrus tristeza disease, caused by ctv (citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. the virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of tristeza. the objective of this study was to analyze the changes caused by ctv in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. the ctv-infected sweet orange displayed mil ...	2015	26207751
substrate specificity of plant and fungi pectin methylesterases: identification of novel inhibitors of pmes.	pectin methylesterases (pmes) play a central role in pectin remodeling during plant development. they are also present in phytopathogens such as bacteria and fungi. we investigated the substrate specificity and ph dependence of plant and fungi pmes using tailor-made pectic substrates. for this purpose, we used two plant pmes (from orange peel: citrus sinensis and from arabidopsis thaliana) and one fungal pme (from botrytis cinerea). we showed that plant and fungi pmes differed in their substrate ...	2015	26342461
transgenic citrus expressing an arabidopsis npr1 gene exhibit enhanced resistance against huanglongbing (hlb; citrus greening).	commercial sweet orange cultivars lack resistance to huanglongbing (hlb), a serious phloem limited bacterial disease that is usually fatal. in order to develop sustained disease resistance to hlb, transgenic sweet orange cultivars 'hamlin' and 'valencia' expressing an arabidopsis thaliana npr1 gene under the control of a constitutive camv 35s promoter or a phloem specific arabidopsis suc2 (atsuc2) promoter were produced. overexpression of atnpr1 resulted in trees with normal phenotypes that exhi ...	2015	26398891
construction of citrus gene coexpression networks from microarray data using random matrix theory.	after the sequencing of citrus genomes, gene function annotation is becoming a new challenge. gene coexpression analysis can be employed for function annotation using publicly available microarray data sets. in this study, 230 sweet orange (citrus sinensis) microarrays were used to construct seven coexpression networks, including one condition-independent and six condition-dependent (citrus canker, huanglongbing, leaves, flavedo, albedo, and flesh) networks. in total, these networks contain 37 6 ...	2015	26504573
genome-wide identification of sweet orange (citrus sinensis) histone modification gene families and their expression analysis during the fruit development and fruit-blue mold infection process.	in eukaryotes, histone acetylation and methylation have been known to be involved in regulating diverse developmental processes and plant defense. these histone modification events are controlled by a series of histone modification gene families. to date, there is no study regarding genome-wide characterization of histone modification related genes in citrus species. based on the two recent sequenced sweet orange genome databases, a total of 136 cshms (citrus sinensis histone modification genes) ...	2015	26300904
isolation and characterization of a novel pathogenesis-related protein gene (gmprp) with induced expression in soybean (glycine max) during infection with phytophthora sojae.	pathogenesis-related proteins (pr proteins) play crucial roles in the plant defense system. a novel prp gene was isolated from highly resistant soybean infected with phytophthora sojae (p. sojae) and was named gmprp (genbank accession number: km506762). the amino acid sequences of gmprp showed identities of 74%, 73%, 72% and 69% with prp proteins from vitis vinifera, populus trichocarpa, citrus sinensis and theobroma cacao, respectively. quantitative real-time reverse transcription pcr (qrt-pcr) ...	2015	26114301
the efficacy and underlying mechanism of sulfone derivatives containing 1,3,4-oxadiazole on citrus canker.	the objectives of the current study were to isolate and identify the pathogen responsible for citrus canker and investigate the efficacy of sulfone derivatives containing 1,3,4-oxadiazole moiety on controlling citrus canker caused by xanthomonas citri subsp. citri (xcc) under in vitro and field conditions. in an in vitro study, we tested eight sulfone derivatives against xcc and the results demonstrated that compound 3 exhibited the best antibacterial activity against xcc, with a half-maximal ef ...	2015	26247929
activity of six essential oils extracted from tunisian plants against legionella pneumophila.	the aim of this study was to investigate the composition of six essential oils extracted from tunisian plants, i.e., artemisia herba-alba asso, citrus sinensis (l.) osbeck, juniperus phoenicea l., rosmarinus officinalis l., ruta graveolens l., and thymus vulgaris l., and to evaluate their activity against legionella pneumophila (microdilution assays). eight legionella pneumophila strains were studied, including the two well-known serogroup 1 lens and paris strains as controls and six environment ...	2015	26460561
identification and molecular characterization of nuclear citrus leprosis virus, a member of the proposed dichorhavirus genus infecting multiple citrus species in mexico.	citrus leprosis is one of the most destructive diseases of citrus spp. and is associated with two unrelated virus groups that produce particles primarily in either the cytoplasm or nucleus of infected plant cells. symptoms of leprosis, including chlorotic spots surrounded by yellow haloes on leaves and necrotic spots on twigs and fruit, were observed on leprosis-affected mandarin and navel sweet orange trees in the state of querétaro, mexico. serological and molecular assays showed that the cyto ...	2015	25423071
deep sequencing and analysis of small rnas in sweet orange grafted on sour orange infected with two citrus tristeza virus isolates prevalent in sicily.	two representative isolates of a citrus tristeza virus population in sicily, sg29 (aggressive) and bau282 (mild), were sequenced via viral small rnas (vsrna) produced in budlings of sweet orange grafted on sour orange. phylogenetic relationships with mediterranean and exotic isolates revealed that sg29 clustered within the "vt-asian" subtype, whereas bau282 belonged to the cluster t30. the study confirms that molecular data need to be integrated with bio-indexing in order to obtain adequate info ...	2015	26175068