Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [visible transmittance techniques for rapid analysis of sugar content of fresh navel orange juices]. | the feasibility of visible spectroscopic technology for rapid quantifying sugar content (sc) of navel orange fresh juices was investigated by means of spectral transmittance technique. a total of 55 juice samples were used to develop the calibration and prediction models. calibration models based on different spectral ranges and different spectral pretreatment were compared in the precent research. performance of different models was investigated in terms of root mean square errors of prediction ... | 2007 | 17554924 |
| [effects of exogenous aba and ga3 on sugar concentration in flesh of cara cara navel orange]. | the concentration of glucose, fructose, sucrose and total sugar were determined after exogenous aba and ga3 treatment during young period of fruit and before fruit coloring in flesh of cara cara navel orange. the results showed that 10 mg x l(-1) aba treatment improved glucose, fructose and total sugar concentration significantly or very significantly, 50 mg x l(-1) aba treatment improved sucrose concentration very significantly, but 100 mg x l(-1) aba treatment reduced glucose concentration ver ... | 2007 | 18260446 |
| [near infrared diffuse reflectance spectroscopy for rapid analysis of soluble solids content in navel orange]. | the potential of using visible and near infrared diffuse reflectance spectroscopy to assess soluble solids content (ssc) of intact navel orange was examined. a total 40 samples were used to develop the calibration and prediction models. nir spectral data were collected in the spectral region between 350 and 2 500 nm and their second derivative spectra were used for the present study. different scattering correction algorithms (no preprocessing and multiplicative scattering correction (msc)) were ... | 2007 | 18260391 |
| the debittering of navel orange juice using polymeric films. | in order to better understand and optimize the sorption of limonin (the major navel orange juice bitter principle) by various plasticized polymeric films, a sorption and plasticizer migration study was carried out using high-performance liquid chromatography (hplc) and gas chromatography (gc). low molecular weight (lmw) poly (vinyl chloride) (pvc) plasticized with dioctyl adipate (doa) gave the best results for both limonin sorption and low doa migration. thick films did not significantly sorb m ... | 2007 | 17995766 |
| [construction and preliminary analysis of subtractive library for peel pitting of 'fengjie' navel orange fruit]. | in this study, the pitted peel and non-pitted peel of 'fengjie' navel orange fruits were used as experimental materials to construct and screen the peel pitting related genes by suppression subtractive hybridization (ssh). the results showed that suppression subtractive hybridization was very effective. a cdna library of differentially expressed genes was constructed. the library included about 200 clones with an average insert size of around 300 bp. part of the positive clones were picked up ra ... | 2007 | 17287572 |
| a screening method for the identification of glycosylated flavonoids and other phenolic compounds using a standard analytical approach for all plant materials. | a screening method was developed for the systematic identification of glycosylated flavonoids and other phenolic compounds in plant food materials based on an initial, standard analytical method. this approach applies the same analytical scheme (aqueous methanol extraction, reverse phase liquid chromatographic separation, and diode array and mass spectrometric detection) to every sample and standard. this standard approach allows the cross-comparison of compounds in samples, standards, and plant ... | 2007 | 17256956 |
| selection of colour of sticky trap for monitoring adult bean thrips, caliothrips fasciatus (thysanoptera: thripidae). | adult bean thrips, caliothrips fasciatus (pergande), overwintering inside the navel of navel oranges shipped from california to australia, are an actionable pest for the importing country, i.e. infested lots are fumigated with methyl bromide. strict quarantine regulations regarding c. fasciatus prompted studies on the best colour sticky trap that might be used to monitor for bean thrips populations in the vicinity of california citrus groves prior to harvesting fruit for export. preliminary expe ... | 2007 | 17125151 |
| a comparison of anion concentration in surficial groundwater sampled from two types of water quality monitoring wells. | groundwater sampling for monitoring the presence and concentration of contaminants can be done using either depth integrated monitoring wells (mw) or depth specific multi-level sampling (mls) wells. depth specific multi-level sampling wells (mls) are cost-effective, easy to install, and provide very detailed information about the vertical gradient in contaminant concentration. in contrast the mw sampling provides information on the presence of contaminant over large representative area. this stu ... | 2007 | 17129947 |
| a novel bud mutation that confers abnormal patterns of lycopene accumulation in sweet orange fruit (citrus sinensis l. osbeck). | a novel, pleiotropic sweet orange (citrus sinensis l. osbeck) mutant, 'hong anliu', is described. this mutation causes carotenoid accumulation, high sugar, and low acid in the fruits. gas chromatographic analysis revealed that high sugar and low acid in the fruit were caused by the accumulation of sucrose and the deficiency of citric acid. the dominant carotenoid accumulated in albedo, segment membranes, and juice sacs is lycopene, which can reach levels that are a 1000-fold higher than those in ... | 2007 | 18182424 |
| evolving window zone selection method followed by independent component analysis as useful chemometric tools to discriminate between grapefruit juice, orange juice and blends. | this study investigates the use of high resolution 1h nmr as a suitable alternative to the standard chromatographic method for the determination of adulteration of orange juice (citrus sinensis) with grapefruit juice (citrus paradisi) based on flavonoid glycoside content. fifty-nine orange juices (oj), 23 grapefruit juices (gj) and 10 blends (og), obtained from local retail outlets were used to assess the performance of the 1h nmr method. the work presented here introduces the evolving window zo ... | 2007 | 17683731 |
| molecular and immunological characterization of the glycosylated orange allergen cit s 1. | the ige of sera from patients with a history of allergy to oranges (citrus sinensis) binds a number of proteins in orange extract, including cit s 1, a germin-like protein. in the present study, we have analyzed its immunological cross-reactivity and its molecular nature. sera from many of the patients examined recognize a range of glycoproteins and neoglycoconjugates containing beta1,2-xylose and core alpha1,3-fucose on their n-glycans. these reagents also inhibited the interaction of cit s 1 w ... | 2007 | 17095532 |
| efficient and scalable method in isolation of polymethoxyflavones from orange peel extract by supercritical fluid chromatography. | polymethoxyflavones (pmfs) from citrus genus are of particular interest because of their broad spectrum of biological activities, such as anti-inflammatory, anti-carcinogenic, and anti-atherogenic properties. recently, the exploration into the beneficial health properties of pmfs in citrus fruits has dramatically increased. however, the supply of pure pmfs in the in vivo study is a limiting factor due to the difficulties in large-scale isolation of the interested pmfs. therefore, the development ... | 2007 | 17035106 |
| whole-tree level water balance and its implications on stomatal oscillations in orange trees [citrus sinensis (l.) osbeck] under natural climatic conditions. | sustained cyclic oscillations in stomatal conductance, leaf water potential, and sap flow were observed in young orange trees growing under natural conditions. the oscillations had an average period of approximately 70 min. water uptake by the roots and loss by the leaves was characterized by large time lags which led to imbalances between water supply and demand in the leaves. the bulk of the lag in response between stomatal movements and the upstream water balance resided downstream of the bra ... | 2007 | 17443016 |
| isolation and syntheses of polymethoxyflavones and hydroxylated polymethoxyflavones as inhibitors of hl-60 cell lines. | fifteen polymethoxyflavones (pmfs) and hydroxylated pmfs were isolated from sweet orange (citrus sinensis) peel extract and synthesized to investigate their biological activity. all obtained compounds were tested in hl-60 cancer cell proliferation and apoptosis induction assays. while some pmfs and hydroxylated pmfs had moderate anti-carcinogenic activities, 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone and 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone showed strong inhibitory activities against the pr ... | 2007 | 17391969 |
| binomial sampling to estimate rust mite (acari: eriophyidae) densities on orange fruit. | binomial sampling based on the proportion of samples infested was investigated for estimating mean densities of citrus rust mite, phyllocoptruta oleivora (ashmead), and aculops pelekassi (keifer) (acari: eriophyidae), on oranges, citrus sinensis (l.) osbeck. data for the investigation were obtained by counting the number of motile mites within 600 sample units (each unit a 1-cm2 surface area per fruit) across a 4-ha block of trees (32 blocks total): five areas per 4 ha, five trees per area, 12 f ... | 2007 | 17370833 |
| plant somatic hybrid cytoplasmic dna characterization by single-strand conformation polymorphism. | unlike maternal inheritance in sexual hybridization, plant somatic hybridization allows transfer, mixing and recombination of cytoplasmic genomes. in addition to the use of somatic hybridization in plant breeding programs, application of this unique tool should lead to a better understanding of the roles played by the chloroplastic and mitochondrial genomes in determining agronomically important traits. the nucleotide sequences of cytoplasmic genomes are much more conserved than those of nuclear ... | 2007 | 17331897 |
| [seasonal variation of immature stages of phyllocnistis citrella stainton (lepidoptera: gracillariidae) in citrus sinensis orchards under two management systems]. | phyllocnistis citrella stainton is considered an important pest of citrus, causing both direct (reduction on the photosynthetic area) and indirect damage (facilitation of invasion by bacteria that cause citrus canker). the lack of information about the population dynamics of p. citrella, considering the cultivation systems and varieties grown in citrus orchards in rio grande do sul, brazil, motivated this study. it aimed to evaluate the seasonal variation of immature stages of p. citrella, from ... | 2007 | 17273716 |
| [effects of foliage spraying sodium bisulfite on photosynthetic physiological characteristics of citrus sinensis with sulphur deficiency]. | the study with solution culture showed that citrus sinensis plants with sulphur deficiency and with sulphur deficiency plus foliage spraying sodium bisulfite ( nahso3 ) halfway had notably decreased photosynthetic pigment content, soluble protein content, net photosynthetic rate (pn), initial fluorescence (fo) , maximum fluorescence (fm) , photochemical efficiency (fv/fm) and electron transmit rate (etr) , compared with those having normal sulphur supply. after 35 days of growth, their photosynt ... | 2007 | 17450735 |
| expression of phytoene synthase gene (psy) is enhanced during fruit ripening of cara cara navel orange (citrus sinensis osbeck). | citrus is an important fruit crop as regards accumulation of carotenoids. in plant carotenoid biosynthesis, phytoene synthase gene (psy) plays a key role in catalyzing the head-to-head condensation of geranylgeranyl diphosphate molecules to produce colorless phytoene. in the present paper, we reported the phytoene contents determination and characterization of psy during fruit ripening of "washington" navel orange and its red-fleshed mutant "cara cara". results showed that phytoene was exclusive ... | 2007 | 17226057 |
| cire1, a novel transcriptionally active ty1-copia retrotransposon from citrus sinensis. | ltr retrotransposons (ltr-rtns) are widespread constituents of eukaryote genomes, particularly plant genomes. although ltr-rtns from plants were thought to be transcriptionally silent in somatic tissues, evidences of activity under certain conditions are available for some of them. in order to investigate ltr-rtns in the citrus sinensis genome, we analysed them by pcr using degenerate primers corresponding to highly conserved domains. all elements of the two types of ltr-rtn comprise about 23% o ... | 2007 | 17216224 |
| an expedient and versatile protocol for extracting high-quality dna from plant leaves. | introductionprotocols for extracting plant genomic dna have to contend with two major challenges: preventing the oxidation of phenolic substances that can react with nucleic acids and proteins, and eliminating polysaccharides that interfere with downstream enzymatic manipulations of dna. a protocol has been developed in which the above-mentioned substances are removed from the sample before the dna is removed from the chromatin structure. this protocol requires only two tubes during extraction a ... | 2007 | 21357104 |
| quality by design: understanding the formulation variables of a cyclosporine a self-nanoemulsified drug delivery systems by box-behnken design and desirability function. | quality by design (qbd) refers to the achievement of certain predictable quality with desired and predetermined specifications. a very useful component of the qbd is the understanding of factors and their interaction effects by a desired set of experiments. the present project deals with a case study to understand the effect of formulation variables of nanoemulsified particles of a model drug, cyclosporine a (cya). a three-factor, three-level design of experiment (doe) with response surface meth ... | 2007 | 17169518 |
| preference-performance linkage of the xylem feeding leafhopper, homalodisca vitripennis (hemiptera cicadellidae). | insect species exhibiting a weak linkage between adult preference and immature performance have frequently been shown to be prone to outbreaks. we used choice and no-choice tests to examine the preference-performance linkage of the xylem fluid-feeding leafhopper, homalodisca vitripennis germar. leafhoppers were offered a choice of hosts common to their native range and also a choice from hosts where they have been recently introduced. behavior (residence preference, oviposition preference, and c ... | 2007 | 18284780 |
| quality by design: characterization of self-nano-emulsified drug delivery systems (snedds) using ultrasonic resonator technology. | in the present work, a novel application of ultrasonic measurements is detailed to characterize nano-emulsion formulations as a part of the overall quality by design (qbd) goal. ultrasonic resonator technology (urt) was utilized to measure sound velocity and absorption of self-nanoemulsified drug delivery systems (snedds) consisting of various ratios of oil:surfactant:co-surfactant. a qbd concept was used to create different snedds formulations utilizing sweet orange oil (oil), emulphor-620 (sur ... | 2007 | 17521836 |
| quality by design: understanding the product variability of a self-nanoemulsified drug delivery system of cyclosporine a. | the objective of this work was to understand the product variability due to size and other characteristics of the snedds by utilizing near infrared (nir) and chemometric analysis, as well as several other well-known procedures. pseudo-ternary phase diagrams were constructed to identify the efficient self-emulsification region using cya solutions in sweet orange oil (oily phase), emulphor el-620 (surfactant), and capmul mcm-c8 (cosurfactant). the formulated snedds were characterized by droplet si ... | 2007 | 17628495 |
| ammonium transport and citamt1 expression are regulated by light and sucrose in citrus plants. | here the isolation and characterization of citamt1 cdna from citrange troyer (citrus sinensis l. osbeckxponcirus trifoliata blanco) is reported, suggesting that this belongs to the amt gene family, which is involved in the high-affinity transport system (hats). results show that in citrus plants, the hats is much more dependent on the light conditions and c status of the roots than the low-affinity transport system. most importantly, a strong correlation was found between the regulation of both ... | 2007 | 17615410 |
| [analysis of constituents of essential oils from fresh and dried pericarp of citrus sinensis by gc-ms]. | to analyze and compare the constituents of the essential oils from the fresh and dried pericarps of citrus sinensis. | 2007 | 17552156 |
| effect of foliar application of micronutrients on the yield and quality of sweet orange (citrus sinensis l.). | an experiment was designed to study the effect of foliar application of micronutrients on the yield, quality and leaf composition of sweet orange, blood red variety at shabazgari, mardan. the experiment was laid out in a randomized complete block design in 2) factorial arrangement. zinc, manganese and boron were applied as foliar spray at the rate of 0.4, 0.2 and 0.04 kg ha(-1), respectively in the presence of 1.56 kg n ha(-1) as urea and 0.4 kg surfactance ha(-1) (as wetting agent) in 400 l of ... | 2007 | 19086544 |
| hydraulic characteristics and water relations in pigment-less mutant shoots of an orange tree. | phenotypes more or less deficient in photosynthetic pigments show reduced productivity. not much is known, however, about the influence of pigment-less twigs on the water balance of whole trees. we studied the water relations and hydraulic properties of normal and pigment-less (white) and 1-year-old shoots of 12-year-old citrus sinensis l. trees. compared with green leaves, white leaves showed a pronounced deficiency of pigments, higher stomatal density, the absence of chloroplasts in the guard ... | 2007 | 17241963 |
| polyphenols distribution in juices from citrus allotetraploid somatic hybrids and their sexual hybrids. | the polyphenol profile of an interspecific allotetraploid somatic hybrid, achieved by protoplast fusion, the 'valencia' sweet orange ( citrus sinensis l. osbeck) + 'femminello' lemon ( citrus limon l. burm), and three sexual hybrids obtained by backcrosses between femminello lemon and the allotetraploid somatic hybrid ('valencia' + 'femminello') was studied by liquid chromatography-ultraviolet-diode array detector-mass spectrometry (lc-uv-dad-ms). the aim of the work was to evaluate whether supe ... | 2007 | 17914878 |
| behavioral responses of schistocerca americana (orthoptera: acrididae) to azadirex (neem)-treated host plants. | azadirex (azadirachtin and other biologically active extracts from neem trees) has been shown to have considerable potential to be used in integrated pest management systems based on its growth regulator/insecticide properties. less well known are the antifeedant properties. the feeding-deterrent properties of a commercial azadirex formulation (azatrol ec) were evaluated using both no-choice and choice tests, the american grasshopper, schistocerca americana (drury), and four host plants [savoy c ... | 2007 | 17370818 |
| efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination. | the presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. in citrus, selection using the selectable marker gene nptii, that confers resistance to the antibiotic kanamycin, is in general very effective. an attractive alternative is offered by the mat system (multi-auto-transformation), which combines the ipt gene for positive selection with the recombinase system r/rs for removal of ma ... | 2007 | 16927091 |
| effect of temperature on development and survival of the parasitoid pnigalio pectinicornis (hymenoptera: eulophidae) reared on phyllocnistis citrella (lepidoptera: gracillariidae). | laboratory studies were conducted to assess the effect of temperature on the development and survival of the indigenous parasitoid pnigalio pectinicornis l. on the citrus leaf miner phyllocnistis citrella stainton as host, fed on leaves of citrus sinensis l. osbck cultivar washington navel and citrus reticulata blanco cultivar clementine. experiments were conducted at five constant temperatures ranging from 15 to 32.5 degrees c, with 60 +/- 10% rh and a photoperiod of 14:10 (l:d) h. the relation ... | 2007 | 17540056 |
| evaluation of endophytic colonization of citrus sinensis and catharanthus roseus seedlings by endophytic bacteria. | over the last few years, the endophytic bacterial community associated with citrus has been studied as an important component interacting with xylella fastidiosa, the causal agent of citrus variegated chlorosis (cvc). this bacterium may also colonize some model plants, such as catharanthus roseus and nicotiana clevelandii. in the present study, we compared the endophytic colonization of citrus sinensis and catharanthus roseus using the endophytic bacteria klebsiella pneumoniae. we chose an appro ... | 2007 | 17342049 |
| effect of storage and processing on plasmid, yeast and plant genomic dna stability in juice from genetically modified oranges. | recombinant dna technology is an important tool in the development of plant varieties with new favourable features. there is strong opposition towards this technology due to the potential risk of horizontal gene transfer between genetically modified plant material and food-associated bacteria, especially if genes for antibiotic resistance are involved. since horizontal transfer efficiency depends on size and length of homologous sequences, we investigated the effect of conditions required for or ... | 2007 | 17064805 |
| the survival of three strains of arcobacter butzleri in the presence of lemon, orange and bergamot essential oils and their components in vitro and on food. | to test the effect of oils and vapours of lemon, sweet orange and bergamot and their components against three arcobacter butzleri strains. | 2007 | 17451515 |
| rapd-based genetic analysis of offsprings from the sexual cross using allotetraploid citrus somatic hybrid as pollen parent. | thirty-one polymorphic decamer primers were selected to genotype 92 progenies from the cross between yiben no.4, a monoembryonic diploid f1 hybrid of citrus reticulata blanco cv huanongbendizao tangerine and c. ichangensis swingle, and [hamlin sweet orange + rough lemon], an allotetraploid somatic hybrid of citrus sinensis osbeck cv. hamlin and c. jambhiri lush cv. rough lemon. chi2 (chi-square) analysis of rapd markers in the progenies indicated they were randomly transmitted from the four dono ... | 2007 | 17609894 |
| determination of polymethoxylated flavones in peels of selected jamaican and mexican citrus (citrus spp.) cultivars by high-performance liquid chromatography. | the concentrations of the polymethoxylated flavones (pmfs) in peels of selected citrus cultivars grown in jamaica and mexico were determined. the pmfs were extracted from sun-dried citrus peels with reagent-grade methanol. analyses were carried out by reverse-phase hplc and uv detection. the column used was a c(18) 5 microm (150 x 4.6 mm) discovery column. elution was in the gradient mode, using a ternary mobile phase. the results showed that all the citrus cultivars used contained at least thre ... | 2007 | 17080505 |
| a ptha homolog from xanthomonas axonopodis pv. citri responsible for host-specific suppression of virulence. | strains of the plant-pathogenic bacterium xanthomonas axonopodis pv. citri are differentiated into two groups with respect to aggressiveness (normal and weak) on citrus grandis cultivars but not on other citrus species such as citrus sinensis. random mutagenesis using the transposon tn5 in x. axonopodis pv. citri strain kc21, which showed weak aggressiveness on a c. grandis cultivar, was used to isolate mutant kc21t46, which regained a normal level of aggressiveness on the cultivar. the gene ina ... | 2007 | 17293422 |
| preferential accumulation of severe variants of citrus tristeza virus in plants co-inoculated with mild and severe variants. | the viral population in sweet orange plants, either healthy or pre-inoculated with the asymptomatic isolate of citrus tristeza virus (ctv) t32, and then graft- or aphid-inoculated with the stem-pitting isolate t318, was characterized with respect to symptom expression, reaction with monoclonal antibody mca13, single-strand conformation polymorphism (sscp) of genes p18 and p20, bi-directional rt-pcr, and dot-blot hybridisation. all plants inoculated with t318, with or without pre-inoculation, sho ... | 2007 | 17294089 |
| karyotype diversity and the origin of grapefruit. | grapefruit is a group of citrus of recent origin, probably resulting from a cross between pummelo and sweet-orange. aiming to investigate this putative origin and the genetic variability among grapefruit cultivars, the karyotype of six grapefruits, two pummelos, and one tangelo cultivar (grapefruit x tangerine) were analyzed using sequential cma/dapi double staining and fish with rdna probes. the karyotypes of grapefruit 'duncan' and 'foster' differ from those of 'flame', 'henderson', 'marsh' an ... | 2007 | 17295131 |
| the endophyte curtobacterium flaccumfaciens reduces symptoms caused by xylella fastidiosa in catharanthus roseus. | citrus variegated chlorosis (cvc) is a disease of the sweet orange [citrus sinensis (l.)], which is caused by xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. sweet orange trees have been occasionally observed to be infected by xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. the principal endophytic bacterial species isolated from such cv ... | 2007 | 17978797 |
| apoptosis-inducing activity of hydroxylated polymethoxyflavones and polymethoxyflavones from orange peel in human breast cancer cells. | sweet orange (citrus sinensis l.) peel is a rich resource of flavonoids, especially polymethoxyflavones (pmfs). citrus flavonoids exert a broad spectrum of biological activity, including antiproliferative and proapoptotic effects in cancer cells. we have recently shown that individual pmfs from orange peel induce ca(2+)-mediated apoptosis in human breast cancer cells and that hydroxylation of pmfs is critical for enhancing their proapoptotic activity. here, we report that the fraction of orange ... | 2007 | 17979096 |
| thermal degradation of antioxidant micronutrients in citrus juice: kinetics and newly formed compounds. | the thermal degradation kinetics of vitamin c, two carotenoids (beta-carotene and beta-cryptoxanthin), and hesperidin, as a function of temperature, were determined for citrus juice [citrus sinensis (l.) osbeck and citrus clementina hort. ex tan]. the influence of dissolved oxygen on the rate of ascorbic acid degradation was also assessed. analysis of kinetic data suggested a first-order reaction for the degradation of vitamin c and carotenoids. the kinetics parameters dtheta, z, and ea have bee ... | 2007 | 17451252 |
| structure and composition of the assemblage of parasitoids associated to phyllocnistis citrella pupae stainton (lepidoptera: gracillariidae) in citrus orchards in southern brazil. | the structure and composition of the assemblage of pupal parasitoids of phyllocnistis citrella stainton, the citrus leafminer, were studied in two citrus orchards (citrus deliciosa tenore cv. montenegrina and citrus sinensis (l.) osbeck x citrus reticulata blanco hybrid murcott), in montenegro county (29 degrees 68's and 51 degrees 46'w), southern brazil. at fortnightly samplings, from july 2001 to june 2003, all the new shoots from 24 randomly selected trees were inspected. the species richness ... | 2007 | 18060301 |
| induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets. | this study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of 'meyer' lemon. pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 gy). the resulting seeds were characterised by a small size. embryos were rescued in vitro and the ploidy level of the plantlets was determined by flow cytometry analysis. haploid, diploid, triploid plantlets were obtained. the hapl ... | 2007 | 17318461 |
| development of genetic maps of the citrus varieties 'murcott' tangor and 'pera' sweet orange by using fluorescent aflp markers. | the progeny of 87 bc(1) hybrids of 'murcott' tangor and 'pera' sweet orange, genotyped with fluorescent amplified fragment length polymorphism (faflp) markers, was used for the construction of genetic maps for both citrus varieties. mapping strategies, considering the progeny as a result of backcrossing and cross-pollination, were exploited in mapmaker 2.0 (lod score >or= 3.0 and <or= 0.40) and joinmap 3.0 software (lod score >or= 3.0 and theta <or = 0.25), respectively. genetic map distances (i ... | 2007 | 17666774 |
| etiology of three recent diseases of citrus in são paulo state: sudden death, variegated chlorosis and huanglongbing. | the state of são paulo (ssp) is the first sweet orange growing region in the world. yet, the ssp citrus industry has been, and still is, under constant attack from various diseases. in the 1940s, tristeza-quick decline (t-qd) was responsible for the death of 9 million trees in ssp. the causal agent was a new virus, citrus tristeza virus (ctv). the virus was efficiently spread by aphid vectors, and killed most of the trees grafted on sour orange rootstock. control of the disease resided in replac ... | 2007 | 17505974 |
| leaf water relations and net gas exchange responses of salinized carrizo citrange seedlings during drought stress and recovery. | since salinity and drought stress can occur together, an assessment was made of their interacting effects on leaf water relations, osmotic adjustment and net gas exchange in seedlings of the relatively chloride-sensitive carrizo citrange, citrus sinensis x poncirus trifoliata. | 2007 | 17575285 |
| cross-correlation patterns of air and soil temperatures, rainfall and diaprepes abbreviatus root weevil in citrus. | time series cross-correlation analysis is appropriate when measuring relationships between two different time series. using this approach, the authors quantified the relationship between the time series air temperature (at), soil temperature (st), rainfall, relative humidity (rh) and diaprepes abbreviatus (l.) (coleoptera: curculionidae) root weevil across a period of 30 months, and examined how closely the distribution of diaprepes root weevil was related to at, st, rainfall and rh within this ... | 2007 | 17674428 |
| argyrotaenia sphaleropa (meyrick) (lepidoptera: tortricidae) in citrus in the state of paraná, brazil. | argyrotaenia sphaleropa (meyrick) is associated with various fruit species in south america. this tortricid was first detected in citrus, citrus sinensis (l.) osbeck, in brazil, in commercial groves in northern paraná, during the 1994/95-crop season. the aim of this work was to study the biology of this lepidopteran under laboratory conditions (28 +/- 2 degrees c, 70 +/-10% ur, 14h photofase) using the citrus cultivar pêra. larvae obtained from egg masses collected in a commercial citrus grove i ... | 2007 | 17607470 |
| antidiabetic potential of citrus sinensis and punica granatum peel extracts in alloxan treated male mice. | an investigation on the effects of four different concentrations of peel extract from citrus sinensis (cs) or punica granatum (pg) in male mice revealed the maximum glucose lowering and antiperoxidative activities at 25 mg/kg of cs and 200 mg/kg of pg. in a separate experiment their potential was evaluated with respect to the regulation of alloxan induced diabetes mellitus. while a single dose of alloxan (120 mg/kg) increased the serum levels of glucose and alpha-amylase activity, rate of water ... | 2007 | 18806305 |
| functional characterization of ap3, soc1 and wus homologues from citrus (citrus sinensis). | flowering and flower formation are defining features of angiosperms and the control of these developmental processes involves a common repertoire of genes which are shared among different species of flowering plants. these genes were first identified using various homeotic and flowering time mutants of arabidopsis and snapdragon, and homologous genes have subsequently been isolated from a wide range of different plant species based on the conservation of protein sequence and function. using dege ... | 2007 | 18251886 |
| [population fluctuation of sharpshooters vectors of xylella fastidiosa wells et al. in commercial citrus groves in northwestern paraná state]. | the citrus variegated chlorosis (cvc), an important disease of citrus in brazil, is caused by the bacterium xylella fastidiosa wells et al. and transmitted by xylem-feeding sharpshooters (hemiptera: cicadellidae). this study evaluated the fluctuation of populations of species of sharpshooters belonging to the tribes cicadellini and proconiini, from subfamily cicadelinae, in a commercial sweet orange [citrus sinensis (l.) osb.] grove, located in the northwest region of paraná state, brazil, in fo ... | 2007 | 17607459 |
| chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated. | chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. to study the role and regulation of chlorophyllase (chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (citrus sinensis) chlase in two heterologous plant systems: (1) squash (cucurbita pepo) plants using a viral vector expression system; ... | 2007 | 17369368 |
| xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange citrus sinensis cv. pera. | xylella fastidiosa (xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (citrus sinensis l. osb.) causing pierce disease and citrus variegated chlorosis (cvc), respectively. the aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'pêra' (c. sinensis l. osbeck) infected with xf. plants were artificially infected and maintained in the greenhouse until they have de ... | 2007 | 17609534 |
| antiproliferative activity of citrus juices and hplc evaluation of their flavonoid composition. | the antiproliferative activity of fresh fruit juices extracted from citrus sinensis (cv. washington navel and cv. sanguinello), c. deliciosa cv. avana, c. clementina cv. nules, c. aurantium subsp. myrtifolia , was evaluated against k562 (human chronic myelogenous leukemia), hl-60 (human leukemia) and mcf-7 (human breast adenocarcinoma) cell lines. all the juices tested showed antiproliferative activity. moreover, the pattern of the main flavanone compounds in the juices has been determined by hp ... | 2007 | 17628348 |
| protein changes in the albedo of citrus fruits on postharvesting storage. | in this work, major protein changes in the albedo of the fruit peel of murcott tangor (tangerine x sweet orange) during postharvest ageing were studied through 2d page. protein content in matured on-tree fruits and in fruits stored in nonstressing [99% relative humidity (rh) and 25 degrees c], cold (99% rh and 4 degrees c), and drought (60% rh and 25 degrees c) conditions was initially determined. protein identification through ms/ms determinations revealed in all samples analyzed the occurrence ... | 2007 | 17910511 |
| common subtropical and tropical nonpollen food sources of the boll weevil (coleoptera: curculionidae). | it is known that substantial boll weevil, anthonomus grandis grandis boheman, individuals can survive mild subtropical winters in some habitats, such as citrus orchards. our study shows that endocarp of the fruit from prickly pear cactus, opuntia engelmannii salm-dyck ex. engel.; orange, citrus sinensis l. osbeck.; and grapefruit, citrus paradisi macfad., can sustain newly emerged adult boll weevils for >5 mo, which is the duration of the cotton-free season in the subtropical lower rio grande va ... | 2007 | 17349122 |
| difference of a citrus late-ripening mutant (citrus sinensis) from its parental line in sugar and acid metabolism at the fruit ripening stage. | 'fengjiewancheng' (fw) (citrus sinensis), a bud sport of 'fengjie 72-1' navel orange (fj), ripens one month later than its parental line. differences in sugar and acid content and the transcript level of sucrose-and citric-metabolic enzymes for the two cultivars were investigated during fruit ripening. results showed that both sugar and acid metabolisms of the mutant were affected by the mutation. in the pulp of fw, sugar content was significantly lower than that in fj before 227 daf (days after ... | 2007 | 17653673 |
| inhibitory effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone on 12-o-tetradecanoylphorbol 13-acetate-induced skin inflammation and tumor promotion in mice. | 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-oh-hxmf), a polymethoxyflavone, is found exclusively in the citrus genus, particularly in the peels of sweet orange. herein, we report the first investigation of the inhibitory effects of 5-oh-hxmf on 12-o-tetradecanoylphorbol-13-acetate (tpa)-induced expression of inducible nitric oxide synthase (inos) and cyclooxygenase-2 (cox-2) in mouse skin. we found that the topical application of 5-oh-hxmf can effectively inhibit the transcriptional activation ... | 2007 | 17962218 |
| modulation of p-glycoprotein activity by acridones and coumarins from citrus sinensis. | bioguided fractionation of the roots of citrus sinensis (rutaceae) led to the isolation and identification of five coumarins, namely, clausarin, suberosin, poncitrin, xanthyletin and thamnosmonin, seven acridones, namely, acrimarine b, 2-methoxycitpressine i, citpressine i, buntanine, acrimarine e, honyumine and acrimarine c, and one terpenoid, namely, limonin. among these compounds, clausarin, 2-methoxycitpressine i and acrimarine e inhibited p-glycoprotein-mediated drug efflux in k562/r7 human ... | 2007 | 17236173 |
| protection of citrus rootstocks against phytophthora spp. with a hypovirulent isolate of phytophthora nicotianae. | abstract phytophthora root rot of citrus in florida is caused by phytophthora nicotianae and p. palmivora. a naturally occurring isolate of p. nicotianae (pn117) was characterized as hypovirulent on citrus roots. pn117 infected and colonized fibrous roots, but caused significantly less disease than the virulent isolates p. nicotianae pn198 and p. palmivora pp99. coincident inoculation of rootstock seedlings of cleopatra mandarin (citrus reticulata) or swingle citrumelo (c. paradisi x poncirus tr ... | 2007 | 18943635 |
| genetic diversity of xylella fastidiosa strains from costa rica, são paulo, brazil, and united states. | abstract the diversity of 42 xylella fastidiosa strains from costa rica, são paulo, brazil, and the united states were analyzed using the sequence of the 16s rrna gene by variable number of tandem repeat (vntr) fragment analysis and by restriction fragment length polymorphisms (rflp) of a specific polymerase chain reaction (pcr)-amplification product using enzyme cfoi. limited variability in the sequence of the 16s rrna gene was observed and, although the separation was not absolute, most strain ... | 2007 | 18943693 |
| light, scanning electron microscopy and sds-page studies on the effect of the essential oil, citrus sinensis var. balady on the embryonic development of camel tick hyalomma dromedarii (koch, 1818) (acari: ixodidae). | gc-mse analysis of the essential oil of fresh fruit peel of citrus sinensis var. balady recognized two main natural toxic compounds, limonene (83.28%) as hydrocarbon compound and linalool (3.97%) as oxygenated compound. therefore, the objective of this study was to evaluate its effect on different egg-ages of hyalomma dromedarii at four concentrations of 1:40, 1:30, 1:20 and 1:15 (oil : ethanol 95%) (v/v). the lc50 values were 1:56, 1:34, 1:41, 1:32, 1: 23, 1:23, 1:18, 1:14 and 1:11 for egg-ages ... | 2007 | 19069909 |
| [spatial distribution of oncometopiafacialis (signoret) (hemiptera: cicadellidae) in citrus orchard]. | the spatial distribution of oncometopia facialis (signoret) was studied in sweet orange to elaborate sampling plans and decision-making procedure for the control of this sharpshooter in the field. the samplings were carried out fortnightly in a grid design disposition of 100 points (plants) in citrus orchard, with yellow sticky traps. the average number of o. facialis ranged from 0.35 to 1.17 insects/trap, with the i index varying from 0.89 to 1.82. the fit tests to negative binomial, poisson di ... | 2006 | 17352074 |
| potential perchlorate exposure from citrus sp. irrigated with contaminated water. | citrus produced in the southwestern united states is often irrigated with perchlorate-contaminated water. this irrigation water includes colorado river water which is contaminated with perchlorate from a manufacturing plant previously located near the las vegas wash, and ground water from wells in riverside and san bernardino counties of california which are affected by a perchlorate plume associated with an aerospace facility once located near redlands, california. studies were conducted to eva ... | 2006 | 17723376 |
| the effect of lemon, orange and bergamot essential oils and their components on the survival of campylobacter jejuni, escherichia coli o157, listeria monocytogenes, bacillus cereus and staphylococcus aureus in vitro and in food systems. | to investigate the effectiveness of oils and vapours of lemon (citrus limon), sweet orange (citrus sinensis) and bergamot (citrus bergamia) and their components against a number of common foodborne pathogens. | 2006 | 17105553 |
| inheritance and heritability of resistance to citrus leprosis. | abstract the genetic inheritance of resistance to leprosis, the most important viral disease of citrus in brazil, was characterized through the phenotypic assessment of 143 hybrids resulting from crosses between tangor 'murcott' (citrus sinensis x c. reticulata) and sweet orange 'pêra' (c. sinensis), considered to be resistant and susceptible to the disease, respectively. all plants were grafted onto rangpur lime (c. limonia) and inoculated with citrus leprosis virus, cytoplasmic type through th ... | 2006 | 18943497 |
| an effective and low-cost culture medium for isolation and growth of xylella fastidiosa from citrus and coffee plants. | buffered charcoal-yeast extract medium (bcye) has been used for isolation of xylella fastidiosa from citrus (citrus sinensis) and coffee (coffea arabica) plants affected by citrus variegated chlorosis (cvc) and coffee leaf scorch (cls). bcye is composed of aces (2-[2-amino-2oxoethyl) amino]-ethanesulfonic acid) buffer, activated charcoal, yeast extract, l-cysteine, ferric pyrophosphate, and agar. aces buffer is costly and not always commercially available in brazil, and the l-cysteine and ferric ... | 2006 | 17111277 |
| diversity of flies (diptera: tephritidae and lonchaeidae) in organic citrus orchards in the vale do rio caí, rio grande do sul, southern brazil. | this study was carried out in the counties of montenegro and pareci novo located in the region of the vale do rio cai, rio grande do sul, southern brazil, aiming to determine the fruit fly species of tephritidae and lonchaeidae that occur in organic orchards of sweet orange [citrus sinensis (l.) osb.] cultivar céu, and murcott tangor (citrus reticulata blanco x c. sinensis), during the fruit ripening stages in 2003 and 2004. eight mcphail traps baited with integral grape juice diluted to 25% wer ... | 2006 | 17144140 |
| rapid, specific and quantitative assays for the detection of the endophytic bacterium methylobacterium mesophilicum in plants. | xylella fastidiosa is a xylem-limited bacterium that causes citrus variegated chlorosis disease in sweet orange. there is evidence that x. fastidiosa interacts with endophytic bacteria present in the xylem of sweet orange, and that these interactions, particularly with methylobacterium mesophilicum, may affect disease progress. however, these interactions cannot be evaluated in detail until efficient methods for detection and enumeration of these bacteria in planta are developed. we have previou ... | 2006 | 16266765 |
| comparison of supercritical fluid chromatography and liquid chromatography for the separation of urinary metabolites of nobiletin with chiral and non-chiral stationary phases. | nobiletin (nob), a polymethoxylated flavone found in sweet orange (citrus sinensis) peel, is currently recognized as a promising anti-inflammatory and anti-tumor agent. it is believed that, by undergoing metabolic biotransformation in vivo, nobiletin is demethylated by hepatic p450 enzymes, yielding multiple hydroxylated metabolites. however, it has not been possible to date to separate the two demethylated nobiletin metabolites, 3'-demethyl-nob and 4'-demethyl-nob (regio-isomers) on reversed-ph ... | 2006 | 16799930 |
| biocontrol of aflatoxins b1, b2, g1, g2, and fumonisin b1 with 6,7-dimethoxycoumarin, a phytoalexin from citrus sinensis. | phytoalexins (stress-induced compounds) from citrus sinensis cultivar valencia were screened for antifungal and antimycotoxic activity against a test organism (cladosporium cladosporoides) and mycotoxin-producing fungi fusarium verticillioides and aspergillus parasiticus. the active compound, a member of the coumarin family of compounds, has antifungal and antimycotoxic activities and was chemically identified. high-performance liquid chromatography results indicated that valencia oranges contai ... | 2006 | 16995528 |
| the role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit. | to investigate the relationship between a carotenoid profile and gene expression for carotenoid cleavage dioxygenases, three citrus varieties that exhibit different 9-cis-violaxanthin levels in their juice sacs, satsuma mandarin (citrus unshiu marc.; a variety accumulating a low level of 9-cis-violaxanthin), valencia orange (citrus sinensis osbeck; variety accumulating a high level of 9-cis-violaxanthin), and lisbon lemon (citrus limon burm.f.; a variety accumulating an undetectable level of 9-c ... | 2006 | 16714310 |
| recombinant expression and characterization of a xylella fastidiosa cysteine protease differentially expressed in a nonpathogenic strain. | xylella fastidiosa is a xylem-limited, gram-negative bacterium responsible for citrus variegated chlorosis (cvc) in sweet oranges. in the present study, we present the recombinant expression, purification and characterization of an x. fastidiosa cysteine protease (dubbed xylellain). the recombinant xylellain ((his)xylellain) was able to hydrolyze carbobenzoxy-phe-arg-7-amido-4-methylcoumarin (z-fr-mca) and carbobenzoxy-arg-arg-7-amido-4-methylcoumarin (z-rr-mca) with similar catalytic efficienci ... | 2006 | 16907719 |
| inhibition of aflatoxin b production of aspergillus flavus, isolated from soybean seeds by certain natural plant products. | the inhibitory effect of cowdung fumes, captan, leaf powder of withania somnifera, hyptis suaveolens, eucalyptus citriodora, peel powder of citrus sinensis, citrus medica and punica granatum, neem cake and pongamia cake and spore suspension of trichoderma harzianum and aspergillus niger on aflatoxin b(1) production by toxigenic strain of aspergillus flavus isolated from soybean seeds was investigated. | 2006 | 17032218 |
| polymethoxylated flavones induce ca(2+)-mediated apoptosis in breast cancer cells. | flavonoids, polyphenolic phytochemicals which include flavones and isoflavones, are present in the common human diet. it has been suggested that these compounds may exert anticancer activity; however, the mechanisms involved remain unknown. we have recently shown (sergeev, 2004, biochem biophys res commun 321: 462-467) that isoflavones can activate the novel apoptotic pathway mediated by cellular ca(2+). here, we report that polymethoxyflavones (pmfs) derived from sweet orange (citrus sinensis l ... | 2006 | 17046027 |
| quantitative real-time pcr for detection and identification of candidatus liberibacter species associated with citrus huanglongbing. | citrus huanglongbing (hlb, ex greening) is one of the most serious diseases of citrus. different forms of the disease are caused by different candidatus liberobacter species, candidatus liberibacter asiaticus (las), ca. l. africanus (laf) and ca. l. americanus (lam). the pathogen is transmitted by psyllid insects and by budding with contaminated plant materials. the vector psyllid diaphorina citri can transmit both las and lam. establishment of this vector into florida, reports of lam and las in ... | 2006 | 16414133 |
| calibration of sap flow estimated by the compensation heat pulse method in olive, plum and orange trees: relationships with xylem anatomy. | the compensation heat pulse method is widely used to estimate sap flow in conducting organs of woody plants. being an invasive technique, calibration is crucial to derive correction factors for accurately estimating the sap flow value from the measured heat pulse velocity. we compared the results of excision and perfusion calibration experiments made with mature olive (olea europaea l. 'manzanilla de sevilla'), plum (prunus domestica l. 'songal') and orange (citrus sinensis (l.) osbeck. 'cadener ... | 2006 | 16510387 |
| the complete chloroplast genome sequence of citrus sinensis (l.) osbeck var 'ridge pineapple': organization and phylogenetic relationships to other angiosperms. | the production of citrus, the largest fruit crop of international economic value, has recently been imperiled due to the introduction of the bacterial disease citrus canker. no significant improvements have been made to combat this disease by plant breeding and nuclear transgenic approaches. chloroplast genetic engineering has a number of advantages over nuclear transformation; it not only increases transgene expression but also facilitates transgene containment, which is one of the major impedi ... | 2006 | 17010212 |
| gene isolation, analysis of expression, and in vitro synthesis of glutathione s-transferase from orange fruit [citrus sinensis l. (osbeck)]. | glutathione s-transferases (gsts) (ec 2.5.1.18) are ubiquitous enzymes that have a defined role in xenobiotic detoxification, but a deeper knowledge of their function in endogenous metabolism is still lacking. in this work, we isolated the cdnas as well as the genomic clones of orange gsts. having considered gene organization and homology data, we suggest that the isolated gst gene is probably involved in the vacuolar import of anthocyanins. we also found that the blood and blond orange gsts sha ... | 2006 | 17117814 |
| [the relationship between activity and gene expression of phenylalanine ammonia-lyase and peel pitting in 'fengjie' navel orange fruits]. | citrus fruit is prone to develop peel pitting during development and storage, which greatly decreases its fresh market value because of the deterioration of the peel. in the present study, we have examined the effect of different temperatures (15 degrees c and 4 degrees c), waxing and mechanical damage on the changes in the activity of phenylalanine ammonia-lyase (pal) and the incidence of peel pitting in 'fengjie' navel orange (citrus sinensis osbeck) fruits. the expression levels of pal2, pal6 ... | 2006 | 16775409 |
| hydroxylated polymethoxyflavones and methylated flavonoids in sweet orange (citrus sinensis) peel. | polymethoxyflavones (pmfs) from citrus genus have been of particular interest because of their broad spectrum of biological activities, including antiinflammatory, anticarcinogenic, and antiatherogenic properties. there have been increasing interests in the exploration of health beneficial properties of pmfs in citrus fruits. therefore, the isolation and characterization of pmfs from sweet orange (citrus sinensis) peel will lead to new applications of the byproducts from orange juice processes a ... | 2006 | 16756344 |
| allergic reaction after ingestion of orange blossom pollen. | a 31-year-old atopic subject with allergic rhinoconjunctivitis with sensitization to several pollens, presented with urticaria and angioedema after ingestion of orange blossom pollen (citrus sinensis). a positive skin prick test for orange blossom pollen extract (bial-aristegui, bilbao, spain) at a concentration of 1.2 mg/ml was obtained. serum specific ige antibodies to orange blossom pollen were shown (unicap pharmacia system, uppsala, sweeden). a conjunctival provocation test was negative wit ... | 2006 | 16689190 |
| analysis of microsatellites in citrus unigenes. | simple sequence repeats (ssrs) were investigated in the unigene sequences from expressed sequence tags (est) of sweet orange (citrus sinensis osbeck), trifoliate orange (poncirus trifoliata raf.) and other citrus species and cultivars. a total of 37 802 citrus unigene sequences corresponding to 23.29 mb were searched, resulting in the identification of 8,218 ssrs. among them there were 4,913 (59.8%) mono-, 1,419 (17.3%) di-, 1,709 (20.8%) tri-, 114 (1.39%) tetra-, 23 (0.28%) penta- and 40 (0.49% ... | 2006 | 16625833 |
| gene characterization, analysis of expression and in vitro synthesis of dihydroflavonol 4-reductase from [citrus sinensis (l.) osbeck]. | dihydroflavonol 4-reductase (dfr, ec 1.1.1.219) catalyzes the reduction of dihydroflavonols to leucoanthocyanins, a key "late" step in the biosynthesis of anthocyanins. in this study we showed that a strong reduction in dfr expression occurs in the non-red orange cultivar (navel and ovale) compared to that of the red orange (tarocco) suggesting that the enzyme could be involved in the lack of production of anthocyanins. therefore, we isolated and compared the cdnas, the genomic clones, as well a ... | 2006 | 16524606 |
| germin-like protein cit s 1 and profilin cit s 2 are major allergens in orange (citrus sinensis) fruits. | oranges are clinically relevant allergenic foods. to date, orange allergens have not been characterized in detail. the study is aimed at analyzing the sensitization profile in orange-sensitized subjects with and without clinical allergy, and to identify orange allergens. fifty-six sensitized subjects with self-reported reactions to orange were grouped into reactors (anaphylaxis or multiple episodes of immediate reactions and/or positive challenge tests) and non-reactors (negative open food chall ... | 2006 | 16521162 |
| stomatal oscillations in orange trees under natural climatic conditions. | stomatal oscillations have been reported in many plant species, but they are usually induced by sudden step changes in the environment when plants are grown under constant conditions. this study shows that in navel orange trees (citrus sinensis) pronounced stomatal oscillations occur and persist under natural climatic conditions. | 2006 | 16478765 |
| fruit coloration difference between fengwan, a late-maturing mutant and its original cultivar fengjie72-1 of navel orange (citrus sinensis osbeck). | fruit color is one of the most important external quality traits. mutants with different color are useful for the study of regulating mechanism of coloration progress. a novel mutant, fengwan, derived from fengjie72-1 navel orange (citrus sinensis l. osbeck), has the distinctive characteristic of the coloration delay of 30 d or so, with the change in total soluble solid (tss) and the ratio of tss/acidity. in order to understand the mechanism underlying the difference of coloration between the mu ... | 2006 | 16477128 |
| mining and characterizing microsatellites from citrus ests. | freely available computer programs were arranged in a pipeline to extract microsatellites from public citrus est sequences, retrieved from the ncbi. in total, 3,278 bi- to hexa-type ssr-containing sequences were identified from 56,199 citrus ests. on an average, one ssr was found per 5.2 kb of est sequence, with the tri-nucleotide motifs as the most abundant. primer sequences flanking ssr motifs were successfully identified from 2,295 citrus ests. among those, a subset (100 pairs) were synthesiz ... | 2006 | 16474971 |
| vacuolar citrate/h+ symporter of citrus juice cells. | we have isolated a cdna, designated citrus sinensis citrate transporter 1 cscit1 encoding a novel vacuolar citrate/symporter. immunoblots using antibodies raised against cscit1 showed that the protein is localized to the juice sac cell vacuoles. the highest expression of cscit1 and the amount of protein in the juice sac cell vacuoles coincided with the developmental stage at which the vacuolar citrate content began declining with the concomitant increase in vacuolar ph. vacuoles from sacharomyce ... | 2006 | 16440212 |
| liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance as complementary analytical techniques for unambiguous identification of polymethoxylated flavones in residues from molecular distillation of orange peel oils (citrus sinensis). | liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance techniques with ultraviolet/diode array detection were used as complementary analytical tools for the reliable identification of polymethoxylated flavones in residues from molecular distillation of cold-pressed peel oils of citrus sinensis. after development of a liquid chromatographic separation procedure, the presence of several polymethoxy flavones such as sinensetin, nobiletin, tangeretin, quercetoge ... | 2006 | 16417279 |
| cloning and characterization of two 9-cis-epoxycarotenoid dioxygenase genes, differentially regulated during fruit maturation and under stress conditions, from orange (citrus sinensis l. osbeck). | there is now biochemical and genetic evidence that oxidative cleavage of cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (nced) is the critical step in the regulation of abscisic acid (aba) synthesis in higher plants. the peel of citrus fruit accumulates large amounts of aba during maturation. to understand the regulation of aba biosynthesis in citrus, two full-length cdnas (csnced1 and csnced2) encoding nceds were isolated and characterized from the epicarp of orange fruits (citrus si ... | 2006 | 16396998 |
| nobiletin: efficient and large quantity isolation from orange peel extract. | it is known that nobiletin possesses anticancer, antiviral and anti-inflammatory activities. recently, the demand for nobiletin in large quantities and high purity has increased. however, conventional normal-phase silica gel chromatography and c(18)-reverse-phase separation methods cannot satisfy the requirement of pure and gram scale nobiletin in a timely manner. in exploring the composition and the biological activities of polymethoxyflavones from sweet orange (citrus sinensis) peel, we develo ... | 2006 | 15999338 |
| evidence for host plant preference by iphiseiodes quadripilis (acari: phytoseiidae) on citrus. | in this study, we present field and laboratory evidence on the preference of iphiseiodes quadripilis (banks) for grapefruit (citrus paradisi macfadyen) leaves compared with sweet orange (citrus sinensis (l.) osbeck) leaves. this preference was confirmed in four orchards whether leaf samples were taken from either border trees of contiguous grapefruit or sweet orange or interior row trees with both citrus species in adjacent rows. iphiseiodes quadripilis was most abundant in grapefruit trees in s ... | 2006 | 16897564 |
| relationship between ploidy variation of citrus calli and competence for somatic embryogenesis. | this study focuses on the relationship between the genetic variation of calli and the competence for somatic embryogenesis in citrus. the dna content of 35 citrus calli of different genotypes was measured three times by flow cytometry during a period of four years. the results showed that 71.4 % of the genotypes had a progressive increase of varied cells, while those of page tangelo, shamouti sweet orange, russ navel orange and cleopatra decreased; significant difference in the variation degree ... | 2006 | 16875323 |
| pulsed electric fields versus thermal treatment: equivalent processes to obtain equally acceptable citrus juices. | pulsed electric field treatment has been claimed to produce more acceptable chilled citrus juices than those obtained by conventional thermal treatment. the pectin methylesterase activity and the acceptability of nine juices obtained from clementine mandarins, valencia oranges, and ortanique fruits (hybrid of mandarin and orange), untreated, pasteurized (85 degrees c for 10 s), and treated by pulsed electric fields (25 kv/cm for 330 micros), were evaluated. the treatments, selected to reach a si ... | 2006 | 16924935 |
| gus gene remains stable in transgenic citrus callus recovered from cryopreservation. | the conservation of transgenic materials is very important, paticularly for their potential future use in crop development. in this study, transgenic callus cultures of 'newhall' navel orange (citrus sinensis osbeck) were cryopreserved by a vitrification method. transgenic calluses survived cryopreservation and recovered under normal culture conditions. the results of pcr (polymerase chain reaction) amplification, southern blotting and sscp (single-strand conformation polymorphism) assay showed ... | 2006 | 14671689 |
| analysis of antioxidant compounds in sweet orange peel by hplc-diode array detection-electrospray ionization mass spectrometry. | hplc-diode array detection-electrospray ionization mass spectrometry was used to determine qualitatively and quantitatively the flavonoid content of several fractions and residues of extracts of greek navel sweet orange peel (citrus sinensis) from the region of southern greece (leonidi-tripoli). the main groups of flavonoids found according to hplc retention times, spectral data and literature references were polymethoxylated flavones, c-glycosylated flavones, o-glycosylated flavones, o-glycosyl ... | 2005 | 15515108 |