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molecular cloning, sequence characterization of a novel pepper gene nadp-icdh and its effect on cytoplasmic male sterility.nadp-dependent isocitrate dehydrogenase (nadp-icdh) is an important enzyme involved in energy metabolism. the complete coding sequence of the pepper (capsicum annuum) nadp-icdh gene was amplified using a reverse transcriptase pcr based on the conserved sequence information of the tomato and other solanaceae plants and known highly homologous pepper ests. nucleotide sequence analysis revealed that the pepper nadp-icdh gene encodes a protein of 415 amino acids that has high homology with the prote ...201222653649
antimicrobial potential of the ethiopian thymus schimperi essential oil in comparison with others against certain fungal and bacterial species.to evaluate the in vitro activities of ethiopian thymus schimperi with other three hydro distilled essential oils against dermatophytes (tricophyton spp. and microsporum spp.) and other pathogenic micro organisms.201526227483
preliminary examination of herbal extracts on the inhibition of helicobacter pylori.this work aims at describing the traditional uses, to determine the antimicrobial potential of two different extracts hexane, acetone of the leaves of citrus unshiu, citrus sinensis, citrus limon, laurus nobilis, citrus paradisi on clinical strain of h. pylori in a bid to identify potential sources of cheap starting materials for the synthesis of new drugs. h. pylori strain was a culture collection of hacettepe university, turkey.201424653559
a basic helix-loop-helix transcription factor, ptrbhlh, of poncirus trifoliata confers cold tolerance and modulates peroxidase-mediated scavenging of hydrogen peroxide.the basic helix-loop-helix (bhlh) transcription factors are involved in a variety of physiological processes. however, plant bhlhs functioning in cold tolerance and the underlying mechanisms remain poorly understood. here, we report the identification and functional characterization of ptrbhlh isolated from trifoliate orange (poncirus trifoliata). the transcript levels of ptrbhlh were up-regulated under various abiotic stresses, particularly cold. ptrbhlh was localized in the nucleus with transa ...201323624854
resistance to citrus canker induced by a variant of xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes.xanthomonas citri ssp. citri (x. citri) is the causal agent of asiatic citrus canker, a disease that seriously affects most commercially important citrus species worldwide. we have identified previously a natural variant, x. citri a(t) , that triggers a host-specific defence response in citrus limon. however, the mechanisms involved in this canker disease resistance are unknown. in this work, the defence response induced by x. citri a(t) was assessed by transcriptomic, physiological and ultrastr ...201627647752
the influence of glomus mosseae on tylenchulus semipenetrans-infected and uninfected citrus limon seedlings.greenhouse studies have shown that when rough lemon (citrus limon) seedlings infected with tyienchulus semipenetrans were transplanted into soil infested with glomus mosseae, the mycorrhizal fungus infection increased seedling growth compared to nonntycorrhizal seedlings. tylenchulus semipenetrans significantly suppressed seedling growth below that of mycorrhizal seedlings. histological observations of nematode-free mycorrhizal roots showed that hyphae penetrated the epidermis and invaded the co ...197919300642
characterization of a variant of xanthomonas citri subsp. citri that triggers a host-specific defense response.citrus is an economically important fruit crop that is severely afflicted by asiatic citrus bacterial canker (cbc), a disease caused by the phytopathogen xanthomonas citri subsp. citri (x. citri). to gain insight into the molecular epidemiology of cbc, 42 xanthomonas isolates were collected from a range of citrus spp. across 17 different orchards in tucumán, argentina and subjected to molecular, biochemical, and pathogenicity tests. analysis of genome-specific x. citri markers and dna polymorphi ...201323268580
evaluation of bioefficacy of three citrus essential oils against the dengue vector aedes albopictus (diptera: culicidae) in correlation to their components enantiomeric distribution.laboratory experiments were conducted to study the bioefficacy against ae. albopictus of three citrus essential oils, derived from peels of citrus sinensis, citrus limon, and citrus paradise and of their components. chiral gas chromatography analysis revealed the dominant occurrence of r-(+)-limonene and (-)-β-pinene in all three essential oils while in the case of lemon oil γ-terpinene, neral, and geranial detected also among other components. the tested citrus essential oils were toxic against ...201222903418
confined direct analysis in real time ion source and its applications in analysis of volatile organic compounds of citrus limon (lemon) and allium cepa (onion).the dart (direct analysis in real time) ion source is a novel atmospheric pressure ionization technique that enables efficient ionization of gases, liquids and solids with high throughput. a major limit to its wider application in the analysis of gases is its poor detection sensitivity caused by open-air sampling. in this study, a confined interface between the dart ion source outlet and mass spectrometer sampling orifice was developed, where the plasma generated by the atmospheric pressure glow ...201222499194
traditional phytoremedies for the treatment of menstrual disorders in district udhampur, j&k, india.herbal remedies form an integral part of healing and are considered to be the oldest forms of health care known to mankind. the present study aims to document traditional phytoremedies for the treatment of menstrual disorders in udhampur district of j&k, india.201525479156
a survey of plants responsible for causing irritant contact dermatitis in the amathole district, eastern cape, south africa.potentially harmful plants grow almost everywhere, hence, it is not practical to eradicate them all. however, a basic understanding of adverse cutaneous reactions and the common plants that cause each type can enable vulnerable individuals to discover the source of their dermatitis and thus prevent re-exposure. the aim of this study therefore, was to document the plants responsible for irritant contact dermatitis (icd) in the eastern cape, along with their respective irritants and clinical prese ...201425311272
behavioral effects of citrus limon and punica granatum combinations in rats.dietary supplements are becoming more influential as viable treatment for common chronic diseases and to promote normal development and functions of all system including brain. disorders like anxiety and depression may be managed through healthier variations is dietary pattern, since there are indications that diet rich in antioxidants and vitamins diminish anxiety and depression. hence this investigation was planned to assess the behavioral effects of citrus limon and punica granatum in two com ...201727510713
diosmetin triggers cell apoptosis by activation of the p53/bcl-2 pathway and inactivation of the notch3/nf-κb pathway in hepg2 cells.diosmetin (dios), a flavonoid compound, is abundant in citrus limon. emerging studies have shown that dios is an effective compound implicated in multiple types of cancer. however, whether dios serves a role in hepatocellular carcinoma (hcc) is still obscure. hepg2 cells were used in the present study, and it was observed that dios exhibited antitumor activity against liver cancer cells. western blotting was performed to evaluate cell apoptosis and survival-associated proteins, and the results d ...201628101238
host status of citrus and citrus relatives to tylenchulus graminis.studies were conducted on the host suitability of four citrus rootstocks--rough lemon (citrus limon), sour orange (c. aurantium), trifoliate orange (poncirus trifoliata cv. argentina), and swingle citrumelo (c. paradisi x p. trifoliata)--to tylenchulus graminis which was previously considered a "grass" race of t. semipenetrans. in an uncultivated field, sour orange seedlings grown with t. graminis-infected broomsedge (andropogon virginicus) were not infected with this nematode after 18-month's e ...198919287668
a transcriptionally active copia-like retroelement in citrus limon.the plant nuclear genome is largely composed of mobile dna, which can rearrange genomes and other individual gene structure and also affect gene regulation through various promoted activities: transposition, insertion, excision, chromosome breakage, and ectopic recombination. ty1-copia-like retrotransposon is a widespread class of transposable elements in the plant kingdom, representing a large part of the total dna content. here, a novel retrotransposon-like sequence was isolated and identified ...200919115051
improvement in growth and salt resistance of lemon (citrus limon) trees by an interstock-induced mechanism.interstocks can reduce toxic ion accumulations in leaves of budded citrus trees, but the mechanism is not understood. we grew sour orange (citrus aurantium l.; so) seedlings, budded trees of 'salustiano' orange (citrus sinensis l. osbeck; sao) on so, 'verna' lemon (citrus limon l. burm. f; vl/so) and interstock trees (vl/sao/so) in pots of sand watered with nutrient solution containing 5 (control) or 50 mm nacl (saline treatment) for 12 weeks. plants were harvested in six successive harvests and ...200314532011
effect of citrus lemon oil on growth and adherence of streptococcus mutans.in order to exploit novel anticaries agents, we investigated the effects of citrus lemon oil (clo), a type of natural product, on growth and adherence of the primary oral cariogenic bacteria streptococcus mutans (s. mutans). the growth inhibitory effect was explored with a micro-dilution assay. adherence was analyzed by colony counts on the respective surfaces and the adherence inhibition rate (air). real time-pcr was used to investigate the effects of clo on transcription of glucosyltransferase ...201323381618
dietary dehydrated lemon peel improves the immune but not the antioxidant status of gilthead seabream (sparus aurata l.).lemon (citrus limon) is the third most important species of citrus in the world, while spain is the major producer in europe. numerous beneficial effects of lemon are known, which explains their use in traditional medicine. the paper describes the effect of dietary dehydrated lemon peel (a sub-product of the lemon industry) on the growth, immune and antioxidant status of gilthead seabream (sparus aurata l.) over a period of 30 days. fish fed diets enriched with dehydrated lemon peel (1.5% and 3% ...201728359944
plants with potential use on obesity and its complications.obesity is the most prevalent nutritional disease and a growing public health problem worldwide. this disease is a causal component of the metabolic syndrome related with abnormalities, including hyperglycemia, dyslipidemia, hypertension, inflammation, among others. there are anti-obesity drugs, affecting the fundamental processes of the weight regulation; however they have shown serious side effects, which outweigh their beneficial effects. most recent studies on the treatment of obesity and it ...201526869866
in vitro biological evaluation of eight different essential oils against trypanosoma cruzi, with emphasis on cinnamomum verum essential oil.essential oils (eos) are complex mixtures of secondary metabolites from various plants. it has been shown that several eos, or their constituents, have inhibitory activity against trypanosomatid protozoa. thus, we analyzed the biological activity of different eos on trypanosoma cruzi, as well as their cytotoxicity on vero cells.201425148924
citrus chlorophyllase dynamics at ethylene-induced fruit color-break: a study of chlorophyllase expression, posttranslational processing kinetics, and in situ intracellular localization.fruit color-break is the visual manifestation of the developmentally regulated transition of chloroplasts to chromoplasts during fruit ripening and often involves biosynthesis of copious amounts of carotenoids concomitant with massive breakdown of chlorophyll. regulation of chlorophyll breakdown at different physiological and developmental stages of the plant life cycle, particularly at fruit color-break, is still not well understood. here, we present the dynamics of native chlorophyllase (chlas ...200818633118
antidiarrheal activity of hexane extract of citrus limon peel in an experimental animal model.acute diarrhea is one of the major illnesses that cause death in children, despite clinical interventions and the use of oral rehydration therapy. thus, there is need to discover other effective, affordable and accessible treatments for this disease. therefore, this study was carried out to investigate the effects of hexane extract of citrus limon peel (heclp) on castor oil-induced diarrhea in rats.201728285621
protective effects of essential oil of citrus limon against aspirin-induced toxicity in iec-6 cells.aspirin, one of the widely used nonsteroidal anti-inflammatory drugs, is the most highly consumed pharmaceutical product in the world. however, it has several side effects in cells. this study was designed to investigate the antioxidative activity and cytoprotective effects of essential oil of citrus limon (eoc) extracted from leaves against aspirin-induced damages in the rat small intestine epithelial cells (iec-6). biochemical indicators were used to assess cytotoxicity and oxidative damages c ...201628177735
effects of 20 selected fruits on ethanol metabolism: potential health benefits and harmful impacts.the consumption of alcohol is often accompanied by other foods, such as fruits and vegetables. this study is aimed to investigate the effects of 20 selected fruits on ethanol metabolism to find out their potential health benefits and harmful impacts. the effects of the fruits on ethanol metabolism were characterized by the concentrations of ethanol and acetaldehyde in blood, as well as activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in liver of mice. furthermore, potential hea ...201627043608
by-product identification and phytotoxicity of biodegraded direct yellow 4 dye.citrus limon peroxidase mediated decolourization of direct yellow 4 (dy4) was investigated. the process variables (ph, temperature, incubation time, enzyme dose, h2o2 amount, dye concentration, co-metal ions and surfactants) were optimized for maximum degradation of dye. maximum dye decolourization of 89.47% was achieved at ph 5.0, temperature 50 °c, enzyme dose 24 u/ml, h2o2 concentration 0.25 mm and dy4 concentration 18.75 mg/l and incubation time 10 min. the co-metal ions and surfactants did ...201727889513
ecophysiology of cuticular transpiration: comparative investigation of cuticular water permeability of plant species from different habitats.water permeabilities of astomatous, isolated cuticular membranes (cm) of 24 different plants species were measured. permeances varied from 1.7×10(-11) m·s(-1) (vanilla planifolia leaf) up to 2.1×10(-9) m·s(-1) (malus cf. domestica fruit) among different plant species, thus covering a range of over 2 orders of magnitude. ranking of species according to permeances resulted in four distinct groups. the first group, of species with the lowest cuticular transpiration rates, included evergreen species ...199628307383
metabolomic analysis of primary metabolites in citrus leaf during defense responses.mechanical damage is one of the unavoidable environmental stresses to plant growth and development. plants induce a variety of reactions which defend against natural enemies and/or heal the wounded sites. jasmonic acid (ja) and salicylic acid (sa), defense-related plant hormones, are well known to be involved in induction of defense reactions and play important roles as signal molecules. however, defense related metabolites are so numerous and diverse that roles of individual compounds are still ...201727789172
the protective effect of citrus limon essential oil on hepatotoxicity and nephrotoxicity induced by aspirin in rats.citrus limon is a member of the large rutaceae family characterized by its therapeutic proprieties and has been widely used in traditional medicine to treat various diseases. this study investigates the protective effect of citrus limon essential oil against a high dose of aspirin-induced acute liver and kidney damage in female wistar albino rats. twenty-eight adult female wistar rats were divided into 4 groups of 7 each: (1) a control group; (2) a group of rats which was kept untreated for 56da ...201627571876
anti-proliferative or cytopathic potential of thapsia garganica, citrus sinensis, citrus limon and vinca rosea extracts against human embryonic kidney carcinoma cell line.developing anti-cancer drugs from natural products is receiving increasing interest worldwide due to limitations and side-effects of anti-cancer drugs. the purpose of this study was to explore the anti-proliferative or cytopathic potential of natural compounds derived from plant sources as alternatives of synthetic compounds on human embryonic kidney carcinoma (hek) cell line.201627618522
effect of melia azedarach (sapindales: meliaceae) fruit extracts on citrus leafminer phyllocnistis citrella (lepidoptera: gracillariidae).melia azedarach l. extracts were studied in comparison with selected biorational insecticides against the citrus leafminer phyllocnistis citrella stainton under field conditions. citrus limon (l.) burm. f. trees were exposed to: melia extracts of green and mature fruits, neem oil (30% a.i.), abamectin (1.8% a.i.) and control. two sprays of each treatment (except for melia mature fruit extract) were executed at 10-d intervals. the live number of the 1(st) and later (2(nd) & 3(rd)) larval instars ...201323667805
mode of action of plastic film in extending life of lemon and bell pepper fruits by alleviation of water stress.the mechanism by which seal-packaging individual fruit in high density polyethylene film delays deterioration was investigated with lemon (citrus limon [l.] burm. f. cv eureka) and bell pepper (capsicum annuum l. cv maor) fruits. seal-packaging effects were due to the water-saturated atmosphere in the sealed enclosure around the fruit. softening of fruit was highly correlated with declining water potential of fruit. sealing drastically inhibited softening as well as changes in cell wall pectins. ...198316663193
inhibitory effect of essential oils against trichosporon ovoides causing piedra hair infection.piedra, is an asymptomatic fungal infection of the hair shaft, resulting in the formation of nodules of different hardness on the infected hair. the infection also known as trichomycosis nodularis is a superficial fungal infection arising from the pathogen being restricted to the stratum corneum with little or no tissue reaction. the nodules are a concretion of hyphae and fruiting bodies of the fungus. two varieties of piedra may be seen, black piedra and white piedra. the fungus trichosporon ov ...201224031963
traceability of 'limone di siracusa pgi' by a multidisciplinary analytical and chemometric approach.food traceability is increasingly relevant with respect to safety, quality and typicality issues. lemon fruits grown in a typical lemon-growing area of southern italy (siracusa), have been awarded the pgi (protected geographical indication) recognition as 'limone di siracusa'. due to its peculiarity, consumers have an increasing interest about this product. the detection of potential fraud could be improved by using the tools linking the composition of this production to its typical features. th ...201627283690
efficacy of a nasal spray from citrus limon and cydonia oblonga for the treatment of hay fever symptoms-a randomized, placebo controlled cross-over study.nasal spray from lemon and quince (lqns) is used to treat hay fever symptoms and has been shown to inhibit histamine release from mast cells in vitro. forty-three patients with grass pollen allergy (gpa) were randomized to be treated either with placebo or lqns for one week, respectively, in a cross-over study. at baseline and after the respective treatments patients were provoked with grass pollen allergen. outcome parameters were nasal flow measured with rhinomanometry (primary), a nasal sympt ...201627282490
citrus limon-derived nanovesicles inhibit cancer cell proliferation and suppress cml xenograft growth by inducing trail-mediated cell death.nanosized vesicles are considered key players in cell to cell communication, thus influencing physiological and pathological processes, including cancer. nanovesicles have also been found in edible-plants and have shown therapeutic activity in inflammatory bowel diseases; however information on their role in affecting cancer progression is missing.our study identify for the first time a fraction of vesicles from lemon juice (citrus limon l.), obtained as a result of different ultracentrifugation ...201526098775
effect of sample pretreatment on the extraction of lemon (citrus limon) components.a study on the key role of lemon sample pretreatment on the analytical results is here presented. the objective of the study was to analyze the differences between extracts obtained from lyophilized and air-dried samples-the most common sample pretreatment in citrus studies-in comparison to extracts from fresh samples. all the extracts were obtained with ultrasound assistance and analyzed by lc-qtof ms/ms. the dataset, constituted by 74 tentative identified metabolites, was first evaluated by an ...201627130132
chemical profile and antioxidant properties of extracts and essential oils from citrus × limon (l.) burm. cv. femminello comune.citrus × limon cv. femminello comune (rutaceae) from rocca imperiale (italy), one of the six protected geographical indication (pgi) italian lemon crops, has been recently received renewed interest. in this work, fresh and dried peels and leaves were extracted by hydrodistillation, supercritical fluid extraction (sfe), and soxhlet apparatus. chemical profile was assessed by gas chromatography (gc) and gas chromatography/mass spectrometry (gc/ms). except for leaves extracts obtained by soxhlet ap ...201627112122
the use of aromasticks at a cancer centre: a retrospective audit.to consider the use of aromasticks in a cancer centre in the uk: the reasons for their use, the choice of essential oils used in them and the demographics of the patients to whom they were given.201425486854
parasitoids and hyperparasitoids (hymenoptera) on aphids (hemiptera) infesting citrus in east mediterranean region of turkey.the aphids, aphid parasitoids, and hyperparasitoids found in citrus orchards, the parasitoids' and hyperparasitoids' seasonal abundance, and the plant-aphid-parasitoid relationships in hatay, osmaniye, adana, and mersin provinces of the east mediterranean region of turkey are presented in the present 2-yr study. aphidius colemani viereck, binodoxys angelicae (haliday), and lysiphlebus confusus tremblay and eady (hymenoptera: braconidae: aphidiinae) were encountered as the most common parasitoids ...201425480969
in vitro and in vivo effect of citrus limon essential oil against sarcoptic mange in rabbits.the effect of lemon oil (citrus limon) on sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. the mite samples were collected from naturally infected rabbits. the lemon oil was prepared in six concentrations by dilution with distilled water (2.5, 5, 10, 20, 50, and 100 %). in vitro application was done in five replicates for each concentration in petri dishes in the laboratory. the treated mites were observed at 1, 12, and 24 h post application (pa) for lemon oil effect. in addit ...201627098160
chemical characterization of citrus limon var. pompia and incorporation in phospholipid vesicles for skin delivery.the components of pompia, a hybrid citrus species cultivated only in sardinia (italy), were extracted using an environmentally-friendly method and food-grade solvents. taking into account that only few data are available on pompia composition, the phytochemical fingerprint of its rind extract was obtained by accurate component separation and identification, combining hplc and mass spectrometry. different flavones such as naringin (23.77μg/mg), neoeriocitrin (46.53μg/mg) and neohesperidin (44.57μ ...201627084291
comparative study of the effect of sample pretreatment and extraction on the determination of flavonoids from lemon (citrus limon).flavonoids have shown to exert multiple beneficial effects on human health, being also appreciated by both food and pharmaceutical industries. citrus fruits are a key source of flavonoids, thus promoting studies to obtain them. characteristics of these studies are the discrepancies among sample pretreatments and among extraction methods, and also the scant number of comparative studies developed so far.201626807979
phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates.the search for new antimicrobial compounds is ongoing. its importance cannot be overemphasized in an era of emerging resistant pathogenic organisms. this study therefore investigated the phytochemical composition and antioxidant and antimicrobial activities of different citrus juice concentrates. fruit juices of citrus tangerine (tangerine), citrus paradisi (grape), citrus limon (lemon), and citrus aurantifolia (lime) were evaluated. antimicrobial activities against five bacterial and three fung ...201626788316
comparison of heat and mass transfer of different microwave-assisted extraction methods of essential oil from citrus limon (lisbon variety) peel.dried and fresh peels of citrus limon were subjected to microwave-assisted hydrodistillation (mahd) and solvent-free microwave extraction (sfme), respectively. a comparison was made between mahd and sfme with the conventional hydrodistillation (hd) method in terms of extraction kinetic, chemical composition, and antioxidant activity. higher yield results from higher extraction rates by microwaves and could be due to a synergy of two transfer phenomena: mass and heat acting in the same way. gas c ...201526788292
citrus limon extract: possible inhibitory mechanisms affecting testicular functions and fertility in male mice.the effect of oral administration of 50% ethanolic leaf extract of citrus limon (500 and 1,000 mg/kg body weight/day) for 35 days on fertility and various male reproductive endpoints was evaluated in parkes strain of mice. testicular indices such as histology, 3β- and 17β-hsd enzymes activity, immunoblot expression of star and p450scc, and germ cell apoptosis by tunel and casp- 3 expression were assessed. motility, viability, and number of spermatozoa in the cauda epididymidis, level of serum te ...201626787324
characterization of lemon (citrus limon) polar extract by liquid chromatography-tandem mass spectrometry in high resolution mode.eighty four metabolites (32 flavonoids, 15 amino acids, nine carboxylic acids, six coumarins, six sugars, five phenolic acids and 11 unclassified compounds) have been tentatively identified in a polar extract from lemon, without reference standards, based on their liquid chromatography-quadrupole-time-of-flight ms/ms spectra and the comparison with databases. despite information in databases for some families of plant compounds is poor, tentative identification based on ms/ms information (mass o ...201526505764
differential tolerance of 3 self-rooted citrus limon cultivars to nacl stress.one-year-old self-rooted cuttings of three citrus limon cultivars (nouvel athos, lisbon, maglini) were grown in 1 l black plastic bags, containing a mixture of sand: perlite (1:1), in order to investigate: i) if genotypic differences to salt stress existed, ii) if kno3 can alleviate salinity stress, iii) the role of carbohydrates (such as the sugars fructose, glucose and sucrose) and proline as possible osmoregulators in c. limon osmoprotection, and iv) if genotypic differences to salt stress to ...201526476793
beverages of lemon juice and exotic noni and papaya with potential for anticholinergic effects.lemon (citrus limon (l.) burm. f.) juice beverages enriched either with noni (morinda citrifolia l.) (ln) or papaya (carica papaya l.) (lp), were characterized by hplc-dad-esi/ms(n), the antioxidant capacity was evaluated by (dpph·), superoxide (o2(·-)), hydroxyl radicals (·oh) and hypochlorous acid (hocl) assays, and their potential as acetylcholinesterase (ache) and butyrylcholinesterase (buche) inhibitors was also assessed. the fruits are rich in a wide range of bioactive phenolics. regarding ...201525306312
inhibition of microbial pathogens using fruit and vegetable peel extracts.the aim of the present work is to evaluate the antimicrobial potency of some vegetable and fruit peels. the extraction was done by individual cold percolation method using various solvents with increasing polarity (hexane, ethyl acetate, acetone, methanol and aqueous). the antimicrobial activity was done by agar well diffusion assay against five gram positive bacteria, five gram negative bacteria and four fungi. all extracts demonstrated varied level of antimicrobial activity. the peel extracts ...201424725235
development and reproduction of panonychus citri (prostigmata: tetranychidae) on different species and varieties of citrus plants.the species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite panonychus citri (mcgregor) (prostigmata: tetranychidae) and alter the pest management programs in citrus groves. in this study we evaluated, in the laboratory, the development and reproduction of p. citri and estimated its life table parameters when reared on four varieties of citrus sinensis (l.) osbeck (valencia, pera, natal, and hamlin), one variety of citrus reticulata ...201526459376
effect on blood pressure of daily lemon ingestion and walking.background. recent studies suggest that the daily intake of lemon (citrus limon) has a good effect on health, but this has not been confirmed in humans. in our previous studies, it was observed that people who are conscious of their health performed more lemon intake and exercise. an analysis that took this into account was required. methodology. for 101 middle-aged women in an island area in hiroshima, japan, a record of lemon ingestion efforts and the number of steps walked was carried out for ...201424818015
accumulation of putrescine during chilling injury of fruits.putrescine (put) increased 68% in lemon (citrus limon (l.) burm. f. cv bearss) flavedo, 39% in grapefruit (c. paradisi macf. cv marsh) flavedo, 49% in grapefruit juice, and 149% in pepper (capsicum annuum l. cv early calwonder) pericarp when fruits were stored at chilling temperatures. in lemon flavedo, the coefficient of correlation (r(2)) between put concentration with severity of chilling was 0.90 and put levels almost doubled; the injury index going from 1 to 2 units. pepper pericarp, which ...198616665015
does citrus leaf miner impair hydraulics and fitness of citrus host plants?gas exchange and hydraulic features were measured in leaves of three different citrus species (citrus aurantium l., citrus limon l., citrus  ×  paradisii macfad) infested by phyllocnistis citrella staiton, with the aim to quantify the impact of this pest on leaf hydraulics and, ultimately, on plant fitness. infested leaves were characterized by the presence on the leaf blade of typical snake-shaped mines and, in some cases, of a crumpled leaf blade. light microscopy showed that leaf crumpling wa ...201324319027
the mir396b of poncirus trifoliata functions in cold tolerance by regulating acc oxidase gene expression and modulating ethylene-polyamine homeostasis.micrornas (mirnas) are non-coding regulatory molecules that play important roles in a variety of biological processes. although a number of cold-responsive mirnas have been computationally identified, functions and mechanisms of most mirnas are not well understood. herein, the function of trifoliate orange [poncirus trifoliata (l.) raf.] mirna396b (ptr-mir396b) in cold tolerance and its potential regulatory module were investigated. compared with the wild type (wt), transgenic lemon (citrus limo ...201627402968
traditional alcoholic beverages and their value in the local culture of the alta valle del reno, a mountain borderland between tuscany and emilia-romagna (italy).traditional alcoholic beverages (tabs) have only received marginal attention from researchers and ethnobotanists so far, especially in italy. this work is focused on plant-based tabs in the alta valle del reno, a mountainous area on the border between tuscany and emilia-romagna regions. the aims of our study were to document local knowledge about tabs and to analyze and discuss the distribution of related knowledge within the investigated communities.201627333772
the in vitro effect of selected essential oils on the growth and mycotoxin production of aspergillus species.the aim of the present study was to assess the antifungal and anti-toxinogenic activity of 15 essential oils (eos) against three fungi of the genus aspergillus (a. parasiticus kmi-227-lr, a. parasiticus kmi-220-lr and a. flavus kmi-202-lr). the minimum inhibitory doses (mids) of the tested essential oils and their antifungal activity were determined using the micro-atmosphere method. the original commercial essential oil samples of jasminum officinale l., thymus vulgaris l., syzygium aromaticum ...201627322876
antifungal effect of essential oils against fusarium keratitis isolates.the present study was carried out to investigate the antifungal effects of cinnamomum zeylanicum, citrus limon, juniperus communis, eucalyptus citriodora, gaultheria procumbens, melaleuca alternifolia, origanum majorana, salvia sclarea, and thymus vulgaris essential oils against fusarium species, the most common etiologic agents of filamentous fungal keratitis in south india. c. zeylanicum essential oil showed strong anti-fusarium activity, whereas all the other tested essential oils proved to b ...201526227503
identification of hop stunt viroid infecting citrus limon in china using small rnas deep sequencing approach.the advent of next generation sequencing technology has allowed for significant advances in plant virus discovery, particularly for identification of covert viruses and previously undescribed viruses. the citrus limon burm. f. (c. limon) is a small evergreen tree native to asia, and . china is the world's top lemon-producing nation.201526148502
systematic hplc/esi-high resolution-qtof-ms methodology for metabolomic studies in nonfluorescent chlorophyll catabolites pathway.characterization of nonfluorescent chlorophyll catabolites (nccs) and dioxobilane-type nonfluorescent chlorophyll catabolite (dncc) in peel extracts of ripened lemon fruits (citrus limon l.) was performed by hplc/esi-high resolution-qtof-ms method. compounds were identified in samples on the basis of measured accurate mass, isotopic pattern, and characteristic fragmentation profile with an implemented software postprocessing routine. three ncc structures already identified in other vegetal tissu ...201525741450
identification, synthesis, and characterization of novel sulfur-containing volatile compounds from the in-depth analysis of lisbon lemon peels (citrus limon l. burm. f. cv. lisbon).lemons (citrus limon) are a desirable citrus fruit grown and used globally in a wide range of applications. the main constituents of this sour-tasting fruit have been well quantitated and characterized. however, additional research is still necessary to better understand the trace volatile compounds that may contribute to the overall aroma of the fruit. in this study, lisbon lemons (c. limon l. burm. f. cv. lisbon) were purchased from a grove in california, usa, and extracted by liquid-liquid ex ...201525639384
memory boosting effect of citrus limon, pomegranate and their combinations.memory is greatly influenced by factors like food, stress and quality of sleep, hence present study was designed to evaluate the effect of citrus limon and pomegranate juices on memory of mice using harvard panlab passive avoidance response apparatus controlled through le2708 programmer. passive avoidance is fear-motivated tests used to assess short or long-term memory of small animals, which measures latency to enter into the black compartment. animals at mcld showed highly significant and sign ...201425362607
phospholipases a2: enzymatic assay for snake venom (naja naja karachiensis) with their neutralization by medicinal plants of pakistan.phospholipases a2 (pla2) are the most lethal and noxious component of naja naja karachiensis venom. they are engaged to induce severe toxicities after their penetration in victims. present study was designed to highlight hydrolytic actions of pla. in an egg yolk mixture and to encounter their deleterious effects via medicinal plants of pakistan. pla2 were found to produce free fatty acids in a dose dependent manner. venom at concentration of 0.1 mg was found to liberate 26.6 pmoles of fatty acid ...201425272888
toxic effect of citrus peel constituents on anastrepha fraterculus wiedemann and ceratitis capitata wiedemann immature stages.the toxicity of essential oils from the citrus peel has been proposed as the major resistance mechanism offered by citrus to fruit fly infestation. we evaluated the insecticidal activity of the ether extracts from the lemon (citrus limon [l.] burm.) and grapefruit (c. paradisi macfadyen) peel as well as from limonene and citral against anastrepha fraterculus (wiedemann) and ceratitis capitata (wiedemann) immature stages. we also evaluated the toxicity of the extracts at two ripening stages. extr ...201425237738
voltammetric trace determination of mercury using plant refuse modified carbon paste electrodes.citrus limon peel (kitchen waste) and leucaena leucocephala seeds (agricultural waste) were used as a modifier for fabrication of modified carbon paste electrode for determination of mercury in aqueous sample using differential pulse anodic stripping voltammetry. mercury was adsorbed on electrode surface at open circuit and anodic stripping voltammetric scan was run from -0.5 to 0.5 v. various electrochemical parameters including amount of modifier, supporting electrolyte, accumulating solvent, ...201323709264
behavioral effects of citrus limon in rats.anxiety and depression are increasing worldwide, however these disorders may be managed by making healthier changes is dietary pattern, since there are evidences that diet rich in antioxidants and vitamins help reduce anxiety and depression. hence present study was designed to evaluate the behavioral effects of citrus limon in rats at three different doses i.e. 0.2, 0.4 and 0.6 ml/kg considered as low, moderate and high doses. anxiolytic and antidepressant activities were specifically assessed t ...201525227172
salicylic acid and methyl jasmonate improve chilling tolerance in cold-stored lemon fruit (citrus limon).chilling injury (ci) is associated with the degradation of membrane integrity which can be aligned to phenolic oxidation activated by polyphenol oxidase (ppo) and peroxidase (pod), enzymes responsible for tissue browning. phenylalanine ammonia-lyase (pal) is a further enzyme prominent in the phenolic metabolism that is involved in acclimation against chilling stress. it was hypothesized that treatment with methyl jasmonate (mj) and salicylic acid (sa) may enhance chilling tolerance in lemon frui ...201425216124
adoption of exotic plants by an endemic australian weevil, orthorhinus cylindrirostris (coleoptera: curculionidae).the elephant weevil orthorhinus cylindrirostris (f.) (coleoptera: curculionidae) is endemic to australia and has colonized several exotic crops including citrus, blueberries, and grapevines. we compared the life history of this pest on two native and two exotic host plants: hickory wattle acacia falcata willdenow, rough-barked apple angophora floribunda (smith) sweet, lemon citrus limon (l.) burman, and blueberry vaccinium corymbosum (l.). blueberry was attacked more often (22 of the 24 plants) ...201425198945
conversion of protopanaxadiol type saponins to ginsenoside rg3 by lemon.the ability of fresh lemon (citrus limon) to convert protopanaxadiol-type saponins into ginsenoside rg3 was investigated, and the structures of 20(s)-ginsenoside rg3 (1) and 20(r)-ginsenoside rg3 (2) were identified by 1h nmr and 13c nmr spectroscopy. the experiment showed that lemon possesses the strong ability to hydrolyze ginsenosides. when protopanaxadiol-type saponins (16 mg/ml) were hydrolyzed by fresh lemon juice at 80 degrees c for 3 hrs, the conversion ratios of ginsenoside rb1, rb2, rc ...201223074894
characterization of coumarin-specific prenyltransferase activities in citrus limon peel.coumarins, a large group of polyphenols, play important roles in the defense mechanisms of plants, and they also exhibit various biological activities beneficial to human health, often enhanced by prenylation. despite the high abundance of prenylated coumarins in citrus fruits, there has been no report on coumarin-specific prenyltransferase activity in citrus. in this study, we detected both o- and c-prenyltransferase activities of coumarin substrates in a microsome fraction prepared from lemon ...201222785469
molecular cloning and characterization of a geranyl diphosphate-specific aromatic prenyltransferase from lemon.prenyl residues confer divergent biological activities such as antipathogenic and antiherbivorous activities on phenolic compounds, including flavonoids, coumarins, and xanthones. to date, about 1,000 prenylated phenolics have been isolated, with these compounds containing various prenyl residues. however, all currently described plant prenyltransferases (pts) have been shown specific for dimethylallyl diphosphate as the prenyl donor, while most of the complementary dnas encoding these genes hav ...201425077796
utility of indels for species-level identification of a biologically complex plant group: a study with intergenic spacer in citrus.the consortium of barcode of life plant working group proposed to use the defined portion of plastid genes rbcl and matk either singly or in combination as the standard dna barcode for plants. but dna barcode based identification of biologically complex plant groups are always a challenging task due to the occurrence of natural hybridization. here, we examined the use of indels polymorphism in trnh-psba and trnl-trnf sequences for rapid species identification of citrus. dna from young leaves of ...201425048292
variability of 137cs and 40k soil-to-fruit transfer factor in tropical lemon trees during the fruit development period.in this investigation we evaluate the soil uptake of (137)cs and (40)k by tropical plants and their consequent translocation to fruits, by calculating the soil-to-fruit transfer factors defined as f(v) = [concentration of radionuclide in fruit (bq kg(-1) dry mass)/concentration of radionuclide in soil (bq kg(-1) dry mass in upper 20 cm)]. in order to obtain f(v) values, the accumulation of these radionuclides in fruits of lemon trees (citrus limon b.) during the fruit growth was measured. a math ...201222115437
in vitro/in vivo effect of citrus limon (l. burm. f.) juice on blood parameters, coagulation and anticoagulation factors in rabbits.the genus citrus of the family rutaceae includes many species e.g. citrus indica, citrus aurantifolia and citrus limon, among which citrus limon l. burm. f. has been reported to have highest antimicrobial activity. it is used as antidote against certain venom, due to its platelet inhibitory effect and also reported to have hypocholesterolemic effect. however its anticoagulant and thrombolytic effect were not been investigated, hence a prospective in-vitro/in-vivo study was designed to determine ...201425015459
chemical guide parameters for spanish lemon (citrus limon (l.) burm.) juices.to contribute for setting reference guideline for commercial juice extracted from the spanish lemon varieties, chemical composition of 92 direct and 92 reconstituted samples were investigated. in direct lemon juice, titratable acidity was 52.4 g/l, being the citric acid the main component. glucose, fructose and sucrose concentrations were 7.9, 7.3 and 4.5 g/l, respectively. predominant mineral was potassium (1264.2mg/l), followed by phosphorous (306 mg/l), calcium (112 mg/l) and magnesium (92.6 ...201424874375
expression of the h+-atpase aha10 proton pump is associated with citric acid accumulation in lemon juice sac cells.the sour taste of lemons (citrus limon (l.) burm.) is determined by the amount of citric acid in vacuoles of juice sac cells. faris is a "sweet" lemon variety since it accumulates low levels of citric acid. the university of california riverside citrus variety collection includes a faris tree that produces sweet (faris non-acid; fna) and sour fruit (faris acid; fa) on different branches; it is apparently a graft chimera with layer l1 derived from millsweet limetta and layer l2 from a standard le ...201121556928
does antioxidant properties of the main component of essential oil reflect its antioxidant properties? the comparison of antioxidant properties of essential oils and their main components.this study discusses the similarities and differences between the antioxidant activities of some essential oils: thyme (thymus vulgaris), basil (ocimum basilicum), peppermint (mentha piperita), clove (caryophyllus aromaticus), summer savory (satureja hortensis), sage (salvia hispanica) and lemon (citrus limon (l.) burm.) and of their main components (thymol or estragole or menthol or eugenol or carvacrol or camphor or limonene) estimated by using 2,2'-diphenyl-1-picrylhydrazyl, 2,2'-azinobis(3-e ...201424849850
insecticidal activity of plant essential oils against the vine mealybug, planococcus ficus.the vine mealybug, planococcus ficus (signoret) (hemiptera: pseudococcidae), is a pest in grape vine growing areas worldwide. the essential oils from the following aromatic plants were tested for their insecticidal activity against p. ficus: peppermint, mentha piperita l. (lamiales: lamiaceae), thyme-leaved savory, satureja thymbra l., lavender, lavandula angustifolia mill, and basil, ocimum basilicum l. essential oils from peels of the following fruits were also tested: lemon, citrus limon l. ( ...201324766523
biomimetic synthesis of silver nanoparticles by citrus limon (lemon) aqueous extract and theoretical prediction of particle size.in the present study, silver nanoparticles were rapidly synthesized at room temperature by treating silver ions with the citrus limon (lemon) extract. the effect of various process parameters like the reductant concentration, mixing ratio of the reactants and the concentration of silver nitrate were studied in detail. in the standardized process, 10(-2)m silver nitrate solution was interacted for 4h with lemon juice (2% citric acid concentration and 0.5% ascorbic acid concentration) in the ratio ...201120833002
beneficial effect of citrus limon peel aqueous methanol extract on experimentally induced urolithic rats.citrus limon (l.) burm.f. (rutaceace) is a commonly available fruit variety with high medicinal and industrial values.201626452728
pathogen infection drives patterns of nutrient resorption in citrus plants.nutrient resorption processes in the plants infected by pathogen remain poorly understood. huanglongbing (hlb) is a destructive disease of citrus. hlb-pathogen 'candidatus liberibacter asiaticus' grows specifically in the phloem of hosts and may cause problems in the plant vascular system after infection. therefore, it brings a great concern about the phloem nutrient transport and nutrient intra-cycling in hlb-affected plants. we investigated the effects of 'ca. l. asiaticus' infection on nitrog ...201526419510
oxidative stability and alpha-tocopherol retention in soybean oil with lemon seed extract (citrus limon) under thermoxidation.the synergistic effect of lemon seed extract with tert-butylhydroquinone (tbhq) in soybean oil subjected to thermoxidation by rancimat was investigated, and the influence of these antioxidants on a-tocopherol degradation in thermoxidized soybean oil. control, lse (2400 mg/kg lemon seed extract), tbhq (50 mg/kg), mixture 1 (lse + 50 mg/kg tbhq) and mixture 2 (lse + 25 mg/kg tbhq) were subjected to 180 degrees c for 20 h. samples were taken at time 0, 5, 10, 15 and 20 h intervals and analysed for ...200919967989
developmental stimuli and stress factors affect expression of clglp1, an emerging allergen-related gene in citrus limon.germins and germin-like proteins (glps) constitute an ubiquitous family of plant proteins that seem to be involved in many developmental and stress related processes. a novel glp cdna was isolated from citrus limon and structural features and genomic organization were investigated by in silico and southern blots analysis. in lemon, the clglp1 encodes a 24.38 kda which possesses a conserved motif of plant glps proteins. a phylogetic analysis mapped clglp1 as belonging to the ger3 subfamily into t ...201424681751
"singing in the tube"--audiovisual assay of plant oil repellent activity against mosquitoes (culex pipiens).plant essential oils have been suggested as a promising alternative to the established mosquito repellent deet (n,n-diethyl-meta-toluamide). searching for an assay with generally available equipment, we designed a new audiovisual assay of repellent activity against mosquitoes "singing in the tube," testing single mosquitoes in drosophila cultivation tubes. statistics with regression analysis should compensate for limitations of simple hardware. the assay was established with female culex pipiens ...201626412058
an ethnobotanical survey of medicinal plants in trinidad.an ethnobotanical survey was conducted on the caribbean island of trinidad to identify medicinal plants commonly used in traditional medicine to treat a variety of medical conditions.201526369926
experimental evidence and in silico identification of tryptophan decarboxylase in citrus genus.plant tryptophan decarboxylase (tdc) converts tryptophan into tryptamine, precursor of indolealkylamine alkaloids. the recent finding of tryptamine metabolites in citrus plants leads to hypothesize the existence of tdc activity in this genus. here, we report for the first time that, in citrus x limon seedlings, deuterium labeled tryptophan is decarboxylated into tryptamine, from which successively deuterated n,n,n-trimethyltryptamine is formed. these results give an evidence of the occurrence of ...201728208655
chemical composition of different botanical origin honeys produced by sicilian black honeybees (apis mellifera ssp. sicula).in 2008 a slow food presidium was launched in sicily (italy) for an early warning of the risk of extinction of the sicilian native breed of black honeybee (apis mellifera l. ssp sicula). today, the honey produced by these honeybees is the only sicilian honey produced entirely by the black honeybees. in view of few available data regarding the chemical composition of a. mellifera ssp. sicula honeys, in the present investigation the chemical compositions of sulla honey (hedysarum coronarium l.) an ...201525730368
potentiating effects of honey on antioxidant properties of lemon-flavoured black tea.health benefits including antioxidant potential of black tea (camellia sinensis), lemon (citrus limon) and honey bees (apis mellifera) have been extensively reported. nevertheless, nothing is reported about the effects of their concomitant use. herein, those effects were evaluated in infusions of lemon-flavoured black tea with three different kinds of honey (light amber, amber and dark amber) from lavandula stoechas, erica sp. pl. and other indigenous floral species from north-east portugal, a r ...201322856394
cheminformatics based selection and cytotoxic effects of herbal extracts.bioinformatics and traditional medicine can be used in discovery and design of novel candidate drugs to efficient cancer chemotherapy. in this study, similarity search tools employed to screen and introduce novel herbs with antitumor property. several novel herbs have been selected by using logical computational algorithms and assayed on six cancerous cell lines. complementary assays involved hemolytic and antifungal mic tests have been performed to determine selectivity and their biocompatibili ...200919602433
oral dose of citrus peel extracts promotes wound repair in diabetic rats.diabetic patients wound healing is slower than the healthy individuals. three citrus peel extracts; lemon (citrus limon), grapes fruits (citrus paradise) and orange (citrus sinensis) promote wound healing in experimental animals. this study investigated the effect of oral treatment with citrus peel extracts on wound repair of the skin of diabetic rats. the extracts were estimated for vitamin c and total carotenoid contents prior to animal study. diabetes mellitus was induced in rats by intraperi ...201324506007
characterization of basidiomycetes associated with wood rot of citrus in southern italy.the characterization of basidiomycetes associated with wood rots in commercial citrus orchards in southern italy revealed that both white and brown rot fungi are implicated in this disease. fomitiporia mediterranea was the most prevalent species causing a white rot, followed by fomitopsis sp. which, by contrast, was associated with brown rot wood decay. furthermore, phellinus spp. and other nonidentified basidiomycetous fungi showing genetic affinity with the genera phellinus and coniophora were ...201424502208
eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis.lemon (citrus limon) contains various bioactive flavonoids, and prevents obesity and obesity-associated metabolic diseases. we focused on eriocitrin (eriodictyol 7-rutinoside), a powerful antioxidative flavonoid in lemon with lipid-lowering effects in a rat model of high-fat diet. to investigate the mechanism of action of eriocitrin, we conducted feeding experiments on zebrafish with diet-induced obesity. oral administration of eriocitrin (32 mg/kg/day for 28 days) improved dyslipidaemia and dec ...201424424211
micropropagation of seedless lemon (citrus limon l. cv. kaghzi kalan) and assessment of genetic fidelity of micropropagated plants using rapd markers.a micropropagation protocol was developed for multiplication of seedless lemon (citrus limon l. cv. kaghzi kalan) using nodal explants. the maximum shoot regeneration was observed on low level of bap (0.1 mg l(-1)) or kinetin (0.5 mg l(-1)). ba was recorded to be better than kinetin in terms of number of days taken to bud break. the maximum number of shoots per explant was observed on 0.1 mg l(-1) ba and 0.5 mg l(-1) kinetin. shoot proliferation decreased with increasing concentration of ba alon ...201324381445
spatial and temporal variation in chaetanaphothrips orchidii moulton (thysanoptera: thripidae) population and its damage on lemon.chaetanaphothrips orchidii moulton has recently been detected in lemon (citrus limon) orchards in northwest argentina, causing high levels of damage on fruits. severe damage results in the rejection of fruit for export, which must then be sold in the industry. however, the restrictions imposed by the citrus industry on insecticide residues sometimes also result in fruit rejection. here, we studied the ecology and behavior of c. orchidii in order to propose a pest management strategy that could m ...201323949715
host susceptibility of citrus cultivars to queensland fruit fly (diptera: tephritidae).citrus crops are considered to be relatively poor hosts for queensland fruit fly, bactrocera tryoni (froggatt), as for other tephritid species. australian citrus growers and crop consultants have reported observable differences in susceptibility of different citrus cultivars under commercial growing conditions. in this study we conducted laboratory tests and field surveys to determine susceptibility to b. tryoni of six citrus cultivars [(eureka lemon (citrus limon (l.) osbeck); navel and valenci ...201323786078
[effects of different planting modes on the soil permeability of sloping farmlands in purple soil area].taking bare land as the control, this paper studied the effects of different planting modes on the soil permeability of sloping farmlands in purple soil area. for the test six planting modes, the soil permeability was in the order of eriobotrya japonica > citrus limon > vetiveria zizanioides hedgerows +corn >leucaena leucocephala hedgerows + corn> hemerocallis fulva > corn> bare land, and decreased with increasing depth. the eigenvalues of soil infiltration were in the order of initial infiltrat ...201323755487
molecular analysis of a novel tandemly organized repetitive dna sequence in citrus limon (l.) burm.repetitive sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of repetitive dna sequences provide an insight into the organization of the genome of interest. here, we report the isolation and molecular analysis of a novel tandemly organized repetitive dna sequence from the genome of citrus limon. digestion of c. limon dna with hinf i produced a prominent fragment of approximately 300 bp. southern blotting revealed a ladder composed of ...200717666775
green synthesized silver nanoparticles for selective colorimetric sensing of hg2+ in aqueous solution at wide ph range.in the present study, the syntheses of crystalline silver and gold nanoparticles (nps) has been demonstrated in a green and environmentally friendly approach using citrus fruit extracts (lemon, citrus limon (cl-1) and sweet orange, citrus limetta (cl-2)). in addition, potentially hazardous metal ion sensing properties of these nps in aqueous solution has been explored. cl-1 and cl-2 that predominantly contained citric and ascorbic acid, exhibited different reducing abilities towards silver and g ...201323741735
bicarbonate blocks iron translocation from cotyledons inducing iron stress responses in citrus roots.the effect of bicarbonate ion (hco3(-)) on the mobilization of iron (fe) reserves from cotyledons to roots during early growth of citrus seedlings and its influence on the components of the iron acquisition system were studied. monoembryonic seeds of citrus limon (l.) were germinated "in vitro" on two iron-deprived media, supplemented or not with 10mm hco3(-) (-fe+bic and -fe, respectively). after 21d of culture, fe concentration in seedling organs was measured, as well as gene expression and en ...201323465471
authenticity control of essential oils containing citronellal and citral by chiral and stable-isotope gas-chromatographic analysis.enantioselective capillary gc on a supelco beta-dex 225 column (heptakis(2,3-di-o-acetyl-6-o-tert-butyldimethylsilyl)-beta-cyclodextrin spb 20poly--20% diphenyl, 80% dimethylsiloxane) and isotope-ratio mass spectrometry, coupled online with capillary gc on an hp5 column have been used for origin-specific analysis and authenticity control of essential oils, for example lemon (citrus limon), lemongrass (cymbopogon citratus and cymbopogon flexuosus), citronella (cymbopogon nardus l.--ceylon type an ...200617089103
green synthesis of gold nanoparticles using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) aqueous extract and its characterization.this study reports the biological synthesis of gold nanoparticles by the reduction of haucl(4) by using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) juice extract as the reducing and stabilizing agent. a various shape and size of gold nanoparticles were formed when the ratio of the reactants were altered with respect to 1.0mm chloroauric acid solution. the gold nanoparticles obtained were characterized by uv-visible spectra, transmission electron microscopy (tem) and x-ray ...201323211617
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