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chemical guide parameters for spanish lemon (citrus limon (l.) burm.) juices.to contribute for setting reference guideline for commercial juice extracted from the spanish lemon varieties, chemical composition of 92 direct and 92 reconstituted samples were investigated. in direct lemon juice, titratable acidity was 52.4 g/l, being the citric acid the main component. glucose, fructose and sucrose concentrations were 7.9, 7.3 and 4.5 g/l, respectively. predominant mineral was potassium (1264.2mg/l), followed by phosphorous (306 mg/l), calcium (112 mg/l) and magnesium (92.6 ...201424874375
ptrbam1, a β-amylase-coding gene of poncirus trifoliata, is a cbf regulon member with function in cold tolerance by modulating soluble sugar levels.β-amylase (bam) catalyses starch breakdown to generate maltose, which can be incorporated into sugar metabolism. however, the role of bam genes in cold tolerance is less characterized. in this study, we report the isolation and functional characterization of a chloroplast-localizing bam-encoding gene ptrbam1 from poncirus trifoliata. ptrbam1 was induced by cold, dehydration and salt, but repressed by maltose. overexpression of ptrbam1 in tobacco (nicotiana nudicaulis) increased bam activity, pro ...201424905016
in vitro/in vivo effect of citrus limon (l. burm. f.) juice on blood parameters, coagulation and anticoagulation factors in rabbits.the genus citrus of the family rutaceae includes many species e.g. citrus indica, citrus aurantifolia and citrus limon, among which citrus limon l. burm. f. has been reported to have highest antimicrobial activity. it is used as antidote against certain venom, due to its platelet inhibitory effect and also reported to have hypocholesterolemic effect. however its anticoagulant and thrombolytic effect were not been investigated, hence a prospective in-vitro/in-vivo study was designed to determine ...201425015459
utility of indels for species-level identification of a biologically complex plant group: a study with intergenic spacer in citrus.the consortium of barcode of life plant working group proposed to use the defined portion of plastid genes rbcl and matk either singly or in combination as the standard dna barcode for plants. but dna barcode based identification of biologically complex plant groups are always a challenging task due to the occurrence of natural hybridization. here, we examined the use of indels polymorphism in trnh-psba and trnl-trnf sequences for rapid species identification of citrus. dna from young leaves of ...201425048292
isolation and characterization of putative endophytic bacteria antagonistic to phoma tracheiphila and verticillium albo-atrum.a collection of 200 bacterial isolates recovered from citrus plants (citrus limon, citrus sinensis, and citrus reticulata), medicago truncatula and laurus nobilis, was established. in vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens phoma tracheiphila and verticillium albo-atrum. isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (pgpb) abilities, as well as for the presence of nonribosomal pe ...201425074353
molecular cloning and characterization of a geranyl diphosphate-specific aromatic prenyltransferase from lemon.prenyl residues confer divergent biological activities such as antipathogenic and antiherbivorous activities on phenolic compounds, including flavonoids, coumarins, and xanthones. to date, about 1,000 prenylated phenolics have been isolated, with these compounds containing various prenyl residues. however, all currently described plant prenyltransferases (pts) have been shown specific for dimethylallyl diphosphate as the prenyl donor, while most of the complementary dnas encoding these genes hav ...201425077796
in vitro biological evaluation of eight different essential oils against trypanosoma cruzi, with emphasis on cinnamomum verum essential oil.essential oils (eos) are complex mixtures of secondary metabolites from various plants. it has been shown that several eos, or their constituents, have inhibitory activity against trypanosomatid protozoa. thus, we analyzed the biological activity of different eos on trypanosoma cruzi, as well as their cytotoxicity on vero cells.201425148924
capturing of the monoterpene olefin limonene produced in saccharomyces cerevisiae.monoterpene olefins such as limonene are plant compounds with applications as flavouring and fragrance agents, as solvents and potentially also in polymer and fuel chemistry. we engineered baker's yeast saccharomyces cerevisiae to express a (-)-limonene synthase from perilla frutescens and a (+)-limonene synthase from citrus limon. both proteins were expressed either with their native plastid targeting signal or in a truncated form in which the plastidial sorting signal was removed. the yeast ho ...201525164098
adoption of exotic plants by an endemic australian weevil, orthorhinus cylindrirostris (coleoptera: curculionidae).the elephant weevil orthorhinus cylindrirostris (f.) (coleoptera: curculionidae) is endemic to australia and has colonized several exotic crops including citrus, blueberries, and grapevines. we compared the life history of this pest on two native and two exotic host plants: hickory wattle acacia falcata willdenow, rough-barked apple angophora floribunda (smith) sweet, lemon citrus limon (l.) burman, and blueberry vaccinium corymbosum (l.). blueberry was attacked more often (22 of the 24 plants) ...201425198945
salicylic acid and methyl jasmonate improve chilling tolerance in cold-stored lemon fruit (citrus limon).chilling injury (ci) is associated with the degradation of membrane integrity which can be aligned to phenolic oxidation activated by polyphenol oxidase (ppo) and peroxidase (pod), enzymes responsible for tissue browning. phenylalanine ammonia-lyase (pal) is a further enzyme prominent in the phenolic metabolism that is involved in acclimation against chilling stress. it was hypothesized that treatment with methyl jasmonate (mj) and salicylic acid (sa) may enhance chilling tolerance in lemon frui ...201425216124
behavioral effects of citrus limon in rats.anxiety and depression are increasing worldwide, however these disorders may be managed by making healthier changes is dietary pattern, since there are evidences that diet rich in antioxidants and vitamins help reduce anxiety and depression. hence present study was designed to evaluate the behavioral effects of citrus limon in rats at three different doses i.e. 0.2, 0.4 and 0.6 ml/kg considered as low, moderate and high doses. anxiolytic and antidepressant activities were specifically assessed t ...201525227172
toxic effect of citrus peel constituents on anastrepha fraterculus wiedemann and ceratitis capitata wiedemann immature stages.the toxicity of essential oils from the citrus peel has been proposed as the major resistance mechanism offered by citrus to fruit fly infestation. we evaluated the insecticidal activity of the ether extracts from the lemon (citrus limon [l.] burm.) and grapefruit (c. paradisi macfadyen) peel as well as from limonene and citral against anastrepha fraterculus (wiedemann) and ceratitis capitata (wiedemann) immature stages. we also evaluated the toxicity of the extracts at two ripening stages. extr ...201425237738
phospholipases a2: enzymatic assay for snake venom (naja naja karachiensis) with their neutralization by medicinal plants of pakistan.phospholipases a2 (pla2) are the most lethal and noxious component of naja naja karachiensis venom. they are engaged to induce severe toxicities after their penetration in victims. present study was designed to highlight hydrolytic actions of pla. in an egg yolk mixture and to encounter their deleterious effects via medicinal plants of pakistan. pla2 were found to produce free fatty acids in a dose dependent manner. venom at concentration of 0.1 mg was found to liberate 26.6 pmoles of fatty acid ...201425272888
beverages of lemon juice and exotic noni and papaya with potential for anticholinergic effects.lemon (citrus limon (l.) burm. f.) juice beverages enriched either with noni (morinda citrifolia l.) (ln) or papaya (carica papaya l.) (lp), were characterized by hplc-dad-esi/ms(n), the antioxidant capacity was evaluated by (dpph·), superoxide (o2(·-)), hydroxyl radicals (·oh) and hypochlorous acid (hocl) assays, and their potential as acetylcholinesterase (ache) and butyrylcholinesterase (buche) inhibitors was also assessed. the fruits are rich in a wide range of bioactive phenolics. regarding ...201525306312
a survey of plants responsible for causing irritant contact dermatitis in the amathole district, eastern cape, south africa.potentially harmful plants grow almost everywhere, hence, it is not practical to eradicate them all. however, a basic understanding of adverse cutaneous reactions and the common plants that cause each type can enable vulnerable individuals to discover the source of their dermatitis and thus prevent re-exposure. the aim of this study therefore, was to document the plants responsible for irritant contact dermatitis (icd) in the eastern cape, along with their respective irritants and clinical prese ...201425311272
memory boosting effect of citrus limon, pomegranate and their combinations.memory is greatly influenced by factors like food, stress and quality of sleep, hence present study was designed to evaluate the effect of citrus limon and pomegranate juices on memory of mice using harvard panlab passive avoidance response apparatus controlled through le2708 programmer. passive avoidance is fear-motivated tests used to assess short or long-term memory of small animals, which measures latency to enter into the black compartment. animals at mcld showed highly significant and sign ...201425362607
traditional phytoremedies for the treatment of menstrual disorders in district udhampur, j&k, india.herbal remedies form an integral part of healing and are considered to be the oldest forms of health care known to mankind. the present study aims to document traditional phytoremedies for the treatment of menstrual disorders in udhampur district of j&k, india.201525479156
parasitoids and hyperparasitoids (hymenoptera) on aphids (hemiptera) infesting citrus in east mediterranean region of turkey.the aphids, aphid parasitoids, and hyperparasitoids found in citrus orchards, the parasitoids' and hyperparasitoids' seasonal abundance, and the plant-aphid-parasitoid relationships in hatay, osmaniye, adana, and mersin provinces of the east mediterranean region of turkey are presented in the present 2-yr study. aphidius colemani viereck, binodoxys angelicae (haliday), and lysiphlebus confusus tremblay and eady (hymenoptera: braconidae: aphidiinae) were encountered as the most common parasitoids ...201425480969
the use of aromasticks at a cancer centre: a retrospective audit.to consider the use of aromasticks in a cancer centre in the uk: the reasons for their use, the choice of essential oils used in them and the demographics of the patients to whom they were given.201425486854
identification, synthesis, and characterization of novel sulfur-containing volatile compounds from the in-depth analysis of lisbon lemon peels (citrus limon l. burm. f. cv. lisbon).lemons (citrus limon) are a desirable citrus fruit grown and used globally in a wide range of applications. the main constituents of this sour-tasting fruit have been well quantitated and characterized. however, additional research is still necessary to better understand the trace volatile compounds that may contribute to the overall aroma of the fruit. in this study, lisbon lemons (c. limon l. burm. f. cv. lisbon) were purchased from a grove in california, usa, and extracted by liquid-liquid ex ...201525639384
involvement of an extracellular fungus laccase in the flavonoid metabolism in citrus fruits inoculated with alternaria alternata.fungi of the genus alternaria are responsible for substantial pre-harvest losses in citrus. in this study a degradative metabolism of flavonoids (flavanones, flavones and polymethoxyflavones) was observed when 'fortune' mandarin, citrus limon and citrus paradisi, fruits were inoculated with alternaria alternata, a pre-harvest pathogenic fungus. associated to this flavonic metabolism the de novo synthesis of the phytoalexin scoparone was detected. this metabolism of flavonoids is caused by an ext ...201525686700
chemical composition of different botanical origin honeys produced by sicilian black honeybees (apis mellifera ssp. sicula).in 2008 a slow food presidium was launched in sicily (italy) for an early warning of the risk of extinction of the sicilian native breed of black honeybee (apis mellifera l. ssp sicula). today, the honey produced by these honeybees is the only sicilian honey produced entirely by the black honeybees. in view of few available data regarding the chemical composition of a. mellifera ssp. sicula honeys, in the present investigation the chemical compositions of sulla honey (hedysarum coronarium l.) an ...201525730368
systematic hplc/esi-high resolution-qtof-ms methodology for metabolomic studies in nonfluorescent chlorophyll catabolites pathway.characterization of nonfluorescent chlorophyll catabolites (nccs) and dioxobilane-type nonfluorescent chlorophyll catabolite (dncc) in peel extracts of ripened lemon fruits (citrus limon l.) was performed by hplc/esi-high resolution-qtof-ms method. compounds were identified in samples on the basis of measured accurate mass, isotopic pattern, and characteristic fragmentation profile with an implemented software postprocessing routine. three ncc structures already identified in other vegetal tissu ...201525741450
fcwrky70, a wrky protein of fortunella crassifolia, functions in drought tolerance and modulates putrescine synthesis by regulating arginine decarboxylase gene.wrky comprises a large family of transcription factors in plants, but most wrky members are still poorly understood. in this study, we report functional characterization of a group iii wrky gene (fcwrky70) from fortunella crassifolia. fcwrky70 was greatly induced by drought and abscisic acid, but slightly or negligibly by salt and cold. overexpression of fcwrky70 in tobacco (nicotiana nudicaulis) and lemon (citrus lemon) conferred enhanced tolerance to dehydration and drought stresses. transgeni ...201525808564
citrus limon-derived nanovesicles inhibit cancer cell proliferation and suppress cml xenograft growth by inducing trail-mediated cell death.nanosized vesicles are considered key players in cell to cell communication, thus influencing physiological and pathological processes, including cancer. nanovesicles have also been found in edible-plants and have shown therapeutic activity in inflammatory bowel diseases; however information on their role in affecting cancer progression is missing.our study identify for the first time a fraction of vesicles from lemon juice (citrus limon l.), obtained as a result of different ultracentrifugation ...201526098775
identification of hop stunt viroid infecting citrus limon in china using small rnas deep sequencing approach.the advent of next generation sequencing technology has allowed for significant advances in plant virus discovery, particularly for identification of covert viruses and previously undescribed viruses. the citrus limon burm. f. (c. limon) is a small evergreen tree native to asia, and . china is the world's top lemon-producing nation.201526148502
antimicrobial potential of the ethiopian thymus schimperi essential oil in comparison with others against certain fungal and bacterial species.to evaluate the in vitro activities of ethiopian thymus schimperi with other three hydro distilled essential oils against dermatophytes (tricophyton spp. and microsporum spp.) and other pathogenic micro organisms.201526227483
antifungal effect of essential oils against fusarium keratitis isolates.the present study was carried out to investigate the antifungal effects of cinnamomum zeylanicum, citrus limon, juniperus communis, eucalyptus citriodora, gaultheria procumbens, melaleuca alternifolia, origanum majorana, salvia sclarea, and thymus vulgaris essential oils against fusarium species, the most common etiologic agents of filamentous fungal keratitis in south india. c. zeylanicum essential oil showed strong anti-fusarium activity, whereas all the other tested essential oils proved to b ...201526227503
an ethnobotanical survey of medicinal plants in trinidad.an ethnobotanical survey was conducted on the caribbean island of trinidad to identify medicinal plants commonly used in traditional medicine to treat a variety of medical conditions.201526369926
"singing in the tube"--audiovisual assay of plant oil repellent activity against mosquitoes (culex pipiens).plant essential oils have been suggested as a promising alternative to the established mosquito repellent deet (n,n-diethyl-meta-toluamide). searching for an assay with generally available equipment, we designed a new audiovisual assay of repellent activity against mosquitoes "singing in the tube," testing single mosquitoes in drosophila cultivation tubes. statistics with regression analysis should compensate for limitations of simple hardware. the assay was established with female culex pipiens ...201626412058
pathogen infection drives patterns of nutrient resorption in citrus plants.nutrient resorption processes in the plants infected by pathogen remain poorly understood. huanglongbing (hlb) is a destructive disease of citrus. hlb-pathogen 'candidatus liberibacter asiaticus' grows specifically in the phloem of hosts and may cause problems in the plant vascular system after infection. therefore, it brings a great concern about the phloem nutrient transport and nutrient intra-cycling in hlb-affected plants. we investigated the effects of 'ca. l. asiaticus' infection on nitrog ...201526419510
beneficial effect of citrus limon peel aqueous methanol extract on experimentally induced urolithic rats.citrus limon (l.) burm.f. (rutaceace) is a commonly available fruit variety with high medicinal and industrial values.201626452728
development and reproduction of panonychus citri (prostigmata: tetranychidae) on different species and varieties of citrus plants.the species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite panonychus citri (mcgregor) (prostigmata: tetranychidae) and alter the pest management programs in citrus groves. in this study we evaluated, in the laboratory, the development and reproduction of p. citri and estimated its life table parameters when reared on four varieties of citrus sinensis (l.) osbeck (valencia, pera, natal, and hamlin), one variety of citrus reticulata ...201526459376
differential tolerance of 3 self-rooted citrus limon cultivars to nacl stress.one-year-old self-rooted cuttings of three citrus limon cultivars (nouvel athos, lisbon, maglini) were grown in 1 l black plastic bags, containing a mixture of sand: perlite (1:1), in order to investigate: i) if genotypic differences to salt stress existed, ii) if kno3 can alleviate salinity stress, iii) the role of carbohydrates (such as the sugars fructose, glucose and sucrose) and proline as possible osmoregulators in c. limon osmoprotection, and iv) if genotypic differences to salt stress to ...201526476793
characterization of lemon (citrus limon) polar extract by liquid chromatography-tandem mass spectrometry in high resolution mode.eighty four metabolites (32 flavonoids, 15 amino acids, nine carboxylic acids, six coumarins, six sugars, five phenolic acids and 11 unclassified compounds) have been tentatively identified in a polar extract from lemon, without reference standards, based on their liquid chromatography-quadrupole-time-of-flight ms/ms spectra and the comparison with databases. despite information in databases for some families of plant compounds is poor, tentative identification based on ms/ms information (mass o ...201526505764
tissue-specific production of limonene in camelina sativa with the arabidopsis promoters of genes banyuls and fruitfull.arabidopsis promoters of genes banyuls and fruitfull are transcribed in camelina. they triggered the transcription of limonene synthase and induced higher limonene production in seeds and fruits than camv 35s promoter. camelina sativa (camelina) is an oilseed crop of relevance for the production of biofuels and the plant has been target of a recent and intense program of genetic manipulation aimed to increase performance, seed yield and to modify the fatty acid composition of the oil. here, we h ...201626530959
citrus limon extract: possible inhibitory mechanisms affecting testicular functions and fertility in male mice.the effect of oral administration of 50% ethanolic leaf extract of citrus limon (500 and 1,000 mg/kg body weight/day) for 35 days on fertility and various male reproductive endpoints was evaluated in parkes strain of mice. testicular indices such as histology, 3β- and 17β-hsd enzymes activity, immunoblot expression of star and p450scc, and germ cell apoptosis by tunel and casp- 3 expression were assessed. motility, viability, and number of spermatozoa in the cauda epididymidis, level of serum te ...201626787324
comparison of heat and mass transfer of different microwave-assisted extraction methods of essential oil from citrus limon (lisbon variety) peel.dried and fresh peels of citrus limon were subjected to microwave-assisted hydrodistillation (mahd) and solvent-free microwave extraction (sfme), respectively. a comparison was made between mahd and sfme with the conventional hydrodistillation (hd) method in terms of extraction kinetic, chemical composition, and antioxidant activity. higher yield results from higher extraction rates by microwaves and could be due to a synergy of two transfer phenomena: mass and heat acting in the same way. gas c ...201526788292
phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates.the search for new antimicrobial compounds is ongoing. its importance cannot be overemphasized in an era of emerging resistant pathogenic organisms. this study therefore investigated the phytochemical composition and antioxidant and antimicrobial activities of different citrus juice concentrates. fruit juices of citrus tangerine (tangerine), citrus paradisi (grape), citrus limon (lemon), and citrus aurantifolia (lime) were evaluated. antimicrobial activities against five bacterial and three fung ...201626788316
comparative study of the effect of sample pretreatment and extraction on the determination of flavonoids from lemon (citrus limon).flavonoids have shown to exert multiple beneficial effects on human health, being also appreciated by both food and pharmaceutical industries. citrus fruits are a key source of flavonoids, thus promoting studies to obtain them. characteristics of these studies are the discrepancies among sample pretreatments and among extraction methods, and also the scant number of comparative studies developed so far.201626807979
flavor identification and intensity: effects of stimulus context.two experiments presented oral mixtures containing different proportions of the gustatory flavorant sucrose and an olfactory flavorant, either citral (experiment 1) or lemon (experiment 2). in 4 different sessions of each experiment, subjects identified each mixture as "mostly sugar" or "mostly citrus/lemon" or rated the perceived intensities of the sweet and citrus components. different sessions also presented the mixtures in different contexts, with mixtures containing relatively high concentr ...201626830499
plants with potential use on obesity and its complications.obesity is the most prevalent nutritional disease and a growing public health problem worldwide. this disease is a causal component of the metabolic syndrome related with abnormalities, including hyperglycemia, dyslipidemia, hypertension, inflammation, among others. there are anti-obesity drugs, affecting the fundamental processes of the weight regulation; however they have shown serious side effects, which outweigh their beneficial effects. most recent studies on the treatment of obesity and it ...201526869866
effects of 20 selected fruits on ethanol metabolism: potential health benefits and harmful impacts.the consumption of alcohol is often accompanied by other foods, such as fruits and vegetables. this study is aimed to investigate the effects of 20 selected fruits on ethanol metabolism to find out their potential health benefits and harmful impacts. the effects of the fruits on ethanol metabolism were characterized by the concentrations of ethanol and acetaldehyde in blood, as well as activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in liver of mice. furthermore, potential hea ...201627043608
chemical characterization of citrus limon var. pompia and incorporation in phospholipid vesicles for skin delivery.the components of pompia, a hybrid citrus species cultivated only in sardinia (italy), were extracted using an environmentally-friendly method and food-grade solvents. taking into account that only few data are available on pompia composition, the phytochemical fingerprint of its rind extract was obtained by accurate component separation and identification, combining hplc and mass spectrometry. different flavones such as naringin (23.77μg/mg), neoeriocitrin (46.53μg/mg) and neohesperidin (44.57μ ...201627084291
in vitro and in vivo effect of citrus limon essential oil against sarcoptic mange in rabbits.the effect of lemon oil (citrus limon) on sarcoptes scabiei var. cuniculi was evaluated in vitro and in vivo. the mite samples were collected from naturally infected rabbits. the lemon oil was prepared in six concentrations by dilution with distilled water (2.5, 5, 10, 20, 50, and 100 %). in vitro application was done in five replicates for each concentration in petri dishes in the laboratory. the treated mites were observed at 1, 12, and 24 h post application (pa) for lemon oil effect. in addit ...201627098160
chemical profile and antioxidant properties of extracts and essential oils from citrus × limon (l.) burm. cv. femminello comune.citrus × limon cv. femminello comune (rutaceae) from rocca imperiale (italy), one of the six protected geographical indication (pgi) italian lemon crops, has been recently received renewed interest. in this work, fresh and dried peels and leaves were extracted by hydrodistillation, supercritical fluid extraction (sfe), and soxhlet apparatus. chemical profile was assessed by gas chromatography (gc) and gas chromatography/mass spectrometry (gc/ms). except for leaves extracts obtained by soxhlet ap ...201627112122
effect of sample pretreatment on the extraction of lemon (citrus limon) components.a study on the key role of lemon sample pretreatment on the analytical results is here presented. the objective of the study was to analyze the differences between extracts obtained from lyophilized and air-dried samples-the most common sample pretreatment in citrus studies-in comparison to extracts from fresh samples. all the extracts were obtained with ultrasound assistance and analyzed by lc-qtof ms/ms. the dataset, constituted by 74 tentative identified metabolites, was first evaluated by an ...201627130132
efficacy of a nasal spray from citrus limon and cydonia oblonga for the treatment of hay fever symptoms-a randomized, placebo controlled cross-over study.nasal spray from lemon and quince (lqns) is used to treat hay fever symptoms and has been shown to inhibit histamine release from mast cells in vitro. forty-three patients with grass pollen allergy (gpa) were randomized to be treated either with placebo or lqns for one week, respectively, in a cross-over study. at baseline and after the respective treatments patients were provoked with grass pollen allergen. outcome parameters were nasal flow measured with rhinomanometry (primary), a nasal sympt ...201627282490
traceability of 'limone di siracusa pgi' by a multidisciplinary analytical and chemometric approach.food traceability is increasingly relevant with respect to safety, quality and typicality issues. lemon fruits grown in a typical lemon-growing area of southern italy (siracusa), have been awarded the pgi (protected geographical indication) recognition as 'limone di siracusa'. due to its peculiarity, consumers have an increasing interest about this product. the detection of potential fraud could be improved by using the tools linking the composition of this production to its typical features. th ...201627283690
the in vitro effect of selected essential oils on the growth and mycotoxin production of aspergillus species.the aim of the present study was to assess the antifungal and anti-toxinogenic activity of 15 essential oils (eos) against three fungi of the genus aspergillus (a. parasiticus kmi-227-lr, a. parasiticus kmi-220-lr and a. flavus kmi-202-lr). the minimum inhibitory doses (mids) of the tested essential oils and their antifungal activity were determined using the micro-atmosphere method. the original commercial essential oil samples of jasminum officinale l., thymus vulgaris l., syzygium aromaticum ...201627322876
traditional alcoholic beverages and their value in the local culture of the alta valle del reno, a mountain borderland between tuscany and emilia-romagna (italy).traditional alcoholic beverages (tabs) have only received marginal attention from researchers and ethnobotanists so far, especially in italy. this work is focused on plant-based tabs in the alta valle del reno, a mountainous area on the border between tuscany and emilia-romagna regions. the aims of our study were to document local knowledge about tabs and to analyze and discuss the distribution of related knowledge within the investigated communities.201627333772
the mir396b of poncirus trifoliata functions in cold tolerance by regulating acc oxidase gene expression and modulating ethylene-polyamine homeostasis.micrornas (mirnas) are non-coding regulatory molecules that play important roles in a variety of biological processes. although a number of cold-responsive mirnas have been computationally identified, functions and mechanisms of most mirnas are not well understood. herein, the function of trifoliate orange [poncirus trifoliata (l.) raf.] mirna396b (ptr-mir396b) in cold tolerance and its potential regulatory module were investigated. compared with the wild type (wt), transgenic lemon (citrus limo ...201627402968
behavioral effects of citrus limon and punica granatum combinations in rats.dietary supplements are becoming more influential as viable treatment for common chronic diseases and to promote normal development and functions of all system including brain. disorders like anxiety and depression may be managed through healthier variations is dietary pattern, since there are indications that diet rich in antioxidants and vitamins diminish anxiety and depression. hence this investigation was planned to assess the behavioral effects of citrus limon and punica granatum in two com ...201727510713
the protective effect of citrus limon essential oil on hepatotoxicity and nephrotoxicity induced by aspirin in rats.citrus limon is a member of the large rutaceae family characterized by its therapeutic proprieties and has been widely used in traditional medicine to treat various diseases. this study investigates the protective effect of citrus limon essential oil against a high dose of aspirin-induced acute liver and kidney damage in female wistar albino rats. twenty-eight adult female wistar rats were divided into 4 groups of 7 each: (1) a control group; (2) a group of rats which was kept untreated for 56da ...201627571876
anti-proliferative or cytopathic potential of thapsia garganica, citrus sinensis, citrus limon and vinca rosea extracts against human embryonic kidney carcinoma cell line.developing anti-cancer drugs from natural products is receiving increasing interest worldwide due to limitations and side-effects of anti-cancer drugs. the purpose of this study was to explore the anti-proliferative or cytopathic potential of natural compounds derived from plant sources as alternatives of synthetic compounds on human embryonic kidney carcinoma (hek) cell line.201627618522
resistance to citrus canker induced by a variant of xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes.xanthomonas citri ssp. citri (x. citri) is the causal agent of asiatic citrus canker, a disease that seriously affects most commercially important citrus species worldwide. we have identified previously a natural variant, x. citri a(t) , that triggers a host-specific defence response in citrus limon. however, the mechanisms involved in this canker disease resistance are unknown. in this work, the defence response induced by x. citri a(t) was assessed by transcriptomic, physiological and ultrastr ...201627647752
metabolomic analysis of primary metabolites in citrus leaf during defense responses.mechanical damage is one of the unavoidable environmental stresses to plant growth and development. plants induce a variety of reactions which defend against natural enemies and/or heal the wounded sites. jasmonic acid (ja) and salicylic acid (sa), defense-related plant hormones, are well known to be involved in induction of defense reactions and play important roles as signal molecules. however, defense related metabolites are so numerous and diverse that roles of individual compounds are still ...201727789172
by-product identification and phytotoxicity of biodegraded direct yellow 4 dye.citrus limon peroxidase mediated decolourization of direct yellow 4 (dy4) was investigated. the process variables (ph, temperature, incubation time, enzyme dose, h2o2 amount, dye concentration, co-metal ions and surfactants) were optimized for maximum degradation of dye. maximum dye decolourization of 89.47% was achieved at ph 5.0, temperature 50 °c, enzyme dose 24 u/ml, h2o2 concentration 0.25 mm and dy4 concentration 18.75 mg/l and incubation time 10 min. the co-metal ions and surfactants did ...201727889513
traditional mediterranean plants: characterization and use of an essential oils mixture to treat malassezia otitis externa in atopic dogs.several plants extracts from mediterranean countries are traditionally employed in skin troubles both in humans and in animals. malassezia pachydermatis is a lipophylic yeast responsible for otitis externa and dermatitis in dogs and for cutaneous and systemic disease in humans. five mixtures of essential oils obtained from mediterranean plants (citrus paradisi, salvia sclarea, ocimum basilicum, rosmarinus officinalis, citrus limon, anthemis nobilis, lavandula hybrida and thymus vulgaris) provide ...201627917678
diosmetin triggers cell apoptosis by activation of the p53/bcl-2 pathway and inactivation of the notch3/nf-κb pathway in hepg2 cells.diosmetin (dios), a flavonoid compound, is abundant in citrus limon. emerging studies have shown that dios is an effective compound implicated in multiple types of cancer. however, whether dios serves a role in hepatocellular carcinoma (hcc) is still obscure. hepg2 cells were used in the present study, and it was observed that dios exhibited antitumor activity against liver cancer cells. western blotting was performed to evaluate cell apoptosis and survival-associated proteins, and the results d ...201628101238
protective effects of essential oil of citrus limon against aspirin-induced toxicity in iec-6 cells.aspirin, one of the widely used nonsteroidal anti-inflammatory drugs, is the most highly consumed pharmaceutical product in the world. however, it has several side effects in cells. this study was designed to investigate the antioxidative activity and cytoprotective effects of essential oil of citrus limon (eoc) extracted from leaves against aspirin-induced damages in the rat small intestine epithelial cells (iec-6). biochemical indicators were used to assess cytotoxicity and oxidative damages c ...201628177735
experimental evidence and in silico identification of tryptophan decarboxylase in citrus genus.plant tryptophan decarboxylase (tdc) converts tryptophan into tryptamine, precursor of indolealkylamine alkaloids. the recent finding of tryptamine metabolites in citrus plants leads to hypothesize the existence of tdc activity in this genus. here, we report for the first time that, in citrus x limon seedlings, deuterium labeled tryptophan is decarboxylated into tryptamine, from which successively deuterated n,n,n-trimethyltryptamine is formed. these results give an evidence of the occurrence of ...201728208655
anti-mycobacteria potential and synergistic effects of combined crude extracts of selected medicinal plants used by bapedi traditional healers to treat tuberculosis related symptoms in limpopo province, south africa.tuberculosis is an infectious communicable disease and the causative agent of the disease has over the years developed resistance to streamline chemotherapeutic agents with dire consequences and there is a need for development of new and more potent alternatives.201728235402
antidiarrheal activity of hexane extract of citrus limon peel in an experimental animal model.acute diarrhea is one of the major illnesses that cause death in children, despite clinical interventions and the use of oral rehydration therapy. thus, there is need to discover other effective, affordable and accessible treatments for this disease. therefore, this study was carried out to investigate the effects of hexane extract of citrus limon peel (heclp) on castor oil-induced diarrhea in rats.201728285621
ecophysiology of cuticular transpiration: comparative investigation of cuticular water permeability of plant species from different habitats.water permeabilities of astomatous, isolated cuticular membranes (cm) of 24 different plants species were measured. permeances varied from 1.7×10(-11) m·s(-1) (vanilla planifolia leaf) up to 2.1×10(-9) m·s(-1) (malus cf. domestica fruit) among different plant species, thus covering a range of over 2 orders of magnitude. ranking of species according to permeances resulted in four distinct groups. the first group, of species with the lowest cuticular transpiration rates, included evergreen species ...199628307383
dietary dehydrated lemon peel improves the immune but not the antioxidant status of gilthead seabream (sparus aurata l.).lemon (citrus limon) is the third most important species of citrus in the world, while spain is the major producer in europe. numerous beneficial effects of lemon are known, which explains their use in traditional medicine. the paper describes the effect of dietary dehydrated lemon peel (a sub-product of the lemon industry) on the growth, immune and antioxidant status of gilthead seabream (sparus aurata l.) over a period of 30 days. fish fed diets enriched with dehydrated lemon peel (1.5% and 3% ...201728359944
qualitative and nutraceutical aspects of lemon fruits grown on the mountainsides of the mount etna: a first step for a protected designation of origin or protected geographical indication application of the brand name 'limone dell'etna'.the cultivation of lemon (citrus limon (l.) burm f.) has occurred for centuries at the mountainsides of the mount etna the largest active volcano in europe (sicily, italy). the peculiar geographical, soil and climatic conditions that characterize this area have recently prompted citrus growers to launch the brand name 'limone dell'etna' for the lemon fruits produced in that area. the present research focused on evaluating the quality and nutraceutical properties of the 'limone dell'etna' fruit j ...201528411990
characterization of phenolic profile and antioxidant capacity of different fruit part from lemon (citrus limon burm.) cultivars.phenolic composition and antioxidant capacity of different fruit part including peel, pulp, juice, whole fruit and seed from five lemon cultivars (feiminailao, cuningmeng limeng, pangdelusaningmeng, beijingningmeng) were investigated. caffeic acid (9.31-741.4 μg/g fw) and chlorogenic acid (2.7-527.5 μg/g fw) were the dominant phenolic acid in fruit tested, pangdelusaningmeng (pd) and limeng peels with the highest contents, respectively. hesperidin was the predominant flavanone (10.27-3315 μg/g f ...201728416860
determination of volatile flavour profiles of citrus spp. fruits by sde-gc-ms and enantiomeric composition of chiral compounds by mdgc-ms.citrus fruits are known to have characteristic enantiomeric key compounds biosynthesised by highly stereoselective enzymatic mechanisms. in the past, evaluation of the enantiomeric ratios of chiral compounds in fruits has been applied as an effective indicator of adulteration by the addition of synthetic compounds or natural components of different botanical origin.028444796
antibiofilm effects of citrus limonum and zingiber officinale oils on biofilm formation of klebsiella ornithinolytica, klebsiella oxytoca and klebsiella terrigena species.microbial cells growing in biofilms, play a huge role in the spread of antimicrobial resistance. in this study, biofilm formation of klebsiella strains belonging to 3 different klebsiella species (k. ornithinolytica, k. oxytoca and k. terrigena), cooccurences' effect on biofilm formation amount and anti-biofilm effects of citrus limon and zingiber officinale essential oils on biofilm formations of highest biofilm forming k. ornithinolytica, k. oxytoca and k. terrigena strains were determined.201628480361
ethnobotanical survey of medicinal plants used as insects repellents in six malaria endemic localities of cameroon.the combined efforts to combat outdoor/indoor transmission of malaria parasites are hampered by the emerging vector resistance in a wide variety of malaria-endemic settings of africa and the rest of the world, stressing the need for alternative control measures. this study aimed at documenting insect's repellent plant species used by indigenous populations of 6 localities of east, south, west and centre regions of cameroon.201728595645
structures of antimutagenic constituents in the peels of citrus limon.the methanolic extracts from the peels of citrus limon were found to show antimutagenic effects against 3-amino-1,4-dimethyl-5h-pyrido[4,3-b]indole, and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) in the ames test. from the methanolic extracts, four new coumarins (wakayamalimonol a-d) and a new furanocoumarin (wakayamalimonol e) were isolated together with fifteen known compounds. the absolute stereostructures of the new compounds were determined by chemical synthesis and the modified ...201728699128
cold pressed versus solvent extracted lemon (citrus limon l.) seed oils: yield and properties.during the processing of lemon fruit, a large quantity of seeds is produced as a by-product. these seeds contain valuable components; therefore, required to be evaluated. this study aimed to compare the cold pressed with hexane-extracted lemon seed oils and determine their physicochemical and thermal properties. cold pressing yielded significantly lower oil (36.84%) than hexane extraction (71.29%). in addition, the concentrations of free fatty acids, peroxides, and p-anisidine were lower in the ...201728720945
citrus lemon essential oil: chemical composition, antioxidant and antimicrobial activities with its preservative effect against listeria monocytogenes inoculated in minced beef meat.lemon (citrus limon) is a flowing plant belonging to the rutaceae family. citrus plants constitute one of the main valuable sources of essential oil used in foods and medicinal purposes.201728774297
antimicrobial synergism and cytotoxic properties of citrus limon l., piper nigrum l. and melaleuca alternifolia (maiden and betche) cheel essential oils.the chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of citrus limon (lemon), piper nigrum (green pepper) and melaleuca alternifoila (tea tree) essential oils (eos) were investigated.201728815601
two of a kind but different: luminescent carbon quantum dots from citrus peels for iron and tartrazine sensing and cell imaging.citrus sinensis and citrus limon peels were used to synthesize two different kinds of carbon quantum dots (cqds) via an unsophisticated and inexpensive carbonization procedure. the proposed synthesis is straightforward and adheres to the principles of green chemistry since no organic solvents are used and no toxic by-products are formed, while the residual resources employed facilitate the large scale synthesis of dots. the citrus sinensis and citrus limon peels are proved to be excellent precur ...201728841995
dietary euphorbia hirta extract improved the resistance of sharptooth catfish clarias gariepinus to aeromonas hydrophila.aqueous and methanol extracts of lemon citrus limon peel, euphorbia hirta (aerial parts), and fenugreek trigonella foenum-graecum seeds were tested for their in vitro antimicrobial activities against the bacterium aeromonas hydrophila. a swab paper disk method showed that the methanol extract of e. hirta (ehe) had the largest inhibition zone and the lowest minimal inhibitory concentration compared to all other herbal extracts. based on these results, ehe was included in the diets of sharptooth c ...201728937913
a novel citrus viroid found in australia, tentatively named citrus viroid vii.a novel citrus viroid was discovered in a non-symptomatic lisbon lemon (citrus x limon l. burm.f.) tree in new south wales, australia. bioindexing, molecular detection and characterization involving sequencing combined with in silico analysis for the identification of the viroid-rna hallmark properties of transmissibility and autonomous replication as well as specific sequence and structural motifs suggest that this viroid is a member of a new species in the genus apscaviroid, family pospiviroid ...201829027592
label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-coa carboxylase alpha as antitumor target of citrus limon-derived nanovesicles.we have previously isolated exosome-like nanoparticles from citrus-limon juice, able to inhibit in vitro and in vivo tumor cell growth. in order to deeply understand the mechanism underlying nanovesicle effects, we performed a proteomic profile of treated colorectal cancer cells. among the proteins differentially expressed after nanovesicle treatment, we found a significant downregulation of the acetyl-coa carboxylase 1 (acaca) and we demonstrated that silencing acaca in cancer cells leads to a ...201829197582
metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium synechocysti s sp. pcc 6803.isoprenoids are diverse natural compounds, which have various applications as pharmaceuticals, fragrances, and solvents. the low yield of isoprenoids in plants makes them difficult for cost-effective production, and chemical synthesis of complex isoprenoids is impractical. microbial production of isoprenoids has been considered as a promising approach to increase the yield. in this study, we engineered the model cyanobacterium synechocystis sp. pcc 6803 for sustainable production of a commercial ...201729235513
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