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properties of a fructose-1,6-diphosphate-activated lactate dehydrogenase from acholeplasma laidlawii type a.acholeplasma laidlawii a possesses a nicotinamide adenine dinucleotide (nad)-dependent l(+)-lactate dehydrogenase (ldh) which is activated specifically by low concentrations of fructose-1, 6-diphosphate (fdp). studies with partially purified enzyme show that the kinetic response to fdp is hyperbolic. the enzyme is inhibited by inorganic phosphate, adenosine triphosphate, and high concentrations of reduced nad (nadh). low activity is demonstrable in the absence of fdp at ph 6.0 to 7.2, but fdp is ...19734712565
the effect of alterations in fatty acid composition and cholesterol content on the nonelectrolyte permeability of acholeplasma laidlawii b cells and derived liposomes. 19734719142
studies of sugar transport in acholeplasma laidlawii. 19734772677
selective simultaneous proton magnetic resonance labelling of hydrophilic and hydrophobic regions of phospholipid vesicles. effect of polar and apolar reagents. 19734795847
isolation and purification of tdna from mycoplasma. 19734803254
isolation of mycoplasmatales virus-laidlawii 3, a new virus infecting acholeplasma laidlawii. 19734736684
further studies on the morphology and composition of mycoplasmatales virus-laidlawii 2. 19734735309
incorporation of 3h-uridine and 3h-uracil into rna: a simple technique for the detection of mycoplasma contamination of cultured cells. 19744817725
intracellular replication of mycoplasmavirus mvl51.the intracellular replication of mvl51, a group l1 mycoplasmavirus, was investigated. the single-stranded parental dna was found to enter the cell and become converted to double-stranded dna. this replicated to yield additional double-stranded dna molecules. the parental viral dna was found to leave the replication complex and become associated with large molecular weight dna not involved with viral replication. progeny viral dna formed from the double-stranded dna and an intracellular accumulat ...19744821487
interaction of mycoplasma with viruses. i. primary adsorption of virus is ionic in mechanism.we have studied the adsorption of the gourlay acholeplasma virus mvl-1 to the host cell acholeplasma laidlawii ja-1. successful adsorption depends primarily on some unknown action of the serum factor in the medium, but evaluation of various physical parameters indicates clearly that given this factor the kinetics is pseudo first order (k = 3 x 10(-9) cm(3)/min) and the mechanism ionic. chiefly important are the ionic strength of the cation and ph (optimal na(+) = 0.08 m, ph = 6). the system seem ...19744824711
the effect of alterations in the physical state of the membrane lipids on the ability of acholeplasma laidlawii b to grow at various temperatures. 19744830862
effect of fatty acyl chain length on some structural and functional parameters of acholeplasma membranes. 19744844283
isolation of acholeplasma laidlawii from guinea-pigs. 19744852593
[identification of mycoplasma and bacterial l forms by polyacrylamide gel electrophoresis]. 19744598211
polyene antibiotic-sterol interactions in membranes of acholeplasma laidlawii cells and lecithin liposomes. 3. molecular structure of the polyene antibiotic-cholesterol complexes. 19744546885
isolation of acholeplasma laidlawii from commercial, serum-free tissue culture medium and studies on its survival and detection.this report documents the first isolation of acholeplasma laidlawii from a commercial lot of serum-free dulbecco basal medium. experimental studies demonstrated survival of the organism for at least 1 year depending on the concentration of the contaminating organism as well as ph and temperature of storage of the serum-free medium. a comparison of isolation methods showed that concentration by filtration through 220-nm membrane filters and testing the filters for mycoplasma recovery, especially ...19744598432
epidemiologic observations on mycoplasma mastitis. 19744602678
effect of mycoplasma dispar, mycoplasma bovirhinis, acholeplasma laidlawii and t-mycoplasmas on explant cultures of bovine trachea. 19744604399
mycoplasma viruses: isolation, physicochemical, and biological properties. 19744604802
aspiculamycin, a new cytosine nucleoside antibiotic. iv. antimycoplasma activity of aspiculamycin in vitro and in vivo. 19744616935
proceedings: fluorescent labelling with 5-dimethyl-aminonaphthalene-1-sulfonyl chloride of membrane surface proteins of gram-negative bacteria. 19744618247
radiobiological properties of acholeplasma laidlawii b. 19744822050
replacement of the octadecenoic acid growth-requirement for acholeplasma laidlawii a by cis-9,10-methylenehexadecanoic acid, a cyclopropane fatty acid. 19744820352
cholesterol in mycoplasma membranes. i. kinetics and equilibrium studies of cholesterol uptake by the cell membrane of acholeplasma laidlawii. 19744841672
a simplified method for the detection of mycoplasma. 19744858125
regulation of membrane lipid fluidity in acholeplasma laidlawii: effect of carotenoid pigment content. 19744366832
[isolation of membrane preparations from acholeplasma laidlawii cells and their transport characteristics]. 19744603647
control of membrane lipid fluidity in acholeplasma laidlawii. 19744368536
lipid and temperature dependence of membrane-bound atpase activity of acholeplasma laidlawii. 19744371925
differential scanning calorimetry. 19744614000
differential effect of phenethyl alcohol on mycoplasmas and enveloped viruses.mycoplasmas are totally inactivated by a 20-min treatment with 1% phenethyl alcohol, whereas suspensions of enveloped viruses resist the same treatment. this treatment has proved successful in eliminating mycoplasma contamination of a virus suspension.19744372415
requirements of acholeplasma laidlawii a, strain la 1, for nucleic acid precursors. 19744471597
a whole cell mounting technique for detecting acholeplasma laidlawii a on the surface of cultured cells. 19744620358
[maltose utilization by acholeplasma laidlawii cells]. 19744407457
correlation of cholesterol to phospholipid content in membranes of growing mycoplasmas. 19744426401
acholeplasma laidlawii and pasteurella multocida isolated from the pneumonic lung of a lynx. 19744436927
radioprotection of acholeplasma laidlawii b by cysteine. 19744545833
polyene antibiotic-sterol interactions in membranes of acholeplasma laidlawii cells and lecithin liposomes. i. specificity of the membrane permeability changes induced by the polyene antibiotics. 19744546884
[sensitivity range of gram-negative microorganisms and mycoplasms to minocycline in vitro]. 19744373015
induction of interferon in mice by mycoplasmas.interferon was induced in mice after intraperitoneal inoculation with four different mycoplasmas. peak levels of between 100 and 300 u of interferon per ml were attained by 6 h postinfection with each of the mycoplasmas except mycoplasma arthritidis, which induced higher titers (400 to 11,800 u/ml) by this time. a fifth mycoplasma, m. pulmonis, induced interferon inconsistently and at a later (72 to 96 h) time. mycoplasmatales virus mvl51 and sterile mycoplasmal broth did not stimulate interfero ...19744373395
cholesterol in mycoplasma membranes. ii. components of acholeplasma laidlawii cell membranes responsible for cholesterol binding. 19744378339
isolation, solubilization, and reconstitution of mycoplasma membranes. 19744378340
localization of the tween 20-soluble membrane proteins of acholeplasma laidlawii by crossed immunoelectrophoresis. 19744137837
[mixed infection of mice of line bal/b/cde with rauscher virus and mycoplasma laidlawii]. 19744446553
the effect of membrane-lipid phase transitions on membrane structure and on the growth of acholeplasma laidlawii b. 19744461849
reconstitution of mycoplasma membranes. 19744461850
use of the avidin-biotin complex for specific staining of biological membranes in electron microscopy.to expand the electron microscopist's options in localization and visualization, a new and general staining technique has been tested. the avidin-biotin complex serves as a coupling between the electron-dense marker, ferritin, and points of interest in biological samples. when specific cellular components are tagged with biotin, those components may be visualized with ferritin-linked avidin. because of the remarkably strong affinity of avidin and biotin (characterized by an association constant ...19744139715
characterization of the mycoplasma membrane proteins. iv. disposition of proteins in the membrane. 19744407052
aminopeptidase activity of ureaplasma urealyticum. 19744533227
protection of acholeplasma laidlawii b by superoxide dismutase. 19744548416
studies with lectins on the surface carbohydrate structures of mycoplasma membranes.the surface carbohydrate structures on the cell membranes of various mycoplasma species have been investigated by using lectins, which are sugar-specific proteins. carbohydrate structures presumably bound to glycolipids, with both galactose and glucose units, were found to be exposed on the surface of mycoplasma pneumoniae and its temperature-sensitive mutants, m. mycoides var. mycoides and capri, m. pulmonis, m. gallinarum, and m. gallisepticum. lipid-bound glucose was found on m. neurolyticum. ...19744138526
an electron-microscopic examination of certain bovine mycoplasmas stained with ruthenium red and the demonstration of a capsule on mycoplasma dispar. 19744139238
incidence and significance of mycoplasmas in sick cats. 19744206697
immunological analysis of glycolipids and lipopolysaccharides derived from various mycoplasmas.the lipids and lipopolysaccharides of five mycoplasmas were examined for complement-fixing activity to antimembrane rabbit sera. total glycolipid fractions and the aqueous phenol fractions (lipopolysaccharides) from the membranes of acholeplasma laidlawii, a. modicum, a. axanthum, and mycoplasma neurolyticum exhibited significant antigenic activity. the glycolipids and phosphoglycolipids from thermoplasma acidophilum were either anticomplementary or did not react due to their extreme hydrophobic ...19744215761
development of high resolution pyrolysis-gas chromatography for the identification of microorganisms. 19744217802
polyene antibiotic-sterol interactions in membranes of acholesplasma laidlawii cells and lecithin liposomes. ii. temperature dependence of the polyene antibiotic-sterol complex formation. 19744277610
spiroplasmas are the causal agents of citrus little-leaf disease.a spiroplasma isolated from citrus with little-leaf disease was grown in a cell-free medium and injected into leafhoppers (euscelis plebejus). injected leafhoppers, but not those fed on infected plants, transmitted the spiroplasma to white clover (trifolium repens cv. s100) and sweet orange (citrus sinensis cv. valencia). infected clover plants were severely stunted; infected sweet orange plants showed typical symptoms of citrus little-leaf disease. the spiroplasma was detected in clover and swe ...197419280788
mycoplasma infection of geese. 1. incidence of mycoplasmas and acholeplasmas in geese.this paper presents data about the isolation of members of the order mycoplasmatales from material of goose origin. acholeplasma laidlawii strains were isolated from 2 to 8 day old goslings with heavy fibrinous airsacculitis, peritonitis and perihepatitis. losses reached 30% of the flock by the end of the 8th week of age. acholeplasma axanthum strains were detected in goose-embryos that died on the 13th day of incubation. a significant loss (up to 60%) of embryos was observed in the flock and so ...197518777290
transport properties of membrane vesicles from acholeplasma laidlawii. ii. kinetic characteristics and specificity of glucose transport system.the glucose transport system of membrane vesicles isolated from acholeplasma laidlawii is saturable, with a km of 21.2 mum and v of 0.68 nmol min-1 (mg protein)-1. the process is ph-dependent and a break occurs in the arrhenius plot at 15 degrees c. exogenous substrates did not stimulate glucose transport probably due to their inability to penetrate into membrane vesicles. 3-o-methylglucose and 6-deoxyglucose competitively inhibited glucose transport. maltose inhibited transport of glucose nonco ...1975286
transport properties of membrane vesicles from acholeplasma laidlawii. i. isolation and general characteristics.a new procedure for the isolation of membrane vesicles from acholeplasma laidlawii cells is described. the membrane vesicles are completely free from contaminations of whole cells and cell debris and represent a homogeneous fraction as shown by electron microscopy, ficoll density-gradient centrifugation, and titration on agar plate. absence of cytoplasmic contaminations was confirmed by double-labelling of membranes with 3h-oleic acid and 14c-uridine, as well as by distribution of specific marke ...1975127739
differential fluorescence labelling with 5-dimethyl-aminonaphthalene-1-sulfonyl chloride of intact cells and isolated membranes in salmonella typhimurium and acholeplasma laidlawii.for fluorescence labelling intact cells and isolated cell envelopes (membranes) from salmonella typhimurium and acholeplasma laidlawii were treated with mixed dansylchloride-lecithin-cholesterol vesicles. this kind of dansylation, which has been supposed to be specific for cell surface proteins, produced fluorescent protein pattern after sds-polyacrylamide gel electrophoresis only when isolated envelopes were dansylated. acid hydrolysis of fluorescent cell envelopes of salmonella typhimurium yie ...1975235245
[glucose transport into membrane vesicles from acholeplasma laidlawii cells]. 1975235409
electron diffraction studies of biological membranes and lipids. 1975235744
[identification of mycoplasmas isolated from monkeys].the authors identified 15 strains of mycoplasmae isolated from papio hamadryas suffering from leukemia and from healthy macacus rhesus, a green monkey and saimiri. a study was made of their biochemical properties, antigenic properties in the reaction of growth depression with immune sera to a number of standard strains, and also the protein composition by electrophoresis in polyacrylamide gel (epag). a number of mycoplasmae were identified as a. laidlawii, m. arthritidis--campo and m. cynomolgus ...1975235821
adenine formation from adenosine by mycoplasmas: adenosine phosphorylase activity.mammalian cells have enzymes to convert adenosine to inosine by deamination and inosine to hypoxanthine by phosphorolysis, but they do not possess the enzymes necessary to form the free base, adenine, from adenosine. mycoplasmas grown in broth or in cell cultures can produce adenine from adenosine. this activity was detected in a variety of mycoplasmatales, and the enzyme was shown to be adenosine phosphorylase. adenosine formation from adenine and ribose 1-phosphate, the reverse reaction of ade ...1975236559
immunogenetic analysis of the mechanism of induction of friend virus leukemia. 1975236610
scanning of polyacrylamide gel electrophoresis columns for detection of unstained protein zones and for their localization relative to enzyme activities. 1975236684
crossed immunoelectrophoresis: polyacrylamide gel electrophoresis followed by electrophoresis into an agarose gel containing antibodies. 1975236696
osmotic behaviour of acholeplasma laidlawii b cells with membrane lipids in liquid-crystalline and gel state.the osmotic behaviour of acholeplasma laidlawii b cells was investigated with combined spectrophotometric and enzymatic measurements. the conclusion could be drawn that this osmotic behaviour depends largely on the physical state of the membrane lipids. when part of the membrane lipids is in the liquid-crystalline phase the cell is able to swell and behaves as a good osmometer. however, when the membrane lipid is in the gel phase, the cell is unable to swell and the change in absorbance of the c ...1975236790
glucose transport in acholeplasma laidlawii b: dependence on the fluidity and physical state of membrane lipids.the uptake of d-glucose by acholeplasma laidlawii b occurs via a mediated transport process, as shown by the following observations: (i) glucose permeates a. laidlawii b cells at a rate at least 100 times greater than would be expected if its entry occurred only by simple passive diffusion; (ii) the apparent activation energy for glucose uptake in a. laidlawii is significantly lower than that expected and observed for the passive permeation of this sugar; (iii) glucose uptake appears to be a sat ...1975237881
heterogeneity in the physical state of the exterior and interior regions of mycoplasma membrane lipids. 1975238513
membrane lipid, not polarized water, is responsible for the semipermeable properties of living cells.it has recently been proposed that the semipermeable surface barrier of living cells is in fact provided by water polarized in multilayers by the cellular protein, and not by the lipids of the cell membrane as had been widely supposed. this review paper summarizes a large and diverse body of recent experimental work, concerned with the structure and function of biological membranes in general and with the passive permeability properties of cell surface membranes in particular, which does not sup ...1975238671
electron-microscopic study of a mycoplasmatales virus, strain mv-lg-ps2-l 172.morphology and adsorption of a globular virus, lysing acholeplasma laidlawii were studied in ultrathin sections of plaques in a lawn of the host strain. the virus was globular, about 50 to 90 nm in diameter, with a clearly defined membrane, 6.5 to 8 nm thick. a protuberance about 25 to 35 nm long and 12 to 20 nm thick was observed on numerous virus particles. the evenly granulated, electron-optically dense content of the cells became clearer in cells affected by the viruses. fibrillar structures ...1975234904
purification of membrane proteins from acholeplasma laidlawii by agarose suspension electrophoresis in tween 20 and polyacrylamide and dextran gel electrophoresis in detergent-free media.four of the membrane proteins from acholeplasma laidlawii that are soluble in the nonionic detergent tween 20 have been purified by preparative electrophoretic techniques utilizing different supporting media. the last purification step for two of the major proteins was a preparative polyacrylamide gel electrophoresis performed in the absence of any detergent. the proteins were recovered by continuous elution. the purity of the fractions was examined by analytical polyacrylamide gel electrophores ...1975235514
[infectious keratoconjunctivitis of cattle in ivory coast. i. microbiological aspects]. 1975174165
characterization of the mycoplasma membrane proteins. v. release and localization of membrane-bound enzymes in acholeplasma laidlawii.the peripheral membrane protein fraction released by washing acholeplasma laidlawii membranes with low-ionic strength buffers contained about 50% of the total membrane-bound ribonuclease and deoxyribonuclease activities. the atpase, nadh oxidase and p-nitrophenylphosphatase activities remained bound to the membrane even when edta was added to the wash fluids, and thus appear to belong to the integral membrane protein group. serving as a marker for peripheral membrane proteins, the membrane-bound ...1975234252
cholesterol incorporation into bacterial membranes.the wall-covered bacteria micrococcus lysodeikticus, bacillus megaterium, and proteus mirabilis incorporated exogenous cholesterol into their cytoplasmic membrane in quantities resembling those incorporated by sterol-nonrequiring mycoplasmas. cholesterol incorporation into the outer membrane of p. mirabilis was much more restricted than into the cytoplasmic membrane.1975809427
lipid-lipid and lipid-protein interaction in model systems and membranes. 1975769638
recent progress in mycoplasma research.today, 12 years after the identification of eaton agent as a mycoplasma, much new information on this infection has accumulated. better methods have become available, which will be helpful in determining whether other mycoplasmas might be human pathogens, e.g., some ureaplasmas in nongonococcal urethritis. experimental mycoplasma infections in birds and animals have revealed common features with immune complex diseases in man. they may serve, therefore, as useful models for human mycoplasma dise ...19751081171
survival of frozen mycoplasmas.cooling to -70 c killed a higher percentage of acholeplasma laidlawii and mycoplasma mycoides var. capri cells than cooling to -20 c. however, to preserve cell viability for prolonged periods storage at -70 c was much more preferable. the percentage of cells surviving freezing could be increased by increasing the initial cell concentration or by the addition of dimethyl sulfoxide or glycerol as cryoprotective agents. in the presence of 1.5 m of any one of these agents survival rates of up to 100 ...19751099985
[respiratory activity of acholeplasma laidlawii cells and its role in the active transport of carbohydrates].the respiratory activity of the acholeplasma laidlawii cells was studied in order to elucidate a possible mechanism of coupling of transport with energy. the respiration of the cells is stimulated by ethanol, glucose, nadh, lactate, and pyruvate. the substrates of the krebs cycle have no effect on the respiration. the respiratory activity, stimulated by ethanol and glucose, is inhibited by the inhibitors of the respiratory chain, sh reagents, and the inhibitors of glycolysis. the results of expe ...19751207494
immunological properties of glycolipids from membranes of acholeplasma laidlawii.glycolipids, the predominant class of lipids in the membranes of acholeplasma laidlawii, are the haptenic determinants that react with anti-a. laidlawii serum to fix complement. the predominant complement-fixing activity of the membrane glycolipids was associated with the monoglucoysyl diglyceride, diglucosyl diglyceride, glycerlphosphoryl diglucosyl diglyceride (gpdd), and an unknown lipid b, which did not react with ninhydrin but release glucose and glycerol and traces of phosphorus upon hydro ...19751193716
mycoplasmas and cuffing pneumonia in a group of calves.the mycoplasmas found in the lungs of 20 calves, housed together for six months, and the related pulmonary pathology are reported. twelve calves had cuffing pneumonia and in this group there was a significantly higher isolation frequency of mycoplasma dispar and ureaplasma spp compared with the non-pneumonic group. mycoplasma bovirhinis and acholeplasma laidlawii were isolated from the lungs of calves in both groups. mycoplasma arginini was not recovered from the lungs of any calf. the significa ...19751198879
lipid-sterol interactions in liposomes and membranes. 19751193252
drying and irradiation of calf and horse serum. ii. influence on mycoplasma content of the calf sera.drying of calf sera produced only a negligible change in their content of acholeplasma laidlawii organisms and reduced the content of mycoplasma arginini organisms by 2 to 4 logs. gamma-irradiation of liquid and dried calf sera killed m. arginini and a. laidlawii organisms at irradiation levels of 0.2-0.3 and 0.4-0.6 mrads, respectively.19751179873
replication of mycoplasmavirus mvl51: i. replicative intermediates. 19751180927
[radiosensitivity of mycoplasma and damage to their dna after irradiation (author's transl)]. 19751242851
molecular order in acholeplasma laidlawii membranes as determined by deuterium magnetic resonance of biosynthetically-incorporated specifically-labelled lipids.the first application of deuterium magentic resonance of specifically labelled lipids to the study of a natural biological membrane is described. palmitic acid labelled at the terminal methyl group with deuterium was incorporated biosynthetically into the lipids of the plasma membrane of acholeplasma laidlawii. the deuterium nuclear magnetic resonance spectra contain quadrupole splittings which yield directly order parameters for this region of the membrane. below the growth temperature (37 degr ...19751182151
characterization of mycoplasmatales virus-laidlawii 3.purified preparations of mycoplasmatales virus-laidlawii 3 were negatively stained and studied by electron microscopy. they were seen to consist of uniform sized particles having a polyhedral head, 57 nm by 61 nm, and a short tail, 25 nm long, joined to the head at one vertex by a collar. the particles were shown to have buoyant densities of 1-477 g/ml in csc1, 1-32 g/ml and 1-26 g/ml in potassium tartrate and a sedimentation coefficient, s20,w, of 290 +/- 13. they are composed of 35-2% double-s ...19751185149
phase transitions and fluidity characteristics of lipids and cell membranes. 19751103214
mycoplasma species recovered from the reproductive tracts of western canadian cows.samples of cervico-vaginal mucus from 633 animals from 110 herds were cultured and yielded the following mycoplasmas: t-strain--88: mycoplasma bovigenitalium--79, mycoplasma spp. (leach group 7)--7, acholeplasma laidlawii--4, mycoplasma bovirhinis--2 and one not typable. uterine exudates and endometrial scrapings from 80 infertile cows in two herds were examined. four animals were positive, m. bovigenitalium was isolated three times, a. laidlawii and mycoplasma arginini once each. sixty-five nor ...19751125831
transport properties of membrane vesicles from acholeplasma laidlawii. iii. evidence of active nature of glucose transport.membrane vesicles obtained from acholeplasma laidlawii accumulate glucose as well as maltose and fructose against their concentration gradient in the absence of exogenous energy sources. glucose uptake by membrane vesicles is inhibited by anaerobiosis and by electron transfer inhibitors, such as rotenone and amytal, but not by 2-heptyl-4-hydroxyquinoline n-oxide, antimycin a, cyanide and azide. rotenone, cyanide and amytal also produce a rapid efflux of glucose from the membrane vesicles. arsena ...19751193497
serum lipoproteins as cholesterol donors to mycoplasma membranes. 19761252244
experimentally produced calf pneumonia.experimental pneumonia was produced in calves by the endobronchial inoculation of pneumonic lung homogenates. irradiated homogenates produced minimal pneumonia. ampicillin treatment of the homogenates and the experimental calves reduced the extent of pneumonia. treatment with tylosin tartrate prevented experimental pneumonia. these results suggest that the total pneumonia was due to organisms susceptible to tylosin tartrate and that the residual pneumonia remaining after ampicillin treatment was ...19761265354
infectious bovine keratoconjunctivitis: experimental induction of infection in calves with mycoplasmas and moraxella bovis.eyes of 14 calves were exposed by conjunctival instillation to cultures of either mycoplasma conjunctivae (6 calves) or acholeplasma laidlawii (8 calves). calves were observed for clinical signs of infectious bovine keratoconjunctivitis (ibk), and eyes were examined for the test organisms by bacteriologic cultural technique for 60 days. acholeplasma laidlawii became established in the eyes of 5 of 8 calves; m conjunctivae became established in the eyes of 4 of 6 calves. on day 28, eyes of 9 of t ...19761275332
[localization and possible development cycle of mycoplasmas and bacterial l-forms in cell cultures].using vital stain chlorazol-black e the authors studied the localization and behaviour of 5 mycoplasma species and the stable l-form of beta-hemolytic streptococcus of group a in various continuous cell lines. mycoplasmas and l-forms had a definite evolution cycle in the cells. at first they are arranged extracellularly and on the surface of the cells, and then intracellularly where they multiply intensively; later they are again localized on the surface of the cells and extracellularly. this cy ...19761276447
acholeplasma laidawii mutant defective in glucose transport system. 19761001438
spread and control of mycoplasmal infection of cell cultures.environmental sampling was performed during trypsinization and passage of 3t-6 cell cultures that contained a mean of 4.3 x 10(7) colony forming units (cfu) per ml supernatant of a. laidlawii. the lip of the culture flask and the outside of the used pipet were always heavily contaminated. the outside of the culture flask (3/7), the work surface (8/12) and the outside of a pan of disinfectant (4/5) were regularly contaminated with mycoplasmas. airborne mycoplasmas were detected eight of 32 times ...19761034618
isoelectric focusing of mycoplasma proteins.polyacrylamide gel isoelectric focusing (pagif) in thin layer was used to resolve proteins of mycoplasma spp., acholeplasma spp., and eight strains of ureaplasma urealyticum (t-strain). a mixture of urea, triton x-100, and dithioerythritol was used to solubilize sonically disrupted cells. pagif was performed in the range of ph 3 to 10. protein patterns were carefully compared, demonstrating resolved and distinguishable species-specific protein bands. the eight serotypes of u. urealyticum (t-stra ...1976791125
effect of cerulenin on growth and lipid metabolism of mycoplasmas.cerulenin markedly inhibited the growth of acholeplasma laidlawii. a. axanthum and a. granularum were less susceptible, whereas the sterol-requiring mycoplasma species examined showed very little susceptibility. the inhibition was not reversed by the addition of long-chain fatty acids to the medium. at a concentration of 20 mug/ml, cerulenin inhibited the incorporation of [(14)c]acetate into a. laidlawii membrane lipids, but it had no effect on either protein or nucleic acid biosynthesis. cerule ...19761267428
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