| characterization of the myoplasma membrane proteins. 3. gel filtration and immunological characterization of acholeplasma laidlawii membrane proteins. | | 1972 | 4339322 |
| elimination of mycoplasma contaminants from virus stocks by treatment with nonionic detergents. | five nonionic detergents (tweens 20, 40, 60, and 80, and triton wr-1339) were tested for their ability to inactivate four mycoplasma species which are common contaminants of animal cell cultures. tween 20 was found to be the most effective, in that a concentration of 2.5 mg/ml completely inactivated cultures of m. hominis, m. hyorhinis, and acholeplasma laidlawii within 1 hr and a culture of m. orale within 3 hr. the other detergents exhibited various degree of activity against the different myc ... | 1972 | 4341521 |
| [immunodepressive effect of mycoplasma laidlawii on the formation of humoral antibodies in mice]. | | 1972 | 4344075 |
| the structure of a glycerylphosphoryldiglucosyl diglyceride from the lipids of acholeplasma laidlawii strain b. | 1. the phosphatidylglucose structure proposed previously (smith & henrikson, 1965) for the glucose-containing phospholipid from acholeplasma laidlawii is incorrect. 2. the structure now proposed is 3-(sn-glycerol-3-phosphoryl-6'-[o-alpha-d-glucopyranosyl-(1-->2)-o-alpha-d-glucopyranosyl])- sn-1,2-diglyceride, a new type of bacterial lipid. 3. deacylation of the lipid gave a single water-soluble phosphate ester which could be distinguished on chromatography from synthetic samples of glucosylphosp ... | 1972 | 4345854 |
| plasticity of biological membranes. | | 1972 | 4346307 |
| membrane lipids of mycoplasma hominis. | essentially all of the lipids of mycoplasma hominis (200 mug/mg of cell protein) were found to be located in the cell membrane. over one-half were neutral lipids incorporated from the growth medium and consisting of 43% free cholesterol, 19% esterified cholesterol, 23% triglycerides, 10% free fatty acids, and small amounts of di- and monoglycerides. the polar lipids accounting for about 40% of the total were synthesized by the organisms. phosphatidylglycerol was the predominant lipid of this fra ... | 1973 | 4347923 |
| isolation and characterization of mycoplasma conjunctivae sp. n. from sheep and goats with keratoconjunctivitis. | fourteen conjunctival strains of mycoplasma were isolated from infected sheep and goats in two separate naturally occurring outbreaks of keratoconjunctivitis (pink-eye). the biological and serological properties and cell protein patterns of these strains are presented in this report. these strains were related to each other and were unrelated to 40 recognized mycoplasma and acholeplasma species and serotypes. we are proposing that this group of mycoplasmas be named m. conjunctivae. the role of m ... | 1972 | 4348078 |
| virus-like particles associated with the mycoplasmas of clover phyllody in the plant and in the insect vector. | | 1973 | 4348972 |
| dilatometry biological membranes. | | 1973 | 4350105 |
| effect of proteins on the motion of spin-labeled fatty acids in mycoplasma membranes. | | 1973 | 4350558 |
| the effect of different fatty acid and sterol composition on the erythritol flux through the cell membrane of acholeplasma laidlawii. | | 1973 | 4352491 |
| binding of proteins to mycoplasma membranes. | | 1973 | 4354127 |
| reaction of mice on infection with mycoplasma and leukosis viruses. | | 1973 | 4354218 |
| mycoplasma-associated induction of interferon in ovine leukocytes. | a mycoplasmal species, acholeplasma laidlawii, isolated as a contaminant from a fetal lamb kidney cell line, was shown to be associated with the induction of interferon in cultures of ovine peripheral blood leukocytes. broth cultures of the mycoplasma induced between 20 and 230 u of interferon per ml in leukocytes from two adult ewes. the amount of interferon produced correlated with the inoculum size of mycoplasma. interferon production was associated with replication of the mycoplasma in the l ... | 1973 | 4356073 |
| lipid- and temperature-dependent structural changes in acholeplasma laidlawii cell membranes. | | 1973 | 4357019 |
| phosphatase activity of some mycoplasma species. | | 1971 | 4361836 |
| regulation of membrane lipid fluidity in acholeplasma laidlawii: effect of carotenoid pigment content. | | 1974 | 4366832 |
| control of membrane lipid fluidity in acholeplasma laidlawii. | | 1974 | 4368536 |
| lipid and temperature dependence of membrane-bound atpase activity of acholeplasma laidlawii. | | 1974 | 4371925 |
| differential effect of phenethyl alcohol on mycoplasmas and enveloped viruses. | mycoplasmas are totally inactivated by a 20-min treatment with 1% phenethyl alcohol, whereas suspensions of enveloped viruses resist the same treatment. this treatment has proved successful in eliminating mycoplasma contamination of a virus suspension. | 1974 | 4372415 |
| [sensitivity range of gram-negative microorganisms and mycoplasms to minocycline in vitro]. | | 1974 | 4373015 |
| induction of interferon in mice by mycoplasmas. | interferon was induced in mice after intraperitoneal inoculation with four different mycoplasmas. peak levels of between 100 and 300 u of interferon per ml were attained by 6 h postinfection with each of the mycoplasmas except mycoplasma arthritidis, which induced higher titers (400 to 11,800 u/ml) by this time. a fifth mycoplasma, m. pulmonis, induced interferon inconsistently and at a later (72 to 96 h) time. mycoplasmatales virus mvl51 and sterile mycoplasmal broth did not stimulate interfero ... | 1974 | 4373395 |
| cholesterol in mycoplasma membranes. ii. components of acholeplasma laidlawii cell membranes responsible for cholesterol binding. | | 1974 | 4378339 |
| isolation, solubilization, and reconstitution of mycoplasma membranes. | | 1974 | 4378340 |
| characterization of the mycoplasma membrane proteins. iv. disposition of proteins in the membrane. | | 1974 | 4407052 |
| [maltose utilization by acholeplasma laidlawii cells]. | | 1974 | 4407457 |
| correlation of cholesterol to phospholipid content in membranes of growing mycoplasmas. | | 1974 | 4426401 |
| acholeplasma laidlawii and pasteurella multocida isolated from the pneumonic lung of a lynx. | | 1974 | 4436927 |
| [mixed infection of mice of line bal/b/cde with rauscher virus and mycoplasma laidlawii]. | | 1974 | 4446553 |
| occurance of acholeplasma axanthum in cattle. | | 1974 | 4460932 |
| the effect of membrane-lipid phase transitions on membrane structure and on the growth of acholeplasma laidlawii b. | | 1974 | 4461849 |
| reconstitution of mycoplasma membranes. | | 1974 | 4461850 |
| [interferon and antiviral activity induced "in vivo" by a plant mycoplasma, "acholeplasma" species phig1 (author's transl)]. | | 1974 | 4467769 |
| requirements of acholeplasma laidlawii a, strain la 1, for nucleic acid precursors. | | 1974 | 4471597 |
| rapid detection of bovine mycoplasma antigens by counterimmunoelectrophoresis. | twelve reference strains of mycoplasma and acholeplasma previously reported to have been recovered from cattle were tested against hyperimmune rabbit serum by counterimmunoelectrophoresis. this technique detected antigen by the formation of precipitin lines with antibody within 1 h and promises to be a useful technique for detecting and identifying mycoplasma isolates in either pure or mixed cultures. | 1974 | 4474834 |
| the growth and morphology of acholeplasma (mycoplasma) laidlawii a in different media. | | 1972 | 4505769 |
| transfection mediated by mycoplasmatales viral dna. | dna isolated from mycoplasmatales viruses mvl51 and mvgs51 was infectious when mixed with acholeplasma laidlawii bn1-na1(r) cells. infectivity was destroyed by deoxyribonuclease but not by ribonuclease, pronase, or specific antiserum to the virus. host mycoplasma cells were only competent for transfection during late-log growth phase. the rates of the establishment of dnase insensitivity of viral dna transfectants were similar to those of bacteriophage systems. the dose-response curve for transf ... | 1972 | 4508332 |
| nmr studies of sodium and potassium in various biological tissues. | | 1973 | 4513156 |
| fusion of phospholipid vesicles with viable acholeplasma laidlawii. | when vesicles of dipalmitoyl phosphatidylcholine produced by sonication are mixed with acholeplasma laidlawii in neutral buffer, a phenomenon occurs that is most readily explained in terms of fusion of lipid vesicle membrane with the mycoplasma cytoplasmic membrane. the mycoplasma can readily accumulate large quantities of the foreign lipid without loss of viability. the added lipids do not remain in patches but diffuse laterally throughout the mycoplasma membrane. | 1973 | 4515622 |
| studies on the pathogenicity of acholeplasma axanthum in swine. | acholeplasma axanthum sp. was isolated from the lung of swine with catarrhal pneumonia. clinical symptoms of respiratory disease, gross and histological lesions of pneumonia, as well as serological response were produced by intranasal inoculation of ;miniature pigs' with the supernatant of lung suspension containing acholeplasma axanthum and by a 48 hr. broth culture of the strain.a similar picture of disease was observed in animals held in contact with the animals inoculated with untreated lung ... | 1974 | 4522723 |
| aminopeptidase activity of ureaplasma urealyticum. | | 1974 | 4533227 |
| radioprotection of acholeplasma laidlawii b by cysteine. | | 1974 | 4545833 |
| polyene antibiotic-sterol interactions in membranes of acholeplasma laidlawii cells and lecithin liposomes. i. specificity of the membrane permeability changes induced by the polyene antibiotics. | | 1974 | 4546884 |
| polyene antibiotic-sterol interactions in membranes of acholeplasma laidlawii cells and lecithin liposomes. 3. molecular structure of the polyene antibiotic-cholesterol complexes. | | 1974 | 4546885 |
| protection of acholeplasma laidlawii b by superoxide dismutase. | | 1974 | 4548416 |
| the effect of aeration on the growth of acholeplasma (mycoplasma) laidlawii a. | | 1972 | 4553303 |
| [comparative characteristics of methods of culturing mycoplasma and l-forms of bacteria and serological reactions used for their identification]. | | 1972 | 4555023 |
| osmolar concentration and fixation of mycoplasmas. | broth cultures of acholeplasma laidlawii were fixed with various concentrations of cacodylate-buffered glutaraldehyde. the shape and ultrastructure of the organisms varied with the osmolar concentration of the fixative. when the fixation mixture was hypertonic to the culture medium, ultrathin sections suggested that the cells had shrunk. phosphate buffer, sodium chloride, or sucrose at comparable osmolaities had the same effect as sodium cacodylate. glutaraldehyde itself also contributed to the ... | 1972 | 4555408 |
| permeability and topography of membranes. | | 1972 | 4557530 |
| [studies of the biosynthesis of transfer ribonucleic acids]. | | 1972 | 4560651 |
| [mycoplasma infection of cattle]. | | 1972 | 4562052 |
| genome size and evolution. | | 1973 | 4565043 |
| studies on the biological properties of polyene antibiotics: comparison of other polyenes with filipin in their ability to interact specifically with sterol. | | 1972 | 4565643 |
| workshop on the mycoplasmatales as agents of disease. session i. biology of the mycoplasmatales. summary of discussion. | | 1973 | 4570125 |
| acyl carrier protein in mycoplasmas. | acyl carrier protein (acp) activity was determined by the malonyl-coenzyme a-co(2) exchange reaction. it was highest in acholeplasma laidlawii, lower in a. granularum, and lowest in a. axanthum. the sterol-requiring mycoplasma species examined showed little or negligible acp activity. a. laidlawii was capable of utilizing pantetheine or coenzyme a but not beta-alanine as precursor for acp synthesis. its acp could thus be labeled by growing the organisms with radioactive coenzyme a. the acp of a. ... | 1973 | 4570599 |
| eagle's basal medium as a defined medium for mycoplasma studies. | | 1972 | 4571002 |
| characterization of mycoplasmatales virus dna. | | 1973 | 4573075 |
| are cell membranes fluid? | | 1973 | 4574849 |
| further studies on classification of bovine strains of mycoplasmatales, with proposals for new species, acholeplasma modicum and mycoplasma alkalescens. | | 1973 | 4578968 |
| recent studies using nuclear magnetic resonance spectroscopy of lipids and biological membranes. | | 1972 | 4580642 |
| characterization of the membranes of thermoplasma acidophilum. | thermoplasma acidophilum grows optimally under aeration at 59 c and ph 2. both intact cells and membranes disaggregate below ph 1 and above ph 5, producing no sedimentable particles. increase in ionic strength at ph 5 or below results in cellular lysis and membrane disaggregation. membranous components produced by lysis at alkaline ph reaggregate upon reduction of both ph and ionic strength. osmotic environment plays little role in cellular stability. membranes prepared by sonic lysis at ph 5 ex ... | 1973 | 4583228 |
| cultivation of mycoplasmas on cellulose ester substrates. | the ability of mycoplasmas to grow on cellulose ester substrates was evaluated. mycoplasma pneumoniae, m. hominis, m. arthritidis, m. gallisepticum, and acholeplasma laidlawii grew on millipore (mixed cellulose ester) filters and sepraphore iii (cellulose polyacetate) membranes. | 1973 | 4584581 |
| selective membrane toxicity of the polyene antibiotics: studies on natural membranes. | the effect of polyene antibiotics on candida albicans, human erythrocytes, and acholeplasma laidlawii was studied. the results sustain the observations made with lecithin-sterol liposomes. the distribution of double bonds in the membrane sterol nucleus appears to be of major importance in conferring polyene susceptibility; those sterols with the ergosterol nucleus are far more effective than those with a nucleus similar to cholesterol. different polyenes vary in their membrane selectivity. the c ... | 1973 | 4586145 |
| protein-lipid interactions. | | 1972 | 4592575 |
| study of acholeplasma strains isolated from swine. | | 1973 | 4595933 |
| isolation of acholeplasma axanthum from swine. | | 1973 | 4595934 |
| [identification of mycoplasma and bacterial l forms by polyacrylamide gel electrophoresis]. | | 1974 | 4598211 |
| isolation of acholeplasma laidlawii from commercial, serum-free tissue culture medium and studies on its survival and detection. | this report documents the first isolation of acholeplasma laidlawii from a commercial lot of serum-free dulbecco basal medium. experimental studies demonstrated survival of the organism for at least 1 year depending on the concentration of the contaminating organism as well as ph and temperature of storage of the serum-free medium. a comparison of isolation methods showed that concentration by filtration through 220-nm membrane filters and testing the filters for mycoplasma recovery, especially ... | 1974 | 4598432 |
| induction of interferon in ovine leukocytes by species of mycoplasma and acholeplasma. | | 1974 | 4599490 |
| epidemiologic observations on mycoplasma mastitis. | | 1974 | 4602678 |
| [isolation of membrane preparations from acholeplasma laidlawii cells and their transport characteristics]. | | 1974 | 4603647 |
| effect of mycoplasma dispar, mycoplasma bovirhinis, acholeplasma laidlawii and t-mycoplasmas on explant cultures of bovine trachea. | | 1974 | 4604399 |
| mycoplasma viruses: isolation, physicochemical, and biological properties. | | 1974 | 4604802 |
| differential scanning calorimetry. | | 1974 | 4614000 |
| aspiculamycin, a new cytosine nucleoside antibiotic. iv. antimycoplasma activity of aspiculamycin in vitro and in vivo. | | 1974 | 4616935 |
| proceedings: fluorescent labelling with 5-dimethyl-aminonaphthalene-1-sulfonyl chloride of membrane surface proteins of gram-negative bacteria. | | 1974 | 4618247 |
| a whole cell mounting technique for detecting acholeplasma laidlawii a on the surface of cultured cells. | | 1974 | 4620358 |
| proton magnetic resonance studies of whole human erythrocyte membranes. | | 1972 | 4622201 |
| mycoplasma: growth precipitation as a serodiagnostic method. | a serological test is described which is designated the growth precipitation test and is based on the release of diffusible compounds from mycoplasma strains. during growth of mycoplasmas on solid substrates, precipitation lines are formed with antibodies from homologous antisera independent of heat-labile serum factors. the application of the test as a serodiagnostic tool was investigated by performing a complete serological cross-test with 10 mycoplasma strains. the test was compared with the ... | 1972 | 4623280 |
| detection of cholesterol in cell membranes by use of bacterial toxins. | a method is described for the detection of cholesterol in membranes from erythrocytes, mycoplasmas, and bacterial cells by a ferritin-labeling technique. membranes treated with cereolysin, a bacterial hemolysin which specifically binds to cholesterol, and then treated with ferritin-antitetanolysin, were specifically ferritin-labeled for cholesterol. a similar antigen-antibody system, streptolysin o-ferritin-antistreptolysin, was also used successfully with erythrocyte membranes. there was an une ... | 1972 | 4623312 |
| identification and characterization of acholeplasma oculusi spec. nov. from the eyes of goats with keratoconjunctivitis. | | 1973 | 4631442 |
| divalent cations in native and reaggregated mycoplasma membranes. | the mg(2+) content of membranes of several mycoplasma and acholeplasma species varied between 0.88 and 1.98 mug of mg(2+) per mg of protein, depending on the species and on growth conditions. ca(2+) could be detected only when it was added to the growth medium. the mg(2+) content of isolated a. laidlawii membranes could be increased almost threefold by dialysis against 20 mm mg(2+), whereas aggregated a. laidlawii membranes contained about six to eight times more mg(2+) per mg of protein than th ... | 1973 | 4632319 |
| [active glucose transport in mycoplasma laidlawii]. | | 1972 | 4636164 |
| hydrolysis of alanine oligopeptides by an enzyme located in the membrane of mycoplasma laidlawii. | | 1972 | 4640765 |
| photodynamic killing of acholeplasma laidlawii. | | 1972 | 4643094 |
| a phosphatidyl diglucosyl diglyceride from acholeplasma laidlawii b. | | 1972 | 4643340 |
| [iron oxidation by mycoplasma laidlawii]. | | 1972 | 4643941 |
| survival of acholeplasma laidlawii a and changes in its dna after irradiation with x- and gamma rays. | | 1972 | 4644259 |
| antibiotics from mycoplasma. i. acholeplasma laidlawii b. | | 1972 | 4645949 |
| [size distribution of colony-forming units in a population of mycoplasma laidlawii]. | | 1972 | 4657956 |
| [sensitivity of mycoplasmas to spiramycin]. | | 1972 | 4669219 |
| [stimulating effect of langat virus on reproduction of mycoplasma laidlawii in combined infection of a primary culture of chick embryo cells]. | | 1972 | 4672317 |
| fungistatic activity of lucerne saponins and digitonin as related to sterols. | | 1972 | 4676387 |
| relations between lipid composition and permeability of membranes. | | 1972 | 4680245 |
| freeze-fractured acholeplasma laidlawii membranes: nature of particles observed. | freeze-fracture of acholeplasma laidlawii membranes from cells incubated in the presence of puromycin or omission of amino acids reveals a decrease in the number of particles between 50 and 100 angstroms in the hydrophobic fracture plane, which strongly suggests that these particles are protein. additional evidence indicates that they may be involved in substrate transport. | 1973 | 4682132 |
| [antigenic relationship of saprophytic and plant mycoplasmas]. | | 1973 | 4683168 |
| weak association of glucosamine-containing polymer with the acholeplasma laidlawii membrane. | | 1973 | 4684605 |
| distribution of a phosphoenolypyruvate-dependent sugar phosphotransferase system in mycoplasms. | a survey of 10 mycoplasma strains has shown that their capacity to accumulate radioactivity from alpha-methyl-d-glucopyranoside depends on the activity of a phosphoenolpyruvate-dependent phosphotransferase system (pts), and that this system endows the organisms with a high affinity for glucose as a fermentation substrate. pts activity was found in mycoplasma gallisepticum, m. mycoides var. mycoides, and m. mycoides var. capri, but in none of the fermentative acholeplasma strains nor in some of t ... | 1973 | 4688137 |
| chemical composition and serology of mycoplasma pneumoniae lipids. | | 1973 | 4689627 |
| freeze-etch electron microscopy of erythrocytes, acholeplasma laidlawii cells and liposomal membranes after the action of filipin and amphotericin b. | | 1973 | 4690870 |