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[the definition and epidemiology of bacterial vaginosis].bacterial vaginosis is a condition with a flora change in the vaginal where a lactobacilli-dominated flora is exchanged with an abundant complex flora dominated by strict and facultative anaerobic bacteria. the condition seldom occurs in prepubertal girls and post-menopausal women, suggesting an hormonal component in its etiology. recurrent episodes of bacterial vaginosis are frequent. three out of four criteria should be fulfilled for establishing the diagnosis of bacterial vaginosis, i.e., an ...19938484086
[recent bacteriologic data: from physiopathology to treatment].bacterial vaginosis is characterized by a change in the vaginal ecosystem in which lactobacillus spp, the dominant members of the normal flora, are replaced by an association of various bacterial species including gardnerella vaginalis, anaerobes (bacteroides spp, prevotella spp, porphyromonas spp, peptostreptococcus spp and mobiluncus spp) and mycoplasma hominis. the reasons for this imbalance are unknown, although the loss of lactobacilli that produce hydrogen peroxide (which is toxic for g. v ...19938484089
mycoplasmalike organisms (mlos) and distinguish them from other mlos.dna of 10 lines of rice yellow dwarf (ryd) mycoplasmalike organisms (mlos) from japan, the phillippines, and thailand hybridized with four probes containing chromosomal and six probes containing extrachromosomal dna of a tochigi (japan) line of ryd mlo. one chromosomal probe (ryd9) and all six extrachromosomal probes hybridized with various other mlos (sugarcane white leaf, onion yellows, cineraria witches'-broom, japanese hornwort witches'-broom, water dropwort wiches'-broom, gentian witches'-b ...19938489230
characteristics of mycoplasma hominis adhesion.mycoplasma hominis, a human pathogen, has previously been observed to bind to sulfatide separated on thin-layer chromatograms. it has not been demonstrated, however, that the binding is not simply a nonspecific ionic interaction. the ability of a low-passage patient isolate of m. hominis to adhere to glycoconjugates other than sulfatide and the characteristics of its binding to sulfatide were studied. mycoplasmas were found to bind strongly and specifically in a temperature- and dose-dependent m ...19938491739
mycoplasma hominis in liver transplantation. 19938441972
minocycline compared with doxycycline in the treatment of nongonococcal urethritis and mucopurulent cervicitis.to compare the efficacy and tolerability of minocycline versus doxycycline in the treatment of nongonococcal urethritis and mucopurulent cervicitis.19938498758
prevalence of cervical pathogens in women with and without inflammatory changes on smear testing.to assess correlation between nonspecific cervicitis, inflammation, or exudate on cervical smears tests and confirmed presence of known cervical pathogens.19938499824
mycoplasma hominis sternal wound infection and bacteraemia.mycoplasma hominis is a rare cause of bacteraemia in adult males. we believe this report to be the first of mycoplasma hominis bacteraemia and wound infection complicating cardiac surgery. because of difficulties in isolating the organism, cases may be missed. review of the literature on m. hominis bacteraemia in adult males reveals that infection is often mild and most often associated with urethral catheterisation. m. hominis is resistant to many antibiotics including erythromycin. if treatmen ...19938505567
etiology of acute pelvic inflammatory disease proven by laparoscopy.only a few studies have investigated the etiology of acute pelvic inflammatory disease (pid) in laparoscopy-proven cases using pelvic samples for culture. most of these studies were conducted in north america and scandinavia.19938511710
cytoadhesins of mycoplasma hominis.the mechanism of mycoplasma hominis adherence to host cells of the urogenital tract was investigated with monoclonal antibodies (mabs) directed against antigenic surface-localized polypeptides p50, p60, p80, and p100 of cytoadherent m. hominis fbg. a cell enzyme-linked immunosorbent assay was established allowing quantification of cytoadherent mycoplasmas detected by one of the following mabs: four mabs directed against p100 (molecular weight, about 100,000), three mabs against p80, one mab agai ...19938514399
postpartum retroperitoneal abscess due to mycoplasma hominis. 19958527579
ureaplasma urealyticum infection of the placenta in pregnancies that ended prematurely.to investigate the relationship between ureaplasma urealyticum infection of the placenta and premature onset of labor.19968532246
[azithromycin and genital infections].the lower genital tract infections due to chlamydia trachomatis are frequent, essentially occurring in young patients, with possible complications and severe sequela, particularly in women where the sterility risk is one of the major consequences. if an effective treatment could be systematically proposed, a good compliance (easy administration and good toleration) is one of the key factor to success. in this context, the azithromycin displays numerous advantages. the azithromycin in vitro activ ...19958539079
[comparison of 3 culture methods for genital mycoplasmas].to compare and evaluate two commercial methods for mycoplasma hominis and ureaplasma urealyticum with the isolation in agar a7 medium.19958555304
mycoplasma hominis pneumonia. 19938474392
chronic lung disease of preterm infants in finland is not associated with ureaplasma urealyticum colonization.the occurrence of ureaplasma urealyticum and mycoplasma hominis in the airways and the association of these microorganisms with chronic lung disease was studied in preterm infants with a gestational age less than 30 weeks. tracheal aspirates from 49 preterm infants were cultured; 14 (29%) infants were positive for u. urealyticum, and 1 (2%) was positive for m. hominis. of the 16 patients who developed lung disease, 6 (38%) were positive for u. urealyticum, while the expected number of ureaplasma ...19938477168
epidemiologic and molecular investigations of genital mycoplasmas from women and neonates at delivery.the rates of colonization by ureaplasma urealyticum and mycoplasma hominis were evaluated in 208 women at delivery and in their neonates. mycoplasmas were isolated from the cervicovaginal specimens of 100 mothers (48.1%) and from the gastric secretions of 40 neonates (19.2%). the prevalences of u. urealyticum and m. hominis were 47.6% and 11.0% in women and 19.2% and 1.0% in neonates, respectively. premature rupture of membranes was significantly associated with colonization of women by u. ureal ...19958584311
sternotomy infection with mycoplasma hominis in a neonate. 19958584343
efficacy of intravaginal 0.75% metronidazole gel for the treatment of bacterial vaginosis.to assess the efficacy of 0.75% metronidazole vaginal gel, formulated at ph 4.0, for the treatment of bacterial vaginosis in a double-blind, placebo-controlled crossover trial.19938497364
lower genital tract swabs in the prediction of intrauterine infection in preterm prelabour rupture of the membranes.to examine the genital tract flora in women with preterm prelabour rupture of the membranes and to determine the relationship of these microorganisms to those found in fetal blood and amniotic fluid.19968608099
mycoplasma hominis infections occurring in cardiovascular surgical patients.postoperative mycoplasma hominis sternal would or mediastinal infections are uncommon and difficult to diagnose. atypical growth characteristics in routine bacterial culture, and the inability to demonstrate the organism on gram stain, lead to delayed diagnosis of m hominis infections and increased morbidity.19968561647
ureaplasma urealyticum and mycoplasma hominis detected by the polymerase chain reaction in the cervices of women undergoing in vitro fertilization: prevalence and consequences.the prevalence of ureaplasma urealyticum and mycoplasma hominis in the endocervix at the time of oocyte collection in women undergoing in vitro fertilization (ivf) was examined using the polymerase chain reaction (pcr).19958580659
taxonomy of the feline mycoplasmas mycoplasma felifaucium, mycoplasma feliminutum, mycoplasma felis, mycoplasma gateae, mycoplasma leocaptivus, mycoplasma leopharyngis, and mycoplasma simbae by 16s rrna gene sequence comparisons.the nucleotide sequence of the 16s rrna gene of eight mycoplasmas isolated from felids was determined and used for taxonomic comparisons. a signature nucleotide sequence motif and overall sequence similarity to other mollicutes positioned mycoplasma felifaucium, mycoplasma felis, mycoplasma leocaptivus, mycoplasma leopharyngis, and mycoplasma simbae in the mycoplasma fermentans phylogenetic group of mollicutes. mycoplasma arginini and mycoplasma gateae were positioned in the mycoplasma hominis p ...19958590684
analysis of 0.5-kilobase-pair repeats in the mycoplasma hominis lmp gene system and identification of gene products.mycoplasma hominis, an opportunistic pathogenic bacterium of humans, has a small genome of 700 kb. despite this, multiple copies of gene sequences with similarities to the structural gene (lmp1) of a 135-kda surface-located membrane protein (lmp1) have been identified on the genome of m. hominis pg21 (lmp2, lmp3, and lmp4). the distance between the lmp1-lmp2 region and the lmp3-lmp4 region was more than 110 kb. lmp3-lmp4 of m. hominis pg21 was sequenced and found to contain two putative genes. t ...19968631664
bacterial vaginosis in pregnancy: distribution of bacterial species in different gram-stain categories of the vaginal flora.vaginal swabs for microbiological culture were taken from 174 pregnant women whose vaginal flora had been evaluated by gram's stain; 50 had grade iii flora (bacterial vaginosis, bv), 50 grade ii (intermediate), 41 had vaginal flora graded as abnormal which then reverted to grade i (revertants) and 33 had normal flora (controls). the aim was to determine whether bacterial species isolated from women with grade ii flora differed from those with grade iii flora. isolation of lactobacillus spp. decr ...19968683547
[bacteria isolated in 1994-1995 in female upper genitalia infections and in male urethritis. distribution and sensitivity to antibiotics].classify antibiotics according to their individual activity so as to identify those suitable for empiric therapy.19968685180
[does asymptomatic amniotic infection in the second trimester really exist?].our purpose was to evaluate the incidence of asymptomatic amniotic fluid infection.19968690868
comparative in-vitro activity of sparfloxacin against genital pathogens.the in-vitro activity of sparfloxacin against four pathogens commonly implicated in genital infections was compared with that of a number of other commonly administered antimicrobials. sparfloxacin demonstrated excellent activity against neisseria gonorrhoeae (mic range of < or = 0.0002-5 mg/l for beta-lactamase producing strains, and < or = 0.0002-0.03 mg/l for non-beta-lactamase producing strains). this activity was similar to that of lomefloxacin and ciprofloxacin and was greater than that of ...19968737122
[detection of chlamydia trachomatis, mycoplasma hominis and ureaplasma urealiticum in diseases of the male and female reproductive system by using polymerase chain reaction]. 19968744135
[serological survey on mycoplasma infection among three populations in yunnan province].mycoplasma infection in both female and male genital tract has attracted attention recently. 636 infertile and sterile cases together with 284 women with iuds and 113 women with genital inflammatory complications after artificial abortion were detected through indirect hemagglutination test for serological survey on the frequency of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) infection. some of them were detected through isolated culture at the same time. among the 636 infertile and ...19968758403
search for the presence of six mycoplasma species in peripheral blood mononuclear cells of subjects seropositive and seronegative for human immunodeficiency virus.the prevalence of mycoplasma fermentans, mycoplasma pirum, mycoplasma genitalium, mycoplasma pneumoniae, mycoplasma hominis, and mycoplasma penetrans was investigated by using specific pcr assays with peripheral blood mononuclear cells from subjects infected or not infected with the human immunodeficiency virus (hiv). only m. fermentans was detected in 5.8% of 154 hiv-seropositive and 11.1% of 90 hiv-seronegative subjects.19968784596
molecular basis of size and antigenic variation of a mycoplasma hominis adhesin encoded by divergent vaa genes.the molecular basis for the size and antigenic diversity of the variable adherence-associated (vaa) antigen, a major surface protein and a putative adhesin (of mycoplasma hominis, is described. size-variant alleles of the single-copy vaa gene encode abundant surface lipoproteins containing one to four nearly identical, tandem repetitive units of 121 amino acids in the central region of the mature vaa product. gain or loss of central repeats in vaa genes gives rise to distinct size-variant vaa an ...19968698503
amniotic fluid gram stain and leukocyte count in the prediction of intrauterine infection in preterm prelabour amniorrhexis.the purpose of the study was to examine the sensitivity and specificity of the amniotic fluid gram stain and leukocyte count in the prediction of positive fetal blood and amniotic fluid cultures in 80 patients with preterm prelabour amniorrhexis. amniocentesis and cordocentesis were performed and amniotic fluid and fetal blood were cultured for aerobic and anaerobic bacteria. amniotic fluid was also cultured for ureaplasma urealyticum and mycoplasma hominis. the sensitivity of the gram stain in ...19968719713
infection with ureaplasma urealyticum and mycoplasma hominis and the development of chronic lung disease in preterm infants.in a prospective cohort study in a tertiary referral neonatal intensive care unit, the endotracheal secretions of 40 consecutively intubated newborn infants, less than 31 weeks' gestation, were examined weekly for the genital mycoplasmas and all other common bacterial pathogens. fifteen (37%) infants were positive for ureaplasma urealyticum and/or mycoplasma hominis. there were no differences in gestation, birthweight, use of surfactant, or time on ventilator between the culture-positive and neg ...19968740310
development of a diagnostic polymerase chain reaction assay for detection of mycoplasma hominis.a polymerase chain reaction (pcr)-based assay was developed for the detection of mycoplasma hominis. this assay generates a 152-bp pcr product which was part of an initial 471-bp m. hominis genomic dna fragment. the 471-bp dna fragment was shown by hybridization analysis to be unique for m. hominis. the pcr assay can amplify as few as 18 molecules of target dna. this diagnostic assay offers potential for wide clinical application as it is rapid and can be successfully performed on crude sample p ...19958808312
susceptibilities of mycoplasma pneumoniae, mycoplasma hominis, and ureaplasma urealyticum to a new quinolone, trovafloxacin (cp-99,219).the susceptibilities of mycoplasmas to a new quinolone, trovafloxacin (cp-99,219), were compared with those to sparfloxacin and ofloxacin. mycoplasma pneumoniae was as susceptible to trovafloxacin (mic = 0.25 microgram/ml) as to sparfloxacin and fourfold less susceptible to ofloxacin. mycoplasma hominis was highly susceptible to trovafloxacin (mic = 0.06 microgram/ml) and sparfloxacin (mic = 0.03 microgram/ml) and less susceptible to ofloxacin (mic = 0.5 microgram/ml). ureaplasma urealyticum was ...19968849228
dual therapy with doxycycline and ketoprofen compared to doxycycline in the treatment of uncomplicated non-gonococcal urethritis.the aim of this study was to compare a combination of doxycyline and ketoprofen, and doxycycline alone in treating non-gonococcal urethritis (ngu), with particular emphasis on reducing recurrent or persistent disease. sixty-five men with first episode acute ngu were enrolled between november 1992 and november 1994. the men were randomly assigned to either the doxycycline plus ketoprofen group, group a (doxycycline 100 mg twice daily for 10 days plus ketoprofen 200 mg daily for 10 days, n = 34), ...19958849180
phylogeny of some mycoplasmas from ruminants based on 16s rrna sequences and definition of a new cluster within the hominis group.almost complete (> 96%) 16s rrna sequences from nine ruminant mycoplasmas have been determined by solid-phase dna sequencing. polymorphisms were found in four of the 16s rrna sequences, which indicated the existence of two different rrna operons. seven polymorphisms were found in mycoplasma agalatiae, three were found in mycoplasma bovis, one was found in mycoplasma alkalescens, and one was found in mycoplasma bovirhinis. the sequence data were used for construction of phylogenetic trees. all bu ...19968863441
phylogenetic relationships of the porcine mycoplasmas mycoplasma hyosynoviae and mycoplasma hyopharyngis.the phylogenetic positions of the porcine mycoplasmas mycoplasma hyosynoviae and mycoplasma hyopharyngis were determined by using pcr-amplified 16s rrna gene sequences. m. hyosynoviae is a member of the mycoplasma hominis group, while m. hyopharyngis belongs to the mycoplasma fermentans group of mollicutes. neither species is closely related to previously characterized porcine mycoplasmas belonging to the mycoplasma hyorhinis group.19968863455
polymerase chain reaction versus culture for detection of ureaplasma urealyticum and mycoplasma hominis in the urogenital tract of adults and the respiratory tract of newborns.the efficiency of the polymerase chain reaction (pcr) was compared with that of culture for detection of ureaplasma urealyticum and mycoplasma hominis in 726 clinical specimens comprising 189 gynecological samples, 362 urological samples, and 175 samples from newborn infants. the sensitivity of pcr versus culture was 95% for both organisms, while the sensitivity of culture versus pcr was 91% for ureaplasma urealyticum and 84% for mycoplasma hominis. furthermore, pcr tests were faster than cultur ...19968874078
isolation of mycoplasma hominis using the bactec 9000 series blood culture system.mycoplasma hominis has been implicated as an important cause of septicaemia. there have been reported variances in the ability of blood culture systems to support the growth of this organism. in this study the ability of the bactec 9000 series automated system to grow and detect m hominis was assessed. three of five wild m hominis strains grew in the bactec anaerobic plus/f medium but growth was not flagged by the detection mechanism of the system. it is recommended that users of the bactec 9000 ...19968881926
[identification of mycoplasma hominis, ureaplasma urealyticum, and chlamydia trachomatis by polymerase chain reaction]. 19958834213
detection of mycoplasma sp. in bronchoalveolar lavage of aids patients with pulmonary infiltrates.we examined 486 bronchoalveolar lavages (bal) including 32 from aids patients with pulmonary infiltrates and 20 from patients with leukemia or after transplantation. mycoplasmas were found in 4/32 (12.5%) hiv-positive patients compared to 4/454 (< 0.9%) hiv-negative patients (p < 0.001). all of these four hiv-positive patients suffered from advanced infection (cd4 counts < 100/microl) and developed complications (pcp, n = 2, recurrent bacterial pneumonia, n = 1, pulmonary kaposi sarcoma, n = 1). ...19968837371
donor-to-host transmission of mycoplasma hominis in lung allograft recipients.mycoplasma hominis is a significant pathogen in immunocompromised hosts, particularly organ transplant recipients. we describe two recipients of lung allografts from the same donor who had m. hominis pleuropulmonary infection during the immediate postoperative period. the most likely source of infection in these cases was the donor's respiratory tract. the slow-growing pinpoint colonies formed by m. hominis on routine bacterial culture medium may be easily overlooked and should be subcultured to ...19968852981
repetitive elements of the mycoplasma hominis adhesin p50 can be differentiated by monoclonal antibodies.the gene encoding p50, an adhesin of mycoplasma hominis, was identified, cloned, and sequenced. comparison of the derived amino acid sequence with the n-terminal amino acids sequenced by the edman reaction of the native protein revealed that p50 is expressed as a 467-amino-acid precursor. posttranslational modification leads to a 441-amino-acid lipoprotein with an extended, predominantly helical structure and a leucine zipper. computer analysis of the amino acid sequence identified a threefold-r ...19968926064
prevalence of mycoplasma genitalium in men with gonococcal urethritis.a significant association of mycoplasma genitalium with non-gonococcal urethritis has been reported, but the prevalence of this mycoplasma in men with gonococcal urethritis has not been so well studied. in this study, we examined urethral swab specimens from 45 japanese male patients with gonococcal urethritis for the presence of m. genitalium by using a polymerase chain reaction-based assay. we also sought chlamydia trachomatis by an enzyme immunoassay (chlamydiazyme). of the 45 specimens, 2 (4 ...19968940675
sternotomy infection with mycoplasma hominis: a cause of "culture negative" wound infection.wound infections with mycoplasma species are unusual; diagnosis may be delayed because of the growth characteristics of this organism. we report mycoplasma hominis infection of sternotomy wounds in two patients. the first presented with fever and drainage from the incision 1 week after coronary artery bypass grafting. the other patient presented with drainage from the incision three weeks after double-lung transplantation. in both cases, initial cultures were negative, but the typical colonial m ...19968941693
evaluation of commercial kits for quantitative identification and tests on antibiotic susceptibility of genital mycoplasmas.in this study, commercial media and kits for identification and tests for antibiotic susceptibility of genital mycoplasmas were evaluated in comparison to standardized methods. a total of 298 specimens was routinely enrolled. our results showed that all media corresponded for qualitative and semiquantitative identification with a specificity of 100% for both microorganisms and a sensitivity of 94% for u. urealyticum and of 88% for m. hominis. standard methods were superior to commercial kits for ...19968899972
infections due to species of mycoplasma and ureaplasma: an update. 19968909826
high rates of genital mycoplasma infection in the highlands of papua new guinea determined both by culture and by a commercial detection kit.duplicate vaginal swabs were collected from 100 women, and comparisons were made between an in-house broth-agar culture system and a commercially available kit, the mycoplasma ist kit (biomérieux), for the detection of mycoplasma hominis and ureaplasma urealyticum. there was good agreement between the two systems for detection of the genital mycoplasmas in terms of sensitivity, with values of > 92% being obtained. in terms of specificity, the mutual comparisons were less favorable, though specif ...19978968907
a fatal case of mycoplasma hominis meningoencephalitis in a full-term newborn.we report the case of a 20-day-old full-term baby, born to a mother who had had an uncomplicated pregnancy and delivery, who died 13 days after the onset of meningitis. mycoplasma hominis was the sole agent repeatedly recovered from cerebrospinal fluid and from postmortem brain tissue.19978968928
[microbial associations in trichomonas urethritis].the results on isolation of associated microflora from 190 patients suffering from trichomonas urethritis are given. microorganisms belonged to the species: mycoplasma hominis. ureaplasma urealyticum. streptococcus pyogenes. escherichia coli. klebsiella pneumoniae. proteus mirabilis. moraxella lacunata. acinetobacter calcoaceticus and candida. staphylococcus cultures which were isolated from 100% patients belonged to 8 species: staphylococcus aureus, s. intermedius, s. haemolyticus, s. epidermid ...19968983524
detection of mycoplasma hominis and ureaplasma urealyticum in women sexually active or not.genital mycoplasmas play an important role in genitourinary tract disease. the purpose of this study was to isolate m. hominis and u. urealyticum from vaginal and throat swabs and urine from women sexually active or not. samples were taken from women with (cases) or without (controls) genitourinary tract disease and were dipped inoculated into 1 ml of e broth with arginine or urea and ten-fold dilutions were done. samples were incubated at 37 degrees c until phenol red indicator changed to color ...19968986107
[the effect of bacterial polysaccharides on the course of a mycoplasma infection].polysaccharides of clavibacter michiganense have been studied for their effect on mycoplasma infection. it is established that content of t-lymphocytes truthfully increased in the groups of animals whose ps 1 and ps 2 were administered both with protective and medical purpose as compared with that in a group of animals with natural course of mycoplasmosis. under these conditions the effect of polysaccharides on t-helpers were not evidently expressed, while they exerted considerable effect on t-s ...19968991596
common variable immunodeficiency presenting as a mycoplasma hominis septic arthritis.a case is reported of common variable immunodeficiency (cvid) presenting as an acute septic arthritis due to mycoplasma hominis. the diagnosis was not considered until the hypogammaglobulinaemia was discovered and the synovial fluid cultured specifically for mycoplasmas. the importance of diagnosing immunodeficiency states and searching for mycoplasmas in 'bacteriologically culture negative' cases is emphasized.19968945717
characterization of mycoplasma hominis mutations involved in resistance to fluoroquinolones.fluoroquinolone-resistant mutants of mycoplasma hominis were selected in vitro from the pg21 susceptible reference strain either by multistep selection on increasing concentrations of various fluoroquinolones or by one-step selection on agar medium with ofloxacin. the quinolone resistance-determining regions (qrdr) of the structural genes encoding the a and b subunits of dna gyrase were amplified by pcr, and the nucleotide sequences of eight multistep-selected resistant strains were compared to ...19979021178
sexual risk behaviour in women carriers of mycoplasma hominis.to study the sexual behaviour of women harbouring mycoplasma hominis in the vagina.19968917001
[the spread of mycoplasma infections]. 19969026047
[chromosome aberrations induced by mycoplasma infections in human peripheral blood lymphocytes].it is shown that in vitro infection of human peripheral blood lymphocytes by mycoplasmas (acholeplasma laid-lawii pg8 and mycoplasma hominis pg21) leads to multiple chromosome aberrations, predominantly to chromatid gaps and breaks. oxidative stress in the infected cells is regarded as one of the factors causing chromosome damage.19968964467
mycoplasma hominis meningitis complicating head trauma: case report and review. 19979114165
isolation of mycoplasma hominis on cdc anaerobic blood agar. 19968997570
vaginal flora changes associated with mycoplasma hominis.the aim of this study was to investigate any association between vaginal carriage of mycoplasma hominis and genital signs and symptoms, other microbial findings, and some risk behavior factors in women with and without bacterial vaginosis.19979024109
[urogenital infections linked to chlamydia and mycoplasmas].chlamydia trachomatis is responsible for urogenital infections, often minimally symptomatic, revealed by their complications. mycoplasmas, mainly ureaplasma urealyticum and mycoplasma hominis, are normal commensal organisms of the genital tract, which sometimes makes it difficult to determine their pathogenicity. however, they are responsible for urogenital infections, and u. urealyticum is a pathogen in male urethris. the laboratory diagnosis is based on specific techniques adapted to these mic ...19979116732
the mycoplasma hominis p120 membrane protein contains a 216 amino acid hypervariable domain that is recognized by the human humoral immune response.in the antigenically heterogeneous species mycoplasma hominis a monoclonal antibody, mab 26.7d, was previously found to recognize a 120 kda polypeptide from m. hominis 7488. this antibody did not react with the type strain pg21. the homologous gene from m. hominis pg21 was cloned and sequenced and found to have a sequence identity of 91% with the gene of strain 7488. one hypervariable and two semivariable regions were detected. the epitope for mab 26.7d was mapped to the hypervariable domain by ...19979043143
surgical infections with mycoplasma: a brief review.mycoplasma hominis and ureaplasma urealyticum are common inhabitants of the human genital tract. evidence for an aetiological role in pyelonephritis, pelvic inflammatory disease, post-abortion and post-partum fever has been presented. there are sporadic reports of mycoplasma causing serious extragenital infection such as septicemia, septic arthritis, neonatal meningitis and encephalitis. we review 38 cases of surgical infections with mycoplasma.19979114682
extragenital mycoplasma hominis infection in two liver transplant recipients. 19979114209
isolation of mycoplasma hominis from a brain abscess.mycoplasma hominis is a commensal in the genital tract of women and has been associated with urogenital and extragenital infections. however, central nervous system infections with this organism in adults are very rare. here we describe the recovery of m. hominis from a brain abscess associated with a postpartum infection. seroconversion to the isolated strain was detected by both a metabolic inhibition test and an immunoblotting assay. this case demonstrates the pathogenic potential of m. homin ...19979157171
systematic detection of mycoplasmas by culture and polymerase chain reaction (pcr) procedures in 209 synovial fluid samples.the objective was to investigate the presence of mycoplasmas in rheumatoid arthritis (ra) and other chronic arthritides. samples of synovial fluid (sf) were systematically collected from all patients presenting with an articular effusion. each sample was divided into three parts. the first was kept for cytological count and culture on standard media for pyogens and mycobacteria, the second was cultivated on specific media for mycoplasmas and the third frozen for subsequent study by polymerase ch ...19979133961
[screening of sexually transmitted diseases (mycoplasma hominis, ureaplasma urealyticum and chlamydia trachomatis) in young women].the author searched for sexually transmitted ureaplasma urealyticum, mycoplasma hominis and chlamydia trachomatis infection among young females under 30. out of the 400 women examined (including 86 primigravidas) 46 percent, and out of the pregnant women 52.3 percent proved to be colonized by one of the above pathogens. 11 percent carried mycoplasma hominis, 44.75 percent ureaplasma urealyticum and 7.75 percent chlamydia trachomatis. positivity was more frequent among those having several partne ...19979173376
[mycoplasma hominis. a rare causative agent of acute pyelonephritis].a 23-year-old woman was admitted with typical signs of an acute urinary tract infection: fever, pain on tapping over both renal areas and in both flanks, urgency and dysuria. she had a history of renal colic with spontaneous passage of a renal stone.19979190301
evaluation of the etest for detection of tetracycline resistance in mycoplasma hominis.the etest was compared with microbroth dilution for performing in vitro susceptibility tests in 38 isolates of mycoplasma hominis chosen to represent a wide range of mic values. mic50s were 4 micrograms/ml for both methods; whereas, mic90s were 64 and > 256 micrograms/ml for broth and etest, respectively. etest mics determined on sp 4 agar were usually two or more dilutions greater than microbroth mics in sp 4 broth, and values were prone to change by one to two dilutions when different inoculum ...19979154407
molecular characterization of a gene encoding a membrane protein of spiroplasma citri.a 9.6-kb genomic dna segment, previously cloned from the phytopathogen spiroplasma citri br3-3x [fletcher et al. (1981) phytopathology 71, 1073-1080], contained several open reading frames including one encoding a 58-kda protein. in this work, the transcription initiation site of the p58 mrna was mapped and part of the gene was expressed in escherichia coli as a fusion protein. a synthetic peptide, whose sequence is included in the fusion protein, was produced. antibodies against both the fusion ...19979161418
mycoplasma in urine and blood following catheterisation of patients undergoing vascular surgery.the purpose of this investigation was to determine if mycoplasmas enter the bloodstream after urinary tract catheterisation in patients undergoing vascular surgery in order to evaluate the efficiency of the routine prophylactic antibiotic treatment.19979267343
what is normal vaginal flora?to observe the composition of the vaginal flora of healthy women over time, and in relation to hormonal changes, sexual activity, and hygiene habits.19979155551
[electron microscopic and immunomorphologic study of the placenta in genital mycoplasmosis].peculiar ultrastructural features allowed the authors to reveal m. homminis corpuscles in placental tissues of women with genital mycoplasmosis. mycoplasma was found in the amniotic epithelium, chorionic plate, in the lumen of villous capillaries, this showing possible hematogenic way of the infection from mother to fetus. in the placenta of women with genital mycoplasmosis immunopathological processes develop in association with formation of pathogenic immune complexes (pic) that are fixing the ...19979206953
mycoplasma infection and rheumatoid arthritis: analysis of their relationship using immunoblotting and an ultrasensitive polymerase chain reaction detection method.to examine the relationship between infection with mycoplasma and the development of rheumatoid arthritis (ra) and juvenile rheumatoid arthritis (jra).19979214421
[bacterial flora of genital organs in women treated for infertility].bacterial flora in 72 women suffering from infertility was assessed. the medium age of women was 28.9 years (22-37) and the duration of infertility-5.5 years (2-15). cultures were taken during laparoscopy (65) or microsurgery (7) from vagina, cervical canal and the fallopian tubes. specific cultures for anaerobic and aerobic bacteria, n. gonorrhoeae, c. trachomatis and mycoplasma hominis were taken. in the specimens from fallopian tubes 21 species of bacteria were cultured. the most often coagul ...19979296942
mycoplasma hominis and ureaplasma urealyticum in patients with sexually transmitted diseases.mycoplasma hominis and ureaplasma urealyticum can be isolated with considerable frequency from the human urogenital tract and are thought to cause various syndromes such as nongonococcal urethritis, pelvic inflammatory disease, pyelonephritis or infertility. the aim of this study was the evaluation of the presence of different genital pathogens in patients with sexually transmitted diseases (std) and, in particular, the detection of mycoplasmas in individuals infected with genital microbes and a ...19979286064
detection of urogenital mycoplasmal infections in primates by use of polymerase chain reaction.urogenital mycoplasmal infections could affect use of primates as models for reproductive system studies and could affect reproduction in captive primates, but could be useful as animal models of similar human infections. we conducted a pilot study to assess detection of urogenital mycoplasmal infections in primates by use of polymerase chain reaction (pcr). healthy animals were anesthetized, and vaginal, cervical, or endometrial and urethral swab specimens were collected from females and males, ...19979355087
localized reversible frameshift mutation in an adhesin gene confers a phase-variable adherence phenotype in mycoplasma.the variable adherence-associated (vaa) antigen of mycoplasma hominis is an abundant surface lipoprotein adhesin that may mediate important interactions of this wall-less prokaryotic pathogen with the human host. extensive mutational variation of vaa size, as well as sequence and antigenic divergence, has been described previously. using a series of clonal isolates representing an isogenic lineage of variants oscillating in vaa expression, vaa is further shown in this study to undergo high-frequ ...19979364912
mycoplasma hominis pneumonia complicating bilateral lung transplantation: case report and review of the literature.mycoplasma hominis is a commensal of humans. the organism has been predominantly associated with infections of the genitourinary tract. extragenital infections have been described in neonates, in women during the postpartum period, and in immunocompromised patients. pneumonia caused by m. hominis is very rare. this report describes the development of m. hominis pneumonia in a lung transplantation recipient and underscores the difficulty in establishing the correct diagnosis and the need for earl ...19979367488
molecular biology of mycoplasma.mycoplasmas are the smallest free living microorganisms with the smallest genome. the g+c content is in general low (25-33%) and the coding capacity is about 600 proteins. mycoplasma species are phylogenetically related, they use the genetic codon uga for tryptophan, and show rapid evolution, with a high rate of divergence. the genomes of mycoplasma genitalium and mycoplasma pneumoniae have been fully sequenced. striking features of the m. genitalium sequencing project are the presence of a high ...19979286059
genital mycoplasma infections.since 1937, 13 mycoplasma species, two acholeplasma species, and one ureaplasma species have been isolated from humans. six of these have the urogenital tract as the primary site of colonisation but others, which have the oropharynx and respiratory tract as the primary site, are found occasionally in the urogenital tract because of orogenital contact. mycoplasma hominis was the first to be isolated and is most strongly associated with bacterial vaginosis (bv), together with a variety of other ba ...19979286063
in-vitro susceptibilities of mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum to clinafloxacin, pd 131628, ciprofloxacin and comparator drugs. 19979302005
[the role of chlamydia trachomatis infections in women with urinary tract diseases].the aim of our study was to determine the incidence of chlamydia trachomatis (c. trachomatis) infections and its role in the etiopathogenesis of urinary tract infections in women. we studied 68 women, who where divided into two groups (i-with urethral syndrome, ii-with other urologic diseases). with the use of direct diagnostic methods (immunofluorescence method, tissue culture) c. trachomatis infection was found in 25/68 (36.8%) women. in the group of women with urethral syndrome the percentage ...19979377664
cytokine and prostaglandin production by amnion cells in response to the addition of different bacteria.our goal was to evaluate the effect of escherichia coli, bacteroides fragilis, mycoplasma hominis, and staphylococcus aureus on cytokine and prostaglandin production by amnion cells in vitro.19989465802
[inflammatory smears in cervicovaginal cytology. a finding meaning infection?].inflammatory changes are relatively frequent findings in cervical smears and are generally believed to be a consequence of genital infection. however, clinical signs of infection are frequently absent and no consensus exists on the management of these patients. the objective of this study was to assess whether or not inflammatory smears are exclusively a consequence of genital infection. for this purpose, the prevalence of genital infection in a group of women with inflammatory cells in the cerv ...19979477586
[new data on the etiological factors in acute pyelonephritis].spectrum of microbe agents of acute pyelonephritis has been studied. it is shown that mycoplasma hominis plays an important role in etiology of acute pyelonephritis besides the already known enterobacteria and gram-positive cocci. it is also established that mycoplasmal pyelonephritis more often develops in patients with the diseases of urinary blade (cystitis) and genital organs. results of serological investigations which confirm etiological role of isolated microorganisms in the initiation of ...19979480016
induction of neutrophil chemoattractant cytokines by mycoplasma hominis in alveolar type ii cells.bronchopulmonary dysplasia (bpd) is a chronic lung disease of premature infants who are mechanically ventilated due to respiratory distress. the disease consists of an initial inflammatory influx of neutrophils to the lungs, followed by long-term chronic fibrosis of the lung tissue. the antigenic repertoire that initiates the inflammatory component of bpd has not been defined. furthermore, the repertoire of cytokines responsible for attracting neutrophils to the lung and the mediators of pathoge ...19979393806
vaginal flora changes associated with mycoplasma hominis. 19989500509
therapy of bacterial vaginosis. 19989511031
a review of the in-vitro activity of quinupristin/dalfopristin against intracellular pathogens and mycoplasmas.quinupristin/dalfopristin displays in-vitro bacteriostatic activity against all legionella spp. (mics = 0.06-2 mg/l), with legionella pneumophila usually being at least two-fold more sensitive to quinupristin/dalfopristin than legionella bozemanii, legionella dumoffii, legionella gormanii and legionella micdadei (mic = 0.06-2 vs 1-2 mg/l, respectively). against legionella spp., quinupristin/dalfopristin was at least as active as erythromycin. quinupristin/dalfopristin was active in vitro against ...19979511064
the in-vitro activity of grepafloxacin against chlamydia spp., mycoplasma spp., ureaplasma urealyticum and legionella spp.the activity of grepafloxacin, a new orally active fluoroquinolone, was compared with the activities of ofloxacin, clarithromycin and doxycycline against chlamydia pneumoniae, chlamydia trachomatis, mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum, and with the activities of ofloxacin, clarithromycin and rifampicin against legionella spp. grepafloxacin (mic range 0.06-0.12 mg/l) was some 8-16 times more active than ofloxacin against the chlamydiae, showing activity similar to ...19979484871
[presence of urogenital mycoplasma in fertile women]. 19979522530
a gtp-binding protein of mycoplasma hominis: a small sized homolog to the signal recognition particle receptor ftsy.a protein homologous to the escherichia coli ftsy which in turn has characteristics in common with the alpha-subunit of the eukaryotic signal recognition particle receptor (sralpha) in the membrane of the endoplasmic reticulum, was identified in mycoplasma hominis and its encoding dna sequenced. the aa similarity to e. coli ftsy and b. subtilis ftsy was 38% and 51%, respectively. the protein was synthesized in e. coli, purified and shown to bind gtp. subcellular localization studies revealed tha ...19979409769
few microorganisms associated with bacterial vaginosis may constitute the pathologic core: a population-based microbiologic study among 3596 pregnant women.to evaluate the association between various microorganisms isolated from the genital tract in pregnant women with bacterial vaginosis.19989539529
sequences of the 16s rrna genes and phylogeny of the goat mycoplasmas mycoplasma adleri, mycoplasma auris, mycoplasma cottewii and mycoplasma yeatsii.the nucleotide sequences of the 16s rrna genes from the type strains of four goat mycoplasmas, mycoplasma adleri, mycoplasma auris, mycoplasma cottewii and mycoplasma yeatsii, were determined by direct solid-phase dna sequencing. polymorphisms were found in two of the 16s rrna gene sequences, showing the existence of two different rrna operons. three polymorphisms were found in m. adleri, and one was found in m. yeatsii. the sequence information was used for the construction of phylogenetic tree ...19989542096
[severe genital mycoplasma infection following cesarean section].authors report a serious case of post-caesarean delivery endometritis caused, probably exclusively, by genital mycoplasmas: ureaplasma urealyticum and mycoplasma hominis. the initial treatment of the patient with various penicillins, ceftriaxone, gentamicin, metronidazole and nystatine proved ineffective. subsequently, as microbiological tests turned out positive for genital mycoplasmas, a therapy of doxycyclin was introduced and a full recovery could be attained. authors' experience is consiste ...19989545798
septic arthritis by mycoplasma hominis: a case report and review of the medical literature. 19979462180
mycoplasma hominis expresses two variants of a cell-surface protein, one a lipoprotein, and one not.a protein similar to the previously characterized variable surface-exposed membrane protein p120 was identified (p120'), establishing that mycoplasma hominis pg21 possesses a novel gene family. the gene, p120', was sequenced and found to have some distinctive properties including a putative start codon of gtg, rather than the common atg codon, and a coding region with a high g + c content, characteristic of essential housekeeping genes in mycoplasmas. no sequence homology was found to known prot ...19989534245
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