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quinolones as an alternative treatment of chlamydial, mycoplasma and gonococcal urogenital infections.we report the results of several trials aimed at evaluating the quinolones in urogenital infections. in chlamydia trachomatis infections, ofloxacin (200 mg b.i.d. for 10 days) gave a cure rate of 98% (n = 66), and fleroxacin (400 mg s.i.d. for 7 days) provided a cure rate of 89% (n = 19). a double-blind study comparing fleroxacin (600 mg s.i.d.) to doxycycline (100 mg b.i.d.) for 7 days showed similar high cure rates for both regimens (100%; n = 23). in mycoplasma hominis infections, ofloxacin ( ...19921421625
in vitro activity of several antimicrobial agents against genital mycoplasmas.the increasing resistance of genital mycoplasmas to tetracyclines is a serious problem, since this group of antibiotics is one of the few that is effective against virtually all species of mycoplasmas. tetracyclines are also used to treat many sexually transmitted diseases. in this study, we assessed the susceptibility of mycoplasma hominis and ureaplasma urealyticum to macrolides, tetracyclines, spectinomycin, and trospectomycin by the agar dilution method. for m hominis, trospectomycin was the ...19921468088
[immunoreactivity of mycoplasma hominis pg21 antigens and human sperm antigens].the authors selected from the who bank for reproductive immunology ten sera containing concurrently antispermatozoal antibodies and antibodies against mycoplasma hominis pg21. by absorption examination of selective sera with a different concentration (10(9)/ml, 3.10(8)/ml, 10(8)/ml) of human spermatozoa and a rinsed sonicate of mycoplasma hominis pg21 in dilutions of 1:20, 1:80, 1:320 the authors did not find a crossed immunoreactivity between sperm antigens and mycoplasmic antigens.19921473166
a longitudinal study of pelvic inflammatory disease.to study the microbiology and long term prognosis of pelvic inflammatory disease (pid).19921477024
gardnerella vaginalis: characteristics, clinical considerations, and controversies.the clinical significance, gram stain reaction, and genus affiliation of gardnerella vaginalis have been controversial since gardner and dukes described the organism as the cause of "nonspecific vaginitis," a common disease of women which is now called bacterial vaginosis. the organism was named g. vaginalis when taxonomic studies showed that it was unrelated to bacteria in various genera including haemophilus and corynebacterium. electron microscopy and chemical analyses have elucidated the org ...19921498765
mycoplasma hominis in women with bacterial vaginosis.an association between m. hominis and bacterial vaginosis (bv) was looked for in total 205 women characterized as having bv. vaginal specimens from these patients were studied for the presence of amines, polymorphonuclear leukocytes, clue cells, and lactobacilli, and were also cultured to isolate gardnerella vaginalis, m. hominis, trichomonas vaginalis, candida albicans and anaerobes. g. vaginalis was found to be the most common isolate from 132 cases (64.3%). m. hominis was isolated from 90 cas ...19921506065
septic arthritis and bacteremia due to mycoplasma resistant to antimicrobial therapy in a patient with systemic lupus erythematosus.we report a case of septic arthritis caused by mycoplasma hominis in a patient with systemic lupus erythematosus. the infection started as monarthritis but spread to at least four joints despite apparently suitable therapy with various antimicrobial agents, including doxycycline, clindamycin, and ciprofloxacin; subsequent bacteremia was documented. control was ultimately achieved with use of the experimental fluoroquinolone temafloxacin in combination with doxycycline administration, arthroscopi ...19921520784
prerequisite work-up of the couple before in-vitro fertilization.prerequisite examinations, including immune status for rubella and hepatitis b antigens, cultures for chlamydia trachomatis and mycoplasmas, pap smear and hystersalpingogram were performed in 227 couples before their enrollment into an in-vitro fertilization (ivf) programme. the examinations were completed in 187 couples. immune status for rubella had already been documented in 45% of the women: of the remainder, 11 patients were not immune (6%). a screening test for hepatitis b antigens had alr ...19921522189
nucleotide sequence of the arginine deiminase gene of mycoplasma hominis.the arginine deiminase gene of mycoplasma hominis was amplified by the polymerase chain reaction, and its entire nucleotide sequence was determined. this gene consists of 1227 base pairs encoding 409 amino acids, and has 35.2% guanine plus cytosine content. nucleotide sequence homologies of the arginine deiminase gene between m. hominis and m. arginini, and between m. hominis and m. orale were 82.1 and 80.8%, respectively, suggesting that this gene is highly conserved among arginine-utilizing my ...19921522817
rapid detection of tetm in mycoplasma hominis and ureaplasma urealyticum by pcr: tetm confers resistance to tetracycline but not necessarily to doxycycline.tetracycline resistance in mycoplasma hominis and ureaplasma urealyticum has been associated with the tetm determinant and has recently been increasing in incidence. we report here a rapid method for detection of the tetm determinant based on the use of the polymerase chain reaction (pcr) to amplify a 397-bp dna fragment from the tetm gene and verification of specificity using the restriction enzyme taqi. analysis of 42 u. urealyticum and 49 m. hominis isolates indicates that the pcr method may ...19921526460
lumbar pain caused by mycoplasma infection.a 45-year-old woman was admitted to hospital following acute onset of lower back pain. clinical and laboratory investigations established a lumbar paraspinal soft tissue infection with mycoplasma hominis associated with severe spondylarthrosis at l5/s1. a relationship to a recently performed hysterectomy must be considered.19921533852
the prevalence and clinical significance of intraamniotic infection with candida species in women with preterm labor.intraamniotic infection is considered a major etiologic factor of preterm birth. positive amniotic fluid cultures are rarely contaminated with candida species. the presence of this microorganism is associated with a poor pregnancy outcome. out of 773 transabdominal amniocenteses performed in women presenting with preterm labor and intact membranes, 77 patients (9.9%) had positive amniotic fluid cultures and in 5 women (6.5%) candida species were identified. on the other hand, 625 amniocenteses w ...19921550392
physical and genetic mapping of the genomes of five mycoplasma hominis strains by pulsed-field gel electrophoresis.we present the complete maps of five mycoplasma hominis genomes, including a detailed restriction map and the locations of a number of genetic loci. the restriction fragments were resolved by field inversion gel electrophoresis or by the contour-clamped homogeneous-electric-field system of pulsed-field gel electrophoresis. all the apai, smai, bamhi, xhoi, and sali restriction sites (total of 21 to 33 sites in each strain) were placed on the physical map, yielding an average resolution of 26 kb. ...19921551841
adverse outcome in pregnancy following amniotic fluid isolation of ureaplasma urealyticum.infections in pregnancy with ureaplasma urealyticum have been associated with a wide range of adverse outcomes, such as early abortion, stillbirth, prematurity, and neonatal morbidity and mortality. causality has been difficult to demonstrate secondary to the high prevalence of asymptomatic lower genital tract (lgt) colonization and culture data from inaccessible or potentially contaminated sites. between 1985 and 1989, 2461 second-trimester genetic amniocenteses were evaluated at the cytogeneti ...19921553356
ureaplasma urealyticum and mycoplasma hominis in women with systemic lupus erythematosus.to determine the prevalence of genitourinary mycoplasma infection in women with systemic lupus erythematosus (sle).19921567492
[role of mycoplasmas in the last month of pregnancy and postpartum pathology. prospective study of 577 pregnancies].the authors report the findings of a prospective study of 577 pregnancies. the purpose of this study was to determine the influence of mycoplasma on peri-post-natal illness arising during the last month of gestation and during the immediate post-partum period. the incidence of contamination was 29% of ureaplasma urealyticum and 2.3% for mycoplasma hominis. this showed little change post partum. the rate of transmission to the fetus or placenta is low. no difference in end-of-term fetal weight wa ...19921579798
comparison of commercially available media for detection and isolation of ureaplasma urealyticum and mycoplasma hominis.the mycotrim triphasic flask system (irvine scientific, irvine, calif.) was compared with a system composed of mycotrim gu broth (irvine scientific) and a7 or a8 agar (remel, lenexa, kans.) for the ability to detect ureaplasma urealyticum and mycoplasma hominis from 129 genital specimens. of the 64 specimens positive for u. urealyticum, 25, 98, and 100% were detected on mycotrim triphasic agar and a7 and a8 agars, respectively. all 18 specimens that grew m. hominis were detected by a7 and a8 aga ...19921583143
ovarian actinomycosis presenting as acute peritonitis.a patient with acute peritonitis due to ovarian actinomycosis and the association of this disease with the intrauterine contraceptive device is discussed. because adequate treatment requires prolonged antibiotics the diagnosis should be sure. unfortunately if antibiotics are given pre-operatively culture is usually unsuccessful and thus careful examination of all removed material is essential. as many of these women are of child bearing age surgical intervention should aim to preserve fertility ...19921586308
[ureaplasma urealyticum and mycoplasma hominis: incidence and clinical significance of their isolation in the perinatal period].in order to known the clinical significance of the colonization of the maternal genitalia and infant with genital mycoplasmas, we studied prospectively 219 pairs of mothers and newborns admitted for delivery to a general public hospital. u. urealyticum and m. hominis were isolated from 72 (32.9%) and 10 (4.6%) of the mothers, respectively. during the first 4 days of life. u. urealyticum and m. hominis were isolated from 23 (10.5%) and 1 (0.5%), of the newborn infants respectively. maternal colon ...19921605412
comparison of ofloxacin and metronidazole for the treatment of bacterial vaginosis.the efficacy and safety of ofloxacin, 200 mg twice daily for 7 days, was compared with metronidazole, 400 mg twice daily for 7 days, for the treatment of bacterial vaginosis (bv). diagnosis of bv was confirmed by at least 3 of the following 4 criteria: the presence of an abnormal vaginal discharge on examination, clue cells on microscopy of vaginal specimens, vaginal ph greater than 5.0 and a positive amine test. vaginal specimens were examined for mobiluncus spp, analysed for the succinate/lact ...19921616967
a randomized, comparative study of the efficacy and tolerance of roxithromycin and doxycycline in the treatment of women with positive endocervical cultures for chlamydia trachomatis and mycoplasma spp. in an in vitro fertilization program.a total of 45 patients wishing to enter an in vitro fertilization program had positive endocervical cultures for chlamydia trachomatis, 86 had positive cultures for ureaplasma urealyticum, and 11 had positive cultures for mycoplasma hominis. these patients and their consorts were randomly allocated to treatment with either roxithromycin or doxycycline. both antibiotics were completely effective against c. trachomatis. in patients with u. urealyticum infection, roxithromycin was successful in 73% ...19921617924
microbial causative agents of male urethritis.the incidence of chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, neisseria gonorrhoeae and trichomonas vaginalis was studied in men with urethritis. out of 150 examined men 48.7% had the positive isolation of u. urealyticum, 26.0% c. trachomatis, 22.7% n. gonorrhoeae, 18.7% m. hominis and in one (0.7%) patient t. vaginalis was found. none of the above mentioned microorganisms was detected in 24.7% of examined men. in 43.3% cases only one agent was isolated. in 23.3% of the men ...19921619287
[mycoplasmas and pregnancy. preliminary study].the prevalence of genital mycoplasmas was studied among 191 pregnant women followed up at diaconesses hospital, paris. ureaplasma urealyticum was recovered from 65% of patients, alone (54%) or in association with mycoplasma hominis (11%). the relationship to pregnancy outcome, the effect of erythromycin treatment, the interest of biological markers of infection (mycoplasmal quantitation and serologic tests, c-reactive protein) and the role of other known genital pathogens were investigated. vagi ...19921624724
detection of mycoplasma contamination through modulation (stimulation or inhibition) of thymidine incorporation by unstimulated mouse spleen cells.in the present report we describe a rapid and sensitive assay for mycoplasma detection in cell cultures. the assay is based on the ability of contaminated culture supernatants to modulate [3h]tdr incorporation by unstimulated mouse splenocytes. several mycoplasma species (mycoplasma orale, culturable and non-culturable strains of mycoplasma hyorhinis) inhibited [3h]tdr incorporation and permitted the detection of some contaminated cell cultures that would otherwise have escaped detection in assa ...19921640109
the in vitro activity of some 14-, 15- and 16- membered macrolides against staphylococcus spp., legionella spp., mycoplasma spp. and ureaplasma urealyticum.erythromycin is a macrolide antimicrobial chemically comprised of a 14-membered lactone ring substituted with a neutral (cladinose) and an amino (desosamine) sugar. recently, a number of new macrolide molecules have been identified containing either 14-, 15- or 16-membered substituted lactone rings. in this study the authors have determined the in vitro activity of roxithromycin and clarithromycin (both 14-membered macrolides), azithromycin (a 15-membered macrolide or azalide) and midecamycin ac ...19911650694
incidence of nonbacterial intraamniotic infections in abnormal pregnancies.amniotic fluids from 41 abnormal and 47 control pregnancies were examined for viruses, chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis. abnormal pregnancies included those with fetal abnormalities, maternal complications, suspected chorioamnionitis and premature labor. two percent of cultures were positive, including cytomegalovirus from one case of fetal growth retardation and u urealyticum from two patients with chorioamnionitis and from two controls. the abnormal pregnanc ...19911662719
fluoroquinolones for the treatment of nongonococcal urethritis/cervicitis.approximately 50% of cases of nongonococcal urethritis reported among men are caused by chlamydia trachomatis. other pathogens implicated in the bacterial etiology of nongonococcal urethritis that occur independently or concurrently with gonorrhea include ureaplasma urealyticum and species of mycoplasma, including mycoplasma hominis. the etiology of up to half of the cases, however, remains uncertain. historically, c. trachomatis genital infections have been difficult to diagnose because of the ...19911662887
temafloxacin: an overview.temafloxacin (6-fluoro-7-piperazino-4-quinolone) is a new fluoroquinolone with a 7-8 hour half-life and rapid gastrointestinal absorption. these characteristics make it an ideal antimicrobial for once- or twice-daily oral dosing. with the exception of the central nervous system (cns), temafloxacin has excellent tissue and body fluid penetration and concentration. temafloxacin has broad antimicrobial activity against gram-positive and gram-negative bacteria, including improved in vitro activity a ...19911662889
in vitro activity of temafloxacin against pathogens causing sexually transmitted diseases.the fluoroquinolones represent a new therapeutic alternative for the treatment of many sexually transmitted diseases, especially those caused by neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and urea-plasma urealyticum. in vitro studies have demonstrated the 90% minimal inhibitory concentrations (mic90s) of temafloxacin for these pathogens to be less than 0.03 micrograms/ml for n. gonorrhoeae; 0.25 micrograms/ml for c. trachomatis; 0.125-2 micrograms/ml for m. hominis; and 4 ...19911662891
fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody.the detection of mycoplasmas in cell cultures is still a problem, especially in those laboratories in which the detection and identification of microorganisms is not established as a routine procedure. in our laboratory, monoclonal antibodies (mabs) have been prepared to acholeplasma laidlawii, mycoplasma hyorhinis, mycoplasma orale, mycoplasma arginini and mycoplasma salivarium. 30 mabs were obtained and one of these, designated ccm-2, was shown to bind to all five mycoplasma species. it also b ...19901697315
a high-affinity human monoclonal igm antibody reacting with multiple strains of mycoplasma hominis.human monoclonal antibodies were produced against mycoplasma hominis by in vitro immunization of peripheral blood lymphocytes from a healthy seropositive donor using low amounts of antigen (5 ng/ml). the immune b lymphocytes were subsequently immortalized by epstein-barr virus transformation followed by somatic cell fusion. using this procedure, several specific anti-m. hominis monoclonal antibodies of mu isotype were obtained exhibiting apparent affinity constants as high as 3 x 10(9)m-1. the p ...19901700092
monoclonal antibodies to surface antigens of a pathogenic mycoplasma hominis strain.three monoclonal antibodies (mabs) were prepared against an arthritogenic strain of mycoplasma hominis isolated from the joint aspirates of a patient with chronic septic arthritis. immunoblots of polyacrylamide gel-electrophoresed proteins before and after surface proteolysis showed that the predominant antigenic determinants were on surface-exposed polypeptides. these polypeptides have extensive hydrophobic characteristics, as demonstrated by triton x-114 phase partitioning. the electrophoresed ...19911708355
mycoplasma hominis infection of perihepatic hematomas in a liver transplant recipient.we report the case of a recipient of liver transplantation in whom postoperative perihepatic hematomas were infected by mycoplasma hominis. etiologic diagnosis was delayed because this organism is a rare cause of postoperative infection and usually does not grow on standard bacteriologic media. the role of m. hominis in postoperative infections and the diagnostic problems of this organism are discussed.19921728082
the prevalence of potential pathogenic micro-organisms in the endocervix of pregnant women at tygerberg hospital.the prevalence of potential pathogenic organisms cultured from the endocervix in 206 pregnant patients was: ureaplasma urealyticum 63%, mycoplasma hominis 39%, candida albicans 20%, chlamydia trachomatis 11%, neisseria gonorrhoeae 7%, group b beta-haemolytic streptococcus 6% and listeria monocytogenes 1%. the most common complications of pregnancy found were: preterm labour 14%, urinary tract infection 8% and premature rupture of membranes 4%. significantly more patients in whom c. trachomatis o ...19921734556
the relationship of hydrogen peroxide-producing lactobacilli to bacterial vaginosis and genital microflora in pregnant women.lactobacilli provide an important microbial defense against genital colonization by pathogens. the role of hydrogen peroxide (h2o2) in the control of genital microflora was explored in a cross-sectional study of 275 women in the second trimester of pregnancy. vaginal cultures were obtained for detection of h2o2-positive and h2o2-negative lactobacilli and other members of the genital microflora. compared with women with h2o2-negative lactobacilli, women colonized by h2o2-positive lactobacilli wer ...19921738516
[value and limits of research on mycoplasma hominis and ureaplasma urealyticum in the gastric fluid of newborn infants].mycoplasma hominis and ureaplasma urealyticum were cultured and counted in the gastric fluid of 153 neonates divided into three groups: 28 preterm neonates managed in an intensive care unit (group i); 83 full term neonates with suspected infection (group ii); and 42 full term neonates with no evidence of infection (group iii). the colonization rate (17.85%) in the intensive care unit group was not significantly different from the rates seen in the two other groups. these results do not militate ...19911750746
mycoplasma hominis infection of a breast prosthesis. 19911753125
[ureaplasma urealyticum and mycoplasma hominis in male urethritis].we conducted a multi-center clinical study to evaluate the role of ureaplasma urealyticum and mycoplasma hominis in male urethritis. the incidence of each organism in first-voided urine samples of 160 male urethritis patients, including 28 with gonococcal and 126 with non-gonococcal urethritis, was investigated. u. urealyticum and m. hominis were isolated from 13.6% and 6.5%, respectively, of the urine samples, and the concentration of each mycoplasma exceeded 10(3) ccu/ml (color changing units/ ...19911755423
detection of variable dna repeats in diverse eukaryotic microorganisms by a single set of polymerase chain reaction primers.we cloned and sequenced a variable dna repeat from trichomonas vaginalis, a flagellated protozoan parasite. targeting of this repeat in the polymerase chain reaction resulted in complex and intense product patterns for a wide variety of eukaryotic microorganisms, including the pathogenic protozoan parasites t. vaginalis, giardia lamblia, leishmania donovani, three species of trypanosoma, and four species of acanthamoeba; the nonpathogenic protozoans, paramecium tetraurelia and tetrahymena thermo ...19911757544
detection of mycoplasma hominis antibodies by three different techniques, application to women with pelvic inflammatory disease.mycoplasma hominis, a mycoplasma potentially isolated from the genital tract of healthy women, seems to have some role in pelvic inflammatory diseases (pid). three serologic techniques were developed to detect antibodies to m. hominis: elisa (igg, igm), microimmunofluorescence (mif) and western blot. rabbit and human control sera were used to establish the standard assay conditions. elisa proved to be more sensitive but less specific than mif. cross-reactivity with antibodies to m. pneumoniae ex ...19911759730
[identification of mycoplasma hominis and ureaplasma urealyticum in patients with sterility of tubal origin].the peritoneal fluid of two groups of patients was cultivated; the first one was of 67 patients with tuboperitoneal problem; the second of 14 patients was the control; we founded mollicutes in 39% of the patients of the problem group and 28% of the control group. the correlation between laparoscopic aspects with the positive mollicutes cultures suggested the infection by mollicutes in the tubal cause of sterility.19911765307
emergency cervical cerclage.to assess the efficacy of emergency cervical cerclage.19911777455
an indirect immunofluorescence method for detection of mycoplasma hominis in vaginal smears.we developed a novel method for the detection of mycoplasma hominis from vaginal swabs using an indirect immunofluorescence technique. it is a rapid and simple method that can be finished in only 5 hr and is more sensitive than the usual culture isolation method. the indirect immunofluorescence method was applied to vaginal smears from 193 healthy women and 33.7% gave a positive test. this value was much higher than that (11.4%) obtained from the same specimens by the culture method. when vagina ...19911779888
[preliminary serological survey on mycoplasma and chlamydia infection among gonorrhoea cases].the antibodies to mycoplasma hominis (mh) and chlamydia trachomatis (ct) in 123 sera from the patients with gonorrhoea and 108 sera from the healthy persons control were detected with micro-indirect hemagglutination test. the results showed that the antibody to mh positive rate was 23.58%, gmt 1: 7.60, and to ct 72.35%, gmt 1: 8.71 in the patients. the differences of the antibody levels between the patients and the controls were significant. it was suggested that the urogenital infections caused ...19911782655
induced abortion: microbiological screening and medical complications.preoperative cervical screening of 1,193 women undergoing first-trimester induced abortions yielded chlamydia trachomatis in 11.7%, neisseria gonorrhoeae in 0.8%, mycoplasma hominis in 22.1%, ureaplasma urealyticum in 10.1%, herpes simplex virus in 0.9% and group b streptococci (gbs) in 2.9%. c. trachomatis and n. gonorrhoeae were especially frequent among teenagers. a total of 2.2% (26 women) developed postoperative pelvic inflammatory disease (pid) and 0.9% (13 women) endometritis. pid develop ...19911800368
[age and sex distribution of sexually transmitted diseases in valladolid. a study of 5076 cases].the age and sex distribution of 5,076 cases of s.t.d. dealt with between 1982 and 1988 at the dermatological dispensary of the territorial social welfare service in valladolid are studied. the maximum frequency of s.t.d. (24.1% of cases) corresponded to the age group between 21 and 25 years, which was also the period for maximum prevalence of neisseria gonorrhoeae (26.3%), gardnerella vaginalis (30.7%), candida albicans (27.4%) and acuminata condyloma (33.4%). syphilis (19.9%), chlamydia trachom ...19911801185
[genital mycoplasma colonization in the female genital tract].cervical or vaginal specimens obtained from 1,178 females at different physiologic periods of life were cultured for ureaplasma urealyticum and mycoplasma hominis. low recovery rates of u. urealyticum were obtained in the neonates (20/72, 27.8%) and puerperal (22/83, 26.5%) and postmenopausal (21/70, 30.0%) women. the highest incidence (114/157, 72.6%) was observed in pregnant women. it is suggested that the colonization of genital mycoplasma is associated with the hormonal status of the women.19911804612
recovery of mycoplasma hominis from blood culture media. 19911811128
prostaglandin production by amnion and decidual cells in response to bacterial products.media from bacterial cultures have been tested for actions on prostaglandin biosynthesis by human amnion and decidual cells. the bacterial species, which are commonly associated with intrauterine infections, were group b streptococcus, escherichia coli, fusobacterium nucleatum, bacteroides fragilis, gardnerella vaginalis, neisseria gonorrhea, mycoplasma hominis and ureaplasma urealyticum. overall, low doses of bacterial products were stimulatory of amnion prostaglandin production, whereas high d ...19911857723
[evaluation of the mycoscreen technique for the detection and quantification of genital mycoplasmas].a total of 161 samples from genital sources were evaluated using two different methods for genital mycoplasma isolation: inoculation to urea broth, arginine broth and a7 solid media (standard method) and a new enzymatic method (mycoscreen oxoid). the isolation rates for ureaplasma urealitycum were 42% (standard method) and 49% (mycoscreen) (sensitivity: 100%; specificity: 88%). the isolation rates for mycoplasma hominis were 13% (standard method) and 14% (mycoscreen) (sensitivity: 80%; specifici ...19911863619
newly documented antimicrobial activity of quinolones.the improved antimicrobial activity of newer fluoroquinolones and novel applications recently found for the drugs already marketed are reviewed. several new compounds are more active against gram-positive bacteria than the presently marketed fluoroquinolones. win 57273, the most potent compound in vitro on a weight basis, is 16 to 128 times more active than ciprofloxacin against various staphylococci, streptococci, enterococcus spp., corynebacterium spp., listeria monocytogenes and bacillus spp. ...19911864284
sequence and expression in escherichia coli of a mycoplasma hominis gene encoding elongation factor tu.we describe the molecular cloning and the complete nucleotide (nt) sequence of a mycoplasma hominis gene common to a broad range of mycoplasma species, as defined by hybridization analysis with the cloned gene. production of m. hominis protein in escherichia coli was assayed by use of a monoclonal antibody. the nt sequence analysis revealed a 1194-bp open reading frame that could encode a 43 516-da protein. computer-aided sequence comparison indicated that the gene codes for elongation factor tu ...19911864502
perinatal mycoplasmal infections.maternal infections are known to play a major role in perinatal morbidity and mortality. ureaplasma urealyticum and mycoplasma hominis are sexually transmissible microorganisms associated with a number of pathologic conditions in the pregnant woman including chorioamnionitis and postpartum fever. they are rapidly gaining recognition as significant causes of perinatal infection, especially in infants born prematurely. this review summarizes current knowledge regarding the epidemiology, pathology, ...19911879107
successive synovial mycoplasma hominis isolates exhibit apparent antigenic variation.the expression of surface proteins by 14 successive mycoplasma hominis isolates obtained from the synovial fluid of a chronically infected septic arthritis patient was examined. marked differences in the expression of surface proteins, as determined by monoclonal antibodies raised against the first isolate, were observed. however, identical restriction patterns and virtually identical hybridization patterns with probes containing the conserved genes of the mycoplasma capricolum rrna operon and t ...19911879948
[a case of postpartum fever caused by mycoplasma hominis infection].a 26-year-old housewife was admitted to our hospital with a history of high fever after previous cesarean delivery. she had premature rupture of the membrane on the 41st week of pregnancy and the amniotic fluid was found to be cloudy on the fourth day after rupture. therefore, cesarean delivery was performed. on the first day of operation, her body temperature increased up to 38 degrees c in spite of the treatment with latamoxef (lmox), 3 g/day. a sample of intrauterine material yielded m. homin ...19911880452
gonorrhea in women prostitutes: clinical data and auxotypes, serovars, plasmid contents of ppng, and susceptibility profiles.eighty-nine women prostitutes who underwent clinical and microbiologic examination were found to have gonococcal infection. the median age was 22; 92.1% were from urban areas. nearly all the women prostitutes refrained from barrier methods (92.1%) and had contact with several partners (91.0%). the most frequent clinical findings were leukorrhea (50.6%), cervicitis (20.2%), and pelvic inflammatory disease (pid) (18.0%). eighty-one women prostitutes (93.1%) had experienced a previous std, with chl ...19911902990
[mycoplasmas in the vaginal flora in colpitis].the vaginal secretion of 194 women was subjected to bacteriological examination. the results of cultivation examinations were compared with mop examinations. the widest bacterial spectrum was recorded in mop ii. no differences were recorded as regards detection of lactobacilli and potentially pathogenic bacteria in mop i, ii and iii. the percentage of women with ureaplasma urealyticum was equal in case of mop i, ii and iii. mycoplasma hominis was isolated significantly more frequently in mop ii ...19911913859
microbiological, epidemiological and clinical correlates of vaginal colonisation by mobiluncus species.the microbiological and epidemiological correlates of vaginal colonisation by mobiluncus species were examined among randomly selected women attending a sexually transmitted disease (std) clinic. women positive for trichomonas vaginalis were excluded. mobiluncus spp. were detected by gram stained vaginal smear in 21% of 633 std clinic patients, including 53% of those with and 4% of those without bacterial vaginosis (bv), as diagnosed by clinical criteria. gardnerella vaginalis and mycoplasma hom ...19911916772
in vitro susceptibilities of mycoplasma pneumoniae, mycoplasma hominis, and ureaplasma urealyticum to sparfloxacin and pd 127391.the in vitro activities of two investigational quinolones, sparfloxacin (previously designated at 4140) and pd 127391, were determined for 30 strains each of mycoplasma pneumoniae, mycoplasma hominis, and ureaplasma urealyticum and compared with those of ciprofloxacin, tetracycline, clindamycin, and erythromycin. erythromycin was the most active compound against m. pneumoniae (maximum mic, less than 0.008 microgram/ml). pd 127391 (mics, less than 0.008 to 0.031 microgram/ml), sparfloxacin (mics, ...19911929260
susceptibilities of mycoplasma hominis and ureaplasma urealyticum to two new quinolones, sparfloxacin and win 57273.mycoplasma hominis was highly susceptible to two new quinolones, with mics for 90% of isolates tested of 0.004 micrograms/ml for win 57273 and 0.063 micrograms/ml for sparfloxacin, which were activities much greater than the 1 microgram/ml found for ofloxacin and tetracycline. although ureaplasma urealyticum was less susceptible, the mics for 90% of isolates tested of 0.25 micrograms/ml for win 57273 and 0.5 micrograms/ml for sparfloxacin were four- to eightfold greater than those found for oflo ...19911929320
recovery of mycoplasma hominis from blood culture media.although several workers have demonstrated that mycoplasma hominis is responsible for 2 to 20% of cases of septicaemia, reports from britain do not support this premise--possibly because many of the blood culture systems used fail to sustain growth of this organism. previous work suggests that this is probably due to the inhibitory effects of sodium polyanethol sulphonate (liquoid) on m. hominis. in an extension of an in-vitro evaluation of blood culture systems, gelatin was shown to give some p ...19911943535
the effect of ciprofloxacin and doxycycline plus metronidazole on lower genital tract flora in patients with proven pelvic inflammatory disease.alterations in the lower genital tract flora were studied during antimicrobial treatment of 36 women with acute pelvic inflammatory disease (pid). sixteen women received ciprofloxacin, and 20 received doxycycline plus metronidazole. both regimes eradicated chlamydia trachomatis in all cases and neisseria gonorrhoeae in all except one in which combination treatment failed because of resistance of the organism to tetracycline. both regimes eradicated mycoplasma hominis in all cases, while ciproflo ...19911953057
genital mycoplasmas in preterm infants: prevalence and clinical significance.the genital mycoplasmas: ureaplasma urealyticum and mycoplasma hominis have recently assumed an increasing importance as neonatal pathogens. the aim of the present survey was to determine the prevalence of infections with these organisms in preterm infants in two neonatal intensive care units in israel. among 99 preterm infants, 24 (24%) harboured mycoplasmas in their throats shortly after birth. u. urealyticum was the most common organism. m. hominis was isolated only from 3 infants. six out of ...19911959546
comparative in vitro activity of azithromycin, clarithromycin, erythromycin and lomefloxacin against mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum.the in vitro activity of three macrolides, azithromycin, clarithromycin and erythromycin and a new fluoroquinolone, lomefloxacin, against pathogenic mycoplasma (16 to 18 strains of mycoplasma pneumoniae, 41 to 77 strains of mycoplasma hominis, 65 to 104 strains of ureaplasma urealyticum) was compared. the three macrolides were highly active against mycoplasma pneumoniae. clarithromycin was the most active macrolide against ureaplasma urealyticum whereas azithromycin was somewhat more active than ...19901964899
low incidence of positive amnionic fluid cultures in preterm labor at 27-32 weeks in the absence of clinical evidence of chorioamnionitis.in order to determine the utility of amniocentesis for detecting subclinical chorioamnionitis in asymptomatic afebrile women in preterm labor with intact membranes, we enrolled 47 women between 27-32 weeks' gestation in a prospective study. after enrollment, 38 women fulfilled all clinical and laboratory criteria for the study; nine women were excluded because they had a leukocyte count exceeding 15,000/microl. none of the 38 asymptomatic afebrile women had a positive culture from the amnionic f ...19911988886
the epidemiology of group b streptococcal colonization in pregnancy. vaginal infections and prematurity study group.risk factors for cervicovaginal group b streptococcal colonization at 23-26 weeks' gestation were studied in 7742 women participating in the vaginal infections and prematurity study. the prevalence of group b streptococcus was 18.6%, and was greatest in (predominantly caribbean) hispanics from new york city, followed by blacks, whites, and other (predominantly mexican) hispanics. group b streptococcus was more common among older women and women of lower parity, and less common among women living ...19912002986
antepartum cultures for ureaplasma urealyticum are not useful in predicting pregnancy outcome. the vaginal infections and prematurity study group.to test the hypothesis that genital colonization with ureaplasma urealyticum would predict adverse pregnancy outcome, 4934 women from five medical centers were evaluated for vaginal colonization with u. urealyticum between 23 and 26 weeks' gestation and followed up to delivery. u. urealyticum colonization was associated with maternal age, parity, racial-ethnic group, martial status, income, education, smoking, number of sexual partners, and colonization with trichomonas vaginalis, mycoplasma hom ...19912003532
use of the mycoplasma hominis 102-116 kd proteins as antigen in an enzyme-linked immunosorbent assay.mycoplasma hominis surface structures involved in human immune response and in the pathogenesis of this bacterial infection are inadequately defined. attempts have been made to identify m. hominis surface proteins, to determine the antigenicity of these polypeptides, and to examine antigens which could lead to the development of species-specific diagnostic tests. by means of western blotting, using a pool of sera from patients with culturally proven vaginal infection, most antigens recognized we ...19912014008
chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum infections in women. prevalence, risks and management at a south african infertility clinic.chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. routine screening tests or empirical antibiotic treatment of infertile couples may be justified by the prevalence of these organisms. we studied the wives in 40 consecutive infertile couples. monoclonal direct immunofluorescence (dif) for c trachomatis was performed on fixed smears from endocervical swabs. m homini ...19912030488
prevalence of sexually transmitted pathogens among women attending a methadone clinic in israel.to assess the prevalence of sexually transmitted pathogens in drug-addicted women in tel aviv, israel.19912032707
mycoplasma hominis wound infection following aortobifemoral bypass. 19912037094
identification of a mycoplasmal protein which binds immunoglobulins nonimmunologically.immunoblotted protein samples from several strains of mycoplasma hominis and from one strain of mycoplasma arginini each contain a polypeptide of a molecular mass of 95,000 to 105,000 da which binds immunoglobulin nonimmunologically. immunoblots from these organisms were probed with alkaline phosphatase-conjugated goat anti-rabbit immunoglobulin, conjugated goat immunoglobulin g (igg) fab fragments, and conjugated goat igg fc fragments. the polypeptide bound the goat anti-rabbit molecules and th ...19912037376
isolation of mycoplasma hominis from peritoneal effluent of a capd patient. 19912049432
peritonitis due to mycoplasma hominis and ureaplasma urealyticum in a liver transplant recipient. 19912060519
analysis of the nucleotide sequence of the mycoplasma hominis tuf gene and its flanking region.a gene from mycoplasma hominis pg21 similar to the tuf gene encoding the elongation factor tu (ef-tu) of escherichia coli was cloned and sequenced. the 1193-bp open reading frame flanked by a putative promoter and a potential stem-and-loop structure encoded a 44-kda polypeptide. the tuf gene of m. hominis pg21 has the lowest g + c content seen in prokaryotes (38.2%). a gene (mhlmp1) encoding a variable surface exposed membrane protein (lmp1) was found downstream the 3' end of the tuf gene. it wa ...19912060757
[value and limitations of the research of mycoplasma hominis and ureaplasma urealyticum in gastric fluid in newborn infants].mycoplasma hominis and ureaplasma urealyticum were cultured and counted in the gastric fluid of 153 neonates divided into three groups: 28 preterm neonates managed in an intensive care unit (group i); 83 full term neonates with suspected infection (group ii); and 42 full term neonates with not evidence of infection (group iii). the colonization rate (17.85%) in the intensive care unit group was not significantly different from the rates seem in the two other groups. these results do not militate ...19912062554
[infections by mycoplasma hominis and ureaplasma urealytycum in vaginal discharge. incidence and treatment].this report summarizes the studies carried out on 100 patients who were examined for vaginal discharge. the following observations were made: mycoplasma hominis in 13.59% of the cases, ureaplasma urealytycum in 8.73% of the cases, that is, the presence of at least one of these microorganisms in 22.32 of the cases. all these infections were treated by tetracyclines administered in appropriate doses.19912068483
mycoplasma hominis infection in three renal transplant patients.we describe three cases of extragenital infection by mycoplasma hominis in three patients transplanted with kidneys from cadaver donors. in two patients, the microorganism was isolated in the exudate from the surgical wound after 72 hrs. of culture on blood-agar (columbia + 5% horse blood) in co2 and under anaerobic conditions. in the remaining case, m. hominis was isolated in urine from a suprapubic catheter. all three patients responded satisfactorily to treatment with doxycycline. mycoplasma ...19902076594
evaluation of the significance of mycoplasma hominis and ureaplasma urealyticum in female genital tract infection--a retrospective case note study.routine screening for sexually transmitted diseases in new patients attending the genitourinary clinic in stoke-on-trent includes a culture for mycoplasma hominis (mh) and ureaplasma urealyticum (uu). a retrospective study was carried out on 400 female patients to ascertain whether there were any significant differences between the group positive for mh and uu and the negative control group. the positive group were found to be younger on average, but to have similar sexual histories to the negat ...19902083293
increase in resistance of mycoplasma hominis to tetracyclines.isolates of mycoplasma hominis collected from patients in boston and new york between 1976 and 1989 were studied. minimal metabolism-inhibiting concentrations (mmcs) were determined by use of a terminal color change-broth dilution method, as well as by an agar inoculum-broth dilution method. both methods gave comparable results. tetracycline mmcs for 3 (7.0%) of 43 isolates of m. hominis collected from 1976 to 1979 were 8 micrograms/ml or more, but they were 8 micrograms/ml or more for 11 (20.3% ...19902088184
mycoplasmas, a review of surveys examining human genital infections and experimental infection in mice with special reference to in vitro fertilization.patients attending an "in vitro fertilization" clinic were shown to be commonly infected in the semen with ureaplasma urealyticum (29%) and mycoplasma hominis (12%). other mycoplasmas were rarely recovered. m. hominis was not eradicated from sperm by washing, although washing removed 71% of the ureaplasmas from infected semen. there was a significantly higher incidence of u. urealyticum and m. hominis in patients with cervical intraepithelial neoplasia (p = less than 0.05 and p = less than 0.01 ...19902097465
serologic evidence for the role of chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma hominis in the etiology of tubal factor infertility and ectopic pregnancy.the authors used enzyme immunoassay to determine the prevalence of serum antibodies to the sexually transmitted disease (std) organisms chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma hominis among 104 infertile women undergoing in vitro fertilization. altogether, 55 (72%) out of 76 women with tubal abnormalities tested positive for one or more std organisms, compared with only 6 (21%) out of 28 infertile women with normal tubes (p less than .001). the authors obtained positive test ...19902106171
pefloxacin compared with cefotaxime for treating men with uncomplicated gonococcal urethritis.in a randomized comparative study, 83 male patients suffering from acute uncomplicated gonococcal urethritis were treated with a single dose of either 0.8 g pefloxacin, given orally, or 1.0 g cefotaxime, given intramuscularly. the cure rates were 100% in both treatment groups four to seven days and 21 to 31 days, respectively, after therapy. the mics of the isolated neisseria gonorrhoeae ranged from 0.008 to 0.06 mg/l for pefloxacin and from 0.0005 to 0.03 mg/l for cefotaxime. postgonococcal ure ...19902124210
characterization of the tet m determinants in urogenital and respiratory bacteria.tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon.19902159259
postgonococcal urethritis: a double-blind study of doxycycline vs placebo.the occurrence of postgonococcal urethritis (pgu) was studied in heterosexual men who were randomly assigned to receive either oral doxycycline 100 mg or a placebo capsule twice daily for seven days in addition to routine single dose treatment of urethral gonorrhoea. of 216 patients suitable for assessment, pgu was diagnosed in 54/104 (51.9%) in the placebo group and 30/112 (26.8%) in the doxycycline group. symptomatic pgu was uncommon (6.7% in the placebo group, 2.7% in the doxycycline group). ...19902179113
etiology and treatment of post-cesarean-section endometritis after cephalosporin prophylaxis.to describe the microbiologic etiology of post-cesarean endometritis developing after perioperative cephalosporin prophylaxis, endometrial samples were obtained from 27 women with a triple-lumen catheter. the women were assigned in a double-blind, randomized fashion to receive either ticarcillin/clavulanate, 3.1 g, or cefoxitin, 2 g, administered every six hours, until the clinical signs of infection resolved. a total of 149 microorganisms (84 facultative and 65 obligate anaerobes) were recovere ...19902181119
[role of mycoplasma hominis in acute infections of the female genital system].a study was carried out on the role of mycoplasma hominis in obstetric and gynecological infections, and the paper reports a case report of acute pelvoperitonitis in a woman fitted with an iud. the important role of early preoperative diagnosis in underlined and the current criteria for correct antibiotic therapy are discussed.19902187167
[evaluation of the mycoplasma plus and the sir mycoplasma kits for quantitative detection and antibiotic susceptibility testing of genital mycoplasma].we compared the results obtained with two commercially available systems (diagnostics pasteur) for the quantitative identification and the antibiotic susceptibility testing of the genital mycoplasmas. ureaplasma urealyticum and mycoplasma hominis with established methodologies, i.e. isolation on agar with enumeration by dilutions in broth medium and mic determinations. the mycoplasma plus system, consisting of six cups, was designed for the identification and quantitation of genital mycoplasmas ...19902195452
[epidemiology of vaginal mycoplasmas].the authors made in 456 women mycoplasmatological examinations of the vaginal secretion by cultivation. mycoplasma hominis was obtained in 158 women (34.6%). u. urealyticum in 295 women (64.7%) in 120 women (26.3%) both microorganisms were present. the prevalence of vaginal mycoplasms is markedly influenced by the sexual activity of women. the relative risk of colonization of the vagina with m. hominis rises with the frequency of sexual intercourse more than four times. in case of a larger numbe ...19902196998
extragenital mycoplasma hominis infections in adults.purpose: to heighten awareness of the role of mycoplasma hominis as an extragenital pathogen in adults. patients and methods and results: patients ranged in age from 14 to 76 years. thirteen patients were immunosuppressed, including nine organ transplant recipients; three were receiving steroids, and two had an underlying malignancy. the remainder were immunocompetent. thirteen patients had prior surgery at or near the site of infection. m. hominis was isolated from normally sterile sites such a ...19902203260
occurrence of ureaplasma urealyticus and mycoplasma hominis in non-gonococcal urethritis before and after treatment in a double-blind trial of ofloxacin versus erythromycin.the efficacy of ofloxacin, a new quinolone derivate, was tested against that of erythromycin in a prospective double-blind trial in patients with non-gonococcal urethritis (ngu) with special reference to the occurrence of mycoplasma hominis and ureaplasma urealyticum. 188 male ngu patients were randomized to treatment with either ofloxacin 200 mg b.i.d. or erythromycin 500 mg b.i.d. for seven days. before treatment eight (4.3%) patients, five in the erythromycin group and three in the ofloxacin ...19902218419
enzyme linked immunosorbent assay for immunoglobulins g to mycoplasma hominis.sera (187) from women patients attending the std, obstetrics and gynaecology, and family planning clinics were screened for the presence of the igg class of immunoglobulins against m. hominis, using the elisa technique. sonicates of locally isolated m. hominis serotype (cs1) and standard pg 21 strain were used as antigens. the test was standardized using penicillinase as an enzyme. the elisa showed 90.48 per cent sensitivity and 84.8 per cent specificity, and was also rapid (as compared to cultu ...19902228057
reservoir of four organisms associated with bacterial vaginosis suggests lack of sexual transmission.this study consisted of a search for the possible reservoir and mode of spread of the four bacterial vaginosis-associated organisms mobiluncus mulieris, mobiluncus curtisii, mycoplasma hominis, and gardnerella vaginalis. their occurrence in rectal, oral, and pharyngeal specimens from women with and without bacterial vaginosis, their male sexual consorts, four homosexual men, and children (altogether, 374 people) was studied. genital samples were also obtained from all adults. all four organisms ...19902229386
antenatal microbiologic and maternal risk factors associated with prematurity.in a prospective study of 202 women (gestational age 24 +/- 4 weeks), we evaluated possible influences of lower genital tract infection or bacterial conditions on obstetric outcomes, including preterm labor, preterm premature rupture of membranes, and preterm birth. the presence of bacterial vaginosis (18.7%) was associated with an increased risk of preterm labor (relative risk, 2.6; 95% confidence interval, 1.08 to 6.46). for women with bacterial vaginosis who also had mobiluncus species morpho ...19902240089
cervicovaginal microflora and pregnancy outcome: results of a double-blind, placebo-controlled trial of erythromycin treatment.available information suggests that some instances of preterm birth or premature rupture of membranes are associated with clinically unrecognized infection and inflammation of the lower uterine segment, decidua, and fetal membranes. various cervicovaginal microorganisms have been recovered from these sites. many of these microorganisms produce factors that may lead to weakening of the fetal membranes, release of prostaglandins, or both. this study evaluated the presence of various lower genital ...19902240110
[microbial flora in semen of infertile african men at garankuwa hospital].this study was undertaken to determine the frequency of bacteria and mycoplasmas in semen of infertile african men and to compare abnormal semen parameters with the presence of these organisms. routine semen analysis were performed on 100 patients, and semen and first-voided urine samples were cultured. mycoplasma hominis was cultured in 28% of seminal fluid and ureaplasma urealyticum in 42%. these organisms were both present in 22% of cultures. bacteriological cultures were positive in 6 urine ...19902264614
mycoplasma hominis infections in female genital tract & use of immunofluorescence for antibody detection.a total of 325 women with genital tract infection and 108 healthy controls were screened for the presence of mycoplasmas. of these, 325 patients, mycoplasmas were recovered in 186. thirty five isolates were m. hominis and 151 isolates of ureaplasma urealyticum. the fluorescent-antibody technique (fat) has been employed for the rapid identification of mycoplasma colonies growing on agar plates. the method was found to be rapid for detection of m. hominis growing on primary isolation plates. the a ...19902269508
differences in accumulation of radiolabeled amino acids and polyamines by mycoplasma and acholeplasma species.four species in the order mycoplasmatales, mycoplasma capricolum, mycoplasma hominis, mycoplasma arginini, and acholeplasma laidlawii, were compared for their ability to accumulate radiolabeled amino acids and polyamines. the use of a novel high-molecular-weight (hmw) medium, from which molecules of less than 12,000 molecular weight had been removed by extensive dialysis, allowed us to discern significant differences among the species in their relative accumulations of [3h]methionine and [3h]leu ...19902275861
wound infections after cesarean section with mycoplasma hominis and ureaplasma urealyticum. a report of three cases.mycoplasma hominis and ureaplasma urealyticum were isolated from the surgical wounds of three patients who developed endometritis and a wound infection after cesarean section. in all patients, aspiration of the incision yielded a cloudy serosanguinous exudate. gram stain of the fluid revealed numerous white blood cells but no bacteria. all patients responded to antibiotic therapy and local wound care.19902282776
common sulfoglycolipid receptor for mycoplasmas involved in animal and human infertility.sulfoglycolipids are ubiquitous components of the male germ cell membrane. sulfogalactoglycerolipid (sgg) is restricted to mammalian cells and has recently been implicated in sperm/egg interactions. mycoplasma infections have been implicated in infertility in a variety of species, including humans. four such species-specific mycoplasmas, ureaplasma urealyticum and mycoplasma hominis (humans), mycoplasma pulmonis (rodents), and ureaplasma diversum (cattle) are not shown to specifically recognize ...19902289018
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