| [transformation of mycoplasma hominis by plasmid pam120 using electroporation]. | mycoplasma hominis was transformed by electroporation with plasmid pam120 containing the transposon tn916 that carried the tetm gene responsible for the resistance to tetracycline. the frequency of transformation was 10(-7)-10(-8) colony-forming units (cfu) per 10 micrograms of plasmid dna. the pcr analysis of transformed dna confirmed the transposon integration into the mycoplasma genome. | 2000 | 10779903 |
| [subdural empyema due to mycoplasma hominis following epidural anesthesia]. | in the literature there are sporadic reports of spinal epidural abscesses after epidural anaesthesia (staphylococcus aureus in 82%), whilst subdural empyemas are more often related to ear and sinus conditions. | 2000 | 10789141 |
| in vitro activity of a cellulose acetate phthalate topical cream against organisms associated with bacterial vaginosis. | | 2000 | 10797102 |
| association of ureaplasma urealyticum with abnormal reactive oxygen species levels and absence of leukocytospermia. | ureaplasma urealyticum is a commensal of the lower genitourinary tract of many sexually active adults. the organism is more common in partners of infertile than fertile marriages. we conducted a prospective study at our tertiary care center to confirm a possible association between u. urealyticum and abnormal sperm function parameters. | 2000 | 10799180 |
| [mixed urogenital infection in men]. | high occurrence of sexually transmitted diseases (std) caused by a combination of different infective agents was established. in 62.5% of cases such diseases took a course typical for mixed infections. combinations of infective agents of std were found to have several typical associations, e.g. ureaplasma urealyticum and mycoplasma hominis in combination with opportunistic microflora. the microbiocenosis of the reproductive tract was shown to form under the influence of a complex of endogenous ( ... | 2000 | 10808589 |
| comparative potency of gemifloxacin, new quinolones, macrolides, tetracycline and clindamycin against mycoplasma spp. | this study determined the comparative in vitro potency of the new investigative quinolone gemifloxacin (sb-265805) using low-passaged clinical isolates and type strains of mycoplasma commonly found in the human respiratory and urogenital tracts. organisms studied were mycoplasma pneumoniae, mycoplasma hominis, mycoplasma fermentans, mycoplasma genitalium, mycoplasma penetrans and ureaplasma urealyticum, obtained from different geographical regions. comparator drugs were levofloxacin, trovafloxac ... | 2000 | 10824029 |
| updated phylogenetic description of the mycoplasma hominis cluster (weisburg et al. 1989) based on 16s rdna sequences. | the fastidious nature of the mollicutes (mycoplasmas), their lack of a classic bacterial cell wall, and their very small genome, make phylogenetic placements of new species in this enlarging group of prokaryotes an important and valuable aid in their classification. in this report we have determined the phylogeny of the mycoplasma hominis cluster of the hominis group. the 16s rdna sequences from several previously described mycoplasma species were determined and ten species were found to belong ... | 2000 | 10826816 |
| observations on the antibiotic treatment of experimentally induced mycoplasmal infections in mice. | antibiotics were given subcutaneously to female mice, colonized by mycoplasmas in the vagina and by others in the oropharynx. mycoplasma pulmonis was eradicated from the vagina of 36 of 42 immunocompetent to or balb/c mice with oxytetracycline, and from 17 of 18 to mice with lymecycline. mycoplasma hominis was eradicated from the vagina of all of 18 immunocompetent balb/c mice with oxytetracycline or tetracycline. regarding oropharyngeal organisms, m. pulmonis was eradicated from only 20 of 42 t ... | 2000 | 10837449 |
| host and tissue specificity of trichomonas vaginalis is not mediated by its known adhesion proteins. | adhesion of trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. however, the specific receptors on the host cell have not yet been identified. in this work, the ability of the t. vaginalis adhesins to bind to cells of different histologic derivations and from different species has been studied. hela, cho, and vero cell lines; erythrocytes from different ... | 2000 | 10858260 |
| comparative activities of telithromycin (hmr 3647), levofloxacin, and other antimicrobial agents against human mycoplasmas. | the activities of telithromycin and levofloxacin against 99 mycoplasma strains were compared to those of several macrolides, ofloxacin, and doxycycline. telithromycin mics of </=0.25 microgram/ml were found for all isolates, except for mycoplasma hominis, while levofloxacin was active at concentrations of </=1 microgram/ml against all species studied. | 2000 | 10858366 |
| mycoplasmal antibodies as determined with an enzyme-linked immunosorbent assay, in tubal factor infertility. | a total of 81 infertile women, who had been referred for diagnostic loparoscopy, were tested for the presence of antibodies to mycoplasma hominis and t-mycoplasma. out of 81, 30 had tubal adhesions and 51 had unilateral/bilateral tubal blockage. antibodies to m. hominis were found in 21/30 (70%) and 14/51 (27.45%) women, antibodies to t-mycoplasma in 12/20 (40% and 39/51 (76.47%) women with tubal disorder. in a control group of 40 pregnant women, antibodies to the same two organisms occurred in ... | 1999 | 10862270 |
| single dose oral azithromycin versus seven day doxycycline in the treatment of non-gonococcal mucopurulent endocervicitis. | the aim of this study was to compare single dose oral azithromycin versus seven-day doxycycline in the treatment of non-gonococcal mucopurulent cervicitis (mpc). one hundred and thirty-one women with non-gonococcal mpc were enrolled in a prospective-randomised study to compare the efficacy and safety of a single oral dose of 1 g azithromycin and a seven-day course of 100 mg doxycycline twice daily. clinical examination and culture samples for chlamydia trachomatis and other microorganisms were p ... | 2000 | 10870778 |
| the association of mycoplasma hominis, ureaplasma urealyticum and mycoplasma genitalium with bacterial vaginosis: observations on heterosexual women and their male partners. | the prevalence of 3 mycoplasmas (mycoplasma hominis, ureaplasma urealyticum and mycoplasma genitalium) was determined in a cohort of women with or without bacterial vaginosis (bv) and in their respective male partners. heterosexual women with or without bv and their male partners were recruited and genital sampling for these microorganisms was performed. seventeen women with bv and 21 women with normal flora, and their respective male partners, were recruited. m. hominis was present in 9 (53%) o ... | 2000 | 10872907 |
| the prevalence of urethral infections amongst asymptomatic young men in hat yai, southern thailand. | the aim of this study was to survey sexual behaviour and estimate the prevalence of urethral infections amongst male vocational college students. a cross-sectional survey was performed among 479 young men attending 2 vocational colleges in hat yai, southern thailand. polymerase chain reaction (pcr) tests of first-void urine (fvu) samples were used to detect infection with chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum, mycoplasma genitalium and mycoplasma hominis. girlfrien ... | 2000 | 10872914 |
| [neonatal infections with genital mycoplasmas]. | | 2000 | 10904752 |
| [biological diagnosis of infections caused by mycoplasmas]. | | 2000 | 10904755 |
| [bacterial vaginosis. epidemiology and risk factors]. | bacterial vaginosis (bv) is a change in vaginal ecosystem where lactobacilli dominate, flora is absent or greatly reduced, and replaced with a mixed, predominantly anaerobic flora, consisting of gardnerella vaginalis, mycoplasma hominis, mobiluncus spp, bacteroides spp, prevotela spp, peptostreptococcus spp, fusobacterium spp and porphyromonas spp. the concentration of bacteria increases from 100 to 1000 fold in women with bv compared to healthy women. bv has been formerly known as nonspecific v ... | 2000 | 10916461 |
| symptomatic bacteriuria due to ureaplasma and mycoplasma in adults. | 314 women, and 52 men were screened during 18 months, for the presence of ureaplasma urealyticum and mycoplasma hominis in a midstream specimens of urine. all the patients were adults with symptoms of urinary tract infection who consulted a physician at a primary health center "josé castro villagrana" in tlalpan, d.f. méxico. ureaplasma urealyticum was isolated in 54 (17.1%) of the women and 8 (15.4%) of the men. only escherichia coli and gardnerella vaginalis were isolated more frequently in bo ... | 1998 | 10932729 |
| bacterial vaginosis a "broad overview". | bacterial vaginosis (bv) was first reported in 1995 by gardner and dukes, who described the unique clinical signs and symptoms and the distinctive nature of the vaginal discharge associated with it. they also described a "new" causative organism, which they named "haemophilus vaginalis", subsequently renamed gardnerella vaginalis. bv is currently the most prevalent cause of infectious vaginitis among women attending for genitourinary diseases. bv has a complex microbiology. lactobacillus populat ... | 1999 | 10932748 |
| cytokine concentrations in seminal plasma from subfertile men are not indicative of the presence of ureaplasma urealyticum or mycoplasma hominis in the lower genital tract. | the inflammatory response to the presence of ureaplasma urealyticum or mycoplasma hominis in the lower genital tract of subfertile men without any signs or symptoms of infection was investigated by measuring the concentrations of interleukin (il)-6, il-8, tumour necrosis factor-alpha (tnf-alpha) and interferon-gamma (ifn-gamma) in seminal plasma. semen samples were collected from 30 culture-positive subfertile males and 23 culture-negative subfertile males. enzyme-linked immunosorbent assays sho ... | 2000 | 10933253 |
| relationship of bacterial vaginosis and mycoplasmas to the risk of spontaneous abortion. | this study was undertaken to investigate a possible link between first-trimester diagnosis of bacterial vaginosis and cessation of pregnancy at < or =20 weeks' gestation. | 2000 | 10942482 |
| association of mycoplasma hominis and ureaplasma urealyticum with some indicators of nonspecific vaginitis. | the purpose of this study was to determine the isolation rates of mycoplasma hominis and ureaplasma urealyticum from three populations of women and also to relate the presence of these microorganisms with some indicators of nonspecific vaginitis. three hundred vaginal swabs were taken from delivery, pregnant and control (not pregnant) women. cultures were done in e broth supplemented with arginine or urea. m. hominis was isolated in 5% at delivery, 12% from pregnant and 5% from control women and ... | 2000 | 10948822 |
| in vitro activity of trovafloxacin compared to those of five antimicrobials against mycoplasmas including mycoplasma hominis and ureaplasma urealyticum fluoroquinolone-resistant isolates that have been genetically characterized. | the in vitro activity of trovafloxacin against 125 strains of mycoplasma species and ureaplasma urealyticum, including fluoroquinolone-susceptible and fluoroquinolone-resistant species, was compared to those of other fluoroquinolones, doxycycline, and erythromycin. the mic at which 90% of isolates are inhibited for all fluoroquinolone-susceptible strains was 0.25 microg/ml. whatever the associated mutations, trovafloxacin exhibited greater activity than the other fluoroquinolones tested against ... | 2000 | 10952617 |
| prevalence and antibiotic susceptibility of ureaplasma urealyticum in malaysian neonates with respiratory distress. | ureaplasma urealyticum was isolated from the endotracheal aspirates of 39 (21.4%) of 182 neonates with respiratory distress requiring ventilatory support. mycoplasma hominis was isolated from one (0.5%) neonate. bacterial cultures were negative in 123 (67.6%) neonates. antibiotic susceptibility test carried out on ten isolates of u. urealyticum showed that all the organisms were sensitive to erythromycin but resistant to lincomycin and sulfamethoxazole trimethoprim. all, except one, u. urealytic ... | 1997 | 10968119 |
| effect of intravaginal clindamycin cream on pregnancy outcome and on abnormal vaginal microbial flora of pregnant women. | to determine whether intravaginal clindamycin cream reduces the incidence of abnormal pregnancy outcome in women with abnormal vaginal microbial flora graded as intermediate or bv and to investigate the effect of the antibiotic on vaginal microbial flora. | 2000 | 10968599 |
| relevant prevalence of mycoplasma hominis and ureaplasma urealyticum serogroups in hiv-1 infected men without urethritis symptoms. | m. hominis and u. urealyticum are the better-known mycoplasma species pathogenic to the human genitourinary tract, causing mainly urethritis, bacterial vaginosis and pregnancy complications. in hiv-infected patients, the prevalence and role of these species is still not well known. the aim of this work was to determinate the prevalence of these species in this group of male patients (hiv group), in comparison to a group of men with clinical symptoms of urethritis (std group). m. hominis was isol ... | 2000 | 10968880 |
| colonization and transmission of ureaplasma urealyticum and mycoplasma hominis from mothers to full and preterm babies by normal vaginal delivery. | a prospective study was carried out among pregnant women and their newborn babies in the university hospital, kuala lumpur from january 1996 to june 1997. the maternal cervical colonization rates of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) were found to be 57.5% and 15.8% respectively while the isolation rates from nasopharyngeal secretions of the newborns were 50.8% for uu and 6.6% for mh. the overall transmission rates were 88.4% for uu and 42.1% for mh. there was no significant ... | 1999 | 10972036 |
| association of ureaplasma urealyticum colonization in male urethra and condyloma acuminatum. | the frequent coexistence of two or more sexually transmitted diseases in one patient has been reported in non-dermatological literature, mostly in languages other than english. identification of ureaplasma urealyticum, chlamydia trachomatis and mycoplasma hominis in men with other stds is important, since these bacteria have been implicated in a variety of diseases such as non-gonococcal urethritis, premature rupture of fetal membranes, and infertility in female sexual partners of these patients ... | 2000 | 10979348 |
| characterization of the variability of a 75-kda membrane protein in mycoplasma hominis. | the gene p75 encoding a 75-kda surface-exposed membrane protein p75 was cloned and sequenced from mycoplasma hominis type strain pg21t. to investigate the intraspecies variability, sequences were obtained from an additional two isolates 7488 and 183, and the three sequences were compared. the nucleotide and amino acid differences were not confined to specific regions of the gene/protein, but when comparing the three sequences, differences were present as single site substitutions or small insert ... | 2000 | 10981709 |
| cloning and nucleotide sequence of the dna gyrase (gyra) gene from mycoplasma hominis and characterization of quinolone-resistant mutants selected in vitro with trovafloxacin. | we report the cloning and characterization of the gyra gene of the mycoplasma hominis dna gyrase, which was previously shown to be associated with quinolone resistance in this organism. the 2,733-bp gyra gene encodes a protein of 911 amino acids with a calculated molecular mass of 102.5 kda. as expected, m. hominis gyra exhibits higher homology with the gyra subunits of the gram-positive bacteria clostridium acetobutylicum, bacillus subtilis, streptococcus pneumoniae, and staphylococcus aureus t ... | 2000 | 10991851 |
| genital mycoplasmas among pregnant women in cóte d'ivoire, west africa: prevalence and risk factors. | a cross-sectional study was conducted among pregnant women in côte d'ivoire to assess the prevalence and the factors associated with mycoplasma colonization. a positive culture was found in 400 of 551 women (73%); ureaplasma urealyticum was identified in 22%, mycoplasma hominis in 20% and both microorganisms in 31%. mycoplasma colonization was not associated with genital symptoms or signs. young age, low educational level, antimicrobial chemotherapy before consultation and presence of bacterial ... | 2000 | 10997504 |
| role of the male partner in the lower genitourinary tract infection of female. | we studied the relationships of selected microbes and the role of consorts in the causation of vaginal discharge which may be due to cervicitis or vaginitis. a total of 93 consecutive patients in the reproductive age group with symptoms of vaginal discharge along with their sexual partners were studied. samples were collected from the cervix and posterior fornix of the female patients and from the urethra and sub-prepucial area of the male partner for culture of neisseria gonorrhoeae, gardnerell ... | 2000 | 11006655 |
| prosthetic valve endocarditis caused by mycoplasma hominis. | mycoplasma hominis endocarditis is extremely uncommon and difficult to diagnose. atypical growth characteristics in routine bacterial culture and an inability to demonstrate the organism using gram staining can lead to a delayed diagnosis of mycoplasma hominis infections, and the organism is often missed. this report describes a patient with mycoplasma hominis prosthetic valve endocarditis. the microorganism was recovered from the mitral prosthesis but was missed in blood cultures. this finding ... | 2000 | 11014630 |
| [laboratory diagnosis of mycoplasma infections in man]. | | 2000 | 11031436 |
| [the effect of velikobagachanskaia mineral water on the course of chronic pyelonephritis]. | the employing of velikobagachanskaia mineral water internally by drinking and externally by taking a bath with it adds to the effectiveness of treatment of chronic pyelonephritis, modulates parameters associated with immune homeostasis, reduces the number of carriers of antibodies to m. hominis, facilitates prolongation of clinical and laboratory remission. | 2000 | 11031464 |
| distribution of ecto 5'-nucleotidase on mycoplasma species associated with arthritis. | the enzyme ecto 5'-nucleotidase (5'n) was found to be active on 8/14 strains of mycoplasma fermentans, k(m) (+/-s.d.) 3.8+/-2.8 microm 5'-amp, and on the type strain of mycoplasma pulmonis, k(m) 0.63 microm 5'-amp. the six m. fermentans strains lacking 5'n activity were related by restriction fragment length polymorphism typing. at ph 8.5, the type strains of mycoplasma arthritidis, mycoplasma buccale and ureaplasma urealyticum showed a relatively non-specific phosphatase activity against 5'-amp ... | 2000 | 11040429 |
| recurrent familial neonatal deaths: hereditary surfactant protein b deficiency. | hereditary surfactant protein b (sp-b) deficiency is an uncommon autosomal recessive lung disorder that causes hypoxemic respiratory failure in mature, morphologically normal infants. recognition and diagnosis of this condition is of paramount importance, as it has significant implications for future pregnancies with a recurrence risk of 25%. in a family with three neonatal deaths over 20 years, sp-b deficiency was diagnosed following the death of the fourth affected infant. previous deaths were ... | 2000 | 11041444 |
| induction of monocyte chemoattractant protein-1 by mycoplasma hominis in respiratory epithelial cells. | very small preterm infants who have genital mycoplasmas isolated from the trachea are at increased risk to develop bronchopulmonary dysplasia (bpd). the early stages of bpd are characterized by inflammation. recruitment and activation of mononuclear cells in response to mycoplasmas may be important in the early stage of the disease. lung epithelial cell production of monocyte chemoattractant protein-1 (mcp-1), a protein that attracts and activates mononuclear cells, could be critical in the regu ... | 2000 | 11094869 |
| mycoplasma hominis peritonitis after renal transplantation. | | 2000 | 11124622 |
| is mycoplasma hominis a vaginal pathogen? | to evaluate the role of mycoplasma hominis as a vaginal pathogen. | 2001 | 11158693 |
| hematoma infection with mycoplasma hominis following transplant nephrectomy. | | 2000 | 11168067 |
| mycoplasma hominis catheter-related infection in a patient with multiple trauma. | | 2000 | 11168084 |
| mycoplasma fermentans, m. hominis, and m. hyorhinis inhibit infectivity and growth of chlamydia trachomatis and c. pneumoniae in hep-2 cells. | | 2000 | 11184179 |
| [analysis of regions determining resistence to fluoroquinolones in genes gyra and parc in clinical isolates of mycoplasma hominis]. | fifteen strains of m. hominis isolated from patients with urogenital inflammations were analyzed. variations in the quinolone resistance-determining regions (qrdr) have been found in fluoroquinolone-resistant m. hominis clinical isolates in comparison with the reference pg21 strain. in one isolate, parc had asn substitute at position 91. | 2000 | 11186458 |
| immunoelectrophoretic analysis of medium-derived antigens bound to the surface of mycoplasma hominis, mycoplasma fermentans and ureaplasma urealyticum. | we analyzed mycoplasma hominis, mycoplasma fermentans and ureaplasma urealyticum antigens by immunochemical methods. the immunochemical reaction showed that all three species bound to the surface of one medium component, which origin stemmed from the horse serum. this substance functioned as one of the most potent components of mycoplasma immunogens for all three species. | 2000 | 11200673 |
| [comparison of various pcr methods for the detection of chlamydia trachomatis, ureaplasma urealyticum, and mycoplasma hominis in women with impaired reproductive function]. | the comparative evaluation of the pcr test "polimik" (research and production firm "litekh", moscow) and the pcr test of the novosibirsk institute of bioorganic chemistry (nibc) was carried out. the results obtained with the use of the pcr test "polimik" and the pcr test of the nibc of the detection of c. trachomatis and m. hominis coincided in 97.8% and 97.4% of cases. for u. urealyticum, the coincidence of the results of both pcr tests was 81.2%. among women who visited gynecologists for repro ... | 2000 | 11210648 |
| [cytotoxic activity of melittin expressed by recombinant vectors in acholeplasma laidlawii and mycoplasma hominis cells]. | recombinant plasmids containing the mellitin gene under the control of the lac and the tetm gene promoters were used for studying cytotoxic activity of mellitin in cells of mollicutes (mycoplasmas) and escherichia coli. after transformation of acholeplasma laidlawii and mycoplasma hominis cells with recombinant plasmid dnas by electroporation, cell growth was suppressed. the expression of the mellitin gene in a. laidlawii and m. hominis cells was demonstrated using the reverse transcription poly ... | 2001 | 11234424 |
| detection of cervical immunoglobulin a in normal pregnancy. | the aim of our study was to evaluate a feasible method to quantify the immunoglobulin a concentration in the cervical mucus of women with a normal singleton pregnancy. in 60 immunologic healthy pregnant women cervical mucus samples were taken at a random time in pregnancy using an absorbing cylindrical cotton-swab stick. in this cervical mucus immunoglobulin a concentration was measured by radial immunodiffusion. a vaginal bacterial swab was taken from each woman. concentration of immunoglobulin ... | 2001 | 11234615 |
| a cluster of mycoplasma hominis infection in heart-lung transplantation. | | 2001 | 11250419 |
| antimycoplasmal activities of (s)-(-)-9-fluoro-2,3-dihydro-3-methyl-10 -[4-(2-pyridyl)-1-piperazinyl]-7-oxo-7h-pyrido[1,2,3-de][1,4]benzoxazine -6-carboxylic acid (yh-6) in comparison with other antibiotics in vitro. | to determine the susceptibilities of mycoplasma and ureaplasma to (s)-(-)-9-fluoro-2,3-dihydro-3-methyl-10 -[4-(2-pyridyl)-1-piperazinyl]-7-oxo-7h-pyrido[1,2,3-de][1,4]benzoxazine -6-carboxylic acid (yh-6) and to compare it with those referential quinolones, macrolides, and tetracyclines. | 1999 | 11270970 |
| mycoplasma hominis and trichomonas vaginalis symbiosis: multiplicity of infection and transmissibility of m. hominis to human cells. | we recently reported that most trichomonas vaginalis isolates cultured in vitro are infected by mycoplasma hominis. in this work, we have characterized some aspects of the relationships between the two microorganisms. pcr, cultivation, and immunological methods revealed that the number of m. hominis organisms carried by t. vaginalis in culture varied from isolate to isolate, suggesting a specific multiplicity of infection. moreover, infected t. vaginalis isolates were able to pass bacteria not o ... | 2001 | 11271423 |
| [the influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation]. | nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. the aim of our study was to examine whether vaginal bacteria, especially those participating in bacterial vaginosis, are able to activate mast cells to mediators secretion. the study was done on rat peritoneal mast cells. the mast cells were incubated in vitro with suspensions of bacteroides capillo ... | 2000 | 11286181 |
| host and pathogen interaction during vaginal infection by trichomonas vaginalis and mycoplasma hominis or ureaplasma urealyticum. | vaginal infections by trichomonas vaginalis and mycoplasma hominis have been shown to be associated. since m. hominis and ureaplasma urealyticum are similar pathogens, both belonging to the class of the mycoplasmata, we describe here a molecular study into the interdependence of u. urealyticum and t. vaginalis during infection. susceptibility towards infection by u. urealyticum depends on genetic polymorphism in the interleukin-1 receptor antagonist (il-1ra) gene. now, we defined the relation be ... | 2001 | 11295198 |
| predictors of the vaginal microflora. | our purpose was to define influences on the patterns of the vaginal microflora. | 2001 | 11303191 |
| the diagnosis of bacterial vaginosis and vaginal flora changes. | in a population of 956 women, attending for contraceptive advice, 131 (13.7%) were found to have bv acording to amsel's criteria. clue cells were detected in 200 (20.9%) women, a positive amine ('sniff') test in 191 (20.0%), a vaginal ph > or = 4.7 in 243 (25.4%) and a "characteristic" vaginal discharge in 104 (10.9%) women. sensitivity, specificity, positive and negative predictive values in relation to bv were calculated for each of these four criteria. the detection of clue cells, an increase ... | 2001 | 11327086 |
| [microbiologic profile in symptomatic pregnant women's genital secretions in gran buenos aires, argentina]. | establish the prevalence of microorganisms associated with genital colonization in symptomatic pregnant women. in order to review the evolution of frequent pathogens ecology and adjust the laboratory design, in a population attended at the public health hospital, in the great buenos aires. | 2001 | 11333586 |
| vaginal microflora associated with bacterial vaginosis in nonpregnant women: reliability of sialidase detection. | to determine the prevalence of gardnerella vaginalis, anaerobic bacteria and mycoplasma hominis in vaginal specimens of women with and without bacterial vaginosis (bv) as well as to determine the sensitivity and specificity of the direct sialidase assay of vaginal fluid as a rapid test for diagnosing this syndrome. | 2001 | 11368254 |
| the detergent octylglucoside neutralizes lipopolysaccharide in a specific manner. | the stimulatory effect of lipopolysaccharide (lps) on human macrophages was found to be neutralized by the detergent octylglucoside (og). both macrophage stimulation and reactivity in a limulus amebocyte lysate test were suppressed by suspension of lps in og at concentrations between 0.25 and 2.5 mm, whereas other stimulatory lipopeptides and lipid containing stimulants were unaffected by og. lps at concentrations causing maximal stimulation of macrophages could be completely neutralized by non- ... | 2001 | 11388610 |
| [genital infection and infertility]. | genital infection is the most important cause of infertility worldwide, affecting not only fallopian tubes but all anatomic urogenital sites, both male and female. | 2001 | 11440663 |
| coupled phase-variable expression and epitope masking of selective surface lipoproteins increase surface phenotypic diversity in mycoplasma hominis. | a new mechanism expanding mycoplasmal surface diversity is described. exposure of surface epitopes on a constitutively expressed membrane protein (p56) of mycoplasma hominis was subject to high-frequency phase variation due to phase-variable expression of the p120 antigen and its selective masking of p56 epitopes. phase-variable masking may confer previously unrealized adaptive capabilities on mycoplasmas. | 2001 | 11447202 |
| the cytosolic hint protein of mycoplasma hominis interacts with two membrane proteins. | histidine triad nucleotide-binding (hint) proteins are dimeric proteins that bind to purines and are found in all three kingdoms: the eukarya, bacteria and archaea. in eukaryotes, hint proteins have been detected intracellularly, but their function is unknown. until now, knowledge about hint proteins in prokaryotes was restricted to sequence similarities and nucleotide-binding studies. in this study, we provide evidence that, in the cell wall-less prokaryote, mycoplasma hominis, the gene encodin ... | 2001 | 11454219 |
| frequency of mycoplasma hominis and ureaplasma urealyticum infections in women with systemic lupus erythematosus. | ureaplasma urealyticum (uu) and mycoplasma hominis (mh) have been detected in the urine of women with systemic lupus erythematosus (sle). we evaluated the presence of these mycoplasma in the endocervix of women presenting sle. a total of 40 sle patients (mean age 40.2 years), and 51 healthy women (mean age 30.9 years), were studied. endocervical swabs were cultured in specific liquid media for mh or uu, detected by a quantitative color assay, and considered positive at >10(3) dilutions. statisti ... | 2001 | 11460209 |
| is mycoplasma hominis a vaginal pathogen? | | 2001 | 11463941 |
| association of mycoplasma genitalium persistence in the urethra with recurrence of nongonococcal urethritis. | most patients with recurrent symptomatic nongonococcal urethritis receive negative test results for chlamydia trachomatis and ureaplasma urealyticum, and the cause of such recurrence usually is unknown. | 2001 | 11473221 |
| phylogeny of the seal mycoplasmas mycoplasma phocae corrig., mycoplasma phocicerebrale corrig. and mycoplasma phocirhinis corrig. based on sequence analysis of 16s rdna. | the nucleotide sequences of the 16s rrna genes from the type strains of three seal mycoplasmas, mycoplasma phocicerebrale, mycoplasma phocae and mycoplasma phocirhinis (formerly mycoplasma phocacerebrale, mycoplasma phocidae and mycoplasma phocarhinis, respectively), were determined by direct dna cycle sequencing. polymorphisms were found in all three 16s rrna gene sequences, showing the existence of two different rrna operons. in m. phocae, a length difference was found between the operons, cau ... | 2001 | 11491337 |
| susceptibilities of mycoplasma hominis, m. pneumoniae, and ureaplasma urealyticum to gar-936, dalfopristin, dirithromycin, evernimicin, gatifloxacin, linezolid, moxifloxacin, quinupristin-dalfopristin, and telithromycin compared to their susceptibilities to reference macrolides, tetracyclines, and quinolones. | the susceptibilities of mycoplasma hominis, mycoplasma pneumoniae, and ureaplasma urealyticum to eight new antimicrobial agents were determined by agar dilution. m. pneumoniae was susceptible to the new glycylcycline gar-936 at 0.12 microg/ml and evernimicin at 4 microg/ml, but it was resistant to linezolid. it was most susceptible to dirithromycin, quinupristin-dalfopristin, telithromycin, reference macrolides, and josamycin. m. hominis was susceptible to linezolid, evernimicin, and gar-936. it ... | 2001 | 11502536 |
| serological investigation of mycoplasma genitalium in infertile women. | the role of mycoplasma genitalium in the pathogenesis of pelvic inflammatory disease has not been characterized. | 2001 | 11527890 |
| two new point mutations at a2062 associated with resistance to 16-membered macrolide antibiotics in mutant strains of mycoplasma hominis. | we describe two mutants of mycoplasma hominis pg-21 which show resistance to 16-membered macrolides but susceptibility to lincosamides, obtained by in vitro exposure to increasing doses of josamycin. the 23s rrna gene showed that each had a mutation (a2062g and a2062t) corresponding to nucleotide 2062 in escherichia coli, which was associated with the acquired phenotype. | 2001 | 11557502 |
| systemic mycoplasma hominis infection in a patient immunocompromised due to combined transplantation of kidney and pancreas. | | 2001 | 11561812 |
| relationship of abnormal vaginal flora, proinflammatory cytokines and idiopathic infertility in women undergoing ivf. | to investigate the prevalence of bacterial vaginosis (bv) and abnormal bacterial vaginal flora in an infertile population and correlate with cervical cytokine production and in vitro fertilization (ivf) outcome. | 2001 | 11584481 |
| genetic heterogeneity of mycoplasma hominis clinical isolates detected during observation of patients with recurrent urogenital inflammation. | a rapid reproducible effective method for molecular typing of mycoplasma hominis strains based on random amplified polymorphic dna (rapd) technique was developed. rapd detected genetic heterogeneity of genomes of mycoplasma hominis clinical isolates and showed changes in the genomes of mycoplasma hominis clinical isolates from patients with chronic infection. | 2001 | 11687848 |
| the p100 coactivator is present in the nuclei of mammary epithelial cells and its abundance is increased in response to prolactin in culture and in mammary tissue during lactation. | the p100 coactivator, first identified as a coactivator of the epstein-barr virus-encoded transcription factor, ebna-2, in cultured cells, interacts with a number of transcription factors. however, the role of p100 in animals is unclear. we found that the abundance of p100 is closely associated with the lactating state in mammary tissue of mice and cows. using two antibodies against independent parts of the protein, p100 immunoreactivity was localised to mammary epithelial cells, and was enriche ... | 2001 | 11691653 |
| effect of controlled expression of the melittin gene on infection caused by mycoplasma hominis in cell culture. | | 2001 | 11712175 |
| molecular design of mycoplasma hominis vaa adhesin. | the variable adherence-associated (vaa) adhesin of the opportunistic human pathogen mycoplasma hominis is a surface-exposed, membrane-associated protein involved in the attachment of the bacterium to host cells. the molecular masses of recombinant 1 and 2 cassette forms of the protein determined by a light-scattering (ls) method were 23.9 kd and 36.5 kd, respectively, and corresponded to their monomeric forms. circular dichroism (cd) spectroscopy of the full-length forms indicated that the vaa p ... | 2001 | 11714926 |
| evaluation of bact/alert system for detection of mycoplasma hominis in simulated blood cultures. | we used simulated blood cultures inoculated with clinical isolates of mycoplasma hominis to determine whether liquid media of the bact/alert (organon teknika, durham, n.c.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. viability of clinical isolates of m. hominis was maintained for 7 days in bact/alert media, and organisms were able to multiply when 1% gelatin was added to neutralize the mycoplasmastatic effects of th ... | 2001 | 11724840 |
| identification and quantification of ureaplasmas colonizing the respiratory tract and assessment of their role in the development of chronic lung disease in preterm infants. | the role of ureaplasma urealyticum in the development of chronic lung disease (cld) in preterm infants continues to be disputed. recently u. urealyticum has been found to consist of two species, u. urealyticum and ureaplasma parvum, a finding that has not been considered in previous studies of cld. this study examined the possible relationships between development of cld and respiratory colonization by these newly redefined species, their concentrations in lower respiratory secretions and the ef ... | 2001 | 11734763 |
| a clinical study on the association of trichomonas vaginalis and mycoplasma hominis infections in women attending a sexually transmitted disease (std) outpatient clinic. | swabs from the posterior vaginal fornix were obtained from 804 consecutive female patients visiting a large dutch sexually transmitted diseases (std) outpatient clinic. a detailed clinical history was obtained and complaints concerning the lower genital tract, such as vaginal discharge or vulval and vaginal irritation, were recorded. patients were examined and the presence of non-physiological vaginal secretions was established by speculum examination. the swabs were monitored for bacterial vagi ... | 2001 | 11750218 |
| production of pro- and anti-inflammatory cytokines of human placental trophoblasts in response to pathogenic bacteria. | we studied the production of cytokines in purified cultures of human term trophoblasts in the presence of pathogenic strains of escherichia coli, bacteroides fragilis, mycoplasma hominis, staphylococcus aureus, and streptococcus agalactiae, which have been identified in intrauterine infections. | 2001 | 11750868 |
| phylogeny-based rapid identification of mycoplasmas and ureaplasmas from urethritis patients. | some strains of mycoplasmas and ureaplasmas (family mycoplasmataceae) are associated with nongonococcal urethritis (ngu) or other genitourinary infections. we have developed a rapid and reliable method of identifying the presence and prevalence of mycoplasmas and ureaplasmas in men with ngu. this method is based on the amplification of a part of the 16s rrna gene by pcr and phylogenetic analysis. a portion of the 16s rrna gene from 15 prototype strains was amplified with a set of common primers, ... | 2002 | 11773101 |
| genital mycoplasmas, including mycoplasma genitalium, as sexually transmitted agents. | mycoplasmas are the smallest free-living organisms, widespread in nature. several mycoplasma species have been isolated from humans. for 6 of them: mycoplasma hominis, ureaplasma urealyticum, m. primatum, m. genitalium, m. spermatophilum and m. penetrans, the genital tract is the main site of colonization. this review is concentrated on the role of mycoplasmas as sexually transmitted agents, with the emphasis to m. genitalium infections. m. hominis and u. urealyticum are isolated from the genita ... | 2002 | 11839161 |
| induced expression of melittin, an antimicrobial peptide, inhibits infection by chlamydia trachomatis and mycoplasma hominis in a hela cell line. | as the number of pathogenic microbial strains resistant to different antibiotics increases, amphipathic peptides with antimicrobial activity are promising agents for the therapy of infectious diseases. this work deals with the effect of an amphipathic antimicrobial peptide, melittin, expressed within recombinant plasmid vectors, on infection with urogenital pathogens chlamydia trachomatis and mycoplasma hominis in hela cell culture. recombinant plasmid constructs with the melittin gene under the ... | 2002 | 11850166 |
| evidence of active efflux in resistance to ciprofloxacin and to ethidium bromide by mycoplasma hominis. | the uptake of fluoroquinolones was characterized for the fluoroquinolone-susceptible strain pg21 of mycoplasma hominis. accumulation of fluoroquinolones appeared to occur by passive diffusion. addition of arginine as the energizer significantly reduced the uptake of fluoroquinolones, suggesting the presence of an energy-dependent efflux process. reserpine and orthovanadate, two multidrug pump inhibitors, increased significantly the ciprofloxacin (cip) uptake. in contrast, such a strong effect wa ... | 2002 | 11850247 |
| meningo-encephalitis in a neonate caused by maternal mycoplasma hominis treated successfully with chloramphenicol. | | 2002 | 11863270 |
| mycoplasma hominis infection in renal transplantation. | | 2002 | 11865099 |
| intrauterine infection and prematurity. | intrauterine infection is a major cause of premature labor with and without intact membranes. intrauterine infection is present in approximately 25% of all preterm births and the earlier the gestational age at delivery, the higher the frequency of intra-amniotic infection. microorganisms may also gain access to the fetus before delivery. a fetal inflammatory response syndrome elicited in response to microbial products is associated with the impending onset of preterm labor and also with multi-sy ... | 2002 | 11921380 |
| ureaplasma urealyticum biovar determination in women attending a family planning clinic in guiné-bissau, using polymerase chain reaction of the multiple-banded antigen gene. | although ureaplasma urealyticum is commonly found in the genital tract of asymptomatic women, it has been suggested that only certain subgroups of this microorganism are disease associated. vaginal specimens were collected to determine the distribution of u. urealyticum biovars and to estimate their possible association with age, absence of lactobacilli, and tetracycline resistance. of the 94 women studied, 40 (43%) carried u. urealyticum and were biotyped by polymerase chain reaction (pcr). twe ... | 2002 | 11948794 |
| [study on etiology of nongonococcal urethritis]. | one thousand one hundred and fifty-seven samples from urogenital tract were detected by isolating culture and immunofluorescence. the positive rates of chlamydia trachomatis (ct), ureaplasma urealyticum (uu), mycoplasma hominis (mh), candida (cd), staphylococcus aureus were 27.8%, 33.5%, 25.6%, 11.5%, 23% respectively. the rate of mixed infection caused by at least two pathogens was 19.8%. about 90% of the infected patients were from 20 to 40 years old. the related factors of pathogen detection ... | 1999 | 12016794 |
| preterm labour--is mycoplasma hominis involved? | to assess whether mycoplasma hominis is associated with preterm labour in primigravidae and multigravidae with previous midtrimester abortion or preterm labour. | 2002 | 12040954 |
| [cervicovaginal infection as a risk factor for premature labor]. | to identify the possible association between cervicovaginal infections (cvi) and preterm delivery. | 2002 | 12050955 |
| characterization of the mycoplasma hominis ftsz gene and its sequence variability in mycoplasma clinical isolates. | we cloned and sequenced mycoplasma hominis chromosomal fragment containing ftsz gene. the wild-type expression of the gene was shown at rna level by reverse transcription followed by pcr amplification. we revealed that m. hominis ftsz had a comparatively low similarity to proteins of mycoplasma genitalium and mycoplasma pneumoniae. after full ftsz gene sequencing for 14 clinical isolates of m. hominis, single-nucleotide substitutions were found in 21 positions, 6 of them being common for almost ... | 2002 | 12054578 |
| [antigenic variation in mycoplasmas]. | | 2002 | 12102047 |
| [cervico-vaginal infections. study of a population in the turin area]. | aim of this study is to determine the prevalence, in the turin area, of the pathogens chiefly involved in the genesis of the most common infections of the female genitalia. | 2002 | 12114863 |
| [development of fluoroquinolone (ciprofloxacin) resistance in mycoplasma hominis in the presence of hela cells]. | the effect of cocultivation of eukaryotic hela cells and mycoplasma hominis mycoplasma on the resistance of the latter to fluoroquinolones (ciprofloxacin) was examined. it was shown that cocultivation of the m. homonis and hela cells during 24 h with subsequent addition of ciprofloxacin resulted in an increase of the mircoplasma resistance to this antimicrobial agent. in the m. hominis cells cultivated in the presence of hela cells and the increasing concentration of ciprofloxacin mutations in t ... | 2002 | 12174584 |
| the etiology of pelvic inflammatory disease. | the etiology of pelvic inflammatory disease (pid) is speculated upon based on reported incidence and epidemiological studies. in western society, the incidence of pid (annual) is 1% among women aged 15-34 years and 2% in the high risk group of women aged 15-24 years. the annual incidence in the us is higher, at least 2% among fecund sexually active women aged 13-44 years. the medical consequences of pid are infertility, ectopic pregnancy, and chronic pelvic pain. causative agents include nei ... | 1984 | 12179634 |
| mycoplasma genitalium is not associated with adverse outcomes of pregnancy in guinea-bissau. | to evaluate the impact of mycoplasma genitalium on the outcome of pregnancy. | 2002 | 12181470 |
| identification and functional mapping of the mycoplasma fermentans p29 adhesin. | initial adherence interactions between mycoplasmas and mammalian cells are important for host colonization and may contribute to subsequent pathogenic processes. despite significant progress toward understanding the role of specialized, complex tip structures in the adherence of some mycoplasmas, particularly those that infect humans, less is known about adhesins through which other mycoplasmas of this host bind to diverse cell types, even though simpler surface components are likely to be invol ... | 2002 | 12183538 |
| pelvic inflammatory disease in the people's republic of china: aetiology and management. | to study the causes of pelvic inflammatory disease (pid) in shenyang, northeastern china, and to assess the efficacy of the syndromic management flowchart for pid recommended by world health organization (who). | 2002 | 12194742 |
| safety and tolerability of the new contraceptive sponge protectaid. | this study was undertaken to assess the efficacy, safety and acceptability of two different-dose regimens of the protectaid contraceptive sponge. | 2002 | 12201327 |
| characterization of p40, a cytadhesin of mycoplasma agalactiae. | an immunodominant protein, p40, of mycoplasma agalactiae was analyzed genetically and functionally. the gene encoding p40 was cloned from type strain pg2, sequenced, submitted to point mutagenesis in order to convert mycoplasma-specific tga(trp) codon to the universal tgg(trp) codon, and subsequently expressed in escherichia coli. nucleotide sequence-derived amino acid sequence comparisons revealed a similarity of p40 to the adhesin p50 of mycoplasma hominis and to protein p89 of spiroplasma cit ... | 2002 | 12228289 |
| mutations in 23s rrna account for intrinsic resistance to macrolides in mycoplasma hominis and mycoplasma fermentans and for acquired resistance to macrolides in m. hominis. | the mechanisms of intrinsic resistance of mycoplasma hominis to 14- and 15-membered macrolides were investigated in comparison with those of m. pneumoniae, which is naturally susceptible to macrolides. radiolabeled erythromycin was not accumulated by m. hominis pg21, but addition of an abc transporter inhibitor increased the level of erythromycin uptake more than two times, suggesting the existence of an active efflux process. the affinity of [(14)c]erythromycin to ribosomes isolated from m. hom ... | 2002 | 12234836 |