| 2016 european guideline on the management of non-gonococcal urethritis. | we present the updated international union against sexually transmitted infections (iusti) guideline for the management of non-gonococcal urethritis in men. this guideline recommends confirmation of urethritis in symptomatic men before starting treatment. it does not recommend testing asymptomatic men for the presence of urethritis. all men with urethritis should be tested for chlamydia trachomatis and neisseria gonorrhoeae and ideally mycoplasma genitalium using a nucleic acid amplification tes ... | 2016 | 27147267 |
| evaluation of the bio-rad dx ct/ng/mg® assay for simultaneous detection of chlamydia trachomatis, neisseria gonorrhoeae and mycoplasma genitalium in urine. | we evaluated the performance of the bio-rad real-time dx ct/ng/mg® assay for detection of c. trachomatis, n. gonorrhoeae and m. genitalium on a collection of 441 urine samples from sexually transmitted infections, or travellers consultations and from anonymous sperm donors that were previously analysed with the abbott realtime ct/ng assay. samples positive for c. trachomatis or n. gonorrhoeae with the abbott assay had all previously been confirmed with an in-house real-time pcr assay. samples po ... | 2016 | 27116008 |
| is the risk for sexually transmissible infections (sti) lower among women with exclusively female sexual partners compared with women with male partners? a retrospective study based on attendees at a norwegian sti clinic from 2004 to 2014. | the prevalence of and the risk for sexually transmissible infections (stis) for women engaging in same-sex sexual behaviour was investigated among women attending an sti clinic. | 2016 | 27050292 |
| detection of hpv and co-infecting pathogens in healthy italian women by multiplex real-time pcr. | several pathogens can be transmitted sexually and are an important cause of morbidity among sexually active women. the aim of the study was to detect the presence of human papillomavirus (hpv), chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), trichomonas vaginalis (tv), mycoplasma hominis (mh), mycoplasma genitalium (mg), ureaplasma urealyticum (uu), and ureaplasma parvum (up) in a group of 309 healthy women enrolled at the san camillo - forlanini hospital of rome by using two multiplex r ... | 2016 | 27031891 |
| the first report: an analysis of bacterial flora of the first voided urine specimens of patients with male urethritis using the 16s ribosomal rna gene-based clone library method. | to analyse the bacterial flora of urine from patients with male urethritis using the clone library method. | 2016 | 27013259 |
| ureaplasma urealyticum: presence among sexually transmitted diseases. | the aim of this study was to detect the presence of chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, trichomonas vaginalis, and ureaplasma urealyticum in genital specimens of symptomatic patients. this study also examined the role of u. urealyticum in infections of the lower genital tract. cervical and urethral samples from 96 patients (46 males, 50 females) were tested using the seeplex((®)) std6 ace kit. consent forms were received and a questionnaire wa ... | 2017 | 27000449 |
| periodic presumptive treatment for vaginal infections may reduce the incidence of sexually transmitted bacterial infections. | bacterial vaginosis (bv) may increase women's susceptibility to sexually transmitted infections (stis). in a randomized trial of periodic presumptive treatment (ppt) to reduce vaginal infections, we observed a significant reduction in bv. we further assessed the intervention effect on incident chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma genitalium infection. | 2016 | 26908758 |
| prevalence of chlamydia trachomatis, ureaplasma spp., mycoplasma genitalium and mycoplasma hominis among outpatients in central greece: absence of tetracycline resistance gene tet(m) over a 4-year period study. | a total of 301 men and women attending local urologists and gynaecologists in the state of thessaly, central greece, were tested for chlamydia trachomatis, ureaplasma spp., mycoplasma genitalium and mycoplasma hominis dna. investigation of the tet(m) gene, which confers tetracycline resistance in these genera, was also performed. low incidence of c. trachomatis and mycoplasma spp. as well as high prevalence of ureaplasma spp., especially among women, were found. the tet(m) gene was absent in all ... | 2016 | 26862428 |
| male non-gonococcal urethritis: from microbiological etiologies to demographic and clinical features. | to detect microorganisms responsible for male acute urethritis and to define the microbiology of non-gonococcal urethritis. | 2016 | 26845624 |
| prevalence of mycoplasma genitalium in men with urethritis and in high risk asymptomatic males in tel aviv: a prospective study. | we sought to investigate the prevalence of mycoplasma genitalium, chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis in men presenting to a sexually transmitted infection (sti) clinic, with special attention to m. genitalium, its occurrence in israeli patients, coinfections, and risk factors. in a cross-sectional study, 259 men were successively enrolled in the tel aviv levinsky clinic for stis between november 2008 and november 2010. there were 118 men with urethritis and 1 ... | 2017 | 26826161 |
| prevalence of m. genitalium and u. urealyticum in urine tested for c. trachomatis. | mycoplasma genitalium and ureaplasma urealyticum cause sexually transmitted infections. while m. genitalium is an established aetiological agent, u. urealyticum is still controversial as a pathogen. testing for these microbes is not yet widely available in norway, and knowledge of their prevalence is limited. in this study we have investigated the prevalence of m. genitalium and u. urealyticum in a heterogeneous population from vestfold and telemark. | 2016 | 26813816 |
| cervicitis: a prospective observational study of empiric azithromycin treatment in women with cervicitis and non-specific cervicitis. | treatment with 1 g azithromycin was observed prospectively in 130 women with cervicitis (>30 polymorphonuclear leucocytes/high-powered field) enrolled in a cervicitis aetiology study of 558 women at three sexually transmitted infection clinics in sydney, australia. two overlapping groups of women with cervicitis were considered: 'cervicitis group 1' (n = 116) excluded women with trichomonas vaginalis and a subgroup of this, 'cervicitis group 2' (non-specific cervicitis) (n = 96) further excluded ... | 2017 | 26792283 |
| analytical performance of multiplex real-time pcr for six sexually transmitted pathogens. | most organisms that cause sexual transmitted diseases (stds) are fastidious pathogens that are difficult to detect with conventional microbiological methods and the proportions of multiple infections were noted up to 39.3% among the sti-positive subjects. however, only a few multiplex pcr and multiplex real-time pcr tests that can screen more than six microorganisms that cause stds have been assessed. | 2015 | 26732001 |
| usefulness of a novel multiplex real-time pcr assay for the diagnosis of sexually-transmitted infections. | sexually transmitted infections (sti) are currently on the increase worldwide. new molecular tools have been developed in the past few years in order to improve their diagnosis. an evaluation was carried out using a new commercially available real-time pcr assay, anyplex™ ii sti-7 (seegene, seoul, korea), which detects seven major pathogens in a single reaction - chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, mycoplasma hominis, mycoplasma genitalium, ureaplasma urealyticum ... | 2016 | 26706392 |
| urethritis-associated pathogens in urine from men with non-gonococcal urethritis: a case-control study. | the aetiology of non-gonococcal urethritis (ngu) remains unexplained in 30-40% of patients. urine samples from men attending swedish sexually transmitted disease clinics were examined by species-specific quantitative pcrs for chlamydia trachomatis, mycoplasma genitalium, trichomonas vaginalis, ureaplasma urealyticum, u. parvum, adenovirus, herpes simplex virus, neisseria meningitidis, haemophilus influenzae, moraxella catarrhalis and streptococcus pneumoniae. a total of 187 men with acute ngu (s ... | 2016 | 26658669 |
| sexually transmitted infections in children as a marker of child sexual abuse and direction of future research. | this review considers recent evidence on sexually transmitted infections (stis) as a marker of child sexual abuse (csa), when diagnosed after the neonatal period. it also aims to identify if there are specific areas where additional research is required. | 2016 | 26658657 |
| the prevalence of causative organisms of community-acquired urethritis in an age group at high risk for sexually transmitted infections in korean soldiers. | this study was designed to evaluate the causative organisms in young male soldiers with clinical signs and symptoms after sexual contact that suggests a diagnosis of urethritis. | 2017 | 26607860 |
| advances in the understanding and treatment of male urethritis. | neisseria gonorrhoeae and chlamydia trachomatis are well-documented urethral pathogens, and the literature supporting mycoplasma genitalium as an etiology of urethritis is growing. trichomonas vaginalis and viral pathogens (herpes simplex virus types 1 and 2 and adenovirus) can cause urethritis, particularly in specific subpopulations. new data are emerging regarding the potential role of bacterial vaginosis-associated bacteria in urethritis, although results are inconsistent regarding the patho ... | 2015 | 26602615 |
| cervicitis aetiology and case definition: a study in australian women attending sexually transmitted infection clinics. | studies examining cervicitis aetiology and prevalence lack comparability due to varying criteria for cervicitis. we aimed to outline cervicitis associations and suggest a best case definition. | 2016 | 26586777 |
| asymptomatic anal sexually transmitted infections in hiv-positive men attending anal cancer screening. | hiv-positive men who have sex with men (hiv+msm) have an increased risk for anal dysplasia and for sexually transmitted infections (stis). | 2016 | 26577338 |
| pelvic inflammatory disease associated with chlamydia trachomatis but not mycoplasma genitalium in new zealand. | background there is a paucity of studies looking at associations between mycoplasma genitalium and pelvic inflammatory disease (pid). the objectives of this study were to estimate the prevalence of m. genitalium in women attending a sexual health service in new zealand and secondly to examine for an association of m. genitalium with pid. | 2016 | 26547264 |
| multiplex pcr testing for nine different sexually transmitted infections. | current sexually transmitted infection (sti) testing is not optimal due to delays in reporting or missed diagnoses due to a lack of comprehensive testing. the filmarray® (biofire diagnostics, llc, salt lake city, utah) is a user-friendly, fully automated, multiplex pcr system that is being developed for rapid point-of-care use. a research-use-only sti panel including multiple pcr primer sets for each organism was designed to detect chlamydia trachomatis, neisseria gonorrhoeae, treponema pallidum ... | 2016 | 26538551 |
| ureaplasma parvum and mycoplasma genitalium are found to be significantly associated with microscopy-confirmed urethritis in a routine genitourinary medicine setting. | summaryinflammation of the urethra defined by an excess of polymorphonuclear leukocytes in the absence of sexually transmitted chlamydia trachomatis and neisseria gonorrhoeae is called non-chlamydial non-gonococcal urethritis (ncngu). although mycoplasma genitalium is now recognised as causing a sexually transmitted infection, the clinical significance of the other mollicute species is less clear. this study used specific real-time quantitative polymerase chain reaction assays to detect and quan ... | 2016 | 26378187 |
| evaluation of the new amplisens multiplex real-time pcr assay for simultaneous detection of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, and trichomonas vaginalis. | in this study, we performed an evaluation of the new ce-marked multiplex real-time amplisens n.gonorrhoeae/c.trachomatis/m.genitalium/t.vaginalis-multiprime-frt pcr assay compared to aptima tests, i.e., aptima combo 2 assay, aptima trichomonas vaginalis assay (fda-approved), and two different aptima mycoplasma genitalium assays (research use only; one of them only used for discrepancy analysis). vaginal swabs (n = 209) and first-void urine (fvu) specimens from females (n = 498) and males (n = 55 ... | 2015 | 26299582 |
| trichomonas vaginalis and mycoplasma genitalium: age-specific prevalence and disease burden in men attending a sexually transmitted infections clinic in amsterdam, the netherlands. | men are not routinely tested for trichomonas vaginalis (tv) and mycoplasma genitalium (mg) in the netherlands and, therefore, very few studies have looked into their prevalence and/or role in urogenital complaints in the dutch male population. | 2016 | 26283740 |
| large two-centre study into the prevalence of mycoplasma genitalium and trichomonas vaginalis in the netherlands. | mycoplasma genitalium and trichomonas vaginalis are common sexually transmitted infections (stis). in the netherlands, testing for m. genitalium and t. vaginalis is not recommended for first-line sti screening. recent reports about the increasing antimicrobial resistance in m. genitalium raise concern about the adequacy of current empirical treatment regimens. it is necessary to have insight in the prevalence of m. genitalium and t. vaginalis in order to evaluate current first-line sti screening ... | 2016 | 26199286 |
| a phase 2 trial of oral solithromycin 1200 mg or 1000 mg as single-dose oral therapy for uncomplicated gonorrhea. | progressive resistance to antimicrobial agents has reduced options for gonorrhea therapy worldwide. solithromycin (cem-101) is a novel oral fluoroketolide antimicrobial with substantial in vitro activity against neisseria gonorrhoeae. | 2015 | 26089222 |
| male circumcision and sti acquisition in britain: evidence from a national probability sample survey. | it is well-established that male circumcision reduces acquisition of hiv, herpes simplex virus 2, chancroid, and syphilis. however, the effect on the acquisition of non-ulcerative sexually transmitted infections (stis) remains unclear. we examined the relationship between circumcision and biological measures of three stis: human papillomavirus (hpv), chlamydia trachomatis and mycoplasma genitalium. | 2015 | 26083250 |
| prevalence of sexually transmitted pathogens associated with hpv infection in cervical samples in a mexican population. | cervical cancer development has been mainly associated with persistent human papillomavirus (hpv) infections. however, hpv infection is unlikely to be sufficient to cause cervical cancer, and the contribution of other sexually transmitted infections (stis) could be the determining factor for cervical lesion-progression. the aim of this study was to estimate the prevalence of stis associated with hpv-positivity in 201 cervical samples from patients who underwent annual routine gynecological exams ... | 2015 | 26010580 |
| 2015 uk national guideline on the management of non-gonococcal urethritis. | we present the updated british association for sexual health and hiv guideline for the management of non-gonococcal urethritis in men. this document includes a review of the current literature on its aetiology, diagnosis and management. in particular it highlights the emerging evidence that azithromycin 1 g may result in the development of antimicrobial resistance in mycoplasma genitalium and that neither azithromycin 1 g nor doxycycline 100 mg twice daily for seven days achieves a cure rate of ... | 2016 | 26002319 |
| the role of chlamydia trachomatis in high-risk human papillomavirus persistence among female sex workers in nairobi, kenya. | little is known about risk factors for persistent high-risk human papillomavirus (hrhpv) infection in low-income settings, and prior research has not quantified the relative duration of hrhpv infections stratified by risk factors. we compared the duration of hrhpv infection among female sex workers (fsws) by exposure to sexually transmitted infections (stis), using a highly sensitive biomarker assay. | 2015 | 25970306 |
| detection of mycoplasma genitalium from male primary urine specimens: an epidemiologic dichotomy with trichomonas vaginalis. | a total of 2750 male urines subjected to a transcription-mediated amplification (tma)-based mycoplasma genitalium assay yielded 188 positive results (6.84%). this rate was similar to chlamydia trachomatis (6.87%; p = 0.96) and greater than neisseria gonorrhoeae (4.0%) and trichomonas vaginalis (2.3%; p < 0.0002). mean age of m. genitalium-infected males (30.8) was similar to n. gonorrhoeae (p = 0.78) but less than t. vaginalis (mean, 41.6; p < 0.0001). a total of 266 sti clinic encounters had at ... | 2015 | 25934156 |
| mycoplasma genitalium serum antibodies in infertile couples and fertile women. | the association between mycoplasma genitalium (m. genitalium) serum antibodies and infertility in women and men, as well as infertility subtypes, was investigated. | 2015 | 25921018 |
| five-day azithromycin treatment regimen for mycoplasma genitalium infection also effectively eradicates chlamydia trachomatis. | | 2015 | 25823977 |
| screening for chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma genitalium should it be integrated into routine pregnancy care in french young pregnant women? | chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma genitalium screening during pregnancy is not performed routinely in france. we conducted the first prospective study in 1004 women attending for routine antenatal care to determine the prevalence and risk factors for these bacterial infections. the overall prevalence of c. trachomatis, n. gonorrhoeae, and m. genitalium infections was 2.5%, 0%, and 0.8%, respectively. in patients aged 18-24 years, the prevalence increased to 7.9% for c. ... | 2015 | 25753079 |
| opportunities and pitfalls of molecular testing for detecting sexually transmitted pathogens. | in the last 20 years, nucleic acid amplification tests (naats) have gradually replaced traditional methods for the detection of sexually transmitted infections. naat technology comes with some considerable benefits for diagnosis, including increased sensitivity, rapid result turnaround and suitability for high throughput screening of asymptomatic individuals using more-readily available specimens. however, the transition to naat has not come without its problems. false-negative and false-positiv ... | 2015 | 25714587 |
| trichomonas vaginalis infection: how significant is it in men presenting with recurrent or persistent symptoms of urethritis? | persistent or recurrent non-gonococcal urethritis has been reported to affect up to 10-20% of men attending sexual health clinics. an audit was undertaken to review the management of persistent or recurrent non-gonococcal urethritis in men presenting at whittall street clinic, birmingham, uk. detection of trichomonas vaginalis infection was with the newly-introduced nucleic acid amplification test. a total of 43 (8%) of 533 men treated for urethritis re-attended within three months with persiste ... | 2016 | 25663246 |
| etiology and antimicrobial susceptibility of pathogens responsible for urethral discharge among men in harare, zimbabwe. | periodic etiological surveillance of sexually transmitted infection (sti) syndromes is required to validate treatment algorithms used to control stis. however, such surveys have not been performed in zimbabwe over the past decade. | 2014 | 25581806 |
| [some diagnostic aspects of chlamydia trachomatis urogenital infections]. | the following tests were used to make the diagnosis of chlamydia trachomatis: ensime immunoassay (eia), enzyme-linked fluorescentassay (elfa), polymerase chain reaction dna amplification for chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium and ureaplasma urealyticum. the subject of this study were 486 outpatients between the ages of 17 and 62, diagnosed with urethritis, epidydimitis, prostatitis, cervicitis, endocervicitis, pelvic inflammatory disease and sterility. comparison ... | 2015 | 27032228 |
| diagnosis of common bacterial causes of urethritis in men by gram stain, culture and multiplex pcr. | urethritis is one of the most important causes of morbidity and mortality in developing countries. the aim of this study was to detect common bacterial causes of urethritis in men by gram stain, culture and multiplex pcr.185 male patients who presented at the skin and venereal clinic of the dhaka medical college, bangladesh with clinical symptoms suggestive of urethritis were enrolled in this study. urethral discharges were tested for detection of neisseria gonorrhoeae by gram stain, culture and ... | 2014 | 25500516 |
| prevalence of chlamydia trachomatis, neisseria gonorrhoeae and mycoplasma genitalium in asymptomatic patients under 30 years of age screened in a french sexually transmitted infections clinic. | an increasing prevalence of sexually transmitted infections (sti) has been noted in france over the past decade. asymptomatic carriage may be high in patients infected with chlamydia trachomatis attending free and anonymous screening centres (cdag) and information, diagnosis and screening centres for sti (ciddist). in these centres, systematic c. trachomatis detection is recommended in women ≤25 years and in men ≤30 years. | 2014 | 25322708 |
| screening for chlamydia trachomatis and mycoplasma genitalium; is first void urine or genital swab best? | | 2015 | 25253762 |
| epidemiology of sexually transmitted infections in rural haitian men. | the study attempts to determine the prevalence of organisms associated with urethritis in men in rural southwestern haiti and to determine the association with demographic, clinical and laboratory variables. a standardised verbal interview was conducted; genital examinations were done; urethral swabs were collected for nucleic acid amplification testing, and first void urine was obtained for urinalysis. the mean participant age was 54; 88.8% lived in a rural area. swabs were positive for trichom ... | 2015 | 25228665 |
| epidemiology of sexually transmitted infections in rural southwestern haiti: the grand'anse women's health study. | the study attempts to define socioeconomic, clinical, and laboratory correlates in vaginitis and other sexually transmitted infections in rural southwestern haiti. a convenience sample of subjects recruited from a rural women's health clinic and attending an established clinic at the haitian health foundation (hhf) clinic was studied. a standardized history and physical examination, including speculum examination, and collection of blood, urine, and vaginal swabs were obtained from the women at ... | 2014 | 25200263 |
| usefulness of quantifying leukocytes in first-voided urine to predict positivity for chlamydia trachomatis in asymptomatic men at high risk for chlamydial infection. | chlamydia trachomatis causes acute non-gonococcal urethritis, but some infected men are asymptomatic. we examined leukocytes in uncentrifuged first-voided urine (fvu) from asymptomatic men at high risk for chlamydial infection by automated urine particle analyzers to assess whether the quantification of urinary leukocytes could predict chlamydial infection in these men. we enrolled 209 asymptomatic men, whose female sexual partners had been diagnosed as having a genital chlamydial infection. the ... | 2014 | 25156010 |
| detection of infectious organisms in archival prostate cancer tissues. | seroepidemiological studies have reported associations between exposure to sexually transmitted organisms and prostate cancer risk. this study sought dna evidence of candidate organisms in archival prostate cancer tissues with the aim of assessing if a subset of these cancers show any association with common genital infections. | 2014 | 25106851 |
| sexually transmitted infections in women. | sexually transmitted infections (stis) are highly prevalent and cause a wide spectrum of disease. however, the majority of these infections may be unrecognized due to lack of overt signs or symptoms of infection. asymptomatic infections remain significant as a result of the potential for long-term sequelae, predominately in women, and the risks of complications during pregnancy as well as mother-to-child transmission. laboratory diagnostics play an important role in identifying infection and in ... | 2014 | 25083897 |
| prevalence and determinants of sexually transmitted infections in women at risk undergoing abortion in a swiss primary care setting. | specific prevalence data are needed to assess the burden of sexually transmitted infections (stis) in specific settings where data are scant, such as women seeking early termination of pregnancy (top). we aimed to investigate the prevalence of stis and its determinants in women at risk (e. g. pathological cervical swab) undergoing top in a primary care setting in switzerland. | 2014 | 25051930 |
| prevalence of trichomonas vaginalis, mycoplasma genitalium and ureaplasma urealyticum in men with urethritis attending an urban sexual health clinic. | we conducted a study to determine the prevalence of trichomonas vaginalis (tv), mycoplasma genitalium (mg) and ureaplasma urealyticum (uu) in men with urethritis, attending an urban sexual health clinic, in order to inform screening and treatment policies. men attending an urban sexual health clinic between june 2011 and january 2012 were evaluated. urine samples were collected from men with urethritis and tested for chlamydia trachomatis (ct), neisseria gonorrhoeae (gc) and tv using transcripti ... | 2015 | 24925897 |
| diagnosis and antimicrobial treatment of mycoplasma genitalium infection: sobering thoughts. | the discovery of mycoplasma genitalium in 1980-1981 eventually led to it becoming recognized as an important cause of non-gonococcal urethritis in men and also some genital tract diseases in women. subsequent to the original isolation, further attempts failed over the next decade and reliable detection only became possible with the use of nucleic acid amplification techniques. although tetracyclines, particularly doxycycline, were the first choice for treatment of non-gonococcal urethritis prior ... | 2014 | 24834454 |
| distribution and perinatal transmission of bacterial vaginal infections in pregnant women without vaginal symptoms. | we evaluated the distribution and vertical transmission of bacterial vaginal infections in asymptomatic pregnant women. | 2014 | 24552584 |
| cervical cytopathological findings in korean women with chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum infections. | this is to investigate the cervical cytological abnormalities associated with chlamydia trachomatis, mycoplasma hominis, mycoplasma genitalium, and ureaplasma urealyticum infections on routine screen. a total of 714 subjects who had undergone cervical pap smears and concomitant analyses for cervical infections were included by a retrospective search. the frequencies of reactive cellular change (rcc) and squamous epithelial abnormalities were significantly higher in chlamydia positive subjects th ... | 2014 | 24526918 |
| prevalence of genital mycoplasmas in asymptomatic male partners of women diagnosed as having chlamydial infections. | we examined 209 asymptomatic male partners of women diagnosed as having chlamydial infections for the prevalence of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum, and ureaplasma parvum in their first-voided urine (fvu) by nucleic acid amplification tests. quantification of leukocytes in fvu was performed by automated urine particle analyzers. two (1.0%) men were positive for n. gonorrhoeae, and 92 (44.0%) were positive for c. trac ... | 2014 | 24486047 |
| prevalence of sexually transmitted infections among healthy korean women: implications of multiplex pcr pathogen detection on antibiotic therapy. | in this study, we investigated the prevalence of sexually transmitted infections (stis) using multiplex real-time pcr assay in healthy korean women. we also evaluated the risk factors of stis, and compared the various factors between the sti-positive and the sti-negative groups. a total of 799 endocervical swab samples from healthy korean women who visited our hospital for general medical check-ups during january 2012 to october 2012 were included. eight stis including human papillomavirus (hpv) ... | 2014 | 24462432 |
| co-infection of mycoplasma genitalium and chlamydia trachomatis in an infertile female patient with genital tuberculosis. | genital tuberculosis is a common cause of female infertility in india. but, it is important to screen for other agents like chlamydia trachomatis and genital mycoplasmas as well to avoid persistence of infection and its long-term sequelae. timely diagnosis of these infections using nucleic acid amplification tests and institution of appropriate therapy will improve the conception rates in infertile women. we report a case of co-infection of mycoplasma genitalium and chlamydia trachomatis in an i ... | 2014 | 24441248 |
| high prevalence of asymptomatic sexually transmitted infections among men who have sex with men. | men who have sex with men (msm) are disproportionately affected by sexually transmitted infection. the aim of this cross-sectional study is to prospectively detect the prevalence of chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), mycoplasma genitalium (mg), and high risk human papillomavirus (hr-hpv), and syphilis in a population of asymptomatic sexually active msm. | 2014 | 26237608 |
| [leading role of infection in formation of placental insufficiency]. | evaluate the role of endotoxinemia in pregnancy complicated with placental insufficiency. | 2013 | 24341208 |
| suboptimal adherence to doxycycline and treatment outcomes among men with non-gonococcal urethritis: a prospective cohort study. | doxycycline, one of two recommended therapies for non-gonococcal urethritis (ngu), consists of a 7-day course of therapy (100 mg bid). since suboptimal adherence may contribute to poor treatment outcomes, we examined the association between self-reported imperfect adherence to doxycycline and clinical and microbiologic failure among men with ngu. | 2014 | 24106340 |
| performance of anyplex™ ii multiplex real-time pcr for the diagnosis of seven sexually transmitted infections: comparison with currently available methods. | the real-time pcr assay is the most sensitive test for screening and diagnosing sexually transmitted infections (stis) and has made diagnosing these infections easier for clinicians. the aim of this study was to investigate the reliability, accuracy, and usefulness of the real-time multiplex pcr assay for the detection of seven sexually transmitted microorganisms in clinical samples. | 2013 | 24095619 |
| a survey on the prevalence of chlamydia trachomatis and mycoplasma genitalium infections in symptomatic and asymptomatic men referring to urology clinic of labbafinejad hospital, tehran, iran. | chlamydia trachomatis and mycoplasma genitalium infections are the most prevalent sexually transmitted bacterial infections in the world that cause urogenital infections in both men and women. it appears that infertility is a complication of these infections. | 2013 | 24083010 |
| molecular detection of potential sexually transmitted pathogens in semen and urine specimens of infertile and fertile males. | a total of 93 infertile and 70 fertile men attending various urology and gynecology clinics in jordan were investigated in this prospective study. first void urine and the corresponding semen specimens were collected from 96% of the patients. presence of neisseria gonorrhoeae (ng), chlamydia trachomatis (ct), ureaplasma urealyticum (uu), and mycoplasma genitalium (mg) dna in specimen was detected using polymerase chain reaction. the distribution of ng, ct, uu, and mg in semen and fvu specimens a ... | 2013 | 24079950 |
| asymptomatic mycoplasma genitalium infection amongst marginalised young people accessing a youth health service in melbourne. | we conducted a study to establish the prevalence of mycoplasma genitalium amongst asymptomatic young people experiencing homelessness in the context of comprehensive health care delivery. all asymptomatic young people at risk of sexually transmitted infections were offered urinary polymerase chain reaction testing for m. genitalium whilst being tested for chlamydia trachomatis and neisseria gonorrhoeae over a four-month period in 2012. those who tested positive were notified of the infection and ... | 2014 | 24026408 |
| prevalence, clinical features and quantification of genital non-viral infections. | we conducted a study of the prevalence, clinical features and microscopy findings, by retrospective case-notes survey, of six non-viral organisms, among 1718 attendees at a genitourinary (gu) medicine clinic in england. an in-house assay for six non-viral infections was used and quantitation of ureaplasmas performed. the prevalences of the six organisms were: chlamydia trachomatis (ct), 7.1%; neisseria gonorrhoeae (ng), 0.6%; mycoplasma genitalium (mg), 1.0%; trichomonas vaginalis, 0.2%; ureapla ... | 2013 | 23970658 |
| epidemiological trends of sexually transmitted infections among women in cheonan, south korea, 2006-2012. | a lack of investigation in specific regions has impeded the understanding of epidemiological trends in the prevalence of sexually transmitted infections (stis) in south korea. to help fill this research gap, this study used multiplex polymerase chain reaction (mpcr) to determine the prevalence of stis detected in clinical specimens collected from women in cheonan, south korea between august 2006 and november 2012, and analyzed the prevalence of stis according to age, bacterial pathogen, and time ... | 2013 | 23966022 |
| urethral inflammation in male chimpanzees caused by ureaplasmas and chlamydia trachomatis. | specimens from men with acute non-gonococcal urethritis were tested to determine their microbial content and then given intra-urethrally to male chimpanzees. two animals received ureaplasmas only and one became infected. the second did so when given a different strain. both developed a polymorphonuclear leukocyte (pmnl) response. two chimpanzees received a mixture of ureaplasmas and chlamydia trachomatis and there was a suggestion that the ureaplasmas delayed or suppressed the chlamydial respons ... | 2013 | 23904075 |
| bacterial infections of the lower genital tract in fertile and infertile women from the southeastern poland. | the objective of the study was to investigate the detection rates of chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, escherichia coli, streptococcus agalactiae and enterococcus faecalis, showing no clinical signs of an ongoing, acute inflammatory state of the vagina and/or the cenrvix, in fertile and infertile women. | 2013 | 23819400 |
| anatomic distribution of neisseria gonorrhoeae, chlamydia trachomatis and mycoplasma genitalium infections in men who have sex with men. | new cases of gonorrhoea (neisseria gonorrhoeae) and chlamydia (chlamydia trachomatis) infections have been steadily increasing in scandinavian countries over the last decade. there is a particular urgency in reducing new infections as isolation of multiple drug resistant strains of gonorrhoea is becoming more frequent. the aim of this study was to determine the prevalence and sites of infection of common sexually transmissible infections (stis) in men who have sex with men (msm). | 2013 | 23751932 |
| clinical efficacy of sitafloxacin 100 mg twice daily for 7 days for patients with non-gonococcal urethritis. | to clarify the clinical efficacy of stfx for patients with non-gonococcal urethritis (ngu), including chlamydial urethritis and mycoplasma genitalium-positive urethritis, this study included male patients with ngu who were 20 years old or older. the pathogens, including chlamydia trachomatis, m. genitalium and ureaplasma urealyticum, were detected by nucleic acid amplification tests and the patients were treated with sitafloxacin 100 mg twice daily for 7 days. microbiological and clinical effica ... | 2013 | 23749142 |
| cervicitis: limited clinical utility for the detection of mycoplasma genitalium in a cross-sectional study of women attending a new zealand sexual health clinic. | although multiple studies have confirmed mycoplasma genitalium as a cause of nongonococcal urethritis in men, there is less evidence of its pathogenicity in women. our aims were to determine the prevalence of m. genitalium in a sample of women attending a sexual health clinic and to assess whether there was any association between the detection of m. genitalium and a diagnosis of cervicitis in this population. | 2013 | 23702105 |
| association of sexually transmitted infections with high-risk human papillomavirus types: a survey with 802 female sex workers in china. | a cross-sectional study that investigated the association between cervical infection of chlamydia trachomatis, neisseria gonorrhoeae, or mycoplasma genitalium and infection with high-risk types of human papillomavirus among female sex workers in china found that the presence of cervical c. trachomatis or m. genitalium infection was independently associated with infection with certain high-risk types of specific high-risk human papillomavirus among this population. | 2013 | 23680906 |
| trends and associations of trichomonas vaginalis infection in men and women with genital discharge syndromes in johannesburg, south africa. | to better understand the epidemiology of trichomonas vaginalis infection, we investigated the association between t vaginalis and demographic, clinical, microbiological and behavioural characteristics of patients presenting with genital discharges to a primary healthcare clinic in johannesburg, south africa. | 2013 | 23605850 |
| mycoplasma genitalium in male urethritis: diagnosis and treatment in japan. | male urethritis is a common disease for urologists, with the most common pathogens being, chlamydia trachomatis and neisseria gonorrhoeae. when the tests fail to detect these pathogens, the presented urethritis is called non-chlamydial non-gonococcal urethritis. mycoplasma genitalium is one of the pathogens for non-chlamydial non-gonococcal urethritis. the test for detecting m. genitalium, which is commercially available in japan, is not accepted by the japanese insurance system now. the detecti ... | 2013 | 23586346 |
| mycoplasma genitalium: prevalence in men presenting with urethritis to a south australian public sexual health clinic. | this study aimed to determine the prevalence of mycoplasma genitalium infection among male patients with dysuria and/or urethral discharge. an analysis of the clinical, demographic and microbiological factors associated with m. genitalium infection was also conducted. | 2013 | 23425506 |
| the role of infectious agents in urogenital cancers. | since the late 1990s, infectious agents have been thought to play a role in the pathogenesis of approximately 15% of cancers. it is now widely accepted that infection of stomach tissue with the bacteria helicobacter pylori is an important cause of stomach adenocarcinoma. in addition, oncogenic viruses, such as papilloma viruses, herpes viruses, and hepadnaviruses are strongly associated with increased risk of cervical cancer, lymphomas, liver cancer, amongst others. however, in the scientific co ... | 2012 | 23198689 |
| chlamydia trachomatis, mycoplasma genitalium and ureaplasma urealyticum in clinical and non-clinical settings, arkhangelsk oblast, russia. | russia and eastern europe have the fastest growing hiv epidemic in the world. as sexually transmitted infections (stis) play an important role in hiv transmission, we conducted this study to find the prevalence of three microorganisms associated with stis in arkhangelsk oblast, russia. first void urine from 1729 participants was analysed using nucleic acid amplification testing, and all participants completed a questionnaire. one hundred and twelve (6.5%) were tested positive for chlamydia trach ... | 2012 | 23155097 |
| [microbiologic basis of diagnosis and treatment of pelvic inflammatory disease]. | pelvic inflammatory disease (pid) is caused by a large spectrum of micro-organisms. however, the microbiological cause is unknown in approximately half of cases according to varying series. in the context of sexually transmitted disease (std), the most frequently identified microorganisms causing pid are neisseria gonorrhoeae, chlamydia trachomatis and mycoplasma genitalium. in such cases, bacterial vaginosis and trichomonas vaginalis are frequently associated. in case of complicated pid or when ... | 2012 | 23140621 |
| prevalence and associations of genital ulcer and urethral pathogens in men presenting with genital ulcer syndrome to primary health care clinics in south africa. | this study aimed to determine the prevalence of genital ulcer and urethral pathogens, as well as their association with clinical features, in men with genital ulcer disease (gud) enrolled in a clinical trial. | 2012 | 23064538 |
| pelvic inflammatory disease (pid) from chlamydia trachomatis versus pid from neisseria gonorrhea: from clinical suspicion to therapy. | pelvic inflammatory disease (pid) is the most significant complication of sexually transmitted infections in childbearing-age women and it represents an important public health problem because of its long-term sequelae (chronic pelvic pain, tubal infertility, ectopic pregnancy). prior to the mid 1970s pid was considered a monoetiologic infection, due primarily to neisseria gonorrhea. now it is well documented as a polymicrobial process, with a great number of microrganisms involved. in addition ... | 2012 | 23007248 |
| hiv and sti control in el salvador: results from an integrated behavioural survey among men who have sex with men. | this cross-sectional study investigates hiv, other sexually transmitted infections (sti), and risk behaviours among men who have sex with men (msm) in two cities in el salvador. | 2012 | 22917694 |
| protocol for the detection of mycoplasma genitalium by pcr from clinical specimens and subsequent detection of macrolide resistance-mediating mutations in region v of the 23s rrna gene. | mycoplasma genitalium is an established cause of male nongonococcal urethritis, in particular in cases with recurrent disease and in those negative for chlamydia trachomatis. in women m. genitalium causes cervicitis and there is increasing evidence that it is causing pelvic inflammatory disease (pid). nucleic acid amplification tests are currently the only available methods for detection, but no commercially available tests have been thoroughly evaluated. here we describe a taqman-based pcr test ... | 2012 | 22782815 |
| mycoplasmateceae species are not found in fallopian tubes of women with tubo-peritoneal infertility. | the role of mycoplasmas on the development and sequelae of pelvic inflammatory disease remains controversial. the objective of the present study is to correlate directly the presence of mycoplasmateceae through polimerase chain reaction (pcr) determinations in cervix and fallopian tubes of infertile patients with tubo-peritoneal factor diagnosed through laparoscopy. | 2017 | 22729196 |
| urethritis/cervicitis pathogen prevalence and associated risk factors among asymptomatic hiv-infected patients in south africa. | to determine sexually transmitted infection (sti) prevalence, and patient characteristics associated with detection of urethritis/cervicitis pathogens, among hiv-infected individuals offered voluntary sti screening at a south african hiv treatment center. | 2012 | 22706215 |
| detection rates of mycoplasma genitalium and chlamydia trachomatis infections in novosibirsk, siberia, in 2010-2011. | | 2012 | 22661633 |
| [prevalence of chlamydia trachomatis, neisseria gonorrhoeae and mycoplasma genitalium infections in the emergency department]. | to estimate the prevalence of chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and mycoplasma genitalium (mg) in patients under 31 years of age admitted to the emergency department of the university hospital of montpellier, for which a urinalysis was performed. | 2013 | 22627011 |
| mycoplasma genitalium in cervicitis and pelvic inflammatory disease among women at a gynecologic outpatient service. | we sought to analyze the prevalence and clinical manifestations of mycoplasma genitalium infection in a heterogeneous population of women. | 2012 | 22483084 |
| chlamydia trachomatis, mycoplasma genitalium and ureaplasma urealyticum among students in northern norway. | the prevalence of mycoplasma genitalium and ureaplasma genitalium in populations outside sexually transmitted infection clinics in norway is unknown. | 2013 | 22449180 |
| the first performance report for the bio-rad dx ct/ng/mg assay for simultaneous detection of chlamydia trachomatis, neisseria gonorrhoeae and mycoplasma genitalium in urogenital samples. | we evaluated the clinical performance of the bio-rad dx ct/ng/mg assay for the detection of chlamydia trachomatis, mycoplasma genitalium and neisseria gonorrhoeae in urogenital samples in comparison with the roche cobas® taqman® ct assay for c. trachomatis and an in-house taqman pcr assay for m. genitalium. swab specimens were cultured for n. gonorrhoeae. in this prospective study, urogenital samples were obtained from symptomatic and asymptomatic patients attending the sexually transmitted dise ... | 2012 | 22446590 |
| sexual behaviours, knowledge and attitudes regarding safe sex, and prevalence of non-viral sexually transmitted infections among attendees of youth clinics in st. petersburg, russia. | adolescents and young adults are at increased risk of sexually transmitted infections (stis). knowledge of sti prevalence and risk factors are essential tools to elaborate preventive strategies. however, internationally reported studies on epidemiology of stis among the youth in russia are mainly lacking. | 2013 | 22429498 |
| clinical and microbiological outcomes in treatment of men with non-gonococcal urethritis with a 100-mg twice-daily dose regimen of sitafloxacin. | several microorganisms cause non-gonococcal urethritis (ngu). failure to eradicate mycoplasma genitalium from the urethra could be associated with persistent or recurrent urethritis; thus, the choice of antibiotics with activities potent enough to eradicate m. genitalium is crucial in the treatment of ngu. in in vitro studies, sitafloxacin has been shown to be highly active against chlamydia trachomatis and m. genitalium. we treated 89 males with ngu, including 15 patients with persistent or rec ... | 2012 | 22370921 |
| difficulties experienced in defining the microbial cause of pelvic inflammatory disease. | clinical assessment of women with pelvic pain was a poor indicator of disease seen at laparoscopy. thus, of 109 women, 22 at laparoscopy had salpingitis, 19 had adhesions without salpingitis, 20 had endometriosis or ovarian pathology and 48 no observable abnormality. in all laparoscopic categories, ureaplasma spp. and mycoplasma hominis, but not mycoplasma genitalium, were at least as common in the cervix/vagina as chlamydia trachomatis and equally frequent in the endometrium. however, c. tracho ... | 2012 | 22362682 |
| pelvic inflammatory disease: current concepts of diagnosis and management. | pelvic inflammatory disease (pid), one of the most common infections in non-pregnant women of reproductive age, remains an important public health problem. it is associated with major long-term sequelae, including tubal factor infertility, ectopic pregnancy, and chronic pelvic pain. in addition, treatment of acute pid and its complications incurs substantial health care costs. prevention of these long-term sequelae is dependent upon clinicians having a high index of suspicion in order to make an ... | 2012 | 22298157 |
| evaluation of seeplex® std6 ace detection kit for the diagnosis of six bacterial sexually transmitted infections. | traditionally, the diagnosis of bacterial sexually transmitted infection (sti) has been dependent on the isolation of the causative pathogens by culturing endocervical or urethral swab specimens on selective media. while such procedures typically provide excellent diagnostic accuracy, they are often time-consuming and expensive. a multiplex polymerase chain reaction (pcr) assay, based on a semi-automated detection system, was evaluated for the detection of six sti causative organisms. the seeple ... | 2012 | 22252268 |
| supersize me: cronobacter sakazakii phage gap32. | cronobacter sakazakii is a gram-negative pathogen found in milk-based formulae that causes infant meningitis. bacteriophages have been proposed to control bacterial pathogens; however, comprehensive knowledge about a phage is required to ensure its safety before clinical application. we have characterized c. sakazakii phage vb_csam_gap32 (gap32), which possesses the second largest sequenced phage genome (358,663bp). a total of 571 genes including 545 protein coding sequences and 26 trnas were id ... | 2014 | 25010279 |
| synthetic genome engineering forging new frontiers for wine yeast. | over the past 15 years, the seismic shifts caused by the convergence of biomolecular, chemical, physical, mathematical, and computational sciences alongside cutting-edge developments in information technology and engineering have erupted into a new field of scientific endeavor dubbed synthetic biology. recent rapid advances in high-throughput dna sequencing and dna synthesis techniques are enabling the design and construction of new biological parts (genes), devices (gene networks) and modules ( ... | 2017 | 27535766 |
| correction: improved metabolic models for e. coli and mycoplasma genitalium from globalfit, an algorithm that simultaneously matches growth and non-growth data sets. | [this corrects the article doi: 10.1371/journal.pcbi.1005036.]. | 2016 | 27935945 |
| improved metabolic models for e. coli and mycoplasma genitalium from globalfit, an algorithm that simultaneously matches growth and non-growth data sets. | constraint-based metabolic modeling methods such as flux balance analysis (fba) are routinely used to predict the effects of genetic changes and to design strains with desired metabolic properties. the major bottleneck in modeling genome-scale metabolic systems is the establishment and manual curation of reliable stoichiometric models. initial reconstructions are typically refined through comparisons to experimental growth data from gene knockouts or nutrient environments. existing methods itera ... | 2016 | 27482704 |
| identification of macrolide-resistant mycoplasma genitalium using real-time pcr. | mycoplasma genitalium is a common cause of non-gonococcal urethritis (ngu) in western europe, but is not routinely tested for in all clinics. a high prevalence of macrolide-resistant m. genitalium has been reported. an easy to use test that can predict likely macrolide treatment failure is potentially very valuable. we report the development of a rapid and reliable real-time pcr-assay which detects all relevant resistance loci in the m. genitalium 23s rrna gene. | 2015 | 25622510 |
| introduction to focus issue: quantitative approaches to genetic networks. | all cells of living organisms contain similar genetic instructions encoded in the organism's dna. in any particular cell, the control of the expression of each different gene is regulated, in part, by binding of molecular complexes to specific regions of the dna. the molecular complexes are composed of protein molecules, called transcription factors, combined with various other molecules such as hormones and drugs. since transcription factors are coded by genes, cellular function is partially de ... | 2013 | 23822498 |
| a serine/threonine phosphatase encoded by mg_207 of mycoplasma genitalium is critical for its virulence. | bacterial signal transduction systems like two component system (tcs) and serine/threonine kinase (stk) and serine/threonine phosphatase (stp) play important roles in the virulence and pathogenesis of bacterial pathogens. mycoplasma genitalium, a mollicute that causes the urogenital diseases urethritis and cervicitis in men and women, respectively, is a pathogen which lacks tcs but possesses stk/stp. in this study, we investigated the biochemical and virulence properties of an stp protein encode ... | 2013 | 23432936 |
| detection of macrolide resistance in mycoplasma genitalium in france. | mycoplasma genitalium is a sexually transmitted organism associated with non-gonococcal urethritis in men and several inflammatory reproductive tract syndromes in women. resistance to macrolides has been recently associated with point mutations in the 23s rrna gene. the aim of this study was to detect these mutations using a large french collection of m. genitalium-positive specimens. we evaluated whether these mutations were related to azithromycin treatment failure and whether macrolide-resist ... | 2012 | 22773740 |