| search for the presence of six mycoplasma species in peripheral blood mononuclear cells of subjects seropositive and seronegative for human immunodeficiency virus. | the prevalence of mycoplasma fermentans, mycoplasma pirum, mycoplasma genitalium, mycoplasma pneumoniae, mycoplasma hominis, and mycoplasma penetrans was investigated by using specific pcr assays with peripheral blood mononuclear cells from subjects infected or not infected with the human immunodeficiency virus (hiv). only m. fermentans was detected in 5.8% of 154 hiv-seropositive and 11.1% of 90 hiv-seronegative subjects. | 1996 | 8784596 |
| isolation of mycoplasma genitalium strains from the male urethra. | mycoplasma genitalium is a human mycoplasma species which, on the basis of detection by pcr, has been incriminated as a cause of nongonococcal urethritis. previously, only two strains from the urogenital tract and five strains from extragenital sites have been isolated. we have developed a method for the isolation of this fastidious microbe. m. genitalium from pcr-positive urethral specimens was initially propagated in vero cell cultures grown in serum-free medium supplemented with ultroser hy s ... | 1996 | 8789002 |
| novelties from the complete genome of mycoplasma genitalium. | | 1996 | 8793887 |
| a minimal gene set for cellular life derived by comparison of complete bacterial genomes. | the recently sequenced genome of the parasitic bacterium mycoplasma genitalium contains only 468 identified protein-coding genes that have been dubbed a minimal gene complement [fraser, c.m., gocayne, j.d., white, o., adams, m.d., clayton, r.a., et al. (1995) science 270, 397-403]. although the m. genitalium gene complement is indeed the smallest among known cellular life forms, there is no evidence that it is the minimal self-sufficient gene set. to derive such a set, we compared the 468 predic ... | 1996 | 8816789 |
| detection of mycoplasma genitalium from urethral swabs of human immunodeficiency virus-infected patients. | mycoplasma have been suggested as co-factors in the pathogenesis of acquired immune deficiency syndrome (aids). the prevalence of urethral infection by mycoplasma genitalium was determined by polymerase chain reaction (pcr) with urethral swabs from 35 hiv-infected patients at different stages of the disease (all of them were heterosexual men). m genitalium was detected in 2 out of 19 non-aids (stage a and b) patients and in a similar proportion (1 out of 14; 7.1%) of samples from healthy individ ... | 1996 | 8841035 |
| sequence and organization of genes encoding enzymes involved in pyruvate metabolism in mycoplasma capricolum. | the region of the genome of mycoplasma capricolum upstream of the portion encompassing the genes for enzymes i and iiaglc of the phosphoenolpyruvate:sugar phosphotransferase system (pts) was cloned and sequenced. examination of the sequence revealed open reading frames corresponding to numerous genes involved with the oxidation of pyruvate. the deduced gene organization is naox (encoding nadh oxidase)-lpla (encoding lipoate-protein ligase)-odpa (encoding pyruvate dehydrogenase ei alpha)-odpb (en ... | 1996 | 8844861 |
| identification of new rna modifying enzymes by iterative genome search using known modifying enzymes as probes. | the complete nucleotide sequences of the haemophilus influenzae and mycoplasma genitalium genomes and the partially sequenced escherichia coli chromosome were analyzed to identify open reading frames (orfs) likely to encode rna modifying enzymes. the protein sequences of known rna modifying enzymes from three families--m5u methyltransferases, psi synthases and 2'-o methyltransferases--were used as probes to search sequence databases for homologs. orfs identified as homologous to the initial prob ... | 1996 | 8871555 |
| interplay between mycoplasma surface proteins, airway cells, and the protean manifestations of mycoplasma-mediated human infections. | adherence of mycoplasmas to specific tissue surfaces is a crucial step in the establishment of infection. several pathogenic mycoplasmas are flask-shaped and possess specialized tips that permit a highly oriented surface parasitism of host target cells. mycoplasma pneumoniae, which causes primary atypical pneumonia in humans, requires a network of interactive adhesins and accessory proteins to cytadhere. the adhesins must cluster at the mycoplasma tip organelle in close association with cytadher ... | 1996 | 8876532 |
| a simple vectorial representation of dna sequences for the detection of replication origins in bacteria. | a simple adaptation of ninio's vectorial representation of dna sequences for the detection of replication origins in bacteria is presented. the origins of replication in escherichia coli, bacillus subtilis, haemophilus influenzae and mycoplasma genitalium are well outlined with this graphical representation. | 1996 | 8905151 |
| isolation and characterization of transposon tn4001-generated, cytadherence-deficient transformants of mycoplasma pneumoniae and mycoplasma genitalium. | cytadherence and subsequent parasitism of host cells by the human pathogens, mycoplasma pneumoniae and mycoplasma genitalium, are mediated by adhesins and adherence-related accessory proteins. in this report we demonstrate the use of transposon tn4001 to generate tn-induced transformants displaying cytadherence-deficient characteristics. mycoplasma pneumoniae tn-generated transformant, designated 8r, lacked the high-molecular weight adherence-accessory proteins hmw1/4 and was deficient in hemads ... | 1996 | 8908481 |
| in the search of mycoplasma genitalium lost substrate-binding proteins: sequence divergence could be the result of a broader substrate specificity. | | 1996 | 8930922 |
| prokaryotic dna sequences in patients with chronic idiopathic prostatitis. | half of all men experience symptoms of prostatitis at some time in their lives, but the etiology is unknown for more than 90% of patients. optimal clinical and culture methods were used to select 135 men with chronic prostatitis refractory to multiple previous courses of antimicrobial therapy. the subjects had no evidence of structural or functional lower genitourinary tract abnormalities of bacteriuria or bacterial prostatitis by traditional clinical tests, or of urethritis or urethral pathogen ... | 1996 | 8940458 |
| prevalence of mycoplasma genitalium in men with gonococcal urethritis. | a significant association of mycoplasma genitalium with non-gonococcal urethritis has been reported, but the prevalence of this mycoplasma in men with gonococcal urethritis has not been so well studied. in this study, we examined urethral swab specimens from 45 japanese male patients with gonococcal urethritis for the presence of m. genitalium by using a polymerase chain reaction-based assay. we also sought chlamydia trachomatis by an enzyme immunoassay (chlamydiazyme). of the 45 specimens, 2 (4 ... | 1996 | 8940675 |
| identification and sequences of the treponema pallidum mgla and mglc genes. | treponema pallidum, the agent of syphilis, cannot be continuously cultivated in vitro. to identify treponemal genes encoding exported proteins, we performed tnphoa mutagenesis of a t. pallidum genomic dna library in escherichia coli. clone 6d2 was chosen for further study based on partial nucleotide sequence obtained from p6d2 containing a tnphoa insertion. a complete open reading frame (orf1) and a truncated orf (orf2) were identified in the treponemal dna of p6d2. orf1 encodes a hydrophobic pr ... | 1996 | 8988365 |
| complete genome sequences of cellular life forms: glimpses of theoretical evolutionary genomics. | the availability of complete genome sequences of cellular life forms creates the opportunity to explore the functional content of the genomes and evolutionary relationships between them at a new qualitative level. with the advent of these sequences, the construction of a minimal gene set sufficient for sustaining cellular life and reconstruction of the genome of the last common ancestor of bacteria, eukaryotes, and archaea become realistic, albeit challenging, research projects. a version of the ... | 1996 | 8994848 |
| phylogenetic analysis of mycoplasmas based on hsp70 sequences: cloning of the dnak (hsp70) gene region of mycoplasma capricolum. | a 5.0-kb region containing the hsp70 (dnak) gene was cloned from mycoplasma capricolum and sequenced. in addition to the hsp70 gene, this sequence region also contained the complete sequences for the grpe and orfa genes and partial sequences for the clpb and dnaj genes. the order of the above gene sequences in the cloned fragment was found to be clpb-orfa-grpe-hsp70-dnaj, which is similar to the order seen in various other gram-positive groups of bacteria. the hsp70 homologs from two mycoplasma ... | 1997 | 8995799 |
| characterization of the gene for an immunodominant 72 kda lipoprotein of mycoplasma mycoides subsp. mycoides small colony type. | with the aim of characterizing specific immunogenic proteins of mycoplasma mycoides subsp. mycoides small colony (sc) type, the aetiological agent of contagious bovine pleuropneumonia, a gene encoding a major immunogenic protein of 72 kda named p72 was cloned and expressed in escherichia coli. the expressed protein was of the same apparent molecular mass as that produced by the parent strain. the predicted molecular mass of p72, based on the dna-deduced amino acid sequence, was 61.118 kda, signi ... | 1996 | 9004514 |
| the metabolic pathway collection: an update. | the metabolic pathway collection from emp is an extraction of data from the larger enzymes and metabolic pathways database (emp). this extraction has been made publicly available in the hope that others will find it useful for a variety of purposes. the original release in october 1995 contained 1814 distinct pathways. the current collection contains 2180. metabolic reconstructions for the first completely sequenced organisms-haemophilus influenzae,mycoplasma genitalium,saccharomyces cerevisiaea ... | 1997 | 9016500 |
| the translational signal database, transterm: more organisms, complete genomes. | transterm is a database of initiation and termination sequence contexts from more than 250 organisms listed in genbank, including the four complete genomes:haemophilus influenzae, methanococcus jannaschii, mycoplasma genitalium,and saccharomyces cerevisiae. for the current release, more than 60 000 coding sequences were analysed. the tabulated data include initiation and termination contexts organised by species along with quantitative parameters about individual coding sequences (length, %gc, g ... | 1997 | 9016547 |
| comparative analysis of the genomes of the bacteria mycoplasma pneumoniae and mycoplasma genitalium. | the sequenced genomes of the two closely related bacteria mycoplasma genitalium and mycoplasma pneumoniae were compared with emphasis on genome organization and coding capacity. all the 470 proposed open reading frames (orfs) of the smaller m.genitalium genome (580 kb) were contained in the larger genome (816 kb) of m.pneumoniae. there were some discrepancies in annotation, but inspection of the dna sequences showed that the corresponding dna was always present in m. pneumoniae. the two genomes ... | 1997 | 9016618 |
| correspondence discriminant analysis: a multivariate method for comparing classes of protein and nucleic acid sequences. | this report describes two applications of a multivariate method for studying classes of nucleotide or protein sequences: correspondence discriminant analysis (cda). the first example is the discrimination between escherichia coli proteins according to their subcellular location (membrane, cytoplasm and periplasm). the high resolution of the method made it possible to predict the subcellular location of e.coli proteins for whom this information is not known. the second example is discrimination b ... | 1996 | 9021271 |
| a bacillus cereus member of the snf2 family. | the complete sequence of a bacillus cereus member of the snf2 family of putative helicases showed conservation of all seven motifs typical of this family. bcsnf predicted a protein of 1064 aa where the conserved snf2 domain was located at the carboxy terminus, whereas the 633 amino-terminal aa showed no homology to any protein in the databases. a putative transcriptional start was identified by primer extension, indicating that bcsnf is not a part of a larger operon. no phenotypical changes were ... | 1997 | 9025290 |
| a survey of polypeptide deformylase function throughout the eubacterial lineage. | n-terminal formylation of ribosome-synthesized polypeptides is assumed to be among the most conserved features that distinguish the eubacterial line of descent from other living phyla. in order to assess the ancientness of this trait, def genes encoding polypeptide deformylase were characterized from four eubacterial species, lactococcus lactis, bacillus subtilis, calothrix pcc7601 and thermotoga maritima, taking advantage of the conditional viability of the def mutants of escherichia coli. alto ... | 1997 | 9086272 |
| molecular cloning and analysis of a hsp (heat shock protein)-like 42 kda antigen gene of mycoplasma hyopneumoniae. | the recombinant clone expressing the 42 kda protein (p42) of mycoplasma hyopneumoniae in escherichia coli was analyzed. the 4.4 kb hindiii-xmal dna fragment expressing the p42 gene product encodes three orfs: p42 and p16 in the forwarding strand, p24 in the reverse strand. sequence comparisons revealed that p42 could be part of a p65 gene, and has 62% identities with mycoplasma genitalium hsp70 gene and 56% identities with bacillus subtilis dnak gene; p16 and p24 genes share 73% and 47% identiti ... | 1997 | 9111943 |
| structure-function relationships within the peptide deformylase family. evidence for a conserved architecture of the active site involving three conserved motifs and a metal ion. | thermus thermophilus peptide deformylase was characterized. its enzymatic properties as well as its organization in domains proved to share close resemblances with those of the escherichia coli enzyme despite few sequence identities. in addition to the hexxh signature sequence of the zinc metalloprotease family, a second short stretch of strictly conserved amino acids was noticed, egcls, the cysteine of which corresponds to the third zinc ligand. the study of site-directed mutants of the e. coli ... | 1997 | 9126850 |
| prevalence of mycoplasma genitalium in asymptomatic men in japan. | we aim to clarify the prevalence of mycoplasma genitalium in asymptomatic men in japan. first-catch urine specimens were obtained from 187 asymptomatic japanese men and examined for the presence of m. genitalium using a polymerase chain reaction (pcr)-based assay. two (1.1%) of 187 first-catch urine specimens were positive for m. genitalium. the prevalence of m. genitalium in urine specimens of asymptomatic men in japan is lower than that in asymptomatic men in the uk (6%) and denmark (9%). | 1997 | 9147160 |
| probing protein function using a combination of gene knockout and proteome analysis by mass spectrometry. | recently the determination of the genome sequences of three procaryotes (haemophilus influenzae, methanococcus jannaschii and mycoplasma genitalium) as well as the first eucaryotic genome (saccharomyces cerevisiae) were completed. between 40-60% of the genes were found to code for proteins to which no function could be assigned. we describe an approach which combines proteome analysis (mapping of expressed proteins isolated by two-dimensional polyacrylamide gel electrophoresis to the genome) wit ... | 1997 | 9150922 |
| mycoplasma genitalium in the cervices of japanese women. | mycoplasma genitalium is considered a cause of nongonococcal urethritis in men. this organism also is a cause of genital infections in women, and has been detected in women attending sexually transmitted disease clinics in the united kingdom and denmark, although its prevalence is unknown in japanese women. | 1997 | 9153738 |
| avoidance of palindromic words in bacterial and archaeal genomes: a close connection with restriction enzymes. | short palindromic sequences (4, 5 and 6 bp palindromes) are avoided at a statistically significant level in the genomes of several bacteria, including the completely sequenced haemophilus influenzae and synechocystis sp. genomes and in the complete genome of the archaeon methanococcus jannaschii. in contrast, there is only moderate avoidance of palindromes in the small genome of the bacterium mycoplasma genitalium and no detectable avoidance in the genomes of chloroplasts and mitochondria. the s ... | 1997 | 9171096 |
| expression, purification, and characterization of enzyme iia(glc) of the phosphoenolpyruvate:sugar phosphotransferase system of mycoplasma capricolum. | the gene encoding enzyme iia(glc) (eiia) of the phosphoenolpyruvate:sugar phosphotransferase system of mycoplasma capricolum was cloned into a regulated expression vector. the purified protein product of the overexpressed gene was characterized as an active phosphoacceptor from hpr with a higher pi than previously described eiias. m. capricolum eiia was unreactive with antibodies directed against the corresponding proteins from either gram-positive or gram-negative bacteria. enzyme iia(glc) beha ... | 1997 | 9188690 |
| compositional biases of bacterial genomes and evolutionary implications. | we compare and contrast genome-wide compositional biases and distributions of short oligonucleotides across 15 diverse prokaryotes that have substantial genomic sequence collections. these include seven complete genomes (escherichia coli, haemophilus influenzae, mycoplasma genitalium, mycoplasma pneumoniae, synechocystis sp. strain pcc6803, methanococcus jannaschii, and pyrobaculum aerophilum). a key observation concerns the constancy of the dinucleotide relative abundance profiles over multiple ... | 1997 | 9190805 |
| nucleotide sequence and analysis of the phob-rrne-groesl region of the bacillus subtilis chromosome. | a 36 kb sequence of the phob-rrne-groesl region of the bacillus subtilis chromosome at around 55 degrees has been determined. the sequenced region contains 36 orfs including the phob and groesl genes, and the whole rrne operon. the phob gene is transcribed in the direction opposite to that of chromosome replication, while most orfs, including groesl and the rrne operon, are transcribed in the same direction. two newly identified trna genes upstream of the rrne operon were those for arg-trna and ... | 1997 | 9202461 |
| mycoplasma genitalium in male urethritis. | | 1997 | 9259507 |
| microbiology of semen specimens from males attending a fertility clinic. | the relationship between semen quality, pyospermia and bacteriology was studied in 201 semen specimens from male patients attending a fertility clinic. semen quality parameters were within normal limits in 115 (57%) patients, slightly reduced in 60 (30%), and 26 (13%) had findings indicating reduced fertility. twelve patients (6%) had pyospermia. in 182 patients, 552 microorganisms were detected, including enterobacteriaceae (2.8%), gardnerella vaginalis (9.6%), chlamydia trachomatis (1.6%), myc ... | 1997 | 9269303 |
| analysis of the bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnb) and 284 degrees (pai). | in the framework of the international project aimed at the sequencing of the bacillus subtilis genome, five dna fragments in the region between rrnb (275 degrees) and pai (284 degrees) were cloned by inverse and combinatorial long-range pcr and their nucleotide sequences were determined and analysed. together these sequences constituted a contig of 62229 bp. on the basis of the position of not1 and stil restriction sites, the orientation and order of known genetic markers was determined to be pa ... | 1997 | 9274030 |
| comparative genomics of mycoplasmas. | the mycoplasmas are the smallest and simplest self-replicating organisms. the goal of defining in molecular terms the entire machinery of a living cell by using mycoplasmas as models was put forward by harold morowitz in 1984. the recent complete sequencing of the genomes of the human pathogens mycoplasma genitalium and mycoplasma pneumoniae brings us much closer to achieving this goal. the m. genitalium genome contains only 479 predicted protein coding sequences (genes) and that of m. pneumonia ... | 1997 | 9286058 |
| molecular biology of mycoplasma. | mycoplasmas are the smallest free living microorganisms with the smallest genome. the g+c content is in general low (25-33%) and the coding capacity is about 600 proteins. mycoplasma species are phylogenetically related, they use the genetic codon uga for tryptophan, and show rapid evolution, with a high rate of divergence. the genomes of mycoplasma genitalium and mycoplasma pneumoniae have been fully sequenced. striking features of the m. genitalium sequencing project are the presence of a high ... | 1997 | 9286059 |
| proteome analysis of spiroplasma melliferum (a56) and protein characterisation across species boundaries. | spiroplasma melliferum (class: mollicutes) is a wall-less, helical bacterium with a genome of approximately 1460 kbp encoding 800-1000 gene-products. a two-dimensional electrophoresis gel reference map of s. melliferum was produced by phoretix 2-d gel software analysis of eight high quality gels. the reference map showed 456 silver-stained and replicated protein spots. 156 proteins (34% of visible protein spots) from s. melliferum were further characterised by one, or a combination, of the follo ... | 1997 | 9298648 |
| evaluation of algorithms used for cross-species proteome characterisation. | the ability to effectively search databases for the identification of protein spots from two-dimensional electrophoresis gels has become an essential step in the study of microbial proteomes. a variety of analytical techniques are currently being employed during protein characterisation. a number of algorithms used to search databases, accessible via the world wide web, depend upon information concerning n- and c-terminal microsequence, amino acid composition, and peptide-mass fingerprinting. th ... | 1997 | 9298655 |
| malate/lactate dehydrogenase in mollicutes: evidence for a multienzyme protein. | the malate (mdh) and lactate (ldh) dehydrogenases belong to the homologous class of 2-ketoacid dehydrogenases. the specificity for their respective substrates depends on residues differing at two or three regions within each molecule. theoretical peptide-mass fingerprinting and prosite analysis of nine mdh and six ldh molecules were used to describe conserved sites related to function. a unique ldh is described which probably also confers mdh activity within the 580 kbp genome of mycoplasma geni ... | 1997 | 9305754 |
| characterization of the rpoc gene of streptomyces coelicolor a3(2) and its use to develop a simple and rapid method for the purification of rna polymerase. | the streptomyces coelicolor rpoc gene, that encodes the beta' subunit of rna polymerase, was isolated using the escherichia coli rpoc gene as a hybridization probe. comparison of the predicted amino acid sequence of the s. coelicolor beta' subunit to those characterized from other bacteria revealed three distinct subfamilies of beta' subunits, one of which consists of the s. coelicolor subunit and those from mycobacterium leprae and mycoplasma genitalium. using site-directed mutagenesis, the car ... | 1997 | 9322738 |
| a comparative study of duplications in bacteria and eukaryotes: the importance of telomeres. | the genomes of three bacteria (haemophilus influenzae, mycoplasma genitalium, and escherichia coli) and two eukaryotes (saccharomyces cerevisiae and caenorhabditis elegans) were compared. the distribution of their putative open reading frames (orfs) was studied, and several conclusions were drawn: (1) all of these genomes, even the smallest, exhibit a significant proportion (7%-30%) of duplicated orfs. this proportion is a function of genome size and appears unrelated to the bacteria/eukaryote d ... | 1997 | 9335146 |
| assigning folds to the proteins encoded by the genome of mycoplasma genitalium. | a crucial step in exploiting the information inherent in genome sequences is to assign to each protein sequence its three-dimensional fold and biological function. here we describe fold assignment for the proteins encoded by the small genome of mycoplasma genitalium. the assignment was carried out by our computer server (http://www.doe-mbi.ucla.edu/people/frsvr/ frsvr. html), which assigns folds to amino acid sequences by comparing sequence-derived predictions with known structures. of the total ... | 1997 | 9342339 |
| conserved gene clusters in bacterial genomes provide further support for the primacy of rna. | five complete bacterial genome sequences have been released to the scientific community. these include four (eu)bacteria, haemophilus influenzae, mycoplasma genitalium, m. pneumoniae, and synechocystis pcc 6803, as well as one archaeon, methanococcus jannaschii. features of organization shared by these genomes are likely to have arisen very early in the history of the bacteria and thus can be expected to provide further insight into the nature of early ancestors. results of a genome comparison o ... | 1997 | 9342394 |
| the minimal cellular genome of mycoplasma. | the mycoplasmas are the smallest and simplest self-replicating organisms, being built of a plasma membrane, ribosomes, and a circular double-stranded dna molecule-the typical prokaryotic genome. the idea of using mycoplasmas as models for defining in molecular terms the entire machinery of a living cell was raised by morowitz in 1984. the goal has been to prove the dogma of the completeness of molecular biology, that is, that the logic of life is finite, relatively simple and subject to full exp ... | 1997 | 9343940 |
| systematic base composition variation around the genome of mycoplasma genitalium, but not mycoplasma pneumoniae. | | 1997 | 9350873 |
| the immunoreactive 116 kda surface protein of mycoplasma pneumoniae is encoded in an operon. | sera from 10 patients infected with mycoplasma pneumoniae were used in western blot analysis of triton-x-114-soluble protein preparations of m. pneumoniae. all 10 sera were reactive with a protein antigen of 116 kda. sera from another 17 patients were used in western blot analysis of whole-cell m. pneumoniae proteins; 15 of these sera were reactive with the 116 kda protein. trypsin digestion of whole m. pneumoniae cells demonstrated the surface location of this protein. sequencing of dna which c ... | 1997 | 9353941 |
| detection of urogenital mycoplasmal infections in primates by use of polymerase chain reaction. | urogenital mycoplasmal infections could affect use of primates as models for reproductive system studies and could affect reproduction in captive primates, but could be useful as animal models of similar human infections. we conducted a pilot study to assess detection of urogenital mycoplasmal infections in primates by use of polymerase chain reaction (pcr). healthy animals were anesthetized, and vaginal, cervical, or endometrial and urethral swab specimens were collected from females and males, ... | 1997 | 9355087 |
| analysis of 74 kb of dna located at the right end of the 330-kb chlorella virus pbcv-1 genome. | this report completes a preliminary analysis of the sequence of the 330,740-bp chlorella virus pbcv-1 genome, the largest virus genome to be sequenced to date. the pbcv-1 genome is 57% the size of the genome from the smallest self-replicating organism, mycoplasma genitalium. analysis of 74 kb of newly sequenced dna, from the right terminus of the pbcv-1 genome, revealed 153 open reading frames (orfs) of 65 codons or longer. eighty-five of these orfs, which are evenly distributed on both strands ... | 1997 | 9356347 |
| comparison of archaeal and bacterial genomes: computer analysis of protein sequences predicts novel functions and suggests a chimeric origin for the archaea. | protein sequences encoded in three complete bacterial genomes, those of haemophilus influenzae, mycoplasma genitalium and synechocystis sp., and the first available archaeal genome sequence, that of methanococcus jannaschii, were analysed using the blast2 algorithm and methods for amino acid motif detection. between 75% and 90% of the predicted proteins encoded in each of the bacterial genomes and 73% of the m. jannaschii proteins showed significant sequence similarity to proteins from other spe ... | 1997 | 9379893 |
| detection of mycoplasma genitalium and chlamydia trachomatis dnas in male patients with urethritis using the polymerase chain reaction. | the use of pcr assays as a fast and reliable method is constantly improving and easing microbiological diagnosis. we used a polymerase chain reaction (pcr) assay designed to detect mycoplasma genitalium and chlamydia trachomatis in urethral swab samples of 56 males with urethritis and 44 asymptomatic patients as a control group. the pcr assay provides an amplification of target sequence within mgpa (m. genitalium protein attachment) gene. results indicated that m. genitalium was present in 6 (10 ... | 1997 | 9385602 |
| markers of sexually transmitted diseases in seminal fluid of male clients of female sex workers. | to screen for certain std markers in a group of male clients of female sex workers. | 1997 | 9389951 |
| genome plasticity as a paradigm of eubacteria evolution. | to test the hypotheses that eubacterial genomes leave evolutionarily stable structures and that the variety of genome size is brought about through genome doubling during evolution, the genome structures of haemophilus influenzae, mycoplasma genitalium, escherichia coli, and bacillus subtilis were compared using the dna sequences of the entire genome or substantial portions of genome. in these comparisons, the locations of orthologous genes were examined among different genomes. using orthologou ... | 1997 | 9395406 |
| a 71-kda protein from halobacterium salinarium belongs to a ubiquitous p-loop atpase superfamily with head-rod-tail structure. | the nucleotide sequence of a genomic fragment from halobacterium salinarium containing an open reading frame encoding a protein with a calculated molecular mass of 71 kda was determined. database searches revealed that this protein, hp71, has similarities to eukaryotic cytoskeletal proteins. heterologous production of hp71 in escherichia coli allowed the isolation of anti-hp71 antibodies. the antibodies were used (1) to verify the production of hp71 in h. salinarium and (2) to determine its cyto ... | 1998 | 9396829 |
| comparison of dna sequences with protein sequences. | the fasta package of sequence comparison programs has been expanded to include fastx and fasty, which compare a dna sequence to a protein sequence database, translating the dna sequence in three frames and aligning the translated dna sequence to each sequence in the protein database, allowing gaps and frameshifts. also new are tfastx and tfasty, which compare a protein sequence to a dna sequence database, translating each sequence in the dna database in six frames and scoring alignments with gap ... | 1997 | 9403055 |
| genomic sequence of a lyme disease spirochaete, borrelia burgdorferi. | the genome of the bacterium borrelia burgdorferi b31, the aetiologic agent of lyme disease, contains a linear chromosome of 910,725 base pairs and at least 17 linear and circular plasmids with a combined size of more than 533,000 base pairs. the chromosome contains 853 genes encoding a basic set of proteins for dna replication, transcription, translation, solute transport and energy metabolism, but, like mycoplasma genitalium, it contains no genes for cellular biosynthetic reactions. because b. ... | 1997 | 9403685 |
| antibiotic susceptibilities of mycoplasmas and treatment of mycoplasmal infections. | mycoplasmas are the smallest free-living microorganisms, being about 300 nm in diameter. they are bounded by a triple-layered membrane and, unlike conventional bacteria, do not have a rigid cell wall. hence, they are not susceptible to penicillins and other antibiotics that act on this structure. they are, however, susceptible to a variety of other broad-spectrum antibiotics, most of which only inhibit their multiplication and do not kill them. the tetracyclines have always been in the forefront ... | 1997 | 9421309 |
| detection and confirmation of mycoplasma pneumoniae in urogenital specimens by pcr. | following the isolation of mycoplasma pneumoniae from urogenital specimens (m. goulet, r. dular, j. g. tully, g. billows, and s. kasatiya, j. clin. microbiol. 33:2823-2825, 1995), a study was undertaken to confirm the observations by pcr. specific primers directed to the p1 adhesin gene of m. pneumoniae were used. a total of 300 genital specimens were tested for m. pneumoniae and mycoplasma genitalium by culture and pcr. of these, 15 were positive by culture and 17 were positive by pcr for m. pn ... | 1998 | 9431966 |
| the comparative metabolism of the mollicutes (mycoplasmas): the utility for taxonomic classification and the relationship of putative gene annotation and phylogeny to enzymatic function in the smallest free-living cells. | mollicutes or mycoplasmas are a class of wall-less bacteria descended from low g + c% gram-positive bacteria. some are exceedingly small, about 0.2 micron in diameter, and are examples of the smallest free-living cells known. their genomes are equally small; the smallest in mycoplasma genitalium is sequenced and is 0.58 mb with 475 orfs, compared with 4.639 mb and 4288 orfs for escherichia coli. because of their size and apparently limited metabolic potential, mollicutes are models for describin ... | 1997 | 9439886 |
| mycoplasma genitalium infection and host antibody immune response in patients infected by hiv, patients attending std clinics and in healthy blood donors. | prevalence of mycoplasma genitalium in humans is still not clear. we have developed a sensitive and specific serological assay for m. genitalium using lipid-associated membrane proteins (lamps) as antigens. antibodies to lamps from m. genitalium showed little cross-reactivity to lamps from antigenically similar m. pneumoniae. for validity testing, urines from 104 patients were tested by pcr for m. genitalium. all 15 pcr+ patients had m. genitalium-lamps antibodies. moreover, none of 64 antibody- ... | 1997 | 9453394 |
| development and evaluation of a pcr-based assay for detection of haemobartonella felis in cats and differentiation of h. felis from related bacteria by restriction fragment length polymorphism analysis. | the 16s rrna gene of haemobartonella felis was amplified by using universal eubacterial primers and was subsequently cloned and sequenced. based on this sequence data, we designed a set of h. felis-specific primers. these primers selectively amplified a 1,316-bp dna fragment of the 16s rrna gene of h. felis from each of four experimentally infected cats at peak parasitemia. no pcr product was amplified from purified dna of eperythrozoon suis, mycoplasma genitalium, and bartonella bacilliformis. ... | 1998 | 9466759 |
| a review of the in-vitro activity of quinupristin/dalfopristin against intracellular pathogens and mycoplasmas. | quinupristin/dalfopristin displays in-vitro bacteriostatic activity against all legionella spp. (mics = 0.06-2 mg/l), with legionella pneumophila usually being at least two-fold more sensitive to quinupristin/dalfopristin than legionella bozemanii, legionella dumoffii, legionella gormanii and legionella micdadei (mic = 0.06-2 vs 1-2 mg/l, respectively). against legionella spp., quinupristin/dalfopristin was at least as active as erythromycin. quinupristin/dalfopristin was active in vitro against ... | 1997 | 9511064 |
| strand compositional asymmetry in bacterial and large viral genomes. | several bacterial genomes exhibit preference for g over c on the dna leading strand extending from the origin of replication to the ter-region in the genomes of escherichia coli, mycoplasma genitalium, bacillus subtilis, and marginally in haemophilus influenzae, mycoplasma pneumoniae, and helicobacter pylori. strand compositional asymmetry is not observed in the cyanobacterium synechocystis sp. genome nor in the archaeal genomes of methanococcus jannaschii, methanobacterium thermoautotrophicum, ... | 1998 | 9520433 |
| microbial genome analyses: global comparisons of transport capabilities based on phylogenies, bioenergetics and substrate specificities. | we have conducted genome sequence analyses of seven prokaryotic microorganisms for which completely sequenced genomes are available (escherichia coli, haemophilus influenzae, helicobacter pylori, bacillus subtilis, mycoplasma genitalium, synechocystis pcc6803 and methanococcus jannaschii). we report the distribution of encoded known and putative polytopic cytoplasmic membrane transport proteins within these genomes. transport systems for each organism were classified according to (1) putative me ... | 1998 | 9533881 |
| recognition of protein substrates by the prolyl isomerase trigger factor is independent of proline residues. | the trigger factor is associated with bacterial ribosomes and catalyzes proline-limited protein folding reactions. its folding activity is very high and conserved in evolution, as shown for the homologous enzymes from escherichia coli and mycoplasma genitalium. the folding protein substrate (a variant of ribonuclease t1) binds with high affinity to the trigger factors, and permanently unfolded proteins are strong, competitive inhibitors. we used this inhibition to characterize the substrate bind ... | 1998 | 9533890 |
| the frequency distribution of gene family sizes in complete genomes. | we compare the frequency distribution of gene family sizes in the complete genomes of six bacteria (escherichia coli, haemophilus influenzae, helicobacter pylori, mycoplasma genitalium, mycoplasma pneumoniae, and synechocystis sp. pcc6803), two archaea (methanococcus jannaschii and methanobacterium thermoautotrophicum), one eukaryote (saccharomyces cerevisiae), the vaccinia virus, and the bacteriophage t4. the sizes of the gene families versus their frequencies show power-law distributions that ... | 1998 | 9580988 |
| [detecting ureaplasma urealyticum, mycoplasma hominis and mycoplasma genitalium in the urogenital tract]. | to epidemiological study of ureaplasma urealyticum (uu), mycoplasma hominis (mh) and mycoplasma genitalium (mg) in the urogenital tract of different groups of people. | 1997 | 9596936 |
| protein identification with n and c-terminal sequence tags in proteome projects. | genome sequences are available for increasing numbers of organisms. the proteomes (protein complement expressed by the genome) of many such organisms are being studied with two-dimensional (2d) gel electrophoresis. here we have investigated the application of short n-terminal and c-terminal sequence tags to the identification of proteins separated on 2d gels. the theoretical n and c termini of 15, 519 proteins, representing all swiss-prot entries for the organisms mycoplasma genitalium, bacillus ... | 1998 | 9600841 |
| characterization of mgc2, a mycoplasma gallisepticum cytadhesin with homology to the mycoplasma pneumoniae 30-kilodalton protein p30 and mycoplasma genitalium p32. | a second cytadhesin-like protein, mgc2, was identified in the avian respiratory pathogen mycoplasma gallisepticum. the 912-nucleotide mgc2 gene encodes a 32.6-kda protein with 40.9 and 31.4% identity with the m. pneumoniae p30 and m. genitalium p32 cytadhesins, respectively. functional studies with reverse transcription-pcr, immunoblotting, double-sided immunogold labeling, and attachment inhibition assays demonstrated homology to the human mycoplasmal p30 and p32 cytadhesins. these findings sug ... | 1998 | 9632619 |
| the ftsz gene as a tool for detection of mycoplasma fermentans. | mycoplasma fermentans was reported as a common contaminant of cell cultures, and was shown to either induce or suppress several immunological functions. a strain of m. fermentans was recently isolated from a mouse t-lymphoma cell line, which differs from other m. fermentans strains by its growth characteristics and was designated (in the authors' records) as strain 609. using the differential display technique (dd), a differentially expressed gene that was identified as the m. fermentans 609 fts ... | 1998 | 9633043 |
| detection of mycoplasma genitalium in patients with urethritis. | we attempted to detect mycoplasma genitalium in urethral swab specimens by a polymerase chain reaction based assay to determine the prevalence of m. genitalium in patients with urethritis. | 1998 | 9649249 |
| homology-based fold predictions for mycoplasma genitalium proteins. | homology search techniques based on the iterative psi-blast method in combination with various filters for low sequence complexity are applied to assign folds to all mycoplasma genitalium proteins. the resulting procedure (implemented as a web server) is able to predict at least one domain in 37% of these proteins automatically, with an estimated accuracy higher than 98%. taking structural features such as coiled coil or transmembrane regions aside, folds can be assigned to more than half of the ... | 1998 | 9665839 |
| novel phosphotransferase system genes revealed by bacterial genome analysis: the complete complement of pts genes in mycoplasma genitalium. | the complete sequence of the mycoplasma genitalium chromosome has recently been determined. we here report analyses of the genes encoding proteins of the phosphoenolpyruvate:sugar phosphotransferase system, pts. these genes encode (1) enzyme i, (2) hpr, (3) a glucose-specific enzyme iicba, (4) an inactive glucose-specific enzyme iib, lacking the active site cysteyl residue, and (5) a fructose-specific enzyme iiabc. some of the unique features of these genes and their enzyme products are as follo ... | 1996 | 9689210 |
| fast comparison of a dna sequence with a protein sequence database. | we describe a computer program, named dna-protein search (dps), for comparing a megabase dna sequence with a protein sequence database. the dps program addresses the problems of frameshifts and introns in the dna sequence. the dps program was used to compare each of the following sequences with the swiss-prot database: the 1.8-megabase sequence of the haemophilus influenzae rd genome, the 0.58-megabase sequence of the mycoplasma genitalium genome, and the 0.56-megabase sequence of saccharomyces ... | 1996 | 9689213 |
| codon-anticodon assignment and detection of codon usage trends in seven microbial genomes. | we have assigned codon-anticodon recognition patterns for the whole set of transfer rnas of haemophilus influenzae rd, methanococcus jannaschii, and synechocystis sp. pcc6803 using sequence information derived from the complete genome sequence of these organisms and have tabulated them along with those previously reported for escherichia coli, mycoplasma genitalium, mycoplasma pneumoniae, and saccharomyces cerevisiae. using the resulting codon-anticodon tables, the bias in codon usage of genes e ... | 1997 | 9689228 |
| comparison of polymerase chain reaction assay with culture for detection of genital mycoplasmas in perinatal infections. | the polymerase chain reaction (pcr) technique was compared with culture for the detection of ureaplasma urealyticum, mycoplasma hominis, and mycoplasma genitalium in clinical samples (vaginal secretions, throat and endotracheal secretions, and skin swabs) obtained from 47 high-risk pregnant women peripartum and eight newborn infants. detection using pcr with homologous primers was highly specific, as a product with the expected length was consistently amplified with homologous but not with heter ... | 1998 | 9707308 |
| fold and function predictions for mycoplasma genitalium proteins. | uncharacterized proteins from newly sequenced genomes provide perfect targets for fold and function prediction. | 1998 | 9710568 |
| the subunit b of the f0f1-type atpase of the bacterium mycoplasma pneumoniae is a lipoprotein. | the dna sequence analysis of the f0f1-atpase operon of the bacterium mycoplasma pneumoniae predicted that the subunit b, encoded by the gene atpf, is a lipoprotein of the murein lipoprotein type of escherichia coli. here we experimentally verify this prediction by metabolic labeling of subunit b with [14c]palmitic acid and by in vivo interfering with the processing of the prolipoprotein form of subunit b by the antibiotic globomycin, a specific inhibitor of the signal peptidase ii. our results s ... | 1998 | 9733782 |
| evolution of the structure and chromosomal distribution of histidine biosynthetic genes. | a database of more than 100 histidine biosynthetic genes from different organisms belonging to the three primary domains has been analyzed, including those found in the now completely sequenced genomes of haemophilus influenzae, mycoplasma genitalium, synechocystis sp., methanococcus jannaschii, and saccharomyces cerevisiae. the ubiquity of his genes suggests that it is a highly conserved pathway that was probably already present in the last common ancestor of all extant life. the chromosomal di ... | 1998 | 9742729 |
| a genome-based approach for the identification of essential bacterial genes. | we have used comparative genomics to identify 26 escherichia coli open reading frames that are both of unknown function (hypothetical open reading frames or y-genes) and conserved in the compact genome of mycoplasma genitalium. not surprisingly, these genes are broadly conserved in the bacterial world. we used a markerless knockout strategy to screen for essential e. coli genes. to verify this phenotype, we constructed conditional mutants in genes for which no null mutants could be obtained. in ... | 1998 | 9743119 |
| assignment of folds for proteins of unknown function in three microbial genomes. | analysis of dna sequences of several microbial genomes has revealed that a large fraction of predicted coding regions has no known protein function. information about the three-dimensional folds of these proteins may provide insight into their possible functions. to predict the folds for protein sequences with little or no homology to proteins of known function, we used computational neural networks trained on the database of proteins with known three-dimensional structures. global descriptions ... | 1998 | 9775387 |
| genital mycoplasmas in patients with acquired immunodeficiency syndrome. | to determine whether mycoplasma fermentans. mycoplasma genitalium and mycoplasma penetrans were present in the genitourinary tract of hiv infected patients in nancy, france, we have used culture and polymerase chain reactions on urine from 54 hiv-infected patients. both techniques failed to reveal these bacteria. this renders their presence very unlikely in our population. | 1998 | 9812323 |
| analysis of complete genomes suggests that many prokaryotes do not rely on hairpin formation in transcription termination. | free energy values of mrna tertiary structures around stop codons were systematically calculated to surmise the hairpin-forming potential for all genes in each of the 16 complete prokaryote genomes. instead of trying to detect each individual hairpin, we averaged the free energy values around the stop codons over the entire genome to predict how extensively the organism relies on hairpin formation in the process of transcription termination. the free energy values of escherichia coli k-12 shows ... | 1998 | 9826772 |
| herpes simplex type ii and mycoplasma genitalium as risk factors for heterosexual hiv transmission: report from the heterosexual hiv transmission study. | two hundred twenty-four human immunodeficiency virus (hiv) discordant couples (one hiv negative, one hiv positive) were compared with 78 seroconcordant heterosexually infected couples with hiv with regard to sexually transmitted diseases. | 1998 | 9831669 |
| molecular biology and pathogenicity of mycoplasmas. | the recent sequencing of the entire genomes of mycoplasma genitalium and m. pneumoniae has attracted considerable attention to the molecular biology of mycoplasmas, the smallest self-replicating organisms. it appears that we are now much closer to the goal of defining, in molecular terms, the entire machinery of a self-replicating cell. comparative genomics based on comparison of the genomic makeup of mycoplasmal genomes with those of other bacteria, has opened new ways of looking at the evoluti ... | 1998 | 9841667 |
| structural assignments to the mycoplasma genitalium proteins show extensive gene duplications and domain rearrangements. | the parasitic bacterium mycoplasma genitalium has a small, reduced genome with close to a basic set of genes. as a first step toward determining the families of protein domains that form the products of these genes, we have used the multiple sequence programs psi-blast and geanfammer to match the sequences of the 467 gene products of m. genitalium to the sequences of the domains that form proteins of known structure [protein data bank (pdb) sequences]. pdb sequences (274) match all of 106 m. gen ... | 1998 | 9843945 |
| molecular and biochemical analysis of a 105 kda mycoplasma gallisepticum cytadhesin (gapa). | the identification of a gene (gapa) from mycoplasma gallisepticum with homology to the p1 cytadherence gene of mycoplasma pneumoniae is reported. the gapa gene is a 2895 bp orf encoding a protein with a molecular mass of 105 kda. nucleotide sequence analysis of the gapa gene revealed 45% homology to the m. pneumoniae p1 gene, 46% homology to the mycoplasma genitalium mgpa gene and 47% homology to the mycoplasma pirum p1-like protein gene. it has a 64 mol % a+t content compared to 46, 60 and 72 m ... | 1998 | 9846732 |
| the serine, threonine, and/or tyrosine-specific protein kinases and protein phosphatases of prokaryotic organisms: a family portrait. | inspection of the genomes for the bacteria bacillus subtilis 168, borrelia burgdorferi b31, escherichia coli k-12, haemophilus influenzae kw20, helicobacter pylori 26695, mycoplasma genitalium g-37, and synechocystis sp pcc 6803 and for the archaeons archaeoglobus fulgidus vc-16 dsm4304, methanobacterium thermoautotrophicum delta h, and methanococcus jannaschii dsm2661 revealed that each contains at least one orf whose predicted product displays sequence features characteristic of eukaryote-like ... | 1998 | 9862122 |
| genes from nine genomes are separated into their organisms in the dinucleotide composition space. | a set of 16 kinds of dinucleotide compositions was used to analyze the protein-encoding nucleotide sequences in nine complete genomes: escherichia coli, haemophilus influenzae, helicobacter pylori, mycoplasma genitalium, mycoplasma pneumoniae, synechocystis sp., methanococcus jannaschii, archaeoglobus fulgidus, and saccharomyces cerevisiae. the dinucleotide composition was significantly different between the organisms. the distribution of genes from an organism was clustered around its center in ... | 1998 | 9872449 |
| etiology of nongonococcal nonchlamydial urethritis. | the majority of cases of acute nongonococcal urethritis (ngu) are due to causes other than infection with chlamydia trachomatis. pathogens implicated as causes of nonchlamydial nongonococcal urethritis (ncngu) include mycoplasma genitalium, ureaplasma urealyticum, trichomonas vaginalis, and primary infection with herpes simplex virus. in a majority of cases of acute ncngu, no pathogen can be isolated. the etiology of chronic ncngu is unknown. | 1998 | 9891672 |
| systematic fold recognition analysis of the sequences encoded by the genome of mycoplasma pneumoniae. | a robust tool for fold recognition was applied to the systematic analysis of the sequences below 200 residues encoded by the genome of mycoplasma pneumoniae. the goal was to determine the additional information gain achievable in genome analysis by fold recognition, beyond the intrinsic limits of homology studies. a list of 124 sequences encoding for soluble proteins or domains not homologous to each other, or to proteins with known three-dimensional structure, was analyzed, resulting in signifi ... | 1998 | 9930662 |
| mycoplasma pneumoniae and mycoplasma genitalium: a comparison of two closely related bacterial species. | the rapid progress in sequencing large quantities of dna will provide an increasing number of complete genome sequences of closely related bacterial species as well as of pairs of isolates from the same species with different features, such as a pathogenic and an apathogenic representative. this opens the way to apply subtractive comparative analysis as a tool to select from the large pool of all bacterial genes a relatively small set of genes that can be correlated with the expression of a cert ... | 1998 | 10066529 |
| e-cell: software environment for whole-cell simulation. | genome sequencing projects and further systematic functional analyses of complete gene sets are producing an unprecedented mass of molecular information for a wide range of model organisms. this provides us with a detailed account of the cell with which we may begin to build models for simulating intracellular molecular processes to predict the dynamic behavior of living cells. previous work in biochemical and genetic simulation has isolated well-characterized pathways for detailed analysis, but ... | 1999 | 10068694 |
| identification of a ribonuclease h gene in both mycoplasma genitalium and mycoplasma pneumoniae by a new method for exhaustive identification of orfs in the complete genome sequences. | exhaustive identification of open reading frames in complete genome sequences is a difficult task. it is possible that important genes are missed. in our efforts to reanalyze the intergenic regions of mycoplasma genitalium and mycoplasma pneumoniae, we have newly identified a number of new open reading frames (orfs) in both m. genitalium and m. pneumoniae. the most significant identification was that of a ribonuclease h enzyme in both species which until now has not been identified or assumed ab ... | 1999 | 10069363 |
| functional insights from structural predictions: analysis of the escherichia coli genome. | fold assignments for proteins from the escherichia coli genome are carried out using basic, a profile-profile alignment algorithm, recently tested on fold recognition benchmarks and on the mycoplasma genitalium genome and psi blast, the newest generation of the de facto standard in homology search algorithms. the fold assignments are followed by automated modeling and the resulting three-dimensional models are analyzed for possible function prediction. close to 30% of the proteins encoded in the ... | 1999 | 10091664 |
| comparative study of overlapping genes in the genomes of mycoplasma genitalium and mycoplasma pneumoniae. | overlapping genes are defined, in this paper, as a pair of adjacent genes whose coding regions are partly overlapping. we systematically analyzed all overlapping genes in the genomes of two closely related species: mycoplasma genitalium and mycoplasma pneumoniae. careful comparisons were made for homologous genes that are overlapped in one species but not in the other. this comparative analysis allows us to propose a model of how overlapping genes emerged in the course of evolution. it was found ... | 1999 | 10101192 |
| genthreader: an efficient and reliable protein fold recognition method for genomic sequences. | a new protein fold recognition method is described which is both fast and reliable. the method uses a traditional sequence alignment algorithm to generate alignments which are then evaluated by a method derived from threading techniques. as a final step, each threaded model is evaluated by a neural network in order to produce a single measure of confidence in the proposed prediction. the speed of the method, along with its sensitivity and very low false-positive rate makes it ideal for automatic ... | 1999 | 10191147 |
| mycoplasma penetrans and other mycoplasmas in urine of human immunodeficiency virus-positive children. | urine samples from children with human immunodeficiency virus (hiv) infection and healthy controls were examined for mycoplasmas by culture. standard biochemical assays, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and pcr (16s and 16s-23s spacer rrna region) were used for identification of isolates. mycoplasmas were identified from 13 (87%) of 15 hiv-positive patients and 3 (20%) of 15 hiv-negative control patients. the frequency and type of mycoplasma varied with the severity of ... | 1999 | 10203515 |
| disruption of gene mg218 of mycoplasma genitalium through homologous recombination leads to an adherence-deficient phenotype. | although the complete genome of mycoplasma genitalium has been sequenced, the functional identification of various genes, including those involved in virulence, has not been accomplished. further compounding these difficulties has been the failure to develop genetic tools in mycoplasmas that permit the assessment of gene and operon function and regulation. to determine whether homologous recombination could be developed as a tool to analyze the function of genes in m. genitalium, a plasmid that ... | 1999 | 10220448 |
| [electrophoretic and immunologic comparative analysis of mycoplasma pneumoniae and mycoplasma genitalium proteins]. | the mycoplasma pneumoniae fh strain routinely used in our laboratory for over 25 years as antigen in serological tests, 2 reference m. pneumoniae strains from atcc (29342 and m129) and 3 isolates of m. pneumoniae obtained in 1995 from pneumonia patients were compared by sds-page, complement fixation test (cft) and by western-immunoblotting against human and rabbit serum samples with high level of mycoplasmal antibodies. on sds-page all m. pneumoniae strains showed the same number of 23 polypepti ... | 1998 | 10222741 |