| factors associated with asymptomatic non-chlamydial non-gonococcal urethritis in heterosexual men: findings from a case-control study. | the significance of asymptomatic non-chlamydial non-gonococcal urethritis (ncngu) is unclear. organisms associated with ncngu, e.g. mycoplasma genitalium, for which there is no widely available test, are linked to reproductive sequelae in women but uk guidance no longer recommends urethral smear microscopy to screen for asymptomatic ncngu. this case-control study of heterosexual male genitourinary (gu) medicine clinic attenders aimed to identify clinical, demographic and sexual behaviour factors ... | 2013 | 23970572 |
| mycoplasma genitalium and cancer: a brief review. | approximately, 15-20% of all cancers worldwide are caused by infectious agents. understanding the role of infectious agents on cancer development might be useful for developing new approaches to its prevention. mycoplasma genitalium is a clinically important sexually transmitted pathogen that has been associated with several human diseases. there have been a few studies suggestive of probable roles of mycoplasma genitalium in cancer development, including prostate and ovarian cancers and lymphom ... | 2013 | 23886122 |
| prevalence of mycoplasma genitalium among hiv-infected women, agence nationale de recherches sur le sida et les hépatites virales co3 aquitaine cohort, france. | | 2013 | 23859915 |
| mycoplasma genitalium and preterm delivery at an urban community health center. | to determine the prepartum prevalence of cervical mycoplasma genitalium colonization and evaluate prospectively whether colonization is associated with preterm delivery among women from a racial/ethnic minority background with a high risk of delivering a low birth weight newborn and a high prevalence of sexually transmitted infections. | 2013 | 23834822 |
| the immune effects of multiple antigen peptides containing the mimic epitopes of the adhesion protein of mycoplasma genitalium. | the purpose of this study was to investigate the humoral and cellular immune responses stimulated by multiple antigen peptides (maps) containing the mimic epitopes of mycoplasma genitalium adhesion protein (mgpa). three maps containing the mimic epitopes of mgpa were synthesized on a branched polylysine matrix. after purification and characterization, these maps were used to immunize balb/c mice. the immunoglobulin g (igg) antibody and the subtype of igg antibody in the serum of the immunized mi ... | 2013 | 23826957 |
| towards a whole-cell modeling approach for synthetic biology. | despite rapid advances over the last decade, synthetic biology lacks the predictive tools needed to enable rational design. unlike established engineering disciplines, the engineering of synthetic gene circuits still relies heavily on experimental trial-and-error, a time-consuming and inefficient process that slows down the biological design cycle. this reliance on experimental tuning is because current modeling approaches are unable to make reliable predictions about the in vivo behavior of syn ... | 2013 | 23822510 |
| treatment of mycoplasma genitalium. observations from a swedish std clinic. | to evaluate therapy for mycoplasma genitalium infection with doxycycline or azithromycin 1 g compared to five days of azithromycin (total dose 1.5 g). | 2013 | 23593483 |
| mycoplasma genitalium infection in sub-saharan africa: how big is the problem? | | 2013 | 23584806 |
| mycoplasma genitalium infection activates cellular host defense and inflammation pathways in a 3-dimensional human endocervical epithelial cell model. | because mycoplasma genitalium is a prevalent and emerging cause of sexually transmitted infections, understanding the mechanisms by which m. genitalium elicits mucosal inflammation is an essential component to managing lower and upper reproductive tract disease syndromes in women. | 2013 | 23493725 |
| contribution of topoisomerase iv mutation to quinolone resistance in mycoplasma genitalium. | the mechanism of quinolone resistance in mycoplasma genitalium remains poorly understood due to difficulties with in vitro culture, especially of clinical isolates. in this study, to confirm the association between mutations in topoisomerases and antimicrobial susceptibilities to quinolones, ciprofloxacin-resistant mutant strains were selected using the cultivable type strain atcc 33530. sequence analysis revealed that the mutant strains harbored mutations in topoisomerase iv: gly81cys in parc, ... | 2013 | 23357772 |
| comprehensive methylome characterization of mycoplasma genitalium and mycoplasma pneumoniae at single-base resolution. | in the bacterial world, methylation is most commonly associated with restriction-modification systems that provide a defense mechanism against invading foreign genomes. in addition, it is known that methylation plays functionally important roles, including timing of dna replication, chromosome partitioning, dna repair, and regulation of gene expression. however, full dna methylome analyses are scarce due to a lack of a simple methodology for rapid and sensitive detection of common epigenetic mar ... | 2013 | 23300489 |
| mycoplasma genitalium incidence, organism load, and treatment failure in a cohort of young australian women. | mycoplasma genitalium (mg) is an emerging sexually transmitted infection (sti) that is potentially associated with reproductive tract sequelae in women. this study aimed to estimate mg incidence and treatment failure and provide estimates of organism load in infection. | 2013 | 23300236 |
| wholecellkb: model organism databases for comprehensive whole-cell models. | whole-cell models promise to greatly facilitate the analysis of complex biological behaviors. whole-cell model development requires comprehensive model organism databases. wholecellkb (http://wholecellkb.stanford.edu) is an open-source web-based software program for constructing model organism databases. wholecellkb provides an extensive and fully customizable data model that fully describes individual species including the structure and function of each gene, protein, reaction and pathway. we u ... | 2013 | 23175606 |
| identification of treatment strategies for mycoplasma genitalium-related urethritis in male patients by culturing and antimicrobial susceptibility testing. | mycoplasma genitalium was first isolated from urethral swab specimens of male patients with non-gonococcal urethritis. however, the isolation of m. genitalium strains from clinical specimens has been difficult. co-cultivation with vero cells is one available technique for the isolation of m. genitalium. the strains that can be used for antimicrobial susceptibility testing by broth dilution or agar dilution methods are limited. macrolides, such as azithromycin (azm), have the strongest activity a ... | 2013 | 23076335 |
| risk behaviors and sti prevalence among people with hiv in el salvador. | to date, there are no studies from el salvador among people with hiv to inform prevention programs. we conducted a study in el salvador in 2008 among people with hiv using audio computer-assisted interviews on risk behaviors and access to health care. blood was tested for syphilis and herpes simplex type 2 (hsv-2). active syphilis was defined as rpr titer ≥1:8. genital specimens were tested for other sexually transmitted infections (sti) by pcr. we evaluated factors associated with unprotected s ... | 2012 | 23049671 |
| draft genome sequences of four axenic mycoplasma genitalium strains isolated from denmark, japan, and australia. | the dna genome of mycoplasma genitalium currently represents the smallest of all known human bacterial pathogens. despite their clinical importance in sexually transmitted infection and relevance as model bacterial pathogens, genomic diversity among m. genitalium strains worldwide is unknown. herein we present the complete draft genome sequences of four geographically diverse strains of m. genitalium. | 2012 | 23045512 |
| comparative bacterial genomics: defining the minimal core genome. | a comparative genomics analysis revealed 702 genes present in the bacterial gram-negative core gene set (92 species analyzed) and 959 genes in the gram-positive core gene set (93 species analyzed). mycoplasma genitalium, which has the smallest known genome (517 genes) of a non-symbiont, was used in a three-way reciprocal analysis with the gram-negative core genes and the gram-positive core genes, and 151 common bacterial core genes were found. of these 151 core genes, 39 were putative genes enco ... | 2013 | 23011009 |
| mycoplasma genitalium, an emerging sexually transmitted pathogen. | mycoplasma genitalium is a sexually transmitted organism associated with non-gonococcal urethritis in men and several inflammatory reproductive tract syndromes in women such as cervicitis, pelvic inflammatory disease, and infertility. there was evidence for an association of m. genitalium with endometritis and pelvic inflammatory disease (pid), but additional studies are necessary to confirm this. the evidence as to whether m. genitalium can cause adverse pregnancy outcomes such as preterm labor ... | 2012 | 22975074 |
| antimicrobial chemotherapy of mycoplasma genitalium-positive non-gonococcal urethritis. | mycoplasma genitalium is an important pathogen of acute non-gonococcal urethritis (ngu) in men and plays a significant role in persistent or recurrent ngu. in the management of patients with m. genitalium-positive ngu, eradication of the mycoplasma from the urethra is necessary to prevent persistent or recurrent ngu. therefore, m. genitalium should be considered for antimicrobial chemotherapy of ngu. this article reviews the in vitro antimicrobial activities of antibiotics against m. genitalium ... | 2012 | 22943402 |
| the eagr box structure: a motif involved in mycoplasma motility. | mycoplasma genitalium is an emerging human pathogen with the smallest genome found among self-replicating organisms. m. genitalium presents a complex cytoskeleton with a differentiated protrusion known as the terminal organelle. this polar structure plays a central role in functions essential for the virulence of the microorganism, such as motility and cell-host adhesion. a well-conserved enriched in aromatic and glycine residues motif, the eagr box, is present in many of the proteins found in t ... | 2012 | 22925012 |
| a whole-cell computational model predicts phenotype from genotype. | understanding how complex phenotypes arise from individual molecules and their interactions is a primary challenge in biology that computational approaches are poised to tackle. we report a whole-cell computational model of the life cycle of the human pathogen mycoplasma genitalium that includes all of its molecular components and their interactions. an integrative approach to modeling that combines diverse mathematics enabled the simultaneous inclusion of fundamentally different cellular proces ... | 2012 | 22817898 |
| the spread of mycoplasma genitalium among men who have sex with men. | we report two instances of urethral-to-rectal transmission of mycoplasma genitalium (mg) in men who have sex with men (msm) couples. such clear epidemiological correlation has to our knowledge not been published before. the urethral infections led to clinical symptoms, but the rectal infections did not. the rectum might serve as a reservoir for mg in msm, but there is also some evidence from the literature that mg can cause proctitis. our finding raises important questions about the role of mg a ... | 2012 | 22807547 |
| screening and identification of the mimic epitope of the adhesion protein of mycoplasma genitalium. | mycoplasma genitalium adhesion protein (mgpa) is the major adhesion protein of m. genitalium, and its c-terminal domain (amino acid 1075-1444) is the most immunogenic region. however, the exact epitopes of the adhesion protein of m. genitalium are still unclear. we used the purified polyclonal antibody against the recombinant adhesion protein to screen the mimic epitopes of mgpa using a random 12-peptide phage display library. immunoscreening via the phage display peptide library revealed that 3 ... | 2012 | 22716192 |
| the ruva homologues from mycoplasma genitalium and mycoplasma pneumoniae exhibit unique functional characteristics. | the dna recombination and repair machineries of mycoplasma genitalium and mycoplasma pneumoniae differ considerably from those of gram-positive and gram-negative bacteria. most notably, m. pneumoniae is unable to express a functional recu holliday junction (hj) resolvase. in addition, the ruvb homologues from both m. pneumoniae and m. genitalium only exhibit dna helicase activity but not hj branch migration activity in vitro. to identify a putative role of the ruva homologues of these mycoplasma ... | 2012 | 22666500 |
| discussion: 'mycoplasma genitalium in cervicitis and pelvic inflammatory disease' by bjartling et al. | | 2012 | 22631868 |
| novel one-step mechanism for trna 3'-end maturation by the exoribonuclease rnase r of mycoplasma genitalium. | mycoplasma genitalium is expected to metabolize rna using unique pathways because its minimal genome encodes very few ribonucleases. in this work, we report that the only exoribonuclease identified in m. genitalium, rnase r, is able to remove trna 3'-trailers and generate mature 3'-ends. several sequence and structural features of a trna precursor determine its precise processing at the 3'-end by rnase r in a purified system. the aminoacyl-acceptor stem plays a major role in stopping rnase r dig ... | 2012 | 22605341 |
| clinical characteristics associated with mycoplasma genitalium infection among women at high risk of hiv and other sti in uganda. | mycoplasma genitalium is a common infection of the genitourinary tract, but its pathogenic effects have not been well described, especially in women. the increasing evidence that m. genitalium is associated with hiv infection calls for an urgent consensus on how best to control this infection. the aim of this study was to describe symptoms and signs associated with m. genitalium infection among high-risk women in uganda. | 2012 | 22592838 |
| a comprehensive proteome of mycoplasma genitalium. | mycoplasma genitalium is a human pathogen associated with several sexually transmitted diseases. proteomic technologies, along with other methods for global gene expression analysis, play a key role in understanding the mechanisms of bacterial pathogenesis and physiology. the proteome of m. genitalium, model of a minimal cell, has been extended using a combination of different proteomic approaches and technologies. the total proteome of this microorganism has been analyzed using gel-based and ge ... | 2012 | 22582988 |
| methionine sulfoxide reductase a (msra) deficient mycoplasma genitalium shows decreased interactions with host cells. | mycoplasma genitalium is an important sexually transmitted pathogen that affects both men and women. in genital-mucosal tissues, it initiates colonization of epithelial cells by attaching itself to host cells via several identified bacterial ligands and host cell surface receptors. we have previously shown that a mutant form of m. genitalium lacking methionine sulfoxide reductase a (msra), an antioxidant enzyme which converts oxidized methionine (met(o)) into methionine (met), shows decreased vi ... | 2012 | 22558404 |
| transmission and selection of macrolide resistant mycoplasma genitalium infections detected by rapid high resolution melt analysis. | mycoplasma genitalium (mg) causes urethritis, cervicitis and pelvic inflammatory disease. the mg treatment failure rate using 1 g azithromycin at an australian sexual health clinic in 2007-9 was 31% (95%ci 23-40%). we developed a rapid high resolution melt analysis (hrma) assay targeting resistance mutations in the mg 23s rrna gene, and validated it against dna sequencing by examining pre- and post-treatment archived samples from mg-infected patients. | 2012 | 22532861 |
| recent perspectives in the diagnosis and evidence-based treatment of mycoplasma genitalium. | mycoplasma genitalium is a globally important sexually transmitted pathogen. men infected with m. genitalium frequently present with dysuria, while women may present with or without urogenital symptoms. in some populations, m. genitalium is significantly associated with hiv-1 infection, and is also an etiological agent in pelvic inflammatory disease. however, there is insufficient evidence to establish a causative role of the organism in obstetric complications, including tubal factor infertilit ... | 2012 | 22512757 |
| high prevalence of mycoplasma genitalium among female sex workers in honduras: implications for the spread of hiv and other sexually transmitted infections. | this study describes hiv, sexually transmitted infections (sti) and risk factors associated with mycoplasma genitalium among female sex workers (fsws) in four cities in honduras. in 2006, 795 fsws from tegucigalpa, san pedro sula, la ceiba and comayagua were recruited using respondent-driven sampling (rds) and tested for hiv prevalence and sti. hiv prevalence ranged from no infections in comayagua to 5.4% in tegucigalpa. with the exception of comayagua, more than 20% of fsws were infected with m ... | 2012 | 22362680 |
| the effect of premarital sex on sexually transmitted infections (stis) and high risk behaviors in women. | this research aimed to study the effect of premarital sex on sexually transmitted infections (stis) and high risk behaviors among women in sub-saharan africa. it included 1393 women randomly selected from the moshi urban district of northern tanzania. participants' demographic and socio-demographic characteristics, alcohol use, condom use, number of partners, symptoms of stis and age at first sex and marriage were obtained. moreover, blood and urine samples were tested for hiv-1, hsv-2, syphilis ... | 2013 | 23626920 |
| urinary meatal swabbing detects more men infected with mycoplasma genitalium and four other sexually transmitted infections than first catch urine. | urinary meatal swabs compared with urine showed higher infection rates for mycoplasma genitalium (15.3% vs 12.6%, p = 0.035), chlamydia trachomatis (11.3% vs 9.3%, p = 0.039), neisseria gonorrhoeae (1.4% vs 1.1%, p = 1.00), trichomonas vaginalis (8.0% vs 1.7%, p < 0.001), and high-risk human papillomavirus (5.9% vs 3.4%, p = 0.078) respectively. | 2017 | 28703728 |
| mycoplasma genitalium in women: current knowledge and research priorities for this recently emerged pathogen. | health consequences of sexually transmitted diseases disproportionately affect women, making it important to determine whether newly emerged pathogens cause sequelae. although the pathogenic role of mycoplasma genitalium in male urethritis is clear, fewer studies have been conducted among women to determine its pathogenic role in the female reproductive tract. pelvic inflammatory disease (pid) is an important cause of infertility and ectopic pregnancy, and chlamydia trachomatis and neisseria gon ... | 2017 | 28838078 |
| the unique microbiology and molecular pathogenesis of mycoplasma genitalium. | mycoplasma genitalium is increasingly appreciated as a common cause of sexually transmitted disease syndromes, including urethritis in men and cervicitis, endometritis, pelvic inflammatory disease, and possibly preterm birth, tubal factor infertility, and ectopic pregnancy in women. despite these disease associations, which parallel those of chlamydia trachomatis and neisseria gonorrhoeae, the mechanisms by which this pathogen elicits inflammation, causes cellular damage, and persists in its onl ... | 2017 | 28838077 |
| mycoplasma genitalium infection in men. | mycoplasmagenitalium is one of the major causes of nongonococcal urethritis (ngu) worldwide but an uncommon sexually transmitted infection (sti) in the general population. the risk of sexual transmission is probably lower than for chlamydia trachomatis. infection in men is usually asymptomatic and it is likely that most men resolve infection without developing disease. the incubation period for ngu caused by mycoplasma genitalium is probably longer than for ngu caused by c. trachomatis. the clin ... | 2017 | 28838074 |
| the detection of microorganisms related to urethritis from the oral cavity of male patients with urethritis. | to investigate the presence of microorganisms related to urethritis in the oral cavity of male patients with urethritis and the efficacies of antimicrobials for urethritis on microorganisms in the oral cavity. | 2017 | 28803864 |
| assessing the need for routine screening for mycoplasma genitalium in the low-risk female population: a prevalence and co-infection study on women from croatia. | there is an ongoing debate regarding possible cost and benefits, but also harm of universal screening for the emerging sexually transmitted pathogen mycoplasma genitalium. | 2017 | 28757928 |
| prevalence and antibiotic resistance of mycoplasma genitalium among sti clinic attendees in western canada: a cross-sectional analysis. | to determine the prevalence and correlates of mycoplasma genitalium (mg) infection among men and women, determine the prevalence of gene mutations conferring resistance and compare test performance of female specimen types. | 2017 | 28698342 |
| uriswab: an effective transport medium for nucleic acid detection of chlamydia trachomatis, mycoplasma genitalium and neisseria gonorrhoeae. | background: patient self-sampling allows for remote collection and return to clinic or laboratory by post. urine samples, although convenient, are challenging to post. this study evaluated uriswab (copan, brescia, italy) as a collection and transport vessel for chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and mycoplasma genitalium (mg) detection by polymerase chain reaction, compared with flocked swab and neat urine. methods: five replicates of each specimen type were prepared from pre ... | 2017 | 28697843 |
| prevalence of chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium and ureaplasma urealyticum infections using a novel isothermal simultaneous rna amplification testing method in infertile males. | the purpose of this study was to evaluate the prevalence of chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium and ureaplasma urealyticum infections in infertile men that consulted our outpatient departments using a novel simultaneous amplification testing (sat) that is rna-detection based. the possible impact of c. trachomatis, n. gonorrhoeae, m. genitalium and u. urealyticum infections on semen parameters was also noted in the present study. | 2017 | 28646898 |
| mycoplasma genitalium antibiotic resistance-mediating mutations in canadian women with or without chlamydia trachomatis infection. | testing remnant aptima specimens from women infected with chlamydia trachomatis detected 13.4% (53/396) with mycoplasma genitalium compared with 5.4% (22/406) in matched c. trachomatis-negative women. overall, 9.4% (provincial ranges of 3-20%) were infected with m. genitalium and resistance mediating mutations were found in 47.3% (26/55) to macrolides and 1.9% (1/53) to fluoroquinolones by sequencing. | 2017 | 28608794 |
| evaluation of self-collected rectal swabs for the detection of bacteria responsible for sexually transmitted infections in a cohort of hiv-1-infected patients. | the standard approach to screening sexually transmitted infections (stis) has often been restricted to urogenital specimens. most current guidelines, however, also recommend testing extra-genital sites, including rectal locations, because asymptomatic rectal carriage of pathogens has often been reported. the aim of our study was to evaluate self-collected rectal swabs to screen bacterial stis in hiv-infected patients in marseille, france. | 2017 | 28590237 |
| mycoplasma genitalium presence, resistance and epidemiology in greenland. | objectives . greenland reports the highest rates of chlamydial infection and gonorrhea in the arctic. our objective was to determine the presence, and describe the basic epidemiology, of mycoplasma genitalium for greenland. study design . cross-sectional study. methods . 314 residents from nuuk and sisimiut, between the ages of 15 and 65 years, participated in "inuulluataarneq" (the greenland sexual health project) between july 2008 and november 2009. participants provided self-collected samples ... | 2012 | 28417753 |
| women with symptoms of a urinary tract infection but a negative urine culture: pcr-based quantification of escherichia coli suggests infection in most cases. | our objective was to examine whether or not women with symptoms of a urinary tract infection but with a negative culture (20%-30%) do have an infection. | 2017 | 28457846 |
| mycoplasma hominis and mycoplasma genitalium in the vaginal microbiota and persistent high-risk human papillomavirus infection. | recent studies have suggested that the vaginal microenvironment plays a role in persistence of high-risk human papillomavirus (hrhpv) infection and thus cervical carcinogenesis. furthermore, it has been shown that some mycoplasmas are efficient methylators and may facilitate carcinogenesis through methylation of hrhpv and cervical somatic cells. we examined associations between prevalence and persistence of mycoplasma spp. in the vaginal microbiota, and prevalent as well as persistent hrhpv infe ... | 2017 | 28695118 |
| the potential role of infectious agents and pelvic inflammatory disease in ovarian carcinogenesis. | the etiological cause of ovarian cancer is poorly understood. it has been theorized that bacterial or viral infection as well as pelvic inflammatory disease could play a role in ovarian carcinogenesis. | 2017 | 28529540 |
| developing a public health response to mycoplasma genitalium. | although mycoplasma genitalium is increasingly recognized as a sexually transmitted pathogen, at present there is no defined public health response to this relatively newly identified sexually transmitted infection. currently available data are insufficient to justify routinely screening any defined population for m. genitalium infection. more effective therapies, data on acceptability of screening and its impact on clinical outcomes, and better information on the natural history of infection wi ... | 2017 | 28838079 |
| mycoplasma genitalium from basic science to public health implications: results of a national institute of allergy and infectious diseases technical consultation. | | 2017 | 28838076 |
| mycoplasma genitalium from basic science to public health: summary of the results from a national institute of allergy and infectious disesases technical consultation and consensus recommendations for future research priorities. | this article lays out the research priorities for mycoplasma genitalium research agreed upon by the participants in a 2016 national institutes of allergy and infectious diseases-funded technical consultation focused on this organism. the state of current knowledge concerning the microbiology, epidemiology, clinical manifestations of infection, treatment, and public health significance of m. genitalium reviewed at the meeting is described in detail in the individual articles included in this supp ... | 2017 | 28838075 |
| new horizons in mycoplasma genitalium treatment. | mycoplasmagenitalium is an important sexually transmitted pathogen responsible for both male and female genital tract disease. appreciation of its significance in human disease has been hampered by its slow growth in culture, difficulty in isolating it, and lack of commercial molecular-based tests for rapid detection. comparatively few in vitro data on antimicrobial susceptibility are available due to the scarcity of clinical isolates and difficulty in performing susceptibility tests to determin ... | 2017 | 28838073 |
| mycoplasma genitalium: accurate diagnosis is necessary for adequate treatment. | mycoplasma genitalium is very difficult to grow in culture but has been more able to be studied for disease associations since the advent of research molecular amplification assays. polymerase chain reaction (pcr) and other molecular assays have demonstrated an association with adverse disease outcomes, such as urethritis or nongonococcal urethritis in men and adverse reproductive sequelae in women-for example, cervicitis, endometritis, and pelvic inflammatory disease (pid), including an associa ... | 2017 | 28838072 |
| french prospective clinical evaluation of the aptima® mycoplasma genitalium assay (ce-ivd) and macrolide resistance detection using three distinct assays. | the aim of this study was to evaluate the clinical performance of the aptima® mycoplasma genitalium ce-ivd (mg-tma) assay for the detection of mycoplasma genitalium in clinical male and female samples in comparison with the in-house real-time pcr (in-house pcr) assay routinely used in our laboratory. a total of 1,431 clinical specimens obtained from 1,235 patients were prospectively collected at the bacteriology department of bordeaux university hospital (france). additional research-use-only ap ... | 2017 | 28794183 |
| in vitro activities of gepotidacin (gsk2140944) and other antimicrobial agents against human mycoplasmas and ureaplasmas. | gepotidacin, a novel, first in class, triazaacenaphthylene topoisomerase ii inhibitor, was tested against 85 type strains and clinical isolates of mycoplasma pneumoniae, mycoplasma hominis, mycoplasma genitalium, ureaplasma parvum, and ureaplasma urealyticum in comparison to levofloxacin, moxifloxacin, azithromycin or clindamycin, and tetracycline. gepotidacin mic90s (μg/ml) were: m. pneumoniae (0.125), m. genitalium (0.032), m. hominis (2), and ureaplasma species (8). gepotidacin activity was n ... | 2017 | 28784668 |
| mycoplasma genitalium, an agent of reemerging sexually transmitted infections. | m. genitalium is a reemerging microorganism, responsible for sexually transmissible infections (stis), with prevalence which varies depending on the country and population group studied. we report here m. genitalium prevalence among the specimens received for sti diagnosis in our routine microbiological laboratory in the university hospital in marseille, france. we tested 4 624 samples from 3 793 patients using qpcr for m. genitalium, c. trachomatis, n. gonorrheae, t. pallidum. of these samples, ... | 2017 | 28762558 |
| molecular diagnostics update for the emerging (if not already widespread) sexually-transmitted infection agent mycoplasma genitalium: just about ready for prime time. | mycoplasma genitalium is an important and emerging agent of sexually-transmitted infection in both females and males, carrying the potential for post-infection genital tract sequelae. past efforts to identify this organism on a routine basis, which were problematic due to the fastidious nature of the bacterium and its antigenic intricacies, have recently become supplemented by molecular diagnostics. a number of these assays is available commercially. this mini-review describes the format and per ... | 2017 | 28724558 |
| detection of markers predictive of macrolide and fluoroquinolone resistance in mycoplasma genitalium from patients attending sexual health services in england. | resistance to both macrolides and fluoroquinolones has been reported in mycoplasma genitalium; however, due to limited diagnostics, studies are often small and confined to specific geographical areas. this study sought to determine the rate of predicted resistance in m. genitalium-positive specimens referred for diagnostic testing. | 2017 | 28717051 |
| which azithromycin regimen should be used for treating mycoplasma genitalium? a meta-analysis. | there is increasing evidence that azithromycin 1 g is driving the emergence of macrolide resistance in mycoplasma genitalium worldwide. we undertook a meta-analysis of m. genitalium treatment studies using azithromycin 1 g single dose and azithromycin 500 mg on day 1 then 250 mg daily for 4 days (5-day regimen) to determine rates of treatment failure and resistance in both regimens. | 2017 | 28717050 |
| prevalence of cervical colonization by ureaplasma parvum, ureaplasma urealyticum, mycoplasma hominis and mycoplasma genitalium in childbearing age women by a commercially available multiplex real-time pcr: an italian observational multicentre study. | mycoplasmas are frequently isolated from the genital tract. new molecular pcr-based methods for the detection of mycoplasmas can better define the real epidemiology of these microorganisms. the aim of this study was to evaluate the prevalence of mycoplasmas in a population of childbearing age women by means of pcr. | 2017 | 28711440 |
| mycoplasma genitalium on the loose: time to sound the alarm. | | 2017 | 28703724 |
| mycoplasma genitalium macrolide and fluoroquinolone resistance: prevalence and risk factors among a 2013-2014 cohort of patients in barcelona, spain. | macrolide and fluoroquinolone resistance is alarmingly emerging in m. genitalium worldwide. this article provides the first estimates of the current prevalence of macrolide and fluoroquinolone resistance-mediating mutations in barcelona, spain, and identifies risk factors associated with the acquisition of these resistances. | 2017 | 28703723 |
| mycoplasma genitalium: yet another challenging sti. | | 2017 | 28701270 |
| rapid accurate point-of-care tests combining diagnostics and antimicrobial resistance prediction for neisseria gonorrhoeae and mycoplasma genitalium. | in addition to inadequate access to early diagnosis and treatment with antimicrobial agents for patients and sexual contacts, management and control of stis is significantly challenged by emergence and spread of antimicrobial resistance (amr), particularly for stis such as neisseria gonorrhoeae and mycoplasma genitalium this is further compounded by use of nucleic acid amplification techniques for diagnosis, resulting in reduced phenotypic amr testing for n. gonorrhoeae and absence or suboptimal ... | 2017 | 28684610 |
| mycoplasma genitalium and macrolide resistance-associated mutations in the skåne region of southern sweden 2015. | mycoplasma genitalium is a sexually transmitted infection ordinarily treated with azithromycin. emerging resistance to macrolide is linked to mutations in the 23s rrna gene. we analysed the frequency of such mutations of m. genitalium isolates from patients that were symptomatic, and from sexual partners of symptomatic individuals, from october to december of 2015, in the skåne region of sweden. mutations were analysed by the use of dna sequencing. overall, 11.9% (145/1311) and 17.0% (116/704) o ... | 2017 | 28681067 |
| structural characterization of the nap; the major adhesion complex of the human pathogen mycoplasma genitalium. | mycoplasma genitalium, the causative agent of non-gonococcal urethritis and pelvic inflammatory disease in humans, is a small eubacterium that lacks a peptidoglycan cell wall. on the surface of its plasma membrane is the major surface adhesion complex, known as nap that is essential for adhesion and gliding motility of the organism. here, we have performed cryo-electron tomography of intact cells and detergent permeabilized m. genitalium cell aggregates, providing sub-tomogram averages of free a ... | 2017 | 28671286 |
| cost-effectiveness of microscopy of urethral smears for asymptomatic mycoplasma genitalium urethritis in men in england. | the objective was to determine whether or not the limited use of urethral microscopy to diagnose asymptomatic and symptomatic non-chlamydial, non-gonococcal urethritis (ncngu) in men is a cost-effective strategy to avert pelvic inflammatory disease (pid), ectopic pregnancy or infertility in female partners. outputs from a transmission dynamic model of ncngu in a population of 16-30 year olds in england simulating the number of consultations, pid cases and patients treated over time amongst other ... | 2017 | 28669322 |
| mycoplasma genitalium. | | 2017 | 28640054 |
| the immunopathogenesis of mycoplasma genitalium infections in women: a narrative review. | mycoplasma genitalium is a common, predominately asymptomatic, and often undiagnosed sexually transmitted infection that is associated with inflammatory urogenital and reproductive tract disease syndromes of men and women. without programmatic screening in the united states, and with increasing resistance to antibiotics used in empiric sexually transmitted infection management, undiagnosed m. genitalium infections put many women at risk for cervicitis and pelvic inflammatory disease. chronic inf ... | 2017 | 28608793 |
| association of recent bacterial vaginosis with acquisition of mycoplasma genitalium. | we assessed the association between recent bacterial vaginosis (bv) and incident mycoplasma genitalium, a sexually transmitted bacterium associated with adverse female reproductive health outcomes. female sex workers in mombasa, kenya, completed a monthly sexual behavior interview and clinical examination. during february 2005-february 2006, vaginal fluid specimens collected from women every other month were tested for m. genitalium by nucleic acid amplification testing. vaginal microbiota were ... | 2017 | 28472225 |
| high macrolide resistance in mycoplasma genitalium strains causing infection in auckland, new zealand. | | 2017 | 28468853 |
| emergence of mycoplasma genitalium with clinically significant fluoroquinolone resistance conferred by amino acid changes both in gyra and parc in japan. | we observed fluoroquinolone treatment failures in 2 men with mycoplasma genitalium-positive non-gonococcal urethritis in japan. a fluoroquinolone regimen of sitafloxacin 100 mg twice daily for 7 days failed to eradicate m. genitalium. in both cases, m. genitalium had fluoroquinolone resistance-associated amino acid changes both in gyra and parc and a macrolide resistance-associated mutation in the 23s rrna gene. the emergence of such multi-drug resistant strains can threaten antimicrobial chemot ... | 2017 | 28462860 |
| isolation of mycoplasma genitalium from endocervical swabs of infertile women. | to determine the occurrence of mycoplasma genitalium (m. genitalium) infection in infertile women attending infertility clinic and its association to infertility. methods: endocervical specimens were collected from women presenting with primary and secondary infertility and from fertile women as control group. real-time polymerase chain reaction (pcr) assay was performed to detect the presence of m. genitalium from these endocervical specimens. results: mycoplasma genitalium was detected in 3% ... | 2017 | 28439608 |
| macrolide and quinolone-resistant mycoplasma genitalium in a man with persistent urethritis: the tip of the british iceberg? | there is growing concern worldwide for macrolide resistance in m. genitalium following liberal use of 1 g azithromycin to treat non-gonococcal urethritis and confirmed c. trachomatis infection. moxifloxacin is the second-line treatment for m. genitalium and still has excellent efficacy against it. however, recent reports indicating that quinolone resistance is more prevalent than previously thought are worrying. routine testing of symptomatic men and women for m. genitalium is not currently reco ... | 2017 | 28438948 |
| increasing macrolide and fluoroquinolone resistance in mycoplasma genitalium. | escalating resistance to azithromycin and moxifloxacin is being reported for mycoplasma genitalium in the asia-pacific region. analyzing 140 infections, we found pretreatment fluoroquinolone-resistance mutations in parc (13.6%) and gyra (5%). parc s83 changes were associated with moxifloxacin failure. combined macrolide/fluoroquinolone-resistance mutations were in 8.6% of specimens, for which recommended therapies would be ineffective. | 2017 | 28418319 |
| prevalence of macrolide and fluoroquinolone resistance-mediating mutations in mycoplasma genitalium in five cities in russia and estonia. | resistance in the sexually transmitted bacterium mycoplasma genitalium to all recommended therapeutic antimicrobials have rapidly emerged. however, to date, internationally reported resistance surveillance data for m. genitalium strains circulating in eastern europe are entirely lacking. the aim of this study was to estimate the prevalence of macrolide and fluoroquinolone resistance-associated mutations in m. genitalium in four cities in russia and one in estonia, 2013-2016. | 2017 | 28407014 |