| internet-based recruitment system for hiv and sti screening for men who have sex with men in estonia, 2013: analysis of preliminary outcomes. | the aim of the current project was to develop an internet-based recruitment system for hiv and sexually transmitted infection (sti) screening for men who have sex with men (msm) in estonia in order to collect biological samples during behavioural studies. in 2013, an internet-based hiv risk-behaviour survey was conducted among msm living in estonia. after completing the questionnaire, all participants were offered anonymous and free-of-charge sti testing. they could either order a urine sample k ... | 2015 | 25953131 |
| sexual partnership characteristics of african american women who have sex with women; impact on sexually transmitted infection risk. | african american women who have sex with women (wsw) are emerging as a population at risk for sexually transmitted infections (stis). the objectives of this study were to explore partnership characteristics for a cohort of african american wsw and evaluate those characteristics as potential risk factors for stis. in addition, we aimed to determine sti diagnoses and identify predictors of sti infection. | 2014 | 25211257 |
| detection of macrolide resistance in mycoplasma genitalium in france. | mycoplasma genitalium is a sexually transmitted organism associated with non-gonococcal urethritis in men and several inflammatory reproductive tract syndromes in women. resistance to macrolides has been recently associated with point mutations in the 23s rrna gene. the aim of this study was to detect these mutations using a large french collection of m. genitalium-positive specimens. we evaluated whether these mutations were related to azithromycin treatment failure and whether macrolide-resist ... | 2012 | 22773740 |
| mycoplasma genitalium is associated with increased genital hiv type 1 rna in zimbabwean women. | mycoplasma genitalium is a common sexually transmitted infection associated with human immunodeficiency virus (hiv) infection. some studies suggest that m. genitalium may increase the risk of hiv acquisition. however, results have been inconsistent, and this association has never been examined longitudinally. | 2015 | 25404521 |
| the common vaginal commensal bacterium ureaplasma parvum is associated with chorioamnionitis in extreme preterm labor. | to assess the association of vaginal commensal and low-grade pathogenic bacteria including ureaplasma parvum, ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium, group b streptococcus (gbs), and gardnerella vaginalis, in women who delivered preterm at less than 37-week gestation in the presence or absence of inflammation of the chorioamnionitic membranes. | 2016 | 26795404 |
| anorectal lymphogranuloma venereum in madrid: a persistent emerging problem in men who have sex with men. | since 2003, outbreaks of lymphogranuloma venereum (lgv) with anorectal syndrome have been increasingly recognized in many western countries. all of them have been classified as lgv serovar l2b, mainly occurring in human immunodeficiency virus (hiv)-infected men who have had sex with men (msm). we describe a series of 26 diagnosed cases of lgv proctitis in downtown madrid, spain, in 2014, after implementing routine diagnostic procedures for this disease in symptomatic msm. | 2016 | 27322040 |
| alarming incidence of genital mycoplasmas among hiv-1-infected msm in jiangsu, china. | males who have sex with men (msm) are considered at high risk of blood-borne and sexually transmitted infections (stis), mainly due to the practice of unsafe sex, often combined with drug use and needle-sharing. a cross-sectional study was designed for the detection of genital mycoplasmas during the period from march 2009 to may 2010 in jiangsu province. this work was approved by the research ethics committee of jiangsu centers for diseases prevention and control (cdc), and written consent was o ... | 2014 | 23949791 |
| histological evidence of chronic mycoplasma genitalium-induced cervicitis in hiv-infected women: a retrospective cohort study. | mycoplasma genitalium is an emerging sexually transmitted pathogen implicated in inflammatory syndromes of the female reproductive tract. the objective of this study was to investigate human immunodeficiency virus (hiv)-infected women for an association between m. genitalium and cervicitis, a putative mechanism for enhanced hiv transmission efficiency to an uninfected partner. | 2016 | 26783349 |
| the contribution of mycoplasma genitalium to the aetiology of sexually acquired infectious proctitis in men who have sex with men. | this study examined the contribution of mycoplasma genitalium to sexually acquired infectious proctitis in men who have sex with men (msm). msm with symptomatic proctitis between may 2012 and august 2013 were tested for rectal sexually transmitted infections including chlamydia, gonorrhoea, herpes simplex virus (hsv) and m. genitalium. the load of rectal m. genitalium in men with symptomatic proctitis was compared with a separate group of men who had rectal m. genitalium but no symptoms of proct ... | 2016 | 26686807 |
| mycoplasma genitalium promotes epithelial crossing and peripheral blood mononuclear cell infection by hiv-1. | mycoplasma genitalium co-infection in hiv-infected individuals has been reported to increase the shedding of hiv in the urogenital region of females. to better understand this relationship, we investigated the influence of m. genitalium on the transmission and replication of hiv using an in vitro model. | 2014 | 24661929 |
| high-risk human papillomavirus messenger rna testing in physician- and self-collected specimens for cervical lesion detection in high-risk women, kenya. | little is known about the performance of physician-versus self-collected specimens for high-risk human papillomavirus (hrhpv) messenger rna (mrna) testing or risk factors for hrhpv mrna positivity in physician- versus self-collected specimens. we compared the performance of hrhpv mrna testing of physician- and self-collected specimens for detecting cytological high-grade squamous intraepithelial lesions or more severe (≥hsil) and examined risk factors for hrhpv mrna positivity in female sex work ... | 2013 | 23965776 |
| mycoplasma genitalium in spain: prevalence of genital infection and frequency of resistance to macrolides. | the aim of this study was to determine the prevalence of mycoplasma genitalium infection and the resistance to macrolides within a general population in madrid in 2015. | 2017 | 28291669 |
| microbial invasion of the amniotic cavity in midtrimester pregnancies using molecular microbiology. | the objective of the study was to determine the frequency of microbial invasion of the amniotic cavity in the midtrimester of pregnancy in patients undergoing amniocentesis for clinical indications. | 2017 | 28268197 |
| mollicutes in vaginal microbiology: mycoplasma hominis, ureaplasma urealyticum, ureaplasma parvum and mycoplasma genitalium. | mycoplasma hominis was isolated in 1937 from the human genital tract, followed 17 years later by ureaplasma urealyticum and 27 years after that by mycoplasma genitalium. the first two proved relatively easy to culture but the latter required a polymerase chain reaction assay for further studies. in sexually mature women, m. hominis may be found in the vagina/cervix of about 20-50%, ureaplasmas in 40-80% and m. genitalium in 0-5%. some heterogeneity has been found among strains of all these speci ... | 2017 | 28263902 |
| microscopy of stained urethral smear in male urethritis; which cutoff should be used? | the microscopical diagnosis of male urethritis was recently questioned by rietmeijer and mettenbrink, lowering the diagnostic criteria of the diagnosis to ≥2 polymorphonuclear leucocytes (pmnl) per high power field (hpf), and adopted by centers for disease control and prevention in their 2015 std treatment guidelines. the european non-gonococcal urethritis guideline advocates a limit of ≥5 pmnl/hpf. | 2017 | 28178118 |
| high prevalence of the a2058t macrolide resistance-associated mutation in mycoplasma genitalium strains from the netherlands. | | 2017 | 28158595 |
| meta-analysis of the efficacy of moxifloxacin in treating mycoplasma genitalium infection. | mycoplasma genitalium is an important pathogen that is transmitted through sexual contact. for patients diagnosed with m. genitalium infection, the current guidelines recommend 1 g of azithromycin as the first-line treatment. moxifloxacin is used as a second-line drug due to its remarkable efficacy; however, increased use of moxifloxacin to treat m. genitalium infections has caused the emergence of cases of moxifloxacin treatment failure. this meta-analysis aims to estimate the treatment efficac ... | 2017 | 28118803 |
| superior multiplexing capacity of plexprimers enables sensitive and specific detection of snps and clustered mutations in qpcr. | whilst qpcr provides an extremely powerful tool for genetic analysis, some applications such as multiplexing variant alleles (eg snps, point mutations or deletions), remain challenging using current primer/probe systems. the novel design features of plexprimers allow sensitive, multiplexed analysis of variant alleles even when these are tightly clustered. | 2017 | 28114309 |
| mycoplasma/ureaplasma infection in pregnancy: to screen or not to screen. | mycoplasmata have been linked to pregnancy complications and neonatal risk. while formerly a limited number of species could be discovered by cultures, molecular biology nowadays discovers both lower quantities and more diverse species, making us realize that mycoplasmata are ubiquitous in the vaginal milieu and do not always pose a danger for pregnant women. as the meaning of mycoplasmata in pregnancy is not clear to many clinicians, we summarized the current knowledge about the meaning of diff ... | 2017 | 28099135 |
| antimicrobial-resistant sexually transmitted infections: gonorrhoea and mycoplasma genitalium. | the emergence of antimicrobial resistance (amr) is a major concern worldwide and already compromises treatment effectiveness and control of several bacterial sexually transmitted infections (stis). neisseria gonorrhoeae and mycoplasma genitalium are evolving into so-called superbugs that can become resistant, both in vitro and clinically, to essentially all antimicrobials available for treatment, causing exceedingly difficult-to-treat or untreatable stis and threatening global public health. wid ... | 2017 | 28072403 |
| evaluation of a commercial multiplex quantitative pcr (qpcr) assay for simultaneous detection of mycoplasma genitalium and macrolide resistance-associated mutations in clinical specimens. | | 2017 | 28031435 |
| azithromycin 1.5g over 5 days compared to 1g single dose in urethral mycoplasma genitalium: impact on treatment outcome and resistance. | we evaluated the impact of extended azithromycin (1.5g over 5 days) on selection of macrolide resistance and microbiological cure in men with mycoplasma genitalium urethritis during 2013-2015 and compared this to cases treated with azithromycin 1g in 2012-2013. | 2017 | 28011607 |
| clinical laboratory assessments for mycoplasma genitalium in a high-prevalence sexually-transmitted infection community reveal epidemiologic dichotomies with trichomonas vaginalis. | mycoplasma genitalium is an emerging agent of sexually-transmitted infection and is responsible for clinically-significant genital tract disease in both females and males. similar to scenarios recently experienced with the urogenital flagellate trichomonas vaginalis, an evolving molecular diagnostic reference standard based on transcription-mediated amplification allows for accurate detection of the organism, plus additional insight into disease epidemiology. areas covered. the basis for this ar ... | 2017 | 27894204 |
| prevalence of mycoplasma genitalium infection and relationship with symptoms among adults attending a sexual health centre. | is missing (short communication). | 2017 | 27882384 |
| experimental infection of pig-tailed macaques (macaca nemestrina) with mycoplasma genitalium. | mycoplasma genitalium is an underappreciated cause of human reproductive tract disease, characterized by persistent, often asymptomatic, infection. building on our previous experiments using a single female pig-tailed macaque as a model for m. genitalium infection (g. e. wood, s. l. iverson-cabral, d. l. patton, p. k. cummings, y. t. cosgrove sweeney, and p. a. totten, infect immun 81:2938-2951, 2013, https://doi.org/10.1128/iai.01322-12), we cervically inoculated eight additional animals, two o ... | 2017 | 27872239 |
| thermodynamics of macromolecular association in heterogeneous crowding environments: theoretical and simulation studies with a simplified model. | the cytoplasm of a cell is crowded with many different kinds of macromolecules. the macromolecular crowding affects the thermodynamics and kinetics of biological reactions in a living cell, such as protein folding, association, and diffusion. theoretical and simulation studies using simplified models focus on the essential features of the crowding effects and provide a basis for analyzing experimental data. in most of the previous studies on the crowding effects, a uniform crowder size is assume ... | 2016 | 27797534 |
| [mycoplasma genitalium]. | | 2016 | 27773499 |
| background review for the 2016 european guideline on mycoplasma genitalium infections. | mycoplasma genitalium is a cause of 10-35% of non-chlamydial non-gonococcal urethritis in men and in women, and is associated with cervicitis and pelvic inflammatory disease (pid). transmission of m. genitalium occurs through direct mucosal contact. in women, symptoms include vaginal discharge, dysuria or symptoms of pid - abdominal pain and dyspareunia. in men, urethritis, dysuria and discharge predominates. asymptomatic infections are frequent. in this review, we present the evidence base for ... | 2016 | 27605499 |
| 2016 european guideline on mycoplasma genitalium infections. | mycoplasma genitalium infection contributes to 10-35% of non-chlamydial non-gonococcal urethritis in men. in women, m. genitalium is associated with cervicitis and pelvic inflammatory disease (pid). transmission of m. genitalium occurs through direct mucosal contact. asymptomatic infections are frequent. in women, symptoms include vaginal discharge, dysuria or symptoms of pid - abdominal pain and dyspareunia. in men, urethritis, dysuria and discharge predominates. besides symptoms, indication fo ... | 2016 | 27505296 |
| successful outcome of macrolide-resistant mycoplasma genitalium urethritis after spectinomycin treatment: a case report. | | 2017 | 27707988 |
| antimicrobial susceptibility patterns of recent cuban mycoplasma genitalium isolates determined by a modified cell-culture-based method. | isolation of mycoplasma genitalium from clinical specimens remains difficult and few strains are available for antimicrobial susceptibility testing. we describe the antimicrobial susceptibility of m. genitalium strains grown in vero cell culture with first- and second- line antibiotics, using a modified cell-culture-based method. macrolide- and -fluoroquinolone resistance determinants were detected by sequencing of the 23s and parc genes, respectively. seven strains were examined, including thre ... | 2017 | 27679992 |
| sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in jalisco, mexico. | to determine the frequency of nine sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in jalisco, mexico. | 2016 | 27599076 |
| fluoroquinolone-resistant mycoplasma genitalium, southwestern france. | | 2016 | 27533360 |
| emergence of mycoplasma genitalium strains showing mutations associated with macrolide and fluoroquinolone resistance in the region dresden, germany. | among 323 specimens from male patients with symptoms of non-gonococcal urethritis, mycoplasma genitalium was detected in 19 samples by real-time pcr. mutations of 23s rrna gene associated with macrolide resistance were confirmed in 10 strains. amino acid changes at positions 81 and 83 of parc protein were demonstrated indicating quinolone resistance of two strains. | 2016 | 27448452 |
| guidelines for reproducibly building and simulating systems biology models. | reproducibility is the cornerstone of the scientific method. however, currently, many systems biology models cannot easily be reproduced. this paper presents methods that address this problem. | 2016 | 27429432 |
| prevalence and correlates of mycoplasma genitalium infection among prostatitis patients in shanghai, china. | background: mycoplasma genitalium (m. genitalium) has been shown to be involved in chronic non-gonococcal urethritis (ngu). however, the prevalence and determinants of this emerging sexually transmissible infection among prostatitis patients remain obscure. methods: two hundred and thirty-five patients diagnosed with prostatitis and 152 health controls from sexually transmitted diseases (std) clinics in shanghai, china, were selected. m. genitalium was detected in the initial voided urine (vb1), ... | 2016 | 27372963 |
| evaluation of the hologic panther transcription-mediated amplification assay for detection of mycoplasma genitalium. | the detection of mycoplasma genitalium was evaluated on 1,080 urine samples by the use of a panther instrument. overall sensitivity, specificity, positive predictive values, and negative predictive values were 100%, 99.4%, 93.6%, and 100%, respectively. detection of m. genitalium by the use of the panther transcription-mediated amplification assay offers a simple, accurate, and sensitive platform for diagnostic laboratories. | 2016 | 27307453 |
| prevalence of urogenital mycoplasmas among men with ngu in upper silesia, poland. preliminary study. | the prevalence of urogenital mycoplasmas in men with ngu in upper silesia (poland) was studied. mycoplasmas were detected in 36.7% men (ureaplasma parvum and mycoplasma genitalium were found in 30% and 16.7% respectively). urealyticum urealyticum was not detected. we suggest including m. genitalium in the diagnostic scheme for nongonococcal urethritis (ngu). | 2016 | 27281999 |
| mycoplasmataceae colonizations in women with urethral pain syndrome: a case-control study. | to determine if mycoplasma genitalium, ureaplasma urealyticum, and ureaplasma parvum are more common in premenopausal women with urethral pain syndrome than in asymptomatic controls. | 2016 | 27172116 |
| emerging sexually transmitted diseases: hepatitis c, lymphogranuloma venereum (lgv), and mycoplasma genitalium infections. | | 2016 | 27109045 |
| characteristics of pelvic inflammatory disease where no sexually transmitted infection is identified: a cross-sectional analysis of routinely collected sexual health clinic data. | pelvic inflammatory disease (pid) occurs when pathogens, often sexually transmitted, ascend to the upper genital tract, yet a causative pathogen is not detected in a substantial proportion of diagnosed pid. we assessed the characteristics associated with pid in women in whom chlamydia, gonorrhoea, mycoplasma genitalium (mg) and bacterial vaginosis (bv) were not detected ('pathogen-negative-pid'). | 2017 | 27091727 |
| all-in-one construct for genome engineering using cre-lox technology. | mycoplasma genitalium is an appealing model of a minimal cell and synthetic biology study, and it was one of the first organisms whose genome was fully sequenced and chemically synthesized. despite its usefulness as a model organism, many genetic tools well established for other microorganisms are not currently available in mycoplasmas. we have developed several vectors to adapt the cre-lox technology for genome engineering in m. genitalium, providing an all-in-one construct that could be also u ... | 2016 | 27084897 |
| structure-guided mutations in the terminal organelle protein mg491 cause major motility and morphologic alterations on mycoplasma genitalium. | the emergent human pathogen mycoplasma genitalium, with one of the smallest genomes among cells capable of growing in axenic cultures, presents a flask-shaped morphology due to a protrusion of the cell membrane, known as the terminal organelle, that is involved in cell adhesion and motility and is an important virulence factor of this microorganism. the terminal organelle is supported by a cytoskeleton complex of about 300 nm in length that includes three substructures: the terminal button, the ... | 2016 | 27082435 |
| the prevalence of six species of mycoplasmataceae in an hiv/aids population in jiangsu province, china. | this study employed culture and polymerase chain reaction (pcr) to examine the prevalence of ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium, mycoplasma fermentans, mycoplasma penetrans and mycoplasma pirum in 210 hiv/aids patients, 455 sexually transmitted infection (sti) clinic attendees and 245 healthy volunteers from first-void urine specimens for men and endocervical swabs for women. u. urealyticum and m. hominis were detected in 107 (51.0%) and 69 (32.9%) patients in the ... | 2012 | 22930310 |
| prospective evaluation of resistanceplus™ mg, a new multiplex qpcr assay for the detection of mycoplasma genitalium and macrolide resistance. | mycoplasma genitalium is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. as conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23s rrna gene have been described to predict resistance. recently a novel multiplex qpcr assay, resistanceplus™ mg, has been described for the simultaneous detection of mycop ... | 2017 | 28381611 |
| multidrug-resistant mycoplasma genitalium infections in europe. | in japan and australia, multidrug-resistant mycoplasma genitalium infections are reported with increasing frequency. although macrolide-resistant m. genitalium strains are common in europe and north america, fluoroquinolone-resistant strains are still exceptional. however, an increase of multidrug-resistant m. genitalium in europe and america is to be expected. the aim of this paper is to increase awareness on the rising number of multidrug-resistant m. genitalium strains. here, one of the first ... | 2017 | 28361246 |
| a 5' nuclease genotyping assay for identification of macrolide-resistant mycoplasma genitalium in clinical specimens. | rapid and sensitive detection of macrolide resistance in mycoplasma genitalium is required for the guidance of adequate antimicrobial treatment. previous studies have confirmed that single-base mutations at position 2058 or 2059 in domain v of the 23s rrna gene of m. genitalium result in high-level macrolide resistance. sequencing of pcr products remains the gold standard for the identification of mutations conferring resistance to macrolides but is laborious and time-consuming. the aim of the p ... | 2016 | 27053672 |
| apparently-different clearance rates from cohort studies of mycoplasma genitalium are consistent after accounting for incidence of infection, recurrent infection, and study design. | mycoplasma genitalium is a potentially major cause of urethritis, cervicitis, pelvic inflammatory disease, infertility, and increased hiv risk. a better understanding of its natural history is crucial to informing control policy. two extensive cohort studies (students in london, uk; ugandan sex workers) suggest very different clearance rates; we aimed to understand the reasons and obtain improved estimates by making maximal use of the data from the studies. as m. genitalium is a sexually-transmi ... | 2016 | 26910762 |
| mycoplasma genitalium: a possible case of macrolide resistance from lebanon. | | 2016 | 26873160 |
| screening for mycoplasma genitalium infection? | | 2016 | 26813809 |
| mycoplasma genitalium: the need for testing and emerging diagnostic options. | | 2015 | 26742263 |
| prevalence and antibiotic susceptibility of mycoplasma hominis and ureaplasma urealyticum in genital samples collected over 6 years at a serbian university hospital. | mycoplasma hominis and ureaplasma urealyticum are implicated in a wide array of infectious diseases in adults and children. since some species have innate or acquired resistance to certain types of antibiotics, antibiotic susceptibility testing of mycoplasma isolated from the urogenital tract assumes increasing importance. | 2016 | 26728808 |
| a minimized motile machinery for mycoplasma genitalium. | the cell wall-less bacterium mycoplasma genitalium uses specialized adhesins located at the terminal organelle to adhere to host cells and surfaces. the terminal organelle is a polar structure protruding from the cell body that is internally supported by a cytoskeleton and also has an important role in cell motility. we have engineered a m. genitalium null mutant for mg491 protein showing a massive downstream destabilization of proteins involved in the terminal organelle organization. this mutan ... | 2016 | 26712501 |
| kinetics of genetic variation of the mycoplasma genitalium mg192 gene in experimentally infected chimpanzees. | mycoplasma genitalium, a human pathogen associated with sexually transmitted diseases, is capable of causing chronic infections, though mechanisms for persistence remain unclear. previous studies have found that variation of the mgpa operon occurs by recombination of repetitive chromosomal sequences (known as mgpars) into the mg191 and mg192 genes carried on this operon, which may lead to antigenic variation and immune evasion. in this study, we determined the kinetics of mg192 sequence variatio ... | 2015 | 26712208 |
| efficacy of antimicrobial therapy for mycoplasma genitalium infections. | mycoplasma genitalium has been causally linked with nongonococcal urethritis in men and cervicitis, pelvic inflammatory disease, preterm birth, spontaneous abortion, and infertility in women, yet treatment has proven challenging. to inform treatment recommendations, we reviewed english-language studies describing antimicrobial susceptibility, resistance-associated mutations, and clinical efficacy of antibiotic therapy, identified via a systematic search of pubmed supplemented by expert referral. ... | 2015 | 26602619 |
| characteristics of adenovirus urethritis among heterosexual men and men who have sex with men: a review of clinical cases. | the aim of this study was to characterise the clinical features of adenovirus urethritis in men and to compare the frequency of these between heterosexual men and men who have sex with men (msm). | 2016 | 26574571 |
| multidrug-resistant mycoplasma genitalium is increasing. | | 2016 | 26494825 |
| mycoplasma and ureaplasma infection and male infertility: a systematic review and meta-analysis. | the relationship between mycoplasma and ureaplasma infection and male infertility has been studied widely; however, results remain controversial. this meta-analysis investigated the association between genital ureaplasmas (ureaplasma urealyticum, ureaplasma parvum) and mycoplasmas (mycoplasma hominis, mycoplasma genitalium), and risk of male infertility. differences in prevalence of ureaplasma and mycoplasma infection between china and the rest of the world were also compared. study data were co ... | 2015 | 26311339 |
| management of mycoplasma genitalium infections - can we hit a moving target? | mycoplasma genitalium is an etiological agent of sexually transmitted infections, but due to its fastidious growth requirements, only a few m. genitalium strains are available for determination of the activity of currently used and new antimicrobial agents.recent clinical trials have demonstrated that treatment with azithromycin has decreasing efficacy due to an increasing prevalence of macrolide resistance, which is likely to be attributed to the widespread use of 1 g single dose azithromycin. ... | 2015 | 26286546 |
| time to eradication of mycoplasma genitalium after antibiotic treatment in men and women. | the objectives of this study were to evaluate the time to a mycoplasma genitalium-negative test after start of treatment and to monitor if and when antibiotic resistance developed. | 2015 | 26283670 |
| a combined systems and structural modeling approach repositions antibiotics for mycoplasma genitalium. | bacteria are increasingly resistant to existing antibiotics, which target a narrow range of pathways. new methods are needed to identify targets, including repositioning targets among distantly related species. we developed a novel combination of systems and structural modeling and bioinformatics to reposition known antibiotics and targets to new species. we applied this approach to mycoplasma genitalium, a common cause of urethritis. first, we used quantitative metabolic modeling to identify en ... | 2015 | 26271684 |
| editorial commentary: mycoplasma genitalium and declining treatment efficacy of azithromycin 1 g: what can we do? | | 2015 | 26240204 |
| the efficacy of azithromycin for the treatment of genital mycoplasma genitalium: a systematic review and meta-analysis. | mycoplasma genitalium (mg) is associated with nongonococcal urethritis in men and cervicitis in women. current guidelines recommend treatment with 1 gram of azithromycin; however, treatment failure has increasingly been reported. this meta-analysis estimates treatment efficacy following treatment with 1 gram of azithromycin. | 2015 | 26240201 |
| loop-mediated isothermal amplification (lamp) for the rapid detection of mycoplasma genitalium. | mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. due to the difficulty of the culture of m. genitalium from clinical samples, the laboratory diagnosis of m. genitalium infection is almost exclusively carried out using nucleic acid amplification tests. loop-mediated isothermal amplification (lamp) is a novel nucleic acid amplification technology, utilising a set of 4 primers specific to 6 distinct re ... | 2015 | 26072150 |
| sexually transmitted diseases treatment guidelines, 2015. | these guidelines for the treatment of persons who have or are at risk for sexually transmitted diseases (stds) were updated by cdc after consultation with a group of professionals knowledgeable in the field of stds who met in atlanta on april 30-may 2, 2013. the information in this report updates the sexually transmitted diseases treatment guidelines, 2010 (mmwr recomm rep 2010;59 [no. rr-12]). these updated guidelines discuss 1) alternative treatment regimens for neisseria gonorrhoeae; 2) the u ... | 2015 | 26042815 |
| high levels of macrolide resistance-associated mutations in mycoplasma genitalium warrant antibiotic susceptibility-guided treatment. | mycoplasma genitalium is a sexually transmitted pathogen, and infection with it is usually treated with macrolides. unfortunately, emerging resistance to the macrolides has been associated with mutations in region v of the 23s rrna gene. the aim of this retrospective study was to describe the incidence of macrolide resistance-associated mutations in m. genitalium from patients in the netherlands. | 2015 | 25995292 |
| detection of genital mycoplasmal infections among infertile females by multiplex pcr. | women reproductive system is a suitable environment for growth of various pathogen and nonpathogenic microorganisms. mycoplasmataceae is a family of bacteria which cause oligosymptomatic genital infections. the complications caused by these bacteria may lead to infertility in women. the aim of this study was detection of genital mycoplasma hominis, ureaplasmaurealyticum and mycoplasma genitalium among infertile females who referred to the infertility clinics. | 2014 | 25926957 |
| a novel sigma factor reveals a unique regulon controlling cell-specific recombination in mycoplasma genitalium. | the mycoplasma genitalium mg428 protein shows homology to members of the sigma-70 family of sigma factors. herein, we found that mg428 activates transcription of reca, ruva and ruvb as well as several genes with unknown function. deletion of mg_428 or some of the up-regulated unknown genes led to severe recombination defects. single cell analyses revealed that activation of the mg428-regulon is a rare event under laboratory growth conditions. a conserved sequence with sigma-70 promoter architect ... | 2015 | 25925568 |
| mycoplasma genitalium infection and female reproductive tract disease: a meta-analysis. | to determine the association between mycoplasma genitalium infection and female reproductive tract syndromes through meta-analysis, english-language, peer-reviewed studies were identified via pubmed, embase, biosis, cochrane library, and reference review. two reviewers independently extracted data. random-effects models were employed to calculate summary estimates, between-study heterogeneity was evaluated using i(2) statistics, publication bias was assessed via funnel plots and the begg and egg ... | 2015 | 25900174 |
| development and clinical application of an invaderplus® assay for the detection of genital mycoplasmas. | we developed a pcr-based assay involving invader® technology for detection of the genital mycoplasmas of mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum, and ureaplasma parvum. we compared its performance with that of a pcr-microtiter plate hybridization assay, which we developed previously, in detecting genital mycoplasmas in first-voided urine (fvu) specimens from men with non-gonococcal urethritis. the tests targeting each of the genital mycoplasmas were specific for the res ... | 2015 | 25892209 |
| complete atomistic model of a bacterial cytoplasm for integrating physics, biochemistry, and systems biology. | a model for the cytoplasm of mycoplasma genitalium is presented that integrates data from a variety of sources into a physically and biochemically consistent model. based on gene annotations, core genes expected to be present in the cytoplasm were determined and a metabolic reaction network was reconstructed. the set of cytoplasmic genes and metabolites from the predicted reactions were assembled into a comprehensive atomistic model consisting of proteins with predicted structures, rna, protein/ ... | 2015 | 25765281 |
| prevalence and macrolide resistance of mycoplasma genitalium in south african women. | remnant specimens from 601 women obtained in a cross-sectional study from rural south africa were tested for mycoplasma genitalium. overall, 10.8% of women were infected with m. genitalium either in the vagina or in the rectum. macrolide resistance, although of low prevalence, in m. genitalium is described for the first time in sub-saharan africa. | 2015 | 25668646 |
| [mycoplasma genitalium-derived lipid-associated membrane proteins negatively regulate cytokine secretion by inducing ho-1 expression in placental trophoblast cells]. | to observe the expression of heme oxygenase-1 (ho-1) in regulation of cytokines response induced by mycoplasma genitalium-derived lipid-associated membrane proteins (lamps) in placental trophoblast cells. | 2015 | 25652861 |
| spontaneous regression of untreatable mycoplasma genitalium urethritis. | | 2015 | 25634824 |
| macrolide resistance and azithromycin failure in a mycoplasma genitalium-infected cohort and response of azithromycin failures to alternative antibiotic regimens. | our aim was to determine the efficacy of 1 g azithromycin and alternative antibiotic regimens in a prospective cohort of mycoplasma genitalium-infected participants, and factors associated with azithromycin failure. | 2015 | 25537875 |
| [mycoplasma genitalium lipid-associated membrane proteins induce endocervical epithelial cells to express β-defensin-2]. | to observe the expression of human β-defensin-2 (hbd-2) induced by mycoplasma genitalium-derived lipid-associated membrane proteins (lamps) and its potential mechanism. | 2014 | 25481183 |
| a major determinant for gliding motility in mycoplasma genitalium: the interaction between the terminal organelle proteins mg200 and mg491. | several mycoplasmas, such as the emergent human pathogen mycoplasma genitalium, developed a complex polar structure, known as the terminal organelle (to), responsible for a new type of cellular motility, which is involved in a variety of cell functions: cell division, adherence to host cells, and virulence. the to cytoskeleton is organized as a multisubunit dynamic motor, including three main ultrastructures: the terminal button, the electrodense core, and the wheel complex. here, we describe th ... | 2015 | 25471372 |
| isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female. | mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. the infections influenced form genital mycoplasmas specifically mycoplasma hominis and mycoplasma genitalium potentially affect reproductive disorders, and infertility. | 2014 | 25469132 |
| [non-gonococcal infectious urethritis : pathogen spectrum and management]. | for many years an increase in cases of urethritis has been observed in western europe. in order to be able to combat this continuous rise, the perception of sexually transmitted diseases must be promoted, the clarification and screening must be intensified and therapy must be rapidly and correctly carried out. in addition to the commonest pathogens causing urethritis, namely chlamydia and gonococci, many other pathogenic microbes must be taken into consideration in the diagnostics. with respect ... | 2015 | 25410827 |
| mycoplasma genitalium and its resistance to azithromycin in incarcerated men from far north queensland. | this study examined the prevalence of mycoplasma genitalium in incarcerated men from far north queensland as well as the prevalence of macrolide resistance in identified isolates. overall, eight out of 140 [5.71% (95% ci 1.82-9.60)] urine samples tested positive and two out of eight (25%) samples carried a mutation in the 23s rrna gene associated with macrolide resistance. | 2014 | 25402451 |
| development and utilization of a custom pcr array workflow: analysis of gene expression in mycoplasma genitalium and guinea pig (cavia porcellus). | transcriptome analysis is a powerful tool for evaluating molecular pathways central to maturation of specific biological processes and disease states. recently, pcr-based arrays have supplemented microarray and rna-seq methodologies for studying changes in gene expression levels. pcr arrays are a more cost efficient alternative, however commercially available assemblies are generally limited to only a few more widely researched species (e.g., rat, human, and mouse). consequently, the investigati ... | 2015 | 25358686 |
| sitafloxacin: antimicrobial activity against ciprofloxacin-selected laboratory mutants of mycoplasma genitalium and inhibitory activity against its dna gyrase and topoisomerase iv. | a sitafloxacin regimen is highly effective on mycoplasma genitalium infections, including those caused by the mycoplasmas harboring mutant topoisomerase iv with a quinolone resistance-associated amino acid change in parc. in this study, we evaluated sitafloxacin antimicrobial activities against m. genitalium, including the mycoplasmas with decreased susceptibilities to quinolones, by determining minimum inhibitory concentrations (mics) for the strain atcc 33530 and its 3 ciprofloxacin-selected m ... | 2015 | 25245990 |
| does cervical ureaplasma/mycoplasma colonization increase the lower uterine segment bleeding risk during cesarean section among patients with placenta previa? a cross-sectional study. | the underlying inflammation of endometrium may impede normal implantation of placenta during pregnancy. our objective is to show cervical colonization of ureaplasma and/or mycoplasma as a marker of endometritis in pregnancies complicated with placenta previa that can be a risk factor for placenta accreta and peripartum hemorrhage. | 2014 | 25219820 |
| positivity at test of cure following first-line treatment for genital mycoplasma genitalium: follow-up of a clinical cohort. | to describe antibiotic use for treatment of mycoplasma genitalium (mg) at an urban sexual health centre in australia. to describe mg positivity rates in those returning for 1 month test of cure (toc) following first-line antibiotic treatment for mg. | 2015 | 25096921 |
| remarkable increase in fluoroquinolone-resistant mycoplasma genitalium in japan. | we determined the prevalence of macrolide and fluoroquinolone resistance-associated mutations in mycoplasma genitalium dna specimens from men with non-gonococcal urethritis (ngu) and analysed their effects on antibiotic treatments of m. genitalium infections. | 2014 | 24894419 |
| uncoupling of the apyrimidinic/apurinic endonucleolytic and 3'→5' exonucleolytic activities of the nfo protein of mycoplasma pneumoniae through mutation of specific amino acid residues. | the dna recombination and repair machineries of mycoplasma pneumoniae and mycoplasma genitalium were predicted to consist of a set of ~11 proteins. the function of one of these proteins was inferred from its homology with proteins belonging to the endo iv enzyme family. the members of this family function in the repair of apyrimidinic/apurinic (ap) sites in dna. as such activity may be crucial in the mycoplasmal life cycle, we set out to study the endo iv-like proteins encoded by m. pneumoniae a ... | 2014 | 24694374 |
| epidemiological and clinical rationale for screening and diagnosis of mycoplasma genitalium infections. | | 2014 | 24669447 |
| the prevalence of urethral and rectal mycoplasma genitalium among men who have sex with men in china, a cross-sectional study. | although mycoplasma genitalium (mg) is a common sexually transmitted infection (sti), very little information regarding the prevalence of mg among msm (men who have sex with men) is available in china. the objective of this study was to determine the prevalence of mg among msm in the city of shenzhen, guangdong province, china, and to identify the potential risk factors associated with mg infection in this population. | 2014 | 24559387 |
| the future of whole-cell modeling. | integrated whole-cell modeling is poised to make a dramatic impact on molecular and systems biology, bioengineering, and medicine--once certain obstacles are overcome. from our group's experience building a whole-cell model of mycoplasma genitalium, we identified several significant challenges to building models of more complex cells. here we review and discuss these challenges in seven areas: first, experimental interrogation; second, data curation; third, model building and integration; fourth ... | 2014 | 24556244 |
| mycoplasma genitalium: a review. | mycoplasma genitalium (m. genitalium)was first isolated from the urethral swabs of two symptomatic men with urethritis in 1980. published prevalence rates vary greatly between populations studied. a number of urogenital conditions have been ascribed tom. genitalium, which is recognised to cause a sexually transmitted infection. the association of m. genitalium with non-specific urethritis is now well established, but the evidence supporting its role in both male and female infertility remains in ... | 2014 | 24517928 |
| prediction of the persistence of mycoplasma genitalium after antimicrobial chemotherapy by quantification of leukocytes in first-void urine from patients with non-gonococcal urethritis. | mycoplasma genitalium is regarded as another pathogen of male non-gonococcal urethritis (ngu). failure to eradicate this mycoplasma is associated with persistent or recurrent ngu, but this mycoplasma is not routinely examined in clinical practice. in cases of m. genitalium-positive ngu, therefore, some criteria are needed to assess the success or failure of antimicrobial chemotherapy other than microbiological outcomes. we enrolled 49 men with m. genitalium-positive non-chlamydial ngu. at succes ... | 2014 | 24508423 |
| mycoplasma genitalium: clinical significance and diagnosis. | mycoplasma genitalium is considered the smallest self-replicating cell. it was first isolated in 1981, from 2 of 13 men with urethritis. mycoplasma genitalium causes urethritis, cervicitis and pelvic inflammatory disease. because of difficulties in cultivation, the diagnosis is based exclusively on pcr methodology. the recommended therapy for mycoplasma genitalium infections is azithromycin or doxycycline. development of macrolide resistance was shown to correlate with treatment failure. | 2013 | 24476610 |
| an epidemiological study of mycoplasma genitalium infections among males attending a sexually transmitted disease clinic in guangxi, china. | the objective of this study was to determine the prevalence of urethral mycoplasma genitalium infections among male patients attending a sexually transmitted disease (std) clinic in china and identify risk factors associated with this disease. a total of 423 patients were recruited in hezhou city, guangxi province, china, and each was requested to complete a questionnaire regarding sociological and sexual behaviors. first-void urine samples were collected for m. genitalium analysis by polymerase ... | 2014 | 24451096 |
| time to manage mycoplasma genitalium as an sti: but not with azithromycin 1 g! | mycoplasma genitalium is a sexually transmitted infection that causes significant morbidity in men and women and is a co-factor in hiv transmission. however, commercial diagnostic tests are not generally available for m. genitalium and sub-optimal treatment is often given. we review the literature on the burden of infection, how it may present in clinical practice and the effectiveness of current treatment regimens. | 2014 | 24322592 |
| genome sequence of mycoplasma parvum (formerly eperythrozoon parvum), a diminutive hemoplasma of the pig. | we report the complete genome sequence of mycoplasma parvum strain indiana. its circular chromosome is 564,395 bp, which is smaller than that of mycoplasma genitalium, which was previously considered the smallest member of the mollicutes. comparative analyses of the genomes of m. parvum and mycoplasma suis will provide novel insights into the molecular basis of their virulence. | 2013 | 24285648 |
| mycoplasma genitalium: an emergent sexually transmitted disease? | this article summarizes the epidemiologic evidence linking mycoplasma genitalium to sexually transmitted disease syndromes, including male urethritis, and female cervicitis, pelvic inflammatory disease, infertility, and adverse birth outcomes. it discusses the relationship of this bacterium to human immunodeficiency virus infection and reviews the available literature on the efficacy of standard antimicrobial therapies against m genitalium. | 2013 | 24275270 |
| accelerated discovery via a whole-cell model. | to test the promise of whole-cell modeling to facilitate scientific inquiry, we compared growth rates simulated in a whole-cell model with experimental measurements for all viable single-gene disruption mycoplasma genitalium strains. discrepancies between simulations and experiments led to predictions about kinetic parameters of specific enzymes that we subsequently validated. these findings represent, to our knowledge, the first application of whole-cell modeling to accelerate biological discov ... | 2013 | 24185838 |
| binding of cd14 to mycoplasma genitalium-derived lipid-associated membrane proteins upregulates tnf-α. | lipid-associated membrane proteins (lamps) are a mixture of mycoplasmal lipoproteins expressed on the surface, and they are the main structures for interaction with the host cells. the objective of this study was to explore the role of cd14 in immune recognition of mycoplasma genitalium-derived lamps and investigate whether the binding of cd14 to lamps affects the inflammatory response. enzyme-linked immunosorbent assay (elisa), transient co-transfection, dual-luciferase reporter assay, specific ... | 2014 | 24068451 |
| failure of moxifloxacin treatment in mycoplasma genitalium infections due to macrolide and fluoroquinolone resistance. | increasing azithromycin treatment failure in sexually transmitted mycoplasma genitalium infection, is linked to macrolide resistance and second-line treatment relies on the fluoroquinolone, moxifloxacin. we recently detected fluoroquinolone and macrolide resistance-associated mutations in 15% and 43%, respectively, of 143 initial m. genitalium pcr-positive specimens. for a subset of 33 western sydney sexual health centre patients, clinical information and results of sequence analysis of m. genit ... | 2013 | 24052013 |
| reactive arthritis associated with mycoplasma genitalium urethritis. | mycoplasma genitalium is an important cause of sexually transmitted infections that is gaining recognition and is an independent cause of acute and chronic nongonococcal urethritis in men. m. genitalium has been implicated as a possible causative factor in reactive arthritis. we report a case of reactive arthritis complicating m. genitalium urethritis in an hla-b27-positive patient. | 2013 | 24034901 |