| Survey of marbofloxacin susceptibility of bacteria isolated from cattle with respiratory disease and mastitis in Europe. | A monitoring programme conducted in Europe since 1994 to survey the marbofloxacin susceptibility of bacterial pathogens isolated from cattle has established the susceptibility of bacterial strains isolated before any antibiotic treatment from bovine mastitis and bovine respiratory disease (BRD) cases between 2002 and 2008. Minimum inhibitory concentration (MIC) was determined by a standardised microdilution technique. For respiratory pathogens, Pasteurella multocida and Mannheimia haemolytica is ... | 2011 | 22121154 |
| serosurvey of bacterial and viral respiratory pathogens among deployed u.s. service members. | respiratory illnesses can cause substantial morbidity during military deployments. bordetella pertussis, chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, parainfluenza, and respiratory syncytial virus (rsv) are hypothesized causes. | 2011 | 22099233 |
| stevens-johnson syndrome associated with cytomegalovirus infection in a child with ependymoma. | stevens-johnson syndrome is an uncommon, acute life-threatening disease characterized by extensive epidermal sloughing and mucositis. in childhood, as in adulthood, this condition is mostly related to drugs, in particular antibiotics. only a few cases reported were firmly attributed to infectious agents, mainly mycoplasma pneumonia but the causative role of infectious microorganisms seems particularly relevant in pediatric patients. the seriousness of this condition imposes a prompt recognition ... | 2010 | 21886739 |
| gamma irradiation of animal sera for inactivation of viruses and mollicutes--a review. | animal-derived materials such as animal sera represent a low, but finite, risk for introduction of an adventitious agent (virus or mollicute) into a biological bulk harvest during upstream manufacturing processes involving mammalian cell substrates. viral and mollicute (mycoplasma sp. and acholeplasma sp.) contamination events have been relatively rare, but many of those that have been reported have been attributed to use of infected animal sera in growth media during cell expansion. the risk of ... | 2011 | 21871817 |
| Contagious Caprine Pleuropneumonia: New Aspects of an Old Disease. | Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. capripneumoniae, is a serious OIE-listed disease affecting goats in the Middle East, north and east Africa and Asia. Mortality and morbidity rates can be as high as 60% and 90%, respectively, when the disease first enters a territory, invariably through carrier animals. Recent detections of CCPP in Pakistan and Tajikistan are probably the result of improved diagnosis as the disease has been suspected there for many ... | 2011 | 21951488 |
| banking human umbilical cord derived mesenchymal stromal cells for clinical use. | a great deal of interest has arisen recently with respect to human mesenchymal stem cells (mscs), due to their broad therapeutic potential. however, the safety and efficacy of mscs expanded ex-vivo for clinical applications remain a concern. in this paper, we establish a standardized process for manufacture of human umbilical cord derived mscs (uc-mscs), which encompasses donor screening and testing, recovery, 2-stage expansion, and administration. the biological properties and safety of uc-mscs ... | 2011 | 21929848 |
| mycoplasma hyopneumoniae transcription unit organization: genome survey and prediction. | mycoplasma hyopneumoniae is associated with swine respiratory diseases. although gene organization and regulation are well known in many prokaryotic organisms, knowledge on mycoplasma is limited. this study performed a comparative analysis of three strains of m. hyopneumoniae (7448, j and 232), with a focus on genome organization and gene comparison for open read frame (orf) cluster (oc) identification. an in silico analysis of gene organization demonstrated 117 ocs and 34 single orfs in m. hyop ... | 2011 | 22086999 |
| comparison of the filmarray respiratory panel and prodesse real-time pcr assays for detection of respiratory pathogens. | we compared the diagnostic performance and overall respiratory pathogen detection rate of the premarket version of the filmarray respiratory panel (rp) multiplex pcr assay (idaho technology, inc., salt lake city, ut) with those of the food and drug administration (fda)-cleared prodesse proflu+, profast+, proparaflu+, pro hmpv+, and proadeno+ real-time pcr assays (gen-probe, san diego, ca). the assays were performed on a panel of 192 nasopharyngeal-secretion specimens collected from 81 children u ... | 2011 | 21998418 |
| feline calicivirus: a neglected cause of feline ocular surface infections? | objective to investigate the prevalence of feline calicivirus (fcv) infection in relation to ocular surface lesions in cats with upper respiratory tract diseases (urtd). animals studied ninety-nine cats with ocular surface infection and symptoms or recent history of urtd were examined at various rescue shelters and hospitals. procedure a complete general and ophthalmic examination was performed including schirmer tear test, slit-lamp biomicroscopy, fluorescein and lissamine green staining. cl ... | 2011 | 22050669 |
| efficacy and safety of a new 450 mg/ml florfenicol formulation administered intramuscularly in the treatment of bacterial bovine respiratory disease. | the objective of the study was the safety and efficacy evaluation of a new 450 mg/ml florfenicol formulation in the treatment of naturally occurring respiratory disease when administered intramuscularly, compared with a positive control group treated with the well-established 300 mg/ml formulation. a total of 174 calves, selected from five sites in france and spain, aged from 1 to 17 months, showing severe signs of respiratory disease, were randomly assigned to treatment with either the 300 mg/m ... | 2011 | 21921060 |
| [clinical report on 68 cases of refractory mycoplasma pneumoniae pneumonia (rmpp)]. | to summarize the early diagnose and treatment approaches of refractory mycoplasma pneumoniae pneumonia (rmpp). | 2011 | 21977599 |
| Variability of Trinucleotide Tandem Repeats in the MgPa Operon and Its Repetitive Chromosomal Elements in Mycoplasma genitalium. | Mycoplasma genitalium, a human pathogen associated with sexually transmitted diseases, is unique in that it has the smallest genome of any known free-living organism. Despite its small genome, 4.7% of the total genomic sequence is devoted to making the MgPa adhesin operon (encoding MG190, MG191 and MG192 genes) and its repetitive chromosomal sequences (known as MgPars). The goals of this study were to investigate the location, organization and variability of trinucleotides tandem repeats (TTRs) ... | 2011 | 21997874 |
| In vitro antimicrobial inhibition of Mycoplasma bovis isolates submitted to the Pennsylvania Animal Diagnostic Laboratory using flow cytometry and a broth microdilution method. | Mycoplasma bovis is an important pathogen of cattle, causing mastitis, pneumonia, conjunctivitis, otitis, and arthritis. Currently there are only a few reports of sensitivity levels for M. bovis isolates from the United States. Mycoplasma bovis isolates submitted to the Pennsylvania Animal Diagnostic Laboratory between December 2007 and December 2008 (n = 192) were tested for antimicrobial susceptibility to enrofloxacin, erythromycin, florfenicol, spectinomycin, ceftiofur, tetracycline, and oxyt ... | 2011 | 21908288 |
| Frequent detection of respiratory agents by multiplex PCR on oropharyngeal samples in Swedish school-attending adolescents. | Abstract Background: Respiratory agents may be detected in the oropharynx of healthy individuals. The extent of this condition and the reasons behind it are largely unknown. The objective of this study was to determine the factors associated with the presence of respiratory agents in the oropharynx of adolescents healthy enough to attend school activities. Methods: On a single day in December, samples from the posterior wall of the oropharynx of adolescents aged 10-15 y were obtained using cot ... | 2011 | 22103467 |
| Adhesion and biofilm formation of Mycoplasma pneumoniae on an abiotic surface. | We demonstrated that when M. pneumoniae was grown on an abiotic surface of either glass or polystyrene with a serum-containing medium, the bacteria adhered to the surface and formed highly differentiated volcano-like biofilm structures. As adherence to the surface and/or biofilm formation was totally inhibited by anti-P1 polyclonal monospecific antibodies, we suggest that the adherence of M. pneumoniae to the abiotic surface and/or biofilm formation is associated with P1, the major tip organelle ... | 2011 | 21879294 |
| development and evaluation of enzyme-linked immunosorbent assay based on recombinant inorganic pyrophosphatase gene antigen for the detection of mycoplasma suis antibodies. | the recombinant ppa protein of mycoplasma suis migrated to 21kda. using this antigen, an elisa system to detect the antibody against m. suis infection in swine was established. the relisa demonstrated 98.5% specificities among negative samples and 96.9% sensitivity among positive samples with m. suis infection. a comparison of this elisa system with an indirect hemagglutination assay (iha) test using 132 swine samples revealed that the positive rate was 34.0% in elisa and 28.0% in iha. compared ... | 2011 | 21940024 |
| mollicutes contamination: a new strategy for an effective rescue of cancer cell lines. | mollicutes contaminations of cellular models can have marked effects on gene expression and cell behaviour in vitro leading to the production of unreliable data, unsafe biopharmaceutical drugs or to the loss of cell culture itself. fortunately, irreplaceable cell culture can be cured by decontamination with the specific antibiotic regimen. here, we describe the treatment of 35 mycoplasma-positive cell lines by the use of the novel antibiotic mycozap(®) as well as evaluate its eradication perform ... | 2011 | 22078664 |
| rising rates of macrolide-resistant mycoplasma pneumoniae in the central united states. | macrolide-resistant mycoplasma pneumoniae is widespread in asia and severe cases of pneumonia have been described in children. little information is available about the resistance pattern in the united states. we collected respiratory samples from 49 patients with mycoplasma infection in the central united states between 2007 and 2010. we found a macrolide resistance rate of 8.2%. resistance should be considered when patients with m. pneumoniae infection do not have a satisfactory response to ma ... | 2011 | 22209916 |
| mycoplasma pneumoniae respiratory tract infections among greek children. | background: m. pneumoniae is a common cause of respiratory tract infections (rtis) of variable severity especially in children. new diagnostic techniques offered more reliable information about the epidemiology of infection by this pathogen.aim: the aim of this study was to investigate the prevalence and epidemiology of acute m. pneumoniae infections among greek children hospitalized for rtis using more advanced techniques.material and methods: the study included 225 greek children hospitalized ... | 2011 | 22110297 |
| an investigation of the pathology of mycoplasma mastitis in the cow. | summary a field case of mastitis in cows, caused by mycoplasma agalactiae var. bovis, formed the occasion to conduct an infection experiment. five lactating heifers were infected in the udder at different times. the cows were slaughtered 2, 5, 7, 9 and 12 days p. i. and the pathological changes were studied. the investigation indicated that the pathological picture differed with time: in the acute stage, the inflammation was characterized by exudation of mostly eosinophils in the alveoli; late ... | 1979 | 22039769 |
| [levels of tnf-α, il-6 and il-10 in bronchoalveolar lavage fluid in children with mycoplasma pneumoniae pneumonia]. | to study the levels and roles of cytokines tnf-α, il-6 and il-10 in bronchoalveolar lavage fluid (balf) in children with mycoplasma pneumoniae pneumonia (mpp). | 2011 | 22000436 |
| severe community-acquired pneumonia caused by macrolide-resistant mycoplasma pneumoniae in a 6-year-old boy. | mycoplasma pneumoniae is the commonest agent causing atypical pneumonia in children. macrolides have long been used in the treatment of community-acquired pneumonia not responsive to beta-lactams alone. in this report, we describe the first locally acquired paediatric patient with severe community-acquired pneumonia caused by macrolide-resistant mycoplasma pneumoniae, possessing an a-to-g transition at position 2063 of the 23s rrna gene. in addition, we have detected two more strains of macrolid ... | 2011 | 21979480 |
| Influences of ORF1 on the virulence and immunogenicity of Actinobacillus pleuropneumoniae. | Actinobacillus pleuropneumoniae is a Gram-negative pathogen that causes porcine pleuropneumonia. The pathogenicity of A. pleuropneumoniae is strongly correlated with the production of active repeat-in-toxin (RTX) proteins such as ApxIVA. We evaluated the contribution of a potential ApxIVA activator, ORF1, to the virulence and immunogenicity of A. pleuropneumoniae in pigs. The orf1 gene in A. pleuropneumoniae SLW03 (serovar 1, ?apxIC?apxIIC) was deleted, producing strain SLW05 (?apxIC?apxIIC?orf1 ... | 2011 | 21964939 |
| Prevalence of Mycoplasma species in bulk tank milk in Japan. | | 2011 | 21921058 |
| RNA polymerase beta subunit (rpoB) gene and the 16S-23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasmataceae. | Conventional classification of the species in the family Mycoplasmataceae is mainly based on phenotypic criteria, which are complicated, can be difficult to measure, and have the potential to be hampered by phenotypic deviations among the isolates. The number of biochemical reactions suitable for phenotypic characterization of the Mycoplasmataceae is also very limited and therefore the strategy for the final identification of the Mycoplasmataceae species is based on comparative serological resul ... | 2012 | 22115576 |
| [a case of acute brainstem and striatal encephalopathy associated with mycoplasma pneumoniae infection]. | acute encephalopathy with bilateral striatal necrosis (ebsn) is a rare reversible neurological disease characterized by an abrupt onset following an acute infectious disease and by severe extra-pyramidal signs associated with striatal lesions. brainstem involvement is rarely observed in this disease. we report a 10-year-old boy who had ebsn associated with mycoplasma pneumoniae. he became lethargic after acute bronchitis. a few days later, he showed extra-pyramidal signs, pyramidal signs and ove ... | 2011 | 22180963 |
| Mycoplasma pneumoniae infection associated with urticarial vasculitis mimicking adult-onset Still's disease. | Mycoplasma pneumoniae is well known to be a frequent cause of atypical pneumonia worldwide. However, it may also present with a wide variety of clinical features, including cutaneous symptoms, which are not widely recognised. Urticarial vasculitis occurring with M. pneumoniae has been described to occur in only one other case report. This amalgamation of non-specific clinical symptoms and signs can lead to a diagnostic dilemma. We describe a case of M. pneumoniae infection presenting with extrap ... | 2011 | 21918897 |
| real-time quantitative polymerase chain reaction detection of haemoplasmas in healthy and unhealthy dogs from central macedonia, greece. | the aims of this study were to determine the prevalence of canine haemoplasmas, mycoplasma haemocanis and "candidatus mycoplasma haematoparvum" infection in central macedonia, greece, and to evaluate any associations between canine haemoplasma infection and clinical presentation, selected laboratory data or the presence of ticks. | 2011 | 22017540 |
| Antibiotic Sensitivity of 40 Mycoplasma pneumoniae Isolates and Molecular Analysis of Macrolide Resistant Isolates from Beijing, China. | Minimum inhibitory concentrations of eight antibiotics were detected with 40 Chinese Mycoplasma pneumoniae isolates. Thirty-eight isolates (95%) were macrolide resistant. Each macrolide resistant isolate harbored an A2063G or A2064G point mutation in the 23S rRNA gene. All 40 isolates (100%) were type I strains, but they might have originated from different clones. | 2011 | 22106216 |
| [Effects of the symbiosis of Trichomonas vaginalis with Mycoplasma hominis on ferredoxin gene]. | We isolated 30 Trichomonas vaginalis for the PCR detection from the gynecological outpatients in the Affiliated Hospital of Zhengzhou University using the specific 16s rDNA primers of Mycoplasma hominis. The results showed that there were 25 cases of Mycoplasma hominis infection, with the infection rate of 83.33%. This gave a clew that the symbiosis of Trichomonas vaginalis with Mycoplasma hominis may be of certain generality in China. We sequenced the ferredoxin gene of 10 Trichomonas vaginalis ... | 2011 | 21936384 |
| Identification of a Mycoplasma ovis-like organism in a herd of farmed white-tailed deer (Odocoileus virginianus) in rural Indiana. | Mycoplasma ovis is a hemoplasma parasite of sheep, goats, and reindeer; however, natural hemoplasma infection in white-tailed deer has not previously been reported. Subsequent to finding many coccoid, bacillary, and ring-shaped organisms, consistent with hemotropic mycoplasmas, on RBCs from a 72-day-old female white-tailed fawn, we sought to (1) identify the putative hemoplasma observed in blood from the fawn, (2) evaluate others in the herd for hemoplasma infection, and (3) identify clinicopath ... | 2011 | 22112325 |
| evidence of acute mycoplasma infection in a patient with incomplete and atypical kawasaki disease: a case report. | the etiology of kawasaki disease remains unknown despite extensive studies. some researchers suggest that it is caused by an infectious agent. this is a case report where a patient with incomplete kawasaki disease was found to have evidence compatible with acute mycoplasma pneumoniae infection. this is one of the several case reports linking mycoplasma pneumoniae to kawasaki disease as a possible trigger. this is perhaps due to a superantigen or is mediated by some other mechanism. accurate and ... | 2011 | 22203852 |
| bacterial vaginosis: what is physiological in vaginal bacteriology? an update and opinion. | bacterial vaginosis (bv) was originally described as a sexually transmitted infection caused by a single microbe. the prevailing concept describes bv as a polymicrobial coitus-associated disease of uncertain origin. in this overview the natural history of bv as a monobacterial and polymicrobial entity is examined with respect to the physiological concept of bv in relation to sexual behavior before and after introduction of the hormonal contraceptive pill. bacterial vaginosis, characterized by lo ... | 2011 | 21916857 |
| Characterization of the antigenic and functional domains of a Mycoplasma synoviae variant vlhA gene. | The Mycoplasma synoviae haemagglutinin gene, vlhA, encodes two major immunodominant and surface-exposed membrane proteins, MSPB and MSPA. Both products are antigenically variable but only MSPA mediates binding to erythrocytes. Previously we have shown that M. synoviae type strain WVU 1853 could express a variant vlhA gene, referred to as MS2/28.1, with a considerably shorter and divergent MSPA region. A finding that prompted detailed characterization of its antigenic and functional properties. H ... | 2011 | 22176762 |
| [Streptococcal toxic shock syndrome or Kawasaki disease? Two case studies of children with group A streptococcal pneumonia empyema]. | We report 2 cases of children with group A streptococcus pyogenes pleuropneumonia, in one child associated with Kawasaki disease and in the other with streptococcal toxic shock syndrome. These 2 features, with theoretically well-defined clinical and biological criteria, are difficult to differentiate in clinical practice, however, likely due to their pathophysiological links. In case of clinical doubt, an echocardiography needs to be performed to search for coronary involvement and treatment inc ... | 2011 | 22041597 |
| Functional characterization of the RuvB homologs from Mycoplasma pneumoniae and Mycoplasma genitalium. | Homologous recombination between repeated DNA elements in the genomes of Mycoplasma species has been hypothesized to be a crucial causal factor in sequence variation of antigenic proteins at the bacterial surface. To investigate this notion, studies were initiated to identify and characterize the proteins that form part of the homologous DNA recombination machinery in Mycoplasma pneumoniae as well as Mycoplasma genitalium. Among the most likely participants of this machinery are homologs of the ... | 2011 | 21949077 |
| Hot topic: Bovine milk samples yielding negative or nonspecific results in bacterial culturing-The possible role of PCR-single strand conformation polymorphism in mastitis diagnosis. | A large proportion of mastitis milk samples yield negative or nonspecific results (i.e., no mastitis pathogen can be identified) in bacterial culturing. Therefore, the culture-independent PCR-single strand conformation polymorphism method was applied to the investigation of bovine mastitis milk samples. In addition to the known mastitis pathogens, the method was suitable for the detection of fastidious bacteria such as Mycoplasma spp., which are often missed by conventional culturing methods. Th ... | 2012 | 22192188 |
| egg production in relation to the results of a long term serological survey of 73 flocks of fowl. | summary seventy-three flocks of fowl were tested at regular intervals for the presence of precipitins to fowl adenovirus (av) and infectious bronchitis virus (ibv), haem-agglutinating inhibiting antibodies to bc14 virus, and of agglutinins to mycoplasma gallisepticum (m.g.) and mycoplasma synoviae (m.s.). in all the eight flocks affected with egg drop syndrome (eds '76), egg production problems were associated with increasing numbers of bci4 virus reactors and av reactors. in flocks showing p ... | 1980 | 22039854 |
| Potential role of Mycoplasma hominis in interleukin (IL)-17-producing CD4+ T-cell generation via induction of IL-23 secretion by human dendritic cells. | Mycoplasma hominis, a human urogenital pathogen, is involved in genital and extragenital infections and arthritis, particularly in immunocompromised patients. The interleukin (IL) 23/T helper (Th) 17 axis is associated with inflammatory and autoimmune diseases. The aim of this study was to assess the IL-23 response to M. hominis in human dendritic cells (DCs) and the CD4(+) T-cell differentiation in response to M. hominis-infected DCs. | 2011 | 21971287 |
| A new variant of Actinobacillus pleuropneumoniae serotype 3 lacking the entire apxII operon. | Actinobacillus pleuropneumoniae is an important respiratory pathogen causing pleuropneumonia in pig. The species is genetically characterized by the presence of 4 RTX (Repeats in the Structural ToXin) toxin genes: apxI, apxII, and apxIII genes are differentially present in various combinations among the different serotypes, thereby defining pathogenicity; the apxIV gene is present in all serotypes. Polymerase chain reaction (PCR)-based apx gene typing is done in many veterinary diagnostic labora ... | 2011 | 21908290 |
| Sequence TTKF ? QE defines the site of proteolytic cleavage in Mhp683 protein, a novel glycosaminoglycan and cilium adhesin of Mycoplasma hyopneumoniae. | Mycoplasma hyopneumoniae colonizes the ciliated respiratory epithelium of swine, disrupting mucociliary function and inducing chronic inflammation. P97 and P102 family members are major surface proteins of M. hyopneumoniae and play key roles in colonizing cilia via interactions with glycosaminoglycans and mucin. The p102 paralog, mhp683, and homologs in strains from different geographic origins encode a 135-kDa pre-protein (P135) that is cleaved into three fragments identified here as P45(683), ... | 2011 | 21969369 |
| Liquid-based urine cytology as a tool for detection of human papillomavirus, Mycoplasma, and Ureaplasma in men. | Background: Liquid-based urine cytology (LB-URC) was evaluated for cytological diagnosis and detection of human papillomavirus (HPV), Mycoplasma, and Ureaplasma.Methods: Midstream urine samples were collected from 141 male patients with urethritis and 154 controls without urethritis, and sediment cells were preserved in liquid-based cytology solution. Urethral swabs from urethritis patients were tested for Neisseria gonorrhoeae and Chlamydia trachomatis. Papanicolaou tests were performed for cyt ... | 2011 | 22135257 |
| the genome of th17 cell-inducing segmented filamentous bacteria reveals extensive auxotrophy and adaptations to the intestinal environment. | perturbations of the composition of the symbiotic intestinal microbiota can have profound consequences for host metabolism and immunity. in mice, segmented filamentous bacteria (sfb) direct the accumulation of potentially proinflammatory th17 cells in the intestinal lamina propria. we present the genome sequence of sfb isolated from monocolonized mice, which classifies sfb phylogenetically as a unique member of clostridiales with a highly reduced genome. annotation analysis demonstrates that sfb ... | 2011 | 21925113 |
| fatal outbreak of mycoplasma capricolum pneumonia in endangered markhors. | a pneumonia outbreak reduced the numbers of a wild population of endangered markhors (capra falconeri) in tajikistan in 2010. the infection was diagnosed by histologic examination and bacteriologic testing. mycoplasma capricolum subsp. capricolum was the sole infectious agent detected. cross-species transmission from domestic goats may have occurred. | 2011 | 22172532 |
| Contagious bovine pleuropneumonia: humoral and pathological events in cattle infected by endotracheal intubation or by exposure to infected animals. | Results of trials in which cattle were infected by endotracheal intubation of Mycoplasma mycoides var. mycoides small colony (MmmSC) cultures or by contact exposure to animals affected by contagious bovine pleuropneumonia (CBPP) are numerous. However, an analysis of the effects of the two different routes of infection on disease outcome is lacking. This study analyses the disease outcome in cattle infected by the two methodologies. Data originate from two controlled trials conducted in Namibia u ... | 2011 | 22194224 |
| survival of bighorn sheep (ovis canadensis) commingled with domestic sheep (ovis aries) in the absence of mycoplasma ovipneumoniae. | to test the hypothesis that mycoplasma ovipneumoniae is an important agent of the bighorn sheep (ovis canadensis) pneumonia that has previously inevitably followed experimental commingling with domestic sheep (ovis aries), we commingled m. ovipneumoniae-free domestic and bighorn sheep (n=4 each). one bighorn sheep died with acute pneumonia 90 days after commingling, but the other three remained healthy for >100 days. this unprecedented survival rate is significantly different (p=0.002) from that ... | 2012 | 22247385 |
| genome sequence for "candidatus mycoplasma haemominutum," a low-pathogenicity hemoplasma species. | we present the genome sequence of "candidatus mycoplasma haemominutum" strain birmingham 1, a low-pathogenicity feline hemoplasma strain. | 2012 | 22275103 |
| persistently elevated iga antibodies to mycoplasma pneumoniae in patients with internal carotid artery stenosis. | background: it has been suggested that mycoplasma pneumoniae may play a role in the development of atherosclerosis, but to date this association is still a matter of debate due to conflicting findings.methods: we have investigated the levels of specific iga antibodies to m. pneumoniae in 91 patients with internal carotid artery (ica) stenosis using a commercial kit (seromp™ iga; savyon diagnostics, israel; cut-off value: 20 binding units; bu). all patients underwent surgery for ica stenosis. fro ... | 2011 | 22242085 |
| generation of a monoclonal antibody against mycoplasma following accidental contamination during production of a monoclonal antibody against lawsonia intracellularis. | this report describes mycoplasma contamination of lawsonia intracellularis cultures that led to the unintended acquisition of a monoclonal antibody against mycoplasma during the attempted generation of a monoclonal antibody against l. intracellularis. | 2012 | 22247145 |
| [molecular typing and immunoblotting of 17 mycoplasma ovipneumoniae isolates from china]. | to study the heterogeneity and immunogenic variability among mycoplasma ovipneumoniae (m. ovipneumoniae) isolates from different regions of china. | 2011 | 22233065 |
| [effects of two antigens for igm detection of toxoplasmosis]. | to observe the effects of recombinant toxoplasma gondii p30 antigen and soluble antigen for igm detection of toxoplasmosis. | 2011 | 22263518 |
| type iv fimbrial subunit protein apfa contributes to protection against porcine pleuropneumonia. | abstract: porcine pleuropneumonia caused by actinobacillus pleuropneumoniae accounts for serious economic losses in the pig farming industry worldwide. we examined here the immunogenicity and protective efficacy of the recombinant type iv fimbrial subunit protein apfa as a single antigen vaccine against pleuropneumonia, or as a component of a multi-antigen preparation comprising five other recombinant antigens derived from key virulence factors of a. pleuropneumoniae (apxia, apxiia, apxiiia, apx ... | 2012 | 22240397 |
| increased prevalence of torque teno viruses in porcine respiratory disease complex affected pigs. | the role of swine torque teno sus viruses (ttsuvs) as co-factors in disease syndromes involving porcine circovirus strain 2 (pcv2) and porcine reproductive and respiratory disease syndrome virus (prrsv) has been a debatable subject. in this study, the prevalence of ttsuvs in iowa, the leading pork producing state in the u.s., was estimated by a duplex pcr. the pcr is capable of simultaneously detecting both teno sus viruses 1 and 2 (ttsuv1 and 2). based on an analysis of 300 random samples repre ... | 2011 | 22227415 |
| reactive oxygen species mediate jak2/stat3 activation and il-8 expression in pulmonary epithelial cells stimulated with lipid-associated membrane proteins from mycoplasma pneumoniae. | objective: to investigate the involvement of reactive oxygen species (ros) in the activation of janus kinase2 (jak2)/signal transducers and activators of transcription3 (stat3), and il-8 expression in pulmonary epithelial cells stimulated with lipid-associated membrane proteins (lamp) from mycoplasma pneumoniae using a known antioxidant, n-acetylcysteine (nac). methods: pulmonary epithelial a549 cells were treate ... | 2012 | 22270622 |
| effect of challenge of pigs previously immunised with inactivated vaccines containing homologous and heterologous mycoplasma hyopneumoniae strains. | abstract: background: mycoplasma hyopneumoniae is the primary cause of enzootic pneumonia in pigs. although vaccination is an important control tool, the results observed under field conditions are variable. this may be due to antigenic differences between the strains circulating in pig herds and the vaccine strain. this study compared the protective efficacy of four bacterins against challenge infection with a highly virulent field strain of m. hyopneumoniae. seventy eight, one-week old pigle ... | 2012 | 22225838 |
| development of a rotor-gene real-time pcr assay for the detection and quantification of mycoplasma genitalium. | we developed and validated a real-time quantitative polymerase chain reaction (qpcr) assay to determine mycoplasma genitalium bacterial load in endocervical swabs, based on amplification of the pdhd gene which encodes dihydrolipoamide dehydrogenase, using the rotor-gene platform. we first determined the qpcr assay sensitivity, limit of detection, reproducibility and specificity, and then determined the ability of the qpcr assay to quantify m. genitalium in stored endocervical specimens collected ... | 2011 | 22230235 |
| potential infectious etiology of behçet's disease. | behçet's disease is a multisystem inflammatory disorder characterized by recurrent oral aphthous ulcers, genital ulcers, uveitis, and skin lesions. the cause of behçet's disease remains unknown, but epidemiologic findings suggest that an autoimmune process is triggered by an environmental agent in a genetically predisposed individual. an infectious agent could operate through molecular mimicry, and subsequently the disease could be perpetuated by an abnormal immune response to an autoantigen in ... | 2012 | 22254152 |
| treatment of acute pelvic inflammatory disease. | pelvic inflammatory disease (pid), one of the most common infections in nonpregnant women of reproductive age, remains an important public health problem. it is associated with major long-term sequelae, including tubal factor infertility, ectopic pregnancy, and chronic pelvic pain. in addition, treatment of acute pid and its complications incurs substantial health care costs. prevention of these long-term sequelae is dependent upon development of treatment strategies based on knowledge of the mi ... | 2011 | 22228985 |
| [mycoplasma pneumoniae encephalitis associated with basal ganglia necrosis.] | introduction: severe central nervous system diseases, such as encephalitis, have been reported in association with mycoplasma pneumoniae infections. case report: a previously healthy 5-year-old boy presented with an atypical pneumonia; he rapidly developed encephalitis revealed by lethargy, generalized status epilepticus. mri showed abnormal signals in the basal ganglia, typical of bilateral striatal necrosis. serologic tests for m. pneumoniae were positive, the child recovered almost completely ... | 2012 | 22244792 |
| erratum to: caprine pleuropneumonia in beetal goats. | | 2012 | 22231018 |