Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| mycobacterium heckeshornense tenosynovitis. | we describe the first case of tenosynovitis due to mycobacterium heckeshornense, a mycobacterium characterized in 2000 and only incriminated in a few previous cases of infections. molecular identification of this pathogen included 16s rrna and hsp65 gene sequencing. m. heckeshornense may cause a wide spectrum of human infectious diseases and may be underestimated due to its phenotypic relatedness with mycobacterium xenopi. | 2006 | 17148085 |
| genetic basis for natural and acquired resistance to the diarylquinoline r207910 in mycobacteria. | the atpe gene encoding the subunit c of the atp synthase of mycobacterium tuberculosis, the target of the new diarylquinoline drug r207910, has been sequenced from in vitro mutants resistant to the drug. the previously reported mutation a63p and a new mutation, i66m, were found. the genetic diversity of atpe in 13 mycobacterial species was also investigated, revealing that the region involved in resistance to r207910 is conserved, except in mycobacterium xenopi in which the highly conserved resi ... | 2006 | 16870785 |
| successful management of two heart transplant recipients with mycobacterial pulmonary infections. | we report two cases of pulmonary tuberculosis in heart transplant recipients: a 46-year-old man with pulmonary tuberculosis due to mycobacterium tuberculosis and a 64-year-old man with nontuberculous mycobacterial infection with pulmonary infiltrates due to mycobacterium xenopi. the time intervals from transplantation to diagnosis were 3 and 4 years, respectively. the patient with tuberculosis underwent standard treatment with isoniazid, rifampin, ethambutol, and pyrazinamide. the patient with t ... | 2005 | 16039241 |
| species-specific identification of mycobacterial 16s rrna pcr amplicons using smart probes. | due to growing problems with new emerging pathogens, cost-effective and manageable methods for their accurate identification in routine diagnostics are urgently required. of particular importance is the genus mycobacterium with its more than 100 species. identification of these species is hampered by their slow growth in the laboratory and by the obligate need for dna sequence analysis. to provide a fast and reliable diagnostic tool, we developed a novel approach using fluorescently labeled dna ... | 2005 | 16285666 |
| completion pneumonectomy for chronic mycobacterial disease. | patients with persistent pulmonary infections from mycobacterial disease present a difficult clinical challenge. these individuals typically have poor pulmonary function, malnutrition, and other comorbidities, and few guidelines exist regarding optimal therapy. we report our experience with completion pneumonectomy as part of a multidisciplinary treatment program for patients with recurrent, persistent mycobacterial disease. | 2005 | 15942565 |
| spondylitis due to mycobacterium xenopi in a human immunodeficiency virus type 1-infected patient: case report and review of the literature. | nontuberculous mycobacterial infections are well known to occur in patients with human immunodeficiency virus infection. however, spondylitis due to mycobacteria other than mycobacterium tuberculosis is uncommon. we report a case of biopsy- and culture-proven mycobacterium xenopi spondylitis in an aids patient and discuss approaches to diagnosis and therapy. this case serves to highlight the potential pathogenic role of this usually environmental commensal organism in severely immunosuppressed a ... | 2005 | 15750135 |
| development and evaluation of a mycobacterium avium subspecies paratuberculosis (map) specific multiplex pcr assay. | there are specificity questions inherent in most of the current pcr systems that amplify the map is900 sequence as an indicator for mycobacterium avium subspecies paratuberculosis (map) presence due to false positives associated with is900-like sequences that exists in other mycobacterium species besides map. we developed a multiplex pcr system designed to enhance specificity for map detection in a single pcr reaction. this pcr assay co-amplifies the mycobacterium species 16s rrna gene, map is90 ... | 2005 | 15982769 |
| [exclusive surgical treatment of mycobacterium xenopi infection of a pulmonary cavity]. | the diagnosis of pulmonary infection due to mycobacterium xenopi was retained in a 48-year-old immunocompetent patient. the lesion was isolated in a peripheral region and was well limited. exclusive surgical treatment was undertaken. cure was complete without recurrence at three years. this case illustrates that in certain cases environmental mycobacteriosis with a very limited lesion may respond very well to surgical resection, an interesting alternative to medication. | 2005 | 16208190 |
| [molecular identification of mycobacteria and detection of antibiotic resistance]. | mycobacteria are responsible for many human infections, especially species of tuberculosis complex, causative agents of tuberculosis. with nine millions new cases every year, this disease is responsible for more than two millions of deaths. nontuberculous mycobacteria (e.g. mycobacterium avium-intracellulare, mycobacterium kansasii, mycobacterium xenopi or mycobacterium ulcerans) can cause infections too, usually in particular clinical settings. standard diagnosis of mycobacterial infections rel ... | 2004 | 15297234 |
| mycobacterium xenopi infection after heart transplantation: an unreported pathogen. | mycobacterial infections are a well-known, potentially serious, albeit infrequent complication of solid-organ transplantation. nontuberculous mycobacteria generally account for less than 50% of all such isolates in this patient population. mycobacterium xenopi, an environmentally ubiquitous organism and common contaminant of hospital hot water systems, is a particularly uncommon isolate after transplantation and has never been reported in heart allograft recipients. we report the occurrence of c ... | 2004 | 15621162 |
| [metastatic endophthalmitis following lung transplantation]. | intraocular septic--bacterial, viral or fungal--metastasis originating from an infectious focus is very rare but serious complication. the authors performed one enucleation and one evisceration on two patients because of endophthalmitis secondary to pulmonary infection following lung transplantation. the pathogens were pseudomonas aeruginosa and mycobacterium xenopi respectively. with this case report the authors aim to draw attention to a rare, but serious ophthalmic complication of organ trans ... | 2004 | 15462477 |
| mycobacterium xenopi pulmonary infection in two renal transplant recipients under sirolimus therapy. | opportunistic infection is a major threat to immunocompromised patients. however, infection due to mycobacterium xenopi is rare in renal transplant recipients. we report two new cases of m. xenopi pulmonary infection (one case of interstitial pneumopathy and one of a pulmonary nodule) in renal transplant recipients, detected in the same center at an interval of a few months. both patients were on an immunosuppressive regimen including a recent switch to sirolimus. sirolimus is a new immunosuppre ... | 2004 | 15762936 |
| a decade surveillance study of mycobacterium xenopi disease and antimicrobial susceptibility levels in a reference teaching hospital of northern italy: hiv-associated versus non-hiv-associated infection. | the aim of our survey is to investigate the epidemiology and in vitro antimicrobial susceptibility levels of 35 consecutive mycobacterium xenopi strains responsible for confirmed disease at a university hospital from 1993 to 2002 and to identify eventual differences in the in vitro sensitivity profile between the 17 strains isolated from patients with hiv disease and the 18 isolates cultured from non-hiv-infected individuals. | 2004 | 15472795 |
| detection of mycobacterial dna from sputum of patients with cystic fibrosis. | patients with cystic fibrosis (cf) are at high risk from atypical mycobacterial infections. there have been few attempts to delineate the intensity of mycobacterial infection in cf patients in ireland. | 2004 | 15540712 |
| [four cases of mycobacterium xenopi pulmonary disease]. | mycobacterium xenopi is very rare pathogen in japan. we reported herein four cases of m. xenopi pulmonary disease. these patients were all male and their ages ranged from 53 to 72. they all had past history of pulmonary tuberculosis, including two cases who had been also treated for mycobacterium kansasii pulmonary disease later. the bacilli could be cultured in mycobacteria growth indicator tube (mgit) system from 10 sputum samples, but they could not be cultured on ogawa egg media except for t ... | 2004 | 15168439 |
| novel mass spectrometry-based tool for genotypic identification of mycobacteria. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) after base-specific cleavage of pcr amplified and in vitro-transcribed 16s rrna gene (rdna) was used for the identification of mycobacteria. full-length 16s rdna reference sequences of 12 type strains of mycobacterium spp. frequently isolated from clinical specimens were determined by pcr, cloning, and sequencing. for maldi-tof ms-based comparative sequence analysis, mycobacterial 16s rdna signature seque ... | 2004 | 14715774 |
| recurrent mycobacterium xenopi infection in a patient with rheumatoid arthritis receiving etanercept. | a case of recurrent mycobacterium xenopi infection presenting as pott's disease in a patient receiving etanercept for severe rheumatoid arthritis is described. a 49-y-old caucasian male had received a total of 11 months of anti-mycobacterial therapy for hip infection acquired 15 months earlier; he presented with progressive back pain, which was diagnosed as pott's disease. he had been treated with etanercept in addition to his prior immunosuppressive agents after the diagnosis of hip infection. | 2004 | 15061673 |
| clinical and therapeutic management of pulmonary mycobacterium xenopi infection in hiv-positive patients. | 2004 | 15156459 | |
| mycobacterium xenopi infection in an immunosuppressed patient with crohn's disease. | a 48 year old patient with active crohn's disease presented with bilateral nodules over his lungs resembling malignant metastasis. bronchoscopic and pathological examination of the airways and sputum did not show any malignancy. after 6 weeks mycobacterium xenopi was cultured from his bronchial washings while all other cultures remained negative. treatment was started with rifampicin, ethambutol, and clarithromycin and, after 9 months of treatment, there was an almost complete resolution of his ... | 2004 | 15223876 |
| concurrent granulomatous pneumocystis carinii and mycobacterium xenopi pneumonia: an unusual manifestation of hiv immune reconstitution disease. | this report of concurrent granulomatous pneumocystis carinii pneumonia (gpcp) and mycobacterium xenopi pneumonia (mxip) in a patient with advanced hiv disease 3-5 weeks after commencing antiretroviral therapy (art) fits the syndrome of hiv immune reconstitution/restoration disease (ird). it may also be a unique window into the immunology of granulomatous inflammation. | 2004 | 15516479 |
| non-tuberculous mycobacteria: patterns of isolation. a multi-country retrospective survey. | to collect data on non-tuberculous mycobacteria (ntm) isolated from clinical laboratories in different countries to establish: 1) whether the isolation of ntm was increasing, 2) which species were increasing, and 3) whether there was any pattern of geographical distribution. | 2004 | 15527150 |
| isolation of non-tuberculous mycobacteria from hospital cockroaches (periplaneta americana). | this study attempted to isolate mycobacteria from hospital and household cockroaches from 90 hospitals and 40 households in kaohsiung city and kaohsiung county, south taiwan. among 203 cockroaches (139 periplaneta americana and 64 blattella germanica) collected from the hospitals, six mycobacterium spp. were isolated and identified by polymerase chain reaction-restriction fragment length polymorphism analysis. in 12 cockroaches (p. americana): four mycobacterium kansaii, three mycobacterium xeno ... | 2003 | 12623325 |
| in vitro activity of linezolid against slowly growing nontuberculous mycobacteria. | mics of linezolid in broth microdilutions were tested against 341 slowly growing nontuberculous mycobacteria (ntm) belonging to 15 species. the proposed linezolid susceptibility mics for all mycobacterium marinum, mycobacterium szulgai, mycobacterium kansasii, mycobacterium malmoense, and mycobacterium xenopi isolates and for 90% of mycobacterium gordonae and mycobacterium triplex isolates were </=8 micro g/ml. linezolid has excellent therapeutic potential against most species of ntm. | 2003 | 12709349 |
| identification of three strains of mycobacterium species isolated from clinical samples using fatty acid methyl ester profiling. | the cellular fatty acid profiles of 67 strains belonging to three different species of the genus mycobacterium were determined by gas chromatography of the fatty acid methyl esters, using the midi sherlock microbial identification system (mis). the species m. tuberculosis, m. xenopi and m. avium complex were clearly distinguishable and could be identified based on the presence and concentrations of 12 fatty acids: 14:0, 15:0, 16:1 omega 7c, 16:1 omega 6c, 16:0, 17:0, 18:2 omega 6.9c, 18:1 omega ... | 2003 | 12760317 |
| an unusual cause for a colonic stricture: a case of mycobacterium xenopi mimicking malignancy. | colonic strictures are very common and are a frequent finding on double contrast barium examination; they very often reflect the presence of malignancy, diverticular disease, ischaemia, inflammatory bowel disease, and rarely mycobacterial disease. a case of mycobacterial disease mimicking malignancy is presented; this was due to a rare mycobacterial subtype and there was complete resolution of symptoms with antimycobacterial therapy. | 2003 | 14707250 |
| mycobacterium xenopi pneumonia in the southeastern united states. | mycobacterium xenopi (m. xenopi) is a slow-growing, nontuberculous mycobacterium (ntm). this organism is found in fresh water and has been isolated in water samples collected from water systems in homes and hospitals. before the acquired immunodeficiency syndrome epidemic, m. xenopi infection was infrequent and occurred in clusters; however, m. xenopi is now a recognized cause of pulmonary infection in immunocompetent patients with preexisting lung disease. the classic chest x-ray appearance is ... | 2003 | 12938788 |
| pulmonary mycobacterium xenopi infection in aids patients treated with haart in hungary. | reported here are three cases of pulmonary mycobacterium xenopi infection that occurred in aids patients in hungary shortly after starting highly active antiretroviral therapy. in this country, mycobacterium xenopi is the most common nontuberculous mycobacterial species causing pulmonary mycobacterial infections. cases of pulmonary mycobacterium xenopi disease have been described in patients infected with the human immunodeficiency virus infection and in patients with other immunodeficiencies; h ... | 2003 | 14557921 |
| psoas abscess due to mycobacterium xenopi infection. | 2003 | 12637145 | |
| pulmonary disease caused by mycobacterium xenopi in hiv-negative patients: five year follow-up of patients receiving standardised treatment. | the literature concerning the management of pulmonary disease caused by mycobacterium xenopi is scanty and consists of retrospective reports, mostly of small series of patients. our aim was to document the clinical features and response to treatment of this rare but challenging disease. patients were treated in a randomised, multi-centre trial with either rifampicin plus ethambutol or rifampicin, ethambutol and isoniazid. clinical, bacteriological and radiological progress was monitored at set i ... | 2003 | 12693807 |
| usefulness of a new mycobacteriophage-based technique for rapid diagnosis of pulmonary tuberculosis. | a new mycobacteriophage-based technique (phagetek mb) was compared with standard culture and staining techniques for diagnosis of pulmonary tuberculosis. a total of 2,048 respiratory specimens from 1,466 patients collected from february 2000 to march 2001 were studied by both (i) conventional methods (direct microscopic examination [auramine-rhodamine fluorochrome], and culture in bact/alert 3d and solid media) and (ii) the phagetek mb assay. this phenotypic test utilizes specific mycobacterioph ... | 2003 | 12843014 |
| role of mycobacterium xenopi disease in patients with hiv infection at the time of highly active antiretroviral therapy (haart). comparison with the pre-haart period. | a reliable and timely clinical, radiological, and bacteriological diagnosis, and an optimal treatment of non-tubercular mycobacteriosis (including mycobacterium xenopi disease), remain an unanswered challenge for clinicians facing immunocompromised patients, including those with hiv infection. | 2003 | 12972345 |
| [two cases of pulmonary mycobacterium xenopi infection]. | we report on two patients diagnosed as having mycobacterium xenopi infections. case 1 occurred in a 40-year-old man. his chest radiograph revealed a cavitary lesion with a granular shadow in the right upper lung field. his sputum and bronchial lavage were negative for acid-fast bacilli and malignant cells. for a definitive diagnosis, lung resection was performed by video-assisted thoracoscopy. case 2 was in a 45-year-old man. his chest radiograph showed a cavitary lesion with infiltration in the ... | 2003 | 14503343 |
| human immunodeficiency virus type 1-related pulmonary mycobacterium xenopi infection: a need to treat? | we report treatment decisions and outcomes for 20 patients who were infected with human immunodeficiency virus type 1 (hiv-1) and were receiving highly active antiretroviral therapy (haart) who had respiratory symptoms and from whom mycobacterium xenopi was isolated. all patients also had coexisting pulmonary pathologic conditions. the median blood t cell cd4 count was 37 cells/microl (range, 2-480 cells/microl). fifteen of 20 patients received no antimycobacterial therapy and remain healthy aft ... | 2003 | 14557971 |
| mycobacterium xenopi and drinking water biofilms. | the ability of mycobacterium xenopi to colonize an experimental drinking water distribution system (a propella reactor) was investigated. m. xenopi was present in the biofilm within an hour following its introduction. after 9 weeks, it was always present in the outlet water (1 to 10 cfu 100 ml(-1)) and inside the biofilm (10(2) to 10(3) cfu cm(-2)). biofilms may be considered reservoirs for the survival of m. xenopi. | 2003 | 14602661 |
| [lung tissue destructive lesions caused by nontuberculous mycobacteria]. | forty-two patients with pulmonary mycobacteriosis were clinically characterized. in most patients, mycobacteriosis developed in the presence of residual changes in the lungs after cured tuberculosis. the torpid onset of mycobacteriosis with a gradual augmentation of symptoms of intoxication was observed. all the examinees were found to have destructive lung tissue changes that were characterized by the presence of caverns with a significantly transformed lung pattern. | 2003 | 14689791 |
| [pulmonary mycobacterioses--frequency of occurrence, clinical spectrum and predisposing factors]. | various types of non-tuberculous mycobacteria can be the aetiologic factors of chronic lung infections especially in patients with underlying chronic lung diseases. the aim of this study is to present the cases of pulmonary mycobacterioses observed in institute of tuberculosis and lung diseases in the years 1995-2001. there were 23 patients, 12 men and 11 women in the age between 35-77 years, mean 56 years. 16 out of 23 patients had underlying respiratory problems, mainly healed tuberculosis (7) ... | 2002 | 12884564 |
| development of a positive genetic selection system for inhibition of protein splicing using mycobacterial inteins in escherichia coli dna gyrase subunit a. | an intein-based positive genetic selection system was developed to study protein splicing and to provide a selection system with the potential for finding splicing inhibitors. inteins can be novel antimicrobial targets when present in essential proteins since blocking splicing would kill the organism. for example, pathogenic mycobacteria encode inteins that interrupt dna gyrase. the gyrase selection system exploits (1) splicing of inteins out of gyrase a and (2) the dominant lethal effect of qui ... | 2002 | 12432958 |
| mycobacterium xenopi. | 2002 | 12096215 | |
| evaluation of a commercial line probe assay for identification of mycobacterium species from liquid and solid culture. | the performance of a commercial line probe assay (lipa) (inno-lipa mycobacteria; innogenetics, belgium) for the detection and identification of mycobacterium species from liquid and solid culture was evaluated at five routine clinical laboratories. the lipa method is based on the reverse hybridization principle, in which the mycobacterial 16s-23s ribosomal rna (rrna) spacer region is amplified by polymerase chain reaction (pcr). amplicons are subsequently hybridized with oligonucleotide probes a ... | 2002 | 12461589 |
| in vitro sensitivity of mycobacterium xenopi to five antibiotics. | we tested 20 strains of mycobacterium xenopi (m. xenopi) in order to evaluate their in vitro sensitivity to amikacine, clarithromycine, ethambutol, ofloxacine and rifampicin, by establishing minimal inhibitory concentration (mic) on agar medium. mics of amikacine, clarithromycine and ofloxacine are low, so that these antibiotics can be used in the treatment of m. xenopi infections. mics of ethambutol are higher than seric concentrations. though, its therapeutic use is due to its in vivo ability ... | 2002 | 12504367 |
| [relative relatedness of 11 mycobacteria species by microplate hybridization methods considering delta tm]. | intra-species variance within mycobacterium xenopi, mycobacterium gordonae or mycobacterium szulgai has been reported in identification employing chemotaxonomic characteristics, 16 s rrna gene sequences or relative relatedness (relative color index) of genomic dna-dna hybridization. genomic dna-dna reassociation at the constant temperature was found to be unreliable for classification of mycobacterial species. however, nonspecific dna reassociation could be avoided by hybridization at 56 degrees ... | 2002 | 12235846 |
| creation of an artificial bifunctional intein by grafting a homing endonuclease into a mini-intein. | the majority of inteins are comprised of a protein splicing domain and a homing endonuclease domain. experimental evidence has demonstrated that the splicing domain and the endonuclease domain in a bifunctional intein are largely independent of each other with respect to both structure and activity. here, an artificial bifunctional intein has been created through the insertion of an existing homing endonuclease into a mini-intein that is naturally lacking this functionality. the gene for i-crei, ... | 2002 | 12381313 |
| mycobacterium xenopi pulmonary infection in an hiv infected patient under highly active antiretroviral treatment. | highly active antiretroviral therapy (haart) is responsible for a striking reduction in aids related morbidity and mortality by partly restoring immune function. however, haart can also precipitate the development of clinically apparent opportunistic infections in patients with latent infections. we report a case of an hiv infected patient who developed granulomatous nodular and cavitatory lesions of the lungs due to mycobacterium xenopi as a manifestation of the immune restoration syndrome. | 2002 | 11872863 |
| establishment of intein-mediated protein ligation under denaturing conditions: c-terminal labeling of a single-chain antibody for biochip screening. | intein-mediated protein ligation is a recently developed method that enables the c-terminal labeling of proteins. this technique requires a correctly folded intein mutant that is fused to the c-terminus of a target protein to create a thioester, which allows the ligation of a peptide with an n-terminal cysteine (1, 2). here we describe the establishment of this method for the labeling, under denaturing conditions, of target proteins that are expressed insolubly as intein fusion proteins. a gfpuv ... | 2002 | 12121124 |
| [mycobacterium xenopi: a rare mycobacterium?]. | 2002 | 12199926 | |
| [nontuberculous mycobacterial infections]. | 2001 | 11977501 | |
| cleavage and purification of intein fusion proteins using the streptococcus gordonii spex system. | a gram-positive bacterial expression vector using streptococcus gordonii has been developed for expression and secretion, or surface anchoring of heterologous proteins. this system, termed surface protein expression system or spex, has been used to express a variety of surface anchored and secreted proteins. in this study, the mycobacterium xenopi (mxe) gyra intein and chitin binding domain from bacillus circulans chitinase al were used in conjunction with spex to express a fusion protein to fac ... | 2001 | 11513092 |
| mycobacterium xenopi pulmonary infection in an hiv infected patient under highly active antiretroviral treatment. | highly active antiretroviral therapy (haart) is responsible for a striking reduction in aids related morbidity and mortality by partly restoring immune function. however, haart can also precipitate the development of clinically apparent opportunistic infections in patients with latent infections. we report a case of an hiv infected patient who developed granulomatous nodular and cavitatory lesions of the lungs due to mycobacterium xenopi as a manifestation of the immune restoration syndrome. | 2001 | 11713363 |
| identification of mycobacterium xenopi by gas chromatography. | for the purposes of the following study we cultured 32 strains of mycobacterium xenopi isolated from clinical specimens and several strains of other slowly growing mycobacteria. the cultures were grown in liquid medium and then analysed--after saponification, methylation, extraction with organic solvent and washing of the organic phase--using a highly sensitive manual gas-liquid chromatographic assay for the determination of secondary alcohol 2-oh-docosanol. the percentage of this compound was c ... | 2001 | 11209848 |
| [pneumonia in patients with chronic lymphocytic leukemia. study of 30 episodes]. | to analyse the etiology, diagnostic methods and response to therapy in 30 episodes of pneumonia diagnosed in 17 patients with chronic lymphocytic leukemia (cll) between 1995 and 2000. | 2001 | 11412696 |
| pulmonary resection for mycobacterium xenopi pulmonary infection. | results of medical therapy for mycobacterium xenopi pulmonary infection remain unreliable. pulmonary resection may be beneficial to patients whose disease is localized and who can tolerate a resectional operation. | 2001 | 11789763 |
| mycobacterium xenopi osteomyelitis in a patient on highly active antiretroviral therapy (haart). | skeletal infections with atypical mycobacteria are a manifestation of advanced hiv disease, most patients having cd4 counts of less than 100 cells/mm(3). we report a case of mycobacterium xenopi vertebral osteomyelitis in a patient on haart with a cd4 count of 490 cells/mm(3) and viral load below the level of detection at the time of diagnosis. | 2001 | 11368825 |
| first randomised trial of treatments for pulmonary disease caused by m avium intracellulare, m malmoense, and m xenopi in hiv negative patients: rifampicin, ethambutol and isoniazid versus rifampicin and ethambutol. | the treatment of pulmonary disease caused by opportunist mycobacteria is controversial. it is uncertain whether in vitro sensitivity testing predicts clinical response in the way it does for mycobacterium tuberculosis. the literature suggests that the combination of rifampicin (r) and ethambutol (e) is important whereas isoniazid (h) may not be, but to date there have been no published reports of randomised controlled trials in the treatment of these conditions. the british thoracic society has ... | 2001 | 11182006 |
| cutaneous infection with mycobacterium xenopi. | 2001 | 11204610 | |
| performance assessment of new multiplex probe assay for identification of mycobacteria. | a new dna probe assay (inno lipa mycobacteria; innogenetics, ghent, belgium) for the simultaneous identification, by means of reverse hybridization and line-probe technology, of mycobacterium tuberculosis complex, mycobacterium kansasii, mycobacterium xenopi, mycobacterium gordonae, the species of the mycobacterium avium complex (mac), mycobacterium scrofulaceum, and mycobacterium chelonae was evaluated on a panel of 238 strains including, besides representatives of all the taxa identifiable by ... | 2001 | 11230430 |
| [mycobacterium xenopi: epidemiological and bacteriological features]. | mycobacterium xenopi is a scotochromogenic slow-growing atypical mycobacteria, with a thermostable catalase, no production of niacin and whose cell wall contains types i and vi long-chain fatty acids. cosmopolitan, it is mainly recovered in tap-warm water. the contamination occurs through aerosol inhalation, water ingestion or use of contaminated medical or surgical equipment. m. xenopi is an opportunistic pathogen; the infection is facilitated by the incidental introduction of the bacteria in t ... | 2001 | 11282515 |
| lung cancer occurring with mycobacterium xenopi and aspergillus. | two cases which associate mycobacterium xenopi pulmonary infection, aspergilloma and preoperative unsuspected lung cancer are related. to our knowledge, the association of these three pathologies has never been previously reported. these two cases, suggest that infected chronic lung lesions, especially in smokers, should be closely monitored and be surgically removed in order to prevent further complications. | 2001 | 11423302 |
| efficacies of clarithromycin regimens against mycobacterium xenopi in mice. | mice were infected intravenously with 3.5 x 10(7) cfu of mycobacterium xenopi and treated with various clarithromycin-containing regimens or left untreated for 4 weeks. all nine of the clarithromycin-containing regimens reduced the cfu counts to the levels below the pretreatment values, indicating that these regimens had a bactericidal effect on m. xenopi in mice. the rifampin-isoniazid-ethambutol regimen was significantly less bactericidal than clarithromycin alone or clarithromycin-containing ... | 2001 | 11600387 |
| mycobacterium xenopi spinal infections after discovertebral surgery: investigation and screening of a large outbreak. | mycobacterium xenopi spinal infections were diagnosed in 1993 in patients who had undergone surgical microdiscectomy for disc hernia, by nucleotomy or microsurgery, in a private hospital. contaminated tap water, used for rinsing surgical devices after disinfection, was identified as the source of the outbreak. several cases were recorded in the 4 years after implementation of effective control measures because of the long time between discectomy and case detection. the national health authoritie ... | 2001 | 11551599 |
| [acquired immunodeficiency syndrome and pulmonary mycobacterium xenopi infection. role of computerized tomography]. | mycobacterium xenopi is one of the most common agents responsible for nontubercular mycobacterial pulmonary disease on aids patients. these lesions have been studied with conventional radiography, while ct has been used in patients with aspecific mycobacterioses or non-aids pulmonary conditions from mycobacterium xenopi. | 2000 | 11262821 |
| mycobacterium xenopi and related organisms isolated from stream waters in finland and description of mycobacterium botniense sp. nov. | three scotochromogenic mycobacterium xenopi-like organisms were isolated from stream waters in finland. these strains grew at 36-50 degrees c but not at 30 degrees c. one of the three strains was fully compatible with the m. xenopi type strain according to glc-ms, biochemical tests, and 16s rdna and 16s-23s rdna internal transcribed spacer (its) sequencing. two of the strains closely resembled m. xenopi in lipid analyses and biochemical tests, but analysis by glc-ms verified the presence of two ... | 2000 | 10826815 |
| [lung disease due to mycobacterium xenopi excluding aids. apropos of 8 cases]. | between 1994 and early 1999, mycobacterium xenopi was isolated in 11 hiv-negative patients seen at the respiratory disease department of the dijon university hospital. eight of these patients met the criteria of lung infection. clinical and radiological features simulated pulmonary tuberculosis which delayed diagnosis until the germ was identified. treatment is considered to be mandatory though it is difficult to manage and often disappointing. in spite of long-term medical care, sometimes assoc ... | 2000 | 10859767 |
| [mycobacterium xenopi pulmonary infection after starting high efficiency treatment with indinavir]. | 2000 | 10953599 | |
| [negative effect of the components of the lysis-centrifugation system in the growth of mycobacteria in mgit and septi-chek afb liquid media]. | the lysis-centrifugation system (isolator system) is a technique with excellent results in the recovery of mycobacteria from blood specimens. this system consists mainly of saponin (sap), polypropylenglycol (ppg), and sodium polianthol sulfonate (sps). the objective of this work was to determine the effect of sap, ppg, and sps on the growth of mycobacterium avium, m. kansasii, m. tuberculosis, and m. xenopi in fluid culture media mgit and septi-chek afb. | 2000 | 11149167 |
| differentiation of mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using peptide nucleic acid-fluorescence in situ hybridization probes. | a blinded comparison of peptide nucleic acid-fluorescence in situ hybridization (pna-fish) with routine identification methods was performed on 74 consecutively positive mycobacterial liquid cultures. all mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81. 5%) nontuberculous cultures were correctly identified (including one mixed culture). five isolates yielded no reaction with either probe and were identified as mycobacterium xenopi, mycobacterium fortuitum, or mycobacterium flaves ... | 2000 | 10618138 |
| mycobacterium heckeshornense sp. nov., a new pathogenic slowly growing mycobacterium sp. causing cavitary lung disease in an immunocompetent patient. | a pathogenic scotochromogenic mycobacterium xenopi-like organism was isolated from the lung of an immunocompetent young woman. this pathogen caused severe bilateral cavitary lung disease, making two surgical interventions necessary after years of chronic disease. this case prompted us to characterize this mycobacterium by a polyphasic taxonomic approach. the isolate contained chemotaxonomic markers which were typical for the genus mycobacterium, i.e., the meso isomer of 2,6-diaminopimelic acid, ... | 2000 | 11060075 |
| evaluation of the bactericidal efficiency of a 2% alkaline glutaraldehyde solution on mycobacterium xenopi. | mycobacterium xenopi (m. xenopi) has been implicated in hospital-acquired infections associated with colonization of hospital water systems. m. xenopi is considered to be as resistant as other atypical mycobacteria, which are known to be resistant to many disinfecting treatments. however, the efficacy of disinfectants on this organism has not yet been studied. therefore we decided to evaluate its susceptibility to 2% alkaline glutaraldehyde solution, which is commonly used in hospitals. tests we ... | 2000 | 11023727 |
| hiv-related nontuberculous mycobacterial infection: incidence, survival analysis and associated risk factors. | to evaluate the incidence and survival time for aids-patients affected by different stages of nontuberculous mycobacterial (ntm) infection we performed a retrospective study. data of 1540 hospitalised aids-patients was analyzed with respect to survival time and incidence rates. the overall incidence rate of ntm following aids was 16.6/100 person-years (py), with an increase from 12.1/100py (1987-1990) to 18.9/100py (1991-1994). antiretroviral therapy (art) and toxoplasmosis prophylaxis reduced t ... | 2000 | 11076783 |
| increasing incidence of mycobacterium xenopi at bellevue hospital: an emerging pathogen or a product of improved laboratory methods? | to investigate the dramatic rise in number of mycobacterium xenopi isolates identified in our mycobacteriology laboratory, and to determine if this increase was due to emerging clinical pathology or to changes in culture technique. | 2000 | 11083687 |
| comparison of the automated nonradiometric bactec mgit 960 system with löwenstein-jensen, coletsos, and middlebrook 7h11 solid media for recovery of mycobacteria. | the aim of this study was to evaluate the sensitivity of as well as the time to detection of mycobacteria by three procedures: solid media with traditional reading, microscopy on solid media, and liquid culture using the automated nonradiometric bactec mgit 960 system. a total of 2832 respiratory specimens were tested, 315 of which were positive for mycobacteria. the most frequently isolated species was mycobacterium tuberculosis (201 isolates). one hundred twenty mycobacteria other than tubercu ... | 2000 | 10898135 |
| protein splicing and related forms of protein autoprocessing. | protein splicing is a form of posttranslational processing that consists of the excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by the concomitant joining of the flanking polypeptide sequences, the exteins, by a peptide bond. it requires neither cofactors nor auxiliary enzymes and involves a series of four intramolecular reactions, the first three of which occur at a single catalytic center of the intein. protein splicing can be modulated by mutation and ... | 2000 | 10966466 |
| [hemophagocytic syndrome associated with cytomegalovirus and mycobacterium xenopi disseminated disease in a patient infected by the human immunodeficiency virus who had a fatal outcome]. | 2000 | 10721587 | |
| mycobacterium xenopi infection of the spine: a case report and literature review. | a case report of mycobacterium xenopi. | 2000 | 10749641 |
| [mycobacterium xenopi osteoarthritis of the ankle in a patient followed for psoriatic rheumatism]. | mycobacterium xenopi is an uncommon cause of osteoarticular sepsis. however, a recent series of m. xenopi spondylodiscitis emphasizes the potential risk after invasive procedures. | 2000 | 10761518 |
| mycobacteria other than tuberculosis with an emphasis on mycobacterium xenopi in clinical specimens from aids patients at the university hospital of vienna from 1989 to 1996. | this study was carried out to provide an overview of the frequency of various mycobacterial species isolated from aids patients at the university hospital of vienna from 1989 to 1996. mycobacterium xenopi was found to be the second most common nontuberculous mycobacterial species (92 specimens from 30 patients) and was cultured predominantly from respiratory tract specimens. in 55% of patients, chest x-rays taken at the time of isolation demonstrated pathologic changes which could not be attribu ... | 1999 | 10081122 |
| mycobacterial infections in lung transplant recipients. | immunosuppression and chronic lung disease are known risk factors for mycobacterial infection and might be expected to develop with an increased frequency in lung transplant recipients. we therefore sought to document the incidence and type of mycobacterial infections in a large lung transplant program. | 1999 | 10084486 |
| the cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins. | mini-inteins derived from synechocystis sp. (ssp dnab intein) and mycobacterium xenopi (mxe gyra intein) that have been modified to cleave peptide bonds at their c and n termini, respectively, were cloned in-frame to the n and c termini of a target protein. peptide bond cleavage of the modified inteins generated an n-terminal cysteine and a c-terminal thioester on the same protein. these complementary reactive groups underwent intra- or intermolecular condensation to generate circular or polymer ... | 1999 | 10373440 |
| purification of proteins fused to either the amino or carboxy terminus of the mycobacterium xenopi gyrase a intein. | the mycobacterium xenopi gyrase a mini-intein has been engineered to yield a controllable n-terminal or c-terminal, single-splice-junction autocleavage element. when combined with an affinity tag, these modified mini-inteins can be used to purify target proteins after a single combined chromatography/cleavage step. cleavage at the intein n terminus was induced with thiol reagents, while cleavage at the intein c terminus was induced by a temperature shift to 16 degrees-25 degrees c. different pre ... | 1999 | 10407673 |
| successful treatment of pulmonary mycobacterium xenopi infection in a natural killer cell-deficient patient with clarithromycin, rifabutin, and sparfloxacin. | isolation of mycobacterium xenopi from the respiratory tract may indicate pneumonia, often clinically indistinguishable from tuberculosis. resistance to the classic antituberculous drugs renders the treatment of these infections problematic. we report on a case of cavernous pneumonia caused by m. xenopi in a 36-year-old male with natural killer cell deficiency but without severe immunodeficiency. he was successfully treated with a novel triple-drug combination comprising clarithromycin, sparflox ... | 1999 | 10433574 |
| overexpression of fas/cd95 and fas-induced apoptosis in a patient with idiopathic cd4+ t lymphocytopenia. | the mechanisms of apoptosis have become better understood, in part with the discovery of fas/cd95. we report the case of a patient characterized by a decreased cd4+ t cell count and an overexpression of fas/cd95 resulting in apoptosis. a 54-year-old man presented with disseminated mycobacterium xenopi infection. analysis showed cd4+ t lymphopenia. tests for human immunodeficiency virus (hiv) types 1 and 2 were negative. we compared the patient with eight healthy controls and five hiv-infected pa ... | 1999 | 10452627 |
| non-tuberculous mycobacterial tenosynovitis: a review. | the clinical characteristics, outcome and treatment of non-tuberculous mycobacterial tenosynovitis are reviewed. from lesions localized in the hand, 10 different species of non-tuberculous mycobacteria have been reported. the most common are mycobacterium marinum and mycobacterium kansasii. other less frequent organisms are mycobacterium avium complex, mycobacterium szulgai, mycobacterium terrae, mycobacterium fortuitum, mycobacterium chelonae, mycobacterium abscessus, mycobacterium malmoense an ... | 1999 | 10482048 |
| mycobacterium xenopi infection of a 50-year-old oil plombage complicated by bronchopleural and pleurocutaneous fistulas. | we report the rare combination of simultaneous bronchopleural and pleurocutaneous fistulas 50 years after oil plombage, together with infection of both the plombage and the contralateral lung with mycobacterium xenopi. our case documents imaging patterns of complex fistula formation and subsequent infection resulting from oil plombage. our case also emphasizes the infectious potential of mycobacterium xenopi. | 1999 | 10524814 |
| simple and rapid identification of different species of mycobacteria by pcr. | a simple polymerase chain reaction (pcr) assay for rapid identification of different species of mycobacteria was developed. this pcr is based on the use of conserved sequences to amplify the genome of several mycobacterial species. the amplification patterns obtained were specific and reproducible for the species tested. in particular, we could identify mycobacterium tuberculosis and mycobacterium bovis (both produced the same pattern), mycobacterium avium, mycobacterium kansasii, mycobacterium ... | 1999 | 10208805 |
| systemic mycobacterioses in aids patients as determined by blood cultures on biphasic medium. | bacteremia due to mycobacteria can occur in aids patients in whom a rapid diagnosis is extremely important in order to plan a therapeutic conduct. blood culture of mycobacteria using a biphasic system was set up in the regional laboratories of the adolfo lutz institute, sp (campinas, ribeirão preto, santo andré, santos, são josé do rio preto and sorocaba). during a three year period (1994-97), 1521 blood samples were analyzed from 1336 aids patients, with cd4+ cell count < 100/ml, hematocrit < 3 ... | 1999 | 10425659 |
| use of bactec mgit 960 for recovery of mycobacteria from clinical specimens: multicenter study. | the bactec mgit 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. its performance was compared to those of the radiometric bactec 460 instrument and egg-based lowenstein-jensen medium. an identical volume of sample was inoculated in different media, and incubation was carried out for 6 weeks with the automatic systems and for 8 weeks on solid media. a total of 2,567 specimens obtained from 1,631 patients wer ... | 1999 | 10523555 |
| [clinical significance of the isolation of mycobacterium xenopi]. | 1999 | 10422079 | |
| immunopathology as a result of highly active antiretroviral therapy in hiv-1-infected patients. | unusual clinical inflammatory syndromes associated with underlying previously unrecognized opportunistic infections are increasingly being noted shortly after starting highly active antiretroviral therapy (haart). this study examined the possible relationship between such unexpected disease manifestations and in vitro parameters of microbial antigen-specific immune reactivity in patients infected with hiv-1 who had a mycobacterium avium intracellulare or mycobacterium xenopi infection. | 1999 | 10202823 |
| mycobacterium xenopi lung infection. | 1999 | 10678220 | |
| [changes in the kidneys in patients with successive findings of mycobacterium xenopi and mycobacterium fortuitum in the urine--report of 16 cases]]. | environmental or mott (mycobacteria other than tubercle bacilli) mycobacteria are found in both living environment and most of the food we consume. these mycobacteria can induce a disease in humans, although they rarely do. there are a few reports of urogenital infections caused by these bacteria. this is a report of 16 patients with successive findings of mycobacterium xenopi and mycobacterium fortuitum in the urine. | 1999 | 10624381 |
| septic arthritis in the knee due to mycobacterium xenopi in a patient undergoing hemodialysis. | 1999 | 10524998 | |
| clinical and radiographic manifestations of uncommon pulmonary nontuberculous mycobacterial disease in aids patients. | to determine the clinical and radiographic findings of nontuberculous mycobacteria (ntm) other than mycobacterium avium complex (mac) and mycobacterium kansasii in aids compared with non-aids patients. | 1998 | 9674460 |
| no news is good news: opportunistic infections. | the 38th interscience conference on antimicrobial agents and chemotherapy (icaac) was notable for not having any oral sessions on opportunistic infections. this is an indication of the enormous success of combination antiretroviral therapy, which has greatly reduced the occurrences of opportunistic infections. hepatitis c was discussed at the conference, but it was treated as a co-morbid condition rather than an opportunistic infection. two studies addressed the question of stopping pneumocystis ... | 1998 | 11366129 |
| lymphadenitis and peritonitis caused by mycobacterium xenopi in a cat. | 1998 | 12075548 | |
| [mycobacterium xenopi, a newcomer?]. | 1998 | 11767362 | |
| mycobacterium xenopi pulmonary infection: evaluation with ct. | our goal was to describe the ct appearances of mycobacterium xenopi pulmonary infection. | 1998 | 9530384 |
| mycobacterial linear plasmids have an invertron-like structure related to other linear replicons in actinomycetes. | the authors previously identified large plasmids in mycobacterium xenopi, m. branderi and m. celatum which appeared to have a linear topology. this study has confirmed the presence of such linear plasmids in mycobacteria, including m. avium, and demonstrated that the ends of these replicons are covalently bound with protein(s), suggesting an invertron-like structure. the termini of one 25 kb plasmid, designated pclp, from m. celatum were cloned and the first 500 bp of each terminus were sequence ... | 1998 | 9695931 |
| primary psoas muscle abscess due to mycobacterium xenopi. | 1998 | 9455560 | |
| radiographic findings in patients with acquired immunodeficiency syndrome, pulmonary infection, and microbiologic evidence of mycobacterium xenopi. | the authors studied radiographs and clinical histories of 29 patients with acquired immunodeficiency syndrome, symptoms of pulmonary infection, and simultaneous microbiologic evidence of mycobacterium xenopi in the respiratory tract. the presence, nature, and distribution of radiographic abnormalities were determined and analyzed in accord with clinical information. in 26 (90%) patients, m. xenopi was the only microorganism that could be isolated. chest radiographs were normal in 13 patients (45 ... | 1998 | 9799136 |
| infections due to nontuberculous mycobacteria in children with leukemia. | we reviewed the spectrum of infections due to nontuberculous mycobacteria (ntm) in children with leukemia. three children acquired such infections. one patient developed pneumonia after the cessation of chemotherapy when mycobacterium xenopi was identified in his lung biopsy specimen. he required 2 years of treatment with antituberculous agents and clarithromycin. cultures of central and peripheral blood specimens from two patients yielded mycobacterium fortuitum and mycobacterium chelonae, resp ... | 1998 | 9827274 |
| inteins in mycobacterial gyra are a taxonomic character. | the a subunit of dna gyrase in mycobacteria is frequently subjected to splicing events as its gene, gyra, harbours an insertion encoding an intein. investigation of a number of different isolates of mycobacterium kansasii, mycobacterium malmoense, mycobacterium marinum, mycobacterium ulcerans and mycobacterium xenopi demonstrated that the presence of gyra inteins is not random but a taxonomic character specific for a given taxon at a species or subspecies level. | 1998 | 9493395 |