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presumptive identification of mycobacterium xenopi with the radiometric bactec system. 19911748129
effect of anorexia nervosa on pulmonary immunocompetence.pulmonary infection due to opportunistic organisms and usually nonpathogenic mycobacteria may occur whenever immune defenses are weakened by external factors. these factors include neutropenia, treatment with corticosteroids or cytotoxic agents, previous antibiotic therapy, and nosocomial hazards (eg, catheters, ventilators). nutritional deprivation is also often implicated as an important coexistent variable. an infection due to mycobacterium xenopi (usually a nonpathogenic mycobacterium) in a ...19911925716
activities of pefloxacin and ofloxacin against mycobacteria: in vitro and mouse experiments.the minimal inhibitory concentrations for 90% of strains (mic90) of ofloxacin against mycobacterium tuberculosis and mycobacterium xenopi was 2 mg/l. this was three dilutions lower than that of pefloxacin and was well within the range of drug concentrations achievable in man. the antituberculosis activities of both quinolones were independent of resistance of the strains to other antimycobacterial agents. mycobacterium avium-intracellulare was resistant to both compounds with mic90s greater than ...19911909062
a new type of serine-containing glycopeptidolipid from mycobacterium xenopi.an unknown immunogenic glycopeptidolipid, named gpl x-1, was isolated from mycobacterium xenopi, which is a nontuberculous mycobacterium responsible for pulmonary and disseminated infectious diseases mainly occurring in immunocompromised patients. the glycopeptidolipid was purified until homogeneity, in the native form, by direct phase high performance liquid chromatography. a new route is proposed for the structural elucidation of its unusual lipopeptidic core. the presence of allothreonine (at ...19911902834
[lung changes caused by mycobacterium xenopi infection in a patient with bone marrow transplantation: problems in differential diagnosis].pulmonary affections caused by atypical mycobacteria are an increasingly common problem particularly in patients with immune deficiency disorders. we here report a case of pulmonary infiltrates due to mycobacterium xenopi in a patient after allogeneic bone marrow transplantation for acute myeloid leukemia in first complete remission and under immunosuppressive treatment with prednisolone and cyclosporin a. while sputum cultures, serology as well as bronchial lavage and transbronchial biopsy rema ...19911857690
mycobacterium xenopi isolation from clinical specimens in the florence area: review of 46 cases.the occurrence of mycobacterium xenopi (mx) isolates is not homogeneous in various geographic zones. in the florence area, between 1975-1989, strains of mx from 64 different patients have been isolated. the review of bacteriological and clinical data of 46 of them, from whose sputum mx had been grown, allowed to diagnose for 26% the commensal nature of this finding, for 41% the concomitance with a tubercular infection and for the remaining 33% the pathogenicity of this microorganism. the increas ...19911783061
disease association of antibodies to human and mycobacterial hsp70 and hsp60 stress proteins.structural homology between microbial and human stress proteins has been postulated to be a basis for autoimmunization in chronic inflammatory diseases. therefore, we estimated by elisa titration the antibody levels to mycobacterial (m) and human (h) recombinant hsp70 and m-hsp65 heat-shock proteins in sera of patients with crohn's disease (n = 29), ulcerative colitis (n = 20) and nontuberculous mycobacterial disease of the lungs (n = 20). antibodies to h-hsp60, separated by two-dimensional gel ...19921638774
fulminant hepatic failure complicating the treatment of mycobacterium xenopi. 19921630355
mycobacterium xenopi, mycobacterium fortuitum, mycobacterium kansasii, and other nontuberculous mycobacteria in an area of endemicity for aids.between 1981 and 1990, cultures of specimens from 86 patients at state university of new york-health sciences center at brooklyn were positive for nontuberculous mycobacteria other than mycobacterium avium/mycobacterium intracellulare complex or mycobacterium gordonae. the most common species isolated were mycobacterium xenopi (33), mycobacterium fortuitum (28), mycobacterium kansasii (7), and mycobacterium chelonae (6). thirty-five patients (41%) had clinical and/or serological evidence of huma ...19921617056
rapid differentiation of mycobacterium xenopi from mycobacteria of the mycobacterium avium-intracellulare complex by pyrolysis mass spectrometry.thirty four cultures of slow growing, tween-80 negative mycobacteria were analysed by pyrolysis mass spectrometry. the results showed that pyrolysis mass spectrometry could positively distinguish strains of mycobacterium xenopi from those of the mycobacterium avium-intracellulare (mai) complex. pyrolysis mass spectrometry may be a useful technique for the rapid characterisation of non-tuberculous mycobacteria in such clinical settings as their isolation from immunocompromised patients-for exampl ...19921577977
secondary fatty alcohols of mycobacterium xenopi.secondary alcohols of mycobacterium xenopi were studied by gas chromatography and gas chromatography-mass spectrometry. mycobacterial cells were hydrolysed and the liberated alcohols separated by extraction and analysed both underivatized and as trimethylsilyl, methyl ether- and pentafluorobenzoyl derivatives. seven straight-chain secondary alcohols containing from 18 to 24 carbon atoms and two branched-chain secondary alcohols with 21 and 23 carbon atoms were present in all of the studied strai ...19921487721
mycobacterium xenopi, a potential human pathogen.mycobacterium xenopi is infrequently recognized as a cause of pulmonary disease. during a 12-year survey (1978-89),. 108 strains of this mycobacterium were isolated from 90 persons and 6 hot water samples. from 87 patients 89 occasional strains of m. xenopi were isolated, and 3 patients were diagnosed as having pulmonary mycobacteriosis caused by it. the treatment and the response in these three cases were variable, depending on clinical conditions and sensitivity to drugs. most of the strains i ...19921468888
identification of mycobacterium avium complex strains and some similar species by high-performance liquid chromatography.strains of mycobacterium avium, mycobacterium intracellulare, mycobacterium scrofulaceum, mycobacterium xenopi, and mycobacterium gordonae were identified by high-performance liquid chromatography (hplc) analysis of mycolic acids as bromophenacyl esters. hplc criteria were used to develop a flow chart identification scheme, which was evaluated in our laboratory with a set of 234 strains representing five species and a hitherto undescribed species. correct identifications of m. gordonae and m. xe ...19921400970
[a lupus-vulgaris like atypical mycobacteriosis caused by mycobacterium xenopi (lupus xenopi)].a case of lupus-vulgaris-like infection caused by mycobacterium xenopi in a 62-year-old immunocompetent female patient is presented. a large cutaneous infiltration was seen in the right periorbital region. histological examination revealed a granulomatous reaction of epithelioid cells and giant cells. m. xenopi was isolated from biopsy material and tuberculosis could be excluded. isoniazid was effective in healing the lesion within a year. such infections are well known for other mycobacteria bu ...19921399603
[extracellular and intracellular activity of sparfloxacin against mycobacterium avium complex and mycobacterium xenopi].activity of the new fluoroquinolone sparfloxacin against 30 strains of m. avium complex and 25 strains of m. xenopi was tested in vitro. sparfloxacin was used alone (determination of mics and mbcs) and in combination with ethambutol and rifabutin. synergy studies with determination of the fic and fbc indices showed that the sparfloxacin-ethambutol combination was synergistic against 10 m. avium complex strains and 12 m. xenopi strains. with the three-drug combination (sparfloxacin-ethambutol-rif ...19921323093
use of 1h nmr roesy for structural determination of o-glycosylated amino acids from a serine-containing glycopeptidolipid antigen.a serine-containing glycopeptidolipid antigen isolated from mycobacterium xenopi typified a new class of mycobacterial glycopeptidolipid antigens devoid of the c-mycoside core structure [rivière, m., & puzo, g. (1991) j. biol. chem. 266, 9057-9063]. the lipopeptide core assigned to c12-ser-ser-phe-allothr-och3 exhibits three potential sites of glycosylation. the carbohydrate parts are composed of 3-o-methyl-6-deoxy-alpha-l-talopyranosyl and 2,3,4-tri-o-methyl-l- rhamnopyranosyl(alpha 1----3)-2-o ...19921314643
spinal infection by mycobacterium xenopi in a non-immunosuppressed patient.a previously fit 77-year-old woman was found to have a paravertebral abscess. this was aspirated, yielding mycobacterium xenopi, but no other pathogens. antituberculosis chemotherapy with four first-line agents was commenced, with some response but continuing pain. reports of extrapulmonary m. xenopi infections are rare. most previous cases have involved immunosuppressed patients. this is the first reported case of spinal m. xenopi infection in a non-immunosuppressed patient, but radiological ev ...19921292722
[ofloxacin in the treatment of pulmonary changes caused by mycobacterium avium and mycobacterium xenopii].two cases of atypical mycobacterial infections are reported. the first caused by mycobacterium avium in a patient with silicosis, the second by mycobacterium xenopii in a subject with evidence of extensive post-tuberculosis pulmonary lesions. both were treated with ofloxacin with good results.19921290992
application of gas chromatography-mass spectrometry for rapid detection of mycobacterium xenopi in drinking water.gas chromatography-mass spectrometry (gc-ms) was used to detect 2-docosanol, a secondary alcohol characteristic of mycobacterium xenopi, in 7 of 10 analyzed drinking water samples culture positive for that species. gc-ms was also used to detect tuberculostearic acid. both of these chemical markers were analyzed as halogenated derivatives in the negative-ion-chemical-ionization mode. the numbers of cfu of m. xenopi were lowest in the three gc-ms-negative samples. the described gc-ms method is use ...19921482179
comparative in-vitro activities of the new quinolone, bay y 3118, and ciprofloxacin, sparfloxacin, tosufloxacin, ci-960 and ci-990.the in-vitro activity of the new quinolone, bay y 3118, was compared with that of ciprofloxacin, tosufloxacin, sparfloxacin, ci-960 and ci-990 against 1640 isolates belonging to 117 bacterial species. against members of the enterobacteriaceae, bay y 3118 was as active as ci-960 and ci-990, up to four-fold more active than ciprofloxacin and tosufloxacin and up to 16-fold more active than sparfloxacin. the majority of enterobacteriaceae which were resistant to ciprofloxacin (mics > or = 2 mg/l) we ...19937605398
further structural definition of a new family of glycopeptidolipids from mycobacterium xenopi.the highly antigenic surface glycolipids of serovars of the mycobacterium avium complex are glycopeptidolipids of the general structure [formula: see text] however, it has recently been shown [rivière, m., & puzo, g. (1991) j. biol. chem. 266, 9057-9063; rivière, m., & puzo, g. (1992) biochemistry 31, 3575-3580] that the characteristic glycopeptidolipid of a strain of mycobacterium xenopi is of the structure [formula: see text] [monosaccharide, 3-o-ch3-6-deoxy-alpha-l-talopyranose; tetrasacchari ...19937678195
dna fingerprinting of mycobacterium bovis strains by restriction fragment analysis and hybridization with insertion elements is1081 and is6110.strains of mycobacterium bovis, the causative organism of bovine tuberculosis, can be clearly distinguished from each other by restriction fragment analysis. this method of dna fingerprinting has been used for many epidemiological studies in new zealand, but the technique presents practical difficulties that hinder its widespread use. the insertion element is6110 is being widely used as a dna probe for distinguishing restriction fragment polymorphisms among strains of mycobacterium tuberculosis. ...19938099083
mycobacterium celatum sp. nov.a new slowly growing nonphotochromogenic mycobacterium species of clinical importance is described. the biochemical characteristics of this organism were similar to those of mycobacterium xenopi and members of the mycobacterium avium complex. however, none of the strains reacted with commercially available genetic probes for the m. avium complex. the strains were resistant to most antituberculosis drugs. multilocus enzyme electrophoresis revealed two original electrophoretic types, which was sug ...19938102246
a nosocomial pseudo-outbreak of mycobacterium xenopi due to a contaminated potable water supply: lessons in prevention.to determine risk factors for mycobacterium xenopi isolation in patients following a pseudo-outbreak of infection with the organism.19938132983
in-vitro activities of quinolones against mycobacteria.from the results of recently-published in-vitro studies, we have reviewed the activities of novel quinolones which are currently available, either commercially or for investigative purposes, against mycobacterium tuberculosis, the atypical mycobacteria (principally the mycobacterium avium complex, mycobacterium chelonae, mycobacterium xenopi, mycobacterium marinum and mycobacterium fortuitum) and mycobacterium leprae. we have also evaluated the effects of the various methods for determining the ...19938144420
[pneumonia due to a rare atypical mycobacterium in aids].a 38-year-old man, hiv-positive for 6 years, developed fever and cough with deterioration in his general state. chest radiography demonstrated an infiltration in the left upper lobe and computed tomography showed a septated cavity. three bronchioalveolar lavages over 4 weeks recovered klebsiella, candida, pseudomonas and staphylococcus in the lavage fluid. acid-fast rods were not found in any of the microscopic preparations. his clinical condition and the radiological findings deteriorated despi ...19938223223
post-operative acute respiratory failure associated with mycobacterium xenopi. 19938227731
[pulmonary disease due to mycobacterium xenopi].a 54-year old man with pulmonary disease due to mycobacterium xenopi is described. he had a history of pulmonary tuberculosis at 8 years of age. he was admitted to our hospital in october 1992, complaining of productive cough and fever. a chest x-ray on october 20 showed an infiltrative shadow with a cavity, although chest x-ray picture on october 2 showed only inactive shadow, in the right upper lobe. acid-fast organisms were seen in his sputum specimens and m. xenopi was identified by culture. ...19938255073
water-borne household infections due to mycobacterium xenopi.of 21 m. xenopi excretors recorded in prague in 1990, 13 suffered from a serious pulmonary disease and the organisms were detected repeatedly in all of them. in 11 flats of these excretors water samples were collected from faucets and showers and m. xenopi was detected in five of them, as well as in five neighbouring flats. in flats of six remaining excretors and 12 adjoining flats, m. xenopi was not found. however, in 14 of 28 examined flats, the clinically insignificant m. gordonae was isolate ...19938004044
structure of a novel glycopeptidolipid antigen containing a o-methylated serine isolated from mycobacterium xenopi. complete 1h-nmr and 13c-nmr assignment.gpl x-1, a novel glycopeptidolipid (gpl) isolated from mycobacterium xenopi (cipt 140 35004), has recently been found to typify a new class of mycobacterial glycopeptidolipids devoid of c-mycoside core structure, the so-called serine-containing glycopeptidolipid [rivière, m. & puzo, g. (1991) j. biol. chem. 266, 9057-9063]. here we report the purification and characterization of a novel serine-containing gpl termed gpl x-iib, isolated from the m. xenopi strain nctc 10042. on thin-layer chromatog ...19938513789
bronchoscopy-associated mycobacterium xenopi pseudoinfections.mycobacterium xenopi typically accounts for less than 0.3% of all clinical mycobacterial isolates. over a 37-mo period, 21 (35%) of 60 mycobacterial isolates from a michigan hospital were identified as m. xenopi. hospital, laboratory, and bronchoscopy records were reviewed to determine case characteristics, develop a case series, and calculate procedure-specific m. xenopi isolation rates. a case-control study was conducted to elucidate aspects of the bronchoscopy procedure associated with m. xen ...19948025757
[radiometric determination of mycobacterium avium-intracellulare and mycobacterium xenopi sensitivity to antitubercular agents].using the macrodilution radiometric technique in a bactec 460 apparatus, the authors assessed in 25 strains of the m. avium complex and 20 strains of m. xenopi the mic of the following chemotherapeutic agents: ciprofloxacine, clofazimine, rifampin, cycloserine, kanamycin, etionamide, ethambutol and amikacin. in all instances wild strains isolated in the czech republic in 1991-1992 were involved. in strains of the m. avium complex in the majority of drugs a major predominance of slightly or compl ...19947953081
pulmonary infection due to mycobacterium xenopi.we describe two cases of pulmonary infection due to mycobacterium xenopi (m. xenopi). both cases were men, ages 61 and 54 yr. in the first patient, lung infection due to m. xenopi occurred after gastrectomy. the second patient had an inactive m. tuberculosis infection. both had pulmonary symptoms including cough, sputum and fever. each chest x-ray showed an infiltrative shadow with a cavity in a unilateral, upper lobe. isolates from both patients were studied not only by microbiological characte ...19948000103
water as a possible factor of transmission in mycobacterial infections.mycobacterium kansasii and mycobacterium xenopi are the most frequent species occurring in water in the czech republic. in the endemic area of m. kansasii in heavy industry and mining areas of north moravia various mycobacterial species were detected in more than 20% of different water samples and m. kansasii was found in 1.5 to 1.9% of them, frequently in pit bathrooms and in the drinking water as well. mycobacterium xenopi was detected in 35 and 50% of water samples collected in households of ...19947697016
pulmonary infections caused by less frequently encountered slow-growing environmental mycobacteria.pulmonary mycobacteriosis is usually caused by mycobacterium tuberculosis or mycobacterium avium complex. there are, however, other slow-growing mycobacteria that can cause pulmonary infection. mycobacterium kansasii, mycobacterium malmoense, mycobacterium xenopi, mycobacterium szulgai and mycobacterium simiae typically infect middle-aged to elderly persons with preexisting lung disease. differentiation of infection with these five mycobacteria from infection with mycobacterium tuberculosis, by ...19947698120
[utility of the direct culture examination (ziehl-neelsen) using the bactec system for the presumptive identification of mycobacterium tuberculosis complex, mycobacterium avium complex, mycobacterium xenopi, and mycobacterium kansasii].the usefulness of the microscopic examination of bactec 12b and 13a growth medium as a method for the possible identification of m. tuberculosis complex, m. avium complex, m. xenopi, and m. kansasii was performed out to guide the selection of different genetic identification probes and, in the case of m. xenopi, the selection of the temperature of subcultures incubation.19947811323
pott's disease caused by mycobacterium xenopi: case report and review.we report a case of mycobacterium xenopi infection of the spine in a 70-year-old woman. the findings of our case and of five other published reports of bone or joint infection with m. xenopi illustrate the problems with diagnosis and management of infection with this organism. detection of m. xenopi in clinical specimens may require prolonged incubation at 37 degrees c or incubation at higher temperatures. furthermore, m. xenopi may be misidentified as mycobacterium avium-mycobacterium intracell ...19947888528
single and double drug susceptibility testing of mycobacterium avium complex and mycobacteria other than the tubercle (mott) bacilli by a micro-dilution broth minimum inhibitory concentration (mic) method.the incidence of mycobacterioses is increasing annually, especially in patients with aids. there is no clear correlation between in vitro drug susceptibility testing of mycobacteria other than the tubercle (mott) bacilli and the in vivo response. although in vitro, mott bacilli appear resistant, some patients respond to treatment possibly as a result of a synergistic action between the drugs being used.19947949075
[renal mycobacteriosis].there is a number of specific infections due to opportunistic mycobacteria which normally exist as environmental saprophytes. among them, renal mycobacteriosis is a disease similar to renal tuberculosis. the obvious causative agents are some mycobacteria: mycobacterium chelonei, mycobacterium fortuitum, mycobacterium xenopi, mycobacterium kansasii, mycobacterium avium complex, and mycobacterium simiae. it is not easy to prove that a renal disease is of mycobacterial origin. we suggest some crite ...199417972827
mycobacterium xenopi causing renal tuberculosis: a case report. 19948613158
laboratory and clinical evaluation of mycobacterium xenopi isolates.mycobacterium xenopi and mycobacterium avium complex (mac) are biochemically similar. to define the laboratory characteristics of m. xenopi that distinguish it from mac, 53 m. xenopi isolates from different areas in the united states and 47 isolates recovered at one hospital were evaluated by 13 biochemical tests, accuprobe mac (gen-probe, inc., san diego, ca, usa), colony morphology, formation of x-colonies, pigmentation in response to light, growth on macconkey agar without crystal violet, and ...19957554801
development of a species-specific probe for mycobacterium xenopi.in europe, mycobacterium xenopi is a frequently isolated species among opportunist mycobacteria, and represents one of the main agents of pulmonary infection due to non-tuberculous mycobacteria. conventional identification of mycobacteria is a time-consuming and laborious process. in this study, we propose a rapid and simple method for the identification of m. xenopi using polymerase chain reaction. the amplified product consists of a specific probe, present in all 38 m. xenopi strains tested, w ...19957569318
campath-1h, a humanized monoclonal antibody, in refractory rheumatoid arthritis. an intravenous dose-escalation study.to evaluate the biologic response, tolerability, and potential clinical effect of a humanized antilymphocyte monoclonal antibody, campath-1h, in patients with rheumatoid arthritis (ra).19957488279
structural elucidation of novel methylglucose-containing polysaccharides from mycobacterium xenopi.the structures of methylglucose-containing polysaccharides (meglc ps) from mycobacterium xenopi were investigated using high-ph anion-exchange chromatography and liquid secondary-ion mass spectrometry. we report the structure of two novel meglc ps, referred to as a and b. meglc ps a is composed of 16 d-glucopyranose residues, 11 of which are methylated, and meglc ps b contains 15 d-glucopyranose residues, 10 of which are methylated. the main structural feature of both meglc ps a and b, compared ...19957588770
mycobacterium xenopi infection masquerading as pulmonary tuberculosis in two patients infected with the human immunodeficiency virus.mycobacterium xenopi infections have rarely been reported among patients infected with the human immunodeficiency virus (hiv). we recently treated two hiv-infected men, neither of whom had a history of pulmonary disease or aids-defining conditions, and who had m. xenopi lung infections. both patients presented with night sweats, cough, and pleuritic chest pain. chest radiographs showed an upper-lobe nodule in the first patient and a perihilar cavitary infiltrate in the second patient. both patie ...19957620033
radiometric susceptibility testing of mycobacterium xenopi.mycobacterium xenopi is an opportunistic pathogen, frequently isolated in various areas of europe from pulmonary specimens, which may also cause infections in aids patients. we used the bactec radiometric system (becton dickinson, usa) with a procedure expressly adapted to the particular growth characteristics of m. xenopi to determine the susceptibility patterns of 40 clinical isolates to six antimicrobial drugs. the majority of the strains were resistant to ethambutol and susceptible to amikac ...19957666116
pott's disease caused by mycobacterium xenopi. 19958589186
mycobacterium branderi sp. nov., a new potential human pathogen.a number of mycobacterial strains with similar growth characteristics, metabolic properties, and lipid compositions, which were previously placed in the helsinki group (e. brander, e. jantzen, r. huttunen, a. juntunen, and m.-l. katila, j. clin. microbiol. 30:1972-1975, 1992), were characterized by performing 16s rrna gene sequencing. of the 14 strains studied, 9 had a unique, previously undescribed sequence in the variable region of 16s rrna. these nine strains, all of which were isolated from ...19958590682
rapid identification of mycobacterium xenopi from bacterial colonies or "bactec" culture by the polymerase chain reaction and a luminescent sandwich hybridization assay.oligonucleotide primers were used in the polymerase chain reaction (pcr) to amplify a specific 584-bp dna fragment, located in the 16s rna gene of mycobacterium xenopi. this set of primers, x222 and x224, was able to discriminate between the pathogen and other mycobacterial species as well as non-mycobacterial strains; it detected down to 3 fg of m. xenopi dna, i.e. about one genome equivalent. these oligonucleotide primers proved suitable for the routine identification of m. xenopi cultures, st ...19957569329
isolation of the newly described species mycobacterium celatum from aids patients.mycobacterium celatum is a recently described species which, on the basis of conventional tests, may be misidentified as mycobacterium xenopi or as belonging to the mycobacterium avium complex. only genomic sequencing or high-performance liquid chromatography of cell wall mycolic acids can presently allow a correct identification of this mycobacterium. two cases of infection due to m. celatum, in aids patients, are described here. the quantitative susceptibility pattern of the isolates to a wide ...19957699029
[pulmonary mycobacteriosis due to mycobacterium xenopi" in-vitro sensitivity to classical antitubercular agents and clinical development].out of 11 patients suffering from mycobacterium xenopi lung disease, 9 were treated with an empiric antituberculous triple chemotherapy until specific identification and antibiogram were available. despite the important "in vitro" resistance to drugs, most of the patients improved; in the other patients, the impairment was always due to the underlying pathology. we conclude that the "in vivo" response of m. xenopi infections to antituberculous drugs is little influenced by the "in vitro" sensiti ...19968669159
rifabutin versus placebo in combination with three drugs in the treatment of nontuberculous mycobacterial infection in patients with aids.the aim of this double-blind, randomized, placebo-controlled, 12-week study was to assess the efficacy of rifabutin (450 or 600 mg/d) in the treatment of disseminated nontuberculous mycobacterial infection in patients with aids. companion drugs in both arms of the study were ethambutol, clofazimine, and isoniazid. because of low accrual, the study was prematurely terminated when a total of 382 patients had been enrolled, of which 200 were eligible (i.e., their specimens were culture-positive for ...19968729209
iatrogenically induced spondylodiskitis due to mycobacterium xenopi in an immunocompetent patient. 19968729222
mycobacterium xenopi is1395, a novel insertion sequence expanding the is256 family.an insertion sequence (is) of mycobacterium xenopi has been isolated and sequenced. this 1323 bp element, designated is1395, is present in up to 18 copies in the m. xenopi genome and may be harboured in an m. xenopi extrachromosomal element. it encodes a putative transposase of 415 amino acids which displays sequence homology to the staphylococcus aureus is256 family. members of this class of elements have been described in the genus mycobacterium-for example is1081 is present in the m. tubercul ...19968828212
mycobacterium xenopi--an unusual presentation as tenosynovitis of the wrist in an immunocompetent patient.mycobacterium xenopi is an atypical acid-fast bacillus which may colonize tap water supplies. it typically causes pulmonary infection, particularly in patients with pre-existing lung damage, and non-pulmonary involvement is rare. we describe the first reported case of tenosynovitis due to this organism in an immunocompetent male patient.19968883442
[atypical mycobacteriosis (mycobacterium xenopi) with "initial aggravation"].a 66-year-old man was admitted to nara medical university hospital because of sputum production and fevre. a chest x-ray film obtained on admission revealed many cysts and an infiltrative shadow in the right upper lung field. the patient was treated with antimycobacterial drugs (isoniazid 400 mg, streptomycin 0.75 g, and rifampicin 450 mg) because acid-fast bacilli were detected in his sputum. although the symptoms and laboratory data improved, a new infiltrative shadow developed in the right lo ...19968937151
atypical mycobacteriosis as a complication of talc pneumoconiosis.a 57 year old man, receiving compensation for talc pneumoconiosis since 1977, was admitted to hospital for the first time in 1987, with symptoms of weight loss, fever, dyspnoea and productive cough. a chest roentgenogram showed bilateral cavitation. two years later, mycobacterium xenopi was found in sputum cultures. despite specific oral antibiotherapy, the patient's health deteriorated and he died in 1990. to the best of our knowledge, this is the first reported case of an association of talcos ...19968866607
mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic mycobacterium.a new rapidly growing, scotochromogenic mycobacterium was isolated from urine. this strain is thermophilic (it grows at 65 degrees c), tolerates 5% nacl, and was unable to utilize any of the sugars tested or citrate or to take up iron. the isolate splits benzamide, urea, nicotinamide, and pyrazinamide and is sensitive to streptomycin, ethambutol, cycloserine, ciprofloxacin, and chlarithromycin but resistant to isoniazid, rifampin, and prothionamide. these characteristics clearly place this organ ...19978995808
osteitis of the calcaneus caused by mycobacterium xenopi. 19979002109
efficacy of combined clarithromycin and sparfloxacin therapy in a patient with discitis: due to mycobacterium xenopi. 19979051864
pneumonia and bacteremia due to mycobacterium celatum masquerading as mycobacterium xenopi in patients with aids: an underdiagnosed problem?a newly described species of mycobacteria, mycobacterium celatum, has rarely been reported as a cause of localized and disseminated human disease and can easily be misidentified as mycobacterium xenopi when identification is based on biochemical testing alone. we report two cases of pneumonia and bacteremia due to m. celatum in patients with aids. in one case, diagnosis and initiation of therapy were delayed by misidentification of the infecting organism as m. xenopi. genomic analysis or high-pe ...19979114137
mycobacterium xenopi: innocent bystander or emerging pathogen?mycobacterium xenopi is a recognized cause of smoldering pulmonary disease in patients with chronic lung disease. this organism is frequently isolated from respiratory specimens from individuals infected with human immunodeficiency virus (hiv) and is often considered nonpathogenic. cases of pulmonary and disseminated m. xenopi disease have been described in patients with hiv infection and other immunodeficiencies. many physicians are unaware of the clinical significance of m. xenopi isolation. w ...19979114152
is mycobacterium xenopi an emerging pathogen? 19979114153
comparison of a new insertion element, is1407, with established molecular markers for the characterization of mycobacterium celatum.genomic analyses of 18 mycobacterium celatum strains obtained from different patients in three countries (united states, united kingdom, and france) were performed; the methods used in this study were restriction fragment length polymorphism (rflp) analysis, pulsed-field gel electrophoresis (pfge) analysis, and pcr restriction analysis (pra) of the hsp-65 gene. a new insertion sequence, is1407 (genbank accession no. x97307), belonging to the is256 family, was identified in m. celatum type 1 stra ...19979226894
mycobacterium xenopi infection in patients with human immunodeficiency virus infection.mycobacterium xenopi is one of the most frequently isolated nontuberculous mycobacteria in ontario, canada. we reviewed the records of 28 human immunodeficiency virus (hiv)-infected patients from whom m. xenopi was isolated between 1982 and 1995. m. xenopi was recovered from respiratory specimens from 24 patients, most of whom had clinical and radiographic evidence of pulmonary disease. however, coexistent pulmonary infection due to other pathogens was found in 17 patients: pneumocystis carinii ...19979332511
the mycobacterium xenopi gyra protein splicing element: characterization of a minimal intein.the 198-amino-acid in-frame insertion in the gyra gene of mycobacterium xenopi is the smallest known naturally occurring active protein splicing element (intein). comparison with other mycobacterial gyra inteins suggests that the m. xenopi intein underwent a complex series of events including (i) removal of 222 amino acids that encompass most of the central intein domain, and (ii) addition of a linker of unrelated residues. this naturally occurring genetic rearrangement is a representative chara ...19979335286
[manifestations, diagnosis and treatment of non-tuberculous mycobacterial infections in non-immunodepressed patients].non-tuberculous mycobacteria (ntm) can be the etiologic agents of chronic pulmonary disease, lymphadenitis, skin and soft-tissue infection and disseminated disease in non-immunocompromised patients. the recognition of disease needs repeated isolation of the ntm from bronchopulmonary secretions or from tissue biopsies, and its identification by specific laboratory methods. a wide spectrum of clinical presentations and severity of disease can be found, from spontaneous healing to progressive and d ...19979496598
[lessons in medicine: apropos of a modern epidemic of mycobacterium xenopi spondylodiscitis]. 19979499984
characterization of large linear plasmids in mycobacteria.linear plasmids were found in mycobacterium xenopi, m. branderi, and m. celatum. these elements represented a wide size range (from 20 to 320 kb), had streptomyces-like terminal structures, and defined five hybridization groups. cross-hybridization and common restriction fragment length polymorphism patterns of plasmids from the different species suggest either genetic exchange or a common ancestry of the species.19979098076
arthritis and spondylodiscitis caused by mycobacterium xenopi in a patient with systemic lupus erythematosus. 19979376981
mycobacterium xenopi osteomyelitis. 19979380461
nontuberculous mycobacterial bursitis and arthritis of the shoulder.a 56-year-old woman with systemic lupus erythematosus developed septic arthritis and bursitis of the left shoulder due to an atypical mycobacterium, m. xenopi. plain radiography, ultrasound (us), and mri were performed. articular disease by tuberculous and nontuberculous mycobacteria have similar presentations, clinically as well as radiologically, and have to be differentiated from other chronic bacterial or fungal infections, pigmented villonodular synovitis, rheumatoid arthritis, gout, hemoph ...19989507608
standardized bactec method to measure clarithromycin susceptibility of mycobacterium genavense.a standardized clarithromycin susceptibility test for mycobacterium genavense is reported. the bactec radiometric broth dilution test method recommended for mycobacterium avium complex was modified to develop a reliable and reproducible procedure. test development involved optimization of medium ph and inoculum densities for antibiotic vials as well as growth control vials. mic control organisms included mycobacterium simiae, mycobacterium avium, and mycobacterium xenopi. growth control vials re ...19989508306
mycobacterium xenopi pulmonary infection: evaluation with ct.our goal was to describe the ct appearances of mycobacterium xenopi pulmonary infection.19989530384
mycobacterium xenopi arthritis.osteoarticular mycobacterium xenopi infections are very rare. we describe a patient with dermatomyositis and isolated shoulder arthritis due to m. xenopi. the diagnosis was delayed for 7 months; only 2 of 5 interval joint aspirates grew m. xenopi. the infection responded well to triple antituberculous therapy.19989598912
preliminary results of collapse therapy with plombage for pulmonary disease caused by multidrug-resistant mycobacteria.seven patients underwent collapse therapy with polystyrene sphere plombage for pulmonary disease caused by multidrug-resistant mycobacteria. four patients were infected with multidrug-resistant strains of mycobacterium tuberculosis, two with mycobacterium xenopi, one with mycobacterium avium. all patients were heavily pretreated before surgery, had extensive, bilateral cavitary disease and were considered unsuitable for resection because of extensive disease or functional respiratory impairment. ...19989603145
[a case of pulmonary infection with mycobacterium xenopi].a 42-year-old man with pulmonary disease caused by mcobacterium xenopi is described. he had a history of alcohol use of over 20 years. he was admitted to another hospital for evaluation of an abnormal chest shadow. a chest x-ray showed a cavitary shadow with infiltration in the right upper lung field. acid-fast organisms were recognized in sputum and m. xenopi was identified by dna-dna hybridization. although he had been treated with isoniazid, rifampicin, and ethanbutol for 5 years, sputum rema ...19989611983
clinical and radiographic manifestations of uncommon pulmonary nontuberculous mycobacterial disease in aids patients.to determine the clinical and radiographic findings of nontuberculous mycobacteria (ntm) other than mycobacterium avium complex (mac) and mycobacterium kansasii in aids compared with non-aids patients.19989674460
mycobacterial linear plasmids have an invertron-like structure related to other linear replicons in actinomycetes.the authors previously identified large plasmids in mycobacterium xenopi, m. branderi and m. celatum which appeared to have a linear topology. this study has confirmed the presence of such linear plasmids in mycobacteria, including m. avium, and demonstrated that the ends of these replicons are covalently bound with protein(s), suggesting an invertron-like structure. the termini of one 25 kb plasmid, designated pclp, from m. celatum were cloned and the first 500 bp of each terminus were sequence ...19989695931
mycobacterium xenopi in hiv-infected patients: an emerging pathogen.mycobacterium xenopi is associated with pulmonary disease in patients with loss of local or general host defence.19989764786
[parotitis due to mycobacterium and hiv infection]. 19989793160
radiographic findings in patients with acquired immunodeficiency syndrome, pulmonary infection, and microbiologic evidence of mycobacterium xenopi.the authors studied radiographs and clinical histories of 29 patients with acquired immunodeficiency syndrome, symptoms of pulmonary infection, and simultaneous microbiologic evidence of mycobacterium xenopi in the respiratory tract. the presence, nature, and distribution of radiographic abnormalities were determined and analyzed in accord with clinical information. in 26 (90%) patients, m. xenopi was the only microorganism that could be isolated. chest radiographs were normal in 13 patients (45 ...19989799136
infections due to nontuberculous mycobacteria in children with leukemia.we reviewed the spectrum of infections due to nontuberculous mycobacteria (ntm) in children with leukemia. three children acquired such infections. one patient developed pneumonia after the cessation of chemotherapy when mycobacterium xenopi was identified in his lung biopsy specimen. he required 2 years of treatment with antituberculous agents and clarithromycin. cultures of central and peripheral blood specimens from two patients yielded mycobacterium fortuitum and mycobacterium chelonae, resp ...19989827274
[epidemiologic and microbiologic aspects of mycobacteriosis in slovakia--m. xenopi].the first mycobacterioses caused by m. xenopi in slovakia were discovered in the year 1979. gradually they became the most often occurring mycobacterioses caused by facultative pathogenic mycobacteria. the purpose of investigation is to describe epidemiological situation of diseases caused by m. xenopi in slovakia and to confront it with the situation in chosen developed countries. the methodology of this paper is based upon surveillance of tuberculosis as applied on mycobacterioses. during the ...19989919760
[pulmonary infection by mycobacterium xenopi in a patient with positive hiv]. 199810079546
crystal structure of gyra intein from mycobacterium xenopi reveals structural basis of protein splicing.several genes from prokaryotes and lower eukaryotes have been found to contain an in-frame open reading frame, which encodes for an internal protein (intein). post-translationally, the internal polypeptide auto-splices and ligates the external sequences to yield a functional external protein (extein) and an intein. most, but not all inteins, contain, apart from a splicing domain, a separate endonucleolytic domain that enables them to maintain their presence by a homing mechanism. we report here ...19989437427
use of microscopic morphology in smears prepared from radiometric cultures for presumptive identification of mycobacterium tuberculosis complex, mycobacterium avium complex, mycobacterium kansasii, and mycobacterium xenopi.the aim of this study was to assess the feasibility of a method for presumptive identification of mycobacteria, based on the morphology in smears prepared from radiometric bactec-positive cultures (becton dickinson, usa) and to select the appropriate dna probe (accuprobe; gen probe, usa). the smear morphology of acid-fast bacilli was evaluated in 468 positive cultures from clinical samples: 313 mycobacterium tuberculosis complex, 67 mycobacterium avium complex, 32 mycobacterium kansasii, 49 myco ...19989764552
[osteoarticular mycobacterium xenopi infection].mycobacterium xenopi is a potential pathogen for man and can cause bone and joint infections, particularly spondylodiscitis. most cases of infection occur in fragilized patients and are found more and more often in aids patients.19989767883
primary psoas muscle abscess due to mycobacterium xenopi. 19989455560
inteins in mycobacterial gyra are a taxonomic character.the a subunit of dna gyrase in mycobacteria is frequently subjected to splicing events as its gene, gyra, harbours an insertion encoding an intein. investigation of a number of different isolates of mycobacterium kansasii, mycobacterium malmoense, mycobacterium marinum, mycobacterium ulcerans and mycobacterium xenopi demonstrated that the presence of gyra inteins is not random but a taxonomic character specific for a given taxon at a species or subspecies level.19989493395
no news is good news: opportunistic infections.the 38th interscience conference on antimicrobial agents and chemotherapy (icaac) was notable for not having any oral sessions on opportunistic infections. this is an indication of the enormous success of combination antiretroviral therapy, which has greatly reduced the occurrences of opportunistic infections. hepatitis c was discussed at the conference, but it was treated as a co-morbid condition rather than an opportunistic infection. two studies addressed the question of stopping pneumocystis ...199811366129
lymphadenitis and peritonitis caused by mycobacterium xenopi in a cat. 199812075548
[mycobacterium xenopi, a newcomer?]. 199811767362
[changes in the kidneys in patients with successive findings of mycobacterium xenopi and mycobacterium fortuitum in the urine--report of 16 cases]].environmental or mott (mycobacteria other than tubercle bacilli) mycobacteria are found in both living environment and most of the food we consume. these mycobacteria can induce a disease in humans, although they rarely do. there are a few reports of urogenital infections caused by these bacteria. this is a report of 16 patients with successive findings of mycobacterium xenopi and mycobacterium fortuitum in the urine.199910624381
mycobacterium xenopi lung infection. 199910678220
purification of proteins fused to either the amino or carboxy terminus of the mycobacterium xenopi gyrase a intein.the mycobacterium xenopi gyrase a mini-intein has been engineered to yield a controllable n-terminal or c-terminal, single-splice-junction autocleavage element. when combined with an affinity tag, these modified mini-inteins can be used to purify target proteins after a single combined chromatography/cleavage step. cleavage at the intein n terminus was induced with thiol reagents, while cleavage at the intein c terminus was induced by a temperature shift to 16 degrees-25 degrees c. different pre ...199910407673
[clinical significance of the isolation of mycobacterium xenopi]. 199910422079
systemic mycobacterioses in aids patients as determined by blood cultures on biphasic medium.bacteremia due to mycobacteria can occur in aids patients in whom a rapid diagnosis is extremely important in order to plan a therapeutic conduct. blood culture of mycobacteria using a biphasic system was set up in the regional laboratories of the adolfo lutz institute, sp (campinas, ribeirão preto, santo andré, santos, são josé do rio preto and sorocaba). during a three year period (1994-97), 1521 blood samples were analyzed from 1336 aids patients, with cd4+ cell count < 100/ml, hematocrit < 3 ...199910425659
non-tuberculous mycobacterial tenosynovitis: a review.the clinical characteristics, outcome and treatment of non-tuberculous mycobacterial tenosynovitis are reviewed. from lesions localized in the hand, 10 different species of non-tuberculous mycobacteria have been reported. the most common are mycobacterium marinum and mycobacterium kansasii. other less frequent organisms are mycobacterium avium complex, mycobacterium szulgai, mycobacterium terrae, mycobacterium fortuitum, mycobacterium chelonae, mycobacterium abscessus, mycobacterium malmoense an ...199910482048
use of bactec mgit 960 for recovery of mycobacteria from clinical specimens: multicenter study.the bactec mgit 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. its performance was compared to those of the radiometric bactec 460 instrument and egg-based lowenstein-jensen medium. an identical volume of sample was inoculated in different media, and incubation was carried out for 6 weeks with the automatic systems and for 8 weeks on solid media. a total of 2,567 specimens obtained from 1,631 patients wer ...199910523555
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