| immunodiffusion studies of ribosomes in classification of mycobacteria and related taxa. | ribosomal preparations consisting of crude ribosomes (cr), 30s subunits (30s) and 16s core particles (16s) from four strains of the species mycobacterium bovis (bcg), mycobacterium fortuitum, mycobacterium phlei and mycobacterium smegmatis were analyzed by immunodiffusion technique for taxonomical purposes. the ribosomal preparations tested contained several interspecies cross-reacting precipitinogens. the number of precipitinogens demonstrated at the homologous reactions was generally larger th ... | 1979 | 109401 |
| immunodiffusion studies of various structural preparations from mycobacterial cells. | various structures and other preparations from mycobacterial cells were analyzed by immunodiffusion. the preparations were obtained from four strains referred to the species mycobacterium bovis (bcg), mycobacterium fortuitum, mycobacterium phlei and mycobacterium smegmatis. they represented cell walls (cw), culture filtrates (cf), artificially disintegrated cell material (xp), protoplasms (pp), crude ribosomes (cr), ribosomal 50s subunits (50s), ribosomal 30s subunits (30s), ribosomal 16s core p ... | 1979 | 109402 |
| restoration of oxidative phosphorylation by purified n,n'-dicyclohexylcarbodiimide-sensitive latent adenosinetriphosphatase from mycobacterium phlei. | the n,n'-dicyclohexylcarbodiimide (dccd)-sensitive latent adenosinetriphosphatase (atpase) (ec 3.6.1.3; atp phosphohydrolase) from mycobacterium phlei has been purified to homogeneity and used to resotre oxidative phosphorylation to detergent-extracted membranes. the phosphorylation was inhibited by dccd any by tetraphenylboron and valinomycin. the enzyme was solubilized from the membrane vesicles by treatment with cholate followed by extraction with triton x-100. after partial purification on a ... | 1976 | 135258 |
| trypsin-induced changes in the orientation of latent atpase in protoplast ghosts from mycobacterium phlei. | latent atpase, located on the inner surface of protoplast ghosts of mycobacterium phlei, was unmasked either by trypsin or an impermeable form of trypsin, ethylene maleic anhydride-trypsin. density gradient experiments showed that the ghost preparations remained intact following trypsin treatment. evidence was obtained that 125i-trypsin failed to penetrate the ghost membranes. thus, attempts were made to determine whether the atpase molecule in the ghost membranes is accessible from the outer su ... | 1977 | 144135 |
| affinity labeling of coupling factor-latent atpase from mycobacterium phlei with 2',3'-dialdehyde derivatives of adenosine 5'-triphosphate and adenosine 5'-diphosphate. | | 1979 | 154517 |
| properties of energy-transducing systems in different types of membrane preparations from mycobacterium phlei--preparation, resolution, and reconstitution. | | 1979 | 156832 |
| stimulation of proline transport by cupric ion in membrane vesicles from mycobacterium phlei. | | 1976 | 178313 |
| adenosine 3',5'-monophosphate in mycobacterium phlei and mycobacterium tuberculosis h37ra. | adenosine 3',5'-monophosphate (camp) is present in slow growing as well as fast growing mycobacteria. apparently there does not seem to be any direct relationship between either intra- or extracellular camp content with the growth rate of bacilli. as compared to that of e. coli grown on a similar energy source, camp content is much higher in mycobacteria. camp content inside the cells remains unaltered throughout the growth period and this may be due to lack of complete utilization of the major ... | 1976 | 196160 |
| induction of a morphological mutant in mycobacterium phlei by gamma-radiation and ethyl methanesulfonate. | | 1978 | 207206 |
| purification, physicochemical properties, and localization of cytochrome c in energy-transducing membranes from mycobacterium phlei. | | 1979 | 220917 |
| an adenosine diphosphoglucose glucohydrolase of mycobacterium phlei. | | 1979 | 226469 |
| specificity of isoniazid on growth inhibition and competition for an oxidized nicotinamide adenine dinucleotide regulatory site on the electron transport pathway in mycobacterium phlei. | the mechanism of action of isoniazid (inh) on saprophytic and atypical mycobacteria is thought to be different from that on mycobacterium tuberculosis because higher concentrations are required to be effective in these species. in this investigation, m. phlei was inhibited by inh at a concentration of 25 mug/ml. benzoic acid hydrazide (bzh) and nicotinic acid hydrazide (nah) were inhibitory at levels of 300 and 500 mug/ml, respectively. inhibition by these compounds was not inoculum dependent. a ... | 1977 | 197885 |
| alterations in lipid constituents during growth of mycobacterium smegmatis cdc 46 and mycobacterium phlei atcc 354. | phospholipids of mycobacterium phlei atcc 354 and mycobacterium smegmatis cdc 46 consist of cardiolipin, phosphatidyl ethanolamine, tri-acylated dimannophosphoinositide, tetra-acylated dimannophosphoinositide and tetra-acylated pentamannosphosphoinositide. a comparative study of lipid patterns of m. phlei atcc 354 and of m. smegmatis cdc 46 in relation to age of culture revealed higher total lipid level and increased activity of malate-vitamin k reductase, a phospholipid requiring enzyme, during ... | 1976 | 196159 |
| on the antigenicity of phosphatidylinositolmannosides of mycobacterium phlei atcc 354. | | 1975 | 176114 |
| uptake of radioactive dopa by mycobacterium leprae in vitro. | our previous studies demonstrated that mycobacterium leprae contains a characteristic o-diphenoloxidase which converts a variety of phenolic compounds to quinones in vitro. this enzyme was not present in any other mycobacteria tested. the results reported here deal with the uptake and binding of radioactive dopa by m. leprae. the leprosy bacilli incubated with tritium-labelled dopa, readily took up the substrate. the binding of dopa by the bacilli was markedly inhibited by diethyldithiocarbamate ... | 1975 | 171542 |
| quantitation of seven elements in mycobacterium phlei and mycobacterium bovis by neutron activation analysis. | quantitative determination of the elements potassium, sodium, manganese, magnesium, iron, cobalt and zinc was performed in mycobacteria by neutron activation analysis. mycobacterium phlei atcc 19 249 at different phase of growth (4, 8, 13, 23 and 37 days old cultures), and 14 days old mycobacterium bovis bcg cultures and uninoculated semi-synthetic sauton culture media were examined. the elements studied could be divided into three groups; sodium, potassium and magnesium could be regarded as maj ... | 1977 | 341656 |
| [nucleotide makeup of the dna of fast-growing mycobacteria and related microorganisms]. | the melting points of dna were used to determine the nucleotide composition of dna from the following microorganisms with a high growth rate: mycobacterium phlei, m. smegmatis, m. fortuitum, nocardia asteroides, the organism of the "rhodochrous"--n. opaca group, and organisms related to strain "mycobacterium" rhodochrous atcc 13808. m. fortuitum had a higher mol% g+c (67.5--69.5) than m. phlei (66.9--67.5) and m. smegnatis (64.4--68.1); the strains of m. smegmatis were characterized by the most ... | 1978 | 351339 |
| the variable response of bacteria to excess ferric iron in host tissues. | the enhancement by exogenous ferric iron, both systemic and local, of the infectivity of 120 strains of bacteria, representing 17 genera, was measured in the skin of guinea-pigs. systemic iron enhanced only 23% of 115 strains, and local iron 49% of 71 strains. systemic iron, by an apparently anti-inflammatory action, depressed the size of lesions produced by 27 of the non-enhanced strains from nine of the genera tested. for most strains, the degree of enhancement was small, ranging from 2- to 8- ... | 1979 | 372534 |
| multiple forms of cytochrome b in mycobacterium phlei: kinetics of reduction. | the kinetics of reduction of the b-type cytochromes in the electron transport particles (etp) from mycobacterium phlei were studied with nicotinamide adenine dinucleotide, reduced form (nadh) or succinate as electron donors. there appeared to be three active cytochromes b in the etp,bs563 and bs559, which were reducible by either substrate, and bn563, which was reducible by nadh but not by succinate. in the presence of adenosine 5'-triphosphate, a substantial increase in b563 reduction was obser ... | 1975 | 166977 |
| [dehydrogenases reducing nad+ in the presence of d (-) 3- phosphoglycerate in mycobacteria (bcg, h37ra, m. phlei)]. | referring to the elution volume on a sephadex g-150 column only one specific peak is obtained, the same for the bcg, h37ra and mycobacterium phlei strains grown on sauton synthetic medium. some properties of these partially purified dehydrogenases are studied (conservation and dialysis in media of different salt concentrations, equilibrium constant, km, heat stability). all enzyme preparations from tubercle bacilli (bcg, h37ra) are readily inactivated by heat and are very unstable in solutions o ... | 1979 | 385063 |
| active transport of calcium in membrane vesicles from mycobacterium phlei. | active transport of calcium ions has been demonstrated in inside-out membrane vesicles from mycobacterium phlei mediated by respiratory linked substrates as well as by atp hydrolysis. the uptake of calcium exhibited an apparent km of 80 microm and v of 16.6 nmol calcium uptake x min-1 x mg protein-1. a fortyfold concentration gradient for calcium ions was calculated for both the atp-induced and the respiration-induced transport of calcium. removal of coupling-factor-latent atpase resulted in the ... | 1979 | 159818 |
| [enzymatic differences in mycobacteria. ribitol dehydrogenase]. | contrary to the tubercle bacilli (h37ra, bcg), mycobacterium phlei has a ribitol-nad dehydrogenase (that also oxidizes, although to a lesser extent, erythritol and glycerol). this difference is observed with the bacteria grown on sauton's medium, as well as after their adaptation to ribitol. the extracts of all these mycobacteria reduce, nadp in the presence of glycerol, ribitol or erythritol, though very slowly. | 1977 | 408036 |
| limited proteolysis of coupling factor-latent atpase from mycobacterium phlei. effects of different enzymes and modifying agents. | the activation of the coupling factor-latent atpase enzyme by tryptic proteolysis may resemble the activation of many proenzymes by limited proteolysis. the beta (53 000 dalton) subunit of solubilized coupling factor-latent atpase from mycobacterium phlei was selectively lost in some trypsin-treated samples. since a concomitant loss of atpase activity was not observed, the beta subunit may not be essential for atpase catalytic activity. treatment of solubilized coupling factor with chymotrypsin ... | 1979 | 157159 |
| tryptic proteolysis of coupling factor-latent atpase from mycobacterium phlei. theoretical modeling of structure-function relationships. | trypsin treatment of solubilized coupling factor-latent atpase from mycobacterium phlei alters its subunit structure and functional properties. this coupling factor exhibits atpase activity following trypsin treatment. concurrently, both the ability of the enzyme to rebind to membranes depleted of coupling factor and its capacity for coupled phosphorylation are lost. the native alpha (64 000 dalton) subunit undergoes limited proteolytic digestion, and the delta (14 000 dalton) subunit is partial ... | 1979 | 157157 |
| purification and characteristics of hydrophobic membrane protein(s) required for dccd sensitivity of atpase in mycobacterium phlei. | the energy-transducing n,n'-dicyclohexylcarbodiimide-sensitive (dccd-sensitive) atpase complex consists of two parts, a soluble catalytic protein (f1), and an intrinsic membrane protein (f0). the bacterial coupling factor complex, bcf0-bcf1, has recently been purified from mycobacterium phlei, and used to reconstitute oxidative phosphorylation in detergent-extracted membranes. the bcf0 moiety has been purified by being recovered from the purified bcf0-bcf1 complex by affinity chromatography. bcf ... | 1978 | 153436 |
| asymmetric distribution of phospholipids in membranes from mycobacterium phlei. | | 1979 | 507841 |
| effects of trypsin treatment on the structure and function of solubilized coupling factor-latent atpase from mycobacterium phlei. | | 1977 | 140685 |
| purification and properties of membrane-bound coupling factor-latent atpase from mycobacterium phlei. | the membrane bound coupling factor-latent atpase was solubilized from the membrane vesicles of mycobacterium phlei by using 0.25 m sucrose or low ionic strength buffer. purification of the solubilized enzyme by use of sepharose-adp conjugate gel yielded a homogenous preparation of latent atpase which was purified about 216-fold in a single step with an 84% yield. the enzyme exhibits a specific activity of 39 mumoles of atp hydrolyzed per min per mg protein. the purified enzyme exhibits coupling ... | 1975 | 127086 |
| energy-transducing membrane-bound coupling factor-atpase from mycobacterium phlei. i. purification, homogeneity, and properties. | the membrane-bound coupling factor from mycobacterium phlei was solubilized from membrane vesicles by washing with low ionic strength buffer or 0.25 m sucrose. the solubilized enzyme exhibited coupling factor, latent atpase, and succinate oxidation-stimulating activity. purification by affinity chromatography using sepharose coupled to adp yielded a homogeneous preparation of latent atpase which was purified about 200-fold with an 84% yield in a single step. purified latent atpase exhibited coup ... | 1975 | 125754 |
| effect of phospholipase a on active transport of amino acids with membrane vesicles of mycobacterium phlei. | active transport of proline remained unaffected in phospholipase a-treated electron transport particles from mycobacterium phlei. however, the steady state level of proline was reduced 50 to 60% in phospholipase a-treated depleted electron transport particles that were devoid of membrane-bound coupling factor-latent atpase activity. the decrease in the uptake of proline in the phospholipase a-treated depleted electron transport particles was not due to a change in the apparent k-m for proline, b ... | 1975 | 123919 |
| the specificity of the fluorescent antibody test using the sera of rabbits inoculated with strains of myocobacteria. | sera from experimentally infected rabbits were used to test the specificity of the fluorescent antibody test. it was possible using mono-specific sera to differentiate antigenically mycobacterium phlei, m fortuitum, m smegmatis, m avium, m intracellulare, m bovis (bcg) and m johnei. the cross-reactivity within the m avium and m intracellulare group was such that one antigen from these groups would detect infection within that group and exclude m johnei infection. the m phlei growth factor indepe ... | 1976 | 56767 |
| alteration of mycobacterium phlei membrane structure by freezing and thawing: reversal by heating. | | 1975 | 49172 |
| mycobacterium phlei pn-bb colonies: a morphological characterisation by scanning and transmission electron microscopy. | colonies of a non-acid-fast mutant of mycobacterium phlei--termed "pn--bb"--were examined by scanning electron microscopy of gold-sputted whole colonies and by transmission electron microscopy of thin sections. inspection of the colonies before and after preparation showed that the fixed colonies had retained their original appearance. colonies are about 2-5 mm in height and width. they are made up of converging ridges forming a cone. these ridges consists of rounded bodies which are made up of ... | 1978 | 666218 |
| antibacterial activity of n-(beta-styryl) formamides related to tuberin. | a series of para-substituted n-(beta-styryl)formamides, analogues of tuberin (4a), has been prepared and assayed for antibacterial activity. the methylthio, ethoxy, and methyl analogues 4e, 4j, and 4t were about twice as active as tuberin against mycobacterium phlei. although tuberin lacks activity against staphylococcus aureus, several of the analogues described were found to inhibit this organism. the phenyl group of tuberin is not a prerequisite for activity since analogues based on naphthyl ... | 1978 | 671458 |
| isolation and properties of naphthoate synthetase from mycobacterium phlei. | | 1978 | 677895 |
| polyol dehydrogenases in mycobacteria (author's transl). | contrary to the the tubercle bacilli (h37ra, bcg), mycobacterium phlei, grown on sauton medium, formed the nad+ dependent dehydrogenases that catalyse the oxidation of ribitol, sorbitol and mannitol. these enzymes were separated by chromatography on deae-cellulose and sephadex g-200. in the present work we have principally studied the ribitol dehydrogenase. all the experiments for induction of the ribitol dehydrogenase in h37ra or bcg were negative; whereas after the adaptation of m. phlei to ri ... | 1978 | 29546 |
| purification and properties of beta-hydroxybutyrate dehydrogenase from mycobacterium phlei atcc354. | beta-hydroxybutyrate dehydrogenase (ec 1.1.1.30) was purified 145-fold from mycobacterium phlei atcc354 by ammonium sulphate fractionation and deae-cellulose chromatography. the ph optima for oxidation and reduction reactions were 8.4 and 6.8 respectively. the purified enzyme was specific for nad, nadh, acetoacetate and d(-)-beta-hydroxybutyrate. km values for dl-beta-hydroxybutyrate and nad were 7.4 mm and 0.66 mm respectively. the enzyme was inactivated by mercurial thiol inhibitors and by hea ... | 1978 | 24083 |
| purification and properties of a triacylglycerol lipase from mycobacterium phlei. | in order to study the metabolism of triacylglycerol in mycobacteria, an intracellular particulate triacylglycerol lipase (ec 3.1.1.3) was purified 800-fold from stationary phase cells of mycobacterium phlei. extraction of whole cell suspensions with 5% triton x-100, followed by ion-exchange chromatography of the extract on two successive deae-cellulose columns produced a preparation which was nearly homogeneous by the criterion of analytical isoelectric focusing in acrylamide gels (one band, pi. ... | 1977 | 18200 |
| separation of c50--60 and c70--80 mycolic acid molecular species and their changes by growth temperatures in mycobacterium phlei. | | 1978 | 720589 |
| synthesis and quantitative structure--activity relationships of some antibacterial 3-formylrifamycin sv n-(4-substituted phenyl)piperazinoacethydrazones. | a series of 14 3-formylrifamycin sv n-(4-substituted phenyl)piperazinoacethydrazones has been synthesized and evaluated for their antimicrobial activity. the compounds were found active against bacillus subtilis, staphylococcus aureus, mycobacterium phlei, and mycobacterium tuberculosis but not as active as rifampin. the compounds also exhibited significant activity against clostridium perfringens and in this bacterial system some were more active than rifampin. the qsar showed that the activity ... | 1978 | 722738 |
| binding of nucleotides to purified coupling factor-latent atpase from mycobacterium phlei. | binding studies of various nucleotides to the purified coupling factor-latent atpase from mycobacterium phlei have been carried out using gel filtration, equilibrium dialysis, and ultrafiltration methods. the purified latent atpase binds 3 mol of adp per mol of the enzyme with an apparent dissociation constant of 68 mum. binding of nucleotides occurred in the decreasing order: adp, epsilon-atp, epsilon-adp, udp, adenyl-5'-yl imidodiphosphate (amp-p(nh)p), idp, and adenosine 5'-(alpha,beta-methyl ... | 1977 | 14131 |
| nicotinamide adenine dinucleotide -- independent formate dehydrogenase in mycobacterium phlei. | formate dehydrogenase activity (ec 1.2.1.2) has been demonstrated in cell-free preparations of mycobacterium phlei by following the reduction of 2,6 dichlorophenolindophenol. thiazolyl blue tetrazolium, or equine cytochrome c. the reduction of equine cytochrome c was inhibited by 2-heptyl-4-hydroxyquinoline-n-oxide. neither nicotinamide adenine dinucleotide nor nicotinamide adenine dinucleotide phosphate were reduced by this formate dehydrogenase. the enzyme was constitutive and associated with ... | 1977 | 13920 |
| [phosphotransacetylases from bcg and mycobacterium phlei]. | | 1976 | 788798 |
| purification and properties of l-asparaginase from mycobacterium phlei. | 1. l-asparaginase from m. phlei was purified about 170-fold with an 11% yield. the purification procedure consisted of: fractionation with ammonium sulphate; adsorption of contaminating proteins on calcium phosphate gel; chromatography on sephadex g-150 and deae-cellulose. the specific activity of the final preparation was 32.6 i.u./mg protein. 2. molecular weight of the enzyme as determined by sephadex g-100 filtration amounted to 126 000. optimum ph was 8.8-9.2. the enzyme did not hydrolyse l- ... | 1976 | 7091 |
| ultraviolet susceptibility of bcg and virulent tubercle bacilli. | to test the effectiveness of irradiating the upper air of a room with ultraviolet light at reducing the concentration of airborne tubercle bacilli, the susceptibility to the germicidal effects of ultraviolet light, z, was determined for various mycobacteria. virulent tubercle bacilli and bacille calmette-guérin (bcg) were equally susceptible to ultraviolet radiation, whereas mycobacterium phlei had 10 times their resistance (z, approximately one-tenth that for m. tuberculosis). the effectiveness ... | 1976 | 817628 |
| effect of metal ions in the culture medium on the stearoyl-coenzyme a desaturase activity of mycobacterium phlei. | a particulate fraction prepared from mycobacterium phlei grown in a metal-deficient medium exhibited a greatly reduced activity of stearoyl-coa desaturase compared to that from normally grown cells. metal deficiency, however, had no effect on the fad-dependent nadph-cytochrome c reductase activity, which has been suggested to participate in the desaturation process. when the cells were grown in the deficient medium supplemented with both fe2+ and mg2+, the desaturase activity was restored to the ... | 1975 | 2587 |
| metabolic fate of cholesteryl methyl ether in mycobacterium phlei. | mycobacterium phlei transformed cholesteryl methyl ether into three metabolites: 3beta-methoxy-dinor-5,17(20)-choladien-22-oic methyl ester (i), 3beta-methoxy-5-androsten-17-one (ii), and 3beta-methoxy-dinor-5-cholen-22-ol (iii). after isolation with thin-layer chromatography, their structures were elucidated by mass, ir and nmr spectroscopy. compound ii was the major product. compounds i and iii were products of various side reactions. in the presence of 8-hydroxyquinoline that inhibits degrada ... | 1975 | 818881 |
| analysis of bacterial biotin-proteins. | the biotin-protein populations in several bacterial strains were analyzed by solubilization of [3h]biotin-labeled cells with sodium dodecylsulfate followed by electrophoresis on polyacrylamide gels containing the detergent. a variety of patterns of biotin-labeled polypeptide chains was seen, ranging from a single biotin-protein in escherichia coli, corresponding to the biotin carboxyl carrier protein component of acetyl-coa carboxylase, to multiple species in enterobacter aerogenes, pseudomonas ... | 1975 | 235315 |
| variations in the pathways of malate oxidation and phosphorylation in different species of mycobacteria. | mycobacterium tuberculosis h37rv, the slow-growing human pathogenic strain of tubercle bacilli and mycobacterium smegmatis and mycobacterium phlei, the fast-growing saprophytes, have shown variations regarding the type of dehydrogenase that initiates malate oxidation in the respiratory chain. m. tuberculosis h37rv is characterized by having a malate oxidase system (designated malnad pathway) in which malate oxidation is mediated by the nad+-dependent malate dehydrogenase (ec 1.1.1.37) but not by ... | 1975 | 234747 |
| effect of nicotinamide adenine dinucleotide on the membrane-associated reduced nicotinamide adenine dinucleotide oxidase of mycobacterium phlei. | nad+ had a biphasic effect on the nadh oxidase activity in electron transport particles from mycobacterium phlei. the oxidase was inhibited competitively by nad+ at concentrations above 0.05 mm. nad+ in concentrations from 0.02 to 0.05 mm resulted in maximum stimulation of both nadh oxidation and oxygen uptake with concentrations of substrate both above and below the apparent k-m. oxygen uptake and cyanide sensitivity indicated that the nad+ stimulatory effect was linked to the terminal respirat ... | 1975 | 234463 |
| effect of oxygen tension on oxidative phosphorylation in mycobacterium phlei. | | 1976 | 955666 |
| effect of phospholipase a on the structure and functions of membrane vesicles from mycobacterium phlei. | the phospholipid composition of the electron transport particles and coupling factor-depleted electron transport particles of mycobacterium phlei are the same, but they differ in contents. the accessibility of partially purified phospholipase a to these membrane phospholipids was found to be different. treatment of membranes of mycobacterium phlei with phospholipase a impairs the rate of oxidation as well as phosphorylation. the inhibition of phosphorylation can be reversed by washing the membra ... | 1975 | 236299 |
| l-asparaginase activity of mycobacterium phlei under various growth conditions. | seven mycobacterium strains were grown statically on salts-glycerol-asparagine (sauton) or on salts-glucose-glutamate (sym) media. at desired time of incubation, the bacteria were washed with water, disintegrated with powdered corundum and in resulting cell-free extracts l-asparaginase activity was determined by the conway method. the majority of experiments were performed on m. phlei which exhibited considerable rise in l-asparaginase activity with increasing age of the culture. this change did ... | 1975 | 242189 |
| fatty acid synthases from mycobacterium phlei. | | 1975 | 1121296 |
| methylated polysaccharide activators of fatty acid synthase from mycobacterium phlei. | | 1975 | 1121297 |
| different binding sites for entry and exit of amino acids in whole cells of mycobacterium phlei. | on the basis of mutual inhibition of uptake with different amino acids in whole cells of mycobacterium phlei, it was demonstrated that the binding site of proline was different from those of all other amino acids studied. other groups of amino acids share a common binding site: lysine, histidine, and arginine; valine, leucine, and isoleucine; tryptophan, tyrosine, and phenylalanine; glutamic acid and aspartic acid. the exit and entry processes were studied for proline, glutamine, and glutamic ac ... | 1977 | 263821 |
| lactate oxygenase of mycobacterium phlei. | | 1975 | 1128272 |
| resolution and reconstitution of active transport of calcium by a protein(s) from mycobacterium phlei. | membrane protein(s) responsible for the active transport of calcium in membrane vesicles from mycobacterium phlei have been solubilized from membranes by sodium cholate treatment and partially purified using a hydrophobic resin. reconstitution of calcium transport was demonstrated by reconstitution of detergent extracted membranes with the partially purified protein. the uptake of calcium in the reconstituted system was sensitive to proton-conducting uncouplers. liposomes prepared with partially ... | 1979 | 378992 |
| mycobacterial antigens relating to experimental pulmonary cavity formation. | lipid-protein mixtures were obtained from 2 strains of mycobacteria, and their cavity-forming activities were examined in rabbit lungs. the mixtures were separated into lipid and protein fractions by gel filtration on sephadex lh-20 column. neither lipid nor protein fraction alone had cavity-forming activity; however, restoration of the cavity-forming activity was observed by recombining the fractions. the activity was also reconstructed by combining the protein fraction with cell walls of bacil ... | 1977 | 403839 |
| isolation, purification, and reconstitution of a proline carrier protein from mycobacterium phlei. | membrane vesicles from mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. the transport of proline is na+ dependent and required substrate oxidation. a proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and triton x-100. electron microscopic observation of the detergent-treated membrane vesicles showed that they are closed structures. the detergent-extracted proteins were purified by means of sucrose density gradient ... | 1979 | 444450 |
| various antigenic reactivities in delayed hypersensitivity among crystalline proteins from mycobacterium phlei. | comparisons were made of the delayed-type skin reactivity of 6 crystalline proteins purified from the cell extract of mycobacterium phlei in guinea pigs sensitized with whole cells of the heat-killed bacillus. these highly purified proteins elicited varying degrees of cutaneous reaction. the most active protein had almost the same reactivity as purified protein derivative prepared from the culture filtrate of mycobacterium phlei. on the other hand, the weakest protein did not elicit a marked cut ... | 1979 | 484936 |
| menaquinone (vitamin k2) biosynthesis: conversion of o-succinylbenzoic acid to 1,4-dihydroxy-2-naphthoic acid by mycobacterium phlei enzymes. | the coenzyme a (coa) and adenosine 5'-triphosphate-dependent conversion of o-succinylbenzoic acid (4-[2'-carboxyphenyl]-4-oxobutyric acid) to 1,4-dihydroxy-2-naphthoic acids is an important step in menaquinone (vitamin k2) biosynthesis. cell-free extracts catalyzing this conversion, obtained from mycobacterium phlei, were separated into three protein fractions by treatment with protamine sulfate. the second fraction (fraction b) and the supernatant (fraction s) alone did not catalyze dihydroxyna ... | 1979 | 500558 |
| comparative studies of the strains pa and pn of mycobacterium phlei leading to their reclassification: examination of lipids and dna, biochemical tests and phage typing. | study of lipid and dna, biochemical tests and phage typing performed on the strain pa previously labelled mycobacterium phlei, lead to the conclusion that this strain belongs to the species m. smegmatis. parallel studies performed on strain pn, isolated from a culture of strain pa, as well as dna homology percentage of the two strains, do not support the assumption that strain pn could have resulted from a mutation of strain pa strain pn produces mycolic acids similar to those found in rhodococc ... | 1979 | 539691 |
| purification of superoxide dismutases from mycobacterium phlei. | | 1975 | 1230719 |
| stereochemistry of a methyl-group rearrangement during the biosynthesis of lanosterol. | 1. (3rs,6r)-[6-2h1,6-3h1,6-14c], (3rs,6s)-[6-2h1,6-3h1,6-14c] and (3rs)-[6-3h1,6-14c]mevalonolactones were synthesised from r-[2h1,3h1,2-14c], s-[2h1,3h1,2-14c] and [3h1,2-14c]acetic acids respectively. 2. each mevalonate was converted into cholesterol by a rat liver preparation. 3. each cholesterol specimen was converted into androsta-1,4-diene-3,17-dione by incubation with mycobacterium phlei in the presence of 2,2'.dipyridyl. each specimen of androsta-1,4-diene-3,17-dione was converted into a ... | 1976 | 1245185 |
| turnover of lipids in mycobacterium smegmatis cdc 46 and mycobacterium phlei atcc 354. | the rates of breakdown and renewal of individual lipids in cultures of mycobacterium smegmatis cdc 46 and mycobacterium phlei atcc 354 were investigated by means of a pulse labelling technique using palmitate-1-14c. the results indicated that in growing cultures of both strains phospholipids were broken down, and cardiolipin had a very rapid turnover. in chase experiments, almost 45% and 40% of the radioactivity of this component were lost respectively from m. smegmatis and m phlei during one ge ... | 1978 | 623497 |
| induction of a morphological mutant in mycobacterium phlei by gamma-radiation and ethyl methanesulfonate. | | 1978 | 625303 |
| effect of tween 80 on lipids of mycobacterium phlei atcc 354. | addition of tween 80 to the growth medium brings about qualitative and quantitative changes in the lipids of mycobacterium phlei atcc 354. the results suggest that tween 80 itself may be a variant in the system. | 1978 | 631247 |
| cupric ion-mediated active transport of amino acids in membrane vesicles of mycobacterium phlei. | | 1978 | 632259 |
| the variations of sensitivity to gamma radiation in different strains of mycobacterium phlei. | | 1978 | 649420 |
| polyclonal b-lymphocyte activation of sheep by mycobacterium phlei against sheep pox virus. | a trypsinized preparation of mycobacterium phlei, non specific stimulator of immunity (nsi), and sheep pox virus (spv) were inoculated in different groups of sheep to activate b-lymphocytes and induce spv neutralizing substance(s). nsi sensitized sheep b-lymphocytes in the presence of nsi or lymphokine elaborated spv neutralizing substance(s). the spv sensitized b-lymphocytes also mediated such neutralizing substance(s). healthy control sheep b-lymphocytes failed to show any appreciable amount o ... | 1992 | 1324887 |
| enhanced production of antibody with specific antigen. | on inoculation of nonspecific stimulator of immunity (nsi), prepared from mycobacterium phlei (m. phlei), simultaneously along with sheep pox virus (spv) in sheep, the recipient has exhibited appreciable level of spv specific antibody as early as on 10th day which reached at peak level on 20th day and remained unaltered on 30th day of postimmunisation as evinced by serum neutralisation test (snt), enzyme linked immunosorbant assay (elisa) indirect, fluorescent antibody technique (fat) indirect, ... | 1992 | 1325943 |
| the replication map of the chromosome of mycobacterium phlei. | it was the aim of the present work to construct the replication map of the chromosome of mycobacterium phlei. the method of mutagenesis of the replication point by n-methyl-n-nitroso-n'-nitroguanidine in synchronously dividing populations and the method of analysis of gene frequency were applied. the order of replication of 19 genes on the chromosome was determined by means of induction of back mutations and forward mutations in auxotrophic mutants pa leu and pa met and in double auxotrophic mut ... | 1978 | 689571 |
| [petroleum-oxidizing microflora of the arctic seas of the ussr]. | active petroleum-oxidizing bacteria of the ussr arctic seas are represented by mycobacterium mucosum (non-colored), mycobacterium phlei and mycobacterium brevicale (red-orange) which inhabit the yenisei bay, the kara sea and the laptev sea. vertical distribution of the petroleum-oxidizing mycobacteria is characterized by substitution of non-coloured forms for coloured ones with depth: "white" strains are found mainly in the surface layer while red and yellow-orange strains are detected in deep w ... | 1978 | 703651 |
| efficacy of various spray disinfectants on irreversible hydrocolloid impressions. | this study evaluated the effectiveness of eight disinfectant sprays on irreversible hydrocolloid impressions contaminated with three microorganisms (staphylococcus aureus, mycobacterium phlei, or bacillus subtilis) or mixed oral flora. alcide ld, omc ii, biocide, and professional lysol spray were relatively ineffective under the test conditions. sporicidin and 0.525% sodium hypochlorite were able to effect a 4-log10 (99.99%) reduction against s aureus only. a 4-log10 reduction in bacterial count ... | 1992 | 1520443 |
| evidence for the incorporation of molecular oxygen, a pathway in biosynthesis of n-glycolylmuramic acid in mycobacterium phlei. | the hypothesis that the biosynthesis of the glycolyl group of muramic acid in the peptidoglycan of mycobacterium phlei is catalyzed by a n-acetyl hydroxylase is strongly supported by the experiments reported in this paper. 18o is incorporated into the n-substituent of muramic acid isolated from the peptidoglycan of m. phlei grown under pure oxygen enriched with the 18o isotope. | 1978 | 718994 |
| a model proteoliposomal system for proline transport using a purified proline carrier protein from mycobacterium phlei. | | 1978 | 736936 |
| mechanism of gram variability in select bacteria. | gram stains were performed on strains of actinomyces bovis, actinomyces viscosus, arthrobacter globiformis, bacillus brevis, butyrivibrio fibrisolvens, clostridium tetani, clostridium thermosaccharolyticum, corynebacterium parvum, mycobacterium phlei, and propionibacterium acnes, using a modified gram regimen that allowed the staining process to be observed by electron microscopy (j. a. davies, g. k. anderson, t. j. beveridge, and h. c. clark, j. bacteriol. 156:837-845, 1983). furthermore, since ... | 1990 | 1689718 |
| immunization against anthrax with bacillus anthracis protective antigen combined with adjuvants. | the protective efficacy of immunization against anthrax with bacillus anthracis protective antigen (pa) combined with different adjuvants was tested in hartley guinea pigs and cba/j and a/j mice. adjuvant components derived from microbial products that were tested included threonyl-muramyl dipeptide (threonyl-mdp); monophosphoryl lipid a (mpl); trehalose dimycolate (tdm); and the delipidated, deproteinized, cell wall skeleton (cws) from either mycobacterium phlei or the bcg strain of mycobacteri ... | 1992 | 1730501 |
| concentration and purification of mycobacteriophages with polyethylene glycol 6000. | experiments were performed to concentrate and purify mycobacteriophages with polyethylene glycol 6000; 6, 9, and 8 per cent polyethylene glycol 6,000 proved to be optimal concentrations for precipitating phages of mycobacterium phlei, mycobacterium smegmatis strain butyricum, and mycobacterium smegmatis strain rabinowitz, respectively. preparative polyethylene glycol reverse gradients were constructed for further concentration and purification of the precipitated phages. with the method describe ... | 1976 | 779553 |
| [microflora of active ooze participating in the decomposition of sulfanilic acid]. | microflora of domestic water can be a source of active ooze adapted to sulphanilic acid. adaptation of the microflora to sulphanilic acid at a concentration of 170-200 mg/l takes 6 to 8 days. the microflora of active ooze, immediately after adaptation, consists mainly of pseudomonas species, ps. denitrificans, ps. fluorescens, ps. striata, ps. putida, etc., and also of achromobacter stutzeri, achromobacter flavum, mycobacterium phlei, mycobacterium mucosum, bacillus mesentericus, bac. cereus, sa ... | 1975 | 808686 |
| nonsporulating bacterial species contain dna sequences homologous to the bacillus spore-specific c-protein gene. | genes for small, acid-soluble spore proteins (sasps) are ubiquitous among the spore-forming bacteria and are expressed only during sporulation. although they perform the function of amino acid storage in spores, the members of the sasp-c multigene family probably serve additional functions, so that similar sequences might be present in non-spore-formers. using the sasp-c gene (ssp-c) as a hybridization probe, restriction digests of whole genomic dna from seven nonsporulating bacterial species we ... | 1991 | 1848527 |
| tumor necrosis factor alpha mediates lethal activity of killed gram-negative and gram-positive bacteria in d-galactosamine-treated mice. | treatment with d-galactosamine increases sensitivity of lipopolysaccharide (lps)-responder mice to the lethal effects of lps, while nonresponder mice remain resistant (m.a. freudenberg, d. keppler, and c. galanos, infect. immun. 51:891-895, 1986). in the present study it is shown that, in contrast to lps, killed gram-negative bacteria (salmonella abortus equi and s. typhimurium) were highly toxic for d-galactosamine-treated lps-responder (c57bl/10 scsn and c3h/hen) and -nonresponder (c57bl/10 sc ... | 1991 | 2037372 |
| mutagenic action of nitrofurans on euglena gracilis and mycobacterium phlei. | there is a pronounced difference between the action of antibiotics and nitrofurans on euglena gracilis. those antibiotics that induce hereditary loss of chloroplasts do so only when they affect dividing cells. on the other hand, nitrofurans induce a mass mutation in both dividing and nondividing cells (under conditions of continuous illumination of cultures). it was found that a breakdown product, 5-nitro-2-furaldehyde, is liberated from furadantin and furoxone. this intermediate is responsible ... | 1976 | 817666 |
| selective enzyme staining procedures for characterization of mycobacterial immunoprecipitates. | selective staining procedures for four different enzymes (malate dehydrogenase, glutamic oxaloacetic transaminase, leucine aminopeptidase and glucose phosphate isomerase) in combination with two-dimensional immunoelectrophoresis were successfully applied to the analysis of antigen preparations from mycobacteria. thus, a precipitate corresponding to malate dehydrogenase could e.g. be demonstrated in multi-linear precipitation patterns of mycobacterium avium, mycobacterium bovis bcg, mycobacterium ... | 1986 | 2417958 |
| studies on the purification and characterization of malate dehydrogenase from mycobacterium phlei. | malate dehydrogenase (l-malate:nad+ oxidoreductase, ec 1.1.1.37) was purified from mycobacterium phlei using (nh4)2so4 precipitation followed by chromatography on sephadex g-200, deae-cellulose on deae sephadex a-50. the purified preparation homogeneous on column chromatography, polyacrylamide gel electrophoresis and sodium dodecyl sulphate gel electrophoresis had a molecular weight of 86 860. the native enzyme was composed of four subunits of equal molecular weight (21 550) and was thermostable ... | 1977 | 922015 |
| [analysis of genes for ribosomal rna in mycobacterium phlei]. | | 1988 | 2452272 |
| induction of cell mediated immune response by nonspecific stimulator of immunity against antigenically unrelated oncogenic virus (marek's disease virus). | an enzyme treated preparation of mycobacterium phlei (nsi), induced strong cell mediated immune response (cmir) against specific as well as against nonspecific oncogenic marek's disease (mdv) in birds, as evinced by lymphocyte migration inhibition, (lmit) lymphocyte transformation test (lt) and lymphokine (lymphocyte migration inhibition factor) lyif assay. maximum cmir could be observed towards third week post inoculation. all the three tests exhibited a positive correlation. such phenomenon of ... | 1989 | 2479599 |
| a study on the receptor for a mycobacteriophage : phage phlei. | from mycobacterium phlei, glycolipid fractions have been isolated which inactivate phage phlei. on the basis of the characteristics of the inactivation (specificity, kinetics, requirement for ca++) typical of the phage-host cell system, it was concluded that these fractions contain the receptor sites for phage phlei ; this conclusion was supported by electron microscopic studies. all the active fractions contain four kinds of components : fatty acids, glycerol, sugars (d-lyxose, 6-0-methyl-d-glu ... | 1976 | 953052 |
| site of action of nonheme iron in the malate (flavine adenine dinucleotide) pathway of mycobacterium phlei. | irradiation with ultraviolet light (360 nm) of cell-free extracts, electron-transport particles, and soluble components from mycobacterium phlei resulted in the loss of malate oxidation by the flavine adenine dinucleotide pathway both in cell-free extracts and reconstituted systems. addition of vitamin k1 restored the loss to the extent of 14% and 11% in cell-free extracts and reconstituted systems respectively. electron-transport particles from m. phlei upon reduction with malate exhibited elec ... | 1976 | 963613 |
| possibilities of the conjugation process in mycobacteria. | results obtained when studying conjugation in mycobacteria by means of different methods are summarized. the method of conjugation on surface of a solid complete medium was tested with different auxotrophic mutants of different strains of mycobacterium smegmatis. it was not possible to obtain positive results even by means of the above method. this was probably due to unsuitability of the chosen strains of mycobacterium smegmatis. preparation of the donor strain by transfer of the f factor from ... | 1975 | 1104423 |
| active transport of glutamine and glutamic acid in membrane vesicles from mycobacterium phlei. | | 1975 | 1125026 |
| different thermostability of l-lactate oxidase from mycobacterium phlei and mycobacterium sp. 279. | | 1975 | 1129380 |
| phospholipids of mycobacterium phlei-1. | | 1975 | 1140310 |
| septic arthritis due to mycobacterium phlei presenting as infantile reiter's syndrome. | we describe a 7-year-old peruvian boy who presented with a 2 week history of conjunctivitis, urethritis and arthritis, in whom mycobacterium phlei was the only organism repeatedly isolated from synovial fluid and tissue and who responded to conventional antituberculous therapy. to our knowledge this is the first documented case of human disease caused by this microorganism. | 1989 | 2810265 |
| immunotherapy of cancer: tumor suppression and regression by cell walls of mycobacterium phlei attached to oil droplets. | components of mycobacterial cell wall(s) (cw) attached to oil droplets were evaluated for their ability 1) to inhibit the growth of line-10 tumor transplants in the skin of syngeneic guinea pigs when inoculated together with 10(6) tumor cells (suppression experiments) and 2) to regress established 7-day-old intradermal tumors and eradicate microscopic lymph node metastases upon injection into the tumors (regression experiments). cw and cell-wall skeleton (cws) preparations from mycobacterium phl ... | 1975 | 1159851 |
| ultrastructure of l-phase variants isolated from a culture of mycobacterium phlei. | relatively stable l-phase colonies were isolated from old cultures of a selected clone of mycobacterium phlei. the colonies grew at 52 degrees c and were composed of rod-shaped, oval or spherical cells. large amoeba-like cells were occasionally present. these were usually limited by a double-layered membrane and devoid of normal cell-wall components such as bacteriophage receptors. the large amoeba-like bodies sometimes showed both outer and inner double-layered membranes, especially in pseudopo ... | 1975 | 1177288 |
| regulation of tricarboxylic acid cycle dehydrogenases in mycobacterium phlei. | | 1975 | 1221033 |
| transfection of "mycobacterium phlei". | | 1975 | 1230056 |
| phenotypic changes in mycobacteria grown in oxygen-limited conditions. | laboratory strains of mycobacterium phlei, m. smegmatis, m. fortuitum, m. gordonae, m. kansasi, m. bovis, m. tuberculosis and m. intracellulare were adapted to grow in an anaerobic environment. concomitant with the transition to anaerobic growth was loss of acid-fastness, loss or modification of colonial pigmentation, and loss of ability to grow on a malachite green-containing medium. the mycobacteria grown anaerobically produced acid from a greater range of carbohydrates than aerobically grown ... | 1986 | 3084791 |