Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the thermal resistance of mycobacterium paratuberculosis in raw milk under conditions simulating pasteurization. | 1993 | 8286469 | |
| association of mycobacterium paratuberculosis infection with reduced mastitis, but with decreased milk production and increased cull rate in clinically normal dairy cows. | approximately 45 holstein cows that were mycobacterium paratuberculosis-positive on the basis of fecal culture results were maintained at any one time in a 210-cow dairy herd. farm management participated in the new york state paratuberculosis eradication program. paratuberculosis-positive cows were grouped separately from paratuberculosis-negative cows, but they were otherwise managed identically. during a 1-year study, 180 paratuberculosis-negative cows and 113 clinically normal paratuberculos ... | 1993 | 8291762 |
| viral association with crohn's disease. | crohn's disease is an inflammatory disorder of the gastrointestinal tract, the cause of which remains unknown. since the first description by dalziel in 1913 (1), similarities between crohn's disease and intestinal mycobacterial infection, particularly johne's disease in ruminants, have been widely recognized (2, 3). after mitchell and rees demonstrated the transmission of granulomata from crohn's disease by injecting intestinal homogenates into the footpads of mice (4), there followed many stud ... | 1993 | 8292306 |
| oral immunization with recombinant bcg induces cellular and humoral immune responses against the foreign antigen. | it has been shown recently that bcg can be used as a live recombinant vaccine to stimulate immune responses. proliferative or cytotoxic t-cell responses against several viral proteins such as hiv gag, env or nef were obtained after parenteral immunization with bcg expressing these proteins. antibody responses were also obtained after immunization of mice with recombinant bcg strain which expressed lac z under the control of a promoter sequence isolated from mycobacterium paratuberculosis. we hav ... | 1993 | 8296480 |
| preparation of a specific rna probe for detection of mycobacterium paratuberculosis and diagnosis of johne's disease. | a species-specific recombinant clone (f57) was obtained from a genomic library of mycobacterium paratuberculosis in the transcription vector pgem 3z. this clone proved to be specific for all mycobacteria tested, including m. avium, and was able to recognize all of the tested m. paratuberculosis strains isolated from animals and humans (patients with crohn's disease). the f57 insert was sequenced and a segment of 620 bp with a g + c content of 58.9% was identified. comparison of the sequence with ... | 1993 | 8315002 |
| immunohistochemical distribution of s-100 alpha-positive cells in bovine mycobacterial and non-mycobacterial granulomas. | by means of immunohistochemistry, the distribution of the alpha-subunit (s-100 alpha) and the beta-subunit (s-100 beta) of s-100 protein was studied in bovine granulomas caused by actinomyces bovis, actinobacillus lignieresi, actinomyces (corynebacterium) pyogenes, pseudomonas aeruginosa, staphylococcus aureus, mycobacterium bovis and mycobacterium paratuberculosis. s-100 alpha-positive epithelioid cells or dendritic cells were scattered among the predominantly s-100 alpha-negative cells of the ... | 1993 | 8315057 |
| inability to detect mycobactin in mycobacteria-infected tissues suggests an alternative iron acquisition mechanism by mycobacteria in vivo. | although most species of mycobacterium are capable of producing mycobactin, it is not known if conditions within the host allow for mycobactin synthesis or whether it even plays a role in iron acquisition in vivo. we employed the mycobactin-auxotroph, mycobacterium paratuberculosis, in a bioassay to examine tissues from animals infected with either mycobacterium tuberculosis, mycobacterium avium or m. paratuberculosis for the presence of mycobactin or compounds which demonstrate mycobactin-like ... | 1993 | 8321124 |
| aetiology of crohn's disease. | 1993 | 8323329 | |
| isolation and sequencing of the gene coding for an antigenic 34-kilodalton protein of mycobacterium paratuberculosis. | the gene coding for an antigenic 34-kda protein of mycobacterium paratuberculosis was isolated and sequenced. the 897-bp open reading frame coded for a novel protein containing specific b epitopes. the occurrence of well-defined hydrophobic and hydrophilic portions suggests the wall location of the protein. | 1993 | 8335649 |
| comparative analysis of three sensitins used in cutaneous testing for tuberculosis and paratuberculosis in cattle. | correspondence between components of sensitins (avian, bovine, and johnin-ppd tuberculins) and the proteins of the corresponding microorganisms (mycobacterium avium, mycobacterium bovis and mycobacterium paratuberculosis) was established. most of these identified proteins are present in the three organisms, but the 38.3- and 48.8-kda proteins of m. avium and the 54.0- and 58.7-kda proteins of m. bovis are endowed with species-specific epitope(s). | 1993 | 8354464 |
| the cellular immunology of bovine paratuberculosis: immunity may be regulated by cd4+ helper and cd8+ immunoregulatory t lymphocytes which down-regulate gamma/delta+ t-cell cytotoxicity. | in a previous investigation, we obtained evidence that the major histocompatibility complex (mhc)-restricted proliferative response of cd4+ lymphocytes to mycobacterium paratuberculosis antigens was depressed in naturally infected and immunized animals. findings suggested that depression of the response was attributable to an abrogation in the ability of cd4+ cells to respond to specific antigens and/or the actual loss of antigen-reactive cells. in vitro cell experiments indicated that the depre ... | 1993 | 8366813 |
| [use of the in vitro enzymatic amplification method for the detection of mycobacterium paratuberculosis in feces]. | a polymerase chain reaction was developed, using as target sequence an insertion element of 1,451 base pairs (is 900), specific for mycobacterium paratuberculosis (15-20 copies per genome). the test was performed in three stages: (1) extraction of bacterial deoxyribonucleic acid (dna), from faeces stored at +4 degrees c, -20 degrees c, in 70% ethanol or in a buffer solution; (2) amplification of the target dna by means of thermostable dna polymerase; (3) detection of the amplified dna by electro ... | 1993 | 8400396 |
| use of an enzyme-linked immunosorbent assay for serodiagnosis of clinical paratuberculosis in goats. study by western blotting of false-positive reactions. | an enzyme-linked immunosorbent assay (elisa) was performed for diagnosis of paratuberculosis in goats, using as antigen a protoplasmic extract (ppa-3). the test was developed on the basis of the results obtained with two serum reference pools, positive and negative respectively. to avoid day-to-day variations, dilutions of the positive serum pool were included in each plate to obtain an arbitrary system, transforming absorbance into immunoglobulin (ig) g anti-mycobacterium paratuberculosis units ... | 1993 | 8400398 |
| antibodies to mycobacterium paratuberculosis. | 1993 | 8406172 | |
| isolation and partial characterization of glycolipid fractions from mycobacterium avium serovar 2 (mycobacterium paratuberculosis 18) that inhibit activated macrophages. | glycolipid fractions from mycobacterium avium serovar 2 (mycobacterium paratuberculosis 18) inhibited the killing of candida albicans by activated bovine peripheral-blood-derived macrophages. fractions were derived by using the matrix solid-phase dispersion technique, which is a new method of simultaneous lysis and partial fractionation of components of bacterial cells. further purification of active fractions was performed by concanavalin a affinity chromatography, centrifugal filtration, and d ... | 1993 | 8418031 |
| causation of crohn's disease: the impact of clusters. | 1993 | 8425709 | |
| serodiagnosis of paratuberculosis in sheep by use of agar gel immunodiffusion. | an agar gel immunodiffusion (agid) test was used over a 3-year period to examine 1,871 serum samples from sheep representing 5 mycobacterium paratuberculosis-infected flocks and 4 flocks presumed to be uninfected. of 1,032 sheep, 31 had positive agid test results (scoring 1 to 5), and 23 of these 31 were necropsied. infection with m paratuberculosis was confirmed by 1 or more of the following findings: observation of typical lesions on histologic examination of sections of ileum or ileocecal lym ... | 1993 | 8427456 |
| cultivation of mycobacterium paratuberculosis from bovine fecal samples by using elements of the roche mb check system. | components of a commercially available, nonradiometric, biphasic (liquid medium then solid medium) system for the detection of mycobacterial species, roche mb check, were adapted for the isolation of mycobacterium paratuberculosis from bovine fecal specimens. a two-stage culture procedure was developed in which processed fecal samples were incubated in modified commercial liquid medium and then subcultured onto herrold's egg yolk medium with mycobactin. by using known culture-positive samples an ... | 1993 | 8432826 |
| relationship between negative skin test with johnin after vaccination and post mortem findings. | one hundred and seventy-six cattle, vaccinated against paratuberculosis in the first month of life with a whole-cell inactivated vaccine, containing mycobacterium paratuberculosis suspended in a water/oil emulsion, were tested with intradermal johnin between the ages of five and 14 months. negative results in the skin test occurred more frequently in animals found positive in post mortem examinations (p = 0.008) using histological, microscopical and cultural techniques. | 1993 | 8442335 |
| paratuberculosis in farmed red deer (cervus elaphus) in ireland. | paratuberculosis was diagnosed in one 18-month-old and two 30-month-old hinds in a herd of 70 red deer (cervus elaphus) in ireland. loss of condition and intermittent diarrhoea were the main clinical findings. clumps of acid-fast organisms were found in the faeces of the three deer. post mortem examination of one deer showed a slight swelling and pallor of the intestinal tract and associated lymph nodes. histopathology showed a severe, granulomatous enteritis and lymphadenitis, with extensive ce ... | 1993 | 8451813 |
| differentiation of mycobacteria on the basis of chemotype profiles by using matrix solid-phase dispersion and thin-layer chromatography. | because of the rising incidence of clinical mycobacterial infections and the difficulty in identification and characterization of mycobacteria at the subspecies and serovar levels, a technique for differentiation that could be performed quickly and with relatively little equipment and expense was developed. lysis and fractionation of mycobacteria by matrix solid-phase dispersion followed by thin-layer chromatography were used to produce chemotype profiles of the lipid and glycolipid components o ... | 1993 | 8458954 |
| reproducibility of a commercial enzyme-linked immunosorbent assay for bovine paratuberculosis among eight laboratories. | interlaboratory reproducibility of an absorbed enzyme-linked immunosorbent assay (elisa) kit for detection of bovine serum antibodies to mycobacterium paratuberculosis was evaluated. a panel of 30 bovine sera (15 positives and 15 negatives) was tested in triplicate microtiter wells on each of 2 days at 8 different laboratories. one laboratory had invalid results because of positive or negative serum control optical density (od) readings beyond the acceptable range specified by the kit. the coeff ... | 1993 | 8466981 |
| antibodies to mycobacterium paratuberculosis and nine species of environmental mycobacteria in crohn's disease and control subjects. | cultural and serological studies have provided limited, often conflicting, evidence of a role for mycobacteria in the pathogenesis of crohn's disease. interest has focussed on mycobacterium paratuberculosis, previously considered to be common in the environment with no major role as a human pathogen. whether a specific serum antibody response to mycobacteria occurs in crohn's disease or ulcerative colitis was investigated. sera from patients with crohn's disease (n = 38), ulcerative colitis (n = ... | 1993 | 8472986 |
| mycobacteria in crohn's disease. | 1993 | 8495187 | |
| identification of spheroplast-like agents isolated from tissues of patients with crohn's disease and control tissues by polymerase chain reaction. | mycobacterium paratuberculosis has been isolated from tissue taken from patients with crohn's disease and has been implicated in the etiology of this disease. on culture, the organisms appear initially as cell wall-deficient, spheroplast-like forms that are difficult to identify by conventional techniques. here we examine 30 unidentified cultures by the polymerase chain reaction using primers specific for m. paratuberculosis, mycobacterium tuberculosis, and mycobacterium avium restriction fragme ... | 1993 | 8501224 |
| effects of administration of anti-cd4 and anti-cd8 monoclonal antibodies on mycobacterium paratuberculosis infection in intragastrically challenged mice. | in this study, we examined the roles of cd4+ and cd8+ cells in resistance to experimental paratuberculosis. mice received purified anti-cd4 or anti-cd8 monoclonal antibodies before intragastric challenge with mycobacterium paratuberculosis and on a biweekly basis for six months. this resulted in sustained depletion of cd4+ and cd8+ cells as verified by flow cytometry analysis of spleen cells from m. paratuberculosis infected mice. depletion of cd4+ or cd8+ cells did not enhance fecal shedding of ... | 1993 | 8509155 |
| [does crohn's disease an infectious origin?]. | 1993 | 8053758 | |
| abdominal lymph-node granulomas and helicobacter pylori. | 1993 | 8101316 | |
| susceptibility of balb/c, c57/b6 and c57/b10 mice to infection with mycobacterium paratuberculosis. | balb/c, c57/b10 and c57/b6 mice were examined for their susceptibility to disseminated mycobacterium paratuberculosis infection after intraperitoneal inoculation with a suspension of organisms containing mineral oil. animals were examined monthly by histopathology and bacterial tissue counts of liver and spleen over a 6-month period. only balb/c mice maintained a steady infection with an average of 4.1 x 10(5) +/- 7.8 x 10(3) and 8.1 x 10(5) +/- 2.6 x 10(4) colony forming units (cfu) per gram of ... | 1993 | 8106664 |
| detection of mycobacterium paratuberculosis by polymerase chain reaction in children with crohn's disease. | mycobacterium paratuberculosis, the causative agent of johne's disease (a chronic enteritis in ruminants), has been suspected to be involved in crohn's disease. in this study, polymerase chain reaction was used to detect the presence of is900 dna sequences specific to m. paratuberculosis genomes in biopsies and surgical resections from 53 children with various gastrointestinal diseases and disorders. is900 sequences were found in 13 of 18 samples from patients with crohn's disease (72%; p < .01 ... | 1994 | 8106782 |
| histopathological and morphometrical comparison of granulomatous lesions in balb/c and c3h/hej mice inoculated with mycobacterium paratuberculosis. | susceptibility to mycobacterium paratuberculosis strain atcc 19698 infection was compared histologically and morphometrically in balb/c (bcgs) and c3h/hej (bcgr) mice. the granulomatous lesions, containing m. paratuberculosis, in the liver and spleen of balb/c mice increased progressively until 32 weeks after intraperitoneal inoculation. in contrast, both the granulomatous lesions and number of bacteria decreased drastically in c3h/hej mice beginning at 6 weeks after inoculation. there were hist ... | 1994 | 8056872 |
| mycobacterium paratuberculosis infection in two llamas. | mycobacterium paratuberculosis was diagnosed in 2 llamas by bacteriologic culturing and dna probe testing of fecal and intestinal specimens. diarrhea and weight loss were observed in both animals. lesions in the gastrointestinal tracts of the 2 llamas were similar to pathologic changes reported in cattle infected with m paratuberculosis. | 1994 | 8063605 |
| comparison of immunohistochemistry, acid-fast staining, and cultivation for detection of mycobacterium paratuberculosis in goats. | forty-seven paired specimens of ileum and mesenterial lymph nodes from goats originating from 2 herds with paratuberculosis were investigated. culture of the specimens for mycobacterium paratuberculosis was compared with ziehl-neelsen staining and with immunohistochemical tests on paraffin-embedded tissue sections, using a m. paratuberculosis immune serum and an avidin-biotin-alkaline phosphatase method. immunohistochemical techniques detected most positive samples and produced more clearly visi ... | 1994 | 8068751 |
| sample preparation for m paratuberculosis polymerase chain reaction in faeces and tissues. | 1994 | 8737487 | |
| [a farm with paratuberculosis]. | 1994 | 8134912 | |
| herd prevalence and geographic distribution of, and risk factors for, bovine paratuberculosis in wisconsin. | a random sample of wisconsin dairy herds, stratified by herd size, were tested for paratuberculosis by use of an absorbed elisa procedure. the elisa was optimized for overall accuracy by means of receiver operating characteristic curve analysis, and had a sensitivity and specificity of 50.9 and 94.9%, respectively. herd prevalence was analyzed for correlation with responses to a management practices questionnaire completed by the herd owners. one hundred and fifty-eight herds and 4,990 cattle we ... | 1994 | 8163422 |
| specific detection of mycobacterium paratuberculosis dna associated with granulomatous tissue in crohn's disease. | the role of mycobacteria, specifically mycobacterium paratuberculosis, in crohn's disease has aroused considerable controversy for many years. using the ultra sensitive polymerase chain reaction some studies have reported detection of m paratuberculosis dna in as many as 65% of crohn's disease patients but also in patients without disease. other studies have been negative for both groups. we therefore designed a double blind control trial to investigate the presence of mycobacterial dna in age, ... | 1994 | 8174989 |
| a rapid method for the extraction and detection of mycobacterium avium subspecies paratuberculosis from clinical specimens. | 1994 | 8178419 | |
| in vivo administration of a monoclonal antibody against the type i il-1 receptor inhibits the ability of mice to eliminate mycobacterium paratuberculosis. | the purpose of this study was to determine the influence of endogenous interleukin-1 (il-1) on resistance to paratuberculosis infection in experimentally infected gnotobiotic mice. following a 6-month treatment with prednisolone to facilitate bacillary multiplication, control mice substantially reduced the numbers of m. paratuberculosis in the liver and ileum. in contrast, mice injected with a monoclonal antibody against the type i il-1 receptor failed to reduce the numbers of m. paratuberculosi ... | 1994 | 8195697 |
| mycobacteria in the human intestine. | 1994 | 8200578 | |
| [value of serological determinations in the differential diagnosis of ulcerative colitis and crohn's disease, and immunogenetic aspects]. | 1994 | 7800073 | |
| comparison of a complement fixation test, a gel diffusion test and two absorbed and unabsorbed elisas for the diagnosis of paratuberculosis in sheep. | a complement fixation test for paratuberculosis, a gel diffusion test and two enzyme-linked immunosorbent assays (elisa) were evaluated using sera from mycobacterium paratuberculosis infected and non-infected sheep. gross pathology and histopathology were used as parameters of infection. the two elisas, one of which is commercially available for testing cattle, were used before and after sera had been absorbed with a soluble sonicate of mycobacterium phlei. differences between the various tests ... | 1994 | 7801513 |
| the effect of vaccination on the prevalence of paratuberculosis in large dairy herds. | eight hundred and sixty-six one-month-old female calves were vaccinated using heat-killed mycobacterium paratuberculosis in oil during a five-year period. seven hundred and twenty-one bull calves and 379 female calves served as nonvaccinated controls. two large herds were used in the study. the calves were tested by faecal culture and by serologic tests on the day of vaccination, at 3, 6, 9 and 12 months of age, at breeding and on the day of parturition. the results were evaluated by trend analy ... | 1994 | 7801515 |
| nucleotide sequence and secondary structures of precursor 16s rrna of slow-growing mycobacteria. | slow-growing mycobacteria have a single ribosomal rna (rrn) operon, with the genes for 16s, 23s and 5s rrna being present in that order. the transcription start site of the rrn operon of mycobacterium tuberculosis was identified in escherichia coli. pcr methodology was used to amplify parts of the rrn operon, namely the leader region and the spacer-1 region separating the 16s rrna and 23s rrna genes of mycobacterium avium, mycobacterium paratuberculosis, mycobacterium intracellulare, 'mycobacter ... | 1994 | 7512868 |
| mycobacterium paratuberculosis and escherichia coli share common antigenic determinants. | paratuberculosis (johne's disease) is a chronic enteritis of ruminants, which is due to infection by mycobacterium paratuberculosis. by comparative analysis of western blots of m. paratuberculosis components incubated with sera from paratuberculous cows (either pre-absorbed or not on an escherichia coli sonicate), we have shown the presence of cross-reactive antigens between m. paratuberculosis and e. coli. components in the range of 22 to 75 kda are recognized by sera from paratuberculous cows, ... | 1994 | 7518988 |
| identification and characterization of mycobacterium paratuberculosis recombinant proteins expressed in e. coli. | mycobacterium paratuberculosis is the causative agent of johne's disease, a chronic enteritis in ruminants, and it has also been isolated and identified from patients with crohn's disease, an inflammatory bowel disease. the control of johne's disease has been hampered by the lack of a reliable diagnostic test because of the large degree of antigenic cross-reactivity between mycobacterial and non-mycobacterial species. to help identify specific antigen(s) or epitope(s), an m. paratuberculosis exp ... | 1994 | 7765093 |
| mycobacterial cell wall components induce the production of tnf-alpha, il-1, and il-6 by bovine monocytes and the murine macrophage cell line raw 264.7. | johne's disease is characterized by a chronic enteritis that results in granulomatous inflammation, cachexia, and eventual death of cattle infected with mycobacterium paratuberculosis. the cytokines tumor necrosis factor-alpha (tnf-alpha), interleukin-1 (il-1), and interleukin-6 (il-6) have been associated with granuloma formation and wasting in other disease syndromes. the potential role of these cytokines in the development and progression of johne's disease has not been investigated. freshly ... | 1994 | 7830527 |
| putative functional domain within orf2 on the mycobacterium insertion sequences is900 and is902. | repeated dna sequences have been identified in a range of mycobacterial species and have been implicated in the increased virulence of some of these species, namely, mycobacterium paratuberculosis and m. avium subsp. silvaticum. here we present a case to suggest that the insertion sequences is900 and is902 encode a protein from a putative gene positioned on the complementary strand to their transposase genes. based on amino acid homology analyses, this open reading frame (orf2) could encode a tr ... | 1994 | 7835987 |
| mycobacterium paratuberculosis in intestinal tissue from patients with crohn's disease demonstrated by a nested primer polymerase chain reaction. | the etiology of crohn's disease remains unknown, but current research has concentrated on autoimmunity and/or mycobacterial infection. the polymerase chain reaction (pcr) enables the detection of genetic material even when very few microorganisms are present. | 1994 | 7839099 |
| cloning and expression in escherichia coli of dna encoding a 60 kda stress protein of mycobacterium paratuberculosis, the causative agent of johne's disease. | polymerase chain reaction (pcr) was used to generate dna encoding a 60 kda stress protein of mycobacterium paratuberculosis using primers complementary to sequences at the 5' and 3' ends of 60 kda stress protein genes (encoding the '65 kda antigens') of m. leprae and m. tuberculosis. the predicted pcr product of 1.8 kb contained the entire coding sequence of an m. paratuberculosis 60 kda stress protein, with non-coding regions of 124 bp and 1 bp at the 5' and 3' ends, respectively. dna encoding ... | 1994 | 7881551 |
| use of indicators for monitoring the reduction of pathogens in animal waste treated in biogas plants. | the possibility of including bacterial indicators in programmes for monitoring the disinfection of animal waste treated in biogas plants was examined. five full-scale plants receiving waste from several farms were monitored. of these, two used thermophilic and one mesophilic digestion exclusively whereas two employed a two stage process involving thermophilic pretreatment followed by mesophilic digestion. for each plant the reducing effect on bacterial pathogens present in the incoming waste and ... | 1994 | 7916882 |
| identification and characterization of a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis. | a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. the gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kda in m. a. paratuberculosis. the clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative se ... | 1994 | 7921248 |
| paratuberculosis. | paratuberculosis (johne's disease) is a chronic, wasting, widespread mycobacteriosis of ruminants. it involves extensive mycobacterial shedding, which accounts for the high contagiousness, and ends with a fatal enteritis. decreases in weight, milk production, and fertility produce severe economic loss. the dna of the etiological agent (mycobacterium paratuberculosis) has a base composition (66 to 67% g+c) within the range of that of mycobacteria (62 to 70% g+c), a size (4.4 x 10(6) to 4.7 x 10(6 ... | 1994 | 7923053 |
| effect of combination therapy in mycobacterium paratuberculosis infected rabbits. | rabbits, infected with m paratuberculosis of caprine origin, were treated with streptomycin sulphate and rifampicin in combination with levamisole hydrochloride for a period of 2 months. marked clinical response including significant rise in total serum protein, albumin and globulin were recorded in treated groups. highly significant higher leucocyte migration inhibition indicating enhanced cmi reaction, occurred in rabbits treated for 4 weeks. complete elimination of m. paratuberculosis from fa ... | 1994 | 7927524 |
| [occurrence, economic significance and diagnosis of paratuberculosis]. | paratuberculosis is the most serious chronic bacterial disease of ruminants. regarding the increasing incidence of the disease in the czech republic in connection with changing situation in agriculture and, among other factors, with import of animals we are presenting a summary of the latest data. we assume that high economic losses and expected participation of mycobacterium paratuberculosis in crohn's disease in men will evoke a broad interest in the disease among people from various branches ... | 1994 | 7941227 |
| distribution and hybridization patterns of the insertion element is900 in clinical isolates of mycobacterium paratuberculosis. | reference strains and 31 clinical isolates of m. paratuberculosis, mainly from goats, were analysed for restriction fragment length polymorphism (rflp). restriction digests of bacterial dna were hybridized with a repetitive insertion sequence, is900, to obtain banding patterns for comparison of strains. twenty-five of the 31 field-strains hybridized with is900, and five hybridization patterns were identified. it was not possible to identify specific patterns for goat strains of m. paratuberculos ... | 1994 | 7941294 |
| diagnosis of paratuberculosis in dairy cattle, using enzyme-linked immunosorbent assay for detection of antibodies against mycobacterium paratuberculosis in milk. | an elisa containing lipoarabinomannan (lam) antigen was used to detect antibodies in milk and serum for diagnosis of mycobacterium paratuberculosis infection in dairy cattle. in experiment 1, milk and serum samples were obtained from 25 cows, and subjected to lam elisa testing immediately, and after 1 year of storage at -70 c. milk samples, with and without a commonly used chemical preservative, were tested. there was no significant difference in lam elisa results between fresh and frozen sample ... | 1994 | 7978627 |
| [humoral response to mycobacteria in patients with crohn disease]. | the recent recovery of mycobacterium paratuberculosis from tissues of patients with crohn's disease has highlighted the possible etiologic role of this microorganism in the disease. however, the immunological evidence generated by various groups supporting this hypothesis is as yet inconclusive. a specific antibody response might be masked in these patients by the wide antigenic homologies prevailing within the genus mycobacterium. the present study was undertaken with the purpose of exploring t ... | 1994 | 7997145 |
| comparison of cellular and extracellular proteins expressed by various isolates of mycobacterium paratuberculosis and other mycobacterial species. | protein expression profiles of 10 isolates of mycobacterium paratuberculosis, m avium 18 (formerly m paratuberculosis 18), and 1 isolate each of m avium serotype 2, m avium serotype 8, and m bovis bcg were examined. protein expression profiles of m paratuberculosis and m avium were similar. however, two-dimensional gel analysis of [35s]methionine-labeled cellular proteins resolved 4 proteins, with molecular mass of 28,000, 32,000, 32,000, and 42,000 daltons, which were expressed in greater amoun ... | 1994 | 7998697 |
| purification, characterization, gene sequence, and significance of a bacterioferritin from mycobacterium leprae. | the study of tissue-derived mycobacterium leprae provides insights to the immunopathology of leprosy and helps identify broad molecular features necessary for mycobacterial parasitism. a major membrane protein (mmp-ii) of in vivo-derived m. leprae previously recognized (hunter, s.w., b. rivoire, v. mehra, b.r. bloom, and p.j. brennan. 1990. j. biol. chem. 265:14065) was purified from extracts of the organism and partial amino acid sequence obtained. this information allowed recognition, within o ... | 1994 | 8006590 |
| evaluation of an enzyme-linked immunosorbent assay for diagnosis of paratuberculosis in goats. | a commercial rapid-absorbed elisa developed to detect antibodies to mycobacterium paratuberculosis in bovine serum was modified for use with goat serum. diagnostic sensitivity was evaluated, using a group of 163 goats from a herd with endemic paratuberculosis. blood and fecal samples were obtained simultaneously, and prevalence of shedding of m paratuberculosis in the feces was estimated by detection of dna of the mycobacterial insertion sequence, is900, using a commercial test kit. diagnostic s ... | 1994 | 8017689 |
| comparison of the 23s ribosomal rna genes and the spacer region between the 16s and 23s rrna genes of the closely related mycobacterium avium and mycobacterium paratuberculosis and the fast-growing mycobacterium phlei. | the 23s rdna sequences of mycobacterium paratuberculosis, m. avium and m. phlei and the sequences of the spacer regions between the 16s and 23s rrna genes were determined. the overall 23s rdna sequence identity between m. paratuberculosis and m. avium was 99.7% (nine mismatches), showing the very close relatedness of these mycobacteria. evolutionary distances between the five known mycobacterial 23s rrna/rdna sequences and those of other gram-positive g + c-rich bacteria were determined. the 23s ... | 1994 | 8025676 |
| experimental infection of vaccinated and non-vaccinated lambs with mycobacterium paratuberculosis. | a study of the pathogenesis of paratuberculosis and the effects of vaccination was carried out on 17 rasa lambs, allocated to four groups. vaccination seemed to accelerate the progress of the infection, and led quickly to healing. the only site of early lesions was the interfollicular areas in the peyer's patches. this would suggest an explanation both for the location of paratuberculous lesions in the ileum in clinical cases, and for the lower susceptibility to infection of adult animals, in wh ... | 1994 | 8040384 |
| the role of mycobacteria in crohn's disease. | 1994 | 8046739 | |
| development of species-specific enzyme-linked immunosorbent assay for diagnosis of johne's disease in cattle. | the previously described (m. de kesel, p. gilot, m.-c. misonne, m. coene, and c. cocito, j. clin. microbiol., 31:947-954, 1993) a362 recombinant polypeptide of mycobacterium paratuberculosis was used as reagent for an enzyme-linked immunosorbent assay (elisa). this elisa, which is endowed with species specificity with respect to the other mycobacteria, was applied to the analysis of bovine paratuberculosis (johne's disease), an endemic mycobacteriosis of cattle caused by m. paratuberculosis. the ... | 1994 | 8051246 |
| characterization of is1110, a highly mobile genetic element from mycobacterium avium. | a highly mobile insertion sequence designated is1110 was detected in mycobacterium avium strain lr541 following an observed increase in size of the plasmid plr20. genomic libraries of m. avium strains carrying either parental plr20 or the modified plasmid (plr20') were constructed and the sequence of the relevant clones was determined to characterize the insertion sequence and the target region. is1110 is a 1457 bp element lacking terminal inverted repeats, and is related to is900 (from mycobact ... | 1994 | 8052124 |
| distribution of anti-cd68 (ebm11) immunoreactivity in formalin-fixed, paraffin-embedded bovine tissues. | a commercially acquired anti-human macrophage antibody (anti-cd68; ebm11) was used in an immunocytochemical technique to detect macrophages in formalin-fixed, paraffin-embedded tissues from cattle, pigs, humans, rats, turkeys, dogs, and cats. in healthy cattle, the antibody labeled alveolar macrophages, pulmonary intravascular cells (presumably intravascular macrophages), and macrophage-like cells in other tissues. in bovine lungs infected with pasteurella haemolytica, ebm11 antibody labeled 95% ... | 1994 | 8053129 |
| detection of pcr products from mycobacterium avium subspecies paratuberculosis using oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups. | a pool of five oligonucleotides has been used to detect the pathogenic organism mycobacterium avium subspecies paratuberculosis in pcr-amplified dna from ruminants. the oligonucleotides were labelled at the 5'-end with three dinitrophenyl reporter groups and hybridised to the target dna, which was fixed to a nylon membrane by ultraviolet irradiation. colourimetric detection of the pcr product was carried out using an anti-dnp antibody conjugated to horseradish peroxidase or to alkaline phosphata ... | 1994 | 9346864 |
| incidence of paratuberculosis after vaccination against m. paratuberculosis in two infected dairy herds. | vaccination against paratuberculosis of all newborn animals has been performed since april 1984 in two dairy herds with a high incidence of clinical cases of paratuberculosis, using a vaccine containing heat-inactivated m. paratuberculosis in a water/mineral oil emulsion. animals slaughtered between april 1984 and january 1991 were included in the study. histology, bacterioscopy and culture on smith and modified löwenstein-jensen media were performed using jejunum, ileum and draining lymph nodes ... | 1994 | 7701865 |
| specificity of the immunohistochemical demonstration of mycobacterial antigens. | the specificity of immunohistological reactions of commercial polyclonal antibodies to antigens of various mycobacteria (mycobacterium bovis, mycobacterium duvalii and mycobacterium paratuberculosis) was tested in connection with a number of pathogens and with the help of the indirect immunoperoxidase technique. positive reactions occurred with various mycobacterial antigens and some gram-positive bacteria and fungi. these investigations indicate that when the above antibodies are used in histop ... | 1994 | 7701868 |
| occurrence, in crohn's disease, of antibodies directed against a species-specific recombinant polypeptide of mycobacterium paratuberculosis. | sera from patients with crohn's disease and control were analyzed by an enzyme-linked immunosorbent assay based on the mycobacterium paratuberculosis-specific recombinant polypeptide a362. anti-a362 immunoglobulin g (igg) (p < 0.05) and iga (p < 0.001) titers were higher in patients with crohn's disease than in controls. a monomodal gaussian distribution of anti-a362 iga levels were found for controls, and a bimodal distribution was found for patients with crohn's disease. an m. paratuberculosis ... | 1994 | 7496954 |
| the role of restricted food intake in the pathogenesis of cachexia in severe combined immunodeficient beige mice infected with mycobacterium paratuberculosis. | a paired feeding experiment was conducted to investigate if reduced food intake is a reason for the body weight loss previously observed in severe combined immunodeficient beige (scid bg) mice infected with mycobacterium paratuberculosis. mice were paired on the basis of age, litter and sex. one of each pair was injected intraperitoneally with 10(5) viable m. paratuberculosis organisms. the remainder served as uninfected pairfed mates. each uninfected mouse was restricted to the amount of food ( ... | 1995 | 7704841 |
| [the prevalence of paratuberculosis in the plateau de diesse region]. | we analysed 595 cows in 20 herds for serological evidence of paratuberculosis with an elisa procedure for which we had recently determined a sensitivity of 50% and a specificity of 98%, and we obtained an overall calculated real prevalence of 5.99%. in two herds with clinical cases of paratuberculosis, the calculated true prevalence of seroreactors was 21.47%. in 9 herds with clinical suspicion of johne's disease, the rate was 4.23%, 9 herds without any clinical suspicion showed a mean prevalenc ... | 1995 | 7716481 |
| characterization by restriction endonuclease analysis and dna hybridization using is900 of bovine, ovine, caprine and human dependent strains of mycobacterium paratuberculosis isolated in various localities. | dna of 90 mycobactin-dependent strains of mycobacterium paratuberculosis, isolated in 9 countries, was digested with restriction endonuclease psti and hybridized with a dna fragment containing insertion sequence is900. bovine strains (n = 73) were isolated from 61 animals in 17 herds, ovine strains (n = 15) from 13 animals in 3 herds and the set was completed by 1 caprine and 1 human (linda) strain. three types, tentatively designated a (n = 37), b (n = 51) and c (n = 2) were differentiated by r ... | 1995 | 7483244 |
| histopathology of c57bl/6 mice inoculated orally with mycobacterium paratuberculosis. | the susceptibility of c57bl/6 mice to oral inoculation with myobacterium paratuberculosis was evaluated histopathologically. granulomatous lesions containing acid-fast bacteria developed in the mesenteric lymph nodes in over 50% of the mice by 11 months after inoculation. the results suggest that c57bl/6 mice may be useful for studying infection, pathogenesis, and other aspects of paratuberculosis. | 1995 | 7490340 |
| recombinant bcg strains expressing the sivmac251nef gene induce proliferative and ctl responses against nef synthetic peptides in mice. | ctl responses are known to be important for the control of hiv and siv infections. such responses are targeted against various components of these viruses including regulatory proteins like nef. the sivmac251nef gene was cloned in mycobacterium bovis bcg under the control of p(an), a promoter from mycobacterium paratuberculosis. nef was expressed as a fused polypeptide with orf2, an open reading frame adjacent to p(an). mice inoculated with rbcg(sivmac251nef) exhibited proliferative and cd8+ cyt ... | 1995 | 7543715 |
| detection of specific t cell reactivity in sheep infected with mycobacterium avium subspecies silvaticum and paratuberculosis using two defined mycobacterial antigens. | a 30 kda antigen (p30) from mycobacterium avium ssp. paratuberculosis (m. a. paratuberculosis) and a 40 kda (p40) antigen from mycobacterium avium ssp. silvaticum (m. a. silvaticum) were employed in two different assays to measure the cell-mediated immune reactivity of ovine peripheral blood lymphocytes. in lymphocyte stimulation assays, proliferative responses to the p30 were observed only with lymphocytes from sheep inoculated with live m. a. paratuberculosis or m. a. silvaticum. although this ... | 1995 | 7545852 |
| recombinant bcg expressing the leishmania surface antigen gp63 induces protective immunity against leishmania major infection in balb/c mice. | we have cloned and expressed the gp63 gene of leishmania major in bcg to develop a recombinant vaccine against zoonotic cutaneous leishmaniasis. two different expression systems were investigated. the first system consists of pan, a mycobacterium paratuberculosis promoter, which drives expression of orf2, an open reading frame in is900. this system allows the production of heterologous polypeptides as hybrids with the orf2 gene product. the second expression system relies on the production of an ... | 1995 | 7551026 |
| novel glycolipids of mycobacterium avium and related m. paratuberculosis strains of relevance to aids and crohn's disease. | the polar glycolipid fractions of several mycobacterial strains of the closely related species m. avium and m. paratuberculosis have been analysed by thin layer chromatography (tlc), high ph anion exchange chromatography (hpaec), gas-liquid chromatography (gc) and nuclear magnetic resonance (nmr) spectroscopy. the upper phase of a folch partitioning (rather than the lower phase analysed by others) was subjected to tlc in solvent system chloroform-methanol-water 50:40:10 v/v/v. a major band was p ... | 1995 | 7554817 |
| lack of mycobactin dependence of mycobacteria isolated on middlebrook 7h11 from clinical cases of ovine paratuberculosis. | mycobacterium avium subsp. paratuberculosis is distinguished from other mycobacterial species by its dependence on the iron-chelator factor mycobactin and its prolonged incubation period when grown in vitro. traditionally, a very low rate of isolation has been considered characteristic of sheep strains of this mycobacterium species. in the present study, a comparison was made of the performance of two media, löwenstein-jensen and middlebrook 7h11/oadc both with and without mycobactin, on the iso ... | 1995 | 7571372 |
| evaluation of an enzyme-linked immunosorbent assay licensed by the usda for use in cattle for diagnosis of ovine paratuberculosis. | a commercially available mycobacterium phlei-absorbed enzyme-linked immunosorbent assay (elisa) approved to detect antibodies to mycobacterium paratuberculosis in cattle was evaluated for its applicability in sheep. the potential for interference with elisa results from cross-reacting antibodies to corynebacterium pseudotuberculosis was also investigated. serum samples were randomly selected from a collection of samples obtained in 1986-1991 from 6 infected and 5 noninfected sheep flocks varying ... | 1995 | 7578450 |
| immunoaffinity chromatographic isolation of a high molecular weight seroreactive protein from mycobacterium leprae cell sonicate. | the purpose of this study was to isolate mycobacterium leprae antigen(s) by immunoaffinity chromatography using immunoglobulins from leprosy patients and from rabbit anti-m. leprae hyperimmune serum coupled to cnbr-sepharose 4b. a high molecular weight (m(r)) m. leprae protein (mlp) with a subunit m(r) of 22,000 was isolated. mlp was recognized by monoclonal antibody mmpii1g4 which is known to react with mmpii, a 22 kda protein of m. leprae. the n-terminal sequence of the 22 kda subunit (met-gln ... | 1995 | 7581267 |
| effect of intestinal resection on serum antibodies to the mycobacterial 45/48 kilodalton doublet antigen in crohn's disease. | interest in the role of mycobacterial infection in crohn's disease has been revived by the cultural detection of mycobacterium paratuberculosis in patients with crohn's disease. this hypothesis was examined serologically using assays with high specificity for crohn's disease. the effect of intestinal resection on serum antibodies specific for crohn's disease was investigated with an immunoblot assay and an enzyme linked immunosorbent assay using the 45/48 kilodalton doublet antigen of mycobacter ... | 1995 | 7590431 |
| glycosylation of igg during potentially arthritogenic lentiviral infections. | agalactosyl igg [gal(0)] was first discovered in patients with rheumatoid arthritis (ra). however, the proportion of this glycoform is also raised in tuberculosis and leprosy. this has helped reinforce the suggestion that ra may be triggered by a mycobacterium-like slow bacterial infection. on the other hand, arthritis can occur in mycobacterial diseases, so raised gal(0) could be associated with a tendency to arthritis, rather than with a particular type of infection. therefore, we wished to fi ... | 1995 | 7597380 |
| diagnosis of infection with mycobacterium paratuberculosis. | 1995 | 7600511 | |
| johne's disease in red deer. | 1995 | 7604515 | |
| pathogenesis of crohn's disease: the environment revisited. | 1995 | 7614097 | |
| mycobacterium paratuberculosis and crohn's disease. | 1995 | 7615290 | |
| johne's disease in alpacas (lama pacos) in australia. | johne's disease was diagnosed in 10 alpacas (lama pacos) in australia between february 1993 and may 1994. eight of the animals were between 12 and 24 months of age, one was a 6-year-old female, and one was a 4-year-old male. five, including the 6-year-old and the 4-year-old alpacas, showed weight loss and diarrhoea before death or slaughter. the other cases showed no clinical signs of johne's disease but 4 gave a positive result on faecal culture and one gave a positive result on testing with th ... | 1995 | 7646382 |
| pathogenesis of crohn's disease. | 1995 | 7648238 | |
| johne's disease in a herd of farmed red deer. | an outbreak of johne's disease in a herd of farmed red deer was studied for four years. serological, histopathological and cultural techniques were used to monitor the progress of the disease, and delayed type hypersensitivity skin tests were also applied. the results of the serological tests showed that they were poor predictors of future clinical cases and did not consistently identify animals harbouring mycobacteria. the histopathological methods provided a sensitive and specific means of con ... | 1995 | 7661952 |
| immortalization and characterization of bovine peritoneal macrophages transfected with sv40 plasmid dna. | a transformed bovine peritoneal macrophage cell line was developed and characterized. primary peritoneal macrophages were transformed by calcium-phosphate transfection with sv40 plasmid dna. the transformed cell line retained the morphology of resident peritoneal macrophages as determined by light microscopy and histochemical analysis showed non-specific esterase activity. in addition, immunohistochemical staining of transformed peritoneal macrophages for lysozyme activity was positive. transfor ... | 1995 | 7676607 |
| temporal effects of tumor necrosis factor-alpha on intracellular survival of mycobacterium paratuberculosis. | the causative agent in johne's disease is mycobacterium paratuberculosis, an intracellular pathogen which causes enteritis in ruminants. little is known about interactions between the host cell (macrophage) and m. paratuberculosis; however, this bacterium is able to evade normal host immune defenses and cause a chronic infective state. in the present study, we evaluated whether activation of a murine macrophage cell line (j774.16) by pretreatment with recombinant murine tumor necrosis factor alp ... | 1995 | 7676613 |
| phage infection, transfection and transformation of mycobacterium avium complex and mycobacterium paratuberculosis. | mycobacterium avium complex strains and mycobacterium paratuberculosis are closely related intracellular pathogens affecting humans and animals. m. avium complex infections are a leading cause of morbidity and mortality in aids patients, and m. paratuberculosis is the agent of johne's disease in ruminants. genetic manipulation of these micro-organisms would facilitate the understanding of their pathogenesis, the construction of attenuated vaccine strains and the development of new drugs and trea ... | 1995 | 7773411 |
| solid-phase hybridization capture of low-abundance target dna sequences: application to the polymerase chain reaction detection of mycobacterium paratuberculosis and mycobacterium avium subsp. silvaticum. | polymerase chain reaction (pcr) has been widely applied to the detection of microorganisms. overall sensitivity of pcr tests may be substantially reduced due to a large excess of nontarget dna and inhibitory substances in the sample. we used a 5'-biotinylated 513-bp probe from the 3' region of the is 900 element specific for mycobacterium paratuberculosis (mptb) to capture target mptb dna from crude sample dna extracts. captured target dna was separated using streptavidin-coated magnetic particl ... | 1995 | 7793635 |
| mycobacteria in the intestine of japanese patients with inflammatory bowel disease. | it is still controversial whether or not a mycobacterial infection may be a cause of crohn's disease. mycobacterium paratuberculosis may be very difficult to detect using routine culture techniques. to clarify this, we detected mycobacterial dna in patients with inflammatory bowel disease. | 1995 | 7801954 |
| mycobacterium paratuberculosis dna not detected in crohn's disease tissue by fluorescent polymerase chain reaction. | the role of mycobacteria in the aetiology of crohn's disease has been a contentious subject for many years. mycobacterium paratuberculosis is known to cause a chronic granulomatous enteritis in animals (johne's disease) and has been implicated as a possible infectious cause of crohn's disease. however this fastidious organism is only rarely detected by conventional microbiological techniques. this study used oligonucleotide primers to the species-specific m paratuberculosis is900 dna insertion e ... | 1995 | 8549942 |
| ex vivo induction of tnf-alpha and il-6 mrna in bovine whole blood by mycobacterium paratuberculosis and mycobacterial cell wall components. | johne's disease is a chronic enteritis of cattle and other ruminant species that is of worldwide economic importance. the cytokines tumor necrosis factor-alpha (tnf-alpha) and interleukin-6 (il-6) have been associated with granuloma formation and wasting in other disease syndromes. the potential role of these cytokines in the development and progression of johne's disease has not been investigated. using reverse transcriptase polymerase chain reaction (rt-pcr) and specific bovine oligonucleotide ... | 1995 | 8559037 |
| nucleotide sequence analysis and seroreactivities of the 65k heat shock protein from mycobacterium paratuberculosis. | mycobacterium paratuberculosis is the causative agent of johne's disease, a chronic enteritis in ruminants. it has also been implicated as a possible cause of crohn's disease, an inflammatory bowel disease of unknown etiology. the mycobacterial 65k heat shock proteins (hsp-65k) are among the most extensively studied mycobacterial proteins, and their immunogenic characteristics have been suggested to be the basis for autoimmunization in chronic inflammatory diseases. in this context, we isolated ... | 1995 | 8574825 |
| mycobacteria on trial: guilty or innocent in the pathogenesis of crohn's disease? | 1995 | 8789307 |