Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| comparative bioactivation of the novel anti-tuberculosis agent pa-824 in mycobacteria and a subcellular fraction of human liver. | pa-824 is a 2-nitroimidazooxazine prodrug currently in phase ii clinical trial for tuberculosis therapy. it is bioactivated by a deazaflavin (f(420) )-dependent nitroreductase (ddn) isolated from mycobacterium tuberculosis to form a des-nitro metabolite. this releases toxic reactive nitrogen species which may be responsible for its anti-mycobacterial activity. there are no published reports of mammalian enzymes bioactivating this prodrug. we have investigated the metabolism of pa-824 following i ... | 2011 | 20955364 |
| temporal changes in sphingomonas and mycobacterium populations in mangrove sediments contaminated with different concentrations of polycyclic aromatic hydrocarbons (pahs). | the change in community diversity and structure of the indigenous, dominant, polycyclic aromatic hydrocarbon (pah)-degrading bacterial genera, sphingomonas and mycobacterium, due to contamination in the environment is not very well known. a combination of pcr-dgge with specific primers and a cultivation-dependent microbiological method was used to detect different populations of sphingomonas and mycobacterium in mangrove sediments. the structure of the entire bacterial community (including sphin ... | 2011 | 20926106 |
| optimization of inhaled therapies for tuberculosis: the role of macrophages and dendritic cells. | inhaled therapies in the form of drugs or vaccines for tuberculosis treatment were reported about a decade ago. experts around the world met to discuss the scientific progress in inhaled therapies at the international symposium "optimization of inhaled tuberculosis therapies and implications for host-pathogen interactions" held in new delhi, india on november 3-5, 2009. the meeting was organized by the central drug research institute (cdri) lucknow, india. the lung is the main route for infectio ... | 2011 | 20888298 |
| mycobacterial diseases in pediatric hematopoietic sct recipients. | 2011 | 20697370 | |
| structure-based in-silico rational design of a selective peptide inhibitor for thymidine monophosphate kinase of mycobacterium tuberculosis. | tuberculosis still remains one of the most deadly infectious diseases. the emergence of drug resistant strains has fuelled the quest for novel drugs and drug targets for its successful treatment. thymidine monophosphate kinase (tmpk) lies at the point where the salvage and de novo synthetic pathways meet in nucleotide synthesis. tmpk in m.tb has emerged as an attractive drug target since blocking it will affect both the pathways involved in the thymidine triphosphate synthesis. moreover, the uni ... | 2011 | 20697760 |
| lichen scrofulosorum mimicking lichen planus. | lichen scrofulosorum is the most uncommon clinicopathologic variant of the tuberculids. usually, the eruption appears in children and adolescents with strong immune sensitivity to mycobacterium tuberculosis and consists of tiny follicular papules, closely resembling lichen nitidus. we report a case of lichen scrofulosorum in an adult male with active cervical scrofuloderma who developed lesions of lichen scrofulosorum mimicking clinically lichen planus. histopathologic study demonstrated granulo ... | 2011 | 20711063 |
| biodegradation of pyrene in sand, silt and clay fractions of sediment. | microbial degradation is the dominant pathway for natural attenuation of pahs in environmental compartments such as sediments, which in turn depends on the bioavailability of pahs. the bioavailability of pahs has seldom been studied at the sediment particle size scale. we evaluated biodegradation of pyrene by mycobacterium vanbaalenii pyr-1 as a function of sediment particle sizes, and investigated the relationship between the rate of degradation on sand, silt and clay particles with their indiv ... | 2011 | 20714921 |
| treatment of latent infection with mycobacterium tuberculosis: update 2010. | much remains unknown about latent infection with mycobacterium tuberculosis. existing immunodiagnostic tools for this condition have various limitations, most importantly in their ability to predict disease. randomised controlled trials have established protective efficacy of isoniazid therapy for 6-12 months among non-hiv-infected and hiv-infected subjects. while efficacy may reach 90%, acceptance and adherence to prolonged therapy are less than desired. rifampicin plus pyrazinamide for 2 month ... | 2011 | 20693257 |
| peripheral n-methyl-d-aspartate receptors contribute to mechanical hypersensitivity in a rat model of inflammatory temporomandibular joint pain. | the aim of this study was to determine whether peripheral n-methyl-d-aspartate (nmda) receptors are involved in inflammation-induced mechanical hypersensitivity of the temporomandibular joint (tmj) region. we developed a rat model of mechanical sensitivity to complete freund's adjuvant (cfa; 2μl containing 1μg mycobacterium tuberculosis)-induced inflammation of the tmj and examined changes in sensitivity following injection of nmda receptor antagonists (dl-2-amino-5-phosphonovaleric acid (ap5) o ... | 2011 | 20675160 |
| hypersensitivity pneumonitis and metalworking fluids contaminated by mycobacteria. | metalworking fluids (mwf) are responsible for hypersensitivity pneumonitis (hp). the aim of the present study was to identify the antigen (ag) responsible for mwf-associated hp, and to optimise serological diagnosis by definition of a threshold allowing discrimination between hp patients and asymptomatic exposed workers. 13 patients, who were workers at a car engine manufacturing plant, were suspected of mwf-associated hp. microbial analysis of 83 used mwfs was carried out. sera from 13 mwf-asso ... | 2011 | 20693254 |
| immunogenicity of mycobacterium avium subsp. paratuberculosis proteins in crohn's disease patients. | the association between mycobacterium avium subsp. paratuberculosis (map) and crohn's disease (cd) is supported by several studies reporting the detection or isolation of map from human tissues, but a direct association is still debatable. | 2011 | 20735153 |
| a longitudinal study to evaluate the diagnostic potential of a direct faecal quantitative pcr test for johne's disease in sheep. | a longitudinal study was carried out to evaluate the diagnostic potential of the previously developed direct faecal real-time quantitative pcr (qpcr) assay (kawaji et al., 2007) for the detection of mycobacterium avium subsp. paratuberculosis (map) infected sheep. of the 58 sheep, 38 were orally inoculated with map, while 20 controls were maintained separately from the infected group throughout the trial. all animals were tested by qpcr, faecal culture and serum elisa pre-inoculation and at 4, 8 ... | 2011 | 20729013 |
| hijacked phagosomes and leukocyte activation: an intimate relationship. | intracellular pathogens have developed different strategies to survive within host cells. for example, these pathogens might interfere with the biogenesis of phagolysosomes, thereby forming replicative vacuoles. although the complex mechanisms used by pathogens to hijack the biogenesis of phagolysosomes have been elucidated in naive leukocytes, the role of leukocyte activation in this process has not yet been investigated. leukocytes are known to be activated by cytokines, and several reports ha ... | 2011 | 20720162 |
| mixed infections of corynebacterium pseudotuberculosis and non-tuberculous mycobacteria in south african antelopes presenting with tuberculosis-like lesions. | routine meat inspection of antelope carcasses from a south african game reserve revealed a high prevalence of tuberculosis-like lesions. this study aimed to identify the causative agent of this disease and to describe its pathological features. in total, 139 antelopes were randomly harvested from the game reserve and subjected to meat inspection. of these animals, 46 (33%) showed gross visible, tuberculosis-like lesions. histopathological examination revealed the presence of encapsulated necrogr ... | 2011 | 20727687 |
| tuberculosis disease diagnosis using artificial neural network trained with genetic algorithm. | tuberculosis is a common and often deadly infectious disease caused by mycobacterium; in humans it is mainly mycobacterium tuberculosis (wikipedia 2009). it is a great problem for most developing countries because of the low diagnosis and treatment opportunities. tuberculosis has the highest mortality level among the diseases caused by a single type of microorganism. thus, tuberculosis is a great health concern all over the world, and in turkey as well. this article presents a study on tuberculo ... | 2011 | 20703557 |
| antioxidant, antitumor and antimicrobial activities of the crude extract and compounds of the root bark of allanblackia floribunda. | context: allanblackia floribunda oliver (guttiferae) is an african medicinal plant used traditionally to treat a variety of ailments. objective: we investigated the antitumor, radical scavenging, antimycobacterial, antibacterial and antifungal activities of the root bark extract of a. floribunda and three isolated phenolics, namely 1,7-dihydroxyxanthone (1), morelloflavone (2) and 7'-o-glucoside of morelloflavone (3). materials and methods: the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (dpph) radi ... | 2011 | 20738222 |
| molecular characterization of mycobacterium tuberculosis complex (mtbc) isolated from cattle in northeast and northwest china. | we studied throat swabs and corresponding serum samples collected from 1067 protein purified derivative (ppd)-tuberculin skin test (tst) positive cattle from different regions of china. the 1067 throat swabs were inoculated onto modified löwenstein-jensen medium for the isolation and culture of mycobacteria. acid-fast bacilli were identified using traditional biochemical methods, polymerase chain reaction (pcr) amplification and multiplex pcr. they were distinguished as mycobacterium tuberculosi ... | 2011 | 20797738 |
| serial testing with the interferon-γ release assay in portuguese healthcare workers. | evidence for the utility of the new mycobacterium tuberculosis (mtb) specific ifn-γ release assays in diagnosing latent tuberculosis infection (ltbi) is growing. however, data concerning conversion and reversion rates in serial testing of healthcare workers (hcws) with an interferon-γ release assay are sparse. | 2011 | 20721576 |
| factors impacting the results of interferon-γ release assay and tuberculin skin test in routine screening for latent tuberculosis in patients with inflammatory bowel diseases. | screening for latent tuberculosis (ltb) including chest x-ray, tuberculin skin test (tst), and facultative whole blood interferon-γ assay (igra) is part of routine management in inflammatory bowel disease (ibd) patients before starting therapy with tumor necrosis factor (tnf)-α inhibitors. however, in patients with immunomodulators (im) tst and igra might show limitations. | 2011 | 20722065 |
| first-time detection of mycobacterium species from goats in ethiopia. | tuberculosis (tb) is an important zoonosis affecting a wide range of hosts. an abattoir study was conducted on 1,536 randomly selected male goats slaughtered at modjo modern export abattoir to determine the prevalence of tuberculosis in slaughtered goats. carcasses and organs of all the study animals were first examined by routine meat inspection followed by detailed meat inspection. samples from tuberculous lesions were cultured for mycobacterial isolation and identification. histopathology was ... | 2011 | 20725858 |
| mendelian susceptibility to mycobacterial disease. | the host response to mycobacterial infection is mediated by the type i cytokine pathway (cell-mediated immunity). deficiencies in this response result in susceptibility to poorly pathogenic mycobacterial species such as bacille calmette-guérin and environmental mycobacteria. in recent years a number of mutations in the genes encoding major components in the type i cytokine axis have been described which predispose to disseminated infection with these weakly virulent mycobacterial species. affect ... | 2011 | 20718793 |
| diagnosing tb infection in children: analysis of discordances using in vitro tests and the tuberculin skin test. | the aim of the present study was to compare the performance of the interferon (ifn)-γ tests (quantiferon®-tb gold in-tube (qft-g-it) and t-spot®.tb) with the tuberculin skin test (tst) in diagnosing tuberculosis (tb) infection in children, and to analyse discordant results. this was a prospective study including 98 children from contact-tracing studies and 68 children with tst indurations ≥5 mm recruited during public health screenings. positive ifn-γ tests results were associated with risk of e ... | 2011 | 20729220 |
| a theoretical assessment of the effects of smoking on the transmission dynamics of tuberculosis. | smoking has long being associated with tuberculosis. we present a tuberculosis dynamics model taking into account the fact that some people in the population are smoking in order to assess the effects of smoking on tuberculosis transmission. the epidemic thresholds known as the reproduction numbers and equilibria for the model are determined and stabilities analyzed. qualitative analysis of the model including positivity and persistence of solutions are presented. the model is numerically analyz ... | 2011 | 20725798 |
| nontuberculous mycobacterial infection as a cause of difficult-to-control asthma: a case-control study. | symptomatic disease due to nontuberculous mycobacteria (ntm) is known to occur commonly in the presence of structural lung disease, but is not described in association with asthma. | 2011 | 20829338 |
| mycobacterium tuberculosis infection of a native polycystic kidney following renal transplantation. | tuberculosis is a recognized complication following renal transplantation. patients with autosomal-dominant polycystic kidney disease are increasingly being offered renal transplantation as an alternative to chronic hemodialysis. these patients are uniquely susceptible to serious upper urinary tract infections that are associated with significant morbidity and mortality. while involvement with gram-negative organisms is well described, mycobacterial infection of native polycystic kidneys after t ... | 2011 | 20825590 |
| the interleukin 10 response in ovine johne's disease. | johne's disease is an enteric mycobacterial infection of ruminants that has significant global economic impact. the classic host reaction is one of an early t-cell mediate immune response, with predominant interferon gamma (ifnγ) activity; there is subsequent lowering of this response as animals reach the terminal stages of disease. interleukin (il)-10, which can suppress th1-type and enhance th2-type cytokine production, is considered to play a role in the later stages of johne's disease. to de ... | 2011 | 20817276 |
| hypercytokinaemia accompanies hiv-tuberculosis immune reconstitution inflammatory syndrome. | increased access to combination antiretroviral therapy in areas co-endemic for tuberculosis (tb) and hiv-1 infection is associated with an increased incidence of immune reconstitution inflammatory syndrome (tb-iris) whose cause is poorly understood. a case-control analysis of pro- and anti-inflammatory cytokines in tb-iris patients sampled at clinical presentation, and similar control patients with hiv-tb prescribed combined antiretroviral therapy who did not develop tb-iris. peripheral blood mo ... | 2011 | 20817712 |
| [multidrug-resistant tuberculosis management in three french hospitals]. | multidrug-resistant tuberculosis (mdr-tb) management is based on specific who guidelines. | 2011 | 20801589 |
| mycobacterium tuberculosis rv1090 and rv1987 encode functional β-glucan-targeting proteins. | mycobacterium tuberculosis is a facultative intracellular pathogen, and the ability of this bacterium to survive and to grow inside macrophages is central to its virulence. multiple strategies are employed by m. tuberculosis to ensure survival in macrophages, including secretion of several proteins, which are good candidates to be virulence factors, drug targets for disease intervention, and vaccine antigens. however, some m. tuberculosis secreted proteins do not appear to play any role in the g ... | 2011 | 20826214 |
| can an immunohistochemistry method differentiate intestinal tuberculosis from crohn's disease in biopsy specimens? | it is sometimes difficult to diagnose whether a patient has intestinal tuberculosis or crohn's disease because both have similar clinical, pathologic, and endoscopic features. however, their therapies are completely different and a mistake in diagnosis can result with deterioration. many laboratory methods for the diagnosis of tuberculosis require considerable time to receive a diagnostic result. we wanted to evaluate whether an immunohistochemical tuberculosis staining method can be helpful for ... | 2011 | 20824497 |
| the cost of medical management of pulmonary nontuberculous mycobacterial disease in ontario, canada. | treatment of pulmonary nontuberculous mycobacterial (ntm) infection is complex, requiring multiple antibiotics and a prolonged treatment course. we determined the monthly cost of treating patients with pulmonary ntm infections in our clinic, a tertiary care centre in toronto, ontario, canada. we reviewed records of a single clinic at the university health network (toronto) for all patients with pulmonary ntm isolates. pharmacological and nonpharmacological treatment costs were calculated using a ... | 2011 | 20817704 |
| detection of mycobacterium avium subsp. paratuberculosis (map)-specific is900 dna and antibodies against map peptides and lysate in the blood of crohn's disease patients. | 2011 | 20815038 | |
| effects of photodynamic therapy on rapidly growing nontuberculous mycobacteria keratitis. | the authors investigated the antimicrobial effect of methylene blue (mb)-mediated photodynamic therapy (pdt) on mycobacterium fortuitum keratitis. | 2011 | 20811055 |
| bystander inhibition of dendritic cell differentiation by mycobacterium tuberculosis-induced il-10. | mycobacterium tuberculosis (mtb) evades the immune response by impairing the functions of different antigen-presenting cells. we have recently shown that mtb hijacks differentiation of monocytes into dendritic cells (dcs). to further characterize the mechanisms underlying this process, we investigated the consequences of inducing dendritic cell differentiation using interferon-α and granulocyte-macrophage colony-stimulating factor in the presence of supernatants (sns) obtained from monocyte cult ... | 2011 | 20805841 |
| noodling and mycobacterium marinum infection mimicking seronegative rheumatoid arthritis complicated by anti-tumor necrosis factor α therapy. | 2011 | 20806275 | |
| performance of a whole-blood interferon-gamma release assay with mycobacterium rd1-specific antigens among hiv-infected persons. | to evaluate the usefulness of one of igras, quantiferon-tb gold (qft-g), in human immunodeficiency virus- (hiv-) infected patients with various cd4(+) t cell counts. | 2011 | 20814593 |
| maximizing the native concentration and shelf life of protein: a multiobjective optimization to reduce aggregation. | a multiobjective optimization was performed to maximize native protein concentration and shelf life of asd, using artificial neural network (ann) and genetic algorithm (ga). optimum ph, storage temperature, concentration of protein, and protein stabilizers (glycerol, nacl) were determined satisfying the twin objective: maximum relative area of the dimer peak (native state) after 48 h of storage, and maximum shelf life. the relative area of the dimer peak, obtained from size exclusion chromatogra ... | 2011 | 20798936 |
| a fatal mycobacterium chelonae infection in an immunosuppressed patient with systemic lupus erythematosus and concomitant fahr's syndrome. | we present a case of systemic mycobacterium chelonae infection in an immunosuppressed patient with systemic lupus erythematosus (sle), idiopathic hypoparathyroidism, and hypothyroidism. the patient was treated for 3 months for skin infection with clarithromycin monotherapy. since her condition deteriorated, the antibiotic therapy was switched to intravenously administered clindamycin, cloxacillin, and meropenem. due to further deterioration and isolation of m. chelonae from the blood culture, an ... | 2011 | 20803049 |
| similar changes of hypothalamic feeding-regulating peptides mrnas and plasma leptin levels in pthrp-, lif-secreting tumors-induced cachectic rats and adjuvant arthritic rats. | parathyroid hormone-related protein (pthrp) is a causative factor of humoral hypercalcemia in malignancy. however, it is difficult to explain the mechanism of anorexia/cachexia with pthrp secretion in detail. previously, we demonstrated that the expressions of orexigenic peptides increased and anorexigenic peptides decreased under cachectic conditions in rats carrying tumors secreting pthrp. in this study, we investigated whether such changes in the expression of hypothalamic feeding-regulating ... | 2011 | 20602340 |
| clinical evaluation of a homemade enzyme-linked immunospot assay for the diagnosis of active tuberculosis in china. | the rapid diagnosis of smear-negative pulmonary tuberculosis (tb) and extrapulmonary tb is a significant problem in clinical practice. we evaluated the usefulness of a homemade enzyme-linked immunospot (elispot) assay for the diagnosis of active tb in china. seventy-eight healthy volunteers, 60 patients with active tb, and 32 patients with non-tb diseases were evaluated by tuberculin skin test (tst), an elispot assay using a recombinant cfp-10/esat-6 fusion protein (rcfp-10/esat-6) as a stimulan ... | 2011 | 20596901 |
| identification of hotspot regions of murb oxidoreductase enzyme using homology modeling, molecular dynamics and molecular docking techniques. | despite the availability of effective chemotherapy and a moderately protective vaccine, new anti-tuberculosis agents are urgently needed to decrease the global incidence of tuberculosis (tb) disease. the murb gene belongs to the bacterial cell wall biosynthesis pathway and is an essential drug target in mycobacterium tuberculosis (mtb) that has no mammalian counterparts. here, we present an integrated approach involving homology modeling, molecular dynamics and molecular docking studies on mtb-m ... | 2011 | 20614148 |
| culture of mycobacterium avium subspecies paratuberculosis (map) from blood and extra-intestinal tissues in experimentally infected sheep. | mycobacterium avium subspecies paratuberculosis (map) is the causative agent of johne's disease or paratuberculosis, a chronic enteritis of ruminants, and has been suggested to play a role in crohn's disease in humans. while johne's disease is primarily expressed in the gastrointestinal tract, isolation of map from extra-intestinal tissues indicates that microbial dissemination via the haematogenous route may occur during the infection. consequently, the occurrence of mycobacteraemia and dissemi ... | 2011 | 20609533 |
| occurrence of mycobacterium spp. and other pathogens in lymph nodes of slaughtered swine and wild boars (sus scrofa). | mycobacterium spp. and other pathogens were investigated in 258 swine lymph nodes (129 with and 129 without apparent lesions), and 120 lymph nodes (60 with and 60 without lesions) from wild boars (sus scrofa). a total of lymph nodes from swine and wild boars were collected of different animals. submaxillar and mesenteric lymph nodes were submitted to microbiological examination and colonies suggestive of mycobacterium spp. (alcohol-acid bacilli) were submitted to pcr restriction assay (pra). in ... | 2011 | 20621319 |
| expression and characterization of mycobacterium tuberculosis cyp144: common themes and lessons learned in the m. tuberculosis p450 enzyme family. | cyp144 from mycobacterium tuberculosis was expressed and purified. cyp144 demonstrates heme thiolate coordination in its ferric form, but the cysteinate is protonated to thiol in both the carbon monoxide-bound and ligand-free ferrous forms (forming p420 in the former). tight binding of various azole drugs was shown, with affinity for miconazole (k(d)=0.98 μm), clotrimazole (0.37 μm) and econazole (0.78 μm) being highest. these azoles are also the trio with the highest affinity for the essential ... | 2011 | 20621636 |
| antitubercular resorcinol analogs and benzenoid c-glucoside from the roots of ardisia cornudentata. | bioassay-guided fractionation of the roots of ardisia cornudentata mez (myrsinaceae) led to the isolation of three new compounds, 3-methoxy-2-methyl-5-pentylphenol (1), 3-methoxy-2-methyl-5-(1'-ketopentyl)phenol (2), and cornudoside (3), together with twenty-six known compounds. their structures were elucidated by analysis of spectroscopic data. thirteen of these isolates, 1, 2, 4- 6, 9- 15, and 21 showed antitubercular activities against mycobacterium tuberculosis h37r (v) in vitro, with mic va ... | 2011 | 20607650 |
| susceptibility of rapidly growing mycobacteria isolated from cats and dogs, to ciprofloxacin, enrofloxacin and moxifloxacin. | rapidly growing mycobacteria (rgm) cause infections in cats and dogs which require prolonged antibacterial medication for resolution. in australia, pathogens from the mycobacterium fortuitum and mycobacterium smegmatis clusters are responsible for most of the rgm infections in cats and dogs. as fluoroquinolones are often recommended for treating such infections, 14 m. fortuitum isolates, 51 isolates from the m. smegmatis cluster and 2 m. mageritense isolates, collected from feline and canine pat ... | 2011 | 20619975 |
| infection of eurasian badgers (meles meles) with mycobacterium avium complex (mac) bacteria. | there are few reports of infection with mycobacterium avium complex (mac) bacteria in badgers. in this study archive data relating to the isolation of mac organisms from badgers in the uk is presented, and information derived from recent cases of such infection in spain is used to illustrate the associated pathology and to characterise strain types. tissue samples were cultured for mycobacteria and, in the case of spanish badgers, were examined both histopathologically and using immunohistochemi ... | 2011 | 20605496 |
| skin and soft-tissue infection caused by non-tuberculous mycobacteria in taiwan, 1997-2008. | the aim of this study was to investigate the clinical, microbiological, and pathological characteristics and the outcomes of skin and soft-tissue infection (ssti) caused by non-tuberculous mycobacteria (ntm). medical records of 50 patients with ssti caused by ntm identified from 2005 to 2008 and 63 patients previously reported in a medical centre from 1997 to 2004 were reviewed. the annual incidence (per 100,000 outpatients and in-patients) ranged from 0·57 in 2005, 0·38 in 2007, to 1·1 in 2008, ... | 2011 | 20598210 |
| relationship between mutation of serine residue at 315th position in m. tuberculosis catalase-peroxidase enzyme and isoniazid susceptibility: an in silico analysis. | remarkable advances have been made in the drug therapy of tuberculosis. however much remains to be learned about the molecular and structural basis of drug resistance in mycobacterium tuberculosis. it is known that, activation of isoniazid (inh) is mediated by mycobacterium tuberculosis catalase-peroxidase (mtbkatg) and mutation at position 315 (serine to threonine) leads to resistance. we have conducted studies on the drug resistance through docking and binding analysis supported by time-scale ... | 2011 | 20593210 |
| the sensor kinase mprb is required for rhodococcus equi virulence. | rhodococcus equi is a soil bacterium and, like mycobacterium tuberculosis, a member of the mycolata. through possession of a virulence plasmid, it has the ability to infect the alveolar macrophages of foals, resulting in pyogranulomatous bronchopneumonia. the virulence plasmid has an orphan two-component system (tcs) regulatory gene, orf8, mutation of which completely attenuates virulence. this study attempted to find the cognate sensor kinase (sk) of orf8. annotation of the r. equi strain 103 g ... | 2011 | 20637548 |
| [sporotrichoid topographic distribution and mycobacterium marinum grown on a subdermal contraceptive implant]. | we report a 37-year-old woman who presented a cutaneous papulonodular skin eruption with sporotrichoid topographic distribution. the diagnosis of mycobacterium marinum infection was obtained with the bacteriological examination of a cutaneous biopsy and related to cleaning her aquarium at home. mycobacteriological grown on a subdermal contraceptive implant had not been published before. | 2011 | 20646797 |
| mycobacterium lentiflavum--a cause of infections in the head and neck: case report and literature review. | 2011 | 20674119 | |
| epidemiological investigation of a mycobacterium avium subsp. hominissuis outbreak in swine. | mycobacterium avium subsp. hominissuis (mah) infection in swine may cause granulomatous lesions in lymph nodes that must undergo differential diagnosis with those caused by m. tuberculosis complex members. moreover, mah outbreaks can lead to severe economic losses due to condemnation of carcasses. a number of potential sources of infection for animals can usually be identified in contaminated farms. this report describes the application of several molecular characterization techniques in order t ... | 2011 | 20653991 |
| combination of molecular assay and clinical evaluation for early confirmation of tuberculosis cases. | the cost-effectiveness of the probetec et direct tb assay (dtb) was compared with that of culture for detection of mycobacterium tuberculosis complex in 361 acid-fast stain-positive respiratory specimens. the overall sensitivity, specificity, positive predictive value and negative predictive value of dtb were 97.7%, 86.6%, 87.2% and 97.6%, respectively. when clinical evaluation was added to dtb, the specificity and positive predictive value of dtb increased to 94.7% and 95.4%, respectively. trea ... | 2011 | 20636424 |
| mycobacterium avium ssp. hominissuis biofilm is composed of distinct phenotypes and influenced by the presence of antimicrobials. | mycobacterium avium ssp. hominissuis, hereafter referred to as m. avium, forms biofilm, a property that, in mice, is associated with lung infection via aerosol. as m. avium might co-inhabit the respiratory tract with other pathogens, treatment of the co-pathogen-associated infections, such as in bronchiectasis, would expose m. avium to therapeutic compounds that may have their origin in other organisms sharing the natural environments. incubation of m. avium with two compounds produced by enviro ... | 2011 | 20636426 |
| gastrointestinal tuberculosis in renal transplant recipients: case report and review of the literature. | mycobacterium tuberculosis is an important opportunistic pathogen following renal transplantation and is often associated with adverse outcomes. gastrointestinal tuberculosis (gitb) is an infrequent manifestation of tb but a potentially lethal one. we present a case of a renal allograft recipient with gitb 18 months after transplant and review other published cases to identify the typical presenting symptoms, risk factors, and natural history. treatment of gitb is also discussed. | 2011 | 20626712 |
| [retropharyngeal abscess.] | 2011 | 20667628 | |
| the novel responses of ethambutol against mycobacterium smegmatis mc²155 revealed by proteomics analysis. | ethambutol (emb), one of the effective anti-mycobacterial drugs, inhibits the biosynthesis of mycobacterium cell wall. to elucidate the molecular mechanism of emb against tuberculosis (tb), mycobacterium smegmatis mc²155 was employed as a model of mycobacterial system in this study. we compared the protein profiles on m. smegmatis mc²155 treated by emb and untreated using fluorescence difference two-dimensional gel electrophoresis (2-d dige). a total of 40 differential protein spots were selecte ... | 2011 | 20686769 |
| unusual nontuberculous mycobacterium infection of the rectum. | 2011 | 20674915 | |
| disseminated mycobacterium genavense with pulmonary nodules in a kidney transplant recipient: case report and review of the literature. | we report a case of disseminated mycobacterium genavense with pulmonary nodules in a kidney transplant recipient and review the associated literature. disease caused by m. genavense has been recognized in acquired immunodeficiency syndrome (aids) patients since 1990, with subsequent case reports in other immunocompromised host populations. in aids patients, pulmonary lesions are an uncommon finding. this is the first report to our knowledge of a human immunodeficiency virus (hiv)-negative patien ... | 2011 | 20663117 |
| an outbreak of mycobacterium mucogenicum bacteremia in pediatric hematology-oncology patients. | mycobacterium mucogenicum (mm) is a rapidly growing nontuberculous mycobacterium that is commonly identified in tap water that can rarely cause bacteremia. we describe an outbreak of mm bacteremia among pediatric hematology-oncology patients. | 2011 | 20651636 |
| prevalence and concentration of non-tuberculous mycobacteria in cooling towers by means of quantitative pcr: a prospective study. | there is an increasing level of interest in non-tuberculous mycobacteria (ntm) due to the increasing reported rates of diseases caused by them. although it is well known that ntm are widely distributed in the environment it is necessary to identify its reservoirs to prevent possible infections. in this study, we aimed to investigate the occurrence and levels of ntm in cooling towers to provide evidences for considering these settings as possible sources of respiratory infections. in the current ... | 2011 | 20640853 |
| an all-consuming case. | this is a case of a 6-month-old child who presented to the local paediatric department with a relatively short history of developmental regression, hypotonia, weight loss, irritability and hepatosplenomegaly. the child then proceeded to spike temperatures up to 39°c. the case demonstrates the logical investigative process involved in such a case and discusses the differential diagnoses at each stage. it also highlights the importance of multi-specialty work in complex cases and the potential con ... | 2011 | 20675523 |
| study of interactions between mycobacterium tuberculosis proteins: sigk and anti-sigk. | the development of novel antituberculosis therapeutic molecules is a global health concern. complex gene expression in mycobacterium tuberculosis is mediated mainly by various sigma factors. the sigk protein binds to rna polymerase, facilitating the expression of genes encoding the antigenic proteins mpt70 and mpt83. the anti-sigk protein is a negative regulator of sigk and inhibits the initiation of transcription. this study focuses on the interactions between sigk and the n-terminal domain of ... | 2011 | 20676709 |
| leprosy in transplant recipients: report of a case after liver transplantation and review of the literature. | leprosy still is an important public health problem in several parts of the world including brazil. unlike the diseases caused by other mycobacteria, the incidence and clinical presentation of leprosy seems little affected in immunosuppressed patients. we report the first case, to our knowledge, of a liver transplant patient who developed multi-bacillary leprosy. the patient presented with papules and infiltrated plaques with loss of sensation suggestive of leprosy 3.5 years after living-related ... | 2011 | 20678090 |
| supralevator fistula-in-ano in tuberculosis. | perianal mycobacterium tuberculosis is rare, but tuberculosis (tb) is now endemic in many areas of the world. it is essential to recognize tb to institute appropriate management. we report three cases of tb presenting with supralevator abscess. | 2011 | 19863596 |
| tuberculosis infection in animal and human populations in three districts of western gojam, ethiopia. | tuberculosis concurrent infection in cattle and their respective owners in north-western ethiopia had been investigated. two hundred and ten cattle owners and 1220 heads of their cattle were included in the study to determine degree of tuberculosis infection in cattle owned by tuberculosis patients and tuberculosis patients. comparative intradermal tuberculin test, bacteria culturing, acid fast staining and biochemical tests were used to conduct the study. the prevalence of tuberculosis was sign ... | 2011 | 19912606 |
| two cases of disseminated mycobacterium avium infection associated with a new immunodeficiency syndrome related to cxcr4 dysfunctions. | disseminated mycobacterium avium complex (mac) infection is a rare but severe disease mostly seen in patients with aids. it has been previously described in patients suffering from other kinds of immunodeficiency (e.g. primary immunodeficiency diseases in children or hairy cell leukaemia). we report two cases of disseminated mac disease in young women with extended granulomatosis that revealed a new form of severe immunodeficiency syndrome. both clinical observations initially appeared to be ver ... | 2011 | 20148920 |
| development of a loop-mediated isothermal amplification assay for the detection of mycobacterium bovis. | bovine tuberculosis caused by mycobacterium bovis is an important zoonosis. in this study, a simple, rapid method for detecting this organism was developed based on loop-mediated isothermal amplification of the mpt83 gene. the technique will be of value in the clinical and field-based diagnosis of m. bovis and can differentiate it from other bacteria such as corynebacterium diphtheriae, streptococcus pneumoniae, β-haemolytic streptococci, pseudomonas aeruginosa, yersinia pseudotuberculosis, stap ... | 2011 | 20138787 |
| adverse effects of multidrug-resistant tuberculosis treatment with a standardized regimen: a report from iran. | compared with the treatment of drug-sensitive tuberculosis, the treatment of multidrug-resistant tuberculosis (mdr-tb) is more difficult. this study was conducted at the national referral center of tuberculosis in tehran, iran, to evaluate adverse drug reactions of treatment of mdr-tb. from 2006 to 2009, all patients admitted into masih daneshvari hospital in tehran, iran, for mdr-tb were considered for this study. the standard treatment for mdr-tb consisted of amikacin, prothionamide, ofloxacin ... | 2011 | 20019591 |
| using bcg, mpt-51 and ag85 as antigens in an indirect elisa for the diagnosis of bovine tuberculosis. | this study evaluated the mycobacterium tuberculosis protein antigen mpt-51, the trimeric antigen 85 (ag85) complex, and bacillus calmette-guérin (bcg) in an indirect elisa to diagnose bovine tuberculosis (tb) from serum samples. serum was collected from 208 intra-dermal tuberculin test (itt)-positive and 54 itt-negative animals from a region where bovine tb is endemic. using the ag85 and bcg antigens, the indirect elisa was able to discriminate itt-positive from itt-negative animals. this level ... | 2011 | 20022773 |
| facial tuberculoid leprosy: case report. | leprosy is a chronic, infectious, systemic disease caused by mycobacterium leprae and is classified as paucibacillary and multibacillary types. it is contagious and has an insidious onset. clinical presentation is characterised by hypopigmented skin lesions with reduced sensation. presence of acid-fast bacilli in tissue specimens is regarded as a gold standard for diagnosis. treatment is based on multi-drug regimens. we report a case of borderline tuberculoid leprosy in a 31-year-old woman. | 2011 | 20188445 |
| a novel method for the rapid and prospective identification of beijing mycobacterium tuberculosis strains by high-resolution melting analysis. | genotypic analysis of mycobacterium tuberculosis (mtb) has enabled the definition of several lineages. the beijing family, which is considered highly virulent and transmissible, has been associated with resistance in certain settings and involved in severe outbreaks, making it one of the most closely-monitored lineages. therefore, rapid prospective identification of beijing mtb strains could be relevant. in the present study, we evaluate a real-time pcr followed by high-resolution melting (hrm) ... | 2011 | 20384709 |
| tuberculosis of the breast in a patient undergoing hemodialysis. | a 61-year-old female patient with diabetes undergoing maintenance hemodialysis presented with fever and swelling of her right breast. she had been unresponsive to nonspecific antimicrobial therapies. breast tuberculosis and tuberculous lymphadenitis were diagnosed by excision biopsy and tissue culture. a combination antituberculous treatment including isoniazid, rifampin, ethambutol, and pyrazinamide was started and resulted in the remission of the lesions by the end of the first month of therap ... | 2011 | 20364402 |
| bovine tuberculosis: making a case for effective surveillance. | in 2008, a cow with marked gross lesions suspicious for bovine tuberculosis (btb) was identified by meat inspection at home slaughtering in north-western germany. epidemiological investigations led to the identification of another 11 affected farms with a total of 135 animals which reacted positive to the skin test. eight affected farms had been in trade contact with the putative index farm. while the source for the initial introduction remained unknown, it was shown that all isolates tested sha ... | 2011 | 20392304 |
| synchronous infection with mycobacterium chelonae and paecilomyces in a heart transplant patient. | a 41-year-old male who was 3 years status post heart transplant presented with a 3-month history of painful erythematous nodules and ulcers on his lower legs and right hand. first, mycobacterium chelonae infection was revealed through several biopsies with molecular sequence analysis, and combination treatment, including clarithromycin, was started. during the treatment, lesions of the legs showed an improvement, but a fluctuant erythematous nodule on the thumb did not respond. repetitive biopsy ... | 2011 | 20412536 |
| development of a sarcoidosis murine lung granuloma model using mycobacterium superoxide dismutase a peptide. | sarcoidosis is characterized by noncaseating granulomas containing cd4(+) t cells with a th1 immunophenotype. although the causative antigens remain unknown, independent studies noted molecular and immunologic evidence of mycobacterial virulence factors in sarcoidosis specimens. a major limiting factor in discovering new insights into the pathogenesis of sarcoidosis is the lack of an animal model. using a distinct superoxide dismutase a peptide (soda) associated with sarcoidosis granulomas, we d ... | 2011 | 20348207 |
| mycobacterium fortuitum infections associated with laparoscopic gastric banding. | mycobacterium fortuitum, a rapidly growing atypical mycobacteria, is commonly found in soil and water. this organism is most often known to cause skin, bone, and soft tissue infections associated with local trauma, surgical procedures, and in patients with immunodeficiency. nosocomial infections associated with a variety of contaminated devices and equipment have also been widely documented. this report presents the first cases of m. fortuitum infection following laparoscopic gastric banding pro ... | 2011 | 20336391 |
| incision infection with mycobacterium tuberculosis after total hip arthroplasty without any primary tuberculosis focus. | we describe a case of a tuberculous abscess unassociated with other clinical features of tuberculosis in a 46-year-old woman with a history of 4 hip surgeries plus total hip arthroplasty (tha) due to developmental hip dislocation. four months after tha, she developed a collection at the incision site for which specimens produced positive culture findings for mycobacterium tuberculosis. we could not detect any primary focus of tuberculosis anywhere in the patient's body. we performed soft-tissue ... | 2011 | 20304601 |
| predictive models for nucleoside bisubstrate analogs as inhibitors of siderophore biosynthesis in mycobacterium tuberculosis: pharmacophore mapping and chemometric qsar study. | inhibitors of aryl acid adenylating enzymes (aaae), known as mbta, involved in siderophore biosynthesis in mycobacterium tuberculosis, are being explored as potential antitubercular agents. in this article, we report the development of a robust pharmacophore model and investigation of structure-activity relationship of several nucleoside bisubstrate analogs reported as mbta inhibitors. the developed pharmacophore model revealed the importance of two hydrogen bond donors and one hydrogen bond acc ... | 2011 | 20306296 |
| respiratory symptoms in a patient on anti-tumour necrosis factor therapy; beware the negative enzyme linked immuno-spot (elispot) in suspected mycobacterial disease. | 2011 | 20504863 | |
| phylogenetic analysis and prokaryotic expression of nmaap1 derived from bcg-activated murine macrophages. | bcg-activated macrophages exerted anti-tumor activities. cell surface molecules play an important role in mediating endocytosis by macrophages. in the previous study, we identified a group of 454 membrane proteins specifically expressed on bcg-activated mouse macrophages, including a protein named nmaap1 (novel macrophage activated associated protein). in this study, we aligned the full-length nucleotide sequences of nmaap1 and its homologous sequences to construct its phylogenetic tree, and clo ... | 2011 | 20478616 |
| hansen's disease (leprosy) complicated by secondary mycobacterial infection. | a patient with hansen's disease received corticosteroids for a type 1 leprosy reaction and subsequently developed a new cutaneous lesion at the original biopsy site from which mycobacterium fortuitum was cultured. a review of the literature found only two other cases of coinfection with atypical mycobacteria and mycobacterium leprae, although there are many reports of pulmonary tuberculosis in patients with leprosy. this case highlights the diagnostic difficulties encountered when a patient has ... | 2011 | 20494478 |
| identification of loci associated with tolerance to johne's disease in holstein cattle. | johne's disease, caused by mycobacterium avium subspecies paratuberculosis (map), is a fatal disease in cattle. the objective of this study was to identify loci associated with tolerance in cows infected with map. tolerance was defined as a cow's fitness at a given level of map infection intensity. fitness was measured by map faecal cultures, and map infection intensity was measured by culturing four gut tissues. the quantitative phenotype of tolerance was defined by numerical indexes of culture ... | 2011 | 20477805 |
| concomitant mycobacterium avium infection and hodgkin's disease in a lymph node from an hiv-negative child. | we report a case of an immunocompetent child with simultaneously an infection with mycobacterium avium and hodgkin's disease in a cervical lymph node. a positive pcr result for m. avium on a biopsy of the lymph node directed the definitive diagnosis for both etiologies and avoided a possible dissemination of this infection after chemotherapy was started. | 2011 | 20467849 |
| cutaneous non-tuberculous mycobacterial infections: a clinical and histopathological study of 17 cases from lebanon. | only a few studies characterized cutaneous non-tuberculous mycobacterium (ntm) infections in this region of the world. objective the aim of this study was to describe the epidemiological, clinical and histological findings of cutaneous ntm infections in lebanon. | 2011 | 20456544 |
| clinical applicability of t-cell interferon-a release assay for tumour necrosis factor-a inhibitor therapy in severe psoriasis. | many studies have found that screening and treatment of latent tuberculosis (tb) before starting treatment with tumour necrosis factor (tnf)-a inhibitors reduces associated tb infections. the new t-cell interferon-a release assay (tigra), is more specific and sensitive for detection of latent tb compared with the tuberculin skin test (tst). we report results of tigra in our first 63 patients commencing tnf-a inhibitors for severe psoriasis. of the 63 patients, 5 (7.9%) had a positive tigra resul ... | 2011 | 20456401 |
| impact of malnutrition on clinical presentation, clinical course, and mortality in mdr-tb patients. | despite the adoption of strategies to prevent and treat multidrug-resistant tuberculosis (mdr-tb) over the past decade, latvia continues to have one of the highest rates of mdr-tb in the world. it is important to identify modifiable factors that may impact on mdr-tb patient outcomes. a study was conducted to elucidate the association between nutritional status and clinical presentation, clinical course, and mortality in 995 adult patients treated for mdr-tb from 2000 to 2004. twenty percent of p ... | 2011 | 20429966 |
| a major subgroup of beijing family mycobacterium tuberculosis is associated with multidrug resistance and increased transmissibility. | this study investigated further the association between the beijing family mycobacterium tuberculosis circulating in rural china and anti-tuberculosis (tb) drug resistance. in total, 351 m. tuberculosis isolates were collected through a population-based epidemiological study, 223 (63·5%) of which were resistant to at least one anti-tb drug, including 53 (15·1%) multidrug-resistant (mdr) isolates. spoligotyping found 243 isolates (69·2%) that belonged to the beijing family. a major subgroup of th ... | 2011 | 20429968 |
| molecular modeling studies of fatty acyl-coa synthetase (fadd13) from mycobacterium tuberculosis--a potential target for the development of antitubercular drugs. | tuberculosis (tb) is a global health problem and the situation has become more precarious due to the advent of hiv infections and continuous rise in the number of multi-drug resistant strains of mycobacterium tuberculosis (m. tb). biochemical studies on fatty acyl-coa synthetases (fadd13), one of the gene products of myma operon, have provided insights into the involvement of this protein in the activation of fatty acids. due to non-availability of the crystal structure of fadd13, we have employ ... | 2011 | 20454815 |
| prevalence and clinical predictors of pulmonary tuberculosis among isolated inpatients: a prospective study. | guidelines help to prevent the transmission of mycobacterium tuberculosis in healthcare settings, but may also result in the unnecessary isolation of many patients. we performed a prospective study to assess the prevalence and identify clinical predictors of culture-proven tuberculosis among inpatients isolated for suspected pulmonary tuberculosis (ptb) at our hospital. we also wished to validate a pre-existing clinical decision rule to improve our isolation policy. from august 2005 to january 2 ... | 2011 | 20459437 |
| in silico sequence analysis and molecular modeling of the three-dimensional structure of dahp synthase from pseudomonas fragi. | the shikimate pathway is involved in production of aromatic amino acids in microorganisms and plants. the enzymes of this biosynthetic pathway are a potential target for the design of antimicrobial compounds and herbicides. 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase (dahps) catalyzes the first step of the pathway. the gene encoding dahps was cloned and sequenced from pseudomonas fragi, the bacterium responsible for spoilage of milk, dairy products and meat. amino acid sequence deduced f ... | 2011 | 20517625 |
| expression of mycobacterium leprae hsp65 in tobacco and its effectiveness as an oral treatment in adjuvant-induced arthritis. | transgenic plants are able to express molecules with antigenic properties. in recent years, this has led the pharmaceutical industry to use plants as alternative systems for the production of recombinant proteins. plant-produced recombinant proteins can have important applications in therapeutics, such as in the treatment of rheumatoid arthritis (ra). in this study, the mycobacterial hsp65 protein expressed in tobacco plants was found to be effective as a treatment for adjuvant-induced arthritis ... | 2011 | 20526808 |
| role of 2-month sputum smears in predicting culture conversion in pulmonary tuberculosis. | sputum smears and culture conversion are frequently used to evaluate treatment response in pulmonary tuberculosis patients. limited data are available on the evaluation of the correlation between under-treatment sputum smear results and culture conversion. this prospective study included sputum culture-proven pulmonary tuberculosis patients at six hospitals in taiwan. at least two sets of sputum were collected at the completion of 8 weeks of tb treatment. the sensitivities and specificities of 2 ... | 2011 | 20516049 |
| mannosylated gelatin nanoparticles bearing isoniazid for effective management of tuberculosis. | the mannosylated gelatin nanoparticles (mn-gnps) were prepared for the selective delivery of an antitubercular drug, isoniazid (inh), to the alveolar macrophages. the gelatin nanoparticles (gnps) were prepared by using a two-step desolvation method and efficiently conjugated with mannose. various parameters such as particle size, polydispersity index, zeta potential, % entrapment efficiency, in vitro drug release, macrophage uptake, in vivo biodistribution, antitubercular activity and hepatotoxi ... | 2011 | 20540651 |
| cutaneous mycobacterium haemophilum infection in a kidney transplant recipient after acupuncture treatment. | mycobacterium haemophilum is a slow-growing nontuberculous mycobacterium that can cause disease in both immunocompetent and immunocompromised patients. the most common clinical presentations of infection are the appearance of suppurative and ulcerated skin nodules. for the diagnosis, samples collected from suspected cases must be processed under the appropriate conditions, because m. haemophilum requires lower incubation temperatures and iron supplementation in order to grow in culture. in this ... | 2011 | 20534038 |
| successful management of primary nontuberculous mycobacterial infection of hepatic allograft following orthotopic liver transplantation for hepatitis c. | nontuberculous mycobacteria are a rare cause of disease in solid organ and hematopoietic stem cell transplant recipients. the impact of mycobacterial infections in transplant recipients necessitates prompt diagnosis and early initiation of therapy. however, diagnosis remains difficult and there is a lack of specific recommendations for the choice of anti-mycobacterial drugs, duration of therapy, and monitoring of graft function as well as immunosuppression in these patients. issues involved in t ... | 2011 | 20534034 |
| pott's disease with scrofuloderma and psoas abscess misdiagnosed and treated as hidradenitis suppurativa. | a 29-year-old man with painless ulcers on the lumbar and inguinal regions associated with purulent discharge of 1.5 years' duration presented to our outpatient clinic. dermatological examination revealed palpable nodules, discharging sinuses and scars on the left lumbar, gluteal and inguinal regions. according to the clinical, histopathological, scintigraphy, and magnetic resonance imaging findings as well as mycobacteriological examinations, the patient was diagnosed with pott's disease with sc ... | 2011 | 20536275 |
| influence of bacteria on spinal implant-centered infection: an in vitro and in vivo experimental comparison between staphylococcus aureus and mycobacterium tuberculosis. | an in vitro and in vivo experimental study. | 2011 | 20543766 |
| high-resolution melting facilitates mutation screening of rpsl gene associated with streptomycin resistance in mycobacterium tuberculosis. | drug resistance remains a serious threat to tuberculosis control worldwide. as one of the important first-line antitubercular agents, resistance to streptomycin (sm) and its derivatives has increased in recent years and has become one of the characteristics of extensively drug-resistant tuberculosis (xdr-tb). a novel rapid assay to screen for rpsl gene mutations associated with sm resistance in mycobacterium tuberculosis (m. tuberculosis), was developed using high-resolution melting (hrm) analys ... | 2011 | 20554182 |