Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| tuberculosis among foreign-born persons, singapore, 2000-2009. | we determined the proportion of foreign-born persons with tuberculosis (tb) in singapore. this proportion increased from 25.5% in 2004 to 37.6% in 2009. unskilled workers from countries with high incidences of tb accounted for the highest number of and greatest increase in foreign-born tb case-patients. | 2011 | 21392448 |
| molecular epidemiology of mycobacterium tuberculosis, buenos aires, argentina. | to analyze the molecular epidemiology of mycobacterium tuberculosis strains at a hospital in buenos aires, argentina, and mutations related to multidrug-resistant and extensively drug-resistant tuberculosis, we conducted a prospective case-control study. our findings reinforce the value of incorporating already standardized molecular methods for rapidly detecting resistance. | 2011 | 21392451 |
| mycobacterium caprae infection in livestock and wildlife, spain. | mycobacterium caprae is a pathogen that can infect animals and humans. to better understand the epidemiology of m. caprae, we spoligotyped 791 animal isolates. results suggest infection is widespread in spain, affecting 6 domestic and wild animal species. the epidemiology is driven by infections in caprids, although the organism has emerged in cattle. | 2011 | 21392452 |
| drug tolerance in replicating mycobacteria mediated by a macrophage-induced efflux mechanism. | treatment of tuberculosis, a complex granulomatous disease, requires long-term multidrug therapy to overcome tolerance, an epigenetic drug resistance that is widely attributed to nonreplicating bacterial subpopulations. here, we deploy mycobacterium marinum-infected zebrafish larvae for in vivo characterization of antitubercular drug activity and tolerance. we describe the existence of multidrug-tolerant organisms that arise within days of infection, are enriched in the replicating intracellular ... | 2011 | 21376383 |
| synthesis, antibacterial and antimycobacterial activities of some new 4-aryl/heteroaryl-2,6-dimethyl-3,5-bis-n-(aryl)-carbamoyl-1,4-dihydropyridines. | a novel class of 4-aryl/heteroaryl-2,6-dimethyl-3,5-bis-n-(phenyl/substituted phenyl)-carbamoyl-1,4-dihydropyridines has been synthesized by simple, economical and eco-friendly, modified hantzsch condensation reaction making use of n-arylacetoacetamides, aryl or heteroaryl aldehydes and ammonium acetate. the newly synthesized compounds were characterized by their spectral (ir, 1h nmr, mass), elemental analyses data and evaluated for in vitro antitubercular activity against mycobacterium tubercul ... | 2011 | 21382653 |
| molecular dynamics simulations of protein targets identified in mycobacterium tuberculosis. | application of molecular dynamics simulation technique has become a conventional computational methodology to calculate significant processes at the molecular level. this computational methodology is particularly useful for analyzing the dynamics of protein-ligand systems. several uses of molecular dynamics simulation makes possible evaluation of important structural features found at interface between a ligand and a protein, such as intermolecular hydrogen bonds, contact area and binding energy ... | 2011 | 21366529 |
| bio-inspired algorithms applied to molecular docking simulations. | nature as a source of inspiration has been shown to have a great beneficial impact on the development of new computational methodologies. in this scenario, analyses of the interactions between a protein target and a ligand can be simulated by biologically inspired algorithms (bias). these algorithms mimic biological systems to create new paradigms for computation, such as neural networks, evolutionary computing, and swarm intelligence. this review provides a description of the main concepts behi ... | 2011 | 21366530 |
| the methylerythritol phosphate (mep) pathway for isoprenoid biosynthesis as a target for the development of new drugs against tuberculosis. | tuberculosis remains a major infectious disease to humans. it accounts for approximately 8-9 million new cases worldwide and an estimated 1.6 million deaths annually. effective treatments for tuberculosis consist of a combination of several drugs administered over long periods of time. since mycobacterium tuberculosis often acquires multiple drug resistant mechanisms, development of new drugs with innovative actions is urgently required. the 2c-methyl-d-erythritol 4-phosphate (mep) pathway, in c ... | 2011 | 21366531 |
| understanding the structure, activity and inhibition of chorismate synthase from mycobacterium tuberculosis. | tuberculosis is considered a worldwide health problem mainly due to co-infection with hiv and proliferation of multi-drug-resistant strains. the enzymes of the shikimate pathway are potential targets for the development of new therapies because they are essential for bacteria, but absent from mammals. the last step in this pathway is performed by chorismate synthase (cs), which catalyzes the conversion of 5-enolpyruvylshikimate-3-phosphate (epsp) to chorismate. the aim of this article is to revi ... | 2011 | 21366532 |
| fluoroquinolone and quinazolinedione activities against wild-type and gyrase mutant strains of mycobacterium smegmatis. | quinazolinediones (diones) are fluoroquinolone-like inhibitors of bacterial gyrase and dna topoisomerase iv. to assess activity against mycobacteria, c-8-methoxy dione derivatives were compared with cognate fluoroquinolones by using cultured mycobacterium smegmatis. diones exhibited higher mic values than fluoroquinolones; however, mics for fluoroquinolone-resistant gyra mutants, normalized to the mic for wild-type cells, were lower. addition of a 3-amino group to the 2,4-dione core increased re ... | 2011 | 21383100 |
| the c-terminal domain of the arabinosyltransferase mycobacterium tuberculosis embc is a lectin-like carbohydrate binding module. | the d-arabinan-containing polymers arabinogalactan (ag) and lipoarabinomannan (lam) are essential components of the unique cell envelope of the pathogen mycobacterium tuberculosis. biosynthesis of ag and lam involves a series of membrane-embedded arabinofuranosyl (araf) transferases whose structures are largely uncharacterised, despite the fact that several of them are pharmacological targets of ethambutol, a frontline drug in tuberculosis therapy. herein, we present the crystal structure of the ... | 2011 | 21383969 |
| detection and management of latent tuberculosis in liver transplant patients. | the optimal means for detecting and managing liver transplantation (lt) patients with latent tuberculosis (tb) are not well defined. our study aims were to (1) determine the frequency and risk factors of latent tb in a large cohort of consecutive adult lt candidates and (2) determine the safety and efficacy of isoniazid treatment in lt recipients with latent tb. a review of patients assessed for latent tb by skin testing using purified protein derivative (ppd; january 2004 to september 2008) or ... | 2011 | 21384513 |
| susceptibility and mode of binding of the mycobacterium tuberculosis cysteinyl transferase mycothiol ligase to trna synthetase inhibitors. | the cysteinyl transferase mycothiol ligase, or mshc, catalyzes the fourth step in the biosynthesis of the small molecular weight thiol mycothiol. mshc is essential for growth of mycobacterium tuberculosis. two groups of known aminoacyl trna synthetase inhibitors were evaluated for inhibition of m. tuberculosis mshc including aminoacyl adenosine analogs and natural products. using enzyme assays, isothermal titration calorimetry and nmr, we show that mshc is selectively inhibited by cysteinyl sulf ... | 2011 | 21392992 |
| discriminating between latent and active tuberculosis with multiple biomarker responses. | we sought to identify biomarker responses to tuberculosis specific antigens which could 1) improve the diagnosis of tuberculosis infection and 2) allow the differentiation of active and latent infections. seventy subjects with active tuberculosis (n = 12), latent tuberculosis (n = 32), or no evidence of tuberculosis infection (n = 26) were evaluated. we used the luminex multiplexed bead array platform to simultaneously evaluate 25 biomarkers in the supernatant of whole blood samples following ov ... | 2011 | 21393062 |
| adult native septic arthritis: a review of 10 years of experience and lessons for empirical antibiotic therapy. | to review the epidemiology of native septic arthritis to establish local guidelines for empirical antibiotic therapy as part of an antibiotic stewardship programme. | 2011 | 21393124 |
| mutations outside the rifampicin resistance-determining region associated with rifampicin resistance in mycobacterium tuberculosis. | ninety-six percent of rifampicin resistance in mycobacterium tuberculosis was shown to be associated with mutations inside the 81 bp rifampicin resistance-determining region (rrdr) located in the centre of the rpob gene. the detection of rifampicin resistance by targeting the rrdr failed to match with a resistant phenotype in 4% of all cases. our study aims to identify the mutations outside the rrdr that are associated with rifampicin resistance in m. tuberculosis. | 2011 | 21393153 |
| real-time pcr for single-nucleotide polymorphism detection in the 16s rrna gene as an indicator for extensive drug resistance in mycobacterium tuberculosis. | a real-time pcr screening system was established for rapid detection of single-nucleotide polymorphisms (snps) at positions 1401, 1402 and 1484 of the 16s rrna gene of mycobacterium tuberculosis leading to resistance to amikacin, kanamycin and capreomycin. resistances to the respective drugs may indicate the presence of an extensively drug-resistant (xdr) strain of m. tuberculosis. | 2011 | 21393159 |
| multicentre laboratory validation of the colorimetric redox indicator (cri) assay for the rapid detection of extensively drug-resistant (xdr) mycobacterium tuberculosis. | to perform a multicentre study to evaluate the performance of the colorimetric redox indicator (cri) assay and to establish the mics and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin. | 2011 | 21393176 |
| rapid detection of resistance to pyrazinamide in mycobacterium tuberculosis using the resazurin microtitre assay. | the resazurin microtitre plate assay (rema) was evaluated to determine the susceptibility of mycobacterium tuberculosis to pyrazinamide, and was compared with the broth microdilution method (bmm), the absolute concentration method (acm) and pyrazinamidase (pzase) determination. | 2011 | 21393185 |
| evaluation of a rapid detection method of clarithromycin resistance genes in mycobacterium avium complex isolates. | clarithromycin is the key drug in the various treatment regimens of mycobacterium avium complex (mac) diseases, and is the only drug for which drug susceptibility has been shown to correlate with clinical response in these diseases. a point mutation at either positions 2058 or 2059 of the 23s rrna gene has been reported to occur in high-level clarithromycin-resistant isolates. in this study, we examined the correlation between the results from a drug susceptibility test and the mutation of the 2 ... | 2011 | 21393190 |
| environmental risk factors for pulmonary mycobacterium avium-intracellulare complex disease. | mycobacterium avium-intracellulare complex (mac) is a ubiquitous pathogen found in soil and water. environmental exposure is the primary route for mac infection. however, specific environmental risk factors have been poorly determined in immunocompetent patients with pulmonary mac disease. | 2011 | 21393389 |
| cloning, expression, purification, crystallization and preliminary x-ray diffraction analysis of the regulatory domain of aspartokinase (rv3709c) from mycobacterium tuberculosis. | the regulatory domain of mycobacterium tuberculosis aspartokinase (mtb-ak, mtb-ask, rv3709c) has been cloned, heterologously expressed in escherichia coli and purified using standard chromatographic techniques. screening for initial crystallization conditions using the regulatory domain (ak-+¦) in the presence of the potential feedback inhibitor threonine identified four conditions which yielded crystals suitable for x-ray diffraction analysis. from these four conditions five different crystal f ... | 2011 | 21393848 |
| [a case of wegener granulomatosis after treatment for non-tuberculous mycobacteriosis]. | a 67-year-old woman with a history of non-tuberculous mycobacteriosis due to mycobacterium avium in 2002 and 2006 was admitted because of low-grade fever and cavitary lesions in both lungs on chest computed tomography (ct). bronchial brushing cytology by bronchoscopy did not yield mycobacterium. her serum antineutrophil cytoplasmic antibodies (mpo-anca) level was elevated and pr3 anca was negative. a surgical lung biopsy revealed basophilic necrosis with cavitary lesions and necrotizing vasculit ... | 2011 | 21384680 |
| knowledge of cervical tuberculosis lymphadenitis and its treatment in pastoral communities of the afar region, ethiopia. | infection with mycobacterium bovis (mb) predominantly causes cervical tb lymphadenitis (tbl). raw milk is considered the main source of mb infection and raw milk is a major food source for afar pastoralists. the aim of this study was to assess afar pastoralists' knowledge concerning cervical tbl and its treatment. | 2011 | 21385472 |
| paratuberculosis in european wild rabbits from the iberian peninsula. | of the non-ruminant wildlife species known to harbor mycobacterium avium paratuberculosis (map), the rabbit (oryctolagus cuniculus) is thought to pose the greatest risk of transmission to cattle. we analyzed 80 hunter-harvested wild rabbits from a core study area in southern spain, and sera from 157 wild rabbits sampled opportunistically on seven additional sites. gross lesions compatible with paratuberculosis were observed in two of 80 necropsied rabbits. histopathology revealed focal to diffus ... | 2011 | 21367439 |
| molecular characterization of isoniazid-resistant mycobacterium tuberculosis isolates from xi'an, china. | the increased prevalence of isoniazid (inh)-resistant tuberculosis (tb) has become a significant challenge for tb control in the last 10 years. it has been reported that inh resistance is most frequently associated with katg 315 and inha-15 mutations. the relative significance of these mutations in inh-resistant mycobacterium tuberculosis and their association with the different genotypes of m. tuberculosis in xi'an, china, was investigated. eighty-nine inh-resistant isolates were subjected to m ... | 2011 | 21388297 |
| new multistage tb vaccine shown to produce stronger, longer-lasting response against mycobacterium tuberculosis. | 2011 | 21395372 | |
| discovery of novel methanone derivatives acting as antimycobacterial agents. | a series of pyrazoline derivatives were synthesized and in vitro activity against mycobacterium tuberculosis h37rv was carried out. among the synthesized compounds, compounds (4d) and (4f) 4-aminophenyl-3-(3,4-dimethoxyphenyl)-6,7-dimethoxy-2,3,3a,4-tetrahydroindeno[1,2-c]pyrazol-2-ylmethanone and 4-aminophenyl-6,7-dimethoxy-3-phenyl-2,3,3a,4-tetrahydroindeno[1,2-c]pyrazol-2-ylmethanone were found to be the most active agent against m. tuberculosis h37rv with a minimum inhibitory concentration o ... | 2011 | 21395486 |
| a review of molecular modelling studies of dihydrofolate reductase inhibitors against opportunistic microorganisms and comprehensive evaluation of new models. | dihydrofolate reductase (dhfr) has been used as a target for antimicrobial drug discovery against a variety of pathogenic microorganisms, including opportunistic microorganisms; pneumocystis carinii (pc), toxoplasma gondii (tg) and mycobacterium avium complex (ma). in this regard, several dhfr inhibitors are reported against pc and tg and ma. however, selectivity issue of these inhibitors over human dhfr often preclude their development and clinical use. in the first part of this work, various c ... | 2011 | 21395543 |
| development of multiplex pcr assays based on the 16s-23s rrna internal transcribed spacer for the detection of clinically relevant nontuberculous mycobacteria. | to accelerate the identification and differentiation of clinically relevant nontuberculous mycobacteria (ntm) with two sets of multiplex pcr (mpcr) targeting the 16s-23s rrna internal transcribed spacer (its) region for timely patient management. | 2011 | 21395629 |
| crystal structure of calcium dodecin (rv0379), from mycobacterium tuberculosis with a unique calcium-binding site. | in eukaryotes, calcium-binding proteins play a pivotal role in diverse cellular processes, and recent findings suggest similar roles for bacterial proteins at different stages in their life cycle. here, we report the crystal structure of calcium dodecin, rv0379, from mycobacterium tuberculosis with a dodecameric oligomeric assembly and a unique calcium-binding motif. structure and sequence analysis were used to identify orthologs of rv0379 with different ligand-binding specificity. | 2011 | 21370306 |
| mycobacterium tuberculosis infection of end-stage renal disease patients in taiwan: a nationwide longitudinal study. | clin microbiol infect abstract: end-stage renal disease (esrd) patients are vulnerable to mycobacterium tuberculosis (mtb) infection, but the magnitude of the risk is uncertain. in addition, there is no reliable information on the mtb infection rate of patients undergoing different types of renal replacement therapy (rrt). we used the taiwan national health insurance research database to conduct a 9-year nationwide longitudinal study. among 49 983 esrd patients who received three renal replaceme ... | 2011 | 21375664 |
| a modified scoring system to describe gross pathology in the rabbit model of tuberculosis. | the rabbit model is an ideal means to study the pathogenesis of tuberculosis due to its semblance to the disease in humans. we have previously described the results using a bronchoscopic route of infection with live bacilli as a reliable means of generating lung cavities in sensitized rabbits. the role of sensitization in the development of disease outcomes has been well established in several animal models. we have described here the varying gross pathology that result from lack of sensitizatio ... | 2011 | 21375756 |
| mycobacterium avium subsp. paratuberculosis in dairy products, meat, and drinking water. | mycobacterium avium subsp. paratuberculosis (map) is the cause of johne's disease, a chronic infection of the gut, in ruminant animals that provide milk and/or meat for human consumption. map also may be involved in crohn's disease and type 1 diabetes in humans. although the role of map in human diseases has not been established, minimizing the exposure of humans to the organism is considered desirable as a precautionary measure. infected animals can shed map in feces and milk, and the organism ... | 2011 | 21375889 |
| host-pathogen interactions made transparent with the zebrafish model. | the zebrafish holds much promise as a high-throughput drug screening model for immune-related diseases, including inflammatory and infectious diseases and cancer. this is due to the excellent possibilities for in vivo imaging in combination with advanced tools for genomic and large scale mutant analysis. the context of the embryo's developing immune system makes it possible to study the contribution of different immune cell types to disease progression. furthermore, due to the temporal separatio ... | 2011 | 21366518 |
| design, synthesis and antimycobacterial activity of cinnamide derivatives: a molecular hybridization approach. | a series of cinnamide derivatives was designed as potential antimycobacterial agents using molecular hybridization approach. the diamine moiety, a key feature of ethambutol and its other analogs, and certain structural features of cerulenin and cinnamic acid were hybridized to obtain cinnamide derivatives. the minimum inhibitory concentration (mic) of all synthesized compounds was determined against m. tuberculosis h(37)r(v) using resazurin microtitre plate assay (rema) method. the synthesized m ... | 2011 | 21376586 |
| novel thiolactone-isatin hybrids as potential antimalarial and antitubercular agents. | the synthesis of a novel series of thiolactone-isatin hybrids led to the discovery of tetracyclic by-products which displayed superior antiplasmodial activity. the tetracycles thus formed the basis of a more focused sar study. identified from this series is a compound with an ic(50) of 6.92 µm against the chloroquine-resistant (w2) strain of plasmodium falciparum. useful antimalarial sars delineated include the need for substitution at c-5 of the isatin scaffold and the enhancement of activity b ... | 2011 | 21376591 |
| immunopathological characteristics of immune reconstitution inflammatory syndrome caused by mycobacterium parascrofulaceum infection in a patient with aids. | immune reconstitution inflammatory syndrome (iris) caused by mycobacterium in patients with aids is often experienced in clinical practice. there is, however, a paucity of data documenting the histopathological findings and the pathogenesis. we determined the immunopathological characteristics of iris associated with mycobacterium parascrofulaceum infection in an aids patient. a patient presented with pulmonary lymphadenitis and involvement of the pulmonary lingular segment. portions of the invo ... | 2011 | 21377277 |
| microbial metabolism of a-mangostin isolated from garcinia mangostana l. | a-mangostin (1), a prenylated xanthone isolated from the fruit hull of garcinia mangostana l., was individually metabolized by two fungi, colletotrichum gloeosporioides (eyl131) and neosartorya spathulata (eyr042), repectively. incubation of 1 with c. gloeosporioides (eyl131) gave four metabolites which were identified as mangostin 3-sulfate (2), mangostanin 6-sulfate (3), 17,18-dihydroxymangostanin 6-sulfate (4)and isomangostanin 3-sulfate (5). compound 2 was also formed by incubation with n. s ... | 2011 | 21377704 |
| identification of a glycosyltransferase from mycobacterium marinum involved in addition of a caryophyllose moiety in lipooligosaccharides. | deletion of mycobacterium marinum mmar2333 resulted in the loss of three of four subclasses of lipooligosaccharides (loss). the mutant was unable to extend an intermediate (los-ii*) by addition of caryophyllose. these data and the predicted domain structure suggest that mmar2333 is a glycosyltransferase involved in the generation of a lipid-linked caryophyllose donor. | 2011 | 21378187 |
| mycobacterial factors relevant for transmission of tuberculosis. | tuberculosis (tb) transmission is associated with patient-related risk factors. however, dna fingerprint analysis has provided anecdotal evidence suggesting a role for bacteriological factors. | 2011 | 21378376 |
| fourth ventricle tuberculoma. | 2011 | 21378432 | |
| mycobacterial infections in the era of modern biologic agents. | patients with rheumatologic disease are at increased risk for mycobacterial infections because of both immunocompromising therapy and preexisting lung damage from the disease itself. tuberculosis is uncommon in the united states; however, it is relatively more prevalent in immigrants, minorities, elderly, persons with acquired immune deficiency syndrome and among healthcare workers. in the united states, the nontuberculous mycobacteria (ntm) are now more prevalent than tuberculosis. although ini ... | 2011 | 21378550 |
| meropenem/clavulanate and linezolid treatment for extensively drug-resistant tuberculosis. | the combination of meropenem with clavulanate has high antimycobacterial activity in vitro against extensively drug-resistant mycobacterium tuberculosis strains. we report the successful use of this combination in association with linezolid in the management of an advanced extensively drug-resistant tuberculosis disease with complex second-line drug resistance in a 14-year-old teenager. | 2011 | 21378593 |
| uptake of unnatural trehalose analogs as a reporter for mycobacterium tuberculosis. | the detection of tuberculosis currently relies upon insensitive and unspecific techniques; newer diagnostics would ideally co-opt specific bacterial processes to provide real-time readouts. the trehalose mycolyltransesterase enzymes (antigens 85a, 85b and 85c (ag85a, ag85b, ag85c)) serve as essential mediators of cell envelope function and biogenesis in mycobacterium tuberculosis. through the construction of a systematically varied sugar library, we show here that ag85 enzymes have exceptionally ... | 2011 | 21378984 |
| a mycobacterium marinum tesa mutant defective for major cell wall-associated lipids is highly attenuated in dictyostelium discoideum and zebrafish embryos. | infection of the zebrafish with mycobacterium marinum is regarded as a well-established experimental model to study the pathogenicity of mycobacterium tuberculosis. herein, a m. marinum transposon mutant library was screened for attenuated m. marinum phenotypes using a dictyostelium discoideum assay. in one attenuated mutant, the transposon was located within tesa, encoding a putative type ii thioesterase. thin-layer chromatography analyses indicated that the tesa::tn mutant failed to produce tw ... | 2011 | 21375593 |
| mycobacteria release active membrane vesicles that modulate immune responses in a tlr2-dependent manner in mice. | bacteria naturally release membrane vesicles (mvs) under a variety of growth environments. their production is associated with virulence due to their capacity to concentrate toxins and immunomodulatory molecules. in this report, we show that the 2 medically important species of mycobacteria, mycobacterium tuberculosis and mycobacterium bovis bacille calmette-guérin, release mvs when growing in both liquid culture and within murine phagocytic cells in vitro and in vivo. we documented mv productio ... | 2011 | 21364279 |
| tuberculosis transmission across the united states-mexico border. | in this era of increasing drug resistance among infectious diseases such as tuberculosis (tb), the complex population dynamics of border areas must be monitored more extensively. tb remains a major public health threat; its antimicrobial treatment is long; and the only vaccine licensed in the world, live-attenuated mycobacterium bovis bacille calmette-gu+®rin (bcg), exhibits varying efficacy. in addition to epidemiological surveillance, the underlying determinants contributing to the health and ... | 2011 | 21390421 |
| detection of tuberculous lymphadenopathy by positron emission tomography/computed tomography in a patient with behçet's disease. | 2011 | 21373744 | |
| mycobacterium avium and modulation of the host macrophage immune mechanisms. | pathogenesis by mycobacteria requires the exploitation of host-cell signaling pathways to enhance intracellular survival and persistence of the pathogen. among patients with end-stage acquired immune-deficiency syndrome, disseminated infection with mycobacterium avium, a member of the m. avium complex (mac), is the most common bacterial infection. the virulence and intrinsic multidrug resistance of this pathogen has been attributed in part to its unique cell wall, which is a complex array of hyd ... | 2011 | 21396201 |
| yield of culture of mycobacterium tuberculosis complex in sputum samples transported from tribal areas. | there is little information on the recovery of mycobacterium tuberculosis complex from sputum samples collected in the field for bacteriological examination. | 2011 | 21396206 |
| evaluation of light emitting diode-based fluorescence microscopy for the detection of mycobacteria in a tuberculosis-endemic region. | to evaluate fluorescence microscopy (fm) using light emitting diode (led) technology for the detection of acid-fast bacilli at a tertiary referral centre in mumbai, india, a tuberculosis-endemic country. | 2011 | 21396207 |
| drug resistance among tuberculosis patients attending diagnostic and treatment centres in makassar, indonesia. | government tuberculosis (tb) diagnostic and treatment centres, makassar, indonesia. | 2011 | 21396208 |
| rapid detection of multidrug-resistant mycobacterium tuberculosis by multiplex allele-specific polymerase chain reaction. | dr cetrangolo hospital, buenos aires province, argentina. | 2011 | 21396209 |
| wild-type distributions of seven oral second-line drugs against mycobacterium tuberculosis. | to determine wild-type minimum inhibitory concentration (mic) distributions for mycobacterium tuberculosis, as the background data for defining susceptibility breakpoints are limited. | 2011 | 21396210 |
| molecular genotyping of mycobacterium tuberculosis in xi'an, china, using miru-vntr typing. | the molecular genotyping of mycobacterium tuberculosis is expected to lead to a better understanding of m. tuberculosis transmission in xi'an, one of the largest cities in western china. | 2011 | 21396212 |
| resistance patterns of multidrug-resistant tuberculosis in western province, papua new guinea. | few data are available on tuberculosis (tb) drug resistance patterns in papua new guinea (png) due to the lack of facilities for mycobacterial culture. many patients from the western province seek care in queensland health clinics in the torres strait. since 2000, we have treated 161 tb cases from png, of whom 40 proved to have multidrug-resistant tb (mdr-tb; two human immunodeficiency virus positive). drug susceptibility testing (dst) shows high levels of resistance to other drugs in the mdr-tb ... | 2011 | 21396218 |
| rapid and efficient detection of mycobacterium tuberculosis in respiratory and non-respiratory samples. | the detection of mycobacterium tuberculosis using a new commercial real-time polymerase chain reaction (pcr) assay (xpertôäó mtb/rif) was evaluated on 91 respiratory and 89 non-respiratory samples recovered from 132 patients suspected of tuberculosis (tb). overall, 31 (17.2%) of the 180 samples, including 17 respiratory and 14 non-respiratory (respectively 17 and 12 pcr-positive), yielded m. tuberculosis on culture. the sensitivity and specificity of pcr were respectively 100% and 100%, and 85.7 ... | 2011 | 21396219 |
| characterization of a newly identified mycobacterial tautomerase with promiscuous dehalogenase and hydratase activities reveals a functional link to a recently diverged cis-3-chloroacrylic acid dehalogenase. | the enzyme cis-3-chloroacrylic acid dehalogenase (cis-caad) is found in a bacterial pathway that degrades a synthetic nematocide, cis-1,3-dichloropropene, introduced in the 20th century. the previously determined crystal structure of cis-caad and its promiscuous phenylpyruvate tautomerase (ppt) activity link this dehalogenase to the tautomerase superfamily, a group of homologous proteins that are characterized by a catalytic amino-terminal proline and a ß-a-ß structural fold. the low-level ppt a ... | 2011 | 21370851 |
| membrane-active antimicrobial peptides and human placental lysosomal extracts are highly active against mycobacteria. | mycobacterium tuberculosis (mtb), the causative agent of tuberculosis, manifests discreet strategies to subvert host immune responses, which enable the pathogen to survive and multiply inside the macrophages. this problem is further worsened by the emergence of multidrug resistant mycobacterial strains, which make most of the anti-tuberculous drugs ineffective. it is thus imperative to search for and design better therapeutic strategies, including employment of new antibiotics. recently, natural ... | 2011 | 21396418 |
| evaluation of an immunochromatographic assay for rapid identification of mycobacterium tuberculosis complex in clinical isolates. | identification of mycobacterium tuberculosis complex (mtc) remains slow. over the years, several new technologies have been proposed to accelerate and simplify the detection of mtc. in this context, we evaluated an immunochromatographic assay (ica) (bio-line sd ag mpt64 tb) for rapid identification of mtc, based on detection of a specific mpt64 antigen of mtc. we have tested it on i) mycobacterial cultures: 210 mtc strains and 28 nontuberculous mycobacteria; ii) m. bovis bacille calmette-gu+®rin ... | 2011 | 21396535 |
| the ß-ketoacyl-acp synthase from mycobacterium tuberculosis as potential drug targets. | the continuous preventive measures and control of tuberculosis are often hampered by re-emergence of multi-drug-resistant (mdr) strains of mycobacterium tuberculosis. a novel drug approach is desperately needed to combat the global threat posed by mdr strains. in spite of current advancement in biological techniques viz. microarray and proteomics data for tuberculosis, no such potent drug has been developed in the past decades yet. therefore, mycolic acid is an essential constituent which is inv ... | 2011 | 21370994 |
| a group of low molecular weight (<30 kda) antigens of heat-killed mycobacterium tuberculosis h37rv confer better protection against experimental tuberculosis in guinea pigs. | heat-killed mycobacterium tuberculosis (mtb) h37rv-derived antigens were purified by electroelution, and their efficacy was evaluated in conferring protection against tb in guinea pigs in order to identify those protein antigens as a subunit vaccine. a total sonicate extract of mtb h37rv (mtse) was prepared by growing bacteria on lowenstein-jensen (lj) solid medium to its mid-log phase, harvested, and subjected to ultrasonication. fractions of mtse were resolved on 10% polyacrylamide gel electro ... | 2011 | 21391049 |
| bridging implementation, knowledge, and ambition gaps to eliminate tuberculosis in the united states and globally. | we reflect on remarkable accomplishments in global tuberculosis (tb) control and identify persistent obstacles to the successful elimination of tb from the united states and globally. one hundred and twenty nine years after koch's discovery of the etiologic agent of tb, this health scourge continues to account for 9.4 million cases and 1.7 million deaths annually worldwide. implementation of the directly observed treatment short-course strategy from 1995 through 2009 has saved 6 million lives. t ... | 2011 | 21392421 |
| nontuberculous mycobacteria in respiratory tract infections, eastern asia. | to characterize the distribution of nontuberculous mycobacteria (ntm) species isolated from pulmonary samples from persons in asia and their association with pulmonary infections, we reviewed the literature. mycobacterium avium complex bacteria were most frequently isolated (13%-81%) and were the most common cause of pulmonary ntm disease (43%-81%). also pathogenic were rapidly growing mycobacteria (m. chelonae, m. fortuitum, m. abscessus). among all ntm isolated from pulmonary samples, 31% (582 ... | 2011 | 21392422 |
| innate response of human endothelial cells infected with mycobacteria. | endothelial cells are susceptible to infection by several pathogens, but little is known about mycobacterial infection. we analyzed some features of mycobacteria-endothelial cell interactions and the innate response to the infection. intracellular growth in human umbilical vein endothelial cells (huvecs) of three mycobacterium species: m. tuberculosis (mtb), m. abscessus (mab) and m. smegmatis (msm) was analyzed. m. smegmatis was eliminated; m. abscessus had an accelerate intracellular replicati ... | 2011 | 21397978 |
| identification of novel potential antibiotics for tuberculosis by in silico structure-based drug screening. | the enoyl-acyl carrier protein reductase of mycobacterium tuberculosis (mtb) is a key enzyme of the type ii fatty acid synthesis system. it is involved in the production of mycolic acid and is a known target for isoniazid, an effective antibiotic for tuberculosis treatment. the increasing prevalence of tuberculosis in many areas of the world, which is associated with the rise of drug-resistant mtb strains, presents a major global health threat. in this study, we attempted to identify novel antib ... | 2011 | 21397998 |
| isoniazid-resistant tuberculous meningitis, united states, 1993-2005. | to determine patient characteristics associated with isoniazid resistance in cases of tuberculous meningitis, we conducted a cross-sectional study by using data from the us national tuberculosis surveillance system during 1993-2005. foreign-born patients were more likely to be infected with an isoniazid-resistant strain. | 2011 | 21392454 |
| use of the intradermal tuberculin test in a herd of captive elk (cervus elaphus nelsoni) naturally infected with mycobacterium bovis. | in the united states, tuberculosis of captive cervids, caused by mycobacterium bovis, attracted attention in 1991 when investigations, prompted by the identification of a tuberculous elk (cervus elaphus nelsoni) of u.s. origin exported to canada, revealed tuberculosis in 10 different elk herds in 8 different states. based on methods used in cattle, official regulations pertaining to testing and eradication of tuberculosis in captive cervids were added to the u.s. department of agriculture bovine ... | 2011 | 21398465 |
| persistence of mycobacterium avium subsp. paratuberculosis at a farm-scale biogas plant supplied with manure from paratuberculosis-affected dairy cattle. | in this study, products from all steps of anaerobic digestion at a farm-scale biogas plant supplied with manure from paratuberculosis-affected dairy cattle were examined and quantified for the presence of the causal agent of paratuberculosis, mycobacterium avium subsp. paratuberculosis, using culture and quantitative real-time pcr (qpcr). viable m. avium subsp. paratuberculosis cells were detected using culture in fermentors for up to 2 months; the presence of m. avium subsp. paratuberculosis dn ... | 2011 | 21398476 |
| robert koch. | 2011 | 21392456 | |
| mycobacterium novocastrense-associated pulmonary and wound infections. | 2011 | 21392457 | |
| mycobacterium avium intracellulare infection coexistent with nodular lymphocyte predominant hodgkin lymphoma involving the lung. | a 5.5-year-old asymptomatic hispanic/african american male presented with matted lymph nodes in the neck and reticulonodular opacities in the right upper lung. an extensive diagnostic work up was performed to rule out infectious etiologies. biopsies of the lymph node and lung tissue were diagnostic of nodular lymphocyte predominant hodgkin lymphoma. two weeks into the chemotherapy, gastric aspirates grew mycobacterium avium intracellulare. this is the first case of nodular lymphocyte predominant ... | 2011 | 21399527 |
| geographic expansion of buruli ulcer disease, cameroon. | 2011 | 21392458 | |
| risk for mycobacterium celatum infection from ferret. | 2011 | 21392459 | |
| acid-fast staining and petroff-hausser chamber counting of mycobacterial cells in liquid suspension. | accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. here, a method using modified kinyoun acid-fast staining was adapted for use with a petroff-hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation. | 2011 | 21400674 |
| mycobacterium mageritense pulmonary disease in patient with compromised immune system. | 2011 | 21392461 | |
| extensively drug-resistant tuberculosis, china. | 2011 | 21392462 | |
| diagnosis and treatment of tuberculosis in the private sector, vietnam. | 2011 | 21392464 | |
| fluoroquinolone-resistant mycobacterium tuberculosis, pakistan, 2005-2009. | 2011 | 21392465 | |
| mycobacterium leprae in ocular tissues: histopathological findings in experimental leprosy. | 2011 | 21393973 | |
| extremely high prevalence of multidrug resistant tuberculosis in murmansk, russia: a population-based study. | drug resistance and molecular epidemiology of tuberculosis (tb) in the murmansk region was investigated in a 2-year, population-based surveillance of the civilian population. during 2003 and 2004, isolates from all culture-positive cases were collected (nôçë=ôçë1,226). prevalence of multi-drug resistance (mdr) was extremely high, as 114 out of 439 new cases (26.0%), and 574 out of 787 previously treated cases (72.9%) were resistant to at least isoniazid (inh) and rifampin (rif). spoligotyping of ... | 2011 | 21394425 |
| aprabc: a mycobacterium tuberculosis complex-specific locus that modulates ph-driven adaptation to the macrophage phagosome. | following phagocytosis by macrophages, mycobacterium tuberculosis (mtb) senses the intracellular environment and remodels its gene expression for growth in the phagosome. we have identified an acid and phagosome regulated (aprabc) locus that is unique to the mtb complex and whose gene expression is induced during growth in acidic environments in vitro and in macrophages. using the apra promoter, we generated a strain that exhibits high levels of inducible fluorescence in response to growth in ac ... | 2011 | 21401735 |
| b cells can modulate the cd8 memory t cell after dna vaccination against experimental tuberculosis. | 2011 | 21401938 | |
| new s-benzylisothiosemicarbazones with antimycobacterial activity. | benzaldehyde- and salicylaldehyde-s-benzylisothiosemicarbazones show a moderate to high in vitro activity against mycobacterium tuberculosis, mycobacterium avium and two strains of mycobacterium kansasii. benzaldehyde-s-4-bromobenzylisothiosemicarbazone and salicylaldehyde-s-4-chlorobenzylisothiosemicarbazone have the most promising antimycobacterial properties. | 2011 | 21394476 |
| bioactive metabolites of diaporthe sp. p133, an endophytic fungus isolated from pandanus amaryllifolius. | two new benzopyranones, diaportheone a (1) and b (2), were obtained via bioassay-guided isolation of the secondary metabolites from the endophytic fungus diaporthe sp. p133 isolated from pandanus amaryllifolius leaves. their structures were determined from spectral analysis including mass spectrometry, 1d and 2d nuclear magnetic resonance. both 1 and 2 inhibited the growth of the virulent strain of mycobacterium tuberculosis h(37)rv with minimum inhibitory concentrations of 100.9 and 3.5-á++m, r ... | 2011 | 21394664 |
| activity of carbapenems combined with clavulanate against murine tuberculosis. | although beta-lactam antibiotics are not considered as antituberculous drugs, it has been recently shown that the combination of carbapenems and clavulanate is bactericidal in vitro. we evaluated in a murine model of tuberculosis the activity of carbapenems alone and combined with clavulanate against mycobacterium tuberculosis. swiss mice infected intravenously with 3 x 105 m. tuberculosis h37rv were treated for 4 weeks with clavulanate alone or imipenem, meropenem, and ertapenem alone or combin ... | 2011 | 21402832 |
| interferon-gamma release assay in tuberculous scleritis. | 2011 | 21402999 | |
| relationship between single nucleotide polymorphism of interleukin-18 and susceptibility to pulmonary tuberculosis in the chinese han population. | interleukin-18 (il-18) is a multi-functional cytokine capable of inducing either th1 or th2 polarization depending on the immunologic milieu. il-18 may influence the host response to mycobacterium tuberculosis (m.tb) infection. to investigate the relationship between single nucleotide polymorphisms of the il-18 and susceptibility to pulmonary tuberculosis in the chinese han population, the il-18 gene was sequenced to detect polymorphisms and to examine the genotype frequencies in 300 patients an ... | 2011 | 21395662 |
| cloning, expression, and immunogenicity of novel fusion protein of mycobacterium tuberculosis based on esat-6 and truncated c-terminal fragment of hsp70. | tuberculosis (tb) remains as a major public health problem worldwide. identification and selection of immunodominant antigens of mycobacterium tuberculosis (mtb), capable of efficiently inducing a protective immune response is the ultimate goal of tb vaccine development studies. accordingly, this study was designed to produce a novel m. tuberculosis fusion protein consisted of mtb esat-6 (early secreted antigenic target-6-ákda), as a potent immunogenic protein, fused to c-terminus of mtb hsp70 ( ... | 2011 | 21388826 |
| a high-throughput screen for tuberculosis progression. | one-third of the world population is infected with mycobacterium tuberculosis and multi-drug resistant strains are rapidly evolving. the noticeable absence of a whole organism high-throughput screening system for studying the progression of tuberculosis is fast becoming the bottleneck in tuberculosis research. we successfully developed such a system using the zebrafish mycobacterium marinum infection model, which is a well-characterized model for tuberculosis progression with biomedical signific ... | 2011 | 21390204 |
| diversity in functional organization of class i and class ii biotin protein ligase. | the cell envelope of mycobacterium tuberculosis (m. tuberculosis) is composed of a variety of lipids including mycolic acids, sulpholipids, lipoarabinomannans, etc., which impart rigidity crucial for its survival and pathogenesis. acyl coa carboxylase (acc) provides malonyl-coa and methylmalonyl-coa, committed precursors for fatty acid and essential for mycolic acid synthesis respectively. biotin protein ligase (bpl/bira) activates apo-biotin carboxyl carrier protein (bccp) by biotinylating it t ... | 2011 | 21390227 |
| the mechanisms of hamp-mediated signaling in transmembrane receptors. | hamp domains mediate signal transduction in over 7500 enzyme-coupled receptors represented in all kingdoms of life. the hamp domain of the putative archaeal receptor af1503 has a parallel, dimeric, four-helical coiled coil structure, but with unusual core packing, related to canonical packing by concerted axial rotation of the helices. this has led to the gearbox model for signal transduction, whereby the alternate packing modes correspond to signaling states. here we present structures of a ser ... | 2011 | 21397188 |
| assessment of the sd bioline ag mpt64 rapidôäó and the mgitôäó tbc identification tests for the diagnosis of tuberculosis. | successful control of tuberculosis relies on the rapid detection of mycobacterium tuberculosis. few chromatographic lateral flow assays for the discrimination of the m. tuberculosis complex were developed from culture media. we compared the values of 2 assays to assess their place in diagnosis of tuberculosis. we conclude of their efficiency and relevance to supplant the conventional methods. | 2011 | 21397427 |
| tuberculosis at the human-animal interface: an emerging disease of elephants. | over the past 15 years, cases of infection with organisms of the mycobacterium tuberculosis complex have been diagnosed among captive elephants in the united states and worldwide. outbreak investigations have documented that among staff employed at facilities housing infected animals, skin test conversion to purified protein derivative have been documented. clonal spread among animals in close contact and even inter-species spread between elephant and human has been documented. detection of acti ... | 2011 | 21397564 |
| computational tools to study and understand the intricate biology of mycobacteria. | the field of mycobacteriology is currently an area of intense research. to deal with the copious amount of data being generated, numerous web servers and databases have been developed. however, these are available at disparate sites and there exists no single source/platform which provides information about their utility and access. therefore, a comprehensive compilation of various bioinformatics tools/resources dedicated to mycobacteria is presented in this article. | 2011 | 21398182 |
| [a case having chyliform pleural effusion caused by former tuberculous pleurisy]. | a 49-year-old male who had been treated for pulmonary tuberculosis and tuberculous pleurisy in 2007 was referred to our hospital with the complaint of dyspnea on exertion in nov. 2009. chest x-ray showed increased pleural effusion compared with that remaining after the previous treatment of pleurisy in 2008. a chest ct revealed that fluid collection was surrounded by thickened pleura. thoracocentesis was performed, and yellow milky liquid was obtained. the pleural effusion contained few cells. t ... | 2011 | 21404651 |
| [genetic research about mycobacterium avium complex]. | a variable-number tandem-repeat (vntr) typing method using the mycobacterium avium tandem repeat (matr) loci (matr-vntr) is employed in japan for epidemiological studies using clinical isolates of m. avium. in this study, the usefulness of this matr-vntr typing method was compared with that of the is1245-restriction fragment length polymorphism (is1245-rflp) typing method and a mycobacterial interspersed repetitive-unit (miru)-vntr typing method reported previously (boschiroli, c. hubbans, p. ov ... | 2011 | 21404652 |
| [non-tuberculous mycobacteriosis. what has been coming out]. | diagnosis of non-tuberculous mycobacteriosis is relatively easy, because of recent technological advances (hrct, mgit, pcr, ddh etc). although many reports of this disease have been published, there are many problems to resolve. (1) prevalence of non-tuberculous mycobacteriosis: shigeki sato (department of medical oncology and immunology, nagoya city university graduate school of medical sciences) questionnaire surveys to determine the prevalence of nontuberculous mycobacterial (ntm) disease wer ... | 2011 | 21404655 |
| diabetes and tuberculosis: analysis of a paradox. | diabetes mellitus is a known predisposition factor for tuberculosis. however, the cause of such interrelationship is incompletely understood. diabetes mellitus is characterized by hyperglycemia. interestingly, glucose has been shown to stimulate nadph oxidase, the key enzyme involved with respiratory burst in monocytes and macrophages. this glucose-induced genesis of reactive oxygen species via respiratory burst is contrary to the survival strategy of mycobacterium tuberculosis as an intracellul ... | 2011 | 21404917 |