Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| modeling metabolic networks in c. glutamicum: a comparison of rate laws in combination with various parameter optimization strategies. | to understand the dynamic behavior of cellular systems, mathematical modeling is often necessary and comprises three steps: (1) experimental measurement of participating molecules, (2) assignment of rate laws to each reaction, and (3) parameter calibration with respect to the measurements. in each of these steps the modeler is confronted with a plethora of alternative approaches, e. g., the selection of approximative rate laws in step two as specific equations are often unknown, or the choice of ... | 2009 | 19144170 |
| antimicrobial activity of some water plants from the northeastern anatolian region of turkey. | the antimicrobial activity of methanol and acetone extracts of butomus umbellatus, polygonum amphibium, and two species of the genus sparganium (s. erectum and s. emersum)against three gram-positive, five gram-negative bacteria and one fungus was assessed by the disk diffusion method. the microorganisms used were staphylococcus aureusatcc-29740, escherichia coli atcc-25922, pseudomonas aeruginosa atcc-15442, salmonella typhi nctc-9394, klebsiella pneumoniae nctc-5046, proteus vulgaris atcc-7829, ... | 2009 | 19145212 |
| development and use of a selectable, broad-host-range reporter transposon for identifying environmentally regulated promoters in bacteria. | this report describes the development and use of tnknxsp, a selectable broad-host-range reporter transposon with a promoterless apha gene. insertion of tnknxsp into the chromosome of a kanamycin-susceptible bacterium confers resistance to kanamycin only if apha is transcribed from an active promoter adjacent to the insertion site. we designed tnknxsp as a tool for identifying environmentally regulated promoters in bacteria and developed general methods for initial characterization of any tnknxsp ... | 2009 | 19146571 |
| reliable transfer of transcriptional gene regulatory networks between taxonomically related organisms. | transcriptional regulation of gene activity is essential for any living organism. transcription factors therefore recognize specific binding sites within the dna to regulate the expression of particular target genes. the genome-scale reconstruction of the emerging regulatory networks is important for biotechnology and human medicine but cost-intensive, time-consuming, and impossible to perform for any species separately. by using bioinformatics methods one can partially transfer networks from we ... | 2009 | 19146695 |
| gender-specific differences between the concentrations of nonvolatile (r)/(s)-3-methyl-3-sulfanylhexan-1-ol and (r)/(s)-3-hydroxy-3-methyl-hexanoic acid odor precursors in axillary secretions. | the volatile fatty acid, (r)/(s)-3-hydroxy-3-methylhexanoic acid ((r)/(s)-hmha), and the human specific volatile thiol, (r)/(s)-3-methyl-3-sulfanylhexan-1-ol ((r)/(s)-msh), were recently identified as major components of human sweat malodor. their 2 corresponding precursors were subsequently isolated from sterile and odorless axillary secretions. the purpose of this work was to analyze these 2 odor precursors in 49 male and female volunteers over a period of 3 years to elucidate to which extent ... | 2009 | 19147808 |
| recombinant glutamine synthetase (gs) from c. glutamicum existed as both hexamers & dedocamers and c-terminal his-tag enhanced inclusion bodies formation in e. coli. | in order to investigate the effect of his-tag on glutamine synthetase (gs, ec 6.3.1.2) from corynebacterium glutamicum, recombinant escherichia coli strains overexpressing gsim, hgsim (gs fused with n-terminal his-tag), gsimh (gs fused with c-terminal his-tag), and hgsimh (gs fused with n-terminal & c-terminal his-tags) were constructed, respectively. under similar expression conditions, gsim and hgsim were partially solubly expressed; no soluble gsimh and hgsimh were observed, based on the resu ... | 2009 | 19148777 |
| complex expression control of the corynebacterium glutamicum aconitase gene: identification of rama as a third transcriptional regulator besides acnr and ripa. | expression of the aconitase gene acn of corynebacterium glutamicum was previously shown to be repressed by the tetr-type regulator acnr in response to a yet unknown stimulus and by the arac-type regulator ripa in response to iron limitation. here we have identified a third transcriptional regulator of aconitase, rama. the rama protein was enriched by dna affinity chromatography with the acn promoter region from protein extracts of acetate-grown cells but not or only weakly from extracts of gluco ... | 2009 | 19095019 |
| first trial in the developmental phase of the "performance evaluation program" based on the iclas animal quality network program: self-assessment of microbiological monitoring methods using test samples supplied by iclas. | the first trial in the developmental phase of the "performance evaluation program" based on the new programs of the international council for laboratory animal science (iclas) was carried out. iclas supplied test samples to each diagnostic laboratory for self-assessment of microbiological monitoring methods. we found that 1 mouse serum sample was positive for mouse minute virus and another was positive for mycoplasma pulmonis antibodies, and 1 rat serum sample was positive for sendai virus antib ... | 2009 | 19151511 |
| in vitro activity of tigecycline and 10 other antimicrobials against clinical isolates of the genus corynebacterium. | we studied the in vitro activity of tigecycline and 10 other commonly used antibiotics against 135 clinical isolates of non-diphtheria corynebacterium spp. using the etest system. tigecycline minimum inhibitory concentrations for 50% and 90% of the organisms (mic(50) and mic(90) values, respectively, in mg/l) were: corynebacterium urealyticum, 0.094 and 0.125; corynebacterium amycolatum, 0.125 and 2; corynebacterium jeikeium, 0.094 and 0.75; corynebacterium coyleae, 0.064 and 0.064; corynebacter ... | 2009 | 19153032 |
| crystallization and preliminary crystallographic analysis of cghle, a homoserine acetyltransferase homologue, from corynebacterium glutamicum. | cghle is an enzyme that is encoded by gene cg0961 from corynebacterium glutamicum. the physiological function of cghle is so far unclear. bioinformatic annotations based on sequence homology indicated that cghle may be an acetyl-coa:homoserine acetyl transferase and as such may be involved in methionine biosynthesis, but recent evidence has shown that it is an esterase that catalyzes the hydrolysis of acetyl esters. here, the crystallization of cghle in two orthorhombic crystal forms, a trigonal ... | 2009 | 19153452 |
| diffusion-weighted mri in the diagnosis of cholesteatomas. | the diagnosis of a cholesteatoma can be difficult in cases with an intact tympanic membrane. the aim of our study was to examine whether diffusion-weighted mri can confirm the diagnosis of a cholesteatoma. | 2009 | 19158448 |
| pathway identification combining metabolic flux and functional genomics analyses: acetate and propionate activation by corynebacterium glutamicum. | corynebacterium glutamicum can utilize acetic acid and propionic acid for growth and amino acid production. growth on acetate as sole carbon source requires acetate activation by acetate kinase (ak) and phosphotransacetylase (pta), encoded in the pta-ack operon. genetic and enzymatic studies showed that these enzymes also catalyze propionate activation and were required for growth on propionate as sole carbon source. however, when glucose was present as a co-substrate strain lacking the ak-pta p ... | 2009 | 19162097 |
| in vitro activity of ciprofloxacin, moxifloxacin, vancomycin and erythromycin against planktonic and biofilm forms of corynebacterium urealyticum. | to study the ability of corynebacterium urealyticum to produce biofilms and to compare the in vitro activity of antimicrobials against planktonic and biofilm-associated organisms. | 2009 | 19056748 |
| l-valine production during growth of pyruvate dehydrogenase complex-deficient corynebacterium glutamicum in the presence of ethanol or by inactivation of the transcriptional regulator sugr. | pyruvate dehydrogenase complex-deficient strains of corynebacterium glutamicum produce l-valine from glucose only after depletion of the acetate required for growth. here we show that inactivation of the deor-type transcriptional regulator sugr or replacement of acetate by ethanol already in course of the growth phase results in efficient l-valine production. | 2009 | 19088318 |
| isolation of corynebacterium ureicelerivorans from normally sterile sites in humans. | fifteen corynebacterium ureicelerivorans isolates were recovered in pure culture from six patients during a five-year period. five patients had bacteremia and the other was an infection of ascitic fluid. the api coryne numerical profile obtained corresponds to the profile for c. bovis, while biolog gp2 identified four out of the six isolates as c. jeikeium. the organisms were molecular identified by 16s rdna and rpob. the present report also includes information on new phenotypic tests and, for ... | 2009 | 19089476 |
| novel macroarray-based method of corynebacterium diphtheriae genotyping: evaluation in a field study in belarus. | in this study, we evaluated a novel macroarray-based spoligotyping method for corynebacterium diphtheriae strain typing. a total of 20 c. diphtheriae biotype gravis toxigenic isolates collected in belarus from suspected foci of diphtheria infection (diphtheria cases, carriers, or contacts) were subjected to dna fingerprinting. all strains had an identical ribotyping profile that was identified as ribotype 'rossija' by comparison with the international ribotype database at the institut pasteur of ... | 2009 | 19089478 |
| a simple mycobacterial monomycolated glycerol lipid has potent immunostimulatory activity. | it is a long held belief that the strong immunostimulatory activity of the mycobacterium bovis bacillus calmette-guérin vaccine and freund's complete adjuvant is due to specific mycobacterial cell envelope components, such as lipids and polysaccharides. implicated mycobacterial lipids include, among others, the so-called cord factor or trehalose dimycolate, but limited information is available regarding the precise molecular nature of the stimulatory components responsible for the interaction wi ... | 2009 | 19109174 |
| clonal multidrug-resistant corynebacterium striatum strains, italy. | we assessed the clinical relevance and performed molecular characterization of 36 multidrug-resistant strains of corynebacterium striatum. pulsed-field gel electrophoresis confirmed a single clone, possessing erm(x), teta/b, cmxa/b, and apha1 genes, but few related subclones. this strain is emerging as a pathogen in italy. | 2009 | 19116057 |
| elastase digests: new ammunition for shotgun membrane proteomics. | despite many advances in membrane proteomics during the last decade the fundamental problem of accessing the transmembrane regions itself has only been addressed to some extent. the present study establishes a method for the nano-lc-based analysis of complex membrane proteomes on the basis of a methanolic porcine pancreatic elastase digest to increase transmembrane coverage. halobacterium salinarium purple and corynebacterium glutamicum membranes were successfully analyzed by using the new proto ... | 2009 | 19116210 |
| characterization of the laci-type transcriptional repressor rbsr controlling ribose transport in corynebacterium glutamicum atcc 13032. | the gene products of the rbsracbd (rbs) operon of c. glutamicum (cg1410-cg1414) encode a ribose-specific atp-binding cassette (abc) transport system and its corresponding regulatory protein (rbsr). deletion of the structural genes rbsacbd prohibited ribose uptake. deletion of the regulatory gene rbsr resulted in an increased mrna level of the whole operon. analysis of the promoter region of the rbs operon by electrophoretic mobility shift assays identified a catabolite-responsive element (cre)-l ... | 2009 | 19118356 |
| metabolic engineering of the l-valine biosynthesis pathway in corynebacterium glutamicum using promoter activity modulation. | the previously constructed strain corynebacterium glutamicumilvnm13 with acetohydroxy acid synthase, resistant to inhibition by all three branched-chain amino acids (l-valine, l-isoleucine and l-leucine), was used as a basis to develop a new type of valine producer by genetic engineering. the main strategy was to modulate expression of the genes involved in the biosynthesis of branched-chain amino acids. the activity of the promoters p-ilvd (dihydroxyacid dehydratase) and p-ilve (transaminase) w ... | 2009 | 19121344 |
| the serine hydroxymethyltransferase gene glya in corynebacterium glutamicum is controlled by glyr. | serine hydroxymethyltransferase (shmt) occupies a central position in one-carbon metabolism, and we here study its regulation in corynebacterium glutamicum. enzyme quantifications revealed an about 3-fold increase of shmt activity during exponential growth with a further increase at the onset of the stationary phase. the shmt encoding glya gene was shown to be transcribed as a monocistronic mrna, and its transcriptional start site was determined. using dna affinity chromatography the regulator g ... | 2009 | 19124047 |
| corynebacterium ulceribovis sp. nov., isolated from the skin of the udder of a cow with a profound ulceration. | a coryneform bacterium isolated from a cow with a profound ulceration was characterized by using phenotypic and molecular taxonomic methods. chemotaxonomic investigations revealed the presence of cell-wall chemotype iv and short-chain mycolic acids consistent with the genus corynebacterium. comparative 16s rrna gene sequencing showed that the organism formed a hitherto unknown subline within the genus corynebacterium. sequence divergence values of greater than 3.6 % from recognized corynebacteri ... | 2009 | 19126719 |
| the puzzle of ligand binding to corynebacterium ammoniagenes fad synthetase. | in bacteria, riboflavin phosphorylation and subsequent conversion of fmn into fad are carried out by fad synthetase, a single bifunctional enzyme. both reactions require atp and mg(2+). the n-terminal domain of fad synthetase appears to be responsible for the adenylyltransferase activity, whereas the c-terminal domain would be in charge of the kinase activity. binding to corynebacterium ammoniagenes fad synthetase of its products and substrates, as well as of several analogues, is analyzed. bind ... | 2009 | 19136717 |
| reengineering of a corynebacterium glutamicum l-arginine and l-citrulline producer. | toward the creation of a robust and efficient producer of l-arginine and l-citrulline (arginine/citrulline), we have performed reengineering of a corynebacterium glutamicum strain by using genetic information of three classical producers. sequence analysis of their arg operons identified three point mutations (argr123, argg92(up), and argg45) in one producer and one point mutation (argb26 or argb31) in each of the other two producers. reconstitution of the former three mutations or of each argb ... | 2009 | 19139237 |
| direct production of cadaverine from soluble starch using corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase. | here, we demonstrated the one-step production of cadaverine from starch using a corynebacterium glutamicum strain coexpressing streptococcus bovis 148 alpha-amylase (amya) and escherichia coli k-12 lysine decarboxylase (cada). we constructed the e. coli-c. glutamicum shuttle vector, which produces cada under the control of the high constitutive expression (hce) promoter, and transformed this vector into c. glutamicum css secreting amya. the engineered c. glutamicum expressed both cada and amya, ... | 2009 | 18989633 |
| multiple abscesses caused by salmonella enterica and corynebacterium pseudotuberculosis in a dromedary camel. | a rare case of arthritis, peri-arthiritis and pleurits associated with salmonella enterica and corynebacterium pseudotuberculosis infection in a dromedary camel is reported. articular infections caused by non-typhoidal salmonella have been exceptionally described in human medicine. to our knowledge, this would be the first description of articular infections associated with non-thyphoidal salmonella in other mammals than humans. possible pathogenesis of the infection is discussed. | 2009 | 18989744 |
| in silico genome-scale reconstruction and validation of the corynebacterium glutamicum metabolic network. | a genome-scale metabolic model of the gram-positive bacteria corynebacterium glutamicum atcc 13032 was constructed comprising 446 reactions and 411 metabolites, based on the annotated genome and available biochemical information. the network was analyzed using constraint based methods. the model was extensively validated against published flux data, and flux distribution values were found to correlate well between simulations and experiments. the split pathway of the lysine synthesis pathway of ... | 2009 | 18985611 |
| crystal structure of the caseinolytic protease gene regulator, a transcriptional activator in actinomycetes. | human pathogens of the genera corynebacterium and mycobacterium possess the transcriptional activator clgr (clp gene regulator) which in corynebacterium glutamicum has been shown to regulate the expression of the clpcp protease genes. clgr specifically binds to pseudo-palindromic operator regions upstream of clpc and clpp1p2. here, we present the first crystal structure of a clgr protein from c. glutamicum. the structure was determined from two different crystal forms to resolutions of 1.75 and ... | 2009 | 19019826 |
| effectiveness of the antibiotic lock therapy for the treatment of port-related enterococci, gram-negative, or gram-positive bacilli bloodstream infections. | the purpose of this study is to evaluate the efficacy of antibiotic lock therapy to treat port-related enterococci, gram-negative, or gram-positive bacilli bloodstream infections. | 2009 | 19026506 |
| in vivo generation of flavoproteins with modified cofactors. | to understand flavoprotein mechanisms and reactivity, biochemical and biophysical methods are usually employed, and differences between wild-type and mutated proteins with altered primary structures are placed under specific consideration. alternatively, the cofactor can be modified, and modified flavoproteins can be studied accordingly. here we present an efficient and general method for modifying the cofactor of flavoproteins in vivo. the modified cofactor is incorporated into apoprotein durin ... | 2009 | 19027027 |
| identification and characterization of a bacterial transport system for the uptake of pyruvate, propionate, and acetate in corynebacterium glutamicum. | the metabolism of monocarboxylic acids is of central importance for bacteria in their natural habitat as well as during biotechnological production. although biosynthesis and degradation are well understood, the transport of such compounds is still a matter of discussion. here we present the identification and characterization of a new transport system in corynebacterium glutamicum with high affinity for acetate and propionate and with lower affinity for pyruvate. biochemical analysis of this mo ... | 2009 | 19028892 |
| characterization of crustins from the hemocytes of the spider crab, hyas araneus, and the red king crab, paralithodes camtschaticus. | crustins are distributed across the decapods and are believed to play a significant part in the humoral defense system of their host. in this study, two crustin isoforms from hyas araneus hemocytes were purified and tested for antimicrobial activity against selected microorganisms. they show both antibacterial and antifungal activity, with highest activity against the gram-positive bacteria corynebacterium glutamicum. sequencing of the transcripts showed them to have a mature peptide of 90 amino ... | 2009 | 19041340 |
| a combination of metabolome and transcriptome analyses reveals new targets of the corynebacterium glutamicum nitrogen regulator amtr. | the effects of a deletion of the amtr gene, encoding the master regulator of nitrogen control in corynebacterium glutamicum, were investigated by metabolome and transcriptome analyses. compared to the wild type, different metabolite patterns were observed in respect to glycolysis, pentose phosphate pathway, citric acid cycle, and most amino acid pools. not all of these alterations could be attributed to changes at the level of mrna and must be caused by posttranscriptional regulatory processes. ... | 2009 | 19041910 |
| dual transcriptional control of the acetaldehyde dehydrogenase gene ald of corynebacterium glutamicum by rama and ramb. | corynebacterium glutamicum has been shown to grow with ethanol as the sole or as additional carbon and energy source and accordingly, to possess both alcohol dehydrogenase and acetaldehyde dehydrogenase (aldh) activities, which are responsible for the two-step ethanol oxidation to acetate. here we identify and functionally analyze the c. glutamicum aldh gene (cg3096, ald), its expression and its regulation. directed inactivation of the chromosomal ald gene led to the absence of detectable aldh a ... | 2009 | 19041911 |
| corynebacterium diphtheriae: genome diversity, population structure and genotyping perspectives. | the epidemic re-emergence of diphtheria in russia and the newly independent states (nis) of the former soviet union in the 1990s demonstrated the continued threat of this thought to be rare disease. the bacteriophage encoded toxin is a main virulence factor of corynebacterium diphtheriae, however, an analysis of the first complete genome sequence of c. diphtheriae revealed a recent acquisition of other pathogenicity factors including iron-uptake systems, adhesins and fimbrial proteins as indeed ... | 2009 | 19007916 |
| [pseudarthrosis after fracture of the distal radius: a case report]. | we report on a seventy-year-old woman who developed a non-union of the distal radius with infection of the ulnocarpal joint after having sustained a fracture. the fistula, the distal ulna and the ulnocarpal joint were resected. two months later we removed the non-union and carried out open reduction and internal fixation of the radius with a palmar plate. good functional and cosmetic results were achieved. | 2009 | 19012229 |
| [axillary lymphadenitis due to corynebacterium pseudotuberculosis in a 63-year-old patient]. | a 63-year-old woman living in a rural area presented in 2004 with a granulomatous necrotizing axillary lymphadenitis caused by corynebacterium pseudotuberculosis, a gram positive bacillus belonging to the group of corynebacterium diphtheriae, which is found primarily in animal infections. in the human being, around 30 cases of infection due to c. pseudotuberculosis have been described, mainly among people working in contact with infected animals. for this reason, this infection, which induces ex ... | 2009 | 19013041 |
| the whca gene plays a negative role in oxidative stress response of corynebacterium glutamicum. | in this study, we analyzed the whca gene from corynebacterium glutamicum, which codes for a homologue of the whib-family of proteins. deletion of the gene did not affect the growth of the mutant cells, indicating that the whca gene was not essential under ordinary growth conditions. however, cells overexpressing the protein not only showed retarded growth as compared with the wild-type or the deltawhca mutant cells but also showed increased sensitivity to a variety of oxidants, such as diamide, ... | 2009 | 19016879 |
| relapsing peritonitis with corynebacterium aquaticum in a boy receiving automated peritoneal dialysis. | 2009 | 19048297 | |
| metabolic quenching of corynebacterium glutamicum: efficiency of methods and impact of cold shock. | representative and valid cytoplasmic concentrations are essential for ensuring the significance of results in the field of metabolome analysis. one of the most crucial points in this respect is the sampling itself. a rapid and sudden stopping of the metabolism on a timescale that is much faster than the conversion rates of investigated metabolites is worthwhile. this can be achieved by applying of cold methanol quenching combined with reproducible, fast, and automated sampling. unfortunately, qu ... | 2009 | 19050933 |
| myo-inositol facilitators iolt1 and iolt2 enhance d-mannitol formation from d-fructose in corynebacterium glutamicum. | reduction of d-fructose to d-mannitol by whole-cell biotransformation with recombinant resting cells of corynebacterium glutamicum atcc13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. however, d-mannitol formation is limited by the uptake of d-fructose in its unphosphorylated form, because additional expression of the sugar facilitator from zymomonas mobilis resulted in a significantly increased productivity. here we identified simil ... | 2009 | 19054080 |
| involvement of the luxr-type transcriptional regulator rama in regulation of expression of the gapa gene, encoding glyceraldehyde-3-phosphate dehydrogenase of corynebacterium glutamicum. | sugr, rama, glxr, gntr1, and a marr-type transcriptional regulator bind to the promoter region of the gapa gene encoding glyceraldehyde-3-phosphate dehydrogenase (gapdh), essential for glycolysis in corynebacterium glutamicum. we previously showed that sugr, a transcriptional repressor of phosphotransferase system genes for the sugar transport system, is involved in the downregulation of gapa expression in the absence of sugar. in this study, the role of rama in the expression of the gapa gene w ... | 2009 | 19047347 |
| acetohydroxyacid synthase, a novel target for improvement of l-lysine production by corynebacterium glutamicum. | the influence of acetohydroxy acid synthase (ahas) on l-lysine production by corynebacterium glutamicum was investigated. an ahas with a deleted c-terminal domain in the regulatory subunit ilvn was engineered by truncating the ilvn gene. compared to the wild-type ahas, the newly constructed enzyme showed altered kinetic properties, i.e., (i) an about twofold-lower k(m) for the substrate pyruvate and an about fourfold-lower v(max); (ii) a slightly increased k(m) for the substrate alpha-ketobutyra ... | 2009 | 19047397 |
| maturing dynamics of surface microflora in fontina pdo cheese studied by culture-dependent and -independent methods. | to study the evolution of rind microbial communities in fontina pdo cheese. | 2009 | 19054234 |
| effect of ph on the binding mechanisms in biosorption of reactive orange 16 by corynebacterium glutamicum. | binding mechanisms of reactive orange 16 (ro 16) by the protonated waste biomass of corynebacterium glutamicum were investigated. the solution ph was found to strongly influence the uptake of ro 16 by c. glutamicum. the biosorption of the dye was reversible at ph <7 but irreversible under basic ph conditions. at acidic ph, the electrostatic interaction was found to be a major binding mechanism. the maximum sorption capacities of the biomass were evaluated to be 156.6+/-6.2 and 64.0+/-2.4 mg/g at ... | 2009 | 19062035 |
| a 2d reversed-phase x ion-pair reversed-phase hplc-maldi tof/tof-ms approach for shotgun proteome analysis. | the separation of complex peptide mixtures in shotgun proteome analysis using a 2d separation scheme encompassing reversed-phase x ion-pair reversed-phase (ip-rp) liquid chromatography coupled online to electrospray ion trap mass spectrometry (ms) has been shown earlier to be superior in terms of separation efficiency and technical robustness compared to the classically used separation scheme encompassing strong cation exchange x ip-rp-chromatography in shotgun proteome analysis. in the present ... | 2009 | 19066860 |
| identification of new secreted proteins and secretion of heterologous amylase by c. glutamicum. | in this study, secreted corynebacterium glutamicum proteins were investigated by two-dimensional gel electrophoresis. around 100 spots observed in the ph range 4.5-5.5 had molecular masses that varied from 10 to 50 kda. upon n-terminal amino acid sequence analysis by edman degradation, two of them were hits to two hypothetical proteins encoded by cgr_1176 and cgr_2070 on c. glutamicum r genome, respectively. active-form alpha-amylase derived from geobacillus stearothermophilus was successfully s ... | 2009 | 19066885 |
| regulation and activity of a zinc uptake regulator, zur, in corynebacterium diphtheriae. | regulation of metal ion homeostasis is essential to bacterial cell survival, and in most species it is controlled by metal-dependent transcriptional regulators. in this study, we describe a corynebacterium diphtheriae ferric uptake regulator-family protein, zur, that controls expression of genes involved in zinc uptake. by measuring promoter activities and mrna levels, we demonstrate that zur represses transcription of three genes (zrg, cmra, and troa) in zinc-replete conditions. all three of th ... | 2009 | 19074382 |
| towards the integrated analysis, visualization and reconstruction of microbial gene regulatory networks. | to handle changing environmental surroundings and to manage unfavorable conditions, microbial organisms have evolved complex transcriptional regulatory networks. to comprehensively analyze these gene regulatory networks, several online available databases and analysis platforms have been implemented and established. in this article, we address the typical cycle of scientific knowledge exploration and integration in the area of procaryotic transcriptional gene regulation. we briefly review five p ... | 2009 | 19074493 |
| tatabc overexpression improves corynebacterium glutamicum tat-dependent protein secretion. | the twin-arginine translocation (tat) pathway in corynebacterium glutamicum has been described previously. the minimal functional tat system in c. glutamicum required tata and tatc but did not require tatb, although this component was required for maximal efficiency of tat-dependent secretion. we previously demonstrated that chryseobacterium proteolyticum pro-protein glutaminase (pro-pg) and streptomyces mobaraensis pro-transglutaminase (pro-tg) could be secreted via the tat pathway in c. glutam ... | 2009 | 19074606 |
| antigens of corynebacterium pseudotuberculosis and prospects for vaccine development. | corynebacterium pseudotuberculosis continues to cause considerable economic losses in ovine and caprine herds worldwide, causing caseous lymphadenitis. nevertheless, the immunology of this disease is relatively unknown. novel antigens may provide vaccines that are more effective and improve diagnostic methods for better control of this disease. the available commercial vaccines are not able to fully protect susceptible animals, cannot be used in all host species and are not licensed for use in m ... | 2009 | 19196200 |
| htaa is an iron-regulated hemin binding protein involved in the utilization of heme iron in corynebacterium diphtheriae. | many human pathogens, including corynebacterium diphtheriae, the causative agent of diphtheria, use host compounds such as heme and hemoglobin as essential iron sources. in this study, we examined the corynebacterium hmu hemin transport region, a genetic cluster that contains the hmutuv genes encoding a previously described abc-type hemin transporter and three additional genes, which we have designated htaa, htab, and htac. the hmu gene cluster is composed of three distinct transcriptional units ... | 2009 | 19201805 |
| roles of maltodextrin and glycogen phosphorylases in maltose utilization and glycogen metabolism in corynebacterium glutamicum. | corynebacterium glutamicum transiently accumulates large amounts of glycogen, when cultivated on glucose and other sugars as a source of carbon and energy. apart from the debranching enzyme glgx, which is required for the formation of maltodextrins from glycogen, alpha-glucan phosphorylases were assumed to be involved in glycogen degradation, forming alpha-glucose 1-phosphate from glycogen and from maltodextrins. we show here that c. glutamicum in fact possesses two alpha-glucan phosphorylases, ... | 2009 | 19202084 |
| microarray studies reveal a 'differential response' to moderate or severe heat shock of the hrca- and hspr-dependent systems in corynebacterium glutamicum. | genome-wide transcription profile analysis of the heat-shocked wild-type strain under moderate (40 degrees c) and severe heat stress (50 degrees c) revealed that a large number of genes are differentially expressed after heat shock. of these, 358 genes were upregulated and 420 were downregulated in response to moderate heat shock (40 degrees c) in corynebacterium glutamicum. our results confirmed the hrca/controlling inverted repeat of chaperone expression (circe)-dependent and hspr/hspr-associa ... | 2009 | 19202085 |
| composition and characteristics of urinary calculi from guinea pigs. | to determine the mineral composition of calculi, anatomic locations of the calculi, and findings of urinalysis and bacteriologic culture of urine and calculi in guinea pigs with urolithiasis. | 2009 | 19210239 |
| [corynebacterium urealyticum: from the bedside to complete genome sequence]. | 2009 | 19217996 | |
| the bacteriology of chronic rhinosinusitis with and without nasal polyps. | to compare the bacteriologic findings in ethmoidal biopsy specimens and nasal lavage samples from healthy control patients and from patients with chronic rhinosinusitis (crs) with nasal polyps (crsnp+) and without nasal polyps (crsnp-). | 2009 | 19221239 |
| molecular mechanism of sugr-mediated sugar-dependent expression of the ldha gene encoding l-lactate dehydrogenase in corynebacterium glutamicum. | this paper reports on the transcriptional regulation mechanism of the corynebacterium glutamicum ldha gene encoding l: -lactate dehydrogenase responsible for production of l: -lactate. dna affinity purification allowed us to identify sugr, a global repressor of genes involved in sugar uptake and glycolysis, as a protein binding to the ldha promoter region. whereas ldha gene expression and ldha promoter activity were completely repressed during growth of wild-type cells in the absence of sugar, n ... | 2009 | 19221735 |
| corynebacterium pseudodiphtheriticum isolated from relevant clinical sites of infection: a human pathogen overlooked in emerging countries. | to examine the occurrence of and to determine the antimicrobial susceptibility of corynebacterium pseudodiphtheriticum among patients with bacterial infections at a teaching hospital. | 2009 | 19228291 |
| evaluation of hepatic disease in mice following intradermal inoculation with corynebacterium pseudotuberculosis. | to evaluate clinical, microbiologic, and pathologic outcomes in mice after inoculation with 4 equine-origin corynebacterium pseudotuberculosis strains. | 2009 | 19231959 |
| expression and localization of the corynebacterium glutamicum ncgl1221 protein encoding an l-glutamic acid exporter. | corynebacterium glutamicum strains are used for the fermentative production of l-glutamate. biotin limitation and addition of fatty acid ester surfactants can induce l-glutamic acid secretion. however, the mechanism of l-glutamate secretion remains unclear. it was recently reported that the ncgl1221 protein, a mechanosensitive channel homolog, was considered to be functional as an important l-glutamate exporter. however, the structure of the ncgl1221 protein has not been studied recently. in thi ... | 2009 | 19233628 |
| formation and metabolism of methylmalonyl coenzyme a in corynebacterium glutamicum. | genome sequence information suggests that b(12)-dependent mutases are present in a number of bacteria, including members of the suborder corynebacterineae like mycobacterium tuberculosis and corynebacterium glutamicum. we here functionally identify a methylmalonyl coenzyme a (coa) mutase in c. glutamicum that is retained in all of the members of the suborder corynebacterineae and is encoded by ncgl1471, ncgl1472, and ncgl1470. in addition, we observe the presence of methylmalonate in c. glutamic ... | 2009 | 19233926 |
| microbiology of the external ear canal in six african elephants (loxodonta africana). | samples collected from both external ear canals of six adult female african elephants (loxodonta africana) were cultured for fungi, yeasts and aerobic and anaerobic bacteria. all the samples produced heavy growths of several aerobic bacteria, but anaerobic bacteria were rare and no fungi or yeasts were isolated. the most common bacterium isolated was staphylococcus epidermidis, which was cultured from 11 of the 12 ears. acinetobacter calcoaceticus lwoffi, alpha-haemolytic streptococcus and coryn ... | 2009 | 19234325 |
| the oligomeric assembly of the novel haem-degrading protein hbps is essential for interaction with its cognate two-component sensor kinase. | hbps, a novel protein of previously unknown function from streptomyces reticuli, is up-regulated in response to haemin- and peroxide-based oxidative stress and interacts with the sens/senr two-component signal transduction system. in this study, we report the high-resolution crystal structures (2.2 and 1.6 a) of octomeric hbps crystallized in the presence and in the absence of haem and demonstrate that iron binds to surface-exposed lysine residues of an octomeric assembly. based on an analysis o ... | 2009 | 19244623 |
| scanning the corynebacterium glutamicum r genome for high-efficiency secretion signal sequences. | systematic screening of secretion proteins using an approach based on the completely sequenced genome of corynebacterium glutamicum r revealed 405 candidate signal peptides, 108 of which were able to heterologously secrete an active-form alpha-amylase derived from geobacillus stearothermophilus. these comprised 90 general secretory (sec)-type, 10 twin-arginine translocator (tat)-type and eight sec-type with presumptive lipobox peptides. only sec- and tat-type signals directed high-efficiency sec ... | 2009 | 19246745 |
| pilot study of association of bacteria on breast implants with capsular contracture. | capsular contracture is the most common and frustrating complication in women who have undergone breast implantation. its cause and, accordingly, treatment and prevention remain to be elucidated fully. the aim of this prospective observational pilot study was to test the hypothesis that the presence of bacteria on breast implants is associated with capsular contracture. we prospectively studied consecutive patients who underwent breast implant removal for reasons other than overt infection at th ... | 2009 | 19261794 |
| molecular basis of transport and regulation in the na(+)/betaine symporter betp. | osmoregulated transporters sense intracellular osmotic pressure and respond to hyperosmotic stress by accumulation of osmolytes to restore normal hydration levels. here we report the determination of the x-ray structure of a member of the family of betaine/choline/carnitine transporters, the na(+)-coupled symporter betp from corynebacterium glutamicum, which is a highly effective osmoregulated uptake system for glycine betaine. glycine betaine is bound in a tryptophan box occluded from both side ... | 2009 | 19262666 |
| images in cardio-thoracic surgery: novacor left ventricular assist device inflow valve endocarditis. | 2009 | 19269841 | |
| regulation of corynebacterium glutamicum heat shock response by the extracytoplasmic-function sigma factor sigh and transcriptional regulators hspr and hrca. | heat shock response in corynebacterium glutamicum was characterized by whole-genome expression analysis using a dna microarray. it was indicated that heat shock response of c. glutamicum included not only upregulation of heat shock protein (hsp) genes encoding molecular chaperones and atp-dependent proteases, but it also increased and decreased expression of more than 300 genes related to disparate physiological functions. an extracytoplasmic-function sigma factor, sigh, was upregulated by heat ... | 2009 | 19270092 |
| potassium transport in corynebacterium glutamicum is facilitated by the putative channel protein cglk, which is essential for ph homeostasis and growth at acidic ph. | we studied the requirement for potassium and for potassium transport activity for the biotechnologically important bacterium corynebacterium glutamicum, which is used for large-scale production of amino acids. different from many other bacteria, at alkaline or neutral ph, c. glutamicum is able to grow without the addition of potassium, resulting in very low cytoplasmic potassium concentrations. in contrast, at acidic ph, the ability for growth was found to depend on the presence of k+. for the f ... | 2009 | 19270097 |
| arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange. | we identified the first enzymes that use mycothiol and mycoredoxin in a thiol/disulfide redox cascade. the enzymes are two arsenate reductases from corynebacterium glutamicum (cg_arsc1 and cg_arsc2), which play a key role in the defense against arsenate. in vivo knockouts showed that the genes for cg_arsc1 and cg_arsc2 and those of the enzymes of the mycothiol biosynthesis pathway confer arsenate resistance. with steady-state kinetics, arsenite analysis, and theoretical reactivity analysis, we u ... | 2009 | 19286650 |
| de novo biosynthesis of vanillin in fission yeast (schizosaccharomyces pombe) and baker's yeast (saccharomyces cerevisiae). | vanillin is one of the world's most important flavor compounds, with a global market of 180 million dollars. natural vanillin is derived from the cured seed pods of the vanilla orchid (vanilla planifolia), but most of the world's vanillin is synthesized from petrochemicals or wood pulp lignins. we have established a true de novo biosynthetic pathway for vanillin production from glucose in schizosaccharomyces pombe, also known as fission yeast or african beer yeast, as well as in baker's yeast, s ... | 2009 | 19286778 |
| corynebacterium bovis shoulder prosthetic joint infection: the first reported case. | we report the first case of corynebacterium bovis shoulder prosthetic joint infection. the organism was isolated from intraoperative tissue culture and from the removed prosthesis using sonication. a 2-stage exchange and 3 months of antibiotic therapy were performed. c. bovis may cause implant-associated infections, which can manifest as low-grade infection. | 2009 | 19304438 |
| exopolyphosphatases ppx1 and ppx2 from corynebacterium glutamicum. | corynebacterium glutamicum accumulates up to 300 mm of inorganic polyphosphate (polyp) in the cytosol or in granules. the gene products of cg0488 (ppx1) and cg1115 (ppx2) were shown to be active as exopolyphosphatases (ppx), as overexpression of either gene resulted in higher exopolyphosphatase activities in crude extracts and deletion of either gene with lower activities than those of the wild-type strain. ppx1 and ppx2 from c. glutamicum share only 25% identical amino acids and belong to diffe ... | 2009 | 19304823 |
| toxigenic corynebacterium ulcerans isolated from the domestic dog for the first time in japan. | 2009 | 19305066 | |
| expression of a multi-epitope dpt fusion protein in transplastomic tobacco plants retains both antigenicity and immunogenicity of all three components of the functional oligomer. | expression of genes in plant chloroplasts provides an opportunity for enhanced production of target proteins. we report the introduction and expression of a fusion dpt protein containing immunoprotective exotoxin epitopes of corynebacterium diphtheriae, bordetella pertussis, and clostridium tetani in tobacco chloroplasts. using biolistic-mediated transformation, a plant-optimized synthetic dpt gene was successfully transferred to tobacco plastomes. putative transplastomic t0 plants were identifi ... | 2009 | 19306020 |
| structure of thermotoga maritima tm0439: implications for the mechanism of bacterial gntr transcription regulators with zn2+-binding fcd domains. | the gntr superfamily of dimeric transcription factors, with more than 6200 members encoded in bacterial genomes, are characterized by n-terminal winged-helix dna-binding domains and diverse c-terminal regulatory domains which provide a basis for the classification of the constituent families. the largest of these families, fadr, contains nearly 3000 proteins with all-alpha-helical regulatory domains classified into two related pfam families: fadr_c and fcd. only two crystal structures of fadr-fa ... | 2009 | 19307717 |
| genetic and phenotypic diversity of fenitrothion-degrading bacteria isolated from soils. | twenty-seven fenitrothion-degrading bacteria were isolated from different soils, and their genetic and phenotypic characteristics were investigated. analysis of the 16s rdna sequence showed that the isolates were related to members of the genera burkholderia, pseudomonas, sphingomonas, cupriavidus, corynebacterium, and arthrobacter. among the 27 isolates, 12 different chromosomal dna fingerprinting patterns were obtained by polymerase chain reaction (pcr) amplification of repetitive extragenic p ... | 2009 | 19307758 |
| osmosensing and osmosignaling in corynebacterium glutamicum. | the gram-positive soil bacterium corynebacterium glutamicum is used in microbial biotechnology for the large-scale production of amino acids, e.g., l: -glutamate and l: -lysine. we have studied the response of this organism to hyperosmotic challenge at the level of both transcription and protein activity. two systems responding to hyperosmotic stress in c. glutamicum are reviewed here, the two component system mtrab and the glycine-betaine uptake system betp. the osmosensory two-component system ... | 2009 | 19308662 |
| the two-phase partitioning system--a powerful technique to purify integral membrane proteins of corynebacterium glutamicum for quantitative shotgun analysis. | we established a single consecutive strategy which assigned the most comprehensive number of integral membrane proteins from gram-positive bacteria to date. for this purpose, we adapted a biphasic partitioning system for the biotechnologically intensively used corynebacterium glutamicum and proved for the first time that such a system is well suited for quantitative comparison. 297 integral membrane proteins were identified by our integrated approach, which depletes stringently cytosolic protein ... | 2009 | 19322788 |
| dual production of poly(3-hydroxybutyrate) and glutamate using variable biotin concentrations in corynebacterium glutamicum. | we previously synthesized poly(3-hydroxybutyrate) [p(3hb)] in recombinant corynebacterium glutamicum, a prominent producer of amino acids. in this study, a two-step cultivation was established for the dual production of glutamate and p(3hb) due to the differences in the optimal concentration of biotin. glutamate was extracellularly produced first under the biotin-limited condition of 0.3 microg/l. production was then shifted to p(3hb) by addition of biotin to a total concentration of 9 microg/l. ... | 2009 | 19332300 |
| regulation of ldh expression during biotin-limited growth of corynebacterium glutamicum. | corynebacterium glutamicum is a biotin-auxotrophic bacterium and some strains efficiently produce glutamic acid under biotin-limiting conditions. in an effort to understand c. glutamicum metabolism under biotin limitation, growth of the type strain atcc 13032 was investigated in batch cultures and a time-course analysis was performed. a transient excretion of organic acids was observed and we focused our attention on lactate synthesis. lactate synthesis was due to the ldh-encoded l-lactate dehyd ... | 2009 | 19332837 |
| [polymorphism of tox and dtxr genes in circulating strains of corynebacterium diphtheriae]. | polymorphism of tox and dtxr genes responsible for diphtheria toxin synthesis was revealed. seven point mutations in tox gene were detected; study of their combinations allowed to determine 10 allelic variants of the tox gene in c. diphtheriae strains. majority of mutations did not lead to changes in substitutions in amino acid sequence of diphtheria toxin. in tox gene from 2 strains of mitis biovar, ribotype "otchakov" isolated in saint-petersburg, mutation in position 1252 (g --> c), which cor ... | 2009 | 19340963 |
| identification and functional analysis of the gene cluster for l-arabinose utilization in corynebacterium glutamicum. | corynebacterium glutamicum atcc 31831 grew on l-arabinose as the sole carbon source at a specific growth rate that was twice that on d-glucose. the gene cluster responsible for l-arabinose utilization comprised a six-cistron transcriptional unit with a total length of 7.8 kb. three l-arabinose-catabolizing genes, araa (encoding l-arabinose isomerase), arab (l-ribulokinase), and arad (l-ribulose-5-phosphate 4-epimerase), comprised the arabda operon, upstream of which three other genes, arar (laci ... | 2009 | 19346355 |
| repeatability of peptide identifications in shotgun proteome analysis employing off-line two-dimensional chromatographic separations and ion-trap ms. | the repeatability of peptide identifications in shotgun proteome analyses employing strong cation-exchange-xion-pair rp hplc hyphenated to esi ms/ms was compared to an alternative scheme, comprising high-ph rp chromatography combined with low-ph ion-pair rp chromatography. equivalent results were obtained with both methods in proteome analysis of corynebacterium glutamicum. from a total number of 1350 to 1850 peptides identified in triplicate analyses of five consecutive fractions chosen from th ... | 2009 | 19360783 |
| dynamic and structural characterization of a bacterial fha protein reveals a new autoinhibition mechanism. | the odhi protein is key regulator of the tca cycle in corynebacterium glutamicum. this highly conserved protein is found in gc rich gram-positive bacteria (e.g., the pathogenic mycobacterium tuberculosis). the unphosphorylated form of odhi inhibits the odha protein, a key enzyme of the tca cycle, whereas the phosphorylated form is inactive. odhi is predicted to be mainly a single fha domain, a module that mediates protein-protein interaction through binding of phosphothreonine peptides, with a d ... | 2009 | 19368890 |
| genetic makeup of the corynebacterium glutamicum lexa regulon deduced from comparative transcriptomics and in vitro dna band shift assays. | the lexa gene of corynebacterium glutamicum atcc 13032 was deleted to create the mutant strain c. glutamicum nj2114, which has an elongated cell morphology and an increased doubling time. to characterize the sos regulon in c. glutamicum, the transcriptomes of nj2114 and a dna-damage-induced wild-type strain were compared with that of a wild-type control using dna microarray hybridization. the expression data were combined with bioinformatic pattern searches for lexa binding sites, leading to the ... | 2009 | 19372162 |
| conjunctival flora in patients with parkinson's disease. | to identify the conjunctival flora in patients with parkinson's disease (pd) and compare it with the conjunctival flora of healthy subjects. | 2009 | 19373572 |
| citrate utilization by corynebacterium glutamicum is controlled by the citab two-component system through positive regulation of the citrate transport genes cith and tctcba. | in this work, the molecular basis of aerobic citrate utilization by the gram-positive bacterium corynebacterium glutamicum was studied. genome analysis revealed the presence of two putative citrate transport systems. the permease encoded by cith belongs to the citrate-mg(2+):h(+)/citrate-ca(2+):h(+) symporter family, whereas the permease encoded by the tctcba operon is a member of the tripartite tricarboxylate transporter family. the expression of cith or tctcba in escherichia coli enabled this ... | 2009 | 19376865 |
| regulation of expression of genes involved in quinate and shikimate utilization in corynebacterium glutamicum. | the utilization of the hydroaromatic compounds quinate and shikimate by corynebacterium glutamicum was investigated. c. glutamicum grew well with either quinate or shikimate as the sole carbon source. the disruption of qsud, encoding quinate/shikimate dehydrogenase, completely suppressed growth with either substrate but did not affect growth with glucose, indicating that the enzyme encoded by qsud catalyzes the first step of the catabolism of quinate/shikimate but is not involved in the shikimat ... | 2009 | 19376919 |
| the cmar gene of corynebacterium ammoniagenes performs a novel regulatory role in the metabolism of sulfur-containing amino acids. | a novel regulatory gene, which performs an essential function in sulfur metabolism, has been identified in corynebacterium ammoniagenes and was designated cmar (cysteine and methionine regulator in c. ammoniagenes). the cmar-disrupted strain (deltacmar) lost the ability to grow on minimal medium, and was identified as a methionine and cysteine double auxotroph. the mutant strain proved unable to convert cysteine to methionine (and vice versa), and lost the ability to assimilate and reduce sulfat ... | 2009 | 19383689 |
| matriptase-deficient mice exhibit ichthyotic skin with a selective shift in skin microbiota. | suppressor of tumorigenicity 14 (st14) encodes matriptase, a serine protease, which regulates processing of profilaggrin to filaggin in vivo. here, we report that transgenic mice with 1% of wild-type st14 levels (st14(hypo/-)) display aberrant processing of profilaggrin and model human ichthyotic skin phenotypes. scaling of the skin appears at 1 week of age with underlying epidermal acanthosis and orthohyperkeratosis as well as a cd4+ t-cell dermal infiltrate. upregulation of antimicrobial pepti ... | 2009 | 19387477 |
| corynebacterium striatum infecting a malignant cutaneous lesion: the emergence of an opportunistic pathogen. | we described a case of a 27-year old male patient with skin and soft tissue infection of a neoplastic lesion caused by corynebacterium striatum, an organism which has been rarely described as a human pathogen. identification was confirmed by dna sequencing. successful treatment with penicillin was achieved. the role of the c. striatum as an emerging opportunistic pathogen is discussed. | 2009 | 19390742 |
| corynebacterium striatum--a classic pathogen eluding diagnosis. | we report one case of infective endocarditis and two cases of arthritis due to corynebacterium striatum. after reviewing all previously reported cases, we illustrate the clinical features typical of infection, including nosocomial risk factors and prominent embolic phenomena in the cases of endocarditis. our cases underscore the importance of prompt diagnosis when confronted with positive blood and synovial cultures. this ubiquitous organism and common contaminant is a rare yet deadly pathogen. | 2009 | 19393478 |
| diphtheria: a zoonotic disease in france? | thanks to vaccination, diphtheria has almost disappeared in france. the case definition, used for mandatory notification, was expanded in 2003 to include toxin-producing strains of corynebacterium ulcerans. we describe the epidemiology of diphtheria in france from 1990 to 2008. no cases occurred between 1990 and 2001. since 2002, 19 cases have been reported: 4 cases due to corynebacterium diphtheriae related to exposure in endemic countries, and 15 cases due to other corynebacteria, including 4 ... | 2009 | 19393707 |
| a game with many players: control of gdh transcription in corynebacterium glutamicum. | glutamate dehydrogenase is a central enzyme connecting nitrogen and carbon metabolism via its precursors ammonium and oxoglutarate and its product glutamate. in corynebacterium glutamicum glutamate dehydrogenase is especially important, since it is a key enzyme for the biotechnological production of the flavour enhancer l-glutamate. in this study, the regulation of gdh transcription was investigated. while originally described as a constitutively expressed gene, we could show that gdh transcript ... | 2009 | 19394370 |
| characterization of the corynebacterium glutamicum deltapimb' deltamgta double deletion mutant and the role of mycobacterium tuberculosis orthologues rv2188c and rv0557 in glycolipid biosynthesis. | in this study, utilizing a corynebacterium glutamicum deltapimb' deltamgta double deletion mutant, we unequivocally assign the in vivo functions of rv2188c as an ac(1)pim(1):mannosyltransferase (originally termed pimb'(mt) [mycobacterium tuberculosis pimb']) and rv0557 as a glcagroac(2):mannosyltransferase (originally termed pimb(mt)), which we have reassigned as pimb(mt) and mgta(mt), respectively, in mycobacterium tuberculosis. | 2009 | 19395496 |
| productivity of cyclohexanone oxidation of the recombinant corynebacterium glutamicum expressing chnb of acinetobacter calcoaceticus. | the biocatalytic efficiency of recombinant corynebacterium glutamicum expressing the chnb gene encoding cyclohexanone monooxygenase (chmo) of acinetobacter calcoaceticus ncimb 9871 was investigated. optimization of an expression system and induction conditions enabled the recombinant biocatalyst to produce chmo to a specific activity of ca. 0.5 u mg(-1) protein. tight control of feeding of an energy source (i.e., glucose) and dissolved oxygen tension during fed-batch culture-based biotransformat ... | 2009 | 19397940 |
| gluconate as suitable potential reduction supplier in corynebacterium glutamicum: cloning and expression of gntp and gntk in escherichia coli. | corynebacterium glutamicum is widely used in the industrial production of amino acids. we have found that this bacterium grows exponentially on a mineral medium supplemented with gluconate. gluconate permease and gluconokinase are expressed in an inducible form and, 6-phosphogluconate dehydrogenase, although constitutively expressed, shows a 3-fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions. interestingly, these activities are lower than ... | 2009 | 19399347 |