Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| electron paramagnetic resonance (epr) spectroscopy of the stable-free radical in the native metallo-cofactor of the manganese-ribonucleotide reductase (mn-rnr) of corynebacterium glutamicum. | ribonucleotide reductases (rnr; ec 1.17.4.1) provide the 2'-deoxyribonucleotides for dna replication of proliferating cells by a uniform radical mechanism using diverse metals. the native metallo-cofactor of the corynebacterium glutamicum rnr contains manganese and is sensitive to edta and radical scavengers. hybrid holoenzymes, capable of ribonucleotide reduction, were composed of the small manganese-containing (r2f) and the large catalytic subunit (r1e) from either of the two corynebacterial r ... | 2009 | 19707921 |
| conversion of phenol to glutamate and proline in corynebacterium glutamicum is regulated by transcriptional regulator argr. | this paper reports a novel integrated metabolic pathway of amino acid production through the utilization of phenol in corynebacterium glutamicum. in the presence of 8.5 mm phenol, the level of glutamate and proline production increased up to 1.2- and 14.7-fold, respectively, compared to the control condition. in addition, their productivities increased 1.6- and 20-fold in the culture medium using phenol as the sole carbon source with 72 microm feso(4) as iron supplementation. chromatin immunopre ... | 2010 | 19707750 |
| optical device for parallel online measurement of dissolved oxygen and ph in shake flask cultures. | we describe a new device with parallel optical measurement of dissolved oxygen (do) and ph in up to nine shake flasks applicable in any conventional shaking incubator. measurement ranges are 0-500% of air saturation for oxygen and 5.5-8.5 for ph. it was used to characterize growth profiles of different l-lysine producing strains of corynebacterium glutamicum, of saccharomyces cerevisiae and of escherichia coli. cultures in unbaffled flasks were highly reproducible. oxygen limitation was indicate ... | 2010 | 19701780 |
| a new, simple, rapid test for detection of dnase activity of microorganisms: dnase tube test. | the dnase test is a simple, economical method that has traditionally been used as a supplemental test to identify pathogenic staphylococcus. this test also aids in the differentiation of closely-related genera within the klebsiella-enterobacter-serratia division of enterobacteriaceae and several other pathogens, including screening of c. diphtheriae. currently dnase activity of microorganisms was tested using dnase agar plate methods. these tests have some drawbacks including the necessity of th ... | 2009 | 19700923 |
| visualizing post genomics data-sets on customized pathway maps by prometra-aeration-dependent gene expression and metabolism of corynebacterium glutamicum as an example. | the rapid progress of post-genomic analyses, such as transcriptomics, proteomics, and metabolomics has resulted in the generation of large amounts of quantitative data covering and connecting the complete cascade from genotype to phenotype for individual organisms. various benefits can be achieved when these "omics" data are integrated, such as the identification of unknown gene functions or the elucidation of regulatory networks of whole organisms. in order to be able to obtain deeper insights ... | 2009 | 19698148 |
| reinforcement of carboxyl groups in the surface of corynebacterium glutamicum biomass for effective removal of basic dyes. | the biomass of corynebacterium glutamicum was treated with poly(amic acid) to improve the biosorption of basic blue 3 (bb3) from aqueous solution. the grafting of poly(amic acid) onto the biomass surface increased the density of the carboxyl groups. the uv-spectrum revealed that strong acidic (ph2) and basic conditions (ph11) resulted in the precipitation of bb3. therefore, ph edge experiments were conducted only within the range 3-10; these results indicated that electrostatic attraction betwee ... | 2009 | 19692228 |
| intracranial hemorrhage following thrombolytic use for stroke caused by infective endocarditis. | background and purpose: stroke is one of the most common neurological manifestations of infective endocarditis. the use of intravenous tissue plasminogen activator (t-pa) in the management of acute ischemic stroke is the accepted standard of practice. current guidelines for intravenous (iv) t-pa therapy in acute ischemic stroke do not exclude patients with infective endocarditis. we present three patients who received iv t-pa for acute ischemic stroke in the setting of infective endocarditis and ... | 2010 | 19688612 |
| the effect of argr-dna binding affinity on ornithine production in corynebacterium glutamicum. | pembtl-sy1, which can over produce the argr protein in corynebacterium glutamicum, was constructed. the dna-binding affinity of argr was analyzed using a chromatin immunoprecipitation (chip) assay. the level of argr protein expression in the plasmid-carrying c. glutamicum (pembtl-sy1) was higher than that in the wild-type strain. on the other hand, there was no increase in the dna-binding affinity of argr on the upstream of argb and the level of ornithine production. the dna-binding affinity of ... | 2009 | 19688381 |
| development of an unsteady-state model for a biological system in miniaturized bioreactors. | in the present study, the special shake flasks, so-called ventilation flasks, are equipped with oxygen sensors and then an unsteady-state gas transfer model for shake flasks was developed and experimentally investigated for a wide range of gas transfer resistances (kplug). for the validation of our unsteady-state model to simulate the gas transfer in a biological system in the ventilation flasks, a strain of corenobacterium glutamicum dm1730 was used as a model organism. for further easy process ... | 2009 | 19681756 |
| treatment of otitis externa associated with corynebacterium kroppenstedtii in a peach-faced lovebird (agapornis roseicollis) with an acetic and boric acid commercial solution. | a 5-year-old lovebird (agapornis roseicollis) was presented with scaly crusts around both external ear openings and exudate present around the left ear. the bird had been treated with ivermectin and enrofloxacin without success. a pure culture of corynebacterium kroppenstedtii was isolated from both ears. after susceptibility testing, a treatment of an acetic and boric acid solution administered topically 3 times daily was prescribed. the scaly appearance disappeared after 14 days of treatment a ... | 2009 | 19673461 |
| corynebacterium pilbarense sp. nov., a non-lipophilic corynebacterium isolated from a human ankle aspirate. | a non-lipophilic coryneform bacterium isolated from an anaerobic bactec bottle inoculated with an ankle aspirate from a male patient was characterized by phenotypic and molecular taxonomic methods. chemotaxonomic investigations revealed the presence of short-chain mycolic acids in the cell wall of the bacterium, a feature consistent with members of the genus corynebacterium. comparative 16s rrna gene sequence analysis demonstrated that the isolate displayed 92.0-99.0 % gene sequence similarity w ... | 2010 | 19671713 |
| distribution of bacterial keratitis and emerging resistance to antibiotics in china from 2001 to 2004. | to study on the distribution of bacterial keratitis isolates and the resistance to antibiotics in china from 2001 to 2004. | 2008 | 19668756 |
| vertebral osteomyelitis following vertebroplasty: is acne a potential contraindication and are prophylactic antibiotics mandatory prior to vertebroplasty? | we present a case of vertebral osteomyelitis following vertebroplasty in a patient with acne of the back. vertebral biopsy revealed corynebacterium species and blood cultures were positive for propionibacterium species. the patient did not respond to long-term intravenous antibiotics and required vertebral corpectomy and debridement with instrumentation. interventionalists should be able to recognize acne of the back and consider this as a potential contraindication to vertebroplasty. prophylact ... | 2009 | 19668286 |
| leifsonia soli sp. nov., a yellow-pigmented actinobacterium isolated from teak rhizosphere soil. | two yellow-pigmented, gram-stain-positive, aerobic, motile, short rod-shaped bacteria were isolated from natural teak tree rhizosphere soil and their taxonomic positions were determined by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strains tg-s248(t) and tg-s240 formed a distinct phyletic line within the genus leifsonia. 16s rrna gene sequence analysis of strain tg-s248(t) with sequences from leifsonia shinshuensis db 102(t), l. poae vkm ac-1401(t), l. n ... | 2010 | 19667370 |
| corynebacterium pyruviciproducens sp. nov., a pyruvic acid producer. | a coryneform strain, 06-1773o(t) (=wal 19168(t)), derived from a groin abscess sample was characterized using phenotypic and molecular taxonomic methods. comparative analyses revealed more than 3 % divergence of the 16s rrna gene sequence and about 10 % divergence of the partial rpob gene sequence from the type strain of corynebacterium glucuronolyticum. the strain could also be differentiated from c. glucuronolyticum by a set of phenotypic properties. a dna-dna relatedness study between strain ... | 2010 | 19666798 |
| the glxr regulon of the amino acid producer corynebacterium glutamicum: detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model. | corynebacterium glutamicum is an industrially important producer of amino acids and an emerging model system for the corynebacterineae. the glxr gene of c. glutamicum atcc 13032 encodes a dna binding transcription factor of the crp-fnr protein family. available data indicated a prominent role of glxr in the transcriptional regulatory network of c. glutamicum. we have used recently published whole-genome annotations of several corynebacteria to derive further evidence for glxr-mediated transcript ... | 2009 | 19665500 |
| high seroprevalence of caseous lymphadenitis in brazilian goat herds revealed by corynebacterium pseudotuberculosis secreted proteins-based elisa. | we conducted a seroepidemiological survey to determine the prevalence of caseous lymphadenitis (cla) in goat herds in minas gerais state, brazil. serum samples were collected from goats (n=676) from 108 rural properties in 2001, covering most of the sub-regions of this ca. 586,500 square kilometer state. antibodies against corynebacterium pseudotuberculosis secreted proteins were detected by an indirect enzyme-linked immunosorbent assay (elisa). most of the animals (78.9%) tested positive for cl ... | 2010 | 19665155 |
| assignment of brevibacterium stationis (zobell and upham 1944) breed 1953 to the genus corynebacterium, as corynebacterium stationis comb. nov., and emended description of the genus corynebacterium to include isolates that can alkalinize citrate. | brevibacterium stationis atcc 14403(t), corynebacterium ammoniagenes atcc 6872 and two clinical isolates were found to form a single taxon group consistent with the genus corynebacterium, designated here as corynebacterium stationis comb. nov. the type strain of corynebacterium stationis is atcc 14403(t) =ccug 43497( t) =cip 104228(t) =dsm 20302(t) =nbrc 12144(t) =jcm 11611(t) =vkm b-1228(t). these strains can utilize citrate; therefore, inclusion of c. stationis requires that the description of ... | 2010 | 19661509 |
| corynebacterium mustelae sp. nov., isolated from a ferret with lethal sepsis. | a non-lipophilic coryneform bacterium, strain 3105(t), was isolated from various tissues of a ferret with lethal sepsis. the strain was characterized by phenotypic and chemotaxonomic methods, which suggested an assignment of the isolate to the genus corynebacterium. strain 3105(t) exhibited the following peculiar features that made it possible to differentiate it phenotypically from all other corynebacteria: its distinctive 'humid cellar'-like odour, strong adherence to agar and a greenish-beige ... | 2010 | 19661496 |
| cutaneous manifestations of corynebacterium jeikeium sepsis. | corynebacterium jeikeium is a rare but increasingly important cause of septicemia in neutropenic patients that can present with characteristic cutaneous findings. a 61-year-old man with myelodysplastic syndrome developed c. jeikeium sepsis and an erythematous papular eruption while neutropenic from induction chemotherapy. a review of the english language literature shows only six reported cases of hemorrhagic or erythematous papular eruption in c. jeikeium sepsis. the case is presented with an u ... | 2009 | 19659870 |
| does the microbial flora in the ejaculate affect the freezeability of stallion sperm? | in an attempt to evaluate the possible relationship between the microbial flora in the stallion ejaculate and its ability to freeze,three ejaculates from five stallions were frozen using a standard protocol. before freezing, an aliquot was removed for bacteriological analysis. bacterial growth was observed in all the ejaculates studied. the isolated microorganisms were:staphylococcus spp. and micrococcus spp. (in all the stallions), beta-haemolytic streptococcus (in stallions 3 and 4), corynebac ... | 2009 | 19655428 |
| aftd, a novel essential arabinofuranosyltransferase from mycobacteria. | arabinogalactan (ag) and lipoarabinomannan (lam) are the two major cell wall (lipo)polysaccharides of mycobacteria. they share arabinan chains made of linear segments of alpha-1,5-linked d-araf residues with some alpha-1,3-branching, the biosynthesis of which offers opportunities for new chemotherapeutics. in search of the missing arabinofuranosyltransferases (arats) responsible for the formation of the arabinan domains of ag and lam in mycobacterium tuberculosis, we identified rv0236c (aftd) as ... | 2009 | 19654261 |
| expression, purification, crystallization and preliminary crystallographic analysis of spaa, a major pilin from corynebacterium diphtheriae. | bacterial pili are cell-surface organelles that are critically involved in adhesion to host cells, leading to the colonization of host tissues and the establishment of infections. whereas the pili of gram-negative bacteria have been extensively studied, those of gram-positive bacteria came to light only recently after the discovery and characterization of corynebacterium diphtheriae pili. these newly discovered pili are formed by the covalent polymerization of pilin subunits catalyzed by sortase ... | 2009 | 19652344 |
| development and experimental verification of a genome-scale metabolic model for corynebacterium glutamicum. | abstract: | 2009 | 19646286 |
| a possibilistic framework for constraint-based metabolic flux analysis. | constraint-based models allow the calculation of the metabolic flux states that can be exhibited by cells, standing out as a powerful analytical tool, but they do not determine which of these are likely to be existing under given circumstances. typical methods to perform these predictions are (a) flux balance analysis, which is based on the assumption that cell behaviour is optimal, and (b) metabolic flux analysis, which combines the model with experimental measurements. | 2009 | 19646223 |
| hoyosella altamirensis gen. nov., sp. nov., a new member of the order actinomycetales isolated from a cave biofilm. | a novel actinomycete, strain ofn s31(t), was isolated from a complex biofilm in the altamira cave, spain. a polyphasic study was carried out to clarify the taxonomic position of this strain. phylogenetic analysis with 16s rrna gene sequences of representatives of the genera corynebacterium, dietzia, gordonia, millisia, mycobacterium, nocardia, rhodococcus, segniliparus, skermania, tsukamurella and williamsia indicated that strain ofn s31(t) formed a distinct taxon in the 16s rrna gene tree that ... | 2009 | 19643882 |
| comparison between waste frying oil and paraffin as carbon source in the production of biodemulsifier by dietzia sp. s-js-1. | in order to lower the production cost, waste frying oils were used in the biosynthesis of demulsifier by dietzia sp. s-js-1, which was isolated from petroleum contaminated soil. after 7 days of cultivation, the biomass concentration of the most suitable waste frying oil (wfo ii) culture reached 3.78 g/l, which was 2.4 times the concentration of paraffin culture. the biodemulsifier produced with wfo ii culture broke the emulsions more efficiently than that produced with paraffin culture, given th ... | 2009 | 19643603 |
| in vivo imaging of s-layer nanoarrays on corynebacterium glutamicum. | crystalline bacterial cell surface layers (s-layers) are monomolecular arrays of (glyco)proteins that have recently produced a wealth of new opportunities in nanotechnology. whereas the in vitro imaging of isolated s-layers is well established, their direct imaging on live cells remains very challenging. here we use atomic force microscopy (afm) to visualize s-layer nanoarrays on living corynebacterium glutamicum bacteria. we demonstrate the presence of two highly ordered surface layers. the mos ... | 2009 | 19642621 |
| first description of nod2 variant associated with defective neutrophil responses in a woman with granulomatous mastitis related to corynebacteria. | we report the first case of granulomatous mastitis due to corynebacterium kroppenstedtii linked to strongly impaired neutrophil responses to nod2 agonist and a single nucleotide polymorphism within the nod2 gene (snp13 [leu1007fsinsc]) in a heterozygous state. these findings provided the first demonstration of impaired nod2 function associated with corynebacterial infection. | 2009 | 19641059 |
| physiological adaptation of corynebacterium glutamicum to benzoate as alternative carbon source - a membrane proteome-centric view. | the ability of microorganisms to assimilate aromatic substances as alternative carbon sources is the basis of biodegradation of natural as well as industrial aromatic compounds. in this study, corynebacterium glutamicum was grown on benzoate as sole carbon and energy source. to extend the scarce knowledge about physiological adaptation processes occurring in this cell compartment, the membrane proteome was investigated under quantitative and qualitative aspects by applying shotgun proteomics to ... | 2009 | 19639586 |
| nmr assignments of cd-ho, a 24 kda heme oxygenase from corynebacterium diphtheria. | we are employing a number of selective in vitro and in vivo methods including nmr to screen compounds that bind to heme oxygenases from pathogenic bacteria. we report the nearly complete hn, n, co, calpha and cbeta chemical shift assignments of a 215-amino acid ho from corynebacterium diphtheria in three forms, apo cd-ho-g135a, apo cd-ho and co-bound ferrous holo cd-ho; these assignments will enable us to identify residues on cd-ho that are perturbed upon binding to selected compounds, and to he ... | 2007 | 19636825 |
| endophytic bacteria associated with growing shoot tips of banana (musa sp.) cv. grand naine and the affinity of endophytes to the host. | a cultivation-based assessment of endophytic bacteria present in deep-seated shoot tips of banana suckers was made with a view to generate information on the associated organisms, potential endophytic contaminants in tissue-cultured bananas and to assess if the endophytes shared a beneficial relationship with the host. plating the tissue homogenate from the central core of suckers showed colony growth on nutrient agar from just 75% and 42% of the 12 stocks during may and november, respectively ( ... | 2009 | 19633807 |
| molecular characterization and therapeutic potential of a marine bacterium pseudoalteromonas sp. kmm 701 alpha-galactosidase. | an alpha-galactosidase capable of converting b red blood cells into the universal blood type cells at the neutral ph was produced by a novel obligate marine bacterium strain kmm 701 (vkm b-2135 d). the organism is heterotrophic, aerobic, and halophilic and requires na+ ions and temperature up to 34 degrees c for its growth. the strain has a unique combination of polysaccharide-degrading enzymes. its single intracellular alpha-galactosidase exceeded other glycoside hydrolases in the level of expr ... | 2010 | 19629597 |
| a case of afebrile pneumonia caused by non-toxigenic corynebacterium diphtheriae. | 2009 | 19628919 | |
| corynebacterium diphtheriae as an emerging pathogen in nephrostomy catheter-related infection: evaluation of traits associated with bacterial virulence. | corynebacterium diphtheriae still represents a global medical challenge, particularly due to the significant number of individuals susceptible to diphtheria and the emergence of non-toxigenic strains as the causative agents of invasive infections. in this study, we characterized the clinical and microbiological features of what we believe to be the first case of c. diphtheriae infection of a percutaneous nephrostomy catheter insertion site in an elderly patient with a fatal bladder cancer. moreo ... | 2009 | 19628642 |
| identification of a second beta-glucoside phosphoenolpyruvate: carbohydrate phosphotransferase system in corynebacterium glutamicum r. | the phosphoenolpyruvate : carbohydrate phosphotransferase system (pts) catalyses carbohydrate transport by coupling it to phosphorylation. previously, we reported a corynebacterium glutamicum r beta-glucoside pts encoded by bglf. here we report that c. glutamicum r contains an additional beta-glucoside pts gene, bglf2, organized in a cluster with a putative phospho-beta-glucosidase gene, bgla2, and a putative antiterminator, bglg2. while single gene disruption strains of either bglf or bglf2 wer ... | 2009 | 19628558 |
| dna binding by corynebacterium glutamicum tetr-type transcription regulator amtr. | the tetr family member amtr is the central regulator of nitrogen starvation response in corynebacterium glutamicum. while the amtr regulon was physiologically characterized in great detail up to now, mechanistic questions of amtr binding were not addressed. this study presents a characterization of functionally important amino acids in the dna binding domain of amtr and of crucial nucleotides in the amtr recognition motif. | 2009 | 19627583 |
| corynebacterium doosanense sp. nov., isolated from activated sludge. | the taxonomic position of a gram-positive, non-motile, non-spore-forming coryneform, isolated from activated sludge and designated strain cau 212(t), was investigated using a polyphasic approach. cellular morphology, biochemical tests and chemotaxonomic investigations revealed that strain cau 212(t) had the characteristics of the genus corynebacterium. comparative 16s rrna gene sequence analysis showed that the organism formed a hitherto-unknown subline within the genus corynebacterium. sequence ... | 2009 | 19625432 |
| heat-killed propionibacterium acnes is capable of inducing inflammatory responses in skin. | the etiology of acne is a complex process, and acne is one of the most common skin disorders affecting millions of people. the pathogenesis of acne is closely associated with the bacterium, propionibacterium acnes which was previously known as corynebacterium parvum. both viable and non-viable p. acnes/c. parvum have been shown to induce an immunostimulatory effect in vivo, suggesting that even dead bacteria continue to activate an inflammatory response. acne treatments with lasers or devices, i ... | 2009 | 19624731 |
| [features of biologic characteristics of corynebacterium diphtheriae circulating in postepidemic period]. | to study main biologic characteristics of c. diphtheriae strains circulating in north-west region of russia for the last 15 years. | 2009 | 19623714 |
| corynebacterium maris sp. nov., a marine bacterium isolated from the mucus of the coral fungia granulosa. | a bacterial strain, designated coryn-1(t), was isolated from mucus of the coral fungia granulosa (northern red sea, gulf of eilat, israel) by growth and enrichment of micro-organisms in agar spheres and subsequent plating. the bacterium was found to be a gram-positive, non-motile, halotolerant, heterotrophic coccobacillus. comparative 16s rrna gene sequence analyses showed that strain coryn-1(t) belonged to the genus corynebacterium, exhibiting the highest levels of similarity (94 %) with the 16 ... | 2009 | 19622641 |
| [infective endocarditis in patients with chronic hepatic failure: a four cases series]. | infective endocarditis and liver cirrhosis is an infrequent association. a retrospective study was performed in order to characterize predisposing factors, microbial causes and evolution. medical records between 1995 and 2008 (june) were searched. four cases were identified. in three cases liver cirrhosis was in stage child a, and in all 4 there was a predisposing cardiac disease. clinical manifestations were classical in 3 cases and in one presented as hepatic failure. only in one case a typica ... | 2009 | 19621160 |
| a method for enzyme quenching in microbial metabolome analysis successfully applied to gram-positive and gram-negative bacteria and yeast. | although microbial metabolome analysis has now become a widely used method, no generally applicable quenching method has been published so far. either the methods were established for only one defined organism or the metabolite coverage was quite low. in the current work, a novel, reliable, and robust quenching method for different types of organisms is described. compared with the commonly used quenching procedure with 60% methanol (-50 degrees c), we obtained improved results for three examine ... | 2009 | 19615328 |
| corynebacterium freiburgense sp. nov., isolated from a wound obtained from a dog bite. | a non-lipophilic, coryneform bacterium, isolated from a patient's wound obtained from a dog bite, was characterized by phenotypic, chemotaxonomic and molecular genetic methods. chemotaxonomic features suggested assignment of the unknown bacterium to the genus corynebacterium. the isolate exhibited the following peculiar features which made it possible to differentiate it phenotypically from all other medically relevant corynebacteria: older colonies exhibited a 'spoke-wheel' macroscopic morpholo ... | 2009 | 19605716 |
| identification and characterization of antimicrobial peptide, defensin, in the taiga tick, ixodes persulcatus. | ixodes persulcatus is the primary vector for human tick-borne diseases in japan. a cdna library was constructed from whole body homogenates of fed nymphs of i. persulcatus. from this library, one cdna encoding defensin-like antimicrobial peptide was identified. the amino-acid sequence showed high similarity to those of the defensins of other ticks and arthropods. i. persulcatus defensin mrna transcripts were detected at all life cycle stages of fed ticks and found to be predominantly expressed i ... | 2009 | 19604312 |
| acyl enzyme intermediates in sortase-catalyzed pilus morphogenesis in gram-positive bacteria. | in gram-positive bacteria, covalently linked pilus polymers are assembled by a specific transpeptidase enzyme called pilus-specific sortase. this sortase is postulated to cleave the lpxtg motif of a pilin precursor between threonine and glycine and to form an acyl enzyme intermediate with the substrate. pilus polymerization is believed to occur through the resolution of this intermediate upon specific nucleophilic attack by the conserved lysine located within the pilin motif of another pilin mon ... | 2009 | 19592583 |
| genetic and biochemical identification of the chorismate mutase from corynebacterium glutamicum. | chorismate mutase (cm) catalyses the rearrangement of chorismate to prephenate and is also the first and the key enzyme that diverges the shikimate pathway to either tryptophan (trp) or phenylalanine (phe) and tyrosine (tyr). corynebacterium glutamicum is one of the most important amino acid producers for the fermentation industry and has been widely investigated. however, the gene(s) encoding cm has not been experimentally identified in c. glutamicum. in this study, the ncgl0819 gene, which was ... | 2009 | 19589834 |
| transcriptional control of the succinate dehydrogenase operon sdhcab of corynebacterium glutamicum by the camp-dependent regulator glxr and the luxr-type regulator rama. | in experiments performed to identify transcriptional regulators of the tricarboxylic acid cycle of corynebacterium glutamicum, the camp-dependent regulator glxr and the regulators of acetate metabolism rama and ramb were enriched by dna affinity chromatography with the promoter region of the sdhcab operon encoding succinate dehydrogenase. the binding of purified glxr, rama and ramb was verified by electrophoretic mobility shift assays and the regulatory effects of these proteins on sdhcab gene e ... | 2009 | 19583988 |
| characterization of the dicarboxylate transporter dcta in corynebacterium glutamicum. | transporters of the dicarboxylate amino acid-cation symporter family often mediate uptake of c(4)-dicarboxylates, such as succinate or l-malate, in bacteria. a member of this family, dicarboxylate transporter a (dcta) from corynebacterium glutamicum, was characterized to catalyze uptake of the c(4)-dicarboxylates succinate, fumarate, and l-malate, which was inhibited by oxaloacetate, 2-oxoglutarate, and glyoxylate. dcta activity was not affected by sodium availability but was dependent on the el ... | 2009 | 19581365 |
| sequence-specific bacterial growth inhibition by peptide nucleic acid targeted to the mrna binding site of 16s rrna. | peptide nucleic acid (pna) targeted to the functional domains of 23s rrna can inhibit translation and cell growth. however, effective inhibition of translation and cell growth using 16s rrna-targeted pna has still not been achieved. here, we report that pna targeted to the functional site of 16s rrna could inhibit both gene expression in vitro and bacterial growth in pure culture with sequence specificity. we used 10-mer pnas conjugated with a cell-penetrating peptide, which targeted the mrna bi ... | 2009 | 19578844 |
| corynebacterium peritonitis in australian peritoneal dialysis patients: predictors, treatment and outcomes in 82 cases. | infection due to corynebacterium species has been reported with increasing frequency over recent decades. the impacts of enhanced laboratory detection together with widespread use of new peritoneal dialysis (pd) connection technology and antimicrobial prophylaxis strategies on corynebacterium pd-associated peritonitis have not been well studied. | 2009 | 19574339 |
| [etiological analysis of ocular non-viral microbial isolates in children and their antibiotics susceptibility in vitro]. | to analyze the types, distribution characteristics and in vitro susceptibility of antibiotic agents of ocular non-viral microbial isolates in children. | 2009 | 19573335 |
| characterization of an adenylate cyclase gene (cyab) deletion mutant of corynebacterium glutamicum atcc 13032. | genome analysis of c. glutamicum atcc 13032 has showed one putative adenylate cyclase gene, cyab (cg0375) which encodes membrane protein belonging to class iii adenylate cyclases. to characterize the function of cyab, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic amp compared to that of wild-type. interestingly, the cyab mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyab gene. similarly, i ... | 2010 | 19568747 |
| corynebacterium timonense sp. nov. and corynebacterium massiliense sp. nov., isolated from human blood and human articular hip fluid. | gram-positive, facultatively anaerobic, rod-shaped bacteria were isolated from the blood of a patient with endocarditis (strain 5401744t) and from the hip joint fluid of a patient with an infected orthopaedic prosthesis (strain 5402485t). these strains were characterized by using a polyphasic taxonomic approach. based on cellular morphology and biochemical criteria the two isolates were tentatively assigned to the genus corynebacterium, although they did not correspond to any recognized species. ... | 2009 | 19567562 |
| structure and function of a complex between chorismate mutase and dahp synthase: efficiency boost for the junior partner. | chorismate mutase catalyzes a key step in the shikimate biosynthetic pathway towards phenylalanine and tyrosine. curiously, the intracellular chorismate mutase of mycobacterium tuberculosis (mtcm; rv0948c) has poor activity and lacks prominent active-site residues. however, its catalytic efficiency increases >100-fold on addition of dahp synthase (mtds; rv2178c), another shikimate-pathway enzyme. the 2.35 a crystal structure of the mtcm-mtds complex bound to a transition-state analogue shows a c ... | 2009 | 19556970 |
| efficacy of diphtheria and tetanus vaccination in gaza, palestine. | this study evaluated the effectiveness and usefulness of vaccination against diphtheria and tetanus in different age groups in gaza, palestine. blood samples were collected from 180 children aged <12 years, 90 males and 90 females. using elisa methods, the efficacy of vaccination was estimated at 87.8% for diphtheria and 98.3% for tetanus. mean serum titres varied significantly by age group: for diphtheria 0.24 iu/ml at age 2-4 years, 0.63 iu/ml at 7-8 years and 0.46 iu/ml at 11-12 years, and fo ... | 2009 | 19554974 |
| diviva uses an n-terminal conserved region and two coiled-coil domains to localize and sustain the polar growth in corynebacterium glutamicum. | corynebacterium glutamicum is a rod-shaped actinomycete with a distinct model of peptidoglycan synthesis during cell elongation, which takes place at the cell poles and is sustained by the essential protein diviva(cg) (c. glutamicum diviva). this protein contains a short conserved n-terminal domain and two coiled-coil regions: cc1 and cc2. domain deletions and chimeric versions of diviva were used to functionally characterize the three domains, and all three were found to be essential for proper ... | 2009 | 19552709 |
| quantitative high-throughput screening identifies inhibitors of anthrax-induced cell death. | here, we report the results of a quantitative high-throughput screen (qhts) measuring the endocytosis and translocation of a beta-lactamase-fused-lethal factor and the identification of small molecules capable of obstructing the process of anthrax toxin internalization. several small molecules protect raw264.7 macrophages and cho cells from anthrax lethal toxin and protected cells from an lf-pseudomonas exotoxin fusion protein and diphtheria toxin. further efforts demonstrated that these compoun ... | 2009 | 19540764 |
| biodegradation of phenol using corynebacterium sp. dj1 aerobic granules. | the single-culture corynebacterium sp. dj1 aerobic granules were cultivated and were utilized to degrade high-strength phenolic wastewater. these granules can degrade phenol at sufficient high rate without severe inhibitory effects up to phenol concentration of 2000 mg l(-1). furthermore, the kinetic characteristic noted for these granules yields a zero-order phenol degradation behavior with 500-1500 mg l(-1) phenol, which facilitates reactor design and scale up. with added acetate to promote ce ... | 2009 | 19540750 |
| engineering of pentose transport in corynebacterium glutamicum to improve simultaneous utilization of mixed sugars. | corynebacterium glutamicum strains cra1 and crx2 are able to grow on l-arabinose and d-xylose, respectively, as sole carbon sources. nevertheless, they exhibit the major shortcoming that their sugar consumption appreciably declines at lower concentrations of these substrates. to address this, the c. glutamicum atcc31831 l-arabinose transporter gene, arae, was independently integrated into both strains. unlike its parental strain, resultant cra1-arae was able to aerobically grow at low (3.6 g.l(- ... | 2009 | 19529932 |
| detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in hungary. | the drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific pcrs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. the results obtained verify the higher sensitivity of pcr compared to cultivation for detecting legionella and pseudomonas aeruginosa. moreover, several other opportunistic pathogenic bacteria, such as escherichia albertii, acinetobacter lwoffi and corynebacterium tuberculos ... | 2010 | 19519854 |
| structural and phylogenetic analysis of a conserved actinobacteria-specific protein (asp1; sco1997) from streptomyces coelicolor. | the actinobacteria phylum represents one of the largest and most diverse groups of bacteria, encompassing many important and well-characterized organisms including streptomyces, bifidobacterium, corynebacterium and mycobacterium. members of this phylum are remarkably diverse in terms of life cycle, morphology, physiology and ecology. recent comparative genomic analysis of 19 actinobacterial species determined that only 5 genes of unknown function uniquely define this large phylum 1. the cellular ... | 2009 | 19515238 |
| bacteriology of infected extracted pacemaker and icd leads. | pacemaker and implantable cardioverter defibrillator infections, when not treated, lead to serious consequences. the aim is to identify the prevalent strains of the responsible bacteria to guide an effective therapy. | 2009 | 19512941 |
| siccanin rediscovered as a species-selective succinate dehydrogenase inhibitor. | to identify antibiotics targeting to respiratory enzymes, we carried out matrix screening of a structurally varied natural compound library with pseudomonas aeruginosa membrane-bound respiratory enzymes. we identified a succinate dehydrogenase inhibitor, siccanin (ic(50), 0.9 microm), which is a potent antibiotic against some pathogenic fungi like trichophyton mentagrophytes and inhibits their mitochondrial succinate dehydrogenase. we found that siccanin was effective against enzymes from p. aer ... | 2009 | 19505951 |
| heme-dependent autophosphorylation of a heme sensor kinase, chrs, from corynebacterium diphtheriae reconstituted in proteoliposomes. | corynebacterium diphteriae employs the response regulator, chra, and the sensor kinase, chrs, of a two-component signal transduction system to utilize host heme iron. although chrs is predicted to encode a heme sensor, the sensing mechanism remains to be characterized. in this report, chrs expressed in eshcherichia coli membranes was solubilized and purified using decylmaltoside. chrs protein incorporated into proteoliposomes catalyzed heme-dependent autophosphorylation by atp. other metalloporp ... | 2009 | 19505463 |
| integrated analysis and reconstruction of microbial transcriptional gene regulatory networks using coryneregnet. | coryneregnet is the reference database and analysis platform for corynebacterial gene regulatory networks. it provides web-based access to integrated data on gene regulatory interactions of corynebacteria relevant to human medicine and biotechnology, escherichia coli and mycobacterium tuberculosis. to facilitate the analysis and reconstruction of the corresponding networks, coryneregnet provides user-friendly interfaces for bioinformatics analysis and network visualization tools. this protocol d ... | 2009 | 19498379 |
| from corynebacterium glutamicum to mycobacterium tuberculosis--towards transfers of gene regulatory networks and integrated data analyses with mycoregnet. | year by year, approximately two million people die from tuberculosis, a disease caused by the bacterium mycobacterium tuberculosis. there is a tremendous need for new anti-tuberculosis therapies (antituberculotica) and drugs to cope with the spread of tuberculosis. despite many efforts to obtain a better understanding of m. tuberculosis' pathogenicity and its survival strategy in humans, many questions are still unresolved. among other cellular processes in bacteria, pathogenicity is controlled ... | 2009 | 19494184 |
| properties of arsenite efflux permeases (acr3) from alkaliphilus metalliredigens and corynebacterium glutamicum. | members of the acr3 family of arsenite permeases confer resistance to trivalent arsenic by extrusion from cells, with members in every phylogenetic domain. in this study bacterial acr3 homologues from alkaliphilus metalliredigens and corynebacterium glutamicum were cloned and expressed in escherichia coli. modification of a single cysteine residue that is conserved in all analyzed acr3 homologues resulted in loss of transport activity, indicating that it plays a role in acr3 function. the result ... | 2009 | 19494117 |
| crystal structures of the regulatory subunit of thr-sensitive aspartate kinase from thermus thermophilus. | crystal structures of the regulatory subunit of thr-sensitive aspartate kinase (ak; ec 2.7.2.4) from thermus thermophilus (ttakbeta) were determined at 2.15 a in the thr-bound form (ttakbeta-thr) and at 2.98 a in the thr-free form (ttakbeta-free). although both forms are crystallized as dimers, the contact surface area of the dimer interface in ttakbeta-free (3200 a(2)) is smaller than that of ttakbeta-thr (3890 a(2)). sedimentation equilibrium analyzed by ultracentrifugation revealed that ttakb ... | 2009 | 19490113 |
| [an eight-year review of sensitivity to antimicrobials against isolated microorganisms from ocular infections]. | to obtain data beneficial for future treatment by observing the circumstances relating to microorganisms isolated in external ocular infections and drug sensitivity, along with changes in drug-resistant bacteria. | 2009 | 19489449 |
| hyastatin, a glycine-rich multi-domain antimicrobial peptide isolated from the spider crab (hyas araneus) hemocytes. | marine invertebrates are a rich source for the discovery of novel antimicrobial peptides, compounds regarded as important defense components in the host defense system. here we report the purification and characterization of an 11.7kda gly-rich peptide, named hyastatin, from the hemocytes of hyas araneus. it consists of three distinctly different domains: an n-terminal region enriched in gly residues, a short pro/arg-rich region, and a c-terminal region containing six cys residues with a cys pat ... | 2009 | 19487032 |
| identification of a terminal rhamnopyranosyltransferase (rpta) involved in corynebacterium glutamicum cell wall biosynthesis. | a bioinformatics approach identified a putative integral membrane protein, ncgl0543, in corynebacterium glutamicum, with 13 predicted transmembrane domains and a glycosyltransferase motif (rxxde), features that are common to the glycosyltransferase c superfamily of glycosyltransferases. the deletion of c. glutamicum ncgl0543 resulted in a viable mutant. further glycosyl linkage analyses of the mycolyl-arabinogalactan-peptidoglycan complex revealed a reduction of terminal rhamnopyranosyl-linked r ... | 2009 | 19482925 |
| distribution characteristics of airborne bacteria and fungi in the feedstuff-manufacturing factories. | the objective of the study is to investigate the distribution patterns of airborne bacteria and fungi in three feedstuff-manufacturing factories in korea. the geometric mean (+/-gsd) levels of airborne bacteria and fungi in the feedstuff-manufacturing factory were 113(+/-18)cfu/m(3) and 89(+/-5)cfu/m(3) for pelleting process and 198(+/-5)cfu/m(3) and 124(+/-12)cfu/m(3) for powdering process, respectively. the percentage of respirable (0.65-4.7 microm) and total (>4.7 microm) concentration of air ... | 2009 | 19481347 |
| antimicrobial activity of six pomegranate (punica granatum l.) varieties and their relation to some of their pomological and phytonutrient characteristics. | arils from six pomegranate (punica granatum l.) varieties grown in the mediterranean region of turkey were tested for their antimicrobial properties by the agar diffusion and minimum inhibitory concentration (mic) methods against seven bacteria: (bacillus megaterium dsm 32, pseudomonas aeruginosa dsm 9027, staphylococcus aureus cowan 1, corynebacterium xerosis uc 9165, escherichia coli dm, enterococcus faecalis a10, micrococcus luteus la 2971), and threefungi (kluvyeromyces marxianus a230, rhodo ... | 2009 | 19471201 |
| antibacterial study of the medium chain fatty acids and their 1-monoglycerides: individual effects and synergistic relationships. | the antibacterial activity of the medium chain fatty acids and their 1-monoglycerides was evaluated towards several gram-positive strains belonging to the genera staphylococcus, corynebacterium, bacillus, listeria and streptococcus. the 1-monoglycerides were more active than the fatty acids with monolaurin being the most active compound. interesting effects were observed when the streptococcal strain streptococcus pyogenes was used as a test microorganism. first, blocking of the hydroxyl groups ... | 2009 | 19469285 |
| genetic modification of flux for flux prediction of mutants. | gene deletion and overexpression are critical technologies for designing or improving the metabolic flux distribution of microbes. some algorithms including flux balance analysis (fba) and minimization of metabolic adjustment (moma) predict a flux distribution from a stoichiometric matrix in the mutants in which some metabolic genes are deleted or non-functional, but there are few algorithms that predict how a broad range of genetic modifications, such as over- and underexpression of metabolic g ... | 2009 | 19468056 |
| generation of plant-derived recombinant dtp subunit vaccine. | the current diphtheria-tetanus-pertussis (dtp) pediatric vaccine is produced from the corresponding pathogenic bacteria corynebacterium diphtheriae, clostridium tetani and bordetella pertussis; five injected doses of dtap (acellular) vaccine are required for every child in the standard us vaccination schedule. because the vaccine is derived from native live sources, adverse effects are possible and production is complex and costly. to address issues of safety, ease of renewability and expense, w ... | 2009 | 19464556 |
| [influence of polyoxydonium on adhesive properties of corynebacterium diphtheriae]. | to study the ability of polyoxydonium (po) to influence on adhesive properties of c. diphtheriae. | 2009 | 19459470 |
| microflora of the penile skin-lined neovagina of transsexual women. | the microflora of the penile skin-lined neovagina in male-to-female transsexuals is a recently created microbial niche which thus far has been characterized only to a very limited extent. yet the knowledge of this microflora can be considered as essential to the follow-up of transsexual women. the primary objective of this study was to map the neo-vaginal microflora in a group of 50 transsexual women for whom a neovagina was constructed by means of the inverted penile skin flap technique. second ... | 2009 | 19457233 |
| phenotypic differences between coryneform bacteria isolated from seminal fluid of healthy men and men with chronic prostatitis syndrome. | we compared the potential phenotypic properties of coryneform bacteria associated with chronic prostatitis syndrome (cps), such as secretory inhibitor of lysozyme (sil) and secretory inhibitor of platelet microbicidal protein (sipmp). a total of 110 clinical isolates of coryneform bacteria isolated from the seminal fluid of healthy men and men with cps were tested. sipmp production was tested by inhibiting platelet microbicidal protein (pmp) bioactivity against bacillus subtilis, and was express ... | 2009 | 19448644 |
| real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. | in more than 30% of milk samples from clinical and subclinical bovine mastitis, bacteria fail to grow even after 48 h of conventional culture. the "no-growth" samples are problematic for mastitis laboratories, veterinarians, and dairy producers. this study provides the first investigation of the bacteriological etiology of such samples, using a real-time pcr-based commercial reagent kit. the assay targets the dna of the 11 most common bacterial species or groups in mastitis and the staphylococca ... | 2009 | 19447993 |
| a cell-based screening system for detection of inhibitors toward mycobacterial cell wall core. | mycobacterium tuberculosis and nonpathogenic bacteria, corynebacterium glutamicum, possess a common and unusual cell wall architecture. a cell-based screening system was designed to identify novel compounds interacting with the synthesis, assembly or regulation of the m. tuberculosis cell wall. c. glutamicum was tested in a paired medium assay in 96-well plates with natural product extracts and pure chemical compounds in the presence and absence of the osmotic stabilizer, sorbitol and some ions. ... | 2009 | 19444301 |
| identification of a cell-wall channel in the corynemycolic acid-free gram-positive bacterium corynebacterium amycolatum. | as part of a comparative study of the cell wall of corynebacteria, a channel-forming protein was characterized in corynebacterium amycolatum, a species devoid of corynemycolic acids. corynebacterium amycolatum cells were disrupted and the cell envelope subjected to two different separation procedures, differential centrifugation to separate the different fractions of the cell envelope, and sucrose-step-gradient density centrifugation. the fractions obtained by the two methods were analyzed for l ... | 2009 | 19440981 |
| decreased sensitivity to changes in the concentration of metal ions as the basis for the hyperactivity of dtxr(e175k). | the metal-ion-activated diphtheria toxin repressor (dtxr) is responsible for the regulation of virulence and other genes in corynebacterium diphtheriae. a single point mutation in dtxr, dtxr(e175k), causes this mutant repressor to have a hyperactive phenotype. mice infected with mycobacterium tuberculosis transformed with plasmids carrying this mutant gene show reduced signs of the tuberculosis infection. corynebacterial dtxr is able to complement mycobacterial ider and vice versa. to date, an e ... | 2009 | 19433095 |
| development of novel cell surface display in corynebacterium glutamicum using porin. | we have developed a novel cell surface display in corynebacterium glutamicum using porin proteins as anchor proteins. porins are localized at c. glutamicum mycolic acid layer and exist as a hexamer. we used alpha-amylase from streptococcus bovis 148 (amya) as a model protein to be displayed on the c. glutamicum cell surface. amya was fused to the c terminus of the porins porb, porc, or porh. expression vectors using fused proteins under the control of the cspb promoter were constructed and intro ... | 2009 | 19430772 |
| the ldha gene, encoding fermentative l-lactate dehydrogenase of corynebacterium glutamicum, is under the control of positive feedback regulation mediated by lldr. | corynebacterium glutamicum ldha encodes l-lactate dehydrogenase, a key enzyme that couples l-lactate production to reoxidation of nadh formed during glycolysis. we previously showed that in the absence of sugar, sugr binds to the ldha promoter region, thereby repressing ldha expression. in this study we show that lldr is another protein that binds to the ldha promoter region, thus regulating ldha expression. lldr has hitherto been characterized as an l-lactate-responsive transcriptional represso ... | 2009 | 19429617 |
| regulative interactions of the osmosensing c-terminal domain in the trimeric glycine betaine transporter betp from corynebacterium glutamicum. | activation of the osmoregulated trimeric betaine transporter betp from corynebacterium glutamicum was shown to depend mainly on the correct folding and integrity of its 55 amino acid long, partly alpha-helical c-terminal domain. reorientation of the three c-terminal domains in the betp trimer indicates different lipid-protein and protein-protein interactions of the c-terminal domain during osmoregulation. a regulation mechanism is suggested where this domain switches the transporter from the ina ... | 2009 | 19426128 |
| role of il-10 deficiency in pneumonia induced by corynebacterium kutscheri in mice. | il-10 is an important anti-inflammatory cytokine that can inhibit the production of many pro-inflammatory cytokines. both human and animal studies have shown that pro-inflammatory cytokines play an important role in pneumonia and other inflammatory lung diseases. in the present study, il-10 knockout (ko) and wild-type mice were infected with corynebacterium kutscheri to determine whether the severity of pathogenesis and whether protective immunity could be altered in the absence of il- 10. the s ... | 2009 | 19421001 |
| life-threatening infection caused by daptomycin-resistant corynebacterium jeikeium in a neutropenic patient. | daptomycin is a novel lipopeptide antibiotic agent approved for the treatment of gram-positive life-threatening infections. here we report, for the first time, the isolation of a highly daptomycin-resistant strain of corynebacterium jeikeium causing a life-threatening infection in a neutropenic patient undergoing cord blood transplantation for secondary acute myeloid leukemia. | 2009 | 19420177 |
| growth and biosurfactant synthesis by nigerian hydrocarbon-degrading estuarine bacteria. | the ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. screening of hydrocarbon-utilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in nigeria, africa, resulted in the isolation of five microbial strains identified as corynebacterium sp. ddv1, flavobacterium sp. ddv2, micrococcus roseus ddv3, pseudomonas aeruginosa ddv4 and saccharomyces cere ... | 2008 | 19419068 |
| blood culture status in mature horses with diarrhoea: a possible association with survival. | the incidence and implications of positive blood cultures in mature horses with diarrhoea is unknown. the diagnosis of bacteraemia may alter treatment and prognosis. | 2009 | 19418745 |
| double deletion of dtsr1 and pyc induce efficient l: -glutamate overproduction in corynebacterium glutamicum. | corynebacterium glutamicum strains are used for the fermentative production of l-glutamate. five c. glutamicum deletion mutants were isolated by two rounds of selection for homologous recombination and identified by southern blot analysis. the growth, glucose consumption and glutamate production of the mutants were analyzed and compared with the wild-type atcc 13032 strain. double disruption of dtsr1 (encoding a subunit of acetyl-coa carboxylase complex) and pyc (encoding pyruvate carboxylase) c ... | 2009 | 19408028 |
| actinomyces hyovaginalis-associated lymphadenitis in a nubian goat. | a 6-year-old nubian goat with a history of progressive weight loss and cough was presented for necropsy. the goat tested negative for antibodies to caseous lymphadenitis and caprine arthritis and encephalitis by hemagglutination inhibition assay and enzyme-linked immunosorbent assay, respectively. postmortem examination revealed marked enlargement and, with histopathology, a fibrinopurulent necrotizing lymphadenitis of a tracheobronchial lymph node, with an appearance similar to that reported in ... | 2009 | 19407095 |
| corynebacterium lubricantis sp. nov., isolated from a coolant lubricant. | three gram-positive, rod-shaped, oxidase-negative, non-spore-forming, non-motile bacteria (kss-3se(t), kss-4se and kss-10se) were isolated from a coolant lubricant. 16s rrna gene sequence analyses revealed almost identical sequences, with only a few (<10 positions) differences for these three isolates. comparisons showed the highest similarities to corynebacterium pilosum nctc 11862(t) (97.6 % similarity with strain kss-3se(t)). similarities with other established corynebacterium species were lo ... | 2009 | 19406802 |
| comparison of three treatment regimens for sheep and goats with caseous lymphadenitis. | to compare the effectiveness of 3 treatment regimens for small ruminants with caseous lymphadenitis. | 2009 | 19405888 |
| the identification of enzyme targets for the optimization of a valine producing corynebacterium glutamicum strain using a kinetic model. | the enzyme targets for the rational optimization of a corynebacterium glutamicum strain constructed for valine production are identified by analyzing the control of flux in the valine/leucine pathway. the control analysis is based on measurements of the intracellular metabolite concentrations and on a kinetic model of the reactions in the investigated pathway. data-driven and model-based methods are used and evaluated against each other. the approach taken gives a quantitative evaluation of the ... | 2009 | 19405093 |
| indoor and outdoor airborne bacteria in child day-care centers in edirne city (turkey), seasonal distribution and influence of meteorological factors. | this paper presents information about airborne mesophilic bacteria in the indoor and outdoor air of child day-care centers (cdccs) in the city of edirne, turkey. air samples were collected using the petri plate gravitational settling method from the indoor and outdoor air of cdccs. counts of airborne bacteria were measured as colony forming units (cfu) collected by gravity onto brain heart infusion agar plates (with 5% sheep blood). samples were taken monthly over a period of 12 months between j ... | 2010 | 19404760 |
| regulation of quinone oxidoreductase by the redox-sensing transcriptional regulator qorr in corynebacterium glutamicum. | corynebacterium glutamicum cgr_1435 (cg1552) encodes a protein of the duf24 protein family, which is a novel family of transcriptional regulators. cgr1435 (qorr) is a negative regulator of cgr_1436 (qor2), which is located upstream of cgr_1435 (qorr) in the opposite orientation, and its structural gene. qorr binds to the intergenic region between qor2 and qorr to repress their expression, which is induced by the thiol-specific oxidant diamide. the dna-binding activity of qorr is impaired by oxid ... | 2009 | 19403527 |
| gluconate as suitable potential reduction supplier in corynebacterium glutamicum: cloning and expression of gntp and gntk in escherichia coli. | corynebacterium glutamicum is widely used in the industrial production of amino acids. we have found that this bacterium grows exponentially on a mineral medium supplemented with gluconate. gluconate permease and gluconokinase are expressed in an inducible form and, 6-phosphogluconate dehydrogenase, although constitutively expressed, shows a 3-fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions. interestingly, these activities are lower than ... | 2009 | 19399347 |
| productivity of cyclohexanone oxidation of the recombinant corynebacterium glutamicum expressing chnb of acinetobacter calcoaceticus. | the biocatalytic efficiency of recombinant corynebacterium glutamicum expressing the chnb gene encoding cyclohexanone monooxygenase (chmo) of acinetobacter calcoaceticus ncimb 9871 was investigated. optimization of an expression system and induction conditions enabled the recombinant biocatalyst to produce chmo to a specific activity of ca. 0.5 u mg(-1) protein. tight control of feeding of an energy source (i.e., glucose) and dissolved oxygen tension during fed-batch culture-based biotransformat ... | 2009 | 19397940 |