| antimicrobial activity of ro 23-9424, a novel ester-linked codrug of fleroxacin and desacetylcefotaxime. | ro 23-9424 is a novel ester-linked codrug of fleroxacin (ro 23-6240; am-833) and the cefotaxime metabolite desacetylcefotaxime. its potency was determined against over 1,000 organisms and found to be intermediate between those of the two components. more than 99% of members of the family enterobacteriaceae were inhibited by greater than or equal micrograms of ro 23-9424 per ml; its mic for 50% of strains tested ranged from greater than or equal to 0.06 to 1 micrograms/ml. staphylococci, streptoc ... | 1989 | 2504106 |
| structure determination of mycolic acids by using charge remote fragmentation. | the collision-induced remote site fragmentation process of closed-shell ions, such as carboxylate anions, is a very potent analytical tool for the structural determination of fatty acids. this leads to an easy location of branch points, double bonds, cyclopropane rings and other functional groups. although corynomycolic acid mixtures from corynebacterium diphtheriae can be directly analyzed by negative-ion fast atom bombardment combined with collisionally activated decomposition spectra, mycolic ... | 1989 | 2504502 |
| mycobacterium chelonei infection of a broviac catheter insertion site. | mycobacterium chelonei infection developed at the insertion site of an indwelling broviac catheter in a child with erythroleukemia. direct adherence to and colonization of the intra- and extra-luminal surfaces of the catheter, with extension to the adjacent subcutaneous tissue, by this rapidly growing mycobacterium may have been the primary factor underscoring the infection. nontuberculous mycobacteria such as mycobacterium chelonei grow readily on routine bacteriologic media and resemble coryne ... | 1989 | 2504592 |
| evidence for direct regulation of diphtheria toxin gene transcription by an fe2+-dependent dna-binding repressor, dtoxr, in corynebacterium diphtheriae. | previous studies provided indirect evidence that in corynebacterium diphtheriae regulation of diphtheria toxin gene (tox) transcription by iron is mediated by a bacterial repressor. by performing in vitro protein-dna binding experiments, we establish that a corynebacterial fe2+-sensitive protein, named dtoxr, can bind to a palindromic motif present in the tox promoter region. binding of this factor prevents the interaction of the transcription initiation machinery with presumptive critical promo ... | 1989 | 2506133 |
| in vitro evaluation of ramoplanin (a16686 or mdl62198). a new depsipeptide complex for potential topical use. | ramoplanin, a new depsipeptide complex, was found to be 4- to 8-fold more active than vancomycin against all gram-positive species (500 strains). similarly, ramoplanin was both more active and had an additional spectrum compared to mupirocin (formerly pseudomonic acid) against bacillus spp., corynebacterium jeikeium, enterococci and listeria monocytogenes. mupirocin-resistant staphylococcus spp. strains (less than 1%) were identified in these organisms collected from over 40 medical centers in t ... | 1989 | 2507218 |
| a superfamily of nadph-dependent reductases in eukaryotes and prokaryotes. | aldose reductase (ar) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. our studies are aimed at further clarifying the role of ar in diabetes and facilitating the design of new classes of potent, specific ar inhibitors by gaining an understanding of the protein structure of ar. to this end, we have determined the complete protein sequence of rat lens ar using cdna analysis and primer extension of mrna. by comparing protein sequenc ... | 1989 | 2507340 |
| [detection of toxin and evaluation of the degree of toxin-formation of corynebacterium diphtheriae using immunoenzyme analysis]. | toxin production and the intensity of toxin formation in 265 c. diphtheriae strains circulating in different areas of the ussr have been studied by the method of the enzyme-linked immunosorbent assay (elisa). this study has been carried out with the use of the assay system consisting of monoclonal antibodies to the cooh-area of the b-fragment of the toxin molecule adsorbed onto the surface of polystyrene plates, affinity-purified polyclonal antidiphtheria antibodies labeled with horse-radish per ... | 1989 | 2508377 |
| diphtheria in stockholm, with a theory concerning transmission. | since 1984, diphtheria has been diagnosed in sweden, mainly among men abusing alcohol. during the first half of 1986, eight cases of diphtheria were discovered in the city of stockholm. the first three were among men abusing alcohol. the following five cases had no connection with them. these five persons were employed in two companies housed in the same building. the only connection between them was that they ate their lunch at the same restaurant. one alcohol-abusing man had worked temporarily ... | 1989 | 2509569 |
| effect of oky-046 and ono-3708 on liver injury in mice. | the effects of oky-046, a selective thromboxane a2 (txa2) synthetase inhibitor, and ono-3708, a novel txa2 receptor antagonist, on liver disease were investigated in mice. the liver injury was induced by either an injection of antibasic liver protein (blp) antibody into dba/2 mice that had been previously immunized with rabbit igg or by an injection of bacterial lipopolysaccharide (lps) into corynebacterium parvum (c. parvum) pretreated ddy mice. 1) in both injury models, clear elevation of glut ... | 1989 | 2512404 |
| the deconjugation ability of bacteria isolated from the jejunal fluids in the blind loop syndrome with high 14co2 excretion--using the breath analysis technique and thin-layer chromatography. | five patients with blind loop syndrome (billroth ii) were examined by measuring 14co2 specific activity of expired breath samples taken at intervals after a meal containing glycine-1-14c cholate. the 5 patients tested showed a marked increase of 14co2 specific activity. furthermore, the ability of deconjugation of bacteria isolated from the jejunal fluids in the efferent loop of these patients was tested by thin layer chromatography. the bacterial species identified from the samples were as foll ... | 1989 | 2513463 |
| selective immunotoxins prepared with mutant diphtheria toxins coupled to monoclonal antibodies. | | 1989 | 2513466 |
| immunity against diphtheria in adults in poland. | the diphtheria immunity status was determined with the passive haemagglutination technique in 503 sera of 10-90-year-old persons from warsaw and olsztyn provinces. donors of sera were students, teachers, pregnant women, employees of industry and medical service. the immunity was highest (90% of titers 0.1 iu/ml or higher) in persons below 20 years of age and in persons above 60 years of age (55%). between these two groups, gaps in immunity exist, the proportion of those immune varying from 36-50 ... | 1989 | 2514113 |
| seroepidemiological survey of corynebacterium pseudotuberculosis infection in sheep in japan using enzyme-linked immunosorbent assay and immunodiffusion. | sera from 1186 apparently healthy sheep in hokkaido were examined by enzyme-linked immunosorbent assay (elisa) and immunodiffusion (id) for the presence of antibodies against corynebacterium pseudotuberculosis. elisa-positives were 466 (39.3%) while id-positives were 330 (27.8%). spread of caseous lymphadenitis in sheep in hokkaido was thus clarified. although id was less sensitive than elisa in detecting the antibodies against c. pseudotuberculosis it did not give any non-specific reaction. fro ... | 1989 | 2514314 |
| production of diphtheria toxin crm228 in b. subtilis. | the gene coding for a nontoxic diphtheria toxin (dt), tox228, was isolated from lysogenic corynebacterium diphtheriae and cloned into pbr322. a mature form of the tox228 gene, lacking its signal sequence, was expressed in bacillus subtilis using a b. amyloliquefaciens alpha-amylase secretion vector. to test the possibility of producing partially deleted dt molecules, which could be used for cell-directed toxin conjugates, a truncated form lacking 151 amino acids from the c-terminus of the dt was ... | 1989 | 2515098 |
| diphtheria: experience in a general hospital. | a review of patients with diphtheria seen in the paediatric unit, alor star general hospital, from january 1985-march 1987 is reported. their clinical presentation, diagnosis, treatment and outcome were analysed and discussed. clinical awareness regarding the diagnosis of diphtheria is emphasised. | 1989 | 2516602 |
| a fast spheroplast formation procedure in some 2,5-diketo-d-gluconate- and 2-keto-l-gulonate- producing bacteria. | calcium 2-keto-l-gulonate (ca-2-klg, a key intermediate in vitamin c synthesis) is produced from calcium 2,5-diketo d-gluconate (ca-2,5-dkg) by a variety of bacteria. a few bacterial species which efficiently convert glucose to ca-2,5-dkg have been isolated in our laboratory. our bacterial collection included species that possess the genes for production of ca-2-klg from ca-2,5-dkg; however, the yield of the former is poor. a procedure for the preparation of spheroplasts in ca-2,5-dkg- and ca-2- ... | 1989 | 2517394 |
| a report of the investigation and control measures instituted after the isolation of toxin-producing corynebacterium diphtheriae mitis from a child in leeds. | this report describes the control measures instituted and further investigations undertaken after the isolation of toxin-producing corynebacterium diphtheriae from a throat swab taken routinely from a nine-year-old boy, recently returned from pakistan, who had been admitted to hospital in leeds with hepatitis a infection. four of his siblings were subsequently shown to have identical corynebacterium diphtheriae on throat swabbing, except that in one child the organism did not produce toxin. all ... | 1989 | 2517490 |
| [isolation of corynebacterium diphtheriae from sperm fluid]. | the isolation of toxigenic corynebacterium diphtheriae from sperm is reported. the organism was identified through the investigation of fluorescence under the uv light, the presence of pirazinecarboxilamidase enzyme (pyz), in vitro and in vivo and virulence methods (single radial immunodiffusion, cell culture, guinea pig intradermic test). the strain was initially considered nontoxinogenic by single radial immunodiffusion, but its virulence was observed afterwards, when we applied the tests alre ... | 1989 | 2517567 |
| [suppuration caused by corynebacterium i2]. | | 1989 | 2524803 |
| acne necroticans (varioliformis) versus propionibacterium acnes folliculitis. | | 1989 | 2527876 |
| [the epidemic process of diphtheria infection in the rsfsr during the introduction of epidemiological surveillance]. | in this work the characterization of the epidemic process of diphtheria infection on the territory of the rsfsr under the conditions of epidemiological surveillance (1983-1986) is presented. in comparison with the period of 1979-1982, an increase in morbidity rate occurred, which accounts for more complete detection of patients with mild forms of diphtheria, including persons found to be carriers of toxigenic corynebacterium diphtheriae. beginning from 1983, the leveling out of seasonal morbidit ... | 1989 | 2528876 |
| [the use of dna fingerprint analysis for the differentiation of populations of toxigenic corynebacterium diphtheriae]. | 13 c. diphtheriae strains were used as a model to establish the conditions of making the fingerprint analysis of chromosomal dna. these strains, subdivided into 7 groups in accordance with the character of their restriction splitting, were mostly isolated from territorially close sources and belonged to the same phagotype. probably, c. diphtheriae dna has strain variations manifested by an unequal number and location of the sites of the recognition of specific endonucleases, which may be used fo ... | 1989 | 2530743 |
| microscopic changes of the urinary bladder in patients with primary tumors locally treated with corynebacterium parvum. | the study covered 34 patients with tumors of the urinary bladder treated surgically by transurethral resection and, as an adjuvant therapy, with corynebacterium parvum intravesically administered. microscopic observations were performed on smears stained with blue polychrome-tannin and the histologic study used preparata stained with hematoxylin-eosin and van gieson. after the second series of instillations, many biopsies from the same patient were taken. the investigation revealed the negativit ... | 1989 | 2533966 |
| potentiation by nonspecific immunostimulation of the efficacy of antibiotics in the treatment of experimental bacterial infections. | in the present investigation, mouse infection models with either intracellular or extracellular bacteria were designed in order to assess the effect of nonspecific immunostimulation with the c. granulosum-derived p40 immunomodulator on infection treatment, performed with doses of antibiotics achieving a low percentage of cures. the results obtained showed that nonspecific immunostimulation was able to significantly enhance antibiotic therapy efficacy. in addition, combined treatments of bacteria ... | 1989 | 2534887 |
| effect of sodium diethyldithiocarbamate, corynebacterium parvum and mycobacterium cell wall extract on in vitro blastogenic responses of bovine blood lymphocytes. | in vivo inoculation of three-month-old calves with sodium diethyldithiocarbamate (dtc), killed corynebacterium parvum or mycobacterium cell wall extract (mcwe) resulted in an enhancement of in vitro peripheral blood lymphocyte blastogenic responses to mitogens phytohemagglutinin (pha) and concanavalin a (con a) in the first three days after treatment. in a separate experiment, blood lymphocytes isolated from a healthy nontreated calf were incubated in vitro in presence of each of the same immuno ... | 1989 | 2536308 |
| a peptidyl dipeptidase-4 from pseudomonas maltophilia: purification and properties. | a peptidyl dipeptidase-4 (bacterial pdp-4) was purified to near homogeneity from a supernatant of pseudomonas maltophilia extracellular medium. bacterial pdp-4 is a single-polypeptide-chain enzyme, 82 kda, with an alkaline isoelectric point. peptides susceptible to hydrolysis by bacterial pdp-4 include angiotensin 1, bradykinin, enkephalins, atriopeptin 2, and smaller synthetic peptides. n-acylated tripeptides are hydrolyzed, but free tripeptides are not. a free carboxy terminus is required for ... | 1989 | 2539048 |
| in situ evaluation of the stimulatory state of hepatic macrophages based on their ability to produce superoxide anions in rats. | when a liver perfusion with nitro blue tetrazolium (nbt) and phorbol myristate acetate (pma) was performed in carbon tetrachloride (ccl4)-intoxicated rats, formazan deposition was remarkable in macrophages in the necrotic areas of the liver, its intensity varying with the extent of injury. the deposits almost disappeared after addition of cu(lys)2, a scavenger of intra- and extracellular superoxide, but were not affected by superoxide dismutase (sod), which acts extracellularly. the formazan con ... | 1989 | 2547045 |
| [bactericidal effect of daptomycin (ly 146032) compared with vancomycine and teicoplanin against gram-positive bacteria]. | daptomycin (ly 146032) is a semi-synthetic antibiotic whose structure consists of a cyclic peptide with a lipophilic chain. it inhibits synthesis of the bacterial wall differently than vancomycin and teicoplanin. the bactericidal activity of daptomycin was compared with that of vancomycin and of teicoplanin with regard to 54 gram positive strains of bacteria: (s. aureus meti s and meti r; s. epidermidis meti s and meti r; e. faecalis, e. faecium, and corynebacterium group jk). vancomycin and tei ... | 1989 | 2552378 |
| bronchoalveolar leukocyte response in experimental silicosis: modulation by a soluble aluminum compound. | the biological properties of quartz have been related to its surface reactivity. we have addressed the role of particle surface reactivity in mediating the biological activity of quartz in mixed dusts, by treating the quartz with aluminum lactate. intratracheal instillation of untreated quartz in rats caused a rapid, sustained alveolitis and bronchoalveolar leukocytes obtained from these animals had enhanced activity in degrading fibronectin, but reduced ability to mount a respiratory burst. qua ... | 1989 | 2552617 |
| prevalence of antibodies in goats in north-eastern brazil to selected viral and bacterial agents. | | 1989 | 2552630 |
| [the effect of 3',5'-cyclic adenylic acid (camp) on the growth of bacteria]. | the effect of intracellular camp level on the growth of bacteria was studied with e. coli as 1.797, corynebacterium pekinense as 1.299 and bacillus megaterium as 1.217. the experimental results show that the growth of e. coli as 1.797 was decreased with increasing camp level in cell. with glucose as the sole carbon source, intracellular camp is low, and growth of e. coli as 1.797 was inhibited by exogenous camp, but the analog metabolite of camp-5'-amp had no effect on growth. when e. coli as 1. ... | 1989 | 2552690 |
| ex vivo study of serum bactericidal titers and killing rates of daptomycin (ly146032) combined or not combined with amikacin compared with those of vancomycin. | twelve volunteers, in two groups of six, received daptomycin at a dose of 1 or 2 mg/kg. in addition, they received in a randomly allocated order amikacin (500 mg), daptomycin-amikacin, and vancomycin (500 mg). thirty-five clinical isolates, including staphylococcus aureus, s. epidermidis, corynebacterium sp. group jk, and enterococcus faecalis, were tested in vitro for the measure of the serum bactericidal titers and killing rates. the mean peak concentrations of daptomycin in serum 1 h after th ... | 1989 | 2556079 |
| phospholipase c in listeria. | the cooperative and antagonistic effect of extracellular bacterial proteins of streptococcus agalactiae, rhodococcus equi, corynebacterium ovis, and corynebacterium haemolyticum with proteins of listerial strains of various sources, species and serovars of the membrane of sheep erythrocytes was investigated. listeria monocytogenes and listeria ivanovii produce, beside listeriolysin, further proteins. their specific effect on the sheep erythrocyte membrane becomes apparent after the appearance of ... | 1989 | 2561039 |
| [the relationships between antitoxic titers determined in a group of young people before and after booster adt by the in vivo neutralization test. comparative nt-phar evaluations]. | the geometrical mean of antitoxic titers determined in a lot of immunized young men who received, 5-7 years before, a booster with diphtheria-tetanus bivaccine, was of 1.54 (x/divided by 10.54) i.u./ml. at one month after another booster, performed with tetanus toxoid (adsorbed), an increase of 34 times was recorded, the geometrical mean reaching the value of 52.67 (x/divided by 10.42 i.u./ml). the covering coefficients of the minimal protective antitoxin level have increased from 154 to 5267, i ... | 1989 | 2561337 |
| [flow cytometric analysis of the effect of activated macrophages on alveolar cell carcinoma of lung in vitro]. | evidences have shown that activated macrophages were cytotoxic to tumor cells but the mechanism of this killing effect is not well understood. flow cytometry was used to study the effect of activated macrophages on cell proliferation cycle of alveolar cell carcinoma of lung (a 549). among 74 c57bl/6j mice, 30 as experimental group were given corynebacterium parvum 0.5 ml (1 mg)/mouse intraperitoneally, and 44 as control group were given normal saline 0.5 ml/mouse instead. peritoneal macrophages ... | 1989 | 2561474 |
| autocytotoxic activity of lymphokine-activated killer cells: characterization of effector cells and susceptible targets. | the specificities and surface markers of murine autocytotoxic cells induced by in vitro culture with interleukin 2 (il2) were studied. culturing murine spleen cells with recombinant human il2 resulted in the generation of cytotoxic cells which killed syngeneic lymphoblasts and syngeneic activated macrophages (m phi). both lectins and protein antigens were capable of inducing lymphoblasts recognized by lymphokine-activated killer (lak) cells. b-lymphoblasts as well as t-lymphoblasts were sensitiv ... | 1989 | 2568770 |
| role of peptide-leukotrienes in liver injury in mice. | the role of peptide leukotrienes (p-lts), especially ltc4 and ltd4 in liver disease, was investigated in mice experimental liver injury models. the liver injury was induced by the injection of bacterial lipopolysaccharide (lps) into corynebacterium parvum pretreated mice. carbon tetrachloride (ccl4)-induced liver injury in mice was used as a standard model. in both injury models, extensive liver parenchymal cell damage was observed by the elevation of glutamate transaminase (got and gpt) activit ... | 1989 | 2575600 |
| diagnosis of corynebacterium pseudotuberculosis infections in sheep, using an enzyme-linked immunosorbent assay. | lambs infected with corynebacterium pseudotuberculosis (ovis) by injecting suspensions of live bacteria into the wool-free area of the axilla developed antibody against cell wall antigens and antitoxin, as measured by the enzyme-linked immunosorbent assay. the toxin was a better antigen for measuring infection than was the cell wall antigen. the enzyme-linked immunosorbent assay appeared to be as sensitive as the antihemolysin inhibition test for detecting antitoxin and was easier to perform. | 1985 | 2578756 |
| [interferon production and lymphoproliferation in whole blood of schizophrenia patients]. | using a whole blood assay the activity of the interferon system and mitogen-induced lymphoproliferation were tested in schizophrenic patients and normal individuals. leucocytes of the patients produced significantly less interferon than controls after stimulation with the alpha interferon inducers corynebacterium parvum (cp) or newcastle disease virus (ndv) and with the gamma interferon inducers pha and con a. differences in the lymphoproliferation test were also measured. significantly lower re ... | 1985 | 2581886 |
| [the significance of detecting corynebacterium equi in the conjunctiva of children in cairo]. | the probable correlations between certain corynebacterium are discussed. carpenter et al. (1976) describes a corynebacterium equi-pneumonia in a patient with hodgkin's disease; gardner et. al. (1976) also reports on a case of pneumonia. golub et. al. (1967) demonstrates a lung abscess resulting from corynebacterium equi. williams et. al. (1971) demonstrates a thoracic infection in an immunosuppressed patient. although, our findings have been demonstrated only in conjunctivitis of children, hithe ... | 1989 | 2583507 |
| regulation of macrophage physiology by l-arginine: role of the oxidative l-arginine deiminase pathway. | the l-arginine content of the extracellular fluid in sites of predominant macrophage infiltration is reduced below plasma levels due to the activity of macrophage-derived arginase. investigation of the effects of altered l-arginine availability on macrophage physiology reveals that culture of rat peritoneal macrophages in media containing l-arginine in the concentrations present in inflammatory lesions (less than 0.1 mm) enhances activation-associated functions. in contrast, culture in the highe ... | 1989 | 2584712 |
| [studies of the effects of bcg and corynebacterium parvum vaccines in experimental infection of mice with influenza virus. foot pad test, splenic indicator and histologic changes in the thymus gland, spleen and lymph nodes]. | evaluation of the influence of bcg and coparvax on reticulo-endothelial system in mouse was performed. mice were stimulated i.p. with bcg vaccine and coparvax vaccine. spleen index and histological changes in thymus, spleen and lymph nodes were evaluated after 14 days in mice vaccinated with bcg and after 7 days in mice vaccinated with coparvax. foot pad test was also performed by giving vaccine into three feet. tuberculin was injected into mouse foot pad on the day 7th and 14th and a lysate of ... | 1989 | 2586194 |
| effect of intracerebrally injected corynebacterium parvum on the development and growth of metastatic brain tumor in mice. | using kht tumor in a mouse metastatic tumor model, we examined the effect of intracerebral and/or intraperitoneal injections of corynebacterium parvum on the growth of metastatic brain tumor and the development of an inflammatory response in the central nervous system (cns). c. parvum given intraperitoneally had no effect on the development and growth of cns tumor, but did prolong the survival of mice by inhibiting the growth of systemic metastatic tumor, which was the cause of death in our tumo ... | 1989 | 2586725 |
| [the inhibitory effect on passive cutaneous anaphylaxis in guinea pigs of oral administration of corynebacterium (rhodococcus) equi]. | we studied the inhibitory effect on the pca reaction of bsa-rabbit anti-bsa (bsa-anti-bsa) system in cp-sensitized and cp-unsensitized guinea pigs by the oral administration of c. equi. strain ko-85 (ko-85) cells. the results were as follows: 1) guinea pigs were sensitized by intraperitoneal injection with the cp. nine days later, the animals were administered with an oral dose of 80 mg (w.w.) of ko-85 cells. the animals were submitted daily to pca reaction tests using bsa and rabbit anti-bsa se ... | 1989 | 2589991 |
| observations on bovine pyelonephritis. | fifty-one cases of bovine pyelonephritis were investigated. the annual prevalence rate for 2089 cows was 1.6% in 1987 with a mean interval from calving to onset of cases of 82.9 days. multipara were at higher risk. loss from pyelonephritis was 33.3% of all affected cows, and relapse occurred in 9.4% of apparently recovered animals. serum creatinine and urea concentrations were of high prognostic values. odds ratios being culled for cows with levels of creatinine above 1.5 mg/dl (132.6 mumol/l) a ... | 1989 | 2590828 |
| separation of homologues of methyl ester and 3-o-acetyl methyl ester derivatives of the corynomycolic acid fraction from corynebacterium pseudotuberculosis. | | 1989 | 2592501 |
| comparison of the nasal bacterial floras in two groups of healthy subjects and in patients with acute maxillary sinusitis. | the nasal bacterial flora was studied in 183 healthy men entering military service (entry group), 103 healthy recruits in service (service group), and 185 recruits with acute maxillary sinusitis. the 267 nasal and ipsilateral sinus aspirate findings in the same patients with acute maxillary sinusitis were compared pairwise. in the entry group presumed sinus pathogens were only rarely isolated from the nasal cavities: haemophilus influenzae in 4%, streptococcus pneumoniae in 1%, branhamella catar ... | 1989 | 2592539 |
| corynebacterium xerosis as a cause of vertebral osteomyelitis. | we report a patient who developed corynebacterium xerosis vertebral osteomyelitis 6 months following a decompressive laminectomy. prolonged parenteral and subsequent oral therapy for 11 months resulted in apparent cure. this is the first reported case of vertebral osteomyelitis caused by c. xerosis. | 1989 | 2592549 |
| infective endocarditis in black south african children: report of 10 cases with some unusual features. | ten black south african children with infective endocarditis seen over a 2-year period are reported. in five cases, including two neonates, the infection was nosocomial and in five cases it occurred in children with previously normal hearts. of the bacteria isolated from nine cases, five were staphylococcus aureus (all from nosocomial cases), one was haemophilus influenzae and three were corynebacteria. the unusual aspects of this series are discussed, with an emphasis on preventing nosocomial c ... | 1989 | 2594454 |
| septicaemia in a granulocytopenic patient caused by corynebacterium striatum. | a 64 year old woman with metastatic endometrial carcinoma was admitted to the hospital after three grand mal seizures. blood cultures yielded corynebacterium striatum. the patient responded to parenteral ampicillin therapy. this is believed to be the first case of sepsis caused by this organism. | 1989 | 2594603 |
| [caseous lymphadenitis in moorland sheep in the netherlands]. | caseous lymphadenitis was detected in two flocks of moorland sheep originating from western germany. retrospective serological examination showed that the nucleus flock was infected as early as the time of importation in 1984. | 1989 | 2595714 |
| second-look laparotomy in the patient with minimal residual stage iii ovarian cancer (a gynecologic oncology group study). | one hundred eighty-six patients with minimal residual stage iii ovarian cancer (tumor mass less than or equal to 3 cm) were treated in a prospective randomized protocol (melphalan with or without corynebacterium parvum). as per protocol 84 patients were eligible and underwent a second-look laparotomy with 41 (49%) having negative findings for persistent malignancy. factors which affected survival after second look were presence or absence of macroscopic disease, age, and grade. depending upon th ... | 1989 | 2599475 |
| studies on route of immunization with a mixture of killed parasites and adjuvants against babesia rodhaini infection in mice. | a series of experiments were undertaken to determine the most effective route of immunization with a mixture of killed babesia rodhaini antigen (s antigen) and formalin-fixed corynebacterium parvum (propionibacterium acnes) bacterin (cpb) against challenge infection with b. rodhaini 3 weeks later. the mice pretreated with s antigen and cpb mixture intraperitoneally, but not intramuscularly, were significantly resistant to intraperitoneal (ip) or intravenous (iv) challenge with 10(6) organisms. t ... | 1989 | 2601229 |
| effect of corynebacterium parvum bacterin on artificial immunity against babesia rodhaini infection in mice. | | 1989 | 2601237 |
| systemic administration of il-2 induces lymphokine-activated killer (lak) cells capable of killing macrophages in various tissues. | murine lymphokine-activated killer (lak) cells induced by systemic high-dose recombinant human interleukin 2 (il-2) administration lysed fresh syngeneic peritoneal macrophages (m phi). lak cells lysed resident peritoneal m phi and m phi activated in vivo with thioglycollate (tg), corynebacterium parvum (c. parvum), or bacillus calmette-guérin (bcg). the induction of anti-m phi cytolytic activity was seen in the spleen, liver, lung, lymph nodes and peritoneal cavity, but was not observed in the t ... | 1989 | 2606510 |
| differential expression of membrane sialoglycoproteins in exudate and resident mouse peritoneal macrophages. | wheat germ agglutinin (wga) has been used to define biochemical differences between exudate and resident macrophages in the peritoneal cavity of mice. western blotting with 125i-wga identifies a restricted set of glycoproteins in elicited peritoneal macrophages (m phi) (recruited with thioglycollate or periodate) and fully activated m phi (recruited with live bacille calmette-guérin, or heat-killed corynebacterium parvum); the major species migrates with mr 89-115 kda in 10% acrylamide gels. the ... | 1989 | 2606943 |
| [group jk (corynebacterium jeikeium sp. nov.) isolated from japanese skin flora]. | in many parts of the world, the emergence of multiple antibiotic resistant group jk has been recognized as an important cause of nosocomial infection in immunosuppressed and neutropenic patients. group jk infections have not been reported from japan. we studied 20 japanese who had recently come to the united states and recovered group jk from 7 of 20. these results suggest that group jk may be prevalent in japanese. previous failure to identify these organisms may reflect their poor growth on ag ... | 1989 | 2607672 |
| prolonged adjuvant chemotherapy in breast cancer. the scandinavian adjuvant chemotherapy study 2. | eleven hundred and sixteen primary female breast cancer patients received one short perioperative chemotherapy course. the node negative patients were randomised between immunotherapy (corynebacterium parvum s.c. around the scar 2 weeks after mastectomy), or no further adjuvant therapy. a moderate, but significant delaying effect was observed, without side effects. the node positive patients were randomised to four groups: 1) the same immunotherapy as in the node negative patients, 2) cmf for 1 ... | 1989 | 2611040 |
| [the role of ca2+ ions in a phage infection of corynebacterium glutamicum]. | it was shown that neither uncouplers of oxidative phosphorylation, nor lack of ca2+ ions affected the normal mc-2 phage absorption on corynebacterium glutamicum cells, while the phage development was repressed under these conditions. simultaneous measurement of ca2+, k+ and h+ ion flows, as well as measurement of membrane potential showed that the addition of the phage into the experimental medium led to significant depolarization of the membrane from -160 mv to -100 mv due to the penetration of ... | 1989 | 2611280 |
| [cloning and structural analysis of the operon of ribosomal rna of corynebacterium glutamicum]. | | 1989 | 2612356 |
| high efficiency electroporation of intact corynebacterium glutamicum cells. | high-frequency electroporation of whole corynebacterium glutamicum cells without enzymatic pretreatment was achieved. under optimized conditions concerning growth stage, washing of cells, cell concentration and pulse parameter transformation efficiencies of far more than 10(7) transformants per microgram pwst4b plasmid dna were reached. using electroporation, linearised and subsequently religated plasmid as well as chimeric ligase reaction products were directly introduced into c. glutamicum wit ... | 1989 | 2612892 |
| molecular cloning, nucleotide sequence and fine-structural analysis of the corynebacterium glutamicum fda gene: structural comparison of c. glutamicum fructose-1,6-biphosphate aldolase to class i and class ii aldolases. | the corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate (fbp) aldolase has been isolated by complementation of an escherichia coli mutant. the nucleotide sequence of a 3371 bp chromosomal fragment containing the c. glutamicum fda gene was determined. the n-terminal amino acid sequence of c. glutamicum fbp aldolase identified the correct initiation site for the fda gene, and a molecular weight of 37,092 was predicted for the fda polypeptide. s1 nuclease mapping identified the tr ... | 1989 | 2615658 |
| [the aerobic air microflora in airplanes on various international routes]. | aerobic microflora (bacteria, fungi), in the cock pits of the tarom company (boeing 707 and il 62 m) airships flying on various international routes and airports was studied during november 1988-january 1989. 157-8,800 bacteria and 78-1,336 fungi per m3 air were recorded. except for staphylococcus aureus (hemolytic and non hemolytic) the greatest part of the isolated microorganisms was nonpathogenic for man: bacillus, corynebacterium, neisseria, staphylococcus epidermidis, sarcina, aspergillus, ... | 1989 | 2616999 |
| corynebacterium pseudotuberculosis infection in camels (camelus dromedarius) in saudi arabia. | | 1989 | 2617677 |
| characterization of a neuraminidase from corynebacterium aquaticum responsible for th polyagglutination. | th polyagglutinability is characterized by the agglutination of the red blood cells (rbc) by arachis hypogaea, medicago disciformis, vicia cretica but, in contrast to the t phenomenon, not by glycine max (glycine soja). because th transformation of rbc has been obtained in vitro, the mechanism of th polyagglutinability expression has been studied and reproduced experimentally. an enzyme with neuraminidase specificity has been isolated from the culture supernatant of corynebacterium aquaticum, an ... | 1989 | 2617954 |
| a new bacteriophage of corynebacterium glutamicum isolated from swine waste. | a bacteriophage for corynebacterium glutamicum strain lp-6 was isolated from swine waste. it belongs to the siphoviridae family or bradley morphologic group b, has a narrow host range, and is sensitive to chloroform and resistant to carbon tetrachloride. the phage is unstable (96% inactivation) in swine waste stored for 4 months at 22 c. the dna has a molecular weight of approximately 20 md, cohesive ends, and numerous restriction endonuclease sites. the phage differs from other known c glutamic ... | 1989 | 2619124 |
| [normal microflora of the pharyngeal mucosa]. | aerobic microflora of the throat mucosa was studied in 518 healthy persons aged 1 to 50 years. on the basis of the study results, criteria for estimating microbiocenoses of the upper respiratory tracts were defined. it was shown that the throat symbiotic flora included three groups of microorganisms playing different roles in the development of microbiocenosis. the indigenous group consisted of representatives of streptococcus and neisseria and was characterized by permanent (90-100 per cent) an ... | 1989 | 2619405 |
| increasing incidence of corynebacterium group d2 strains resistant to norfloxacin and ciprofloxacin. | | 1989 | 2620675 |
| nonspecific stimulation of host defense by corynebacterium kutscheri. i. antitumor effect. | the effect of local injection of formalin-killed corynebacterium kutscheri (fk.ck) on mouse survival after the intraperitoneal inoculation of ehrlich ascites carcinoma in outbred ddy mice or p388 leukemia cells in inbred cdf1 mice was investigated. treatment of mice in the dose range of greater than 10(6) organisms per mouse conferred the substantial protection on both mice. the initial phase of antitumor effect consisted of the marked increase in the number of peritoneal exudate cells and the e ... | 1989 | 2622460 |
| immunological identification and distribution of dissimilatory heme cd1 and nonheme copper nitrite reductases in denitrifying bacteria. | polyclonal antibodies were used to identify heme or copper nitrite reductases in the following groups: 23 taxonomically diverse denitrifiers from culture collections, 100 numerically dominant denitrifiers from geographically diverse environments, and 51 denitrifiers from a culture collection not selected for denitrification. antisera were raised against heme nitrite reductases from pseudomonas aeruginosa and pseudomonas stutzeri and against copper nitrite reductase from achromobacter cycloclaste ... | 1989 | 2624465 |
| microbial investigations in keratitis at the sydney eye hospital. | we investigated 107 cases of presumed microbial keratitis amongst patients presenting to the sydney eye hospital between october 1986 and august 1988 to determine the frequency of infection, the common causative organisms and those factors that predispose to corneal infection. we found that 95% of culture-proven cases were caused by bacteria and that staphylococcus epidermidis and corynebacterium sp. accounted for 42.5% of these cases. all bacteria (except staphylococcus epidermidis and mycobact ... | 1989 | 2624733 |
| prevention of bovine mastitis by a postmilking teat disinfectant containing chlorous acid and chlorine dioxide in a soluble polymer gel. | a natural exposure study was conducted in a herd of 150 lactating dairy cows for 18 mo to determine the effectiveness of chlorous acid and chlorine dioxide in a soluble polymer gel as a postmilking teat disinfectant for the prevention of bovine mastitis. right quarters of cows were dipped in the experimental teat dip after milking machine removal. left quarters were not dipped and served as within-cow negative controls. the experimental teat dip reduced staphylococcus aureus infections 67.4%, st ... | 1989 | 2625499 |
| corynebacterium pseudotuberculosis infection in goats. viii. the effect of vaccination against experimental infection. | the effect of an inactivated vaccine against c. pseudotuberculosis infection was tested on castrated male kids from a herd free from caseous lymphadenitis. the animals were divided into 3 groups with 8 animals in each. group 1 was immunized with crude filtrated c. pseudotuberculosis toxoid and whole killed organisms, while group 2 in addition was given levamisole. the kids were vaccinated twice at an interval of 4 weeks. group 3 consisted of unvaccinated animals. all groups were challenged subcu ... | 1989 | 2629504 |
| corynebacterium pseudotuberculosis infection in goats. ix. the effect of vaccination against natural infection. | a vaccination trial was carried out in 10 infected herds. the trial included 247 female kids, the number of animals in each herd varying from 15 to 38. about half of the animals in each herd were vaccinated twice at 3 to 4 week intervals, the first vaccination being carried out before the age of 4 months. a combination of a crude filtrate of c. pseudotuberculosis toxoid with whole organisms, was used. overall, the prevalence of animals with superficial swellings was higher in the unvaccinated th ... | 1989 | 2629505 |
| [endocarditis due to corynebacterium xerosis]. | corynebacterium sp. are found as normal flora in skin and mucosal sites. they have been isolated in empyemas, brain abscesses, blood cultures and ventricular shunts. about 9-10% of early-onset and 4-5% late-onset prosthetic valve endocarditis are due to different species of the so-called "diphteroids". a 30 year-old white female was admitted after 30 days with fever of undetermined origin. a mitral prosthesis had been fitted in 1977. on physical examination a protomesosystolic mitral murmur, pet ... | 1989 | 2630875 |
| sheep major histocompatibility (ola) complex: apparent involvement in a flock endemic infection by corynebacterium pseudotuberculosis. | in a préalpe flock with endemic infection by corynebacterium pseudotuberculosis, some sires seem to have transmitted some resistance traits not yet genetically defined. in sire progenies including offspring with and without abscess, abscesses occurred independently of the transmitted sire's ola haplotype. however, in all considered offspring, some ola antigens were positively or negatively associated with the delayed occurrence of abscess. the three antigens ola-a4, a10, b6 were positively assoc ... | 1989 | 2631799 |
| influence of corynebacterium bovis on constituents of milk and dynamics of mastitis. | | 1989 | 2635007 |
| [tonsillitis]. | the tonsils, paired structures which protect the pharynx, are frequently the target of infections, most often in pediatric patients. etiology of tonsillitis may be viral (adenovirus) as well as bacterial (corynebacterium diphtheria, among others). clinically, the disease may manifest congestive disturbance and exudative membranes, it may have local or systemic complications, and its treatment is symptomatic. | 1989 | 2639343 |
| the effect of corynebacterium parvum in influenza infected mice. | this paper is the continuation of earlier studies on the effect of the killed suspension of corynebacterium parvum in influenza virus infected mice. our investigation showed the normalized effect of these drugs on disturbed function of cell mediated immunity during experimental influenza infection especially in phagocytic and bactericidal activity of granulocytes. the present experiments concern the explanation of these infection mechanisms. intraperitoneal injection of corynebacterium parvum st ... | 1989 | 2639639 |
| [preliminary studies of the effect of steroid hormones on skin bacteria in vitro]. | it results from studies on skin hormones that some of steroid hormones reach high concentrations on skin surface mainly during a course of acne vulgaris. our studies indicate that the activity of hormones on skin bacteria can be multiform. nineteen hormones of analytical grade of purity were tested. they were derived from different firms of synthesized in the department of endocrinology of the pharmacology institute of polish academy of sciences in cracow. bacterial strains tested in this study ... | 1989 | 2640684 |
| restoration of prostaglandin e2-producing splenic macrophages in 89sr-treated mice with bone marrow from corynebacterium parvum primed donors. | administration of corynebacterium parvum (cp), 56 mg/kg ip to cba/j mice effected the induction of prostaglandin e2 (pge2) producing macrophages (m phi) in the bone marrow and the spleen. maximal release of pge2 from m phi cultured in vitro with calcium ionophore a23187 for 2 h was reached by marrow m phi removed on 5 days after cp (450 ng/mg cell protein), and by splenic m phi 9 days after cp (400 ng/mg). neither m phi population, however, yielded more than 6.0 ng/mg leukotriene c4. to assess o ... | 1989 | 2641504 |
| characterization of a wide host range plasmid panv-6 from citrobacter diversus. | panv-6 is a 5.85 kb, streptomycin resistant, high copy number plasmid isolated from a multi drug resistant clinical isolate of c. diversus by transformation in escherichia coli c-600. the plasmid was very stable, noncurable and could not be amplified with chloramphenicol. it was non-conjugative among enterobacteriaceae hosts. plasmid was transferred by transformation to several gram negative and gram positive hosts. these included esch. coli, serratia marcescens, salmonella typhimurium, klebsiel ... | 1989 | 2644170 |
| mycotic omasitis and rumenitis as sequelae to sepsis in dairy cattle: six cases (1979-1986). | medical records of 6 cows with mycotic rumenitis or omasitis, but no history of grain engorgement, were reviewed. a diagnosis was made at necropsy in all cows, although the condition was suspected before euthanasia in 3 cows. common clinical findings included inappetence, rumen stasis, fluid rumen contents, and scant, pasty, or loose feces. postmortem findings included severe ulcerative rumenitis or omasitis, with thrombosis of blood vessels in the submucosa, and branching, septate fungal hyphae ... | 1989 | 2646269 |
| nephrolithiasis resulting in intermittent ureteral obstruction in a cow. | bilateral nephrolithiasis with intermittent ureterolithiasis was diagnosed in a 7-year old holstein cow. two episodes of ureterolithiasis resulted in severe azotemia which resolved after spontaneous movement of the stone. a third episode of obstruction one year after the initial episode resulted in rupture of one kidney, necessitating euthanasia. the histopathological examination of the kidney was diagnostic for chronic pyelonephritis. corynebacterium sp. was cultured from a nephrolith. in this ... | 1989 | 2647406 |
| [infective endocarditis in children. apropos of 13 cases]. | from 1978 through 1987, thirteen pediatric patients aged 14/12 years to 16/12 years were hospitalized for infective endocarditis (ie). ten cases presented as acute septicemia with modification or development of a murmur and/or heart failure. three patients had subacute endocarditis. prior to the endocarditis, ten patients had recognized heart disease, whereas three had no known cardiac abnormality. the organism was recovered in seven cases, from blood cultures in six cases (3 staphylococcus aure ... | 1989 | 2648936 |
| pyelonephritis in cows: 15 cases (1982-1986). | medical records of 15 adult cows with a final diagnosis of pyelonephritis were evaluated retrospectively. only 3 cows had obvious clinical signs that indicated urinary tract disease. physical examination, including rectal and vaginal examinations, and urine chemical reagent strip screening detected abnormalities in all 15 cows and allowed subsequent confirmation by culture results of urine samples obtained using a catheter. bacteriologic cultures of urine revealed corynebacterium renale infectio ... | 1989 | 2649473 |
| [penile inflammation in ganders]. | since 1987 penis-inflammation and -prolapse were observed in north-german breeder-geese. up to 28% of the ganders showed local symptoms. in the females no clinical signs of cloaca-inflammation were seen. the general condition of the birds was good and there was no increased mortality. egg production and fertility were not influenced. bacteriological examinations of the altered penis-tissues revealed in different frequency microorganisms belonging to the genera mycoplasma, candida, bacteroides, c ... | 1989 | 2651081 |
| spontaneous cytotoxicity of macrophages against pancreatic islet cells. | activated peritoneal macrophages were found to lyse syngeneic [3h]leucine-labeled pancreatic islet cells or rat insulinoma cells after 15 h of coculture at 37 degrees c. lysis was verified by electron microscopic analysis. islet cell lysis was dependent on the t:e ratio and was comparable with p815 and l929 tumor cells used as targets. the cytotoxic activity was localized in the adherent fraction of corynebacterium parvum activated peritoneal cells and was destroyed by incubation of cells with m ... | 1989 | 2654293 |
| [neutrophil defense against bovine uterine infections]. | the killing ability of bovine peripheral blood neutrophils did not vary during the oestrous cycle. administration of sex steroids to ovariectomised cows reduced the intrinsic killing ability of neutrophils but enhanced the opsonising ability of serum. exudate from experimental uterine infection with corynebacterium pyogenes and fusobacterium necrophorum impaired neutrophil function, probably as a result of the action of bacterial leucotoxins. | 1989 | 2655163 |
| [cloning and study of corynebacterium glutamicum genes complementing ilva and thra2 mutations in escherichia coli]. | molecular cloning and expression of corynebacterium glutamicum genes complementing escherichia coli mutations thra2 and ilva was performed. it was demonstrated that the thra2 gene of c. glutamicum is located close to thrb on ecori dna fragment 4.1 kb long. the fragment was cloned in puc18 vector. the thra2 gene is expressed in the recombinant plasmid pobt3 under control of the vector puc18 plac promoter. in e. coli minicells, the genes thra2 and thrb determined synthesis of proteins of mr 43kd a ... | 1989 | 2659439 |
| intravascular coagulation in the development of massive hepatic necrosis induced by corynebacterium parvum and endotoxin in rats. | when escherichia coli endotoxin was intravenously injected into rats given killed corynebacterium parvum 6 days previously, fibrin deposition and endothelial cell injury occurred in hepatic sinusoids at 1.5 h and were intensified thereafter. serum alanine aminotransferase values were increased along with prothrombin time and decreased plasma levels of antithrombin iii and coagulation factor viii:c at 5 h. antithrombin iii concentrate (plus heparin) or superoxide dismutase infused concurrently wi ... | 1989 | 2660248 |
| enhancement of the infectivity of fusobacterium necrophorum by other bacteria. | necrobacillosis is caused by fusobacterium necrophorum (fn), but other organisms are often present in the lesions. their possible role was studied in experiments made with a virulent fn strain which, by itself, produced fatal necrobacillosis in mice provided that large doses (greater than 10(6) organisms, subcutaneously) were given. mice were inoculated subcutaneously with fn suspended in sub-lethal doses (0.1 ml) of undiluted or diluted broth cultures of other bacteria. undiluted culture of a s ... | 1989 | 2661253 |
| cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of corynebacterium glutamicum atcc13032. | as a first step in determining the importance of the anaplerotic function of phosphoenolpyruvate carboxylase (pepc) in amino acid biosynthesis, the ppc gene coding for pepc of corynebacterium glutamicum atcc13032 has been cloned by complementation of an escherichia coli ppc mutant strain. pepc activity encoded by the cloned gene is not affected by acetyl-coa under conditions where the e. coli enzyme is strongly activated, whereas acetyl-coa is able to relieve inhibition by l-aspartate used singl ... | 1989 | 2666264 |
| laboratory survey of fluoroquinolone activity. | fluoroquinolones are active against a wide variety of bacteria. the antibacterial spectra of fluoroquinolones encompass staphylococci, bacillus species, and corynebacterium species implicated in infections of the immunocompromised host; enterobacteriaceae; most intestinal pathogens; and many gram-negative organisms commonly causing nosocomial infections. haemophilus influenzae, haemophilus ducreyi, neisseria gonorrhoeae, neisseria meningitidis, and branhamella catarrhalis are highly susceptible ... | 1989 | 2672262 |
| serological investigation of corynebacterium pseudotuberculosis infection in sheep--correlation between the hemolysis inhibition test and the elisa test. | | 1989 | 2675582 |
| [treatment of peritonitis under continuous ambulatory peritoneal dialysis using intraperitoneal ceftazidime]. | management of peritonitis related to chronic ambulatory peritoneal ceftazidime. in 15 patients under capd, 22 cases of bacterial peritonitis were observed with clinical manifestation in 14. the mean cell count in peritoneal dialysis fluid was 3 580/mm3 with 3 040/mm3 polymorphonuclear leukocytes. causative pathogens were: staphylococcus in 11 cases, streptococcus in 3, sarcines in 2, corynebacterium in 2, micrococcus varians in 1, gram negative in 3. first choice treatment was a intraperitoneal ... | 1989 | 2677930 |
| cause, occurrence, and clinical signs of mastitis and anorexia in cows in a wisconsin study. | microbiologic culture revealed the following cause of mastitis and anorexia in 145 cows in wisconsin to be escherichia coli, 66 cows; klebsiella spp, 3; corynebacterium pyogenes, 27; streptococci, 21; staphylococci, 20; yeasts, 1; and no bacterial growth, 7. mastitis was detected with approximately equal frequency throughout the year. escherichia coli was isolated throughout the year, but was more common and was the predominant organism during the summer. corynebacterium pyogenes was isolated mo ... | 1989 | 2681108 |
| structural and functional analysis of a bacterial cellulase by proteolysis. | cena is an endo-beta 1,4-glucanase from the cellulolytic bacterium cellulomonas fimi. it is a bifunctional enzyme comprising an amino-terminal cellulose-binding domain and a carboxyl-terminal catalytic domain joined by a short sequence of prolyl and threonyl residues (the pro-thr box). additional structural and functional information was revealed by a detailed analysis of the products generated by proteolytic cleavage of a nonglycosylated form of cena. an extracellular c. fimi protease attacked ... | 1989 | 2681184 |
| intramammary infections and risk factors for clinical mastitis in herds with low somatic cell counts in bulk milk. | ten herds with low somatic cell counts in bulk milk had an incidence of clinical mastitis of only 2.2 per 100 cows whereas 10 other herds with similarly low cell counts had an incidence of 53.6 per 100 cows. the major pathogens in the herds with a high incidence were escherichia coli, streptococcus uberis, staphylococcus aureus and the coagulase-negative staphylococci. the percentage of uninfected quarters in the herds with a high incidence of clinical mastitis was 21.4 per cent compared with 12 ... | 1989 | 2683341 |
| glutaredoxin from rabbit bone marrow. purification, characterization, and amino acid sequence determined by tandem mass spectrometry. | a glutaredoxin was purified from rabbit bone marrow, and its amino acid sequence was determined by high performance tandem mass spectrometry. the sequences of peptides generated by digestion with trypsin alone or in combination with thermolysin were determined from their collision-induced dissociation (cid) mass spectra. alignment of these sequences and additional sequence information were obtained from the collision-induced dissociation mass spectra of peptides obtained from digestion of the in ... | 1989 | 2684977 |