| [the immunological effectiveness of small doses of diphtheria and tetanus anatoxins in the revaccination of children with allergy-altered reactivity and adults]. | the possibility of decreasing the content of antigens in adsorbed diphtheria-tetanus toxoid, used for the booster immunization of children with allergically altered responsiveness and adults, is shown. diphtheria toxoid in doses of 1-2.5 lf and tetanus toxoid in doses of 1-2.5 binding units possessed sufficiently high immunological effectiveness in combination with low reactogenicity and good tolerance. in 6-12 months after booster immunization with decreased doses of toxoids 100% of children wi ... | 1990 | 2117319 |
| effect of different concentrations of diphtheria toxoid & aluminum phosphate on potency of diphtheria component in dpt vaccines. | an effort was made to determine the optimum concentration of diphtheria toxoid in combination with aluminum phosphate gel in dpt vaccines which may give a safe, potent and economical preparation. the effect of four different concentrations of aluminum phosphate and three different antigenic concentrations of diphtheria toxoid on potency of diphtheria component in dpt vaccine was assessed. a gradual increase in potency was seen with increase in toxoid concentration and a gradual decrease in poten ... | 1990 | 2118880 |
| mechanisms of macrophage-mediated tumor cell killing: a comparative analysis of the roles of reactive nitrogen intermediates and tumor necrosis factor. | the roles of tumor necrosis factor (tnf alpha) and reactive nitrogen intermediates (rni) as effectors of macrophage-mediated tumor cell killing were investigated in a variety of tumor cell lines. three tnf alpha-sensitive tumor targets were also susceptible to resting bone-marrow-derived mononuclear phagocytes (bmmp). this macrophage lytic activity was markedly diminished or even abolished by anti-tnf alpha, indicating that tnf alpha is the major effector of macrophage-mediated killing of these ... | 1990 | 2120138 |
| resistance to a non-immunogenic tumor, induced by corynebacterium parvum or listeria monocytogenes, is abrogated by anti-interferon gamma. | the complex processes that determine the outcome of the interaction of tumor and host were explored in the operationally simple and reproducible rat d-12 ascites tumor model. animals exhibit weak spontaneous resistance against this tumor that is not augmented by repeated inoculation, by various routes, of irradiated syngeneic d-12 tumor cells, but considerably enhanced after local administration of heat-killed corynebacterium parvum (cp) or listeria monocytogenes (lm) organisms. inoculation of c ... | 1990 | 2120139 |
| the effect of selenium supplementation on antibody response to bacterial antigens in merino sheep with a low selenium status. | | 1990 | 2121126 |
| [the cellular immune response in different forms of diphtheria in adults]. | in 109 adult diphtheria patients with different forms of the disease (toxic, subtoxic, localized) and carriers, the latter including 6 persons having had the localized form of diphtheria, 12 different subpopulations of t and b lymphocytes and neutrophils were studied by the methods of rosette formation with sheep, mouse and bovine red blood cells. the study showed that, starting from week 2 of the disease, an increase in the content of the subpopulations under study was registered in all forms o ... | 1990 | 2122622 |
| lysine uptake and exchange in corynebacterium glutamicum. | resting cells of corynebacterium glutamicum (atcc 13032) accumulate [14c]lysine by a transport system with a relatively high affinity (10 microms) and a low maximum velocity (0.15 nmol/min per mg [dry weight]). uptake of lysine was not inhibited by uncouplers or by ionophores affecting the ion gradients and the energetic state of the cell. analysis of intracellular amino acid concentrations during the transport reaction as well as kinetic studies revealed that the observed uptake of lysine in fa ... | 1990 | 2123868 |
| sandwich-dot immunobinding assay (sandwich-dia), a new immunological method for the detection of diphtheria toxin. | in this paper we describe a new assay for diphtheria toxin in bacterial cultures, based on a sandwich-dot immunobinding method. this method uses horse polyclonal diphtheria antitoxin as the coating antibody and mouse monoclonal diphtheria antitoxin as the detecting antibody. the sensitivity of this new method is within a range of 5-10 ng toxin per ml in corynebacterium diphtheriae cultures. we did not observe any false-positive reactions. as a solid phase we used nitrocellulose disks which bind ... | 1990 | 2124491 |
| cellular fatty acid composition of oerskovia species, cdc coryneform groups a-3, a-4, a-5, corynebacterium aquaticum, listeria denitrificans and brevibacterium acetylicum. | twenty four strains representing eight species of gram positive yellow-pigmented rods (oerskovia turbata, oerskovia xanthineolytica, cdc coryneform groups a-3, a-4, a-5, listeria denitrificans, corynebacterium aquaticum and brevibacterium acetylicum) were divided into two major groups based on the relative amounts of 12 methyltetradecanoate (15:0a) obtained by capillary gas liquid chromatography. o. turbata, o. xanthineolytica, cdc groups a-3 and a-4, l. denitrificans and c. aquaticum were place ... | 1990 | 2124793 |
| intergeneric protoplast fusion between xylanase producing bacillus subtilis lyt and corynebacterium acetoacidophilum atcc 21476. | hybrids between a strain of bacillus subtilis isolated in our laboratory and having the ability to degrade xylan and other complex polysaccharides and corynebacterium acetoacidophilum, a lysine producer, were prepared by protoplast fusion. based on distinctive parental biochemical characteristics the fusants were grouped into 9 categories, viz. bc1 through bc9. three of the hybrids, bc5, bc7a and bc7b, were tested for their ability to produce xylanase and lysine. both bc7a and bc7b produced xyla ... | 1990 | 2125571 |
| in vitro activity of fleroxacin against aerobic gram-positive bacteria including corynebacterium jeikeium. | fleroxacin (ro 23-6240, am-833), a new fluoro-4-quinolone, was tested in vitro against 273 gram-positive clinical isolates. norfloxacin, a quinolone mostly used in urinary tract infections, was included as a standard. overall, in vitro activities of fleroxacin and norfloxacin were superposable, but interestingly, fleroxacin was two- to fourfold more active against staphylococcus aureus, and 1 mg/l of fleroxacin inhibited all 5 multiply resistant corynebacterium jeikeium tested. while both quinol ... | 1990 | 2127242 |
| in vitro activity of sparfloxacin (ci-978; at-4140) against clinical isolates from cancer patients. | the in vitro activity of sparfloxacin, a new quinolone, was compared with those of ciprofloxacin and fleroxacin against gram-positive and gram-negative bacteria, greater than 90% of which were isolated from blood culture specimens of cancer patients. sparfloxacin was extremely active against acinetobacter species, aeromonas hydrophila, citrobacter diversus, enterobacter species, escherichia coli, klebsiella species, proteus vulgaris, and serratia marcescens (inhibiting greater than 90% of these ... | 1990 | 2127348 |
| the molecular structure of the corynebacterium glutamicum threonine synthase gene. | the minimal region encoding the corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the c. glutamicum thrc allele to a 1.6 kb region of the recombinant plasmid pfs80. the nucleotide sequence of this and flanking dna was determined. the transcription and translation start points were identified by s1 mapping analysis and amino-terminal protein sequencing, respectively. the thrc gene encodes a 54481-dalton polypeptid ... | 1990 | 2127631 |
| [the immunomodulating properties of a diphtheria bacterial vaccine in in vitro experiments]. | in this investigation lymphocytes sensitized with corynebacterium diphtheriae antigens obtained from carriers and convalescents were used. the new diphtheria bacterial vaccine codivac, in contrast to other comparable preparations (diphtheria toxoid, staphylococcal toxoid, staphylococcal vaccine, levamisole), was found to produce a more direct effect by modulating the levels of t-lymphocytes, depending on their initial levels in the patient. codivac, together with other preparations, can be used ... | 1990 | 2129148 |
| nucleotide sequence of the dapa gene from corynebacterium glutamicum. | | 1990 | 2129555 |
| [changes in the aortic arch of calves with bacterial infections]. | the autopsy and histopathological changes in the wall of aortic arch were presented in calves died of pulmonary and intestinal disorders. the bacteriological examinations of the internal organs demonstrated: e. coli, pseudomonas aeruginosa corynebacterium et al. gelatinous edematous++ and hemorrhagic inflammatory centers of aortic arch adventitia were distinctly visible during thorax examination. in aortic arch the diagonal fissures of endothelium and deeper layers of the wall were found. in mic ... | 1990 | 2132656 |
| [a microbiologic study on anaerobic bacteremia after dental extraction]. | it has been known for many years that tooth extraction can cause bacterial dissemination into blood stream, that is, bacteremia. besides endocarditis--causing bacteria, alpha-hemolytic streptococci, anaerobes are involved in the post- extraction bacteremia. they are of clinical importance because they can cause abscesses in the various parenchymatous organs such as the liver, lung, and brain through anachoresis. the purpose of this study is to establish the types of species of anaerobes associat ... | 1990 | 2133345 |
| comparative in vitro activity of the new erythromycin derivative dirithromycin against gram-positive bacteria isolated from cancer patients. | the in vitro activity of dirithromycin (ly-237216), a new macrolide erythromycin derivative, was compared to that of four other agents (clarithromycin, erythromycin, roxithromycin, clindamycin) against 334 gram-positive isolates obtained from cancer patients. dirithromycin was similar in potency and antimicrobial spectrum to the other agents tested. it was very active against beta-haemolytic streptococci and streptococcus pneumoniae, and moderately active against penicillin and methicillin susce ... | 1990 | 2137413 |
| relation between locomotion, chemotaxis and clustering of immune cells. | experiments were designed to discover whether locomotor or chemotactic events are needed for clustering of lymphocytes with accessory cells or, conversely, whether clustering precedes the activation of lymphocyte locomotion. the time-courses of clustering and locomotor activation were compared and the behaviour of moving cells during cluster formation was filmed. human lymphocytes direct from blood were activated by culture for 24-48 hr with anti-cd3 antibody or in allogeneic mixed leucocyte rea ... | 1990 | 2138123 |
| cyclophosphamide and abrogation of tumor-induced suppressor t cell activity. | previously we have demonstrated that the in vitro generation of p815-specific anti-tumor cytotoxic t lymphocytes (ctl) was suppressed by splenic suppressor t cells from late tumor-bearing hosts (tbh). suppression is not caused by in vitro growth of p815 from splenic metastases, since suppression was also seen with spleen cells from late tbh mice bearing a hypoxanthine/aminopterin/thymidine-sensitive subline (phs-5) of p815 in the presence of hat. cyclophosphamide has been shown to inhibit the in ... | 1990 | 2138931 |
| in-vitro studies with ramoplanin (mdl 62,198): a novel lipoglycopeptide antimicrobial. | ramoplanin is a novel lipoglycopeptide antimicrobial complex, isolated from the fermentation products of a strain of actinoplanes sp. (atcc 33076), which comprises three closely related polypeptides, each containing chlorinated phenyl moieties and d-mannose. the in-vitro activity of ramoplanin was compared with those of vancomycin and teicoplanin. ramoplanin was very active against staphylococcus spp., irrespective of methicillin susceptibility, with all isolates inhibited by 1 mg/l or less. con ... | 1990 | 2139436 |
| [lung pseudotumor caused by corynebacterium equi in an aids patient]. | | 1990 | 2141135 |
| [sensitivity curves of multiply resistant corynebacteria jk and d2]. | corynebacteria groups jk and d2 are opportunistic pathogens. they are sometimes responsible for severe infections, especially in immunocompromised patients. they are resistant to many antibiotics. under these conditions it seemed important to discover which antibiotics among those regularly active, have a killing effect, what their bactericidal speed is, and how they react to combination with other antibiotics. glycopeptids have a killing effect within 24 hours. pristinamycin, rifampin, fusidic ... | 1990 | 2142275 |
| binding of monomeric immunoglobulins by bone marrow-derived mononuclear phagocytes; its modulation by interferon-gamma. | the ability of resting and activated rat bone marrow-derived mononuclear phagocytes (bmm phi) to bind monomeric rat, mouse, and human igg was determined by means of flow cytometry. rat igg2b bound with high affinity (kd approximately equal to 3 x 10(-9) m); binding was optimal at 4 degrees c and was only little affected by trypsin treatment. the other igg bound with only low affinity (rat igg2a, mouse and human igg) or not at all to rat bmm phi (rat igg1, rat igg2c). the binding of rat igg2b was ... | 1990 | 2145176 |
| plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria. | a new shuttle vector pcem500 replicating in escherichia coli and in brevibacterium flavum was constructed. it carries two antibiotic resistance determinants (kmr/gmr from plasmid psa of gram-negative bacteria and smr/spr from plasmid pcg4 of corynebacterium glutamicum) which are efficiently expressed in both hosts and can be inactivated by insertion of dna fragments into the unique restriction endonuclease sites located within them. this vector was found to be stably maintained in b. flavum and ... | 1990 | 2148164 |
| characterization of corynebacterium parvum-induced suppressor cells of mouse nk and adcc activity. | treatment of mice with corynebacterium parvum (cp) resulted in a substantial decrease in natural killer activity in the spleen at 10 days. the decrease in cytotoxicity was associated with the presence of splenic nonadherent (na) suppressor cells, capable of inhibiting natural as well as antibody-dependent cellular cytotoxicity (adcc). the nonadherent suppressor cells appeared to be null cells, lacking detectable expression of thy 1, l3t4 (cd4), lyt 2 (cd8), or asialo-gm1 and could be physically ... | 1990 | 2148342 |
| in vitro activity of ly264826, a new glycopeptide antibiotic, against gram-positive bacteria isolated from patients with cancer. | the in vitro activity of ly264826, a novel glycopeptide antibiotic produced by amycolatopsis orientalis, was compared with those of vancomycin, teicoplanin, and oxacillin against 311 gram-positive clinical isolates from patients with cancer, ly264826 had lower mics for 90% of isolates (mic90) than vancomycin for all species tested. it was active against oxacillin-resistant isolates including staphylococcus aureus (mic90, 0.5 micrograms/ml), staphylococcus haemolyticus (mic90, 2.0 micrograms/ml), ... | 1990 | 2149921 |
| coordinate regulation of siderophore and diphtheria toxin production by iron in corynebacterium diphtheriae. | iron is an environmental signal which regulates the coordinate expression of genes associated with virulence in many pathogenic bacteria. in response to iron-deprivation, lysogenic corynebacterium diphtheriae c7 (beta) synthesizes and secretes diphtheria toxin and siderophore and induces a high-affinity iron uptake system. diphtheria toxin is encoded by beta phage, but genes for siderophore production are encoded on the bacterial chromosome. diphtheria toxin and siderophore production were shown ... | 1990 | 2151460 |
| [development of the vector-host system in corynebacterium. cloning and expression of homoserine dehydrogenase and homoserine kinase genes in corynebacterium cells]. | novel cloning vectors for glutamic acid producing bacteria have been constructed. the cryptic plasmid pbo1 (4.4 kb) from brevibacterium sp. recombined with the plasmid pacyc184 (4.0 kb) from escherichia coli was used to produce composite plasmid named pka1. the plasmid could propagate and express the cm-r phenotype in e. coli and coryneform glutamic acid producing bacteria br. flavum, c. glutamicum, br. lactofermentum. the pka1 plasmid and its variants deleted within non-essential plasmid region ... | 1990 | 2165016 |
| recruitment of 99m-technetium- or 111-indium-labelled polymorphonuclear leucocytes in experimentally induced pyogranulomas in lambs. | the recruitment of polymorphonuclear leucocytes (pmns) during the development of experimental pyogranulomas induced by corynebacterium pseudotuberculosis was followed in nine male lambs by scintigraphic examination. autologous blood pmns were labelled with 99m-technetium or 111-indium and were re-injected intravenously into infected lambs. the functional properties of the labelled cells were monitored 1) in vitro by measuring their phagocytic and bactericidal activity against c. pseudotuberculos ... | 1990 | 2168466 |
| modulation of forssman glycosphingolipid expression by murine macrophages: coinduction with class ii mhc antigen by the lymphokines il4 and il6. | in contrast to murine spleen m phi, resident peritoneal m phi from health mice express very little forssman glycolipid antigen (fo). the following experiments suggest that fo expression by peritoneal m phi may be associated with inflammation. balb/c and cba/j mice were given inflammatory stimuli by i.p. injection of live bcg, thioglycollate (tg), corynebacterium parvum (cp), proteose peptone (pp), or lps. control animals received pyrogen-free saline. expression of fo and ia antigen by peritoneal ... | 1990 | 2168860 |
| diphtheritic polyneuritis in an elderly woman: clinical and neurophysiological follow-up. | a 71-year-old english lady initially presented with a bulbar paralysis and, six weeks later, developed a generalised sensori-motor neuropathy. corynebacterium diphtheriae mitis was cultured from her throat swab. despite a good clinical recovery at one month, nerve conduction velocity was at its lowest. as far as the authors are aware, this is one of the few cases of neurophysiological and clinical follow-up in a british subject with diphtheritic peripheral neuropathy. this case emphasises the im ... | 1990 | 2169838 |
| bacterial flora of the vagina and uterus of healthy cats. | bacterial culturing was conducted on samples from the reproductive tracts of 53 clinically healthy female cats. aerobic bacteria were isolated from 52 of 53 vaginal swab samples and from 2 of 29 uterine swab samples. anaerobic bacteria were detected in 4 of 30 vaginal and 1 of 29 uterine cultures. the aerobic bacteria included species of acinetobacter, actinomyces, corynebacterium, escherichia, haemophilus, klebsiella, lactobacillus, staphylococcus, and streptococcus. coagulase-negative staphylo ... | 1990 | 2179182 |
| pathophysiologic basis for the use of third-generation cephalosporins. | after 10 years of use, the third-generation cephalosporins remain excellent antibiotics. they have superior activity against selected streptococcal species compared with other cephalosporins, and superior activity against haemophilus, neisseria, branhamella, and other less common oral gram-negative aerobic species. despite a very broad spectrum of activity, the third-generation cephalosporins, like all other cephalosporins, have only poor activity against enterococci, listeria, corynebacterium j ... | 1990 | 2183607 |
| differential antibody responses to corynebacterium pseudotuberculosis in sheep with naturally acquired caseous lymphadenitis. | enzyme-linked immunosorbent assays (elisa) with corynebacterium pseudotuberculosis cell wall and bacteria-free supernatant with exotoxin preparations as antigens, and hemolysis inhibition tests were used to detect antibodies in the sera of adult range sheep with naturally acquired caseous lymphadenitis (cl). the extent and severity of lesions were quantitated on the basis of a lesion score, derived from an examination of the carcass (peripheral lymphoid tissue) and viscera (including internal ly ... | 1990 | 2189849 |
| protein g-based enzyme-linked immunosorbent assay for anti-mpb70 antibodies in bovine tuberculosis. | mpb70 is a highly species specific protein which is secreted from mycobacterium bovis during culture. to investigate whether antibodies against mpb70 can be used as an indicator of infection with m. bovis, an enzyme-linked immunosorbent assay was developed, based on the use of biotinylated protein g, to provide a common indicator for antibody formation in different species. during experimental infection with m. bovis in cattle, a characteristic pattern of anti-mpb70 antibody production was obser ... | 1990 | 2191012 |
| evaluation of the worth of corynebacterium parvum in conjunction with chemotherapy as adjuvant treatment for primary breast cancer. eight-year results from the national surgical adjuvant breast and bowel project b-10. | during the 1970s, information obtained from animal tumor models and from patients with a spectrum of solid tumors indicated the worth of a variety of immunostimulating agents. these findings provided a biological and clinical rationale for conducting randomized trials to evaluate the worth of those agents. consequently, in may 1977 the national surgical adjuvant breast and bowel project (nsabp) implemented a randomized trial to determine whether corynebacterium parvum (c. parvum, cp) plus chemot ... | 1990 | 2196108 |
| corynebacteria: incidence among samples submitted to a clinical laboratory for culture. | over the period of one year, 83 corynebacteria isolates were identified in our laboratory, and their clinical relevance assessed by reference to patients whose clinical notes were available. eleven species of corynebacteria were identified including four biotypes of c. jeikeium; six organisms were non-typeable; c. jeikeium and c. xerosis predominated. species identified in the literature as causing clinical infection were also isolated--though in smaller proportions--as were strains of c. jeikei ... | 1990 | 2198419 |
| [antibiotherapy in the patient with granulocytopenic cancer]. | granulocytopenia is the leading alteration of the natural host defense mechanisms, whether caused by an underlying disease or associated with anticancer chemotherapy and radiation therapy. severe granulocytopenia predisposes to septicemia which is now more often due to gram positive than to gram negative bacteria. the empiric therapy of febrile episodes with rapidly bactericidal antibiotics has dramatically modified the prognosis of septicemia. the optimal treatment remains controversial althoug ... | 1990 | 2198526 |
| effects of sucralfate and sulglycotide treatment on active gastritis and helicobacter pylori colonization of the gastric mucosa in non-ulcer dyspepsia patients. | we conducted a double-blind randomized treatment study on patients affects by non-ulcer dyspepsia in whom multiple biopsy specimens showed active gastritis. patients were given either 3 g/day of sucralfate (n = 39) or 600 mg/day of sulglycotide (n = 50) for 6 wk, a glycopeptide isolated from pig duodenum constituents. endoscopy was carried out at baseline and at the end of treatment. we took biopsies from the gastric body (twice) and antrum (six times) at each endoscopy in order to determine gra ... | 1990 | 2202200 |
| detection of diphtheria toxin in culture supernates of corynebacterium diphtheriae and c. ulcerans by immunoassay with monoclonal antibody. | monoclonal antibody (mab) to diphtheria toxin was produced in mouse hybridomas, and shown by elisa to be of sub-class igg1. hybridomas were inoculated into mice to produce ascitic fluid from which mab was purified by caprylic acid. the mab was shown by immunoblotting to be directed against the a fragment of the toxin and also against the intact toxin molecule. after conjugation with fluorescein isothiocyanate, it was used in an immunoassay to detect toxin in culture supernates of corynebacterium ... | 1990 | 2202836 |
| [comparative studies of the detection of corynebacterium suis infections in swine by indirect immunofluorescence and culture]. | in a comparative study 47 urine samples of sows and 9 swabs of preputial diverticulum of boars were investigated for detection of corynebacterium suis (eubacterium suis) using an indirect immunofluorescent technique, a gram stained preparation, a direct culture technique and a culture technique after enrichment. examinations show that the indirect immunofluorescent technique and the enriched culture technique provide the best results, but that the fluorescent technique saves time and expenses. a ... | 1990 | 2206124 |
| in vitro activity of pd127,391, a new quinolone against bacterial isolates from cancer patients. | the in vitro activity of pd127,391, a new 4-quinolone, was compared to that of ciprofloxacin against common clinical bacterial isolates from patients with cancer. pd127,391 was found to have a broad antimicrobial spectrum with excellent activity against gram-positive isolates (including multidrug-resistant organism such as corynebacterium jeikeium, enterococcus faecalis, enterococcus faecium, methicillin-resistant staphylococcus aureus and coagulase-negative staphylococcus spp.). it was also ext ... | 1990 | 2209169 |
| characterization of the antibody response in corynebacterium jeikeium septicaemias. | corynebacterium jeikeium causes septicaemia in neutropenic patients usually after colonizing intravenous lines. this paper reports the results of immunoblotting sera from 14 patients with a c. jeikeium septicaemia. recovery from the septicaemia was associated with production of both igm and igg against antigenic bands of 50, 52 and 110 kda. antibody against the 110 kda band was present in controls but the antibody against the 50 and 52 kda was specific to those patients who had on-going or previ ... | 1990 | 2209731 |
| [endocarditis on prosthetic valve caused by a diphtheroid insensitive to vancomycin]. | | 1990 | 2216016 |
| prevalence of mastitis in imported friesian cows in sudan. | three hundred twenty-two lactating friesian cows were examined for mastitis by different diagnostic techniques. the predominant pathogens encountered were staphylococci, streptococci, corynebacterium and escherichia coli spp. | 1990 | 2218036 |
| [corynebacterium suis infection in swine. 1. clinical diagnosis with special consideration of urine studies and cystoscopy]. | experimental and spontaneous infections with corynebacterium suis in sows were investigated. in early stages animals show no clinical disorders or only for a short time. however, there are already marked changes in urinary samples (hematuria, proteinuria, leukocyturia, gross alterations). using an endoscope mucosal irritations can be seen mainly on the floor of the bladders. in chronic cases alterations in urine are more pronounced. if a pyelonephritis is present in addition to the cystitis, gen ... | 1990 | 2219105 |
| mass spectrometric analysis of o-peracetylated derivatives of 1-monomycoloylglycerol isolated from corynebacterium pseudotuberculosis, rhodococcus rhodochrous and r. lentifragmentus. | 1-monomycoloylglycerols from corynebacterium pseudotuberculosis, rhodococcus rhodochrous and r. lentifragmentus were reacted with acetic acid ahydride in the presence of pyridine. on infrared spectra the reaction products showed a sharp characteristic absorption of the acetyl ester group at 1235 cm-1; the hydroxyl group absorption (3400 cm-1) was absent. o-peracetylated monomycoloylglycerols were analyzed by mass spectrometry under electron impact mode. the most common and representative peaks w ... | 1990 | 2225237 |
| [cloning and expression of the gene for diphtheria toxin and its subunits in escherichia coli]. | the results of cloning corynebacterium diphtheriae phi 984 tox gene and its a and b subunits in escherichia coli are presented. regulatory sequences of tox gene are capable to promote effective expression in e. coli cells. a set of recombinant plasmids has been obtained which can determine the synthesis of a and b individual subunits and are suitable for constructing immunotoxins by gene engineering. the diphtheria toxin of 62 kda synthesized in e. coli has enzymatic activity and reacts with ant ... | 1990 | 2227396 |
| external eye flora as a nutrient source for acanthamoeba. | certain bacteria cause excystment of acanthamoeba from cyst to trophozoite form and are then ingested by migrating trophozoites. we studied the response of acanthamoeba cysts to inoculation on agar seeded with three types of commensal eye bacteria and escherichia coli. amoebae excysted on all bacteria tested, and the migration rate of acanthamoeba trophozoites on each was compared. acanthamoeba migrated with equal speed on e. coli and staphylococcus epidermidis. migration was observed, but was m ... | 1990 | 2227492 |
| efficacy of chlorhexidine as a postmilking teat disinfectant for the prevention of bovine mastitis during lactation. | a natural exposure trial was conducted for 12 mo in a herd of 150 lactating jersey cows to determine efficacy of a .35% chlorhexidine teat dip containing a glycerine emollient for the prevention of bovine intramammary infections. right teats of cows were dipped in the experimental teat dip after milking machine removal and left teats were not dipped. the herd was free of streptococcus agalactiae and had a low prevalence of staphylococcus aureus. most new major pathogen intramammary infections re ... | 1990 | 2229606 |
| modulation of nitrogen oxide synthesis in vivo: ng-monomethyl-l-arginine inhibits endotoxin-induced nitrate/nitrate biosynthesis while promoting hepatic damage. | attempts were made to promote or inhibit nitric oxide (. n = o) synthesis in a murine model of hepatic damage (corynebacterium parvum followed by lipopolysaccharide; lps) to determine the role of . n = o in the liver injury. moderate hepatic damage and increases in circulating no2-/no3- levels were detectable after c. parvum alone. administration of lps to these mice resulted in severe hepatic damage and acute elevations in circulating nitrogen oxide levels. l-arg had no influence on the c. parv ... | 1990 | 2230601 |
| antimicrobial action of roquefortine. | roquefortine, a secondary metabolite of penicillium roqueforti, was investigated with regard to its effect on microorganisms. the growth of gram-positive bacteria was inhibited by roquefortine, whereas gram-negative bacteria were not influenced at all. the minimal inhibitory concentrations for gram-positive organisms were about 80 micrograms/ml cells on the average. the sensitive bacterium corynebacterium flaccumfaciens was chosen for characterizing the antimicrobial action of the mycotoxin. add ... | 1990 | 2231314 |
| [studies on immunity to babesia gibsoni in dogs immunostimulation by bordetella bronchiseptica]. | four species of bacteria, corynebacterium anaerobium 578, actinobacillus pleuropneumoniae g-4, mycobacterium bovis bcg, and bordetella bronchiseptica a-2, were injected intravenously into mice (5 weeks old, icr-spf). the clearance of carbon from the blood stream and the weights of the spleen and liver were determined as indicators of res stimulation. mouse footpad reaction was assessed as an indicator of delayed-type hypersensitivity to each species of bacteria. the immuno-stimulative activity o ... | 1990 | 2232161 |
| antimicrobial activity of naphthoquinones from fusaria. | twenty-two naphthoquinone compounds isolated or derived synthetically from culture extracts of fusarium solani and f. oxysporum were examined for antimicrobial activity. fifteen exhibited antibiotic activity against staphylococcus aureus, and 12 were active against streptococcus pyogenes, but none were active at the highest rate of 128 micrograms/ml against escherichia coli, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, serratia marcescens, or pseudomonas aeruginosa. of 8 plant path ... | 1990 | 2233983 |
| [new aspects of intolerance of the aqueous iodinated x-ray contrast medium, adipiodone--fatal interaction of adipiodone-ultrabil with the immunomodulators corynebacterium parvum (cp) and shigella dysenteriae endotoxin (lps)]. | the authors assume a new type of interaction of the aqueous x-ray contrast substance type adipiodone (ultrabil 50% spofa) with immunomodulators of corynebacterium parvum (cp) and the endotoxin of shigella dysenteriae (lps). the fatal type of interaction developed regularly as a result of the concurrent i.v. administration of the x-ray contrast substance adipiodone with the endotoxin of shigella dys. (lps) to laboratory animals (mice) which had five days previously a single dose of corynebacteriu ... | 1990 | 2237238 |
| endotoxin-induced cytokine gene expression in vivo. ii. regulation of tumor necrosis factor and interleukin-1 alpha/beta expression and suppression. | tumor necrosis factor alpha (tnf alpha) mrna is present in a preformed intracellular pool in the spleen, liver, and small bowel of naive rats. endotoxin (salmonella typhus lipopolysaccharide) injected intravenously induces little or no increase in whole-organ tnf mrna levels at 15', 30', 1 degree, 2 degrees, or 4 degrees, whereas serum tnf levels are markedly elevated at 1 and 2 hours. dexamethasone pretreatment of rats suppresses lps-induced serum tnf concentrations, but does not suppress tnf m ... | 1990 | 2240164 |
| [selective medium for corynebacterium kutscheri and localization of the organism in mice and rats]. | a new selective medium for isolation of corynebacterium kutscheri (ck) from animals suffering subclinical infection was made by adding furazolidone, nalidixic acid and corimycin to the heart infusion agar base, this being named fnc agar. the fnc agar inhibited the growth of gram-negative rods, pseudomonas aeruginosa, proteus sp. and gram-positive cocci but did not affect the growth of ck. when this medium was used to isolate ck from the oral cavity and cecal contents of mice and rats, two of 6 c ... | 1990 | 2242765 |
| corynebacterium pseudotuberculosis in milk of cl affected goats. | | 1990 | 2260259 |
| [characteristics of microbial association in secretions of various mammalian sebaceous glands]. | the following secretions of mammalian skin glands were subjected to a microbiological survey: the middle-abdominal glands of great and mongolian gerbils, the preputial gland of musk deer, the perineal organ of african civet cat, the interdigital skin regions and interdigital glands of bison, european bison, european roe deer, and musk deer. all the secretions were found to contain microbial associations. the composition of bacterial species in the secretions and the quantity of microorganisms va ... | 1990 | 2263223 |
| [infections caused by non-diphtheroid corynebacteria]. | | 1990 | 2263268 |
| nucleotide sequence of the corynebacterium glutamicum trpe gene. | | 1990 | 2263476 |
| immunotherapy of gynecologic malignancies. | systemic corynebacterium parvum and bcg have limited activity in gynecologic malignancies. although intraperitoneal c. parvum is active, its toxicity is prohibitive. intraperitoneal alpha-interferon is an active second line agent for minimal residual disease following combination chemotherapy. intraperitoneal interferon trials are ongoing. alpha-interferon is also active against lower genital tract condyloma acuminata. sufficient numbers of patients have not been evaluated to determine the activ ... | 1990 | 2263813 |
| immunological aspects of mammary tumors in dogs and cats: a survey including own studies and pertinent literature. | naturally occurring cancer in companion animals parallels cancer in man more closely than does experimentally induced cancer in inbred laboratory animals. in dogs and cats, as in man, a role for immune responses is indicated in the development of tumors. a survey is presented based on the literature and our own studies concerning the immunological and immunotherapeutic aspects of canine and feline mammary neoplasia. in dogs bearing mammary neoplasms, circulating immune complexes appear to play a ... | 1990 | 2264285 |
| nucleotide sequence of the replication region from the mycobacterium-escherichia coli shuttle vector pep2. | the replication region derived from the corynebacterium diphtheriae-escherichia coli plasmid png2 was sequenced. this 1.85-kb segment contains a single origin of dna replication, one major open reading frame and shares no sequence homology with previously described plasmids. | 1990 | 2265753 |
| augmentation of host defense against bacterial infection pretreated intraperitoneally with an alpha-glucan rbs in mice. | protection against listeria monocytogenes and escherichia coli in mice was enhanced by an intraperitoneal (i.p.) administration of polysaccharide "rbs". peritoneal macrophages from mice administered i.p. with 30 mg/kg doses of rbs 4 days earlier exhibited increased scavenger functions as assessed by in vivo phagocytosis, in vitro intracellular killing and generation of superoxide anion. when cytokine production of the macrophages was assessed by biological assay and northern blotting analysis, i ... | 1990 | 2266231 |
| urinary tract infection caused by corynebacterium group d2: report of 82 cases and review. | corynebacterium group d2 (cgd2) is a slow-growing, urea-splitting, multiantibiotic-resistant microorganism that is frequently isolated from urine samples and that, in certain circumstances, produces infection of the lower urinary tract (acute and chronic cystitis) and the upper urinary tract (pyelonephritis). this paper analyzes (by means of a retrospective and partially prospective clinical protocol) our experience with 82 patients with cgd2 bacteriuria. the infection was symptomatic in 62% of ... | 1990 | 2267482 |
| caseous lymphadenitis in goats in england. | | 1990 | 2270637 |
| revision of the validity of camp tests for listeria identification. proposal of an alternative method for the determination of haemolytic activity by listeria strains. | the validity of camp tests with staphylococcus aureus and rhodococcus (corynebacterium) equi as defined for listeria identification was revised. this characterization method appeared to be unreliable for two reasons: first, a positive camp test with r. equi is not specific for listeria ivanovii as listeria monocytogenes (and listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with s. aureus when assaying haemolytic and non-haemolytic listeria str ... | 1990 | 2270739 |
| isolation and identification of the gene of cholesterol oxidase from brevibacterium sterolicum atcc 21387, a widely used enzyme in clinical analysis. | the gene coding cholesterol oxidase (chod) from brevibacterium sterolicum, which is widely used in clinical analysis, has been selected from puc19-based gene bank in e. coli mm294 by colony-hybridization using synthetic dna as probe. the gene was identified to encode the protein having the same amino acid sequence as that determined from amino-acid sequence analysis. the expression of the chod gene in e. coli was not observed, probably due to the transcription failure. attempts are being made to ... | 1990 | 2271066 |
| [bacterial translocation from the gastrointestinal tract and endogenous infection induced by immunosuppression after burn]. | this study described the endogenous infections and bacterial translocation from gi tract caused by immunosuppression after burn. in the group of burned plus injected dexamethasone (dxm) (bis, n = 31), the rate of enteric bacteria translocation was 67.4%, the rate of visceral abscess was 65.5%, much more higher than in the group of only dxm (is, n = 15). the translocation of intestinal bacteria also was found in the group of only burned (bu 3/15) and control (ck, 1/23), but endogenous infection d ... | 1990 | 2276052 |
| [immunofluorescence study of intermediate filament protein vimentin of cp-activated macrophages]. | vimentin is an important cytoskeleton protein for the biological function of macrophages. alterations of vimentin filaments of cp-activated macrophages stained with fitc-labeled anti-vimentin antibody were observed under immunofluorescence microscope. activated macrophages showed changes with the following characteristics: the intensity of immunofluorescence of vimentin was increased; the filaments of vimentin became thicker than those of normal macrophages when they were treated with colchicine ... | 1990 | 2279316 |
| isolation and characterization of a restriction and modification deficient mutant of brevibacterium lactofermentum. | in order to facilitate genetic engineering in amino-acid producing bacteria we have isolated two restriction-deficient brevibacterium lactofermentum strains. they have been selected for their ability to obtain a high yield of plaques from cl31 phage which was grown on corynebacterium lilium. these mutant strains do not restrict either phage dna by transfection or dna from the shuttle vector pbla extracted from escherichia coli by protoplast transformation. these mutants have also lost modificati ... | 1990 | 2283029 |
| tnf and inhibition of growth of plasmodium falciparum. | the mechanism of intra-erythrocyte death of plasmodium chabaudi in vivo has not yet been elucidated. here we summarise recent experiments in which serum from mice undergoing a successful immune response to this parasite did not inhibit plasmodium falciparum in vivo unless the p. chabaudi infection and tnf levels were high enough to cause illness in the host. this was true for the 556ka and ds strains of p. chabaudi in intact mice, but not for 556ka in nude mice, which did not generate inhibitory ... | 1990 | 2283147 |
| [immunotoxins]. | an immunologic approach besides surgical, radiotherapeutic, chemotherapeutic methods in the treatment of cancer was proposed for the first time by paul ehrlich in 1906. the structure, produced by conjugating the antibodies against the tumor cells with a toxin molecule by moolten and cooperband (1970) was named as immunotoxins. producing antibodies with the desired amount and purity was achieved by improving the hybridoma technology, immunotoxins were tried to be produced by conjugating the monoc ... | 1990 | 2283968 |
| the influence of various factors on the human resident skin flora. | various factors, e.g. prolonged occlusion, skin disinfectants, systemic antimicrobials, can have an impact on the ecosystem of the normal human skin flora for a short time. these impacts are of clinical importance in the treatment of some skin diseases, where members of the normal human skin flora are involved in the pathogenesis of the disease, e.g. propionibacterium acnes in acne vulgaris, corynebacterium species in erythrasma, trichomycosis palmellina and pitted keratolysis, pityrosporum orbi ... | 1990 | 2285575 |
| bolo disease: a specific, localised skin disease of woolled sheep. | bolo disease is limited to merino and döhne merino sheep in the stutterheim and cathcart districts of the eastern cape province. it occurs under natural grazing conditions regardless of the season of the year and the condition of the natural grazing. ewes and wethers are most frequently affected. skin lesions are well-defined, and the corresponding fleece has dark-grey to almost black spots, patches or bands varying in number, size and distribution between individual sheep. the wool in the affec ... | 1990 | 2287007 |
| bolo disease: a bacteriological survey. | a total of 718 sheep, 381 severely and 190 mildly affected with bolo disease as well as 147 visibly unaffected animals emanating from 15 farms in the stutterheim and cathcart districts in the eastern cape were subjected to bacteriological examination of skin surfaces and wool specimens. altogether, 1,168 specimens were examined. these included skin swabs, skin scrapings and wool samples. corynebacterium spp represented 94.4% of the primary isolates in cultures prepared from all specimens and 97. ... | 1990 | 2287009 |
| characterization of two proteins from leishmania donovani and their use for vaccination against visceral leishmaniasis. | two proteins from leishmania donovani, dp72 and gp70-2, have been previously utilized to specifically serodiagnose patients with visceral leishmaniasis. the proteins were shown by elisa and western blotting with monoclonal and polyclonal antibodies to be present in both stages of the parasite. antibodies to gp70-2 recognize in promastigotes multiple discrete bands of similar m.w. which are common to several isolates of l. donovani. the total amount of ag and number of bands observed per isolate ... | 1990 | 2295807 |
| pathogenicity of acanthamoeba and a corynebacterium in the rat cornea. | acanthamoeba keratitis is a sight-threatening disease that is difficult to treat. the development of an animal model is necessary for many of the studies required to improve visual outcome in human patients. a rat model is proposed that is dependent on coinoculation of amebae and corynebacteria into the corneal stroma. the infective dose was determined for a virulent acanthamoeba isolate and was used to screen 17 other isolates, including 7 from the human cornea. a total of 6 were infective in t ... | 1990 | 2297316 |
| effects of activation on lipid and lipoprotein metabolism in murine macrophages. | the effects of activation on lipid and lipoprotein metabolism were examined in resident murine macrophages, inflammatory cells elicited by thioglycolate, primed cells elicited by pyran copolymer, and activated cells elicited by corynebacterium parvum. low density lipoprotein receptors were reduced by 70%, while scavenger receptors were reduced 60% in activated cells. basal cholesteryl ester and triglyceride synthesis were increased fourfold in activated cells, whereas the activity of 3-hydroxy-3 ... | 1990 | 2297348 |
| adherence of skin bacteria to human epithelial cells. | aerobic and anaerobic bacteria isolated from human axillae were tested for their capacity to adhere to buccal epithelial cells, immortalized human epithelial (hep-2) cells, and undifferentiated and differentiated human epithelial cells. in general, both aerobic and anaerobic diphtheroids adhered better to differentiated human epithelial cells than to hep-2 and undifferentiated human epithelial cells (p less than 0.05). mannose, galactose, fucose, n-acetyl-d-glucosamine, and fibronectin were also ... | 1990 | 2298877 |
| native valve endocarditis caused by an organism resembling corynebacterium striatum. | an organism resembling corynebacterium striatum was isolated from the blood of a patient with acute aortic valvular insufficiency and no history of valvular heart disease. at autopsy, histopathologic examination of the aortic valve revealed pleomorphic gram-positive bacilli and destruction of valvular tissue. our isolate differed from other nondiphtherial corynebacteria, including the type strain of c. striatum (atcc 6940), in its ability to reduce nitrite. nitrite reduction may be useful for di ... | 1990 | 2298880 |
| malignant pleural effusions: meaning of pleural-fluid ph determination. | in 36 patients with malignant pleural effusions, we determined the ph and the glucose concentration of the pleural fluid. twenty-one of 36 patients (58.3%) had a low ph (less than 7.30) and 15 had a normal ph (greater than or equal to 7.30; 7.13 +/- 0.12 vs. 7.37 +/- 0.05; p less than 0.0005). the patients with low ph had significantly lower glucose concentrations than those with normal ph (2.7 +/- 1.4 vs. 6.3 +/- 2.9 mmol/l; p less than 0.0005). twenty-one of 34 patients (61.7%) had a glucose c ... | 1990 | 2300387 |
| a case of bovine valvular endocarditis caused by corynebacterium pseudodiphtheriticum. | | 1990 | 2301113 |
| effect of muramyl dipeptide on immunogenicity of corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs. | colostrum-deprived lambs and cf1 mice were vaccinated with water-in-oil emulsion vaccines containing nonviable whole cells (wc) of corynebacterium pseudotuberculosis with and without muramyl dipeptide (mdp). efficacy of vaccines was determined from the survival of mice and lesions in lambs after iv injection of 10(4) colony-forming units of c pseudotuberculosis. in mice, protection was related to the concentration of wc in the vaccine. at 50, 100, or 150 micrograms of wc, protection was good (78 ... | 1990 | 2301830 |
| in vitro susceptibility of selected veterinary bacterial pathogens to ciprofloxacin, enrofloxacin and norfloxacin. | the minimum inhibitory concentrations (mic) of ciprofloxacin, enrofloxacin, and norfloxacin were tested for approximately ten clinical isolates of each of actinobacillus pleuropneumoniae, actinobacillus suis, actinomyces pyogenes, corynebacterium pseudotuberculosis, erysipelothrix rhusiopathiae, haemophilus parasuis, haemophilus somnus, pasteurella haemolytica, pasteurella multocida, rhodococcus equi, streptococcus equi, streptococcus suis and streptococcus zooepidemicus. ciprofloxacin and enrof ... | 1990 | 2306672 |
| organomercurial-volatilizing bacteria in the mercury-polluted sediment of minamata bay, japan. | a total of 4,604 bacterial strains isolated from the sediments of minamata bay and nearby low-level-mercury stations (control stations) were screened for the ability to volatilize mercury from inorganic and organic mercurial compounds. the strains that volatilize mercury from several kinds of organomercurials were found only in the sediments of minamata bay. | 1990 | 2310185 |
| human pulmonary infection with corynebacterium equi. | a 28 year old man with no underlying disease developed a cavity and multiple nodules in the lung from which corynebacterium equi was isolated. he experimented with organic solvents and microorganisms including corynebacterium species for several years. computed tomography of his pulmonary lesions revealed that these nodules were related to the bronchial tree. histologically, the lesions were compatible with nonspecific inflammatory changes. the clinician must suspect the pulmonary infections wit ... | 1990 | 2311747 |
| microbial ecology of interdigital infections of toe web spaces. | the microbial flora of normal and macerated interdigital toe web spaces was qualitatively and quantitatively identified in 77 patients. dermatophyte fungi were recovered from 11% of normal patients compared with a 31% recovery from patients with macerated interspaces. macerated interspaces were characterized by a greater recovery of organisms with pathogenic potential, with staphylococcus aureus recovered from 36% of patients, micrococcus sedentarius in 37%, brevibacterium epidermidis in 54%, co ... | 1990 | 2319017 |
| microbiostatic effect of murine-activated macrophages for toxoplasma gondii. role for synthesis of inorganic nitrogen oxides from l-arginine. | recent studies show the importance of a single amino acid, l-arginine, as a necessary substrate for activated macrophage-mediated cytotoxic activity for tumor target cells and microbiostatic function for cryptococcus neoformans. the present studies were carried out to determine the role of the l-arginine-dependent macrophage effector function on the microbiostatic effects of activated macrophages on the obligate intracellular protozoan, toxoplasma gondii. a guanidino methylated derivative of l-a ... | 1990 | 2319133 |
| diphtheria with a difference--a rare corynebacterium fatality with associated apoptotic cell death. | a case of infection with corynebacterium ulcerans (c. ulcerans), resulted in the sudden death of a previously healthy 73-year-old woman. death from corynebacterium diphtheriae (c. diphtheriae) infection is well-documented. fatalities following infection with c. ulcerans are unreported; this would appear to be the first documented death due to such infection. | 1990 | 2323738 |
| growth of corynebacterium bovis in mammary secretions during physiological transitions of the bovine mammary gland. | an in vitro microassay was used to evaluate growth of five strains of corynebacterium bovis in mammary secretions collected from quarters of five holstein cows at 0, 14, and 28 d of involution, at parturition, and 14 d after parturition. variation in growth among different strains of corynebacterium bovis was observed. corynebacterium bovis grew well in mammary secretions obtained at the last milking of lactation, at parturition, and 14 d after parturition. however, growth of four strains of cor ... | 1990 | 2329201 |
| catheter-related right-sided endocarditis in bone marrow transplant recipients. | bone marrow transplant recipients are at increased risk of severe central venous catheter-related septicemias that may be complicated by endocardial infection. in view of this, we prospectively evaluated 141 consecutive patients receiving allogeneic or autologous bone marrow infusion. seven (5%) of 141 patients developed eight episodes of a clinical syndrome compatible with catheter-related right-sided infective endocarditis; this diagnosis was confirmed at autopsy in two patients who died. stap ... | 1990 | 2330480 |
| ringlike uptake of [18f]fdg in brain abscess: a pet study. | | 1990 | 2335626 |
| caseous lymphadenitis outbreak. | | 1990 | 2336781 |
| efficiency of skin sterilization for a venipuncture with the use of commercially available alcohol or iodine pads. | skin sterilization for a venipuncture is routinely done with commercially available alcohol or iodine pads. selection of the antiseptics, alcohol or iodine, however, in most situations has been made on the basis of very little scientific data. with many patients with granulocytopenia who are undergoing venipunctures, the choice of antiseptic may be an important factor in preventing infections. we investigated two widely and commercially available disinfectant pads, alcohol and iodine, in the eff ... | 1990 | 2337259 |
| mixed haematogenous endophthalmitis caused by candida albicans and cdc fermentative corynebacterium group a-4. | we report a case of mixed haematogenous endophthalmitis in which candida albicans and centres for disease control (cdc) corynebacterium group a-4 were isolated together from an aspirate of vitreous humour. | 1990 | 2337554 |
| corynebacterium xerosis as a cause of community-acquired endocarditis. | | 1990 | 2338091 |
| [monitoring natural killer cell activity in the blood of patients with malignant pleural effusion after intrapleural administration of corynebacterium parvum]. | in a group of 14 patients with a malignant pleural effusion treated by intrapleural administration of corynebacterium parvum, in addition to other treatment of the generalized malignant process the activity of nk cells was investigated before administration, on the 7th and 21st day after instillation of the vaccine. the mean rise of this activity was significant at the 5% level of significance. the activity of nk cells was equally significantly influenced by age. the survival of patients, althou ... | 1990 | 2340567 |