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complementary imaging modalities in the diagnosis of a mycotic aneurysm. 19911752105
arcanobacterium haemolyticum brain abscess in a child (a case report). 19911752643
mastitis in beef cows and its effects on calf weight gain.quarter milk samples from 51 purebred (angus, polled hereford, and simmental) and 69 crossbred (angus x simmental x charolais three-way cross) beef cows were collected aseptically at three times during lactation to determine the prevalence of intramammary infection, milk somatic cell counts (scc), and effects of infection on calf weight gain. quarter infection prevalence was 13.1, 14.9, and 27.5% in early, mid, and late lactation; corresponding cow infection prevalence was 25.8, 29.2, and 54.4%. ...19911752802
immunisation against ovine caseous lymphadenitis: comparison of corynebacterium pseudotuberculosis vaccines with and without bacterial cells.sheep were immunised with corynebacterium pseudotuberculosis vaccines prepared from cell-free toxoid or from toxoid with formalin-killed cells of c pseudotuberculosis added. resistance of sheep to infection was tested 6 months after immunisation by inoculation with caseous lymphadenitis pus. the outcome was assessed 3 months later by slaughter and inspection of the sheep for lesions of caseous lymphadenitis. immunised sheep were adequately protected against infection as shown by a significant re ...19911755781
immunisation against ovine caseous lymphadenitis: efficacy of monocomponent corynebacterium pseudotuberculosis toxoid vaccine and combined clostridial-corynebacterial vaccines.sheep were immunised with corynebacterium pseudotuberculosis toxoid formulated as a monocomponent vaccine with aluminium adjuvant or in combination with 5 clostridial antigens, and also in the combined form with sodium selenate. immunised and control sheep were experimentally infected 16 days after vaccination and slaughtered and inspected after a further 3 months to determine their resistance to infection. all 3 vaccines afforded an equal and high level of protection; 91% of vaccinated sheep ex ...19911755782
immunisation against ovine caseous lymphadenitis: correlation between corynebacterium pseudotuberculosis toxoid content and protective efficacy in combined clostridial-corynebacterial vaccines.groups of sheep were dosed with vaccines containing corynebacterium pseudotuberculosis toxoid combined in varying amounts with 5 clostridial antigens. resistance of the sheep to infection with c pseudotuberculosis was tested at 1, 6 and 12 months after vaccination by infection with pus from ovine lymph glands actively infected with c pseudotuberculosis. the outcome was assessed 3 months after challenge by slaughter and inspection of the sheep for caseous lymphadenitis lesions. protection was dem ...19911755783
immunity to diphtheria in the 3-19 year age group in italy.in italy, immunization with diphtheria toxoid has been compulsory for all newborns since 1939. the last two clinical cases of diphtheria were reported in 1987. during the period 1987-1989, immunity against diphtheria was assessed by neutralization test in a random sample of 1740 healthy subjects 3-19 years old, from five geographical areas of italy. of the total population, 76.5% showed antibody levels considered to be protective (greater than or equal to 0.1 iu ml-1), 17.2% had a relative degre ...19911759506
[the use of the cluster selection method for assessing the status of immunity to controlled infections (measles, diphtheria and tetanus)].the use of the method of cluster selection in mass serological examination makes it possible to obtain statistically significant results with very low expenditures. the coincidence of the results on the number of seronegative children, obtained by the method of cluster selection, with the results of mass serological examination of all children aged 6-7 years in the district (children found to be seronegative to measles constituted 5.5% and 5.0% respectively) is indicative of the statistical sign ...19911759518
amperometric monitoring of bacteria-induced milk acidity using a platinum disc microelectrode.the acidity induced by the action of bacteria in milk samples was monitored amperometrically by using a platinum microelectrode. the measurements were performed directly on commercial packs of milk, stored at 32 degrees c, and were continued for 9-10 d after inoculation. the data were compared with those obtained by measuring the ph of the samples and the results are discussed on the basis of the metabolism of each bacterial species. the effects of the following bacteria were examined: staphyloc ...19911759720
in vitro activity of 79 antimicrobial agents against corynebacterium group d2.corynebacterium group d2 (cgd2) is involved in urinary tract infections in patients with underlying predisposing factors. this microorganism is highly resistant to a number of antimicrobial agents. we tested the activities of 79 antimicrobial agents against cgd2. beta-lactams, aminoglycosides, and macrolides were ineffective. fluorinated quinolones showed irregular activities, ofloxacin being the most active one. doxycycline, rifampin, and mainly glycopeptides (vancomycin and teicoplanin) were t ...19911759839
[the vaccination of children with allergic diseases with reduced doses of adsorbed dt-m anatoxin].during a remission and drug therapy, 71 children suffering from allergic diseases were vaccinated with dt-m toxoid containing different dose of diphtheria (1, 2.5, 5 lf) and tetanus (1, 2.5, 5 ec) antigens. it has been shown that all the children responded to tetanus antigen by adequate serological tests, whereas as regards diphtheria toxoid, the patients immunized with the least dose of dt-m preparation (1 lf + + 1 ec antigens) produced specific antibodies later and at a lower level. during the ...19911762853
[purulent meningitis, caused by corynebacterium xerosis, after spinal anesthesia].a previously healthy 25 year old sportsman is reported who developed corynebacterium xerosis meningitis with coma and seizures after spinal anaesthesia. the adequate therapy (dexamethason, penicillin, ampicillin, mannitol, intensive care, hyperventillation) resulted in a complete recovery. to the authors' knowledge this is the first case of corynebacterium xerosis meningitis and the first bacterial meningitis reported after spinal anaesthesia in hungary.19911766661
purification of prephenate dehydratase from corynebacterium glutamicum by affinity chromatography.prephenate dehydratase has been purified from the wild type strain corynebacterium glutamicum by affinity chromatography. three ligands, l-trp, l-tyr, and l-phe have been tested as well as conditions for elution. l-phe is the most specific ligand: it leads to a purification factor of 11 in one step using step gradients of nacl in tris-hcl buffer at ph 7.5.19911780276
macrophage tumor cell interaction is enhanced by c3 fragments.we tested the capacity of lewis lung carcinoma cells (3ll) to activate the alternative pathway of complement and to bind the c3 fragments on the plasma membrane. c3 fragments were detected by cytofluorometry and by immunoblotting. in time, the fixed c3b molecules were further cleaved into ic3b. the presence of c3b/ic3b on the target enhanced the formation of conjugates with macrophages. in spite of increased contacts, macrophages from tumor bearing mice were not cytotoxic. only preactivated macr ...19911786985
a field trial to evaluate a whole cell vaccine for the prevention of caseous lymphadenitis in sheep and goat flocks.a field trial to evaluate a whole cell vaccine for the prevention of caseous lymphadenitis (cla) in sheep and goats was performed in one goat herd and one sheep flock over a period of three years. in goats, there was a nonstatistically significant trend for fewer cases of cla in the vaccinated animals compared to the controls. in sheep, from six months to 36 months postinitial vaccination, the proportion of vaccinated sheep that developed cla was significantly less (p less than 0.05) than in the ...19911790493
rapid screening for toxigenic corynebacterium diphtheriae by the polymerase chain reaction.the polymerase chain reaction (pcr) was used to discriminate between toxigenic and non-toxigenic isolates of corynebacterium diphtheriae. primers specific to the diphtheria toxin gene were used to amplify a toxin gene fragment from simple boiled-cell preparations. eight recent clinical isolates and four reference strains were tested. the result of the pcr agreed with the traditional toxigenicity assays (the elek test and guinea pig inoculation) in all cases. pcr has several advantages over the e ...19911791203
failure of dietary restriction to enhance cytolytic and chemiluminescent activity of peritoneal exudate cells from ageing rats.investigations into the cytolytic activity of peritoneal macrophages from male sprague-dawley rats (activated in vivo with corynebacterium parvum) utilised sv40-3t3 and l-929 target cells in a 72 h [3h]thymidine release assay. the dietary restricted rats under test were given a measured amount of food from weaning sufficient to maintain their body weights at approximately 50% of age-matched controls fed ad libitum. cytolytic activity was similar in the two dietary groups at both 13 and 21 months ...19911795565
[perspective: new pathogens: branhamella catarrhalis, capnocytophaga turleri, corynebacterium group jk].branhamella catarrhalis is an important pathogen of upper and lower respiratory tract. according to findings in literature, it mostly causes bronchopulmonary infections. capnocytophaga is a newly recognized genus of the gram negative bacilli. in various reports, it has been recognized that capnocytophaga mostly causes sepsis. bacteria of corynebacterium group jk (cjk) cause many important local infections and sepsis.19911795663
selective medium containing fosfomycin, nalidixic acid, and culture supernatant of rhodococcus equi for isolation of corynebacterium pseudotuberculosis.fnr medium containing fosfomycin, nalidixic acid, bovine blood and culture supernatant of rhodococcus equi was prepared by the present authors, and the medium did not inhibit growth of corynebacterium pseudotuberculosis but completely hampered the growth of bacillus subtilis, staphylococcus aureus, and escherichia coli. the culture supernatant of r. equi facilitated detection of suspected colonies of c. pseudotuberculosis due to synergistic hemolysis. rate of isolation of the organisms (from the ...19911796687
[new bacterial microorganisms in the etiology of human infections. the genus gardnerella].haemophilus vaginalis first affiliated in the bacterial systems in the genus haemophilus, the corynebacterium due to its morphophysiological characteristics--forms a new genus gardnerella with only one species g. vaginalis. the bacterium is important for man's pathology, as it is responsible for the most numerous vaginitis, considered nonspecific. the paper reports synthetically the present knowledge in biology, pathogenicity, diagnosis and treatment.19911802287
corynebacterium cdc group a-4 native valve endocarditis.a patient with endocarditis caused by corynebacterium cdc group a-4 is described. this is the first case in the literature of endocarditis caused by this bacteria, and is unique in that the patient was immune competent and the infection occurred on a native valve. this case illustrates that corynebacteria cannot be considered a contaminant and that the exact pathogen should be identified.19911802696
normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta).the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ...19911803013
[corynebacterium d2 as a ureolytic organism: report of 5 cases].corynebacterium d2, a saprophytic microorganism of skin, causes alkaline encrusted cystitis in patients with a previous bladder injury. in 5 patients that had presented this nosological entity, these gram-positive rods were isolated in urine cultures and calculi. four patients had undergone urological instrumentation maneuvers and one patient (female) had a history of recurrent cystitis from gram-negative bacteria. corynebacterium d2 grows slowly and under certain conditions, as those described ...19911807208
[potential vectors for molecular cloning in brevibacterium flavum].construction of the shuttle cloning vectors for escherichia coli-brevibacterium flavum system is described. expression of the sp/sm resistance determinant derived from the corynebacterium plasmid pcg4 was registered in escherichia coli cells. the genetic determinant for sp/sm resistance was shown to be located in a 2.2 kb psti-sphi fragment by the deletion analysis mapping in escherichia coli cells. using escherichia coli as a host we cloned the unique 0.8 kb ecori-ecori fragment of brevibacteri ...19911808511
[non-diphtheria corynebacterial infections. 7 cases]. 19911808786
vaccine-preventable respiratory infections in childhood.in the past decade, immunization rates among preschool-age children in the united states have decreased to levels lower than those in many developing countries. as a result, epidemics of vaccine-preventable diseases have occurred, especially in urban areas. six of the infections prevented by immunization--those caused by bordetella pertussis, streptococcus pneumoniae, haemophilus influenzae type b, corynebacterium diphtheriae, measles virus, and influenza virus--frequently cause respiratory trac ...19911809999
comparative in vitro activity of the new glycopeptide sk&f 104662 against problematic gram-positive bacteria.the in vitro activity of the new glycopeptide antibiotic sk&f 104662 against problematic gram-positive microorganisms was determined using the agar dilution technique. for comparison, ampicillin, oxacillin, vancomycin, erythromycin and gentamicin were tested. sk&f 104662 was found to have high activity against staphylococcus aureus, coagulase-negative staphylococci (both methicillin sensitive and resistant strains), enterococcus faecalis, enterococcus faecium, listeria monocytogenes, corynebacte ...19911810738
[the adhesive activity of diphtheritic strains in relation to the characteristics of the infectious process they induce].the degree of adhesiveness of 602 c. diphtheriae strains isolated from patients with different forms of diphtheria was studied on trypsinized sheep red blood cells (srbc) used as an experimental model. the titer of bacterial suspension, i.e. its highest dilution ensuring the agglutination of 50% of srbc, was assumed to be the index of adhesive activity. the toxigenic strains were more homogeneous with respect to the degree of their activity and proved to be moderately and highly adhesive, while ...19911811384
cutaneous diphtheria. 19911816125
isolation of corynebacterium renale from slaughtered cattle at the zaria abattoir in nigeria.forty urinary bladders were collected from apparently healthy cattle slaughtered at the zaria abattoir. twenty-four (60%) were from male animals and 16 (40%) from females. urine samples were obtained and cultured for corynebacterium renale. four (16.7%) of the samples from males and 1 (6.3%) from females were positive. the difference in infection between the sexes was statistically significant (p less than 0.001).19911818361
pharmacokinetics of butyric acid derivative with xylitol.the short chain fatty acids, especially butyric acid salts have interesting biological properties. in some cases, transformed cells can recover a normal phenotype and in animal, butyrate salts increase antitumor resistance. butyrate may be considered as possibly useful for antitumor therapy. but these products exhibit two essential disadvantages which restrict their clinical use in man: high concentrations required to achieve therapeutic effects and rapid excretion with short half life. in order ...19911820907
[bacteremic pneumonia caused by rhodococcus equi and hiv infection. report of a new case and review of the literature].rhodococcus equi (corynebacterium equi) is able to produce infections not only in animals but also in patients, usually immunosuppressed ones. we report a new case of bacteremic pneumonia in a drug addict who was also infected by hiv. x-ray film of the chest showed a cavitated infiltrate in right upper lobe. r. equi was recovered from blood, respiratory secretions and lung tissue. the patient was with a prolonged course of antibiotics and also surgical treatment. in spite of both therapies, the ...19911822154
relationship of biotype and source to the hemagglutination and adhesive properties of c. diphtheriae.1. a study was conducted on the hemagglutinating and adhesive properties of sucrose-fermenting and non-sucrose-fermenting corynebacterium diphtheriae strains. sheep erythrocytes were agglutinated by 72% of toxigen c. diphtheriae strains and by 87% of nontoxigenic strains, by 67% of the strains isolated from throats and 94% of skin isolates, and by 65% of sucrose-fermenting organisms and 93% of non-sucrose-fermenting strains. 2. neither presence nor absence of toxin production was correlated with ...19911823253
application of uniform design in l-isoleucine fermentation.uniform design was employed in our experiments for testing the effects of nine different compositions of the culture media for the production of l-isoleucine in corynebacterium crenatum a11 fermentation. the most favorable medium was found to be composed of glucose 12%, (nh4)2so4 2%, kh2po4 0.1%, k2hpo4 0.45%, mgso4.7h2o 0.055%, biotin 1 microgram, thiamine 20 micrograms, feso4.7h2o 2 ppm, mnso4.4h2o 2 ppm, caco3 4% with ph 7.0-7.2. by using such medium, the amount of l-isoleucine produced by a1 ...19911823590
characterization of the natural deletion mutant of plasmid pxz10145 in corynebacterium glutamicum and construction of a recombinant plasmid.plasmid pnat65, carrying the chloramphenicol resistance marker, was chosen from a number of natural deletion mutants of pxz10145 when pxz10145 dna, originally isolated from corynebacterium glutamicum 1014-6t, was used to transform the protoplast of corynebacterium crenatum t6-13. the size of pnat65 was 2.4 kb, determined by electrophoresis on 0.7% agarose gel. the physical map of plasmid pnat65 was determined. one recombinant plasmid, pnar67, was constructed with the dna fragments of pnat65 and ...19911824239
iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned corynebacterium diphtheriae repressor gene dtxr in c. diphtheriae c7 strains.a regulatory gene (dtxr) responsible for iron-dependent repression of the toxin (tox) and siderophore genes in corynebacterium diphtheriae was cloned and characterized. a dna fragment carrying dtxr repressed expression of a tox-lacz gene fusion in escherichia coli dh5 alpha in a high-iron environment but not under low-iron conditions. a protein with mobility corresponding to approximately 28 to 29 kda was identified as the product of the dtxr gene by sodium dodecyl sulfate-polyacrylamide gel ele ...19911828057
antimicrobial susceptibility of corynebacterium pseudotuberculosis isolated from lesions of caseous lymphadenitis in sheep in hokkaido, japan. 19911830812
lymphocyte subpopulations in pyogranulomas of caseous lymphadenitis.pyogranulomas of ovine caseous lymphadenitis (cla) are encapsulated lesions resulting from infections with corynebacterium pseudotuberculosis, a bacterial pathogen able to grow within macrophages. immunohistology of cla lesions showed a band of lymphocytes lining the inside of the collagen capsule in intimate contact with necrotic tissue, the intracapsular lymphocytes being organized into three layers. the innermost layer, immediately adjacent to the central necrotic tissue consisted of a narrow ...19911833096
ifn-gamma modulates the early development of th1 and th2 responses in a murine model of cutaneous leishmaniasis.resistance to leishmania major in mice is associated with the generation of distinct cd4+ th subsets, termed th1 and th2. to define the factors contributing to the genesis of these th cells, we first investigated when these subsets developed following l. major infection. lymph node (ln) cells collected 3 days after infection of balb/c mice secreted il-4 and il-5 in vitro, but little ifn-gamma, whereas ln cells from a resistant strain, c3h/hen, secreted ifn-gamma and no il-4 or il-5. cytokine pro ...19911833466
[the effect of nematode invasiveness in children on postvaccinal immunity]. 19911836830
treatment of encrusted cystitis caused by corynebacterium group d2 with norfloxacin, ciprofloxacin, and teicoplanin in an experimental model in rats.oral doses of norfloxacin (80 mg/kg of body weight per day) and ciprofloxacin (25 and 80 mg/kg/day) and intramuscular doses of teicoplanin (5 mg/kg/day), all administered once a day for 10 days, were evaluated as a means of preventing encrusted cystitis caused by corynebacterium group d2. zinc disks dipped into a 24-h broth culture of these microorganisms were inserted into the bladders of female wistar rats, and treatment was started 14 days after bacterial challenge. the appearance of encruste ...19911839760
effect of tween 80 and dimethyl sulfoxide on biosynthesis of l-lysine in regulatory mutants of corynebacterium glutamicum.by using dimethyl sulfoxide or tween 80 (1 or 0.2%), the production of l-lysine was increased by 20-28 and 23-25%, respectively, in regulatory mutant strains of corynebacterium glutamicum. the stimulation observed is supposed to be caused by influencing cellular surface structures.19911841877
[corynebacterium g-1 infection: description of a case of endocarditis].here we report a case of corynebacterium g1 group endocarditis following acute bpn in non immunodeficient host. the bacterial strain was isolated from the surgically removed heart valve. to our knowledge this is the first report of such an isolate. recently recurrent peritonitis in capd were also ascribed to the same agent.19911844914
construction and characterization of promoter-probe vectors for corynebacteria using the kanamycin-resistance reporter gene.several multicopy promoter-probe plasmid vectors have been constructed that replicate in brevibacterium lactofermentum and related coryneform amino acid-producing bacteria. transcriptional activity is detected by the expression of a promoter-less aminoglycoside phosphotransferase gene (kan) derived from transposon tn5; expression of this gene confers kanamycin resistance in b. lactofermentum. an efficient transcriptional terminator from the b. lactofermentum trp operon has been inserted upstream ...19911849494
in vitro evaluation of gr69153, a novel catechol-substituted cephalosporin.gr69153 is a c-7 catechol cephalosporin with a broad spectrum of activity against members of the family enterobacteriaceae (mics for 50% of strains tested [mic50s], 0.008 to 0.5 micrograms/ml), staphylococcus aureus (mic50, 4 micrograms/ml), pseudomonas aeruginosa (mic50, 0.25 micrograms/ml), haemophilus influenzae (mic50, 0.03 micrograms/ml), neisseria gonorrhoeae (mic50, 0.03 micrograms/ml), and acinetobacter spp. (mic50, 2 micrograms/ml). potent gr69153 activity was also demonstrated against ...19911854174
lung abscess caused by rhodococcus equi.in the immunocompromised patient, early diagnosis of a lung cavity is essential for appropriate treatment. rhodococcus equi (formerly corynebacterium equi) is a variably acid-fast bacterium that can produce cavitary disease in an immunocompromised host. the two cases presented here demonstrate the clinical and radiographic features of r equi lung abscess. the first patient was on long-term corticosteroid therapy for rheumatoid arthritis. the second patient had aids. the correct diagnosis in both ...19911856905
construction and characterization of a mycobacterium-escherichia coli shuttle vector.we have constructed a set of novel mycobacterium-escherichia coli shuttle vectors using either a kanamycin- or hygromycin-resistance gene and the replication region from a corynebacterium plasmid. important features of these new vectors (pep2 and pep3) are that they are small, contain multiple cloning sites, and replicate to high copy number in various mycobacterium species and e. coli. these vectors are unusual in that plasmid replication in gram-negative and gram-positive bacteria appears to b ...19911857754
the effect of nonspecific immune stimulation on wound healing. 19911862151
newly documented antimicrobial activity of quinolones.the improved antimicrobial activity of newer fluoroquinolones and novel applications recently found for the drugs already marketed are reviewed. several new compounds are more active against gram-positive bacteria than the presently marketed fluoroquinolones. win 57273, the most potent compound in vitro on a weight basis, is 16 to 128 times more active than ciprofloxacin against various staphylococci, streptococci, enterococcus spp., corynebacterium spp., listeria monocytogenes and bacillus spp. ...19911864284
a family of corynebacterium glutamicum/escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing.a new family of vectors including cloning vectors (pek0; pec5), an expression vector (pekex1), and promoter probe vectors (pekpllacz; pekplcm), has been constructed. all these shuttle vectors are based on the replication origins of the corynebacterial pbl1 and the escherichia coli cole1 plasmids, and thus are able to replicate in corynebacterium glutamicum and e. coli. plasmids pek0 and pec5 carry multiple restriction sites useful for gene cloning and the kanamycin- or chloramphenicol-resistance ...19911864513
[oral bacterial flora and its pathogenic potential].the oral flora is a complex body formed by at least a hundred bacterial species which can be more or less regular residents in the oral cavity. thanks to a developing taxonomy related to progress made in bacterial identification, these oral species can be separated into five groups based gram staining and morphology: the gram positive cocci group (streptococcus, staphylococcus and related geni), the gram negative cocci group (neisseria, veillonella and related geni), the gram positive bacilli gr ...19911864855
nosocomial infections of ocular conjunctiva in newborns delivered by cesarian section.colonization of the ocular conjunctiva in newborns delivered by cesarian section occurs usually within the first day of life. we have studied the flora of the ocular conjunctiva at birth, from 19 newborns delivered by cesarian section, coming from two different maternity hospitals. ocular conjunctiva cultures yielded the main predominant flora in both maternity hospitals considered. the most common genus of this flora are: staphylococcus, corynebacterium and propionibacterium acnes. peptostrepto ...19911870845
application of quadferm+ for the identification of fastidious gram-positive and gram-negative bacilli.the 2-hr quadferm+ kit (qf) (analytab products, plainview, ny) was compared with conventional tube tests for the identification of the hacek bacteria (haemophilus aphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, kingella kingae), other haemophilus and kingella spp., capnocytophaga, corynbacterium, and moraxella spp. test results were identical for 296 comparisons with 74 isolates (74%). in the remaining 104 comparisons for 26 isolates, 50 reactions ...19911873978
selective medium for isolation of arcanobacterium haemolyticum and streptococcus pyogenes.sheep blood agar using tryptic soy agar base and containing 3.5% sodium chloride was found to facilitate isolation of arcanobacterium haemolyticum and improve isolation of voges-proskauer negative group a (streptococcus pyogenes) and group c and g beta-haemolytic streptococci. commensal oropharyngeal flora such as viridans streptococci, neisseria species, non-haemolytic streptococci and beta-haemolytic streptococci not belonging to groups a, b, c or g were significantly inhibited.19911874249
n-acetyl-beta-d-glucosaminidase, etiologic agent, and duration of clinical signs for sequential episodes of chronic clinical mastitis in dairy cows.this study examined effects of repeated episodes of clinical mastitis in chronically infected quarters on milk n-acetyl-beta-d-glucosaminidase activity and duration of clinical signs. milk samples were collected at each clinical onset from 49 chronic mastitis cases on a 1700-cow michigan dairy farm. there were 49 first episodes of clinical mastitis, 49 second episodes, and 13 episodes of third or more. agents isolated were staphylococcus aureus (18.4%), staphylococcus (7.3%), no growth (20.2%), ...19911880262
l-arginine-dependent macrophage effector functions inhibit metabolic activity of mycobacterium leprae.recently, l-arginine has been shown to be a necessary substrate for murine-activated macrophage-mediated tumor cytostasis and microbiostasis of certain fungi, bacteria, and intracellular protozoa. we report here the effects of the l-arginine-dependent pathway of activated mouse macrophages (mo) on the obligate intracellular prokaryote, mycobacterium leprae. due to the inability to culture m. leprae in vitro, a simple, quantitative assay was employed to measure the metabolism/viability of m. lepr ...19911880420
[refractory hiccup after intrapleural injection of corynebacterium parvum]. 19911882165
genetic control of degradation of chlorinated benzoic acids in arthrobacter globiformis, corynebacterium sepedonicum and pseudomonas cepacia strains.the strains of arthrobacter globiformis kzt1, corynebacterium sepedonicum kz4 and pseudomonas cepacia kz2 capable of early dehalogenation and complete oxidation of 4-chloro-, 2,4-dichloro-and 2-chlorobenzoic acids, respectively, have been analyzed for the origin of the genetic control of degradation. the occurrence and molecular sizes of plasmids in all the strains have been established. plasmid pbs1501 was shown to control 4-chlorobenzoate dehalogenation in the case of kzt1 strain. the same pos ...19911884993
corynebacterium minutissimum infection.two cases of infection due to corynebacterium minutissimum are described. on the basis of biochemical tests the organisms were thought at first to be corynebacterium jeikeium. methods of distinguishing between these species and the role of c. minutissimum in the pathogenesis of erythrasma and other skin infections are discussed.19911885917
[enzootic otitis in a herd of calves].following an outbreak of enzootic bronchopneumonia, 15 out of 19 calves of one herd were affected by uni- or bilateral otitis media. the animals (aged four days to nine weeks) were housed in individual boxes. type and distribution of concurrent diseases as well as the symptoms indicative of otitis are listed in two tables; two other tables give the results of the bacteriological examinations of aural and nasal swabs taken before and during treatment. in order to cure otitis media, systemic as we ...19911887440
infections with rhodococcus equi in children.three cases of serious infection in children, including the first two reports of bacteremia, due to rhodococcus equi are described. only seven pediatric cases have been reported to date. in the laboratory, r. equi can easily be misidentified as a nonpathogenic corynebacterium spp. (diphtheroid) or a mycobacterium spp. despite an overall mortality rate of 25% in adults, no pediatric deaths have occurred due to r. equi. the organism is generally susceptible to vancomycin, aminoglycosides, chloramp ...19911889184
acute natural valve endocarditis due to corynebacterium group 1 in a normal competent host. 19911889453
influence of inoculum volume in diagnosis of environmental mastitis from clinical quarters. 19911892935
coryneform group a-4 endophthalmitis. an experimental animal model.diphtheroids, members of the coryneform family of bacteria, increasingly have been recognized as the cause of serious ocular diseases. after isolation of coryneform group a-4 from two patients with delayed endophthalmitis after cataract extraction and intraocular lens implantation, 10(7) organisms were injected into the vitreous of seven new zealand white rabbits, producing endophthalmitis in all eyes inoculated. coryneform group a-4 subsequently was isolated in six of seven eyes receiving 10(7) ...19911894469
effect of endogenous nitric oxide on mitochondrial respiration of rat hepatocytes in vitro and in vivo.nitric oxide, a highly reactive radical, was recently identified as an intermediate of l-arginine metabolism in mammalian cells. we have shown that nitric oxide synthesis is induced in vitro in cultured hepatocytes by supernatants from activated kupffer cells or in vivo by injecting rats with nonviable corynebacterium parvum. in both cases, nitric oxide biosynthesis in hepatocytes was associated with suppression of total protein synthesis. this study attempts to determine the effect of nitric ox ...19911899557
cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods.cellular fatty acid (cfa) compositions of 561 asporogenous, aerobic gram-positive rods were analyzed by gas-liquid chromatography as an adjunct to their identification when grown on blood agar at 35 degrees c. the organisms could be divided into two groups. in the first group (branched-chain type), which included coryneform cdc groups a-3, a-4, and a-5; some strains of b-1 and b-3; "corynebacterium aquaticum"; brevibacterium liquefaciens; rothia dentocariosa; and listeria spp., the rods had siza ...19911899679
periodic acid-schiff-positive organisms in primary cutaneous bacillus cereus infection. case report and an investigation of the periodic acid-schiff staining properties of bacteria.primary cutaneous bacillus cereus infection frequently presents as a single necrotic bulla on the extremity of an immunocompromised patient. in lesional biopsy specimens and smears, the large gram-positive rods of b cereus may be mistaken for clostridium species. this is a potentially serious error, as bacillus species are resistant to penicillin and other beta-lactam antibiotics. we studied a case in which large periodic acid-schiff-staining organisms were seen in the biopsy specimen from a nec ...19911900984
[diphtheria in denmark 1956-1989. occurrence of corynebacterium diphtheriae and other diphtheria toxigenic bacteria].the public immunization program against diphtheria, established in 1941, has almost eradicated the disease in denmark, and 1956 became the first year without any notified cases. since then, toxigenic strains have only been isolated five times--three cases of clinical diphtheria due to corynebacterium diphtheriae biovar. mitis and two cases of tonsillitis/pharyngitis due to corynebacterium ulcerans. the source of the infection was not identified in any of the cases. the first case of diphtheria i ...19911901182
inflammation near the nerve cell body enhances axonal regeneration.although crushed axons in a dorsal spinal root normally regenerate more slowly than peripheral axons, their regeneration can be accelerated by a conditioning lesion to the corresponding peripheral nerve. these and other observations indicate that injury to peripheral sensory axons triggers changes in their nerve cell bodies that contribute to axonal regeneration. to investigate mechanisms of activating nerve cell bodies, an inflammatory reaction was provoked in rat dorsal root ganglia (drg) thro ...19911901354
further observations on enhancement of the infectivity of fusobacterium necrophorum by other bacteria.it had already been shown with a single virulent strain (a42) of fusobacterium necrophorum that suspension of the fusobacteria in sub-lethal doses of broth cultures of other bacteria reduced the minimum infective dose (greater than 10(6) organisms) for mice by subcutaneous inoculation, sometimes to less than 10 organisms. the present study extended the known range of bacteria with strong infectivity-enhancing properties to include bacillus cereus, klebsiella oxytoca and staphylococcus aureus; an ...19911902184
analysis of a corynebacterium glutamicum hom gene coding for a feedback-resistant homoserine dehydrogenase.from a corynebacterium glutamicum mutant possessing a homoserine dehydrogenase resistant to feedback inhibition by l-threonine, the corresponding gene (homfbr) was analyzed and compared with the wild-type hom gene. dna fragment exchange experiments between both genes showed that a 0.23-kb region close to the 3' terminus of homfbr was responsible for deregulation. nucleotide sequence analysis revealed a single transition from g to a in homfbr leading to replacement of glycine-378 by glutamate in ...19911902466
[use of a phage-plasmid vector for studying the expression of genes coding for the biosynthesis of threonine by corynebacterium glutamicum in the mini-cell escherichia coli system]. 19911902549
network rigidity and metabolic engineering in metabolite overproduction.in order to enhance the yield and productivity of metabolite production, researchers have focused almost exclusively on enzyme amplification or other modifications of the product pathway. however, overproduction of many metabolites requires significant redirection of flux distributions in the primary metabolism, which may not readily occur following product deregulation because metabolic pathways have evolved to exhibit control architectures that resist flux alterations at branch points. this pr ...19911904627
a functionally split pathway for lysine synthesis in corynebacterium glutamicium.three different pathways of d,l-diaminopimelate and l-lysine synthesis are known in procaryotes. determinations of the corresponding enzyme activities in escherichia coli, bacillus subtilis, and bacillus sphaericus verified the fact that in each of these bacteria only one of the possible pathways operates. however, in corynebacterium glutamicum activities are present which allow in principle the use of the dehydrogenase variant and succinylase variant of lysine synthesis together. applying gene- ...19911906065
effect of lactic acid producing bacteria on the human intestinal microflora during ampicillin treatment.20 healthy volunteers participated in a double blind study concerning the effect of lactic acid producing bacteria on the intestinal microflora during ampicillin treatment. 10 volunteers received 500 mg ampicillin tablets t.i.d. together with capsules containing lactic acid producing bacteria (lactobacillus acidophilus and bifidobacterium bifidum) for 7 days, and the other 10 volunteers were given 500 mg ampicillin tablets together with placebo capsules t.i.d. for 7 days. both groups of voluntee ...19911906634
comparison of acellular and whole-cell pertussis-component diphtheria-tetanus-pertussis vaccines in infants. the apdt vaccine study group.in a multicenter, double-blind, randomized, longitudinal study, 252 children received licensed lederle diphtheria-tetanus toxoids and pertussis vaccine adsorbed (dtp) at 2, 4, and 6 months of age, and 245 children received a dtp vaccine with the lederle/takeda acellular pertussis component (apdt) at the same ages. both groups of children received apdt vaccine at 18 months of age. after each of the first three immunizations, apdt vaccine recipients had fewer local and systemic reactions than did ...19911907317
[infections caused by corynebacterium group 2]. 19911907496
microbial lipids and stable foam formation in the activated sludge process.the presence of fats and oils in sewage has been related to the formation of stable foams in activated sludge treatment systems. foam forming microbes can utilise and, in some cases, store lipid substrates. since surface lipids would confer the hydrophobicity necessary for flotation on the sludge biomass, the extractable lipids in foaming and non-foaming biomass samples were examined. both pure mono-cultures and sludge samples were used. the results showed that, whilst there were some difference ...19911907713
rapid confirmation of listeria monocytogenes isolated from foods by a colony blot assay using a digoxigenin-labeled synthetic oligonucleotide probe.an oligodeoxyribonucleotide probe based on the sequence of a 321-bp internal fragment of the msp gene encoding a major secreted polypeptide of listeria monocytogenes was labeled with digoxigenin by using terminal deoxynucleotidyl transferase. the specificity of the digoxigenin-labeled probe was determined by dot blot assays. the probe reacted with all strains of l. monocytogenes tested (12 of 12 strains representing five serotypes). the probe did not react with any other listeria species or with ...19911908203
[the characteristics of corynebacterium diphtheriae adhesins].the study of the properties of c. diphtheriae adhesins revealed the absence of their thermostability, which suggested the protein nature of these adhesins. c. diphtheriae also showed pronounced mannose-resistant hemagglutinating activity, as this activity remained unchanged even in the presence of d-mannose. the comparison of the adhesive activity of c. diphtheriae with their phage and corycine sensitivity revealed essential differences between these macroorganisms in the degree of their adhesiv ...19911909075
microbiology of chronic sinusitis in children.to better understand the factors involved in chronic sinusitis in childhood, we cultured the sinuses, middle meatus, and nasopharynx in 39 children requiring surgical intervention. sixty-nine percent of these patients had other medical problems, including asthma (49%) and immunologic compromise (18%). we cultured coagulase-negative staphylococcus in 18 patients, streptococcus viridans in 14 patients, normal flora in 10 patients, staphylococcus aureus in nine patients, group d streptococcus in fi ...19911910729
alteration in natural defense activity against nk-susceptible b16 melanoma cells after treatment with corynebacterium parvum.we have investigated the effects of administration of c. parvum on host anti-metastatic activity against b16 melanoma h-2l, a natural killer (nk) sensitive clone with a low expression of h-2b. the anti-metastatic activity was estimated by monitoring the following two points. one was the survival ratio at an early stage after an intravenous (iv) inoculation of radiolabeled b16 h-2l cells, the other was the formation of pulmonary metastases after iv injection with the tumor cells. administration o ...19911916884
corynebacterium jeikeium bacteremia at a tertiary care center.during a six-year period 23 patients with isolation of corynebacterium jeikeium (formerly known as corynebacterium group jk) from one or more blood cultures at a university hospital were identified. cases occurred sporadically without time- or ward-related clustering. review of the cases showed that most infections were nosocomial, that most of the patients had underlying malignant disease, had a chronic intravascular catheter implanted, had been pretreated with antibiotics, and were neutropenic ...19911917029
prosthetic valve endocarditis caused by corynebacterium pilosum.a case of prosthetic valve endocarditis caused by corynebacterium pilosum in a 79-year-old woman developed eighty years after aortic valve replacement with bovine pericardium bioprosthesis is described. in spite of the antibiotic therapy she presented an unfavourable course that led to her death.19911917038
cloning, sequencing, and expression of the p-protein gene (phea) of pseudomonas stutzeri in escherichia coli: implications for evolutionary relationships in phenylalanine biosynthesis.the phea gene encoding the bifunctional p-protein (chorismate mutase:prephenate dehydratase) was cloned from pseudomonas stutzeri and sequenced. this is the first gene of phenylalanine biosynthesis to be cloned and sequenced from pseudomonas. the phea gene was expressed in escherichia coli, allowing complementation of an e. coli phea auxotroph. the enzymic and physical properties of the p-protein from a recombinant e. coli auxotroph expressing the phea gene were identical to those of the native ...19911919506
corynebacterium jk: a new pathogen in ventriculostomy infections.in the past decade, corynebacterium jk has emerged as a pathogen in several distinct clinical settings, including sepsis in immunocompromised patients and prosthetic valve endocarditis. it is also recognized as a nosocomial pathogen in infections of prosthetic devices. we present a case of a patient with carcinomatous meningitis who developed a corynebacterium jk infection of an internal ventriculostomy which was used for intraventricular chemotherapy. treatment with systemic and intraventriculo ...19911919648
stability of pathogenic bacteria from laboratory animals in various transport media.the stability of pathogenic bacteria from laboratory animals was investigated in various transport media at different temperatures. bordetella bronchiseptica and salmonella typhimurium survived for 8 days in phosphate-buffered saline (pbs, ph 7.0) at 37, 24, 4 and -20 degrees c; brucella canis at 24, 4 and -20 degrees c; corynebacterium kutscheri at 4 and -20 degrees c; and pseudomonas aeruginosa at all but -20 degrees c. a marked decrease in numbers of pasteurella multocida and past. pneumotrop ...19911921320
septic arthritis caused by corynebacterium xerosis following vascular surgery.a case of septic arthritis due to corynebacterium xerosis occurred after vascular surgery and was successfully treated with intravenous cefotaxime followed by oral erythromycin. the only previously reported case of c. xerosis arthritis developed in a patient with a history of similar recent surgery. the possible association of this infection with such a postsurgical setting is discussed.19911925269
transformation of corynebacterium pseudotuberculosis by electroporation.corynebacterium pseudotuberculosis was transformed by electroporation, using png2, an erythromycin-resistance plasmid from c diphtheriae. corynebacterium pseudotuberculosis cultivated in brain-heart infusion broth was washed 3 times with water, and resuspended to a final concentration of about 5 x 10(13) colony-forming units/ml. an electroporator constructed in our laboratory incorporated an electrode with 0.8-mm interelectrode gap, using disposable spectrophotometer cuvettes as containers for e ...19911928906
polyphasic taxonomic study of the genera gordona and tsukamurella including the description of tsukamurella wratislaviensis sp. nov.representatives of the genera gordona and tsukamurella together with related actinomycetes were the subject of chemotaxonomic, numerical taxonomic and dna homology studies. in the numerical analysis the organisms were examined for 116 unit characters and the data sorted using the simple matching, jaccard and pattern coefficients; clustering was achieved using the unweighted pair group method with arithmetic averages algorithm. the numerical groupings were not affected by the statistics used or b ...19911930572
[endocarditis caused by corynebacterium jeikeium]. 19911932248
lysine excretion by corynebacterium glutamicum. 1. identification of a specific secretion carrier system.corynebacterium glutamicum effectively excretes lysine when the internal lysine concentration is elevated. lysine efflux was investigated using selected mutants which are not able to regulate lysine biosynthesis by feedback inhibition. secretion of lysine is not the consequence of unspecific permeability of the plasma membrane but is mediated by a secretion carrier which is specific for lysine. lysine export is characterized by high activation energy and follows michaelis-menten type kinetics wi ...19911935969
cloning vectors and antibiotic-resistance markers for brevibacterium sp. r312.replication of several cryptic plasmids from coryneform strains was investigated in brevibacterium sp. r312. only the corynebacterium glutamicum psr1 replicon was found to be suitable for establishing a host-vector system. two psr1 derivatives, prpcg200 and phycg1, were used as cloning vectors. they carry a neomycin-resistance-encoding and a tetracycline-resistance-encoding gene, respectively.19911937001
modulation of tumoricidal activity, induced in bone-marrow-derived mononuclear phagocytes by interferon gamma or corynebacterium parvum, by interferon beta, tumor necrosis factor, prostaglandin e2, and transforming growth factor beta.among a series of agents, including various interleukins and growth factors, only interferon gamma (ifn gamma) and heat-killed corynebacterium parvum (cp) organisms were able to elicit, within 24 hr, tumoricidal activity in bone-marrow-derived mononuclear (bmm) phagocytes. in subsequent experiments, the abilities of interferon beta (ifn beta), tumor necrosis factor alpha (tnf alpha), prostaglandin e2 (pge2), and transforming growth factor beta (tfg beta), alone or in combinations of 2, to modula ...19911937965
naftifine treatment of trichomycosis pubis.naftifine hydrochloride 1% cream is a recently developed material of proven efficacy in superficial cutaneous fungal infections. the drug has also been reported to have significant antibacterial properties as well. a case of trichomycosis pubis, a bacterial disorder that can easily be mistaken for several fungal diseases, responded to application of naftifine cream. the authors suggest that this medication may be the drug of choice when the clinical presentation consists of nodules encircling pu ...19911938085
cysteine residues in the active site of corynebacterium sarcosine oxidase.sarcosine oxidase from corynebacterium sp. u-96 is inhibited by iodoacetamide (iam) and the inhibition is prevented by the substrate analog, sodium acetate. to elucidate the mechanism of inhibition of the enzyme by iam, we determined the amino acid sequences around the iam-reactive cysteine residues, and the effects of the modification on the enzyme activity and the oxidation-reduction of the fad moieties of the enzyme. the enzyme was specifically labeled with [14c]iam, and the labeled subunit b ...19911939012
vancomycin.vancomycin is a glycopeptide antibiotic that is active against staphylococci, streptococci, and other gram-positive bacteria. it is the drug of choice for the treatment of infections due to methicillin-resistant staphylococci, corynebacterium jeikeium, and multiply resistant strains of streptococcus pneumoniae. vancomycin is an alternative treatment for serious staphylococcal and streptococcal infections, including endocarditis, when allergy precludes the use of penicillins and cephalosporins. v ...19911943250
antigen specificity and activity of ovine antibodies induced by immunization with corynebacterium pseudotuberculosis culture filtrate.sheep were immunized three times with a vaccine composed of filtrate from a 36 h culture of corynebacterium pseudotuberculosis and a block polymer adjuvant. immunization resulted in the development of exotoxin-neutralizing antibody. this corresponded to the recognition of a 31.6 kda protein on sequential immunoblots of ammonium sulfate-precipitated filtrate. in addition sera from vaccinated sheep recognized at least eight bacterial cellular antigens on immunoblots of ether-extracted c. pseudotub ...19911949570
[the clinico-epidemiological characteristics of diphtheria in adults].of 262 patients referred with suspicion of diphtheria the diagnosis was confirmed in 145 (55.3%). clinico-epidemiological data indicate that the severity of diphtheria course, the tendency of increase of its incidence at the ukrssr territory and in the ussr emphasize that early diagnosis and adequate treatment are of prime importance. to achieve this not only epidemiologists and infectionists but also cardiologists, otolaryngologists and general practitioners should actively participate in manag ...19911949721
airborne microorganism monitoring: a comparison of several methods, including a new direct counting technique.samples of aerosol from the surrounding air were collected by forcing them to impact onto a solid nutrient medium, onto membrane filters, or onto microscope slides on microcover slips. the samples were cultivated or viewed in a fluorescence microscope by using a technique developed by us, or investigated by scanning electron microscopy. the amounts of microorganisms found by cultivation method, i.e. those forming microcolonies (cfu), were on average 85% lower than the amounts determined by the f ...19911950200
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