| the occurrence of bacteraemia associated with the use of oral and nasopharyngeal airways. | in order to determine whether placement of oral or nasopharyngeal airways during anaesthesia induces a significant bacteraemia, 36 asa grade 1 or 2 patients undergoing body surface surgery, in whom it was anticipated that spontaneous respiration would be maintained, were randomly allocated to one of two groups. one group was given oral airways and the other, nasopharyngeal. a series of blood samples, which were taken before and after airway insertion, were cultured aerobically and anaerobically. ... | 1992 | 1539787 |
| inhibition of tumor-specific cytotoxic t-lymphocyte responses by transforming growth factor beta 1. | transforming growth factor beta (tgf-beta) is a potent immunosuppressive cytokine that is produced by neoplastic and normal cells. it has not been demonstrated directly, however, that tgf-beta can inhibit antigen-specific t-cell responses to tumor cells in vitro. we show here that generation of antitumor cytotoxic t-lymphocyte (ctl) activity in mixed-lymphocyte tumor cultures of splenocytes from dba/2 mice immunized with the syngeneic p815 mastocytoma + corynebacterium parvum was consistently an ... | 1992 | 1531782 |
| [corynebacterium spondylodiscitis after diskectomy]. | | 1992 | 1535136 |
| what is your diagnosis? abscess causing lameness in a horse. | | 1992 | 1548177 |
| expression, secretion, and processing of staphylococcal nuclease by corynebacterium glutamicum. | the gene for staphylococcal nuclease (snase), an extracellular enzyme of staphylococcus aureus, was introduced into corynebacterium glutamicum. the heterologous gene was expressed in this host organism, and snase was efficiently exported to the culture medium. amino-terminal sequencing of snase secreted by c. glutamicum revealed that the signal peptide was apparently cleaved off at precisely the same position as in the original host, s. aureus. as with s. aureus, a second smaller form of snase ( ... | 1992 | 1548234 |
| pyometra in camels: case report. | pyometra was recorded in five camels and actinomyces pyogenes (corynebacterium pyogenes) was isolated from pus of the affected camels. | 1992 | 1551020 |
| cloning, organization and functional analysis of ilva, ilvb and ilvc genes from corynebacterium glutamicum. | corynebacterium glutamicum is an industrially important bacterium for the manufacture of amino acids. we constructed genomic libraries of this gram+ bacterium and screened for clones carrying isoleucine biosynthesis genes (ilv) by complementation of escherichia coli mutants. clones complementing ilva, ilvb, and ilvc were isolated. as based on the functional analysis of the corresponding plasmids in c. glutamicum, the dna fragments isolated encode threonine dehydratase, acetohydroxy acid synthase ... | 1992 | 1551588 |
| alkaline-encrusted pyelitis/cystitis and urinary tract infection due to corynebacterium urealyticum: a new severe complication after renal transplantation. | | 1992 | 1539352 |
| propionibacterium acnes-enhanced lens-induced granulomatous uveitis in the rat. | propionibacterium acnes (corynebacterium parvum) is being implicated more frequently as a cause of intraocular inflammation following cataract surgery. in addition to its role as an infectious agent, p. acnes also may possess adjuvant-like or adjuvant-enhancing properties. the presence of this organism in an eye with residual lens material after extracapsular cataract surgery could augment inflammation resulting from a phacoantigenic (phacoanaphylactic) response. we have modified an established ... | 1992 | 1559776 |
| intramammary response to modified intramammary devices. | twenty-five cows in three experiments were used to evaluate the following intramammary devices: abraded surfaces; abraded and sulfur hexafluoride-coated; abraded, sulfur hexafluoride-coated, and weighted; and smooth surfaces and weighted. the objectives of the experiments were 1) to determine whether coating the abraded intramammary devices prevented amorphous deposits and bacterial adherence on the devices, 2) to determine whether addition of weight to devices increased the concentration of som ... | 1992 | 1541746 |
| excretion of glutamate from corynebacterium glutamicum triggered by amine surfactants. | corynebacterium glutamicum is used for the industrial production of glutamate. excretion of the amino acid may be induced by various means. we have analyzed the characteristics of glutamate excretion induced by two amine surfactants, dodecylammonium acetate (da) and dodecyltrimethylammonium bromide (dta). addition of these surfactants induced an immediate efflux of internal glutamate. it also induced a perturbation of the energetic parameters of the cell (decrease of delta mu h, decrease of the ... | 1992 | 1543710 |
| the corynebacterium glutamicum aecd gene encodes a c-s lyase with alpha, beta-elimination activity that degrades aminoethylcysteine. | s-(beta-aminoethyl)-cysteine (aec) resistance was achieved in corynebacterium glutamicum by cloning a chromosomal 1.5-kb ecorv-bglii dna fragment on a multicopy plasmid. dna sequence analysis of the 1.5-kb dna fragment revealed an open reading frame (orf326) which represents the aec resistance gene, designated aecd. the aecd gene directs the synthesis of a 36-kda protein which was visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the aecd gene is a nonessential gene and me ... | 1992 | 1569026 |
| corynebacterium hemolyticum pharyngitis: a cause of airway obstruction. | | 1992 | 1565492 |
| molecular analysis of the corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase. | the corynebacterium glutamicum gdh gene encoding nadp-dependent glutamate dehydrogenase (gdh) has been isolated by complementation of the escherichia coli gdh mutant pa340. the gdh gene was subcloned into the e. coli/c. glutamicum shuttle vector pek0 and introduced into c. glutamicum. recombinant strains showed approximately eightfold higher specific gdh activity (15u mg protein-1) relative to the wild type (1.8u mg protein-1). physiological studies with wild-type and recombinant c. glutamicum s ... | 1992 | 1552846 |
| desulfurization of dibenzothiophene by corynebacterium sp. strain sy1. | strain sy1, identified as a corynebacterium sp., was isolated on the basis of the ability to utilize dibenzothiophene (dbt) as a sole source of sulfur. strain sy1 could utilize a wide range of organic and inorganic sulfur compounds, such as dbt sulfone, dimethyl sulfide, dimethyl sulfoxide, dimethyl sulfone, cs2, fes2, and even elemental sulfur. strain sy1 metabolized dbt to dibenzothiophene-5-oxide, dbt sulfone, and 2-hydroxybiphenyl, which was subsequently nitrated to produce at least two diff ... | 1992 | 1575493 |
| [current status on the epidemiology of acute pharyngitis and post-streptococcal syndromes]. | so far, no ideal study providing an exhaustive knowledge of acute pharyngitis epidemiology has been carried out. what is available now is a number of investigations, all with deficiencies, which concern the duration of the disease (there may be seasonal variations), its limits in space and, above all, the number of pathogens sought for. a well-organized investigation span over at least one year, involve a fairly wide range of age-groups and be repeated in several countries. bacterial epidemiolog ... | 1992 | 1579815 |
| induction of murine lymphokine-activated killer-like cells by corynebacterium parvum (c. parvum) in vitro: lysis of tumor cells and macrophages by c. parvum-induced killer cells. | in vitro culture of murine spleen cells with corynebacterium parvum (c. parvum) was found to induce lymphokine-activated killer (lak)-like cells capable of killing both natural killer (nk)-sensitive and nk-resistant tumor cells as well as syngeneic macrophages (m phi). the induction of lak-like activity by c. parvum was significantly inhibited by anti-interleukin-2 (il-2) or anti-interferon (ifn) alpha, beta antibody (ab), and it was further inhibited by the combination of two abs, suggesting th ... | 1992 | 1580562 |
| [study on immune persistency of combined diphtheria-pertussis-tetanus (dpt) triple vaccine]. | immune efficacy and persistency were studied by comparison of two-dose immunization of adsorbed dpt at one and two month interval and two & three dose immunization of unadsorbed dpt during 1970s and 1980s. serological investigation showed that antibody response to diphtheria, tetanus were elicited in four groups of children after primary immunization, but, the antibody response to pertussis was poor in children immunized with unadsorbed dpt. three kinds of antibody titres were increased signific ... | 1992 | 1581951 |
| influence of corynebacterium parvum-per on disease progression in the nzb/w model of systemic lupus erythematosus. | murine models of systemic lupus erythematosus (sle) exhibit some, but not all, of the characteristics of human disease. disease progression in the animal models is regulated by autoantibodies, genetics and inflammatory processes. in the present study, the influence of the pyridine extract residue of corynebacterium parvum type i (cp-per) on disease progression in the nzb/w model of sle was investigated. cp-per is known to activate the reticuloendothelial (re) system of mice and can alter a numbe ... | 1992 | 1582732 |
| differentiation of corynebacterium spp., listeria spp., and related organisms by using fluorogenic substrates. | a total of 228 strains of arcanobacterium haemolyticum, corynebacterium spp., erysipelothix rhusiopathiae, and listeria spp. were investigated for their abilities to hydrolyze 60 different fluorogenic 4-methylumbelliferyl-linked and beta-naphthylamide-linked substrates within 6 and 24 h of incubation. the hydrolysis of a group of 16 fluorogenic substrates, and in particular, the glycosidase tests, in most cases showed high separation values at the genus level. when used in combination with other ... | 1992 | 1583102 |
| isolation of corynebacterium group d2 from two dogs with urinary tract infections. | corynebacterium group d2 was isolated from two dogs with urinary tract infections. the isolates were resistant in vitro to all tested antibacterial drugs except vancomycin. one dog was successfully treated with this antibiotic, while the other died before treatment could be initiated. | 1992 | 1583115 |
| generation of cytotoxic immune responses against a rat glioma by in vivo priming and secondary in vitro stimulation with tumor cells. | cytotoxic t lymphocyte (ctl) responses to most antigens are generated by in vivo priming and secondary stimulation with antigen in vitro. the present studies were designed to determine whether that strategy could be used to stimulate development of ctl against brain tumors. rats were primed with one of two tumors, rt2, an astrocytoma, or 9l, a gliosarcoma, and corynebacterium parvum. spleen cells from primed rats were stimulated with tumor cells and interleukin-2 in vitro to generate ctl. ctl ge ... | 1992 | 1584347 |
| corynebacterium parvum can reverse the depression of macrophage hydrogen peroxide production caused by erythrocyte phagocytosis. | our previous studies have shown that the phagocytosis of igg-coated erythrocytes (eigg) in vivo increases the mortality rate with bacterial infection, and eigg phagocytosis in vitro depresses phorbol myristate acetate (pma)-triggered h2o2 production. the present study was undertaken to determine if the depression of h2o2 production caused by eigg phagocytosis could be reversed by exposing macrophages to priming agents. macrophages exposed to 100 micrograms/ml of c. parvum, it's pyridine-soluble ... | 1992 | 1587558 |
| the purification, characterization, and primary structure of a small redox protein from methanobacterium thermoautotrophicum, an archaebacterium. | a small redox-active protein has been purified to homogeneity from cell-free extracts of the strictly anaerobic thermophilic methanogen, methanobacterium thermoautotrophicum (strain marburg). the purification consisted of streptomycin sulfate and acid treatments and three chromatographic steps using sephadex g-75, mono q hr 10/10, and superose 12 hr 10/30 columns. when these procedures were carried out under strictly anaerobic conditions, approximately 3 mg of this protein could be isolated from ... | 1992 | 1587836 |
| biofilms on indwelling vascular catheters. | some bacteria have a natural tendency to adhere to available surfaces and to form biofilms. biofilms have been demonstrated on right heart flow-directed catheters, endocardial pacemaker leads, urinary catheters, and other medical devices. in this study, we examined arterial and central venous catheters that had been in place in icu patients between 1 and 14 days for the presence of bacterial biofilms by scanning electron microscopy, transmission electron microscopy, and a special scraping/sonica ... | 1992 | 1572192 |
| the study of agglutination of trypsin-treated sheep red cells by corynebacterium diphtheriae strains. | 620 corynebacterium diphtheriae strains from 472 sick and healthy persons were studied for their adhesive activity (aa) in direct agglutination of trypsin-treated sheep erythrocytes. toxigenic strains had more active aa than non-toxigenic ones which was not dependent on the presence of toxin in the culture. neither biotype nor serotype of the strains correlated with their aa. several lysotypes among toxigenic and non-toxigenic strains were more active than others. toxigenic strains from patients ... | 1992 | 1599709 |
| corynebacterium group d2 infection of a complex renal cyst in a debilitated patient. | | 1992 | 1600022 |
| recurrent infection stones with apparently negative cultures. the case for blind antibacterial treatment. | infection stones in the urinary tract are always associated with infection with a urease-producing, urea-splitting organism. the most common of these organisms are easy to culture and identify and can be treated early either with an appropriate antibiotic or with an anti-urease agent. ureaplasma urealyticum and corynebacterium urealyticum are urease-producing organisms which are difficult to grow; their presence and effects frequently go undetected and untreated. other organisms, as yet unknown, ... | 1992 | 1568095 |
| alternative antimicrobial agents to contemporary treatment of infections caused by corynebacterium parvum (propionibacterium acnes) | | 1992 | 1607339 |
| infection of cerebrospinal fluid shunts in infants: a study of etiological factors. | the aim of this study was to find reasons for the high incidence of cerebrospinal fluid shunt infections seen in neonates. four-hundred sixty-six consecutive shunt operations were analyzed retrospectively in 294 children, and 60 children were studied prospectively by quantitative sampling of skin bacteria before surgery and by sampling open wounds, shunt catheters, surgical gloves, and airborne bacteria. in total, 110 strains of coagulase-negative staphylococcus isolated from the skin of 53 chil ... | 1992 | 1607969 |
| arcanobacterium haemolyticum wound infection. | | 1992 | 1569313 |
| aortic valve endocarditis in a liver transplant recipient--successful management by aortic valve replacement. | | 1992 | 1604494 |
| rational attenuation of corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle. | the phospholipase d (pld) gene (pld) has been deleted from the corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. sheep infection trials indicate that the pld-negative c. pseudotuberculosis strain (toxminus) is incapable of inducing caseous lymphadentis (cheesy gland) even at doses two logs higher than that at which the wild-type strain produces the disease. this clearly establishes pld as a major c. pseudotuberculosis virulence factor. vaccination of sheep with li ... | 1992 | 1612756 |
| the effect of experimental infection with corynebacterium pseudotuberculosis on reproduction in adult ewes. | fifteen ewes were inoculated subcutaneously with 2 x 10(6) cells of corynebacterium pseudotuberculosis seven days before mating, during the embryonic stage of gestation and during the fetal stage of gestation. the clinical signs, antibody response and the consequences of the infection on reproduction were studied. none of the ewes showed any change in general condition during pregnancy. the effects of experimental infection during gestation were evident after incubation periods that ranged from ... | 1992 | 1620958 |
| hemolytic interactions of dermatophilus congolensis. | the strains of dermatophilus congolensis grew on blood agar with washed sheep erythrocytes with marked total hemolysis. in testing for hemolytic interactions they gave a significant synergistic effect of a characteristic shape with rhodococcus equi and streptococcus agalactiae, whereas with staphylococcus aureus producing beta hemolysin and with staphylococcus aureus producing delta hemolysin a simultaneous synergistic as well as antagonistic effect were observed. first of all a conspicuous inhi ... | 1992 | 1621476 |
| clinical reactions and immunogenicity of the biken acellular diphtheria and tetanus toxoids and pertussis vaccine in 4- through 6-year-old us children. | objective--to compare the immunogenicity and reactogenicity of a two-component acellular pertussis vaccine with a whole-cell diphtheria and tetanus toxoids and pertussis vaccine (w-dtp) when administered as a booster to children 4 through 6 years of age. design--this was a randomized, double-blind study. setting--children in this study were from three general pediatric practices (two were private, one was university-affiliated). participants--three hundred and sixteen 4- through 6-year-old child ... | 1992 | 1621656 |
| biological suppression of potato ring rot by fluorescent pseudomonads. | three strains of fluorescent pseudomonads (is-1, is-2, and is-3) isolated from potato underground stems with roots showed in vitro antibiosis against 30 strains of the ring rot bacterium clavibacter michiganensis subsp. sepedonicus. on the basis of morphological and biochemical tests and fatty acid analysis, is-1 and is-2 were identified as pseudomonas aureofaciens and is-3 was identified as p. fluorescens biovar iii. is-1 was the most inhibitory to c. michiganensis subsp. sepedonicus strains in ... | 1992 | 1622275 |
| the role of bacteria in phocine distemper. | the death of many seals believed to be infected with phocine distemper virus was found to be associated with a variety of mainly opportunistic bacterial pathogens. the bacteria most frequently involved were bordetella bronchiseptica, corynebacterium species and a variety of streptococci. seals dying on different parts of the coast of britain were infected with these organisms in differing proportions. | 1992 | 1594938 |
| high-performance liquid chromatography of corynomycolic acids as a tool in identification of corynebacterium species and related organisms. | a high-performance liquid chromatography (hplc) study of 307 strains of corynebacterium species and related taxa revealed that strains classified as "corynebacterium aquaticum"; "corynebacterium asperum"; and centers for disease control (cdc) groups 1, 2, a-3, a-4, a-5, b-1, b-3, e, f-2, and i-2 as well as some unidentified coryneforms do not contain any corynomycolic acids; therefore, they should not be included in the genus corynebacterium. such an hplc method of identification permitted the c ... | 1992 | 1624556 |
| effects of intravesical corynebacterium parvum on recurrences of superficial tumors of the urinary bladder. | we studied 96 patients with superficial tumors of the urinary bladder treated by transurethral resection in order to investigate the value of intravesical corynebacterium parvum (cp) to prevent recurrences. in 52 cases, two vials (2 ml, 4 mg cp) diluted in 60 ml buffer saline were intravesically administered weekly 12 times and then monthly for a period of 2 years. all patients were studied in a 3 year follow-up by cytology, histology and endoscopy. recurrences were observed in 21.1% of cases in ... | 1992 | 1623214 |
| evaluation of the rapid coryne identification system for corynebacterium species and other coryneforms. | the rapid coryne system for identification of aerobic, nonsporeforming, gram-positive rods was evaluated according to the manufacturer's instructions with 177 organisms. after inoculation with a heavy suspension of growth, strips containing 20 cupules were incubated for 24 h, reagents were added, and the results of 21 biochemical reactions were recorded as numerical profiles. the strains consisted of pathogenic species of the genus corynebacterium, primarily c. diphtheriae (n = 29), opportunisti ... | 1992 | 1629322 |
| specific binding of the diphtheria tox regulatory element dtxr to the tox operator requires divalent heavy metal ions and a 9-base-pair interrupted palindromic sequence. | the structural gene for diphtheria toxin, tox, is carried by a family of closely related corynebacteriophages; however, the regulation of tox expression is controlled by a corynebacterium diphtheriae-encoded regulatory element, dtxr. the molecular cloning and sequence analysis of dtxr was recently described. previous studies have suggested that dtxr-mediated regulation of the diphtheria tox operator involves the formation of an iron-repressor complex, which specifically binds to the tox operator ... | 1992 | 1631071 |
| nonspecific stimulation of host defense by corynebacterium kutscheri. iii. enhanced cytokine induction by the active moiety of c. kutscheri. | the present study was carried out to ascertain whether the active component of corynebacterium kutscheri (ck-m) could stimulate host cells of mice to produce several cytokines. ck-m stimulated thioglycollate-induced peritoneal macrophages to produce interleukin 1 (il-1) and tumor necrosis factor alpha (tnf-alpha) at concentrations of 1-100 ng/ml, and it also induced il-2 and interferon-gamma (ifn-gamma) as well as il-6 production by splenocytes. maximum production of each cytokine induced by ck- ... | 1992 | 1611281 |
| human infections with actinomyces pyogenes (corynebacterium pyogenes). | actinomyces pyogenes (corynebacterium pyogenes), a well-known pathogen in many animals, was isolated from 11 danish patients since 1968. bacteriologic characteristics and clinical pictures of the patients are described. ability to hydrolyze gelatine, to produce beta-glucuronidase, to reach with antisera against group-g streptococci, and to produce acid from xylose differentiates a. pyogenes from arcanobacterium haemolyticum, with which it has at times been confused. actinomyces pyogenes is an es ... | 1992 | 1611850 |
| long-chain acyl-coa ester intermediates of beta-oxidation of mono- and di-carboxylic fatty acids by extracts of corynebacterium sp. strain 7e1c. | beta-oxidation of palmitate and tetradecanedioic acid was studied in cell-free extracts of the gram-positive bacterium corynebacterium sp. strain 7e1c, and the acyl-coa ester intermediates formed were analysed by h.p.l.c. beta-oxidation assays displayed a lag phase before a constant rate of nad+ reduction was obtained. the length of the lag phase was inversely proportional to the number of units of activity added to assays. this is a characteristic feature of a system of consecutive reactions pr ... | 1992 | 1637289 |
| cytotoxic activity of a recombinant chimaeric protein between pseudomonas aeruginosa exotoxin a and corynebacterium diphtheriae diphtheria toxin. | a segment of the exotoxin a gene of pseudomonas aeruginosa, coding for the n-terminal end of domain i and domain ii of the toxin (eta), was genetically fused to the diphtheria toxin gene of corynebacterium diphtheriae, coding for the n-terminal end of a fragment of diphtheria toxin (dt). the resulting hybrid protein (termed ced1) was produced in large amounts and exported to the periplasm in escherichia coli. this chimaeric protein reacted with both anti-eta and anti-dt antisera. furthermore, th ... | 1992 | 1640830 |
| diphtheria toxin cloning and expression in corynebacterium diphtheriae. | | 1992 | 1633295 |
| expression of the bacillus subtilis sacb gene leads to sucrose sensitivity in the gram-positive bacterium corynebacterium glutamicum but not in streptomyces lividans. | the expression of the structural gene (sacb) encoding bacillus subtilis levansucrase in two gram-positive soil bacteria, corynebacterium glutamicum atcc 13032 and streptomyces lividans 1326, was investigated. sacb expression in the presence of sucrose is lethal to c. glutamicum but not to s. lividans. while s. lividans secretes levansucrase into the medium, we could show that the enzyme is retained by c. glutamicum cells. our results imply that the sacb gene can be used as a positive selection s ... | 1992 | 1644774 |
| the structure of diphtheria toxin as a guide to rational design. | | 1992 | 1633298 |
| diphtheria toxin and pseudomonas aeruginosa exotoxin a: active-site structure and enzymic mechanism. | | 1992 | 1628498 |
| [the level of postvaccinal immunity to diphtheria, tetanus and poliomyelitis in relation to the number of inoculations]. | the work was aimed at the comparative study of the intensity of immunity to diphtheria, tetanus and poliomyelitis, depending on the number of injections of adsorbed diphtheria-pertussis-tetanus (dpt) vaccine and poliomyelitis vaccine, for the purpose of finding out the possibility of reducing the antigenic load given to children without diminishing the intensity of immunity. to determine the level of immunity to diphtheria and tetanus, 1900 children under school age were serologically studied by ... | 1991 | 1650072 |
| elimination of the disulphide bridge in fragment b of diphtheria toxin: effect on membrane insertion, channel formation, and atp binding. | active diphtheria toxin consists of two disulphide-linked fragments, termed a and b. fragment b, which contains an internal disulphide bridge, facilitates translocation of the enzymatically active fragment a to the cytosol of eukaryotic cells. in this process cation-selective channels are formed. an in vitro translated full-length mutant lacking the internal disulphide bridge (a-58**) was functionally indistinguishable from its disulphide-containing counterpart (a-58) with respect to trypsin sen ... | 1991 | 1646374 |
| quantitative analysis of the epstein-barr virus-inducing properties of short-chain fatty acids present in the culture fluids of oral bacteria. | the culture fluids of various anaerobic bacteria induced the synthesis of early antigens (ea) in epstein-barr virus (ebv) carrying lymphoblastoid cells. the culture fluids of corynebacterium butyricum and fusobacterium nucleatum were the effective inducer on ea. the inducing activity was, to some extent, dependent on their n-butyric acid content, but appeared to be regulated by yet unidentified materials. | 1991 | 1652240 |
| differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with corynebacterium pseudotuberculosis, pasteurella haemolytica, or lentiviruses. | soluble mediators such as tumor necrosis factor alpha (tnf-alpha) may be important in the pathogenesis of many chronic pulmonary infections. we examined the ability of corynebacterium pseudotuberculosis, pasteurella haemolytica, and ovine lentiviruses (ovlv) to induce tnf-alpha secretion by pulmonary alveolar macrophages (pam). bronchoalveolar lavage cells, composed of greater than 90% pam, were obtained from normal sheep. bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in ... | 1991 | 1652561 |
| in vitro activity of ly264826 compared to other glycopeptides and daptomycin. | ly264826 a new naturally occurring glycopeptide inhibited 90% of methicillin-susceptible and -resistant staphylococcus aureus at 1 micrograms/ml. ly264826 had similar activity against methicillin-susceptible and -resistant coagulase-negative staphylococci. the ly264826 mic90 for streptococcus pyogenes was 0.25 microgram/ml, twofold more active than vancomycin and twofold less active than teicoplanin. ly264826 was eightfold more active than vancomycin and twofold more active than teicoplanin agai ... | 1991 | 1651826 |
| lysine excretion by corynebacterium glutamicum. 2. energetics and mechanism of the transport system. | lysine excretion in corynebacterium glutamicum was characterized as secondary transport process. it is modulated by three forces: the membrane potential, the chemical potential of lysine, and the proton gradient. the atp content of the cells did not correlate with the export activity. lysine is excreted in symport with presumably two oh- ions which is not distinguishable experimentally from an antiport mechanism against two protons. the substrate-loaded carrier is uncharged. when the external su ... | 1991 | 1657604 |
| kinetics of electron entry, exit, and interflavin electron transfer during catalysis by sarcosine oxidase. | sarcosine oxidase contains 1 mol of covalently bound plus 1 mol of noncovalently bound fad per active site. the first phase of the anaerobic reduction of the enzyme with sarcosine converts oxidized enzyme to an equilibrium mixture of two-electron-reduced forms (eh2) and occurs at a rate (2700 min-1, ph 8.0) similar to that determined for the maximum rate of aerobic turnover in steady-state kinetic studies (2600 min-1). the second phase of the anaerobic half-reaction converts eh2 to the four-elec ... | 1991 | 1657156 |
| method for in situ evaluation of superoxide production by pulmonary macrophages in the rat. | in order to investigate superoxide production by pulmonary macrophages in the rat, a route was created by ligating both the inferior and superior venae cavae and resecting the aorta after cannulation through the inferior vena cava into the right atrium of the heart. lung perfusion was performed via this route with nitro blue tetrazolium. although there was no formazan deposition throughout the lung, it became detectable in both alveolar and interstitial macrophages when phorbol myristate acetate ... | 1991 | 1648858 |
| 'integron'-bearing vectors: a method suitable for stable chromosomal integration in highly restrictive corynebacteria. | a pbr322-derived plasmid (pcgl107) that carries the corynebacterium melassecola atcc17965 analogue of escherichia coli gdha gene (encoding glutamate dehydrogenase), was introduced into the related strain, brevibacterium lactofermentum cgl2002, by electroporation and integrated into its chromosome by homologous recombination. however, pcgl107 cannot integrate into c. melassecola, since the host restriction prevents successful electroporation by e. coli-modified dna. nevertheless, b. lactofermentu ... | 1991 | 1660430 |
| characterization of pga1, a new plasmid from corynebacterium glutamicum lp-6. | a new plasmid, pga1, has been isolated from corynebacterium glutamicum lp-6, and its detailed restriction map has been prepared. the 4.9-kb plasmid has a g + c content of 57%. it replicates in c. glutamicum atcc13032 and is compatible with the three other plasmids, pcc1, pbl1 and phm1519, commonly used for vector construction for amino acid-producing corynebacteria. fusions of pga1 with different escherichia coli replicons (transferred from e. coli to corynebacterium via transformation of sphero ... | 1991 | 1660431 |
| in vitro activity of sparfloxacin and six reference antibiotics against gram-positive bacteria. | the in vitro activity of sparfloxacin, a new fluoroquinolone, was assessed against 234 gram-positive bacterial isolates by agar dilution (10(4) cfu/spot). sparfloxacin activity was compared with that of ciprofloxacin and five other antibiotics. sparfloxacin was the most active drug tested against methicillin-sensitive and methicillin-resistant staphylococcus aureus (mrsa) and coagulase-negative staphylococci (mic90, 0.125-0.25 mg/l). sparfloxacin was also the most active drug tested against ente ... | 1991 | 1665123 |
| molecular analysis of the corynebacterium glutamicum lysl gene involved in lysine uptake. | two corynebacterium glutamicum mutants defective in lysine uptake were identified by analysing mutants resistant to s-(2-aminoethyl)-cysteine (aec). a 5.6 kb genomic dna fragment restoring aec sensitivity and lysine uptake was isolated. a 4.2 kb subfragment was sequenced and three open reading frames were identified. subcloning and gene disruption experiments showed that only the first open reading frame, termed lysl, is involved in lysine uptake. lysl consists of 501 amino acids with a mr of 53 ... | 1991 | 1667221 |
| toxigenic corynebacterium diphtheriae associated with the haj. | | 1991 | 1669870 |
| isolation of corynebacterium kutscheri from aged syrian hamsters (mesocricetus auratus). | corynebacterium kutscheri was isolated from the oral cavities of 12 male syrian hamsters (mesocricetus auratus) which were about 12 months old. at 1, 5, and 9 months after initial isolation of c. kutscheri from the oral cavity, hamsters were euthanatized, and attempts were made to culture c. kutscheri from 13 additional sites. corynebacterium kutscheri was isolated from nine hamsters, and regardless of the hamsters' ages, the organisms were most frequently isolated from the oral cavity (100%), e ... | 1991 | 1658468 |
| [method and procedures in bacteriological study of necrotic teeth]. | research was conducted of 160 radicular canals with necrotic pulp. results of different bacteriological analyses are presented. culture analyses in aerobic and anaerobic media, resulted in the isolation of staphylococcus epidermidis, streptococcus viridans and corynebacterium sp in the group studied, as the most frequent bacteria. there was no evidence of a specific germ linked with the pulp necrosis. | 1991 | 1659855 |
| multiresistant corynebacteria in bacteriuria: a comparative study of the role of corynebacterium group d2 and corynebacterium jeikeium. | during an 11-month prospective study, urine cultures were performed on 5685 samples obtained in three hospital units. the use of a selective medium improved the recovery of antibiotic-multiresistant corynebacteria (amc): 703 isolates (12.4%) compared with 88 isolates (1.6%) on sheep blood agar. corynebacterium group d2 (cgd2) was isolated in 80.5% of urines yielding greater than or equal to 10(5) amc ml-1 whereas corynebacterium jeikeium represented 80.2% of isolates with less than 10(5) amc ml- ... | 1991 | 1672322 |
| phase variation of pili of corynebacterium pilosum. | whether or not (?) phase variation occurs in the pili of corynebacterium pilosum was biologically examined using the colony enzyme-linked immunosorbent assay (elisa) blot test with anti-pili immune serum. from the densely piliated clone (35p+) of c. pilosum 35, non-piliated variants were isolated at a frequency of 2.45 x 10(-3). from one of the non-piliated variants (designated as p(-11)/35p+), a piliated variant was isolated at a frequency of 4.68 x 10(-4), one log less frequently than the non- ... | 1991 | 1679590 |
| immunologically mediated fatigue: a murine model. | chronic fatigue syndrome (cfs) is an idiopathic disorder in which the chief symptoms is profound fatigue. to explore the relationship between immune stimulation and fatigue, we developed a murine model for quantifying fatigue: reduction in voluntary running and delayed initiation of grooming after swimming. inoculation of female balb/c mice with corynebacterium parvum antigen or the relatively avirulent me49 strain of toxoplasma gondii induced fatigue: baseline running reduced to less than 50 an ... | 1992 | 1643746 |
| discrimination of corynebacterium glutamicum, brevibacterium flavum and brevibacterium lactofermentum by restriction pattern analysis of dna adjacent to the hom gene. | different strains of corynebacterium glutamicum, brevibacterium flavum, and brevibacterium lactofermentum were analysed for restriction fragment length polymorphism using the homoserine dehydrogenase gene (hom) as a probe. the hybridization patterns obtained pvuii- or asp700-restriction of chromosomal dna were specific and distinguishable for each of the three species and identical for the different strains of each species. thus, the method employed allows rapid distinction of corynebacterium gl ... | 1991 | 1682208 |
| mechanism of gram variability in select bacteria. | gram stains were performed on strains of actinomyces bovis, actinomyces viscosus, arthrobacter globiformis, bacillus brevis, butyrivibrio fibrisolvens, clostridium tetani, clostridium thermosaccharolyticum, corynebacterium parvum, mycobacterium phlei, and propionibacterium acnes, using a modified gram regimen that allowed the staining process to be observed by electron microscopy (j. a. davies, g. k. anderson, t. j. beveridge, and h. c. clark, j. bacteriol. 156:837-845, 1983). furthermore, since ... | 1990 | 1689718 |
| adherence and hemagglutination of corynebacterium group d2. | sixty-one strains of corynebacterium group d2 were examined for their ability to adhere to human uroepithelial cells and to agglutinate human and guinea-pig erythrocytes. strains were isolated from samples of two origins: urine of bacteriuric patients and healthy skin of patients without urinary infection. in addition, the isolates were examined by scanning and transmission electron microscopy. heavy adherence to the uroepithelial cells but weak hemagglutination were noted. no statistical associ ... | 1991 | 1672639 |
| routine screening for corynebacterium diphtheriae. | | 1991 | 1678835 |
| [the use of medium 199 in the bacteriological diagnosis of diphtheria]. | | 1990 | 1691349 |
| corynebacterium parvum (propionibacterium acnes): an inducer of tumor necrosis factor-alpha in human peripheral blood mononuclear cells and monocytes in vitro. | the present study investigates the potential capacity of the immunostimulant corynebacterium parvum (c.p.) to induce tumor necrosis factor-alpha (tnf-alpha) in human peripheral blood mononuclear cells (pbmc) and blood monocytes (bmo) in vitro. both at the mrna and protein level, stimulation of pbmc and bmo upon c.p. induces tnf-alpha. compared to the hitherto used tnf-alpha inducers in vitro such as sendai virus, phytohemagglutinin or lipopolysaccharide the c.p. stimulus displayed a threefold st ... | 1990 | 1698632 |
| medical and microbiological problems arising from airborne infection in hospitals. | the practical importance and frequency of airborne nosocomial infections has been a matter of dispute for many years. this is because most of the pathogens acquired in hospitals are able to use various different routes of infecting the patient's body so that it may be difficult or even impossible to prove an individual infection to be airborne. only microbes such as streptococcus pyogenes, neisseria meningitidis, corynebacterium diphtheriae, mycobacterium tuberculosis, or certain respiratory vir ... | 1991 | 1679815 |
| [a case of diphtheria in an adult]. | | 1990 | 1704664 |
| molecular basis of "suppressor" macrophages. arginine metabolism via the nitric oxide synthetase pathway. | a molecular explanation for "suppressor" macrophage inhibition of lymphocyte proliferation is described. ng-monomethyl-l-arginine (ngmma), a specific inhibitor of the nitric oxide synthetase pathway, markedly augments con a-induced proliferation of rat splenic leukocytes. macrophages are necessary and sufficient for ngmma-releasable-suppression, as indicated by a loss of suppression after either pretreatment of isolated splenic macrophages with ngmma or their depletion by plastic adherence or l- ... | 1991 | 1707918 |
| transfer of brevibacterium divaricatum dsm 20297t, "brevibacterium flavum" dsm 20411, "brevibacterium lactofermentum" dsm 20412 and dsm 1412, and corynebacterium glutamicum and their distinction by rrna gene restriction patterns. | the results of dna-dna hybridization and chemotaxonomic studies indicated that the glutamic acid producers brevibacterium divaricatum dsm 20297t (t=type strain), "brevibacterium flavum" dsm 20411, "brevibacterium lactofermentum" dsm 1412 and dsm 20412, corynebacterium lilium dsm 20137t, and corynebacterium glutamicum dsm 20300t and dsm 20163 are members of the same species. it is proposed that all of these strains should be classified in the species corynebacterium glutamicum. another glutamic a ... | 1991 | 1713055 |
| a numerical analysis of ribosomal rna gene patterns for typing clinical isolates of corynebacterium group d2. | restriction digest fragments of dna from 46 clinical isolates identified as corynebacterium group d2, were separated by electrophoresis, southern blotted onto nylon membranes and hybridized to a ribosomal rna gene probe. the resulting band patterns were subjected to unweighed pair-group cluster analysis. representative strains from the main clusters were compared with similarly prepared band patterns from type strains of human corynebacterium species. the results indicate that strains identified ... | 1991 | 1718768 |
| hsp 90, yeasts and corynebacterium jeikeium. | recovery from disseminated candidosis is associated with seroconversion to a 47 kda breakdown product of the heat shock protein (hsp) 90 of candida albicans. cloning, sequencing and epitope mapping has allowed the delineation of the immunodominant epitopes lkvirknivkkmie and stdepagesa. monoclonal and polyclonal antibodies specific to these epitopes are used to show that all strains of c. albicans tested produce hsp 90 in both the yeast and mycelial phases. homologous proteins are demonstrated i ... | 1991 | 1718769 |
| characterization of a defective diphtheria toxin repressor (dtxr) allele and analysis of dtxr transcription in wild-type and mutant strains of corynebacterium diphtheriae. | the production of diphtheria toxin and siderophore by the corynebacterium diphtheriae regulatory mutant c7(beta)hm723 is resistant to the inhibitory effects of iron, and the mutant strain is defective for function of the regulatory gene dtxr. a 2.8-kb hindiii fragment carrying the c7(beta)hm723 dtxr allele was cloned and characterized in escherichia coli. the restriction endonuclease maps of the 2.8-kb hindiii fragment from c7(beta)hm723 and the corresponding fragment from wild-type c. diphtheri ... | 1991 | 1718867 |
| clinical features of the yellow nail syndrome. | the yellow nail syndrome is a rare cause of exudative pleural effusions. diagnosis is based on the presence of typical clinical features, striking nail changes, lymphedema, and pleural effusions of undetermined etiology. there is no direct relationship between the syndrome and primary immunologic disorders, malignancies, endocrine abnormalities, or connective tissue diseases. in patients with symptomatic pleural effusions, pleurodesis is beneficial but no treatment for the nail changes has been ... | 1990 | 1695555 |
| improved vectors for transcriptional signal screening in corynebacteria. | new promoter-probe and terminator-probe shuttle vectors for escherichia coli and corynebacteria were constructed. these vectors, working with the uida gene of e. coli as reporter gene, are very useful for the cloning and subsequent analysis of transcriptional regulatory signals. the beta-glucuronidase encoding activity of uida can be easily detected on agar plates containing chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-beta-d-glucuronide (x-gluc). in the terminator-probe vector put2 ... | 1991 | 1722768 |
| inhibition of nk cell generation by corynebacterium parvum. | treatment of mice with corynebacterium parvum (cp) resulted in a substantial decrease of splenic nk activity associated with a reduced number of lgl. cp also inhibited in vitro augmentation of nk cytotoxicity by ifn or il-2 as well as generation of lak activity. localization experiments by using radiolabelled lgl indicated that the lower number of lgl in the spleen was not attributable to a cp-induced alteration of lgl homing. finally cp was found to affect the ability of bone marrow cells to re ... | 1991 | 1723083 |
| [a serum-free variant of a medium for determining the toxigenicity of corynebacterium diphtheriae]. | | 1990 | 1699074 |
| infections in implantable cardioverter defibrillator patients. | implantable cardioverter defibrillators (icds) have been documented as an effective modality in reducing arrhythmic mortality. a serious complication associated with implantation of the device is infection. few studies have addressed this issue. two hundred seven patients with refractory ventricular arrhythmias underwent 207 icd implantations, and 56 subcutaneous generator changes at our institution. eight patients developed wound infections, four following icd implantation (4 out of 207 or 1.9% ... | 1990 | 1701886 |
| impact of haemophilus influenzae type b polysaccharide-tetanus protein conjugate vaccine on responses to concurrently administered diphtheria-tetanus-pertussis vaccine. | to assess whether serum antibody responses to diphtheria-tetanus-pertussis (dtp) vaccine were affected by coadministration of haemophilus influenzae type b capsular polyribosylribitol phosphate polysaccharide-tetanus protein (prp-t) conjugate vaccine when given to patients at 2, 4, and 6 months of age. | 1992 | 1731134 |
| cyclohexadienyl dehydratase from pseudomonas aeruginosa. molecular cloning of the gene and characterization of the gene product. | the gene encoding cyclohexadienyl dehydratase (denoted phec) was cloned from pseudomonas aeruginosa by functional complementation of a phea auxotroph of escherichia coli. the gene was highly expressed in e. coli due to the use of the high-copy number vector puc18. the p. aeruginosa cyclohexadienyl dehydratase expressed in e. coli was purified to electrophoretic homogeneity. the latter enzyme exhibited identical physical and biochemical properties as those obtained for cyclohexadienyl dehydratase ... | 1992 | 1733946 |
| dna sequences and characterization of dtxr alleles from corynebacterium diphtheriae pw8(-), 1030(-), and c7hm723(-). | the structural gene encoding dtxr, an iron-dependent diphtheria tox regulatory element, has recently been cloned and sequenced from the c7(-) strain of corynebacterium diphtheriae (j. m. boyd, m. oza, and j. r. murphy, proc. natl. acad. sci. usa 87:5972, 1990). we report here the molecular cloning, dna sequence analysis, and characterization of dtxr from the pw8(-), 1030(-), and c7hm723 strains of c. diphtheriae. while the sequence of dtxr from pw8(-) is identical to that of the c7(-) allele, th ... | 1992 | 1735717 |
| [pyomyositis]. | pyomyositis (pm) is characterized by bacterial infection and suppuration of striated muscle. it is very rarely encountered in temperate climates. only five cases have hitherto been reported in denmark. two cases of pm are presented. both of these demonstrate the difficulty of establishing early diagnosis and the severe complications of pm. | 1992 | 1738956 |
| screening tests for pathogenic corynebacteria. | to provide simple tests that would help in the identification of corynebacteria that produce diphtheria toxin. | 1992 | 1740514 |
| a c-terminal deletion in corynebacterium glutamicum homoserine dehydrogenase abolishes allosteric inhibition by l-threonine. | in escherichia coli, bacillus subtilis and corynebacterium glutamicum, homoserine dehydrogenase (hd), the enzyme after the branch point of the threonine/methionine and lysine biosynthetic pathways, is allosterically inhibited by l-threonine. to investigate the regulation of the c. glutamicum hd enzyme by l-threonine, the structural gene, hom, was mutated by uv irradiation of whole cells to obtain a deregulated allele, homdr. l-threonine inhibits the wild-type (wt) enzyme with a ki of 0.16 mm. th ... | 1991 | 1743520 |
| [a phase-i clinical trial of a new antitetanus/antidiphtheria vaccine for adults]. | | 1991 | 1725595 |
| antigen specificity of antibody responses to corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis. | constituents of corynebacterium pseudotuberculosis were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). analysis of sonicated whole bacterial cells and ether-extracted cells revealed more than 35 bands in silver-stained gels. sds-page analysis of concentrated culture filtrates with exotoxin activity demonstrated more than 15 bands. sera from sheep with c. pseudotuberculosis-induced disease of variable severity were used to probe immunoblots of electrophoresed e ... | 1991 | 1719692 |
| struvite stone formation by corynebacterium group f1: a case report. | struvite stones are caused by urea-splitting, usually gram-negative, organisms. a case of aggressive struvite stone production caused by corynebacterium group f1 is reported that responded to the appropriate antibiotic treatment. to our knowledge this organism has never been associated previously with struvite stone formation. | 1992 | 1729521 |
| histopathology of the early phase during experimental corynebacterium pseudotuberculosis infection in lambs. | histological responses during experimental corynebacterium pseudotuberculosis infection in lambs were investigated in parotid lymph nodes for ten days following inoculation. lambs were infected by the subcutaneous route into the right eyelid with a virulent strain of c. pseudotuberculosis. multiple microscopic acute abscesses, predominantly infiltrated with polymorphonuclear (pmn) leucocytes, were seen in the right parotid lymph node on the 1st day post-inoculation (pi). this massive pmn infiltr ... | 1991 | 1746152 |
| arcanobacterium haemolyticum brain abscess in a child (a case report). | | 1991 | 1752643 |
| mastitis in beef cows and its effects on calf weight gain. | quarter milk samples from 51 purebred (angus, polled hereford, and simmental) and 69 crossbred (angus x simmental x charolais three-way cross) beef cows were collected aseptically at three times during lactation to determine the prevalence of intramammary infection, milk somatic cell counts (scc), and effects of infection on calf weight gain. quarter infection prevalence was 13.1, 14.9, and 27.5% in early, mid, and late lactation; corresponding cow infection prevalence was 25.8, 29.2, and 54.4%. ... | 1991 | 1752802 |
| complementary imaging modalities in the diagnosis of a mycotic aneurysm. | | 1991 | 1752105 |
| inhibition of immune reactions in vivo by liposome associated transforming growth factor (tgf) type beta 1. | in view of its potent inhibitory capacity on immune cells in culture, we wished to determine the ability of transforming growth factor (tgf) beta 1 to down-regulate immune responses in vivo. preliminary experiments suggested that, at the doses used, systemic injection of soluble tgf beta 1 could not affect bacterial-induced spleen enlargement in mice. therefore, we sought to utilize a physiochemical property of this molecule, namely its high pi, to determine possible association between the liga ... | 1991 | 1747960 |