Publications

tuberculosis-like pneumonias by the aerobic actinomycetes rhodococcus, tsukamurella and gordonia.	the order actinomycetales includes phylogenetically diverse but morphologically similar aerobic and anaerobic organisms, exhibiting filamentous branching structures which fragment into rods or coccoid forms. lung pathogens of the order comprise mycobacterium, nocardia, corynebacterium, actinomyces, kytococcus, rothia, williamsia, as well as gordonia, tsukamurella and rhodococcus. particularly, members of the last three genera are uncommon aerobic agents of lung cavitations and tuberculosis(tb)-l ...	2011	22192786
Solution NMR structures reveal a distinct architecture and provide first structures for protein domain family PF04536.	The protein family (Pfam) PF04536 is a broadly conserved domain family of unknown function (DUF477), with more than 1,350 members in prokaryotic and eukaryotic proteins. High-quality NMR structures of the N-terminal domain comprising residues 41-180 of the 684-residue protein CG2496 from Corynebacterium glutamicum and the N-terminal domain comprising residues 35-182 of the 435-residue protein PG0361 from Porphyromonas gingivalis both exhibit an a/ß fold comprised of a four-stranded ß-sheet, thre ...	2011	22198206
Enzymatic synthesis of S-adenosylhomocysteine: immobilization of recombinant S-adenosylhomocysteine hydrolase from Corynebacterium glutamicum (ATCC 13032).	Recombinant S-adenosylhomocysteine hydrolase from Corynebacterium glutamicum (CgSAHase) was covalently bound to Eupergit® C. The maximum yield of bound protein was 91% and the catalytic efficiency was 96.9%. When the kinetic results for the immobilized enzyme were compared with those for the soluble enzyme, no decrease in the catalytic efficiency of the former was detected. Both soluble and immobilized enzymes showed similar optimum pH and temperature ranges. The reuse of immobilized CgSAHase ca ...	2011	22202964
Backbone and side chain (1)H, (13)C and (15)N resonance assignments of the catalytic domain of diphtheria toxin.	Diphtheria is a serious upper respiratory tract disease caused by the diphtheria toxin (DT) secreted from the bacteria Corynebacterium diphtheriae. Vaccination is the best way to protect against this infectious disease. Diphtheria vaccines are prepared by isolating, purifying and chemically deactivating DT. Although toxoids have been used for decades in immunization, there is still little understanding at the molecular level of the process of toxoid preparation, and how chemical treatment enhanc ...	2011	22205447
engineering corynebacterium glutamicum for the production of pyruvate.	a corynebacterium glutamicum strain with inactivated pyruvate dehydrogenase complex and a deletion of the gene encoding the pyruvate:quinone oxidoreductase produces about 19 mm l: -valine, 28 mm l: -alanine and about 55 mm pyruvate from 150 mm glucose. based on this double mutant c. glutamicum △acee △pqo, we engineered c. glutamicum for efficient production of pyruvate from glucose by additional deletion of the ldha gene encoding nad(+)-dependent l: -lactate dehydrogenase (ldha) and introduction ...	2012	22228312
corynebacterium diphtheriae 67-72p hemagglutinin, characterized as the protein dip0733, contributes to invasion and induction of apoptosis in hep-2 cells.	although corynebacterium diphtheriae has been classically described as an exclusively extracellular pathogen, there is growing evidence that it may be internalized by epithelial cells. the aim of the present report was to investigate the nature and involvement of the surface-exposed non-fimbrial 67-72 kda proteins (67-72p), previously characterized as adhesin/hemagglutinin, in c. diphtheriae internalization by hep-2 cells. transmission electron microscopy and bacterial internalization inhibition ...	2012	22239957
isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, bombyx mori, reared on different forage.	abstract the silkworm, bombyx mori l. (lepidoptera: bombycidae), an oligophagous insect that mainly feeds on mulberry leaves, is susceptible to entomopathogen infection when reared with tricuspid cudrania leaves. a total of 56 dominant bacterial strains, classified into 12 phylotypes based on bacteriological properties and analysis of 16s rrna genes, were isolated from the intestine of the fourth and fifth instar silkworm larvae. ten and seven phylotypes exist in the intestine of the silkworm ...	2011	22243438
genome sequence of corynebacterium casei ucma 3821, isolated from a smear-ripened cheese.	corynebacterium casei is one of the most prevalent species present on the surfaces of smear-ripened cheeses, where it contributes to the production of the desired organoleptic properties. here, we report the draft genome sequence of corynebacterium casei ucma 3821 to provide insights into its physiology.	2012	22247534
genome sequence of corynebacterium glutamicum atcc 14067, which provides insight into amino acid biosynthesis in coryneform bacteria.	we report the genome sequence of corynebacterium glutamicum atcc 14067 (once named brevibacterium flavum), which is useful for taxonomy research and further molecular breeding in amino acid production. preliminary comparison with those of the reported coryneform strains revealed some notable differences that might be related to the difficulties in molecular manipulation.	2012	22247536
[effect of gamma-glutamyl kinase gene knock-out on metabolism in l-arginine-producing strain corynebacterium crenatum 8-193].	in order to optimize precursor supply for l-arginine biosynthesis, we constructed a corynebacterium crenatum 8-193 mutant with gamma-glutamyl kinase gene (prob) in-frame deletion. the effects of prob knock-out on physiological characteristics of the mutant were investigated.	2011	22260045
regulation of the malic enzyme gene male by the transcriptional regulator malr in corynebacterium glutamicum.	corynebacterium glutamicum is a gram-positive nonpathogenic bacterium that is used for the biotechnological production of amino acids. here, we investigated the transcriptional control of the male gene encoding malic enzyme (male) in c. glutamicum atcc13032, which is known to involve the nitrogen regulator amtr. gel shift experiments using purified regulators rama and ramb revealed binding of these regulators to the male promoter. in dna-affinity purification experiments a hitherto uncharacteriz ...	2012	22261175
culture-independent exploration of the teat apex microbiota of dairy cows reveals a wide bacterial species diversity.	due to their close proximity to the mammary gland tissue, the bacterial communities lining the teat apex of the udders from lactating cows influence udder health. denaturing gradient gel electrophoresis of the amplified v3 variable region of the 16s rrna gene was used as a culture-independent method to reveal the bacterial composition of 48 samples originating from the teat apices of twelve friesian-holstein dairy cows suffering from clinical mastitis in one quarter. the microbiota belonged to f ...	2011	22266158