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strains of internal biofilm in aerobic granular membrane bioreactors.this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ...201020306189
arthrobacter sp. strain ku001 isolated from a thai soil degrades atrazine in the presence of inorganic nitrogen sources.an atrazine degrading bacterium, strain ku001, was obtained from a sugarcane field at the cane and sugar research and development center at the kasetsart university, kamphaeng saen campus, thailand. strain ku001 had a rod-to-coccus morphological cycle during growth. biolog carbon source analysis indicated that the isolated bacterium was arthrobacter histidinolovorans. sequence analysis of pcr product indicated the 16s rrna gene in strain ku001 was 99% identical to the same region in arthrobacter ...201020372034
cg2091 encodes a polyphosphate/atp-dependent glucokinase of corynebacterium glutamicum.the corynebacterium glutamicum gene cg2091 is encoding a polyphosphate (polyp)/atp-dependent glucokinase (ppgk). previous work demonstrated the association of ppgk to polyp granules. the deduced amino acid sequence of ppgk shows 45% sequence identity to polyp/atp glucomannokinase of arthrobacter sp. strain km and 50% sequence identity to polyp glucokinase of mycobacterium tuberculosis h37rv. ppgk from c. glutamicum was purified from recombinant escherichia coli. polyp was highly preferred over a ...201020379711
comparative study of properties of immobilized lipase onto glutaraldehyde-activated amino-silica gel via different methods.the enzyme-aggregate coating method was performed to immobilize arthrobacter sp. lipase in order to achieve better catalytic properties comparable to the conventional covalent attachment and covalent attachment plus cross-linking. the glutaraldehyde-activated amino-silica gel which was synthesized by sol-gel technique was used as the support, and the catalytic characteristics of the lipase preparations were tested in the asymmetric acylation of 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-o ...201020399626
homology modeling and docking study of xanthine oxidase of arthrobacter sp. xl26.hyperuricemia is a condition of defective purine metabolism characterized with elevated serum uric acid (ua) level that further leads to gout and gouty nephrolithiasis disorders. gout is a world wide distributed rheumatic disease comprises 1% of the total population and still is in increasing state. one of the factors contributing to overproduction of ua is the hydroxylation of xanthine catalyzed by xanthine oxidase (xo). in the present study, 3d modeling of arthrobacter sp. xl26 (xodb) protein ...201020403378
weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution.several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ...201020525872
influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments.because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ...201020553043
high-density polyethylene (hdpe)-degrading potential bacteria from marine ecosystem of gulf of mannar, india.assessment of high-density polyethylene (hdpe)-degrading bacteria isolated from plastic waste dumpsites of gulf of mannar.201020586938
study on the removal of indoor vocs using biotechnology.with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ...201020598440
soil bacterial communities in constructed wetlands treated with swine wastewater using pcr-dgge technique.marsh-pond-marsh (mpm) constructed wetlands were designed for the treatment of swine wastewater. the goal of this study was to characterize bacterial communities in these wetlands and determine the nutrient removal from influent to effluent. surface soil samples were collected and analyzed by culture-dependent and culture-independent techniques. the results showed that the bacterial colony forming units (cfu) and the average concentrations of total nitrogen, nh(4)(+), total phosphorous (tp) and ...201019822421
effects of bacterial treatment at early stages of atlantic cod (gadus morhua l.) on larval survival and development.to assess the effects of bacterial treatment at the earliest stages of cod rearing on the microbial load, larval development and performance, testing three bacterial strains (carnobacterium divergens v41, arthrobacter sp. and enterococcus sp.) in vivo that were previously shown to have inhibitory potential towards fish pathogens in vitro.201019664064
air-cathode microbial fuel cell array: a device for identifying and characterizing electrochemically active microbes.microbial fuel cells (mfcs) have generated excitement in environmental and bioenergy communities due to their potential for coupling wastewater treatment with energy generation and powering diverse devices. the pursuit of strategies such as improving microbial cultivation practices and optimizing mfc devices has increased power generating capacities of mfcs. however, surprisingly few microbial species with electrochemical activity in mfcs have been identified because current devices do not suppo ...201120655725
isolation and characterization of an arthrobacter sp. strain hb-5 that transforms atrazine.a bacterial strain (hb-5) capable of utilizing atrazine as sole carbon and nitrogen source for growth was isolated from an industrial wastewater sample by enrichment culture. the isolate was identified as arthrobacter sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. the strain exhibited faster atrazine degradation rates in atrazine-containing mineral media than the well-characterized atrazine-degrading bacteria pseudomonas sp. adp. ...201120686824
genomic heterogeneity within conservedmetabolic pathways of arthrobacter species - a bioinformatic approach.a comparative genomic analysis of three species of the soil bacterium arthrobacter was undertaken with specific emphasis on genes involved in important and core energy metabolism pathways like glycolysis and amino acid metabolism. during the course of this study, it was revealed that codon bias of a particular species, namely arthrobacter aurescens tc1, is significantly lower than that of the other two species a. chlorophenolicus a6 and arthrobacter sp. fb24. the codon bias was also found to be ...201121423891
molecular characterization of cold-inducible beta-galactosidase from arthrobacter sp. on14 isolated from antarctica.a psychrotrophic bacterium, arthrobacter sp. on14, isolated from antarctica, was shown to exhibit a high beta-galactosidase activity at a low temperature. a genomic library of on14 was constructed and screened for beta-galactosidase genes on functional plates containing 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (x-gal) as the substrate. two different beta-galactosidase genes, named as gala, galb, were found in on14. computational analyses of the genes revealed that the encoded protein ...201121464592
enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from arthrobacter sp.in human serum, as for phospholipids not containing choline, phosphatidylethanolamine (pe) exists approximately 5% in a whole phospholipid. pe is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis and cell signaling. however, it could not measure pe with other phospholipids due to a lack of choline in them.201121549106
screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey.several microorganisms were isolated from soil/sediment samples of antarctic peninsula. the enrichment technique using (rs)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. we also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (rs)-1-(phenyl)ethanol as substrates. among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze th ...201121673897
phylogenetic perspectives of nitrogen-fixing actinobacteria.it was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus frankia. recently, the number of nitrogen fixation (nifh) genes identified in other non-frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. during the last decade, mycobacterium flavum, corynebacterium autotrophicum and a fluorescent arthr ...201121779790
a multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion.this study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (fragaria ananassa) fruit. a total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-pcr banding pattern and biochemical features. phylogenetic analysis of the 16s rrna gene sequences of 45 representatives showed that the isolates belonged to t ...201121837472
biodegradation kinetics of 4-fluorocinnamic acid by a consortium of arthrobacter and ralstonia strains.arthrobacter sp. strain g1 is able to grow on 4-fluorocinnamic acid (4-fca) as sole carbon source. the organism converts 4-fca into 4-fluorobenzoic acid (4-fba) and utilizes the two-carbon side-chain for growth with some formation of 4-fluoroacetophenone as a dead-end side product. we also have isolated ralstonia sp. strain h1, an organism that degrades 4-fba. a consortium of strains g1 and h1 degraded 4-fca with monod kinetics during growth in batch and continuous cultures. specific growth rate ...201121728015
an isofenphos-methyl hydrolase (imh) capable of hydrolyzing the p-o-z moiety of organophosphorus pesticides containing an aryl or heterocyclic group.organophosphorus pesticide (op) hydrolases play key roles in the degradation and decontamination of agricultural and household ops and in the detoxification of chemical warfare agents. in this study, an isofenphos-methyl hydrolase gene (imh) was cloned from the isocarbophos-degrading strain of arthrobacter sp. scl-2 using the polymerase chain reaction method. isofenphos-methyl hydrolase (imh) showed 98% sequence identity with the isofenphos hydrolase from arthrobacter sp. strain b-5. imh was hig ...201122120622
production of xylanase from arthrobacter sp. mtcc 6915 using saw dust as substrate under solid state fermentation.saw dust was used as substrate for xylanase production from arthrobacter sp. mtcc 6915. the study of period of incubation, temperature, ph, carbon, and nitrogen sources for xylanase production was optimized. xylanase production was found to be optimum at an incubation period of 96 hrs (117.0 u/ml), temperature 30°c (105.0 u/ml), and ph 9.0 (102.9 u/ml). the results showed that the xylanase production was found to be higher in the presence of carboxymethylcellulose (176.4 u/ml) and dextrose (126. ...201122013512
complete detoxification of tris(1,3-dichloro-2-propyl) phosphate by mixed two bacteria, sphingobium sp. strain tcm1 and arthrobacter sp. strain py1.tris(1,3-dichloro-2-propyl) phosphate (tdcpp), a flame retardant, is regarded as a potentially toxic and persistent environmental contaminant. we previously isolated a tdcpp-degrading bacterium, sphingobium sp. strain tcm1, which, however, produced a toxic metabolite: 1,3-dichloro-2-propanol (1,3-dcp). this study was undertaken to develop a technique for complete tdcpp detoxification using strain tcm1 with a 1,3-dcp-degrading bacterium, arthrobacter sp. strain py1. for efficient detoxification, ...201121956155
a novel transaminase, (r)-amine:pyruvate aminotransferase, from arthrobacter sp. knk168 (ferm bp-5228): purification, characterization, and gene cloning.a novel (r)-amine transaminase, which catalyzed (r)-enantioselective transamination of chiral amine, was purified to homogeneity from arthrobacter sp. knk168 (ferm bp-5228). the molecular mass of the enzyme was estimated to be 148 kda by gel filtration and 37 kda by sodium dodecyl sulfate polyacrylamide gel electrophoresis, suggesting a homotetrameric structure. the enzyme catalyzed transamination between amines and pyruvate stereo-specifically. the reaction on 1-methylbenzylamine was (r)-enanti ...201122002066
the three-dimensional structure of nylon hydrolase and the mechanism of nylon-6 hydrolysis.we performed x-ray crystallographic analyses of the 6-aminohexanoate oligomer hydrolase (nylc) from agromyces sp. at 2.0 å-resolution. this enzyme is a member of the n-terminal nucleophile (n-tn) hydrolase superfamily that is responsible for the degradation of the nylon-6 industry byproduct. we observed four identical heterodimers (27kda+9kda), which resulted from the autoprocessing of the precursor protein (36kda) and which constitute the doughnut-shaped quaternary structure. the catalytic resi ...201122187439
[High throughput screening atrazine chlorohydrolase mutants with enhanced activity through Haematococcus pluvialis expression system].Developing a high-throughput screening method is of great importance for directed evolution of atrazine chlorohydrolase. A mutagenesis library of atzA from Pseudomonas sp. ADP and Arthrobacter sp. AD1 was constructed using error-prone PCR and DNA shuffling. Candidate mutants were screened through Haematococcus pluvialis expression system, using atrazine as selection pressure. Sequence analysis showed that mutations in the obtained 12 mutants with enhanced activity were all point-substitutions an ...201121847998
Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy.Halohydrin dehalogenases are attractive biocatalysts in producing a series of important chiral building blocks. Recombinant expression of halohydrin dehalogenase from Arthrobacter sp. AD2 (HheA) in Escherichia coli using T7 promoter-based pGEF(+) system revealed much lower expression level than that of the well-studied halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC). In this study, we changed the codon usage in the 5'-end of hheA gene to improve the expression yield of HheA. Ou ...201122112566
construction of escherichia coli-arthrobacter-rhodococcus shuttle vectors based on a cryptic plasmid from arthrobacter rhombi and investigation of their application for functional screening.a cryptic plasmid from arthrobacter rhombi prh1, designated as pprh, was sequenced and characterized. it was 5000 bp in length with a g+c content of 66 mol%. the plasmid pprh was predicted to encode six putative open reading frames (orfs), in which orf2 and orf3 formed the minimal replicon of plasmid pprh and shared 55-61% and 60-69% homology, respectively, with the repa and repb proteins of reported rhodococcal plasmids. sequence analysis revealed a typical cole2-type ori located 45 bp upstream ...201122098420
[study on 2-methylpyridine initial catabolic pathways in arthrobacter sp. km-2mp strain]. 201121861369
impact of pgpr inoculation on growth and antioxidant status of wheat under saline conditions.two plant growth-promoting rhizobacterial (pgpr) strains, bacillus subtilis su47 and arthrobacter sp. su18, were found to tolerate 8% nacl. wheat co-inoculated with these two pgpr strains, and grown under different salinity regimes (2-6 ds m(-1) ), showed an increase in dry biomass, total soluble sugars and proline content. wheat sodium content was reduced under co-inoculated conditions but not after single inoculation with either strain or in the control. the activity of antioxidant enzymes in ...201122136617
pathway for degradation of 2-chloro-4-nitrophenol in arthrobacter sp. sjcon.degradation of 2-chloro-4-nitrophenol (2c4np) was studied by arthrobacter sp. sjcon, isolated from the soil of a pesticide contaminated site. this strain utilized 2c4np as sole source of carbon and energy and degraded 2c4np with stoichiometric release of nitrite and chloride ions. a metabolite was detected during the study of 2c4np degradation and identified as chlorohydroquinone (chq) by thin layer chromatography (tlc), high performance liquid chromatography (hplc), and gas chromatography-mass ...201121960016
production of cyclic adenosine monophosphate by arthrobacter sp. a302 using fed-batch fermentation with ph-shift control.the production of cyclic adenosine monophosphate (camp) by arthrobacter sp. a302 was studied in a 5 l stirred tank fermentor under a range of ph values (6.5-8.0) and glucose feeding rates. in batch fermentation under a controlled ph, the optimum ph for cell growth was 7.5 with dry cell density (x) of 11.43 g l, and the optimum ph for camp accumulation was 7.0 with camp concentration of 7.41 g l. in order to achieve the high x and camp yield simultaneously, a ph-shift control strategy was propose ...201122806787
study on the flocculability of the arthrobacter sp., an actinomycete resuscitated from the vbnc state.a bioflocculant with high flocculating activity, lc13-sf, produced by strain lc13(t) which was in a viable but nonculturable (vbnc) state, and which was woken up by rpf (resuscitation promoting factor), was systematically investigated with regard to its fermentation conditions and flocculating activity. the key parameters influencing the bioflocculant lc13-sf were investigated through measuring the optical density at 660 (od(660)) of the fermentation liquid and the optical density at 550 (od(550 ...201122806783
chemotaxis to atrazine and detection of a xenobiotic catabolic plasmid in arthrobacter sp. dns10.a plasmid named pdns10 was detected from an atrazine-degrading strain arthrobacter sp. dns10 which has been isolated previously in our laboratory.201122351258
[effect of cellulose-decomposing strain on microbial community of cow manure compost].taking the cow dung and straw as composting raw materials, effect of cellulose-decomposing strain on microbial community of cow manure compost was investigated with the traditional culture method and pcr-dgge technique. the results showed that the microbiological inocula showed a more rapid rate of temperature elevation at the start of composting and prolonged the time of high-temperature process and increased the number of microbial. the dgge map of cellulose-decomposing strain compost was diff ...201122279926
cloning of inulin fructotransferase (dfa iii-producing) gene from arthrobacter sp. l68-1.a gene of inulin fructotransferase (dfa iii-producing) [ec 2.4.1.93] from arthrobacter sp. l68-1 was cloned and the nucleotide was sequenced. the gene encoded a signal peptide (32 amino acid residues) for a secretion, and the mature enzyme protein was estimated to be consisted with 410 amino acid residues. the molecular mass of the native enzyme was calculated as 43.7kda by the sequence data. the deduced amino acid sequence of the enzyme had 79.0% homology with that of the arthrobacter globiform ...201223499085
the paax-type repressor meqr2 of arthrobacter sp. strain rue61a, involved in the regulation of quinaldine catabolism, binds to its own promoter and to catabolic promoters and specifically responds to anthraniloyl coenzyme a.the genes coding for quinaldine catabolism in arthrobacter sp. strain rue61a are clustered on the linear plasmid pal1 in two upper pathway operons (meqabc and meqdef) coding for quinaldine conversion to anthranilate and a lower pathway operon encoding anthranilate degradation via coenzyme a (coa) thioester intermediates. the meqr2 gene, located immediately downstream of the catabolic genes, codes for a paax-type transcriptional repressor. meqr2, purified as recombinant fusion protein, forms a di ...201223275246
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