Publications

cultivation-independent examination of horizontal transfer and host range of an incp-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere.	the host range and transfer frequency of an incp-1 plasmid (pkjk10) among indigenous bacteria in the barley rhizosphere was investigated. a new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16s rrna gene classification was used. indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16s rrna genes from the sorted cells. the host range of th ...	2006	17021220
protein engineering of a cold-active beta-galactosidase from arthrobacter sp. sb to increase lactose hydrolysis reveals new sites affecting low temperature activity.	we examined variants of an especially cold-active beta-galactosidase (bgas) to better understand features affecting enzyme activity at temperature extremes. we targeted locations corresponding to a region in the lacz enzyme previously shown to increase activity and decrease thermostability. changes in this region of bgas consistently caused the elimination or reduction of activity. a gene (bgas3) encoding a loss of function variant was subjected to random mutagenesis to restore activity and disc ...	2006	16736094
contribution of ethylamine degrading bacteria to atrazine degradation in soils.	bacterial communities that cooperatively degrade atrazine commonly consist of diverse species in which the genes for atrazine dechlorination and dealkylation are variously distributed among different species. normally, the first step in degradation of atrazine involves dechlorination mediated by atza, followed by stepwise dealkylation to yield either n-ethylammelide or n-isopropylammelide. as the liberated alkylamine moieties are constituents of many organic molecules other than atrazine, it is ...	2006	17064268
crystallization and x-ray diffraction analysis of 6-aminohexanoate-cyclic-dimer hydrolase from arthrobacter sp. ki72.	6-aminohexanoate-cyclic-dimer hydrolase (ei) from arthrobacter sp. ki72 was expressed in escherichia coli and purified by anion-exchange chromatography. ei was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as precipitant in imidazole buffer ph 8.0. the crystal is hexagonal, with unit-cell parameters a = b = 130.75, c = 58.23 a. diffraction data were collected from native and mercury(ii) dichloride-derivative crystals to resolutions of 1.90 and 2.06 a, respectively.	2006	17142898
bacterial endophytes contribute to abiotic stress adaptation in pepper plants (capsicum annuum l.).	endophytes are nonpathogenic plant-associated bacteria that can play an important role in plant vitality and may confer resistance to abiotic or biotic stress. the effects of 5 endophytic bacterial strains isolated from pepper plants showing 1-aminocyclopropane-1-carboxylate deaminase activity were studied in sweet pepper under in vitro conditions. four of the strains tested showed production of indole acetic acid. plant growth, osmotic potential, free proline content, and gene expression were m ...	2007	18026213
cloning and characterization of the gene encoding endo-beta-1,3-glucanase from arthrobacter sp. nhb-10.	the glua gene, encoding an endo-beta-1,3-glucanase from arthrobacter sp. (strain nhb-10), was cloned and analyzed. the deduced endo-beta-1,3-glucanase amino acid sequence was 750 amino acids long and contained a 42 amino acid signal peptide with a mature protein of 708 amino acids. there was no similarity to known endo-beta-1,3-glucanases, but glua was partially similar to two fungal exo-beta-1,3-glucanases in glycoside hydrolase (gh) family 55. of five possible residues for catalysis and two mo ...	2007	17587693
cloning of a gene cluster involved in the catabolism of p-nitrophenol by arthrobacter sp. strain js443 and characterization of the p-nitrophenol monooxygenase.	the npd gene cluster, which encodes the enzymes of a p-nitrophenol catabolic pathway from arthrobacter sp. strain js443, was cloned and sequenced. three genes, npdb, npda1, and npda2, were independently expressed in escherichia coli in order to confirm the identities of their gene products. npda2 is a p-nitrophenol monooxygenase belonging to the two-component flavin-diffusible monooxygenase family of reduced flavin-dependent monooxygenases. npda1 is an nadh-dependent flavin reductase, and npdb i ...	2007	17720792
6-aminohexanoate oligomer hydrolases from the alkalophilic bacteria agromyces sp. strain ky5r and kocuria sp. strain ky2.	alkalophilic, nylon oligomer-degrading strains, agromyces sp. and kocuria sp., were isolated from the wastewater of a nylon-6 factory and from activated sludge from a sewage disposal plant. the 6-aminohexanoate oligomer hydrolases (nylc) from the alkalophilic strains had 95.8 to 98.6% similarity to the enzyme in neutrophilic arthrobacter sp. but had superior thermostability, activity under alkaline conditions, and affinity for nylon-related substrates, which would be advantageous for biotechnolo ...	2007	17827307
coryneform bacteria in semen of chronic prostatitis patients.	our aim was to compare the presence and species composition of coryneform bacteria in chronic prostatitis patients and controls. semen of 50 men with inflammatory prostatitis and 59 controls (without pelvic pain/discomfort complaints and leukocytospermia) was investigated. first-catch urine was additionally investigated in 36 men (30 with and 6 without prostatitis). coryneform bacteria were found in semen of 76% men with inflammatory prostatitis and 83% controls. more than half of the isolates w ...	2007	17163953
taxonomic identification, phenanthrene uptake activity, and membrane lipid alterations of the pah degrading arthrobacter sp. strain sphe3.	this report describes phenanthrene uptake as well as the effect of phenanthrene on the membrane phospholipid and fatty acid composition in a newly isolated bacterial strain, sphe3, that we taxonomically identified as arthrobacter sp. strain sphe3 is able to utilize phenanthrene as a carbon source at high rates and appears to internalize phenanthrene with two mechanisms: a passive diffusion when cells are grown on glucose, and an inducible active transport system when cells are grown on phenanthr ...	2007	17583808
stability of microbial communities in goat milk during a lactation year: molecular approaches.	the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...	2007	17604934
mineralization of diuron [3-(3,4-dichlorophenyl)-1, 1-dimethylurea] by co-immobilized arthrobacter sp. and delftia acidovorans.	mineralization of diuron has not been previously demonstrated despite the availability of some bacteria to degrade diuron into 3,4-dichloroaniline (3,4-dca) and others that can mineralize 3,4-dca. a bacterial co-culture of arthrobacter sp. n4 and delftia acidovorans w34, which respectively degraded diuron (20 mg l(-1)) to 3,4-dca and mineralized 3,4-dca, were able to mineralize diuron. total diuron mineralization (20 mg l(-1)) was achieved with free cells in co-culture. when the bacteria were im ...	2007	17333465
cloning of the arthrobacter sp. fg1 dehalogenase genes and construction of hybrid pathways in pseudomonas putida strains.	an arthrobacter strain, able to utilize 4-chlorobenzoic acid as the sole carbon and energy source, was isolated and characterized. the first step of the catabolic pathway was found to proceed via a hydrolytic dehalogenation that leads to the formation of 4-hydroxybenzoic acid. the dehalogenase encoding genes (fcb) were sequenced and found highly homologous to and organized as those of other 4-chlorobenzoic acid degrading arthrobacter strains. the fcb genes were cloned and successfully expressed ...	2007	17384944
the characteristics of rhizosphere microbes associated with plants in arsenic-contaminated soils from cattle dip sites.	soil microorganisms and plants were studied in samples of arsenic-contaminated soil from two cattle dip sites. the aim was to delineate the parameters that will determine the feasibility of future remediation by growing arsenic-accumulating plants, including the identity and characteristics of some rhizosphere soil microbes. the soil samples contained high total, but low soluble arsenic concentrations which, together with other properties, resembled the previously reported characteristics of dip ...	2007	17407787
[reaction of microorganisms to the digestive fluid of the earthworms].	the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ...	2007	17410875
[screening and function analysis of a cyclohexanone-degrading bacterium cn1 from deep sea sediment].	micrococcu luteus cn1 was found to be able to utilize cyclohexanone well from the strains originally isolated from pacific ocean sediment. the optimum conditions for its growth were determined as 25 degrees c -37 degrees c, ph 8, salinity 6%. it could survive in the medium with high concentration of cyclohexanone ( > 44% v/v), and grew most vigorously in medium with 16.7% (v/v) cyclohexanone. cn1 could transform cyclohexanol to cyclohexanone which could be further degraded and mineralized quickl ...	2007	18062257
amperometric biosensor for the determination of creatine.	an amperometric biosensor for the determination of creatine was developed. the carbon rod electrode surface was coated with sarcosine oxidase (sox) and creatine amidinohydrolase by cross-linking under glutaraldehyde vapour. the sox from arthrobacter sp. 1-1 n was purified and previously used for creation of a creatine biosensor. the natural sox electron acceptor, oxygen, was replaced by an [fe(cn)(6)](3-) /[ fe(cn)(6)](4-) redox mediating system, which allowed amperometric detection of an analyt ...	2007	17221239
solar-based detoxification of phenol and p-nitrophenol by sequential tio2 photocatalysis and photosynthetically aerated biological treatment.	simulated solar uv/tio(2) photocatalysis was efficient to detoxify a mixture of 100 mgphenoll(-1) and 50 mgp-nitrophenol (pnp) l(-1) and allow the subsequent biodegradation of the remaining pollutants and their photocatalytic products under photosynthetic aeration with chlorella vulgaris. photocatalytic degradation of phenol and pnp was well described by pseudo-first order kinetics (r(2)>0.98) with removal rate constants of 1.9x10(-4) and 2.8x10(-4)min(-1), respectively, when the pollutants were ...	2007	17350074
[isolation and degrading characters of chlorpyrifos degrading bacteria xz-3].	a strain of bacterium xz-3 capable of highly degrading chlorpyrifos was screened from the soil sample collected from a pesticide plant after taming and enrichment. based on analysis of phenotype, physiological and biochemical characters and 16s rdna, xz-3 was identified as arthrobacter sp.. the effects of carbon source, ph, temperature and chlorpyrifos concentration on degradation were determined. the growth of bacteria in culture media were measured by visible absorption spectrophotometry under ...	2007	18290445
aerobic uranium (vi) bioprecipitation by metal-resistant bacteria isolated from radionuclide- and metal-contaminated subsurface soils.	in this study, the immobilization of toxic uranium [u(vi)] mediated by the intrinsic phosphatase activities of naturally occurring bacteria isolated from contaminated subsurface soils was examined. the phosphatase phenotypes of strains belonging to the genera, arthrobacter, bacillus and rahnella, previously isolated from subsurface soils at the us department of energy's (doe) oak ridge field research center (orfrc), were determined. the orfrc represents a unique, extreme environment consisting o ...	2007	17991039
[study on the atrazine-degrading genes in arthrobacter sp. ag1].	atrazine could be used as the sole carbon, nitrogen and energy sources for growth by strain arthrobacter sp. ag1, and the atrazine-degrading genes of ag1 were found to be the combination of trzn, atzb and atzc. the atrazine chloride hydrolysase gene trzn was cloned by pcr amplification,whose sequence shared 99% identity with that of norcardioides sp. c190. two large plasmids were found in ag1,and trzn and atzb were confirmed to be localized on the larger plasmid pag1 by the method of southern hy ...	2007	18051853
application of thermoalkalophilic xylanase from arthrobacter sp. mtcc 5214 in biobleaching of kraft pulp.	a thermoalkalophilic and cellulase-free xylanase produced from arthrobacter sp. mtcc 5214 by solid-state fermentation using wheat bran as a carbon source was evaluated for prebleaching of kraft pulp. the uv absorption spectrum of the compounds released by enzyme treatment showed a characteristic peak at 280 nm, indicating the presence of lignin in the released colouring matter. enzymatic prebleaching of kraft pulp showed 20% reduction in kappa number of the pulp without much change in viscosity. ...	2007	16617015
degradation of pentachlorophenol by pure and mixed cultures in two different soils.	pentachlorophenol (pcp) is the second highest volume pesticide used in the united states. it is a mutagenic compound whose exposure poses significant health effects, one of the most desirable, environmentally friendly treatment methods is bioremediation. for soil-based contamination, the effectiveness of bioremediation will also be affected by the presence of an active indigenous population, sorption of the contaminant onto the soil, and environmental parameters.	2007	17668821
characterization of bacterial isolates from industrial wastewater according to probable modes of hexadecane uptake.	bacterial isolates from industrial wastewater were characterized according to probable modes of hexadecane uptake based on data for cell surface hydrophobicity, emulsifying activity, glycoside content and surface tension of cell-free culture medium. the results obtained suggested that both modes of biosurfactant-enhanced hexadecane uptake by bacterial strains take place, direct uptake and alkane transfer. the increase in cell surface hydrophobicity and glycoside production by the strains suggest ...	2008	16962302
complete degradation of butyl benzyl phthalate by a defined bacterial consortium: role of individual isolates in the assimilation pathway.	two bacterial strains, in consortium, were isolated by enrichment techniques from municipal waste-contaminated soil, which utilized butyl benzyl phthalate (bbp) as the sole carbon source. one of the isolates was identified as arthrobacter sp. strain wy and the other one as acinetobacter sp. strain fw based on the morphological, nutritional and biochemical characteristics and 16s rrna sequence analysis. various metabolites of bbp engendered by arthrobacter sp. strain wy were isolated and identifi ...	2008	17669462
continuous deodorization and bacterial community analysis of a biofilter treating nitrogen-containing gases from swine waste storage pits.	a biofilter inoculated with arthrobacter sp. was applied to the simultaneous elimination of trimethylamine (tma) and ammonia (nh3) from the exhaust air of swine waste storage pits. the results showed that the biofilter achieved average removal efficiencies of 96.8+/-2.5% and 97.2+/-2.3% for tma and nh3, respectively. a near-neutral ph (7.3-7.4) was maintained due to the accumulation of acid metabolites and the adsorption of alkaline nh3. low moisture demand, low pressure drop and high biofilm st ...	2008	17697773
biodegradation and bioremediation of endosulfan contaminated soil.	among the three mixed bacterial culture ae, be, and ce, developed by enrichment technique with endosulfan as sole carbon source, consortium ce was found to be the most efficient with 72% and 87% degradation of alpha-endosulfan and beta-endosulfan, respectively, in 20 days. in soil microcosm, consortium ae, be and ce degraded alpha-endosulfan by 57%, 88% and 91%, respectively, whereas beta-endosulfan was degraded by 4%, 60% and 67% after 30 days. ochrobacterum sp., arthrobacter sp., and burkholde ...	2008	17646098
enzymatic characterization of an amine oxidase from arthrobacter sp. used to measure phosphatidylethanolamine.	ethanolamine oxidase was screened with the aim of using it to establish a novel enzymatic phosphatidylethanolamine assay. ethanolamine oxidase activity was detected in the crude extract of arthrobacter sp., and the enzyme was purified more than 15-fold in three steps with a 54% yield. sds-page revealed the presence of only one band, which migrated, with an apparent molecular mass of 70 kda. biochemical characterization of the enzyme showed phenylethylamine to be the preferred substrate, with the ...	2008	18838796
mineralization of p-nitrophenol by a new isolate arthrobacter sp. y1.	arthrobacter sp. y1, capable of metabolizing p-nitrophenol (pnp) as the sole carbon, nitrogen and energy source was isolated from activated sludge. the bacterium could tolerate concentrations of pnp up to 600 mg l(- 1), and degradation of pnp was achieved within 120 h of incubation. pnp and its metabolites were analyzed by high performance liquid chromatography (hplc). the metabolite formed indicated that the organism followed the 4-nitrocathechol (4-nc) pathway for metabolism of this compound. ...	2008	18941993
isolation and characterization of atrazine-degrading arthrobacter sp. ad26 and use of this strain in bioremediation of contaminated soil.	a bacterial strain (ad26) capable of utilizing atrazine as a sole nitrogen source for growth was isolated from an industrial wastewater sample by enrichment culture. the 16s rrna gene sequencing identified ad26 as an arthrobacter sp. pcr assays indicated that ad26 contained atrazine-degrading genes trzn and atzbc. the trzn gene of ad26 only differs from the trzn of arthrobacter aurescens tc1 by one base (a-->t at 907) and one amino acid (met-->leu at 303). the specific activity of trzn of ad26 i ...	2008	19143347
fluorinated phenylcyclopropylamines. part 6: effects of electron withdrawing or donating aryl substituents on the inhibition of tyramine oxidase from arthrobacter sp. by diastereomeric 2-aryl-2-fluoro-cyclopropylamines.	diastereomeric arylcyclopropylamines substituted with fluorine in the 2-position and with electron donating or electron withdrawing groups at the aromatic ring were evaluated as inhibitors of microbial tyramine oxidase. the trans-isomers were consistently more potent inhibitors of the enzyme than the cis-isomers. electron donating substituents increased the potency of tyramine oxidase inhibition, while electron withdrawing substituents decreased the activity. the results obtained are discussed i ...	2008	19727324
metabolic cooperation of gordonia sp. strain mtcc 4818 and arthrobacter sp. strain wy in the utilization of butyl benzyl phthalate: effect of a novel co-culture in the degradation of a mixture of phthalates.	degradation of butyl benzyl phthalate (bbp) by a co-culture of gordonia sp. strain mtcc 4818 and arthrobacter sp. strain wy was investigated. in the degradation of bbp by the co-culture, the limitations of the individual species in metabolizing bbp were overcome, leading to the development of a consortium capable of complete utilization of this ester. in the degradation of bbp by the co-culture, the presence of multiple esterases was demonstrated in both species by activity staining of non-denat ...	2008	18957587
effect of immobilization on kinetic and thermodynamic characteristics of sulfide oxidase from arthrobacter species.	in order to determine the impact of immobilization on biocatalytic efficacy of sulfide oxidase, the kinetic and thermodynamic properties of native and deae-cellulose immobilized sulfide oxidase from arthrobacter species fr-3 were evaluated. immobilization increased the catalytic efficiency of sulfide oxidase by producing a lower michaelis-menten constant (km) and a higher rate of catalysis (vmax) at different temperatures. the first-order kinetic analysis of thermal denaturation demonstrated tha ...	2008	18080911
biofiltration of trimethylamine, dimethylamine, and methylamine by immobilized paracoccus sp. cp2 and arthrobacter sp. cp1.	a biofilter using granular activated carbon with immobilized paracoccus sp. cp2 was applied to the elimination of 10-250 ppm of trimethylamine (tma), dimethylamine (dma), and methylamine (ma). the results indicated that the system effectively treated ma (>93%), dma (>90%), and tma (>85%) under high loading conditions, and the maximum degradation rates were 1.4, 1.2, and 0.9g-nkg(-1) gac d(-1). among the three different amines treated, tma was the most difficult to degrade and resulted in ammonia ...	2008	18331754
multi-compound electrophoretic assays for tyramine oxidase with a uv area detector imaging multiple windows on a looped capillary.	active pixel sensor uv area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (emma) assay for substrate specificity of tyramine oxidase (arthrobacter sp.). use of the uv area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. continuo ...	2008	18589431
selection and identification of bacterial strains with methyl-tert-butyl ether, ethyl-tert-butyl ether, and tert-amyl methyl ether degrading capacities.	nine bacterial strains isolated from two hydrocarbon-contaminated soils were selected because of their capacity for growth in culture media amended with 200 mg/l of one of the following gasoline oxygenates: methyl-tert-butyl ether (mtbe), ethyl-tert-butyl ether (etbe), and tert-amyl methyl ether (tame). these strains were identified by amplification of their 16s rrna gene, using fdl and rd1 primers, and were tested for their capacity to grow and biotransform these oxygenates in both mineral and ...	2008	18522454
novel chromium tolerant microorganisms: isolation, characterization and their biosorption capacity.	effluent from tannery, electroplating and electronic industries contain chromium which is highly toxic. this paper reports isolation of chromium tolerant microorganisms from solid waste as well as liquid effluent of an electroplating industry. nine isolates were obtained that can tolerate chromium concentration up to 700mg/l. they reach their stationary phase within 8-12h and can biosorb 95% of initial 200mg/l concentration of chromium within 4-10h. fourier transform infra red analysis of the bi ...	2008	18272220
effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation.	spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ...	2008	18083857
degradation of 4-fluorophenol by arthrobacter sp. strain if1.	a gram-positive bacterial strain capable of aerobic biodegradation of 4-fluorophenol (4-fp) as the sole source of carbon and energy was isolated by selective enrichment from soil samples collected near an industrial site. the organism, designated strain if1, was identified as a member of the genus arthrobacter on the basis of 16s ribosomal rna gene sequence analysis. arthrobacter strain if1 was able to mineralize 4-fp up to concentrations of 5 mm in batch culture. stoichiometric release of fluor ...	2008	18228015
catalytic mechanism of inulinase from arthrobacter sp. s37.	detailed catalytic roles of the conserved glu323, asp460, and glu519 of arthrobacter sp. s37 inulinase (enia), a member of the glycoside hydrolase family 32, were investigated by site-directed mutagenesis and ph-dependence studies of the enzyme efficiency and homology modeling were carried out for enia and for d460e mutant. the enzyme efficiency (k(cat)/k(m)) of the e323a and e519a mutants was significantly lower than that of the wild-type due to a substantial decrease in k(cat), but not due to ...	2008	18395004
study of cadmium, zinc and lead biosorption by orange wastes using the subsequent addition method.	the biosorption of several metals (cd2+, zn2+ and pb2+) by orange wastes has been investigated in binary systems. multicomponent sorption isotherms were obtained using an original procedure, similar to that proposed by pagnanelli et al. [pagnanelli, f., petrangeli, m.p., toro, l., trifoni, m., veglio, f., 2001a. biosorption of metal ions on arthrobacter sp.: biomass characterization and biosorption modelling. environ. sci. technol. 34, 2773-2778] for monoelement systems, known as subsequent addi ...	2008	18440805
[restriction endonuclease asi256i recognizing the nucleotide sequence 5'-gatc-3'].	a strain producing a restriction endonuclease was isolated from soil samples and identified as the arthrobacter sp. strain ck256. the enzyme produced by this strain was termed asi2561. the isolation procedure for this enzyme was described, and the optimal conditions for its function were determined. it was shown that the restriction endonuclease asi256i is a true isoschizomer of mboi, it has a temperature optimum of 6 degrees c, and can be used in molecular-biological and genetic-engineering stu ...	2008	18491594
the screening, characterization, and use of omega-laurolactam hydrolase: a new enzymatic synthesis of 12-aminolauric acid.	several omega-laurolactam degrading microorganisms were isolated from soil samples. these strains were capable of growing in a medium containing omega-laurolactam as sole source of carbon and nitrogen. among them, five strains (t7, t31, u124, u224, and u238) were identified as cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, rhodococcus sp. u224, and sphingomonas sp. u238, respectively. the omega-laurolactam hydrolyzing enzyme from rhodococcus sp. u224 was purified to homogeneity, a ...	2008	18685217
genome sequence of the fish pathogen renibacterium salmoninarum suggests reductive evolution away from an environmental arthrobacter ancestor.	renibacterium salmoninarum is the causative agent of bacterial kidney disease and a significant threat to healthy and sustainable production of salmonid fish worldwide. this pathogen is difficult to culture in vitro, genetic manipulation is challenging, and current therapies and preventative strategies are only marginally effective in preventing disease. the complete genome of r. salmoninarum atcc 33209 was sequenced and shown to be a 3,155,250-bp circular chromosome that is predicted to contain ...	2008	18723615
application of 16s rdna-dgge and plate culture to characterization of bacterial communities associated with the sawfly, acantholyda erythrocephala (hymenoptera, pamphiliidae).	culture-based analysis was employed in parallel with pcr amplification of 16s rdna, coupled with denaturing gradient gel electrophoresis (dgge), to profile bacterial species associated with different developmental stages of the pine false webworm (pfw), acantholyda erythrocephala, a sawfly pest responsible for incidents of severe defoliation in commercially important tree plantations in north america. culture-based analysis revealed that pseudomonas spp. along with bacillus sphaericus and arthro ...	2008	18769850
structure and denaturation of 4-chlorobenzoyl coenzyme a dehalogenase from arthrobacter sp. strain tm-1.	the secondary structure of the trimeric protein 4-chlorobenzoyl coenzyme a dehalogenase from arthrobacter sp. strain tm-1, the second of three enzymes involved in the dechlorination of 4-chlorobenzoate to form 4-hydroxybenzoate, has been examined. e(mm) for the enzyme was 12.59. analysis by circular dichroism spectrometry in the far uv indicated that 4-chlorobenzoyl coenzyme a dehalogenase was composed mostly of alpha-helix (56%) with lesser amounts of random coil (21%), beta-turn (13%) and beta ...	2008	17431803
trans-stilbene degradation by arthrobacter sp. sl3 cells.	trans-stilbene degradation was examined by the reaction using resting cells of microorganisms isolated through the enrichment culture using trans-stilbene. the strain sl3, showing the highest trans-stilbene-degrading activity, was identified as arthrobacter sp. one of the reaction products was identified to be cis,cis-muconic acid. arthrobacter sp. sl3 cells also transformed benzaldehyde, benzoic acid and catechol into cis,cis-muconic acid, suggesting that one benzene ring of trans-stilbene was ...	2008	17885812
d-serine dehydratase from chicken kidney: a vertebral homologue of the cryptic enzyme from burkholderia cepacia.	d-serine dehydratase (dsd) catalyses the conversion of d-serine to pyruvate and ammonia. d-serine is a physiological modulator of glutamate neurotransmission in vertebrate brains. in mammals d-serine is degraded by d-amino-acid oxidase, whereas in chicken brain it is degraded by dsd, as we have recently demonstrated [tanaka et al. (2007) anal. biochem. 362, 83-88]. to clarify the roles of dsd in avian species, we purified dsd from chicken kidney. the purified enzyme was a heterodimer consisting ...	2008	17977854
simultaneous mineralization of glyphosate and diuron by a consortium of three bacteria as free- and/or immobilized-cells formulations.	a bacterial consortium able to mineralize two herbicides, glyphosate (pseudomonas 4asw) and diuron (arthrobacter sp. n4 and delftia acidovorans), was cultivated in both a synthetic culture medium without phosphate and a sediment extract medium. in the aim at optimizing glyphosate and diuron mineralization, all the combinations, i.e., free and/or immobilized cells in ca-alginate beads were tested. with the synthetic medium, the simultaneous mineralization of glyphosate and diuron required at leas ...	2008	18026726
chromate reduction by arthrobacter cr47 in biofilm packed bed reactors.	bacterial strain cr47 was isolated from a landfarming process soil sample. it was identified, by 16s rdna sequencing, as arthrobacter sp. the time course of the cr(vi) reduction was monitored in batch operated packed bed biofilm reactors (12 ml void volume) and in recirculating packed bed biofilm reactors (100 ml void volume) inoculated with bacterial strain cr47. the reduction was evaluated with, 30 mg l(-1) cr(vi) laboratory solutions prepared with k2cr2o7 and enriched with glucose-minimal med ...	2008	18063473
isolation and characterization of mesotrione-degrading bacillus sp. from soil.	dissipation kinetics of mesotrione, a new triketone herbicide, sprayed on soil from limagne (puy-de-dôme, france) showed that the soil microflora were able to biotransform it. bacteria from this soil were cultured in mineral salt solution supplemented with mesotrione as sole source of carbon for the isolation of mesotrione-degrading bacteria. the bacterial community structure of the enrichment cultures was analyzed by temporal temperature gradient gel electrophoresis (ttge). the ttge fingerprint ...	2009	19121884
preferential hydrolysis of aberrant intermediates by the type ii thioesterase in escherichia coli nonribosomal enterobactin synthesis: substrate specificities and mutagenic studies on the active-site residues.	the type ii thioesterase enth is a hotdog fold protein required for optimal nonribosomal biosynthesis of enterobactin in escherichia coli. its proposed proofreading activity in the biosynthesis is confirmed by its efficient restoration of enterobactin synthesis blocked in vitro by analogs of the cognate precursor 2,3-dihydroxybenzoate. steady-state kinetic studies show that enth recognizes the phosphopantetheine group and the pattern of hydroxylation in the aryl moiety of its thioester substrate ...	2009	19193103
purification and characterization of arsenite oxidase from arthrobacter sp.	the chemolithoautotroph, arthrobacter sp.15b oxidizes arsenite to arsenate using a membrane bound arsenite oxidase. the enzyme arsenite oxidase is purified to its homogeneity and identified using maldi-tof ms analysis. upon further characterization, it was observed that the enzyme is a heterodimer showing native molecular mass as approximately 100 kda and appeared as two subunits of approximately 85 kda lsu and 14 kda ssu on sds-page. the v(max) and k(m) values of the enzyme was found to be 2.45 ...	2009	19214757
global proteomic analysis of the chromate response in arthrobacter sp. strain fb24.	a global proteomic evaluation of the response of arthrobacter sp. strain fb24 to 5 and 20 mm cr(vi) was conducted using both two-dimensional gel electrophoresis (2-dge) and liquid chromatography coupled with tandem mass spectrometry (lc/lc-ms/ms). the changes in protein expression found with 2-dge indicate alterations in central metabolism and amino acid synthesis. proteome coverage increased from 22% with 2-dge to 71% with lc/lc-ms/ms. the proteins exhibiting the highest levels of expression un ...	2009	19231868
high yield synthesis of 12-aminolauric acid by "enzymatic transcrystallization" of omega-laurolactam using omega-laurolactam hydrolase from acidovorax sp. t31.	the genes encoding omega-laurolactam hydrolases from cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, and sphingomonas sp. u238 were cloned and sequenced. nucleotide and amino acid sequence analysis of the four genes indicated that the primary structures of these omega-laurolactam hydrolases are significantly similar to the 6-aminohexanoate-cyclic-dimer hydrolase (ec 3.5.2.12). these genes were expressed in escherichia coli, and the omega-laurolactam hydrolysing activity of the reco ...	2009	19420719
improvement of catalytic properties of lipase from arthrobacter sp. by encapsulation in hydrophobic sol-gel materials.	in this work, lipase from arthrobacter sp. was immobilized by sol-gel encapsulation to improve its catalytic properties. various silanizing agents including vinyl-trimethoxy silane, octyl-trimethoxy silane, gamma-(methacryloxypropyl)-trimethoxy silane (maptms) and tetraethoxysilane (teos) were chosen as the precursors. among them, maptms was for the first time utilized to encapsulate lipases, and the prepared enzyme by copolymerization of maptms and teos exhibited the highest activity in both th ...	2009	19428242
industrial production of difructose anhydride iii (dfa iii) from crude inulin extracted from chicory roots using arthrobacter sp. h65-7 fructosyltransferase.	a practical, economical, and industrial process for the enzymatic production of difructose anhydride iii (dfa iii) was investigated for crude inulin prepared from chicory roots using arthrobacter sp. h65-7 fructosyltransferase. a comparable level of dfa iii production to that from commercial inulin was obtained using crude inulin, suggesting the feasibility of this production process.	2009	19269589
high rate of n2 fixation by east siberian cryophilic soil bacteria as determined by measuring acetylene reduction in nitrogen-poor medium solidified with gellan gum.	for evaluating n(2) fixation of diazotrophic bacteria, nitrogen-poor liquid media supplemented with at least 0.5% sugar and 0.2% agar are widely used for acetylene reduction assays. in such a soft gel medium, however, many n(2)-fixing soil bacteria generally show only trace acetylene reduction activity. here, we report that use of a n(2) fixation medium solidified with gellan gum instead of agar promoted growth of some gellan-preferring soil bacteria. in a soft gel medium solidified with 0.3% ge ...	2009	19286791
biodegradation of mixed phenolic compounds under high salt conditions and salinity fluctuations by arthrobacter sp. w1.	high salt concentration and salinity fluctuations in wastewater challenge the efficiency of microbial strains used for cleanup of pollutants. in this study, it was investigated that the new isolated arthrobacter sp. w1 degraded mixed phenolic compounds under complex salt conditions. the results showed that arthrobacter sp. w1 was able to utilize various phenolic compounds as carbon source under high salt conditions. it can degrade phenol and p-cresol mixture at 10% nacl, although rates of degrad ...	2009	19156367
[effect of elevation of 1-hydroxy-2-naphthoic acid concentration on the lag phase of arthrobacter sp. k3: kinetic analysis].		2009	19449734
[effect of a novel fibrinolytic enzyme fa-i on thrombosis and thrombolysis].	to study the effect of fa-i, a novel fibrinolytic enzyme isolated from the metabolite of arthrobacter sp. dr-536, on thrombosis and thrombolysis in vivo.	2009	19462909
microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes.	in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ...	2009	19477544
microbial responses to mustard gas dumped in the baltic sea.	microbiological studies were carried out on chemical weapon dump sites in the baltic sea. the effect of mustard gas hydrolysis products (mghps) on marine microbiota and the ability of microorganisms to degrade mghps were studied. many stations at the dump sites demonstrated reduced microbial diversity, and increased growth of species able to use mustard gas hydrolysis products as sole source of carbon. significant amounts of mghp-degrading bacteria were revealed in the near-bottom water. the mgh ...	2009	19481794
batch and continuous biosorption of cu(2+) by immobilized biomass of arthrobacter sp.	the ability of free and polysulphone immobilized biomass of arthrobacter sp. to remove cu(2+) ions from aqueous solution was studied in batch and continuous systems. the langmuir and freundlich isotherm models were applied to the data. the langmuir isotherm model was found to fit the sorption data indicating that sorption was monolayer and uptake capacity (q(o)) was 175.87 and 158.7mg/g for free and immobilized biomass respectively at ph 5.0 and 30 degrees c temperature, which was also confirmed ...	2009	19487070
isolation and characterization of arthrobacter sp. hy2 capable of degrading a high concentration of p-nitrophenol.	a soil bacterium strain, capable of using p-nitrophenol (pnp) as its sole source of carbon and energy, was isolated by enrichment on minimal salt medium (msm). on the basis of a phylogenetic analysis of 16s rrna gene sequences the bacterium is a species of arthrobacter, closely related to arthrobacter ureafaciens dsm 20126. this strain has an unusually high substrate tolerance for pnp degradation in msm. greatest degradation of pnp was observed at 30 degrees c and under slightly alkaline ph (ph ...	2009	19540107
genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition.	in this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for plant growth promoting (pgp) traits at higher salt (nacl) concentrations (2, 4, 6, and 8%). the results revealed that 24 rhizobacterial isolates were tolerant at 8% nacl. although all the 24 salt tolerable isolates produced indole-3-acetic acid (iaa), while 10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. however, only three isol ...	2009	19701667
analysis of two gene clusters involved in the degradation of 4-fluorophenol by arthrobacter sp. strain if1.	arthrobacter sp. strain if1 is able to grow on 4-fluorophenol (4-fp) as a sole source of carbon and energy. to clone the 4-fp degradation genes, dna libraries were constructed and screened with a probe obtained by pcr using primers designed on the basis of conserved regions of aromatic two-component monooxygenases. sequencing of positive clones yielded two gene clusters, each harboring a gene encoding a monooxygenase with high sequence similarity to the oxygenase component of 4-nitrophenol and 4 ...	2009	19837837
high-level chromate resistance in arthrobacter sp. strain fb24 requires previously uncharacterized accessory genes.	the genome of arthrobacter sp. strain fb24 contains a chromate resistance determinant (crd), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. the crd includes chra, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino and carboxy termini of chrb, a putative regulatory protein. there are also three novel genes that have not been previously associated with chromate resistance in other bacteria; they encode a ...	2009	19758450
a new beta-galactosidase with a low temperature optimum isolated from the antarctic arthrobacter sp. 20b: gene cloning, purification and characterization.	a psychrotrophic bacterium producing a cold-adapted beta-galactosidase upon growth at low temperatures was classified as arthrobacter sp. 20b. a genomic dna library of strain 20b introduced into escherichia coli top10f' and screening on x-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside)-containing agar plates led to the isolation of beta-galactosidase gene. the beta-galactosidase gene (bgas) encoding a protein of 1,053 amino acids, with a calculated molecular mass of 113,695 kda. analys ...	2009	19771412
atrazine and terbuthylazine mineralization by an arthrobacter sp. isolated from a sugarcane-cultivated soil in kenya.	a tropical soil from a kenyan sugarcane-cultivated field showed a very high capability to mineralize (14)c-ring-labeled atrazine. in laboratory experiments this soil mineralized about 90% of the applied atrazine within 98 d. the atrazine-degrading microbial community was enriched in liquid cultures containing atrazine as the sole n source and 100 mgl(-1) glucose as additional c source. from the enrichment culture a bacterial strain was isolated and identified by comparative sequence analysis of ...	2009	19674769
monitoring enzyme catalysis in the multimeric state: direct observation of arthrobacter 4-hydroxybenzoyl-coenzyme a thioesterase catalytic complexes using time-resolved electrospray ionization mass spectrometry.	the ability to examine real-time reaction kinetics for multimeric enzymes in their native state may offer unique insights into understanding the catalytic mechanism and its interplay with three-dimensional structure. in this study, we have used a time-resolved electrospray mass spectrometry approach to probe the kinetic mechanism of 4-hydroxybenzoyl-coenzyme a (4-hba-coa) thioesterase from arthrobacter sp. strain su in the millisecond time domain. intact tetrameric complexes of 4-hba-coa thioest ...	2009	19635449
arthrobacter phenanthrenivorans sp. nov., to accommodate the phenanthrene-degrading bacterium arthrobacter sp. strain sphe3.	a novel phenanthrene-degrading bacterium, designated strain sphe3(t), was isolated from a creosote-contaminated soil in greece. cells were non-motile, gram-positive, aerobic, and rod- to coccus-shaped. the strain was isolated on the basis of formation of a clear zone on agar plates sprayed with phenanthrene. optimal growth occurred at 30 degrees c. the g+c content of the dna was 65.7 mol%. the polar lipid pattern of strain sphe3(t) consisted of phosphatidylethanolamine, phosphatidylglycerol and ...	2009	19196765
effect of immobilization of a bacterial consortium on diuron dissipation and community dynamics.	this work intended to study the relationship between diuron herbicide dissipation and the population dynamics of co-cultivated delftia acidovorans wdl34 (wdl34) and arthrobacter sp. n4 (n4) for different cell formulations: free cells or immobilization in ca-alginate beads of one or both strains. gfp-tagged wdl34 and n4 gram staining allowed analyzing the cell growth and distribution of each strain in both beads and culture medium in the course of the time. compared to the free cell co-culture of ...	2009	19386487
a novel aryl acylamidase from nocardia farcinica hydrolyses polyamide.	an alkali stable polyamidase was isolated from a new strain of nocardia farcinica. the enzyme consists of four subunits with a total molecular weight of 190 kda. the polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hydrolyzed different soluble amides to the corresponding acid. treatment of polyamide 6 with this amidase led to an increased hydrophilicity based on rising height and tensiometry mea ...	2009	18942140
arthrobacter sp. lipase immobilization for preparation of enantiopure masked beta-amino alcohols.	recent reports on immobilization of lipase from arthrobacter sp. (abl, mtcc 5125; iiim isolate) on insoluble polymers have shown altered properties including stability and enantioselectivity. present work demonstrates a facile method for the preparation of enantiopure beta-amino alcohols by modulation of abl enzyme properties via immobilization on insoluble as well as soluble supports using entrapment/covalent binding techniques. efficacies of immobilized abl on insoluble supports prepared from ...	2009	19081255
plasmid as a measure of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid.	the purpose of this research was to pursuit the quantification of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid (2,4-d) by detecting and quantifying a prominent 2,4-d degradation encoding plasmid. batch reactor acclimation, de-acclimation, and re-acclimation tests were conducted during which periods the courses of 2,4-d dissipation and plasmid evolution were quantitatively measured. pure cultures of bacterial strains were detected to give rise to a plasmid approximately the s ...	2009	18930390
[monosaccharide composition of exopolymer complex of bacteria-destructors of protective coatings].	monosaccharide composition of exopolymer complex (epc) ofbacteria-destructors of protective coatings pseudomonas sp. t/, pseudomonas sp. 109, arthrobacter sp. 102 has been studied. it is established that monosaccharide composition of exopolymer complex of bacteria is different and depends on the bacteria growth model. it is mainly represented by penthoses and hexoses. glucose, galactose, arabinose, which are determined in epc of biofilm and plankton of all studied bacteria, are dominating monosa ...	2009	19938612
membrane bioreactor treatment of a simulated metalworking fluid wastewater containing ethylenediaminetetraacetic acid and dicyclohexylamine.	membrane bioreactors (mbrs) have been installed at automotive plants to treat metalworking fluid (mwf) wastewaters, which are known to contain toxic and/or recalcitrant organic compounds. a laboratory study was conducted to evaluate treatment of a simulated wastewater prepared from a semisynthetic mwf, which contains two such compounds, dicyclohexylamine (dcha) and ethylenediaminetetraacetic acid (edta). primary findings were as follows: during stable operating periods, almost all chemical oxyge ...	2009	19445324
isolation of an isocarbophos-degrading strain of arthrobacter sp. scl-2 and identification of the degradation pathway.	isocarbophos is a widely used organophosphorus insecticide that has caused environmental pollution in many areas. however, degradation of isocarbophos by pure cultures has not been extensively studied, and the degradation pathway has not been determined. in this paper, a highly effective isocarbophos-degrading strain, scl-2, was isolated from isocarbophos-polluted soil. strain scl-2 was preliminarily identified as arthrobacter sp. based on its morphological, physiological, and biochemical proper ...	2009	19996699
[isolation and identification of four dbp-degrading strains and molecular cloning of the degradation genes].	four di-butyl-phthalate(dbp)-degrading bacterial strains, jdc-1, jdc-8, jdc-9 and jdc-12, were isolated from soil. the strains were gram positive. the 16s rrna sequence analysis revealed that the four strains had similarities of 99% with arthrobacter sp.. according to the morphologic, physiobiochemical characteristics and the analysis of their 16s rrna, all the four strains were identified as arthrobacter sp.. a 900 bp dna fragment was obtained from the four strains by pcr amplified and clone. w ...	2009	19927831
optimization of physical parameters for lipase production from arthrobacter sp. bgcc#490.	the physical parameters for the production of thermostable, alkaline lipase from arthrobacter sp. bgcc# 490 were optimized using response surface methodology (rsm), employing face centered central composite design (fcccd). the design was employed by selecting ph, temperature and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by rsm. a second-order quadratic model and response surface method showed that the optimum conditions for lipa ...	2009	19517996
a new cold-adapted beta-d-galactosidase from the antarctic arthrobacter sp. 32c - gene cloning, overexpression, purification and properties.	the development of a new cold-active beta-d-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production.	2009	19631003
comparative study of properties of immobilized lipase onto glutaraldehyde-activated amino-silica gel via different methods.	the enzyme-aggregate coating method was performed to immobilize arthrobacter sp. lipase in order to achieve better catalytic properties comparable to the conventional covalent attachment and covalent attachment plus cross-linking. the glutaraldehyde-activated amino-silica gel which was synthesized by sol-gel technique was used as the support, and the catalytic characteristics of the lipase preparations were tested in the asymmetric acylation of 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-o ...	2010	20399626
homology modeling and docking study of xanthine oxidase of arthrobacter sp. xl26.	hyperuricemia is a condition of defective purine metabolism characterized with elevated serum uric acid (ua) level that further leads to gout and gouty nephrolithiasis disorders. gout is a world wide distributed rheumatic disease comprises 1% of the total population and still is in increasing state. one of the factors contributing to overproduction of ua is the hydroxylation of xanthine catalyzed by xanthine oxidase (xo). in the present study, 3d modeling of arthrobacter sp. xl26 (xodb) protein ...	2010	20403378
weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution.	several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ...	2010	20525872
influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments.	because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ...	2010	20553043
high-density polyethylene (hdpe)-degrading potential bacteria from marine ecosystem of gulf of mannar, india.	assessment of high-density polyethylene (hdpe)-degrading bacteria isolated from plastic waste dumpsites of gulf of mannar.	2010	20586938
study on the removal of indoor vocs using biotechnology.	with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ...	2010	20598440
cg2091 encodes a polyphosphate/atp-dependent glucokinase of corynebacterium glutamicum.	the corynebacterium glutamicum gene cg2091 is encoding a polyphosphate (polyp)/atp-dependent glucokinase (ppgk). previous work demonstrated the association of ppgk to polyp granules. the deduced amino acid sequence of ppgk shows 45% sequence identity to polyp/atp glucomannokinase of arthrobacter sp. strain km and 50% sequence identity to polyp glucokinase of mycobacterium tuberculosis h37rv. ppgk from c. glutamicum was purified from recombinant escherichia coli. polyp was highly preferred over a ...	2010	20379711
complete degradation of di-n-octyl phthalate by biochemical cooperation between gordonia sp. strain jdc-2 and arthrobacter sp. strain jdc-32 isolated from activated sludge.	two bacterial strains were isolated from activated sludge using mixtures of phthalic acid esters (paes) as the sole source of carbon and energy. one of the isolates was identified as gordonia sp. strain jdc-2 and the other as arthrobacter sp. strain jdc-32, mainly through 16s rrna gene sequence analysis. gordonia sp. strain jdc-2 rapidly degraded di-n-octyl phthalate (dop) into phthalic acid (pa), which accumulated in the culture medium. arthrobacter sp. strain jdc-32 degraded pa but not dop. th ...	2010	19959291
growth of arthrobacter sp. strain jbh1 on nitroglycerin as the sole source of carbon and nitrogen.	arthrobacter sp. strain jbh1 was isolated from nitroglycerin-contaminated soil by selective enrichment. detection of transient intermediates and simultaneous adaptation studies with potential intermediates indicated that the degradation pathway involves the conversion of nitroglycerin to glycerol via 1,2-dinitroglycerin and 1-mononitroglycerin, with concomitant release of nitrite. glycerol then serves as the source of carbon and energy.	2010	20061454
influence of internal biofilm growth on residual permeability loss in aerobic granular membrane bioreactors.	membrane fouling results in flux decline or transmembrane pressure drop increase during membrane bioreactor (mbr) operation. physical and chemical cleanings are essential to keep an mbr operating at an appropriate membrane flux. considerable residual membrane permeability loss that cannot be removed by conventional cleaning requires membrane replacement. this study demonstrates that an internal biofilm can develop inside a hollow-fiber membrane and can probably account for up to 58.9 and 81.3% o ...	2010	20102183
the biomass yielding process of xenobiotic degradation.	yields of activated sludge and an arthrobacter sp. biomass on organic xenobiotic 2,4-dichlorophenoxyacetic acid (2,4-d) and on the intermediates of selected 2,4-d metabolism pathways were measured. activated sludge yield on 2,4-d was lower by approximately 24-45% compared to the combined yields produced separately by the lower intermediates. for activated sludge, cell synthesis only consumed 33% of the electrons generated from 2,4-d oxidation, while the other 67% were used for energy. the high e ...	2010	20153175
strains of internal biofilm in aerobic granular membrane bioreactors.	this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ...	2010	20306189
arthrobacter sp. strain ku001 isolated from a thai soil degrades atrazine in the presence of inorganic nitrogen sources.	an atrazine degrading bacterium, strain ku001, was obtained from a sugarcane field at the cane and sugar research and development center at the kasetsart university, kamphaeng saen campus, thailand. strain ku001 had a rod-to-coccus morphological cycle during growth. biolog carbon source analysis indicated that the isolated bacterium was arthrobacter histidinolovorans. sequence analysis of pcr product indicated the 16s rrna gene in strain ku001 was 99% identical to the same region in arthrobacter ...	2010	20372034
heavy metal resistance in arthrobacter ramosus strain g2 isolated from mercuric salt-contaminated soil.	present study describes isolation of a multiple metal-resistant arthrobacter ramosus strain from mercuric salt-contaminated soil. the isolate was found to resist and bioaccumulate several metals, such as cadmium, cobalt, zinc, chromium and mercury. maximum tolerated concentrations for above metals were found to be 37, 525, 348, 1530 and 369 microm, respectively. the isolate could also reduce and detoxify redox-active metals like chromium and mercury, indicating that it has great potential in bio ...	2010	20060643
isolation and characterization of a pseudomonas sp. strain iitr01 capable of degrading α-endosulfan and endosulfan sulfate.	to isolate bacteria capable of degrading endosulfan (es) and the more toxic es sulfate and to characterize their metabolites.	2010	20825519
phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal.	the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ...	2010	20833430
identification of chitinases is-chia and is-chib from isoptericola jiangsuensis clg and their characterization.	a 274-bp conserved fragment of chia (chia-cf) was amplified from the genomic dna of isoptericola jiangsuensis clg (dsm 21863, cctcc ab208287) using the specific pcr primers. based on chia-cf sequences, a 5233-bp dna fragment was obtained by self-formed adaptor pcr. dna sequencing analysis revealed there were two contiguous open reading frames coding for the precursors of is-chia [871 amino acids (aa)] and is-chib (561 aa) in the 5233-bp dna fragment. the is-chia and is-chib exhibited 58% and 62% ...	2010	20922373
optimization of electroporation conditions for arthrobacter with plasmid part2.	a prerequisite for genetic studies of arthrobacter is a high efficiency transformation system that allows for dna transfer, transposon mutagenesis, and expression of specific genes. in this study, we develop a detailed electroporation method through a systematic examination of the factors involved in the entire electroporation process. key features of this procedure, including the addition of penicillin to cells during the early log phase of growth and the presence of 0.5m sorbitol in the electr ...	2010	21078345