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direct cellular lysis/protein extraction protocol for soil metaproteomics.we present a novel direct protocol for deep proteome characterization of microorganisms in soil. the method employs thermally assisted detergent-based cellular lysis (sds) of soil samples, followed by tca precipitation for proteome extraction/cleanup prior to liquid chromatography-mass spectrometric characterization. this approach was developed and optimized using different soils inoculated with genome-sequenced bacteria (gram-negative pseudomonas putida or gram-positive arthrobacter chloropheno ...201020954746
transgenic tobacco plants expressing atza exhibit resistance and strong ability to degrade atrazine.atrazine chlorohydrolase (atza) catalyzes hydrolytic dechlorination and can be used in detoxification of atrazine, a herbicide widely employed in the control of broadleaf weeds. in this study, to investigate the potential use of transgenic tobacco plants for phytoremediation of atrazine, atza genes from pseudomonas sp. strain adp and arthrobacter strain ad1 were transferred into tobacco. three and four transgenic lines, expressing atza-adp and atza-ad1, respectively, were produced by agrobacteri ...201020960204
transgenic brassica chinensis plants expressing a bacterial coda gene exhibit enhanced tolerance to extreme temperature and high salinity.transgenic brassica compestris l. spp. chinensis plants expressing a choline oxidase (coda) gene from arthrobacter globiformis were obtained through agrobacterium tumefaciens-mediated transformation. in the transgenic plants, coda gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (elisa) examination, immunogold localization, and (1)h-nuclear magnetic resonance ((1)h-nmr). stress tolerance was evaluated in the t(3) plants under e ...201021043054
biodegradability of bacterial surfactants.this work aimed at evaluating the biodegradability of different bacterial surfactants in liquid medium and in soil microcosms. the biodegradability of biosurfactants by pure and mixed bacterial cultures was evaluated through co(2) evolution. three bacterial strains, acinetobacter baumanni lbbma es11, acinetobacter haemolyticus lbbma 53 and pseudomonas sp. lbbma 101b, used the biosurfactants produced by bacillus sp. lbbma 111a (mixed lipopeptide), bacillus subtilis lbbma 155 (lipopeptide), flavob ...201021053055
optimization of electroporation conditions for arthrobacter with plasmid part2.a prerequisite for genetic studies of arthrobacter is a high efficiency transformation system that allows for dna transfer, transposon mutagenesis, and expression of specific genes. in this study, we develop a detailed electroporation method through a systematic examination of the factors involved in the entire electroporation process. key features of this procedure, including the addition of penicillin to cells during the early log phase of growth and the presence of 0.5m sorbitol in the electr ...201021078345
evaluation of the ltq-orbitrap mass spectrometer for the analysis of polymerase chain reaction products.we have investigated the potential and robustness of the off-line coupling of polymerase chain reaction (pcr) with electrospray ionization mass spectrometry (esi-ms), for further applications in the screening of single-nucleotide polymorphisms (snps). this was based on recently reported data demonstrating that anion-exchange solid-phase extraction was the most efficient technique for efficiently desalting pcr products, with a recovery of ∼70%. results showed that this purification approach effic ...201021080500
root exudates modify bacterial diversity of phenanthrene degraders in pah-polluted soil but not phenanthrene degradation rates.to determine whether the diversity of phenanthrene-degrading bacteria in an aged polycyclic aromatic hydrocarbon (pah) contaminated soil is affected by the addition of plant root exudates, dna stable isotope probing (sip) was used. microcosms of soil with and without addition of ryegrass exudates and with ¹³c-labelled phenanthrene (phe) were monitored over 12 days. phe degradation was slightly delayed in the presence of added exudate after 4 days of incubation. after 12 days, 68% of added phe di ...201021087382
complete biodegradation of 4-fluorocinnamic acid by a consortium comprising arthrobacter sp. strain g1 and ralstonia sp. strain h1.a consortium of the newly isolated bacterial strains arthrobacter sp. strain g1 and ralstonia sp. strain h1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. strain g1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some formation of 4-fluoroacetophenone as a dead-end side product. in the presence of strain h1, complete mineralization of 4-fluorocinnamic acid and release of fluoride were obtained. degradation of 4-fl ...201021097599
lactulose biosynthesis by β-galactosidase from a newly isolated arthrobacter sp.lactulose, a ketose disaccharide, is used in both pharmaceutical and food industries. this study was undertaken to screen and isolate potent β-galactosidase-producing bacteria and to evaluate their enzymatic production of lactulose. soil samples from fruit gardens were collected. one isolate designated las was identified whose cell extract could convert lactose and fructose into lactulose. the 16s rdna gene analysis of las revealed its phylogenetic relatedness to arthrobacter sp. the β-galactosi ...201021104424
degradative potential of stenotrophomonas strain hpc383 having genes homologous to dmp operon.a strain, stenotrophomonas hpc383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through hplc and gc analysis. culture hpc383 could degrade (%) various compounds (1 mm) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% c ...201021123060
the arthrobacter arilaitensis re117 genome sequence reveals its genetic adaptation to the surface of cheese.arthrobacter arilaitensis is one of the major bacterial species found at the surface of cheeses, especially in smear-ripened cheeses, where it contributes to the typical colour, flavour and texture properties of the final product. the a. arilaitensis re117 genome is composed of a 3,859,257 bp chromosome and two plasmids of 50,407 and 8,528 bp. the chromosome shares large regions of synteny with the chromosomes of three environmental arthrobacter strains for which genome sequences are available: ...201021124797
the biodegradation pathway of triethylamine and its biodegradation by immobilized arthrobacter protophormiae cells.a bacterial strain named r4 was isolated from a wastewater treatment pool containing triethylamine (tea) as the sole source of carbon and nitrogen. strain r4 was identified as arthrobacter protophormiae based on 16s rrna gene sequence analysis and morphological and physiological properties. the optimal ph, temperature and concentration of nacl for tea degradation by strain r4 were 7.0, 30°c and 0.5%, respectively. strain r4 could completely degrade 100 mg l(-1) tea to ammonia in 32 h, and could ...201021134717
detection of the reaction intermediates catalyzed by a copper amine oxidase.to reveal the chemical changes and geometry changes of active-site residues that cooperate with a reaction is important for understanding the functional mechanism of proteins. consecutive temporal analyses of enzyme structures have been performed during reactions to clarify structure-based reaction mechanisms. phenylethylamine oxidase from arthrobacter globiformis (agao) contains a copper ion and topaquinone (tpq(ox)). the catalytic reaction of agao catalyzes oxidative deaminations of phenylethy ...201021169693
mechanism of boron tolerance in soil bacteria.boron (b) is toxic to living cells at levels above a certain threshold. we isolated several b-tolerant bacterial strains from soil samples and studied them for possible mechanisms of b tolerance. 16s rrna gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 genera: arthrobacter, rhodococcus, lysinibacillus, algoriphagus, gracilibacillus, and bacillus. these isolates exhibited b-tolerance levels of 80, 100, 150, 300, 450, and 450 mmol/l, r ...201020130690
identification and in vitro deoxynucleotidylation of the terminal protein of the linear plasmid pal1 of arthrobacter nitroguajacolicus rü61a.the plasmid pal1 of arthrobacter nitroguajacolicus rü61a is a linear replicon, characterized by inverted terminal repeats and terminal proteins (tps) covalently bound to its 5'-ends. previous sequence analysis and predictions of possible secondary structures formed by telomeric 3'-overhangs indicated significant differences of the 'left' and 'right' telomere of pal1, raising the question of whether each terminus is recognized by a specific protein. the genes pal1.102 and pal1.103, located close ...201020141532
the biomass yielding process of xenobiotic degradation.yields of activated sludge and an arthrobacter sp. biomass on organic xenobiotic 2,4-dichlorophenoxyacetic acid (2,4-d) and on the intermediates of selected 2,4-d metabolism pathways were measured. activated sludge yield on 2,4-d was lower by approximately 24-45% compared to the combined yields produced separately by the lower intermediates. for activated sludge, cell synthesis only consumed 33% of the electrons generated from 2,4-d oxidation, while the other 67% were used for energy. the high e ...201020153175
one-pot conversion of levan prepared from serratia levanicum nn to difructose anhydride iv by arthrobacter nicotinovorans levan fructotransferase.the newly established difructose anhydride iv (dfa iv) production system is comprised of the effective production of levan from sucrose by serratia levanicum nn, the conversion of the levan into dfa iv by levan fructotransferase from arthrobacter nicotinovorans gs-9, which is highly expressed in an escherichiacoli transformant, and a practical purification step. the chemical properties of dfa iv were also investigated.201020159571
biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms.marine biosphere offers wealthy flora and fauna, which represents a vast natural resource of imperative functional commercial grade products. among the various bioactive compounds, biosurfactant (bs)/bioemulsifiers (be) are attracting major interest and attention due to their structural and functional diversity. the versatile properties of surface active molecules find numerous applications in various industries. marine microorganisms such as acinetobacter, arthrobacter, pseudomonas, halomonas, ...201020172021
c, n, and h isotope fractionation of the herbicide isoproturon reflects different microbial transformation pathways.the fate of pesticides in the subsurface is of great interest to the public, industry, and regulatory authorities. compound-specific isotope analysis (csia) is a promising tool complementary to existing methods for elucidating pesticide degradation reactions. here, we address three different initial biotransformation reactions of the phenylurea herbicide isoproturon (3-(4-isopropylphenyl)-1,1-dimethylurea) in pure culture experiments with bacterial and fungal strains. when analyzing isotopic cha ...201020184312
role of valine 464 in the flavin oxidation reaction catalyzed by choline oxidase.the oxidation of reduced flavin cofactors by oxygen is a very important reaction that is central to the chemical versatility of hundreds of flavoproteins classified as monooxygenases and oxidases. these enzymes are characterized by bimolecular rate constants >or=10(5) m(-1) s(-1) and produce water and hydrogen peroxide, respectively. a hydrophobic cavity close to the reactive flavin c(4a) atom has been previously identified in the 3d structure of monooxygenases but not in flavoprotein oxidases. ...201020184377
crystallization and preliminary crystallographic analysis of manganese(ii)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from bacillus sp. jf8.a thermostable manganese(ii)-dependent 2,3-dihydroxybiphenyl-1,2-dioxygenase derived from bacillus sp. jf8 was crystallized. the initial screening for crystallization was performed by the sitting-drop vapour-diffusion method using a crystallization robot, resulting in the growth of two crystal forms. the first crystal belonged to space group p1, with unit-cell parameters a = 62.7, b = 71.4, c = 93.6 a, alpha = 71.2, beta = 81.0, gamma = 64.0 degrees, and diffracted to 1.3 a resolution. the secon ...201020208161
strains of internal biofilm in aerobic granular membrane bioreactors.this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ...201020306189
esmond e. snell--the pathfinder of b vitamins and cofactors.esmond e. snell (1914-2003) was a giant of b-vitamin and enzyme research. his early research in bacterial nutrition had lead to the discovery of vitamins such as lipoic acid and folic acid, and an anti-vitamin avidin. he developed microbiological assay methods for riboflavin and other vitamins and amino acids, which are still used today. he also investigated the metabolism of vitamins, discovered pyridoxal and pyridoxamine as the active forms of vitamin b(6) and revealed the mechanism of transam ...201020353954
arthrobacter sp. strain ku001 isolated from a thai soil degrades atrazine in the presence of inorganic nitrogen sources.an atrazine degrading bacterium, strain ku001, was obtained from a sugarcane field at the cane and sugar research and development center at the kasetsart university, kamphaeng saen campus, thailand. strain ku001 had a rod-to-coccus morphological cycle during growth. biolog carbon source analysis indicated that the isolated bacterium was arthrobacter histidinolovorans. sequence analysis of pcr product indicated the 16s rrna gene in strain ku001 was 99% identical to the same region in arthrobacter ...201020372034
aerobic biodegradation of a sulfonated phenylazonaphthol dye by a bacterial community immobilized in a multistage packed-bed bac reactor.a microbial community able to aerobically degrade the azo dye acid orange 7 was selected from riparian or lacustrine sediments collected at sites receiving textile wastewaters. three bacterial strains, pertaining to the genera pseudomonas, arthrobacter, and rhizobium, constitute the selected community. the biodegradation of ao7 was carried out in batch-suspended cell culture and in a continuously operated multistage packed-bed bac reactor. the rapid decolorization observed in batch culture, join ...201020376575
structural characterization of the major glycolipids from arthrobacter globiformis and arthrobacter scleromae.arthrobacter is a genus of gram-positive bacteria widely distributed in soil. the ability to catabolize a variety of xenobiotics has shown their potential as a detoxifying agent. recently, arthrobacter has been also recognized as an opportunistic pathogen. glycolipids from a. scleromae, a clinical isolate, and a. globiformis, from soil, were isolated by chloroform-methanol extraction and subsequently purified using column chromatography and high-performance liquid chromatography. structural stud ...201020381794
culture-dependent and culture-independent diversity of actinobacteria associated with the marine sponge hymeniacidon perleve from the south china sea.in this report, the diversity of actinobacteria associated with the marine sponge hymeniacidon perleve collected from a remote island of the south china sea was investigated employing classical cultivation and characterization, 16s rdna library construction, 16s rdna-restriction fragment length polymorphism (rdna-rflp) and phylogenetic analysis. a total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological characteristics for phyl ...201020383659
aepa of pectobacterium is not involved in the regulation of extracellular plant cell wall degrading enzymes production.plant cell wall degrading enzymes (pcwde) are the major virulence determinants in phytopathogenic pectobacterium, and their production is controlled by many regulatory factors. in this study, we focus on the role of the aepa protein, which was previously described to be a global regulator of pcwde production in pectobacterium carotovorum (murata et al. in mol plant microbe interact 4:239-246, 1991). our results show that neither inactivation nor overexpression of aepa affects pcwde production in ...201020386924
[isolation and identification of a fibrinolytic bacterium strain from frozen soil in the tibetan plateau].cardiovascular disease, harmful to human health, is a worldwide disease, for which fibrinolytic enzyme is usually used as an effective remedy. fibrinolytic microbes under extreme environmental conditions may excrete some high efficient and safe fibrinolytic enzymes. so, for obtaining this fibrinolytic enzyme, we isolated fibrinolytic strains from frozen soil in the tibetan plateau.201020387455
glycosylation patterns of human alpha2-macroglobulin: analysis of lectin binding by electron microscopy.human alpha2-macroglobulin (alpha 2m) is a 720 kda glycoprotein that presents two ultrastructural conformations: slow (s-alpha 2m) and fast (f-alpha 2m). alpha 2m acts mainly as a proteinase scavenger, but an immunomodulatory role was also proposed. this work studies the effect of desialylation and deglycosylation on the structure patterns of alpha 2m by ultrastructural analysis of lectin-induced aggregates, which represents a new approach that had never been previously used. transmission electr ...201020399104
comparative study of properties of immobilized lipase onto glutaraldehyde-activated amino-silica gel via different methods.the enzyme-aggregate coating method was performed to immobilize arthrobacter sp. lipase in order to achieve better catalytic properties comparable to the conventional covalent attachment and covalent attachment plus cross-linking. the glutaraldehyde-activated amino-silica gel which was synthesized by sol-gel technique was used as the support, and the catalytic characteristics of the lipase preparations were tested in the asymmetric acylation of 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-o ...201020399626
homology modeling and docking study of xanthine oxidase of arthrobacter sp. xl26.hyperuricemia is a condition of defective purine metabolism characterized with elevated serum uric acid (ua) level that further leads to gout and gouty nephrolithiasis disorders. gout is a world wide distributed rheumatic disease comprises 1% of the total population and still is in increasing state. one of the factors contributing to overproduction of ua is the hydroxylation of xanthine catalyzed by xanthine oxidase (xo). in the present study, 3d modeling of arthrobacter sp. xl26 (xodb) protein ...201020403378
rescuing of the hydride transfer reaction in the glu312asp variant of choline oxidase by a substrate analogue.in the active site of choline oxidase, glu312 participates in binding the trimethylammonium group of choline, thereby positioning the alcohol substrate properly for efficient hydride transfer to the enzyme-bound flavin. previous studies have shown that substitution of glu312 with aspartate results in a perturbed mechanism of hydride transfer, with a 260-fold decrease in the rate associated with the mutation. here, the reaction of alcohol oxidation catalyzed by the glu312asp enzyme has been inves ...201020447376
the microbe-mineral environment and gypsum neogenesis in a weathered polar evaporite.evaporitic deposits are a globally widespread habitat for micro-organisms. the microbe-mineral environment in weathered and remobilized gypsum from exposed mid-ordovician marine evaporite beds in the polar desert of devon island, nunavut, canadian high arctic was examined. the gypsum is characterized by internal green zones of cyanobacterial colonization (dominated by gloeocapsa/aphanothece and chroococcidiopsis spp. morphotypes) and abundant black zones, visible from the surface, that contain p ...201020456500
the type of cheese curds determined the colouring capacity of brevibacterium and arthrobacter species.this study compares the colouring capacity of brevibacterium aurantiacum (ba), brevibacterium bl and arthrobacter species as in relation to deacidified media made from lactic curd (epoisses), mixed curds (munster) and rennet curds (livarot or reblochon). ba colouring capacity proved to be constant, leading to a dark orange colour, irrespective of the deacidified media. however, it gave too dark a colour for reblochon. the strains bl and as were not adapted to the colouring of epoisses deacidifie ...201020462468
calcite-forming bacteria for compressive strength improvement in mortar.microbiological calcium carbonate precipitation (mcp) has been investigated for its ability to improve the compressive strength of concrete mortar. however, very few studies have been conducted on the use of calcite-forming bacteria (cfb) to improve compressive strength. in this study, we discovered new bacterial genera that are capable of improving the compressive strength of concrete mortar. we isolated 4 cfb from 7 environmental concrete structures. using sequence analysis of the 16s rrna gen ...201020467254
engineering of choline oxidase from arthrobacter nicotianae for potential use as biological bleach in detergents.in order to engineer the choline oxidase from arthrobacter nicotianae (an_coda) for the potential application as biological bleach in detergents, the specific activity of the enzyme toward the synthetic substrate tris-(2-hydroxyethyl)-methylammonium methylsulfate (mtea) was improved by methods of directed evolution and rational design. the best mutants (up to 520% wt-activity with mtea) revealed mutations in the fad- (a21v, g62d, i69v) and substrate-binding site (s348l, v349l, f351y). in a separ ...201020467737
arthrobacter endo-beta-n-acetylglucosaminidase shows transglycosylation activity on complex-type n-glycan oxazolines: one-pot conversion of ribonuclease b to sialylated ribonuclease c. 201020486148
[investigation of biotechnological strains of microorganisms for their hygienic standardization].by analyzing the findings and databases on the hygienic standardization of industrial microorganisms, the authors have improved a research program for industrial strains on substantiating their safe exposure levels in workplace and ambient airs. a shortened program is proposed to investigate gram-positive bacteria and nocardioformic actinomycetes (lactobacillus, micrococcus, propionibacterim, brevibacterim, rhodococcus), which have no negative effect. it is necessary to study gram-negative bacte ...201020491276
arthrobacter alpinus sp. nov., a psychrophilic bacterium isolated from alpine soil.an aerobic, gram-reaction-positive, non-motile, psychrophilic bacterium, designated strain s6-3(t), was isolated from alpine soil. cells exhibited a rod-coccus growth cycle and produced a yellow pigment. growth occurred at 1-25 degrees c. phylogenetic analysis based on 16s rrna gene sequences showed that strain s6-3(t) was related to members of the genus arthrobacter, sharing highest sequence similarities with the type strains of arthrobacter psychrolactophilus (97.9 %) and arthrobacter stackebr ...201019880631
removal of organic compounds by a biofilm supported on gac: modelling of batch and column data.the performance of a biofilm of arthrobacter viscosus supported on granular activated carbon on the retention of organic compounds was evaluated. the presence of functional groups on the cell wall surface of the biomass that may interact with the organic compounds was confirmed by fourier transform infrared spectroscopy, to assess the applicability of this system to the removal of those compounds. the batch assays showed that the removal percentage decreases with the increasing initial concentra ...201019882356
x-ray crystallographic analysis of the 6-aminohexanoate cyclic dimer hydrolase: catalytic mechanism and evolution of an enzyme responsible for nylon-6 byproduct degradation.we performed x-ray crystallographic analyses of the 6-aminohexanoate cyclic dimer (acd) hydrolase (nyla) from arthrobacter sp., an enzyme responsible for the degradation of the nylon-6 industry byproduct. the fold adopted by the 472-amino acid polypeptide generated a compact mixed alpha/beta fold, typically found in the amidase signature superfamily; this fold was especially similar to the fold of glutamyl-trna(gln) amidotransferase subunit a (z score, 49.4) and malonamidase e2 (z score, 44.8). ...201019889645
carbon nanotube clusters as universal bacterial adsorbents and magnetic separation agents.the magnetic susceptibility and high bacterial affinity of carbon nanotube (cnt) clusters highlight their great potential as a magnetic bio-separation agent. this article reports the cnt clusters' capability as "universal" bacterial adsorbents and magnetic separation agents by designing and testing a multiwalled carbon nanotube (mwnt) cluster-based process for bacterial capturing and separation. the reaction system consisted of large clusters of mwnts for bacterial capture and an external magnet ...201019856390
genetic diversity of phthalic acid esters-degrading bacteria isolated from different geographical regions of china.thirty-two strains of phthalic acid ester (paes)-degrading bacteria were isolated from thirteen geographically diverse sites by enrichment using mixtures of paes as the sole source of carbon and energy. sequence analyses of the 16s rrna gene indicated that these isolates were from six genera (arthrobacter, gordonia, rhodococcus, acinetobacter, pseudomonas, and delftia). to evaluate the genetic diversity among them, the molecular typing method rep-pcr with primers based on enterobacterial repetit ...201019866373
complete degradation of di-n-octyl phthalate by biochemical cooperation between gordonia sp. strain jdc-2 and arthrobacter sp. strain jdc-32 isolated from activated sludge.two bacterial strains were isolated from activated sludge using mixtures of phthalic acid esters (paes) as the sole source of carbon and energy. one of the isolates was identified as gordonia sp. strain jdc-2 and the other as arthrobacter sp. strain jdc-32, mainly through 16s rrna gene sequence analysis. gordonia sp. strain jdc-2 rapidly degraded di-n-octyl phthalate (dop) into phthalic acid (pa), which accumulated in the culture medium. arthrobacter sp. strain jdc-32 degraded pa but not dop. th ...201019959291
crystal structure analysis of free and substrate-bound 6-hydroxy-l-nicotine oxidase from arthrobacter nicotinovorans.the pathway for oxidative degradation of nicotine in arthrobacter nicotinovorans includes two genetically and structurally unrelated flavoenzymes, 6-hydroxy-l-nicotine oxidase (6hlno) and 6-hydroxy-d-nicotine oxidase, which act with absolute stereospecificity on the l- and d-forms, respectively, of 6-hydroxy-nicotine. we solved the crystal structure of 6hlno at 1.95 a resolution by combined isomorphous/multiple-wavelength anomalous dispersion phasing. the overall structure of each subunit of the ...201020006620
cg2091 encodes a polyphosphate/atp-dependent glucokinase of corynebacterium glutamicum.the corynebacterium glutamicum gene cg2091 is encoding a polyphosphate (polyp)/atp-dependent glucokinase (ppgk). previous work demonstrated the association of ppgk to polyp granules. the deduced amino acid sequence of ppgk shows 45% sequence identity to polyp/atp glucomannokinase of arthrobacter sp. strain km and 50% sequence identity to polyp glucokinase of mycobacterium tuberculosis h37rv. ppgk from c. glutamicum was purified from recombinant escherichia coli. polyp was highly preferred over a ...201020379711
molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques.polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ...201020018377
effects of bacterial treatment at early stages of atlantic cod (gadus morhua l.) on larval survival and development.to assess the effects of bacterial treatment at the earliest stages of cod rearing on the microbial load, larval development and performance, testing three bacterial strains (carnobacterium divergens v41, arthrobacter sp. and enterococcus sp.) in vivo that were previously shown to have inhibitory potential towards fish pathogens in vitro.201019664064
characteristics and function of alcaligenes sp. nbrc 14130 esterase catalysing the stereo-selective hydrolysis of ethyl chrysanthemate.alcaligenes sp. nbrc 14130 was found as a strain hydrolysing a mixture of (+/-)-trans- and (+/-)-cis ethyl chrysanthemates to (1r,3r)-(+)-trans-chrysanthemic acid. the alcaligenes cells also have hydrolytic activity for 6-aminohexanoate-cyclic dimer (6-ahcd, 1,8-diazacyclotetradecane-2,9-dione). the correlation of function on the enzyme from the alcaligenes strain with hydrolysis activities for both ethyl chrysanthemate and 6-ahcd was demonstrated.201019778353
arthrobacter antarcticus sp. nov., isolated from an antarctic marine sediment.a bacterial strain, spc26(t), was isolated from a sediment sample of the southern ocean off antarctica. the strain was gram-staining- and catalase-positive and contained lysine and alanine in the cell-wall peptidoglycan. the major cellular fatty acids were anteiso-c₁₅:₀ (54.92 %), iso-c₁₅:₀ (11.47 %), anteiso-c₁₇:₀ (6.48 %) and anteiso-c₁₅:₁ (6.38 %) and the major menaquinones were mk-8, mk-9 and mk-10. the major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. the g+c cont ...201019783612
soil bacterial communities in constructed wetlands treated with swine wastewater using pcr-dgge technique.marsh-pond-marsh (mpm) constructed wetlands were designed for the treatment of swine wastewater. the goal of this study was to characterize bacterial communities in these wetlands and determine the nutrient removal from influent to effluent. surface soil samples were collected and analyzed by culture-dependent and culture-independent techniques. the results showed that the bacterial colony forming units (cfu) and the average concentrations of total nitrogen, nh(4)(+), total phosphorous (tp) and ...201019822421
hexavalent chromium reduction by free and immobilized cell-free extract of arthrobacter rhombi-re.in the present study, hexavalent chromium (cr(vi)) reduction potential of chromium reductase associated with the cell-free extracts (cfe) of arthrobacter rhombi-re species was evaluated. arthrobacter rhombi-re, an efficient cr(vi) reducing bacterium, was enriched and isolated from a chromium-contaminated site. chromium reductase activity of arthrobacter rhombi-re strain was associated with the cell-free extract and the contribution of extracellular enzymes to cr(vi) reduction was negligible. nad ...201019165627
[isolation, identification and soil remediation of atrazine-degrading strain t3 ab1].to provide new atrazine-degrading strains for atrazine-polluted soil, we isolated the high-efficiency degradation bacterium from contaminated soil, identified with taxonomy, and studied the degrading characteristics and remediation capability of the strain in black soil.201021365918
[effects of nitrogen and phosphorus fertilizer on atrazine degradation and detoxification by degrading strain hb-5].an atrazine-degrading strain hb-5 was used as a bacteria for biodegradation. treatments of soil with nitrogen single, phosphate single and nitrogen phosphate together with hb-5 were carried out for degradation and eco-toxicity test; then, relationship between atrazine degradation rate and soil available nitrogen, available phosphorus were discussed. atrazine residues were determined by hplc; available nitrogen was determined with alkaline hydrolysis diffusion method; available phosphorus was det ...201021229764
a novel replicative enzyme encoded by the linear arthrobacter plasmid pal1.the soil bacterium arthrobacter nitroguajacolicus rü61a contains the linear plasmid pal1, which codes for the degradation of 2-methylquinoline. like other linear replicons of actinomycetes, pal1 is characterized by short terminal inverted-repeat sequences and terminal proteins (tppal1) covalently attached to its 5' ends. tppal1, encoded by the pal1.102 gene, interacts in vivo with the protein encoded by pal1.101. bioinformatic analysis of the pal1.101 protein, which comprises 1,707 amino acids, ...201020675469
weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution.several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ...201020525872
influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments.because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ...201020553043
structural and kinetic studies on the ser101ala variant of choline oxidase: catalysis by compromise.the oxidation of choline catalyzed by choline oxidase includes two reductive half-reactions where fad is reduced by the alcohol substrate and by an aldehyde intermediate transiently formed in the reaction. each reductive half-reaction is followed by an oxidative half-reaction where the reduced flavin is oxidized by oxygen. here, we have used mutagenesis to prepare the ser101ala mutant of choline oxidase and have investigated the impact of this mutation on the structural and kinetic properties of ...201020561507
high-density polyethylene (hdpe)-degrading potential bacteria from marine ecosystem of gulf of mannar, india.assessment of high-density polyethylene (hdpe)-degrading bacteria isolated from plastic waste dumpsites of gulf of mannar.201020586938
investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods.water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ...201020587384
variability of sediment-contact tests in freshwater sediments with low-level anthropogenic contamination--determination of toxicity thresholds.freshwater sediments with low levels of anthropogenic contamination and a broad range of geochemical properties were investigated using various sediment-contact tests in order to study the natural variability and to define toxicity thresholds for the various toxicity endpoints. tests were performed with bacteria (arthrobacter globiformis), yeast (saccharomyces cerevisiae), nematodes (caenorhabditis elegans), oligochaetes (lumbriculus variegatus), higher plants (myriophyllum aquaticum), and the e ...201020594629
prevalence of the gene trzn and biogeographic patterns among atrazine-degrading bacteria isolated from 13 colombian agricultural soils.the following study evaluated the diversity and biogeography of 83 new atrazine-degrading bacteria and the composition of their atrazine degradation genes. these strains were isolated from 13 agricultural soils and grouped according to rep-pcr genomic fingerprinting into 11 major clusters, which showed biogeographic patterns. three clusters (54 strains) belonged to the genus arthrobacter, seven clusters (28 strains) were similar to the genus nocardioides and only one strain was a gram-negative f ...201020597985
16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull.marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ...201021038149
study on the removal of indoor vocs using biotechnology.with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ...201020598440
chemical composition and antibacterial activity of essential oils of lantana camara, ageratum houstonianum and eupatorium adenophorum.essential oils have applications in folk medicine, food preservation, and as feed additives. the essential oils of lantana camara linn. (verbenaceae), ageratum houstonianum mill. (asteraceae) and eupatorium adenophorum spreng. (asteraceae) were analyzed by gas chromatography-mass spectrometry (gcms). in l. camara oil, of the total identified (83.91%) volatile constituents, five constituents [3,7,11-trimethyl-1,6,10-dodecatriene (28.86%), beta-caryophyllene (12.28%), zingiberene (7.63%), gamma-cu ...201020645797
heavy metal resistance in arthrobacter ramosus strain g2 isolated from mercuric salt-contaminated soil.present study describes isolation of a multiple metal-resistant arthrobacter ramosus strain from mercuric salt-contaminated soil. the isolate was found to resist and bioaccumulate several metals, such as cadmium, cobalt, zinc, chromium and mercury. maximum tolerated concentrations for above metals were found to be 37, 525, 348, 1530 and 369 microm, respectively. the isolate could also reduce and detoxify redox-active metals like chromium and mercury, indicating that it has great potential in bio ...201020060643
growth of arthrobacter sp. strain jbh1 on nitroglycerin as the sole source of carbon and nitrogen.arthrobacter sp. strain jbh1 was isolated from nitroglycerin-contaminated soil by selective enrichment. detection of transient intermediates and simultaneous adaptation studies with potential intermediates indicated that the degradation pathway involves the conversion of nitroglycerin to glycerol via 1,2-dinitroglycerin and 1-mononitroglycerin, with concomitant release of nitrite. glycerol then serves as the source of carbon and energy.201020061454
enhanced cyclic adenosine monophosphate production by arthrobacter a302 through rational redistribution of metabolic flux.cyclic adenosine monophosphate (camp) was synthesized through the purine salvage synthesis pathway by arthrobacter a302. results showed that hypoxanthine was the best of the precursors, and the camp concentration reached 4.06 g/l. for inhibition of the glycolytic pathway, sodium fluoride was found the optimal effector, which was further studied on camp production. with the addition of 0.4 g/l of sodium fluoride, the maximal camp concentration reached 11.04 g/l, and the concentrations of lactic a ...201020064709
structural basis for cofactor-independent dioxygenation of n-heteroaromatic compounds at the alpha/beta-hydrolase fold.enzymatic catalysis of oxygenation reactions in the absence of metal or organic cofactors is a considerable biochemical challenge. the co-forming 1-h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (hod) from arthrobacter nitroguajacolicus rü61a and 1-h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase (qdo) from pseudomonas putida 33/1 are homologous cofactor-independent dioxygenases involved in the breakdown of n-heteroaromatic compounds. to date, they are the only dioxygenases suggested to belong to the alp ...201020080731
phylogenetic diversity of alkaline protease-producing psychrotrophic bacteria from glacier and cold environments of lahaul and spiti, india.the diversity of proteolytic bacteria associated with a glacier and cold environment soils from three different locations in lahaul and spiti, india was investigated. two hundred seventeen bacterial strains were isolated in pure culture. subsequently these strains were screened for protease-production and one hundred nine showed protease production. from these protease producing psychrotrophic bacteria twenty showing high enzyme production at low temperature and alkaline ph were characterized an ...201020082368
biotransformation of nicotine by microorganism: the case of pseudomonas spp.several bacterial species are capable of using nicotine, the main alkaloid in tobacco plants, as a substrate for growth. the dominant species include members of two genera, pseudomonas and arthrobacter. the degradation pathway and genetic structure of nicotine catabolism in arthrobacter nicotinovorans were recently reviewed (brandsch appl microbiol biotechnol 69:493-498, 2006). here, we present up-to-date information on biodegradation of nicotine by pseudomonas spp. species in this genus capable ...201020091027
influence of internal biofilm growth on residual permeability loss in aerobic granular membrane bioreactors.membrane fouling results in flux decline or transmembrane pressure drop increase during membrane bioreactor (mbr) operation. physical and chemical cleanings are essential to keep an mbr operating at an appropriate membrane flux. considerable residual membrane permeability loss that cannot be removed by conventional cleaning requires membrane replacement. this study demonstrates that an internal biofilm can develop inside a hollow-fiber membrane and can probably account for up to 58.9 and 81.3% o ...201020102183
replication-involved genes of pal1, the linear plasmid of arthrobacter nitroguajacolicus rü61a--phylogenetic and transcriptional analysis.the 113-kb pal1 is the only arthrobacter linear plasmid known; it has terminal inverted repeats and 5' covalently attached terminal proteins (tps). the latter and a telomere-associated protein (tap) are encoded by plasmid orfs 102 and 101, respectively. as for streptomyces linear replicons, in which both above proteins are instrumental in telomere patching, they are involved in pal1 replication as well. however, the alignment of actinobacterial taps and tps revealed that pal1 and the linear elem ...201021185858
studies of dimethylglycine oxidase isoenzymes in arthrobacter globiformis cells.glycine betaine (gb) could be used by arthrobacter globiformis cells as a sole carbon source. the cells took up this molecule in the low as well as in the high salinity medium. addition of gb to the mineral medium with high salt concentration revealed that gb was also used as an osmoprotectant. dimethylglycine oxidase (dmgo) was involved in the catabolism of gb. two genes for dmgo were detected in a cloned 26267 bp fragment of a. globiformis dna. the genes involved in the tetrahydrofolate-depend ...201021188587
discovery and characterization of d-phenylserine deaminase from arthrobacter sp. tks1.we discovered a d-phenylserine deaminase that catalyzed the pyridoxal 5'-phosphate (plp)-dependent deamination reaction from d-threo-phenylserine to phenylpyruvate in newly isolated arthrobacter sp. tks1. the enzyme was partially purified, and its n-terminal amino acid sequence was analyzed. based on the sequence information, the gene encoding the enzyme was identified and expressed in escherichia coli. the expressed protein was purified to homogeneity and characterized. the enzyme consisted of ...201021190106
bacterial diversity from benthic mats of antarctic lakes as a source of new bioactive metabolites.during the micromat project, the bacterial diversity of microbial mats growing in the benthic environment of antarctic lakes was accessed for the discovery of novel antibiotics. in all, 723 antarctic heterotrophic bacteria belonging to novel and/or endemic taxa in the +¦-, +¦- and +¦-subclasses of the proteobacteria, the bacteroidetes branch, and of the high and low percentage g+c gram-positives, were isolated, cultivated in different media and at different temperatures, and then screened for th ...200921798170
identification and characterization of bacteria isolated under selective pressure of volatile organic compounds.several bacterial strains tolerant to volatile organic compounds (vocs) were isolated from the air emissions of a typical industrial wood painting plant. four strains that grew vigorously on a mineral salt basal medium and voc-saturated atmosphere were identified using biolog and fatty acids analysis. a 16s rdna sequence comparison was also performed on three of the four strains. their phylogenetic positions were respectively: mc8m6 of the genus arthrobacter mcep3 of the genus microbacterium, mc ...200920112870
genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils.three parathion-degrading bacteria and eight pairs of bacteria showing syntrophic metabolism of parathion were isolated from rice field soils, and their genetic and phenotypic characteristics were investigated. the three isolates and eight syntrophic pairs were able to utilize parathion as a sole source of carbon and energy, producing p-nitrophenol as the intermediate metabolite during the complete degradation of parathion. analysis of 16s rrna gene sequence indicated that the isolates were rela ...200920075637
c29 olefinic hydrocarbons biosynthesized by arthrobacter species.arthrobacter aurescens tc1, arthrobacter chlorophenolicus a6, arthrobacter crystallopoietes, and arthrobacter oxydans produce long-chain monoalkenes, predominantly cis-3,25-dimethyl-13-heptacosene. four other arthrobacter strains did not form alkenes. the level of cis-3,25-dimethyl-13-heptacosene in arthrobacter chlorophenolicus a6 remained proportional to cell mass during growth. cis-3,25-dimethyl-13-heptacosene did not support growth of a. chlorophenolicus a6.200919168653
mating triggers dynamic immune regulations in wood ant queens.mating can affect female immunity in multiple ways. on the one hand, the immune system may be activated by pathogens transmitted during mating, sperm and seminal proteins, or wounds inflicted by males. on the other hand, immune defences may also be down-regulated to reallocate resources to reproduction. ants are interesting models to study post-mating immune regulation because queens mate early in life, store sperm for many years, and use it until their death many years later, while males typica ...200919170815
gene cloning, expression, and characterization of a novel trehalose synthase from arthrobacter aurescens.trehalose synthase (tres) is an intramolecular transglycosylase. it specially catalyzes the conversion of maltose and trehalose. in this study, a novel tres gene, which had a length of 1,797 bp and encoded 598 amino acids, was cloned from arthrobacter aurescens cgmcc 1.1892 and expressed in escherichia coli. thin layer chromatography results indicated that it could catalyze the conversion between maltose and trehalose in one step. however, the ion chromatography results showed that, as a byprodu ...200919172263
targeting prokaryotic choline oxidase into chloroplasts enhance the potential of photosynthetic machinery of plants to withstand oxidative damage.chloroplasts from plants of transgenic lines expressing prokaryotic choline oxidase gene (the coda(ps) gene; genbank accession number-ay589052) and wild-type of chickpea and indian mustard were evaluated for their efficacy to withstand photoinhibitory damage, by exposing them to high light intensity ( approximately 1200micromolm(-2)s(-1) photon flux density) at 10 and 25 degrees c. western analysis confirmed presence of choline oxidase in chloroplasts of only transgenic lines. the loss in ps ii ...200919186067
improvement of steroid biotransformation with hydroxypropyl-beta-cyclodextrin induced complexation.the inclusion complexes induced by cyclodextrins and its derivates have been shown previously to enhance the biotransformation of hydrophobic compounds. using hydroxypropyl-beta-cyclodextrin (hp-beta-cd; 20% w/v), the water solubility of cortisone acetate increased from 0.039 to 7.382 g l(-1) at 32 degrees c. the solubilization effect of hp-beta-cd was far superior to dimethylformamide (dmf) and ethanol. the dissolution rate also significantly increased in the presence of hp-beta-cd. the enzymat ...200919189059
preferential hydrolysis of aberrant intermediates by the type ii thioesterase in escherichia coli nonribosomal enterobactin synthesis: substrate specificities and mutagenic studies on the active-site residues.the type ii thioesterase enth is a hotdog fold protein required for optimal nonribosomal biosynthesis of enterobactin in escherichia coli. its proposed proofreading activity in the biosynthesis is confirmed by its efficient restoration of enterobactin synthesis blocked in vitro by analogs of the cognate precursor 2,3-dihydroxybenzoate. steady-state kinetic studies show that enth recognizes the phosphopantetheine group and the pattern of hydroxylation in the aryl moiety of its thioester substrate ...200919193103
effects of proteus vulgaris growth on the establishment of a cheese microbial community and on the production of volatile aroma compounds in a model cheese.to investigate the impact of proteus vulgaris growth on a multispecies ecosystem and on volatile aroma compound production during cheese ripening.200919426267
endo-beta-n-acetylglucosaminidase-catalyzed polymerization of beta-glcp-(1-->4)-glcpnac oxazoline: a revisit to enzymatic transglycosylation.an alternative synthesis of beta-glcp-(1-->4)-glcpnac oxazoline is described, and its enzymatic reaction with the endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) was re-investigated. under normal transglycosylation conditions with a catalytic amount of enzyme, endo-a showed only marginal activity for transglycosylation with the disaccharide oxazoline, consistent with our previous observations. however, when used in a relatively large quantity, endo-a could promote the ...200919193364
proposal of sinomonas flava gen. nov., sp. nov., and description of sinomonas atrocyanea comb. nov. to accommodate arthrobacter atrocyaneus.a novel actinomycete strain, designated cw 108(t), was isolated from a forest soil in anhui province, china. the cells were strictly aerobic, non-motile, bent rods. the strain grew optimally at 30-37 degrees c and ph 6.0-8.0. chemotaxonomically, the peptidoglycan type was a3alpha, the cell-wall sugars contained galactose, mannose and ribose, the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylmonomethylethanolamine, the major fatty acids were ...200919196763
arthrobacter phenanthrenivorans sp. nov., to accommodate the phenanthrene-degrading bacterium arthrobacter sp. strain sphe3.a novel phenanthrene-degrading bacterium, designated strain sphe3(t), was isolated from a creosote-contaminated soil in greece. cells were non-motile, gram-positive, aerobic, and rod- to coccus-shaped. the strain was isolated on the basis of formation of a clear zone on agar plates sprayed with phenanthrene. optimal growth occurred at 30 degrees c. the g+c content of the dna was 65.7 mol%. the polar lipid pattern of strain sphe3(t) consisted of phosphatidylethanolamine, phosphatidylglycerol and ...200919196765
glycosynthases enable a highly efficient chemoenzymatic synthesis of n-glycoproteins carrying intact natural n-glycans.homogeneous n-glycoproteins carrying defined natural n-glycans are essential for detailed structural and functional studies. the transglycosylation activity of the endo-beta-n-acetylglucosaminidases from arthrobacter protophormiae (endo-a) and mucor hiemalis (endo-m) holds great potential for glycoprotein synthesis, but the wild-type enzymes are not practical for making glycoproteins carrying native n-glycans because of their predominant activity for product hydrolysis. in this article, we repor ...200919199609
purification and characterization of arsenite oxidase from arthrobacter sp.the chemolithoautotroph, arthrobacter sp.15b oxidizes arsenite to arsenate using a membrane bound arsenite oxidase. the enzyme arsenite oxidase is purified to its homogeneity and identified using maldi-tof ms analysis. upon further characterization, it was observed that the enzyme is a heterodimer showing native molecular mass as approximately 100 kda and appeared as two subunits of approximately 85 kda lsu and 14 kda ssu on sds-page. the v(max) and k(m) values of the enzyme was found to be 2.45 ...200919214757
thermal analysis of whole bacterial cells exposed to potassium permanganate using differential scanning calorimetry: a biphasic dose-dependent response to stress.the dsc technique applied to the study of the toxic impact of permanganate on bacterial cell culture detected the lack of linearity in the dose-response effect. the results were confirmed by the traditional assay of colony forming ability. the changed pattern of thermal spectra of a. oxydans at permanganate treatment, the measurement of the total heat capacity and the temperature of dnp complex demonstrate the possibility to verify the toxic impact in dependence of concentrations value.200919219375
[effect of a novel fibrinolytic enzyme fa-i on thrombosis and thrombolysis].to study the effect of fa-i, a novel fibrinolytic enzyme isolated from the metabolite of arthrobacter sp. dr-536, on thrombosis and thrombolysis in vivo.200919462909
global proteomic analysis of the chromate response in arthrobacter sp. strain fb24.a global proteomic evaluation of the response of arthrobacter sp. strain fb24 to 5 and 20 mm cr(vi) was conducted using both two-dimensional gel electrophoresis (2-dge) and liquid chromatography coupled with tandem mass spectrometry (lc/lc-ms/ms). the changes in protein expression found with 2-dge indicate alterations in central metabolism and amino acid synthesis. proteome coverage increased from 22% with 2-dge to 71% with lc/lc-ms/ms. the proteins exhibiting the highest levels of expression un ...200919231868
bacterial communities of tyre monofill sites: growth on tyre shreds and leachate.to investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria.200919239530
structural basis and catalytic mechanism for the dual functional endo-beta-n-acetylglucosaminidase a.endo-beta-n-acetylglucosaminidases (engases) are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation reactions. recently, these enzymes have become the focus of intense research because of their potential for synthesis of glycopeptides. we have determined the 3d structures of an engase from arthrobacter protophormiae (endo-a) in 3 forms, one in native form, one in complex with man(3)glcnac-thiazoline and another in complex with glcnac-asn. the carbohydrate moie ...200919252736
identification of a novel self-sufficient styrene monooxygenase from rhodococcus opacus 1cp.sequence analysis of a 9-kb genomic fragment of the actinobacterium rhodococcus opacus 1cp led to identification of an open reading frame encoding a novel fusion protein, stya2b, with a putative function in styrene metabolism via styrene oxide and phenylacetic acid. gene cluster analysis indicated that the highly related fusion proteins of nocardia farcinica ifm10152 and arthrobacter aurescens tc1 are involved in a similar physiological process. whereas 413 amino acids of the n terminus of stya2 ...200919482928
plasmid as a measure of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid.the purpose of this research was to pursuit the quantification of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid (2,4-d) by detecting and quantifying a prominent 2,4-d degradation encoding plasmid. batch reactor acclimation, de-acclimation, and re-acclimation tests were conducted during which periods the courses of 2,4-d dissipation and plasmid evolution were quantitatively measured. pure cultures of bacterial strains were detected to give rise to a plasmid approximately the s ...200918930390
endophytic bacterial flora in root and stem tissues of black pepper (piper nigrum l.) genotype: isolation, identification and evaluation against phytophthora capsici.to isolate and identify black pepper (piper nigrum l) associated endophytic bacteria antagonistic to phytophthora capsici causing foot rot disease.200919018963
liquid chromatography-mass spectrometry to study chondroitin lyase action pattern.liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. two commercial enzymes, chondroitinase abc (proteus vulgaris) and chondroitinase acii (arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. next, the action patterns of recombinant lyases, chondroitinase abc from bacteroides thetaiotaomicron (expressed in escherichia coli) and chondroitinase ac from flav ...200918992215
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