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action of levan fructotransferase of arthrobacter ureafaciens on levanoligosaccharides.levan fructotransferase of the bacterium arthrobacter ureafaciens, which produces di-d-fructose 2,6':6,2' dianhydride (difructose anhydride iv) from levan by an intramolecular transfructosylation reaction, was purified to give a single protein band of pi 4.5-4.7 on isoelectric focusing. it had a molecular weight of 128,000 on gel-filtration on sephadex g-200 and 60,000 on sds-polyacrylamide disc gel-electrophoresis, suggesting that the enzyme is composed of two identical subunits. the shortest l ...19836654872
immobilization of proteins on partially hydrolyzed agarose beads.treatment of agarose beads with mild acid (0.2 m hcl, 55 degrees c, several hours) hydrolyzes some of the glycosidic bonds between d-galactosyl residues and 3,6-anhydro-l-galactosyl residues, and thus produces aldehydo-groups useful for immobilization of amino compounds by reductive amination with nacnbh3. more than 20 mg (0.3 mumol) of bovine serum albumin could be coupled per gram of partially hydrolyzed agarose beads. arthrobacter neuraminidase immobilized by this method was useful for desial ...19836660514
the interaction of substrate-related ketals with bacterial and viral neuraminidases.arthrobacter sialophilus neuraminidase catalyzes the hydration of 5-acetamido-2,6-anhydro-3,5-dideoxy-d-glycero-d-galacto-non-2-enonic acid (2,3-dehydro-acneu) with km and kcat values of 8.9 x 10(-4) m and 6.40 x 10(-4) s-1, respectively. the methyl ester of 2,3-dehydro-acneu as well as 2,3-dehydro-4-epi-acneu are also hydrated by the enzyme. the product resulting from the enzymatic hydration of 2,3-dehydro-acneu is n-acetylneuraminic acid. a series of derivatives of 2,3-dehydro-acneu (k1, 1.60 ...19836830254
studies on isoprenoid biosynthesis with bacterial intact cells.for the study on the regulation of isoprenoid biosynthesis with intact cells, some strains of bacteria capable of growing on mevalonate as a sole carbon source were isolated from soil. many of them incorporated [14c]-mevalonate, [14c]isopentenyl- and [14c]farnesyl pyrophosphates into the cells. however, radioactivity was found in their degradation products but not in isoprenoids. addition of [14c]isopentenyl pyrophosphate, farnesyl pyrophosphate and mg2+ ions in combination to the culture of a s ...19836838508
[dynamics of populations of microbial antagonists in nonsterile soil].the interaction between populations was studied with arthrobacter crystallopoietes and streptomyces olivocinereus, an actinomycete producing the antibiotic heliomycin active against gram-positive microorganisms. the two organisms were either cultivated together in a growth medium or the two populations were introduced simultaneously into nonsterile soil at different levels of population density. the antagonism was found in both cases: a. crystallopoietes cells died off when a population of the p ...19836843384
similarities in the induction of synthesis of a cell-surface polypeptide in arthrobacter sp. by near-uv irradiation and photodynamic conditions. 19836856688
quantitation of immunoadsorbed flavoprotein oxidases by luminol-mediated chemiluminescence.the detection of the flavoenzymes 6-hydroxy-l-nicotine oxidase and 6-hydroxy-d-nicotine oxidase at the sub-femtomol level was achieved by coupling the reaction of the immunoadsorbed proteins to the peroxidase-catalysed oxidation of luminol. the h2o2-producing oxidases retained their full activity when bound to the respective immobilized antibodies. this fact allowed the concentration of the enzymes from very dilute solutions and the quantitative assay of their activities in the microu range. due ...19836862382
[regulation of citrate synthase in facultative methylotrophic bacteria].commonly the tca cycle fulfils an anabolic and a catabolic function in case of aerobic chemoorganoheterotrophic nutrition. in methylotrophic growth the tca cycle is dispensable as a bioenergetic pathway. this is reflected by properties of citrate synthase in facultative methylotrophic bacteria. two citrate synthases, a "chemoorganoheterotrophic" one, which is inhibited by nadh (or atp in acetobacter mb 58), and a "methylotrophic" one, which is not or less affected by energy indicators, were foun ...19836880250
the specificity of viral and bacterial sialidases for alpha(2-3)- and alpha(2-6)-linked sialic acids in glycoproteins.the anomeric specificity of six sialidases (vibrio cholerae, arthrobacter ureafaciens, clostridium perfringens, newcastle disease virus, fowl plague virus and influenza a2 virus sialidases) was assessed with sialylated antifreeze glycoprotein, ovine submandibular gland glycoprotein and alpha 1-acid glycoprotein, resialylated specifically in alpha(2-3) or alpha(2-6) linkage with n-acetylneuraminic acid or n-glycolylneuraminic acid using highly purified sialyltransferases. the rate of release of s ...19836301560
[the phosphoketolase system of carbohydrate cleavage in saprophytic mycobacteria]. 19836435980
[taxonomic position of the genus mycococcus].an attempt to elucidate the taxonomic position of the genera mycococcus has been made. for this purpose the full range of the taxonomically important properties were investigated among microbial cultures preliminary identified as mycococci. as a result of comparison mycococcus with micrococcus the taxonomic status of the genus mycococcus was no longer confirmed. on the grounds of the published data and the author's experimental results it was concluded that genus micrococcus was closely related ...19836405816
purification and properties of a nad-linked 1,2-propanediol dehydrogenase from propane-grown pseudomonas fluorescens nrrl b-1244.nad-dependent 1,2-propanediol dehydrogenase (ec 1.1.1.4) activity was detected in cell-free crude extracts of various propane-grown bacteria. the enzyme activity was much lower in 1-propanol-grown cells than in propane-grown cells of pseudomonas fluorescens nrrl b-1244, indicating that the enzyme may be inducible by metabolites of propane subterminal oxidation. 1,2-propanediol dehydrogenase was purified from propane-grown ps. fluorescens nrrl b-1244. the purified enzyme fraction shows a single-p ...19836407398
identification key for coryneform bacteria derived by numerical taxonomic studies.six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ...19836413644
biology of aerobic carbon monoxide-oxidizing bacteria. 19836416144
microbial degradation of synthetic organochlorine compounds. 19836416886
isolation of mannan-protein complexes from viable cells of saccharomyces cerevisiae x2180-1a wild type and saccharomyces cerevisiae x2180-1 a-5 mutant strains by the action of zymolyase-60,000.the viable whole cells of saccharomyces cerevisiae x2180-1a wild type and its mannan mutant strain s. cerevisiae x2180-1a-5, were treated with an arthrobacter sp. beta-1,3-glucanase in the presence of a serine protease inhibitor, phenyl-methylsulfonyl fluoride. fractionation of the solubilized materials of each strain with cetavlon (cetyltrimethylammonium bromide) yielded one mannan-protein complex. molecular weights of these complexes were almost the same as that of the mannoprotein of the muta ...19836355061
use of specialized microbial strains in the treatment of industrial waste and in soil decontamination. 19836357840
detoxification of pesticides by microbial enzymes. 19836357841
a phylogenetic analysis of the family dermatophilaceae.the comparative analysis of the 16s ribosomal ribonucleic acid (rrna) of geodermatophilus obscurus dsm 43060 and dermatophilus congolensis dsm 43037 revealed that these members of the family dermatophilaceae were only remotely related. while g. obscurus represented an individual and separate line of descent within the phylogenetically defined order actinomycetales, d. congolensis was closely related to representatives of arthrobacter, micrococcus, cellulomonas, brevibacterium, promicromonospora ...19836195302
siderophore production by vibrio vulnificus.previous studies in our laboratory, as well as clinical evidence, have suggested that increased iron levels in the host may be important in infections caused by the halophilic pathogen vibrio vulnificus. to study iron acquisition, we induced siderophore production by growth in a low-iron medium, and biochemical testing indicated the production of both hydroxamate- and phenolate-type siderophores. the siderophores were extracted from growth filtrates with ethyl acetate (for phenolates) and phenol ...19836223882
siderophore-mediated iron uptake in different strains of anabaena sp.anabaena sp. strain 6411, which produces the dihydroxamate siderophore schizokinen to facilitate iron uptake, is also capable of using the related siderophore aerobactin. the two siderophores compete for the same iron transport system, but there is a markedly higher affinity for ferric schizokinen than for ferric aerobactin. the trihydroxamate siderophore ferrioxamine b is far less effective as an iron donor in this organism. anabaena sp. strain 7120 appears to be closely related to strain 6411. ...19836227608
a sulfated polysaccharide produced by an arthrobacter species.a new sulfated polysaccharide was isolated from the culture supernatant of a strain of arthrobacter sp. the polysaccharide purified with quaternary ammonium salts consists of d-galactose, d-glucose, sulfate, phosphorus, glucosamine, muramic acid, alanine, glutamic acid, glycine, and ll-diaminopimelic acid in a molar ratio of 56 : 9.0 : 68 : 6.4 : 2.0 : 1.1 : 2.1 : 1.0 : 1.2 : 1.2. the presence of the two amino sugars and four amino acids suggests that the polysaccharide, which is principally a g ...19826298190
the effect of gyrase inhibitors and cyclic amp on induction and glucose repression of the 6-hydroxy-nicotine oxidases in arthrobacter oxidans.the induction by d,l-nicotine of the enantiozymes 6-hydroxy-l-nicotine oxidase and 6-hydroxy-d-nicotine oxidase in arthrobacter oxidans was differently affected by the inhibitors of escherichia coli gyrase, novobiocin and nalidixic acid. these compounds inhibited 6-hydroxy-l-nicotine oxidase induction slightly, but led to an increase in the level of 6-hydroxy-d-nicotine oxidase activity. furthermore, the specific repression by glucose of 6-hydroxy-d-nicotine oxidase synthesis was not abolished b ...19826303240
[differentiation of the genera of coryneform bacteria synthesizing amino acids and nucleotides].the morphological, cultural, physio-biochemical and chemotaxonomic properties as well as the content of gc in dna were studied in coryneform bacterial strains producing amino acids and nucleotides. it has been shown that brevibacterium ammoniagenes vkm 672, b. flavum 317a, b. stationis ccm 317 and corynebacterium vsti 301 should be assigned to the genus corynebacterium. the taxonomic significance of chemotaxonomic and physio-biochemical properties is discussed on the basis of the results obtaine ...19826803106
numerical classification of some rhodococci, corynebacteria and related organisms.nineteen strains of corynebacterium sensu stricto, 23 received as corynebacterium equi or rhodococcus equi, marker cultures of arthrobacter, brevibacterium, bacterionema matruchotii, cellulomonas flavigena, kurthia zopfii, listeria denitrificans, microbacterium lacticum, rhodococcus rubropertinctus and 88 representatives of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were the subject of numerical phenetic analyses using 92 characters. the data were examined using the simple m ...19826811696
involvement of plasmids in total degradation of chlorinated biphenyls.acinetobacter sp. strain p6 has previously been reported to utilize biphenyl (bp) and chlorinated bps, with accumulation of corresponding chlorobenzoic acids. arthrobacter sp. strain m5 was isolated as a contaminant in the culture of acinetobacter sp. strain p6 growing on 4-chlorobiphenyl and showed properties similar to p6 in the degradation of chlorinated bps. both strains harbored an identical plasmid of 53.7 megadaltons. these strains spontaneously lost the ability to utilize bp and 4-chloro ...19826814360
purification and characterization of beta-galactoside (alpha 2 leads to 6)sialyltransferase from rat liver and hepatomas.asialofetuin sialyltransferase from triton x-100 extracts of rat liver was resolved by phosphocellulose chromatography into two fractions, designated i and ii in order of elution. when previously treated with arthrobacter ureafaciens neuraminidase, fraction i eluted at about the same position as ii while no alteration occurred in ii. primary rat hepatomas contained only a single asialofetuin sialyltransferase, identical to fraction i in chromatographic behavior. transferases i and ii were purifi ...19826751821
factors determining steric course of enzymic glycosylation reactions: glycosyl transfer products formed from 2,6-anhydro-1-deoxy-d-gluco-hept-1-enitol by alpha-glucosidases and an inverting exo-alpha-glucanase.glycosyl transfer products were formed from 2,6-anhydro-1-deoxy-d-gluco-hept-1-enitol (heptenitol) by purified alpha-glucosidases from candida tropicalis and rice and by an inverting exo-alpha-glucanase (glucodextranase) from arthrobacter globiformis. the products were structurally defined through 1h and 13c nmr (nuclear magnetic resonance) spectra of their crystalline per-o-acetates in comparison with those of authentic methyl 1-deoxy-alpha- and methyl 1-deoxy-beta-d-gluco-heptuloside. 1-deoxy- ...19826758847
[microbiological transformation of hydrocortisone by free and immobilized cells]. 19826763460
[factors increasing exoprotease activity in the associative cultures of coryneform bacteria].specific features of the effect of artrobacter citreus on the biosynthesis of fibrinolytic enzymes of nocardia sp. 1 were investigated during the combined cultivation of both microorganisms. it was demonstrated that an increase in the synthesis of fibrinolytic proteases in the mixed culture of a. citreus and n. species 1 was determined by the compounds containing in the a. citreus supernatant rather than by the nutrient deficiency.19827043443
oxygen tolerance of strictly aerobic hydrogen-oxidizing bacteria.growth of various bacteria, especially aerobic hydrogen-oxidizing bacteria, in the presence of 2 to 100% (v/v) oxygen in the gas atmosphere was evaluated. the bacterial strains included alcaligenes eutrophus, a. paradoxus, aquaspirillum autotrophicum, arthrobacter spec. strain 11 x, escherichia coli, arthrobacter globiformis, nocardia opaca, n. autotrophica, paracoccus denitrificans, pseudomonas facilis, p. putida, and xanthobacter autotrophicus. under heterotrophic conditions with fructose or g ...19827049081
contractile effects of bacterial cell walls, their enzymatic digests, and muramyl dipeptides on ileal strips from guinea pigs.cell walls isolated from four bacterial species (streptococcus pyogenes, lactobacillus plantarum, streptomyces gardneri, and nocardia corynebacteriodes), which exhibited the adjuvant effect of stimulating cellular and humoral immune responses against ovalbumin in guinea pigs, caused the slow-starting and long-lasting contraction of guinea pig ileal strips suspended in tyrode solution. in contrast to these cell walls active in immunoadjuvancy, those isolated from five bacterial species (micrococc ...19827056578
substrate and product specificity of arthrobacter sialophilus neuraminidase.arthrobacter sialophilus neuraminidase catalyzes the hydrolysis of n-acetylneuraminyl-alpha-oxygen, nitrogen, and azido glycosides. the most effective of those substrates examined was n-acetylneuraminyl-alpha-4-methylumbelliferylglycoside (acneu-alpha-4-mu; km app, 0.0193 mm; kcat, 136.4 sec-1). the products resulting from the enzymic hydrolysis of n-acetylneuraminyl-alpha-azido-glycoside were n-acetylneuraminic acid and azide ion. n-acetylneuraminyl-alpha-2,3-thiogalactylglycoside and n-acetyln ...19827068676
metabolism of cyclohexaneacetic acid and cyclohexanebutyric acid by arthrobacter sp. strain ca1.a strain of arthrobacter was isolated by enrichment culture with cyclohexaneacetate as the sole source of carbon and grew with a doubling time of 4.2 h. in addition to growing with cyclohexaneacetate, the organism also grew with cyclohexanebutyrate at concentrations not above 0.05%, and with a variety of alicyclic ketones and alcohols. oxidation of cyclohexaneacetate proceeded through formation of the coenzyme a (coa) ester followed by initiation of a beta-oxidation cycle. beta-oxidation was blo ...19827076617
microbial transformations of warfarin: stereoselective reduction by nocardia corallina and arthrobacter species.the microbiological metabolism of warfarin was examined as a model of metabolism in higher organisms, including humans, and to determine the chirality of microbial reductases for application in organic synthesis. nineteen cultures were examined based on their reported abilities to reduce ketonic substrates, and several were shown to catalyze the desired reaction. nocardia corallina (atcc 19070) exhibited complete substrate and product stereoselectivity as it reduced s-warfarin to the correspondi ...19827081986
a survey of the structures of mycolic acids in corynebacterium and related taxa.acid methanolysates of 104 strains of coryneform and related bacteria were examined for mycolic acids by thin-layer chromatography and mass spectrometry. the mycolic acids of corynebacterium sensu stricto strains were not uniform in size and structure, and considerable variation existed within the approximate limiting range of c22 to c36. two species, corynebacterium bovis and 'corynebacterium mycetoides', were particularly distinctive; the former had exceptionally low molecular weight mycolic a ...19827086391
a preferential isolation procedure for asporogenous gram-positive bacteria.a preferential isolation procedure was devised for asporogenous (asp), gram-positive (gp), aerobic or facultative anaerobic bacteria which included the genera arthrobacter, corynebacterium, brevibacterium, microbacterium, mycobacterium, and micrococcus (asp-gp bacteria). an antibiotics-mixture agar which contained 5 to 10 micrograms per ml of colistin, 10 to 20 micrograms per ml of nalidixic acid and 30 micrograms per ml of cycloheximide was used in the isolation. using this technique 47 asp-gp ...19827096199
microbiological examination of sebeel water.water samples from clay storage jugs ("zeers") located in homes and at public watering stands ("sebeels") at streets, mosques, and schools were examined. coliforms, fecal coliforms, and fecal streptococci were detected in 100, 69, 88, and 91.56% of the samples, respectively. the general microbiology of the water and some factors affecting microbial load were studied. the predominant bacterial genera of sebeel water were found to be staphylococcus. aerococcus, micrococcus, streptococcus, bacillus ...19827103483
structural requirements for neuraminidase induction in arthrobacter sialophilus.the effectiveness of 13 n-acetylneuraminic acid derivatives as potential inducers of arthrobacter sialophilus neuraminidase were examined. n-acetylneuraminic acid nitrogen and thioglycosides were not inducers, whereas 2,3-dehydro-n-acetylneuraminic acid, a transition state analog for neuraminidases, was the most effective inductive ligand. the c-4 hydroxyl function of n-acetylneuraminic acid was essential for enzyme derepression.19827107565
lack of ultraviolet mutagenesis in radiation-resistant bacteria.ultraviolet (uv) radiation did not induce rifampicin-resistant mutants in populations of the taxonomically-related radiation-resistant bacteria deinococcus radiodurans, d. radiopugnans, d. radiophilus and d. proteolyticus, although such mutants arose spontaneously at a low frequency and at a high frequency after treatment of cultures with n-nitroso compounds. the radiation-resistant bacteria arthrobacter radiotolerans and p-30-a were also uv-immutable whereas the more radiation-sensitive pseudom ...19827110166
chlorine resistance patterns of bacteria from two drinking water distribution systems.the relative chlorine sensitivities of bacteria isolated from chlorinated and unchlorinated drinking water distribution systems were compared by two independent methods. one method measured the toxic effect of free chlorine on bacteria, whereas the other measured the effect of combined chlorine. bacteria from the chlorinated system were more resistant to both the combined and free forms of chlorine than those from the unchlorinated system, suggesting that there may be selection for more chlorine ...19827149722
[arthrobacter globiformis - a new yeast-lysing bacterium].a bacterium capable to lyse viable yeast cells was isolated from compost enriched with baker's yeast cells and was identified as arthrobacter globiformis. the yeast lytic enzyme complex produced by shaking culture was precipitated with ammonium sulfate, dialyzed and lyophilized. the crude residue contained beta-glucanase and alpha-mannanase, yet not chitinase. optimale carbon sources in the culture medium for a high enzyme synthesis were 0.5% beta-glucan for the production of beta-glucanase, res ...19827157841
isolation and characterization of a pentachlorophenol-degrading bacterium.with a new enrichment protocol, pentachlorophenol (pcp)-degrading bacteria were isolated from soil, water, and sewage. when characterized, all isolates were related and shared characteristics of the genus arthrobacter. growth rates for strain nc were determined for a number of substrates, including pcp and 2,4,6-trichlorophenol. changes in pcp concentration affected growth rate and length of the lag phase but not cell yield. increasing the ph from 6.8 to 7.8 decreased the length of the lag phase ...19827159084
muscle glycolipids in inherited muscular dystrophy of chickens.gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. total glycolipid content of the degenerating muscles was higher than the normal muscles. in addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chickens. based on the thin-layer chromatographic mobility, treatment with neuraminidases from vibrio cholerae and arthrobacter ureafaciens, and reactivity of the asialo-derivative tow ...19827160480
lipids in the classification of nocardioides: reclassification of arthrobacter simplex (jensen) lochhead in the genus nocardioides (prauser) emend. o'donnell et al. as nocardioides simplex comb. nov.representative strains of nocardioides, arthrobacter simplex and arthrobacter tumescens were degraded by acid methanolysis and the fatty acid esters released examined by thin-layer and gas chromatography. branched-chain 14-methylpentadecanoic acid (iso-16) was the predominant component in all but one of the nocardioides strains. arthrobacter simplex also contained major amounts of this acid whereas a. tumescens had only minor amounts. all of the test strains possessed 15 and 17 carbon straight c ...19827171289
structural features of cytochalasins responsible for gram-positive bacterial inhibitions.a study of the relative effectiveness of some eighteen natural and synthetically modified cytochalasins on the uptake of glucose by the gram-positive bacterium arthrobacter sialophilus showed that cytochalasins b, c or d and aspochalasins a, c or d were inactive natural congeners. the presence of an alpha,beta-unsaturated carbonyl group in the macrolide moiety of these compounds with appropriate bioisosteric placement, as exemplified by cytochalasin a and aspochalasin b, are requisite molecular ...19827174519
distribution of beta-lactam and beta-lactone producing bacteria in nature.over one million bacteria were isolated from a large variety of soil, plant and water samples collected from different environments and examined in an extremely sensitive and highly specific screen for beta-lactam production. a group of seven related monocyclic beta-lactams (monobactams) were isolated from strains representing four genera-agrobacterium, chromobacterium, gluconobacter and pseudomonas. monobactam-producing strains of agrobacterium and pseudomonas were isolated only rarely. produci ...19827174535
isolation and characterisation of sterol metabolites formed by an arthrobacter species. 19827184854
nucleic acid hybridization studies on microbacterium, curtobacterium, agromyces and related taxa.thirty strains of agromyces, arthrobacter, curtobacterium, brevibacterium, corynebacterium and microbacterium, exhibiting the rare peptidoglycan of group b, were subjected to extensive nucleic acid hybridization studies. the dna homology values indicate that corynebacterium insidiosum dsm 20157 is genetically identical with corynebacterium michiganense dsm 20134. corynebacterium sepedonicum ncppb 378 and corynebacterium nebraskense dsm 20400 are closely related to corynebacterium michiganense ds ...19826183393
production of glutamic acid by an arthrobacter sp. i. identification and nutritional requirement in relation to glutamic acid production. 19826188337
microflora of antarctic krill (euphausia superba). 19826188340
production of surfactant by arthrobacter paraffineus atcc 19558.a. paraffineus atcc 19558 grown in mmsm (modified mineral salts medium) containing hydrocarbon produced surfactant, with a maximum cmc(-1) value obtained by using hexadecane as the carbon source. no activity of surface active agent in whole broth was observed when glucose was used in the mmsm instead of hexadecane. the biomass concentration obtained with glucose was about 40% of that obtained with hexadecane. glucose (4%) in the medium containing hexadecane caused a 27 and 21% decrease of biomas ...198218546108
possible role of coryneform bacteria in age gelation of ultrahigh-temperature-processed milk.two strains of psychrotrophic, gram-positive, proteolytic bacteria isolated from raw milk were identified as arthrobacter or closely related coryneform species. we inoculated raw-milk samples with the strains (one strain per sample) and compared rates of gelation after ultrahigh-temperature processing with that of ultrahigh-temperature-processed controls. the trial indicated that either organism could play a role in age gelation of ultrahigh-temperature-processed milk.198216346043
isolation of arthrobacter bacteriophage from soil.soil was percolated with water and various nutrient solutions, and then the percolates were analyzed for bacteriophages which produced plaques on various arthrobacter strains. the water percolates did not contain detectable phage. in contrast, phages for a. globiformis strains atcc 8010 and 4336, and for several recent arthrobacter species soil isolates, were easily detected in nutrient broth, soil extract, and cation-complete medium percolates. these percolates did not contain phage that produc ...198116345792
effect of microorganisms on the phytotoxicity of herbicides. vi. modification of venzar activity by bacterial cultures.interactions between two bacterial strains and venzar were compared. it was found that the mechanism of interactions is various and causes the modification of herbicide phytotoxicity. metabolites of bacillus subtilis 72 interfered with herbicide by affecting physiological processes in plant tissues and enhancing its inhibitory influence. arthrobacter sp. 18 strain decreased the phytoinhibitory effect of herbicide due to conjugation with the carrier from venzar.19816168180
a phylogenetic analysis of staphylococci, peptococcus saccharolyticus and micrococcus mucilaginosus.the intra- and intergeneric relationships of the genus staphylococcus, and the phylogenetic position of peptococcus saccharolyticus and micrococcus (staphylococcus salivarius), were investigated by comparative oligonucleotide cataloguing of 16s rrna. all the staphylococci investigated form a phylogenetically coherent group at the genus level that, in addition, contains the anaerobic species peptococcus saccharolyticus. the genus staphylococcus belongs to the broad bacillus-lactobacillus-streptoc ...19816172548
influence of methylheptenone and related phytoplankton norcarotenoids on heterotrophic aquatic bacteria.certain norcarotenoids, which have recently been found as excretion products of freshwater cyanobacteria and algae, are potent inhibitors of different metabolic functions in heterotrophic bacteria. 6-methylhept-5-en-2-one showed the strongest effects and acted as a noncompetitive inhibitor of both glucose uptake and respiration by aquatic isolates of chromobacterium lividum and arthrobacter sp. inhibition of the heterotrophic potential of glucose uptake by 6-methylhept-5-en-2-one was characteriz ...19817214229
inhibition of fusarium moniliforme var. subglutinans, the causal agent of pine pitch canker, by the soil bacterium arthrobacter sp.a species of arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. fusarium moniliforme var. subglutinans (fms). arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. soil samples from two seed orchards with pitch canker and one from a healthy pine plantation all yielded arthrobacter. these isolates were evaluated for their ability to inhibit the growth of fms isolates from pitch canker tissue and fr ...19817214230
[bacterial population dynamics in a soil--plant system].the dynamics of rhizobium leguminosarum and arthrobacter crystallopoietes populations introduced into soil at different levels of density was studied in a zone near barley roots. microbial life with a high rate of growth was found only at the root surface. for a. crystallopoietes, the ultimate bacterial incidence at the root surface was found to depend on the original level of population density. for rh. leguminosarum, the ultimate incidence was shown to reach an identical level irrespective of ...19817219211
[utilization of 4-chlorobenzoic acid by arthrobacter globiformis].a strain of arthrobacter globiformis utilizing 4-chlorobenzoic acid as a sole source of carbon and energy was isolated by the method of enrichment cultures from vegetable garden soil near moscow. the yield of released chlorine upon the utilization of 4-chlorobenzoic acid exceeded 96% of the theoretically possible one. biotin stimulated noticeably the utilization of the acid. the concentration of 4-chlorobenzoic acid that apparently did not inhibit the growth of the isolated organism was within t ...19817219218
arthrobacter p1, a fast growing versatile methylotroph with amine oxidase as a key enzyme in the metabolism of methylated amines.a facultative methylotrophic bacterium was isolated from enrichment cultures containing methylamine as the sole carbon source. it was tentatively identified as an arthrobacter species. extracts of cells grown on methylamine or ethylamine contained high levels of amine oxidase (e.c. 1.4.3) activity. glucose- or choline-grown cells lacked this enzyme. oxidation of primary amines by the enzyme resulted in the formation of h2o2; as a consequence high levels of catalase were present in methylamine- a ...19817224781
[microbial transformation of 16 beta-methyl-5 alpha-delta 9(11)-pregnene-3 beta, 17 alpha, 21-triol-20-one-3 beta, 21-diacetate and 17 alpha-methyl-17 beta-hydroxy-5 alpha-androstan-3-one (author's transl)]. 19817246181
the effect of phytohormones produced by arthrobacter sp. on the phosphatase activity in plant roots.phytohormonal activity (auxins, gibberellins, cytokinins) was tested in the supernatant of a culture of arthrobacter sp. crude extract of the phytohormonal fraction was used as substrate for the growth of lactuca sativa seedlings. treating with bacterial hormones resulted in an increased plant develop- ment. furthermore, a sharp increase of the acid phosphatase activity was observed in the roots.19817262713
bacterial degradation of 3,4,5-trimethoxyphenylacetic and 3-ketoglutaric acids.when grown at the expense of 3,4,5-trimethoxyphenylacetic acid, a species of arthrobacter readily oxidized 3,4-dihydroxy-5-methoxyphenylacetic acid, but other structurally related aromatic acids were oxidized only slowly. cell extracts contained a dioxygenase for 3,4-dihydroxy-5-methoxyphenylacetate, and the corresponding trihydroxy acid, which was not attacked by the enzyme, inhibited oxidation of this ring-fission substrate. cell suspensions did not release carbon dioxide from 3,4-[methoxyl-14 ...19817263613
2,3-dehydro-4-epi-n-acetylneuraminic acid; a neuraminidase inhibitor.treatment of n-acetylneuraminic acid methyl ester with sulfuric acid and acetic anhydride at 50 degrees followed by deacetylation gave 2,3-dehydro-2-deoxy-n-acetylneuraminic acid methyl ester and methyl 5-acetamido-2,6-anhydro-2,3,5-trideoxy-d-glycero-d-talo-non-2-enonate (2,3-dehydro-4-epi-neuac methyl ester) in equal yields (approximately 40% each). the structure of the latter was ascertained primarily from analysis of its mass spectrum and 1h- and 13c-nuclear magnetic resonance spectra. the r ...19817273035
[3-oxosteroid-delta 1-dehydrogenase localization in mycobacterium rubrum and arthobacter globiformis cells].osmotically susceptible forms were obtained from mycobacterium rubrum and arthrobacter globiformis (mycobacterium globiforme) cells. the activity of 3-oxosteroid-delta 1-dehydrogenase was comparatively estimated in subcellular fractions after differential centrifugation of cell homogenates prepared either mechanically or by lysis of osmotically susceptible forms. the results indicate that the enzyme is located in the cells of the above microorganisms in both the free state (in the fraction of so ...19817311905
the release of n-acetyl- and n-glycolloyl-neuraminic acid from soluble complex carbohydrates and erythrocytes by bacterial, viral and mammalian sialidases.a series of substrates, sialyl(2 leads to 6)galnac and ganglioside gm3, containing either n-acetylneuraminic acid (acneu) or n-glycolloylneuraminic acid (gcneu), has been prepared. the trisaccharide gcneu(2 leads to 3)lactose was preapred by ozonolysis of gcneu-gm3, and the disaccharides acneu(2 leads to 6)galnac and gcneu(2 leads to 6)galnac were isolated from bovine submandibular-gland mucin by alkali elimination. sialidases from newcastle-disease virus, fowl-plague virus, influenza virus a2, ...19817325957
enzymic formation of difructose anhydride iv from bacterial levan.a novel enzyme for producing difructose anhydride iv (di-d-fructofuranose 2,6' : 6,2' dianhydride) from levan of bacillus mesentericus was prepared from a culture of arthrobacter ureafaciens, and the conditions and method to determine the enzymatic activity were determined. levan of bacillus subtilis also yielded the difructose dianhydride, but inulin, inulobiose, sucrose, levanbiose, and levantriose did not produce it on enzymic reaction under similar test conditions.19817338521
[glycomacropeptide from whey: a substrate for determining the activity of bacterial neuraminidases]. 19817345910
a new enzymatic method for the determination of sialic acid in serum and its application for a marker of acute phase reactants. 19817021999
microbial adaptations to stress: some lessons to be learned from aerobes. 19817023462
an efficient technique for the isolation of yeast spores and the preparation of spheroplast lysates active in protein synthesis. 19817032354
scope and mechanism of carbohydrase action. stereocomplementary hydrolytic and glucosyl-transferring actions of glucoamylase and glucodextranase with alpha- and beta-d-glucosyl fluoride.rhizopus niveus glucoamylase and arthrobacter globiformis glucodextranase, which catalyze the hydrolysis of starch and dextrans, respectively, to form d-glucose of inverted (beta) configuration, were found to convert both alpha- and beta-d-glucosyl fluoride to beta-d-glucose and hydrogen fluoride. each enzyme directly hydrolyzes alpha-d-glucosyl fluoride but utilizes th beta-anomer in reactions that require 2 molecules of substrate and yield glucosyl transfer products which are then rapidly hydr ...19816787047
production and isolation of siderophores from the soil fungus epicoccum purpurascens.a large number of iron transport agents, siderophores, which stimulated the growth of arthrobacter flavescens jg-9, were isolated during a study of the antitumor activity associated with the metabolic products of the fungus epicoccum purpurascens. the production of the siderophores was significantly enhanced in a variety of media by culture of the fungus in the near absence of ferric iron. a novel method of purification involving a carboxylic ion-exchange resin separated the siderophores into fo ...19816453608
siderophore production by pathogenic neisseria spp.previous studies have established the importance of iron acquisition to the growth and virulence of neisseria meningitidis and neisseria gonorrhoeae. although preliminary evidence that the neisseria spp. produce siderophores has been presented, the exact mechanism of iron acquisition has remained obscure. siderophore production by n. gonorrhoeae and n. meningitidis was induced in two different low-iron media. the iron-reactive siderophores, "gonobactin" and "meningobactin," were partially purifi ...19816454659
isolation and structural characterization of human lymphocyte and neutrophil gangliosides.gangliosides were isolated from purified preparations of human peripheral blood lymphocytes and neutrophils. structural analyses and comparisons were performed by direct probe mass spectrometry and by degradation studies with the following enzymes: escherichia freundii endo-beta-galactosidase; clostridium perfringens and arthrobacter ureafaciens neuraminidase; and jack bean beta-n-acetylhexosaminidase and beta-galactosidase. this combination of techniques allowed us to obtain carbohydrate compos ...19816780555
[dna-methylase from arthrobacter luteus screens dna from the action of site-specific endonuclease alu i].dna-methylase was isolated from a cell extract of a. luteus and partially purified by chromatography on phosphocellulose. the purified enzyme methylates dna of phage lambda and plasmids pbr322, thus making them resistant to a subsequent action of endonuclease alu i. it has been shown that cytosine is the object of methylation within dna. this modification does not screen dna from the action of site-specific endonucleases sal i, bam hi, ecor i, ecor ii, xho i and xho ii. it has been experimentall ...19816268201
[1h-nmr spectroscopy--a potent method for the determination of substrate specificity of sialidases (author's transl)].we describe here the application of 1h-nmr spectroscopy to determine the substrate specificity of sialidases using a 1:1 mixture of neuac alpha 2-3gal beta 1-4glc and neuac alpha 2-6gal beta 1-4glc, one viral and five bacterial sialidases. this method utilizes the separate signals in nmr spectra, characteristic for the different alpha ketosidically linked neuac residues and also for bound and free neuac. the signals generally most suitable for these purposes are those of h3a, h3e and ncoch3. by ...19816273284
the synthesis of 4-methylumbelliferyl alpha-ketoside of n-acetylneuraminic acid and its use in a fluorometric assay for neuraminidase. 19806243885
[1h-nmr-spectroscopic evidence for the release of n-acetyl-alpha-d-neuraminic acid as the first product of neuraminidase action (author's transl)].the 1h-nmr spectroscopy was used to study the anomeric configuration of n-acetyl-d-neuraminic acid released by the action of neuraminidase. the hydrolysis of neuacalpha 2 leads to 3 gal-beta 1 leads to 4glc (20mm) by the enzymes of clostridium perfringens and arthrobacter ureafaciens (50 mu, 150 mu and 800 mu, respectively) in 50mm na/k-phosphate buffer pd 5.4 was observed by recording the spectra. on the basis of the characteristic signals of the protons at c-3 (alphaneuac: delta[h(3e)] = 2.72, ...19806253376
myceloid growth of arthrobacter globiformis and other arthrobacter species.transitory myceloid growth occurs in certain complex media with arthrobacter globiformis strain atcc 8010. this type of growth, however, was not observed in a medium which contained an array of metal ions but did not contain agents able to complex metal ions. addition of metal-complexing agents to this medium caused an interruption in the life cycle of strain 8010 so that growth occurred only as the myceloid form. it appeared that manganese was the critical metal that was removed by the metal-co ...19806254945
a comparison of the cytochrome complements of seven strains of marine manganese-oxidizing bacteria. 19806259842
[change in the microbiol complexes of soddy podzol soil under the influence of long-term spring wheat monoculture].the special composition and the properties of microorganisms predominant in the soil and in the rhizosphere of summer wheat were studied in the conditions of monoculture and corp rotation. noticeable differences were found in the composition of microbial complexes: microorganisms belonging to the genus arthrobacter prevailed in the conditions of corp rotation, whereas cultures of the genera pseudomonas and bacillus predominated in the conditions of monoculture. many of the latter possessed weak ...19806451787
[ratio of coryneform bacteria to the organic substance concentration].the effect of various organic substances and their concentrations on growth was studied with 10 strains of coryneform bacteria belonging to different taxonomic groups. the dynamics of the cultural growth depended on the nature of a substrate, glucose or acetate. arthrobacter globiformis 281, a. variabilis 289, nocardia erythropolis 236, n. globerula 502, n. minima 311 and n. rhodochrous 435 had a higher growth rate on acetate than on glucose. a. pascens 284, brevibacterium ammoniagenes 334, cory ...19806782432
cell wall composition in the classification and identification of coryneform bacteria. 19806792712
a taxonomic study of gardnerella vaginalis (haemophilus vaginalis) gardner and dukes 1955.fifty-five strains received as haemophilus vaginalis or as catalase-negative coryneform bacteria from the vagina together with 61 marker cultures were subjected to numerical phenetic analyses using 149 unit characters. the data were examined using the simple matching (ssm), jaccard (sj) and pattern (dp) coefficients and clustering was achieved using the average linkage algorithm. cluster composition was not markedly affected by the coefficient used or by test error, estimated at 6 . 5%. the h. v ...19806971916
cell-free synthesis of a flavoprotein containing the 8 alpha-(n3-histidyl)-riboflavin linkage.6-hydroxy-d-nicotine oxidase is an inducible flavorprotein of arthrobacter oxidans in which one mole of fad is bound covalently to the polypeptide chain. during cell-free translation of polysomes from nicotine-induced a. oxidans cells in the presence of an escherichia coli (mre 600) supernatant fraction, labelled fad, leucine and histidine were incorporated into 6-hydroxy-d-nicotine oxidase in the same ratio found in the enzyme isolated from whole cells. this indicates that one mole fad is coval ...19806988214
evolutionary relationship between halobacterium cutirubrum and eukaryotes determined by use of aminoacyl-trna synthetases as phylogenetic probes.the cross-species reactivities between trnas and aminoacyl-trna synthetases have been employed as a basis to estimate the relatedness of various prokaryotes to the eukaryotes. the trna of halobacterium cutirubrum, unlike that of other prokaryotes tested, including agrobacterium tumefaciens, arthrobacter luteus, bacillus subtilis, bacillus stearothermophilus, escherichia coli, micrococcus luteus, myxococcus xanthus, rhodopseudomonas spheroides, and thermus aquaticus, was found to share with yeast ...19806989454
[superoxide dismutase--radiobiologic significance and possibilities (review)].modern data are considered on the importance of superoxide radical in the oxygen effect of ionizing radiation as well as the participation of superoxide dismutase in biological radioresistance. biological role and some physico-chemical properties of the enzyme are described. estimation of the superoxide dismutase activity might serve as a test for study of the primary effect of ionizing radiation on cells; at the same time, the enzymatic preparations could be used as radioprotective drugs.19807006203
a critical analysis of kinetic data of 3-hexulosephosphate synthases. michaelis-menten or complex characteristics.investigations of the 3-hexulosephosphate synthase (hps) from different methylotrophic bacteria have revealed apparent discrepancies in kinetic behaviour. in all methanol-utilizing species investigated by us the kinetic characteristics showed intermediary plateau regions. therefore, this behaviour is assumed to be a general feature of the hps from all non-methane-utilizing methylotrophic bacteria. however, this assumption is in contrast to the results of other authors. both for methylomonas m15 ...19806775423
[sulfur and iron cycling bacteria in low-sulfate meromictic lake kuznechikha].the hydrochemical characteristics, the composition of species and the localization of bacterial species involved in oxidation of sulfide and ferrous salts were studied in the meromictic lake kuznechikha with a low sulfate content in summer and in winter. the rate of bacterial sulfate reduction and the rates of bacterial and algal photosynthesis were determined using a radioisotope technique. the meromictic nature of the lake is due to the accumulation of ferrous salts (up to 212 mg/l fe2+) in th ...19806777648
radioimmunoassay of arthrobacter neuraminidase: applications to catalytic and taxonomic studies. 19806779664
[hydrocortisone transformation into prednisolone by microorganisms]. 19807041529
[arthrobacter simplex var. amylolyticus var. nov., a producer of a hexaene antibiotic in the fradicin-mycelin group]. 19807356309
malonichrome, a new iron chelate from fusarium roseum.the predominant iron chelates, or siderochromes, produced by the fungus, fusarium roseum during culture periods up to seven days are the ester type fusarinine compounds. during longer periods of incubation, the fusarinine compounds completely disappear from the culture medium and are replaced by a new siderochrome. the new compound has been isolated, purified, and its structure determined. it is a cyclic hexapeptide containing one residue of l-alanine, two residues of glycine and three residues ...19807388041
bioconversion of pregnenolone to 1,4 androstadiene 3,17 dione by arthrobacter simplex. 19807390551
[triglycerides of saprophytic mycobacteria].triglycerides produced by mycobacteria and arthrobacteria grown in media with hexadecane or glucose were studied by mass spectrometry. in all of the cases, triglycerides were mixtures of homologues. the triglycerides of arthrobacter ceroformans grown in a medium with hexadecane contained 90% of tripalmitate and 10% of a mixed triglyceride comprising two residues of palmitic acid and one residue of palmitoleinic acid. mixed triglycerides containing one unsaturated acyl group prevailed in mycobact ...19807393006
[electrokinetic properties of arthobacter siderocapsulatus].the electro-kinetic properties of arthrobacter siderocapsulatus were determined using microelectrophoresis in alternating electric field. the surface of bacterial cells was shown to bear positive charge within a wide range of ph. the positive charge of the cells should be attributed apparently to the presence of mucous capsules containing manganese dioxide since the growth of the bacterium is known to involve oxidation of manganese or ferrous iron and accumulation of their hydroxides in the caps ...19807402120
degradation of (-)-ephedrine by pseudomonas putida. detection of (-)-ephedrine: nad+-oxidoreductase from arthrobacter globiformis.a bacterium utilizing the alkaloid (-)-ephedrine as its sole source of carbon was isolated by an enrichment-culture technique from soil supplemented with 4-benzoyl-1,3-oxazolidinon-(2). the bacterium was indentified as pseudomonas putida by morphological and physiological studies. the following metabolites were isolated from the culture fluid: methylamine, formaldehyde, methylbenzoylcarbinol (2-hydroxy-1-oxo-1 phenylpropane), benzoid acid, pyrocatechol and cis, cis-muconic acid. a pathway for th ...19807405363
purification, properties and formation of arginine-alpha-ketoglutarate transaminase in arthrobacter simplex.arginine-alpha-ketoglutarate transaminase was purified 460-fold with 1.4% yield from arthrobacter simplex grown on arginine as a carbon source. the preparation was more than 90% pure on polyacrylamide gel electrophoresis, and the molecular weight of the enzyme was calculated to be 110 000. the enzyme exhibited absorption maxima at 280, 330 and 370 nm. the 370 nm peak decreased with increase in the 330 nm peak on addition of arginine km values for arginine, alpha-ketoglutarate, glutamate and alph ...19807426667
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