Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| metabolism of endogenous trehalose by streptomyces griseus spores and by spores or cells of other actinomycetes. | the disaccharide trehalose is accumulated as a storage product by spores of streptomyces griseus. nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. in contrast, spores rapidly depleted their trehalose pools during the first hours of germination. extracts of dormant spores contained a high specific activity of the enzyme trehalase. the level of trehalase remained relatively constant during germination or incubation in buffer. nongerminating sp ... | 1987 | 3117770 |
| covalent flavinylation of 6-hydroxy-d-nicotine oxidase analyzed by partial deletions of the gene. | the expression of the enzymatically active 6-hydroxy-d-nicotine oxidase (6-hdno) from arthrobacter oxidans requires the covalent attachment of fad to the polypeptide chain. how this modification takes place and at what time during the synthesis of the polypeptide is not known. we investigated the possibility of cotranslational flavinylation by generating various deletions of the 6-hdno gene carried on appropriate plasmid vectors. the polypeptides expressed from these plasmids were analyzed for t ... | 1987 | 3036509 |
| immobilization of living microbial cells in polyacrylamide gel. | 1987 | 3298943 | |
| integration of bioconversions and downstream processing. some model studies. | 1987 | 3124693 | |
| transformation of arthrobacter and studies on the transcription of the arthrobacter erma gene in streptomyces lividans and escherichia coli. | we report the development of a plasmid-mediated transformation system for arthrobacter sp. nrrlb3381, using the streptomyces cloning vector pij702. our procedure gives a transformation frequency of 10(3)/micrograms of plasmid dna. in addition we have explored the expression of the arthrobacter erma gene in streptomyces lividans and escherichia coli, and shown that the erma promoter is recognized in s. lividans not e. coli. the relationship between arthrobacter, streptomyces and e. coli promoters ... | 1987 | 2443127 |
| [microbial transformation of 16 alpha-methyl-3 beta, 17 alpha-dihydroxy-5 alpha-pregnane-20-one-3 acetate]. | 1987 | 3618239 | |
| [kinetics of delta'-dehydrogenation of hydrocortisone by arthrobacter simplex by-2-13. i. kinetics of delta'-dehydrogenation of hydrocortisone by free cells of arthrobacter simplex by-2-13]. | 1987 | 3630138 | |
| [microbial reduction of c20-keto of corticosteroids by arthrobacter 9-2. ii. bioconversion of 3 beta,17 alpha,21-triol-5 alpha-pregnan-3-one]. | 1987 | 3630139 | |
| microbiological degradation of malodorous substances of swine waste under aerobic conditions. | phenol, p-cresol, and volatile fatty acids (vfa; acetic, propionic, isobutyric, butyric, isovaleric, and valeric acids) were used as odor indicators of swine waste. aeration of the waste allowed the indigenous microorganisms to grow and degrade these malodorous substances. the time required for degradation of these substances varied according to the waste used, and it was not necessarily related to their concentrations. using a minimal medium which contained one of the malodorous compounds as so ... | 1987 | 16347254 |
| uptake of glyphosate by an arthrobacter sp. | the uptake of glyphosate (n-[phosphonomethyl]glycine) by an arthrobacter sp. which can utilize this herbicide as its sole source of phosphorus was investigated. orthophosphate suppressed the expression of the uptake system for glyphosate and also competed with glyphosate for uptake. the k(m) for glyphosate uptake was 125 mum, and the k(i) for orthophosphate was 24 mum. organophosphonates as well as organophosphates inhibited glyphosate uptake, but only organophosphates and orthophosphate suppres ... | 1987 | 16347356 |
| purification and characterization of benzonitrilases from arthrobacter sp. strain j-1. | we found two kinds of benzonitrilases, designated benzonitrilases a and b, in a cell extract of arthrobacter sp. strain j-1 grown on benzonitrile as a sole carbon and nitrogen source. benzonitrilases a and b were purified approximately 409-fold and 38-fold, respectively. purified benzonitrilase a appeared to be homogeneous according to the criteria of polyacrylamide gel electrophoresis. both the enzymes hydrolyzed benzonitrile to benzoic acid and ammonia without forming benzamide as an intermedi ... | 1986 | 16346987 |
| identification of eubacteria isolated from leaf cavities of four species of the n-fixing azolla fern as arthrobacter conn and dimmick. | the nutritional and physiological characteristics of 15 isolates from four species of the azolla fern were determined. although some minor variation existed in levels of urease activity, ability to utilize xylose, and formation of n(2) gas from no(3), all 15 isolates were rather similar and believed to represent a single species. these eubacteria exhibited aminopeptidase activity and became viscous when treated with koh, similar to gram-negative organisms; however, the absence of lipopolysacchar ... | 1986 | 16347144 |
| acetylene metabolism and stimulation of denitrification in an agricultural soil. | the effects of c(2)h(2) metabolism on n(2)o production were examined in soil slurries. enrichment of c(2)h(2) consumption activity occurred only in aerobic incubations. rapid disappearance of subsequent c(2)h(2) additions, stimulation of co(2) production, and most-probable-number enumerations of c(2)h(2) utilizers indicated enrichment of the population responsible. during c(2)h(2) consumption in slurries incubated statically under air, maximal rates of n(2)o evolution were 19 times higher than t ... | 1986 | 16347172 |
| bacterial growth rates and competition affect nodulation and root colonization by rhizobium meliloti. | the addition of streptomycin to nonsterile soil suppressed the numbers of bacterial cells in the rhizosphere of alfalfa (medicago sativa l.) for several days, resulted in the enhanced growth of a streptomycin-resistant strain of rhizobium meliloti, and increased the numbers of nodules on the alfalfa roots. a bacterial mixture inoculated into sterile soil inhibited the colonization of alfalfa roots by r. meliloti, caused a diminution in the number of nodules, and reduced plant growth. enterobacte ... | 1986 | 16347173 |
| microbial life at extremely low nutrient levels. | many microorganisms ("oligotrophs") grow in distilled water: pseudomonas spp., caulobacter spp., hyphomicrobium spp., arthrobacter spp., seliberia spp., bactoderma alba, corynebacterium spp., amycolata (nocardia) autotrophica, mycobacterium spp., yeasts, and chlorella spp. also, certain lower fungi can be found here. in the laboratory, these organisms thrive on contaminations of the air (co, hydrocarbons, h2, alcohols etc.). all are euryosmotic and often grow also in higher concentrations of ... | 1986 | 11537832 |
| pilot-plant production of prednisolone using calcium alginate immobilized arthrobacter simplex. | the maximum conversion of hydrocortisone suspensions at initial substrate concentrations greater than 4 g/l by immobilized arthrobacter simplex in a batch reactor was 80-85%. by feeding hydrocortisone suspensions continuously to either a fed-batch-operated stirred tank reactor or to a continuous-flow airlift loop reactor, at a rate such that the soluble hydrocortisone concentration in the reactor remained ca. 0.05 g/l, 95% conversion of substratewas obtained at final product concentrations excee ... | 1986 | 18561228 |
| coimmobilization of substrate and biocatalyst: a method for bioconversion of poorly soluble substances in water milieu. | coimmobilization of biocatalyst and substrate was studied as a method to increase the conversion rate in systems with substrates of extremely low solubility in water. the system studied was the conversion of hydrocortisone to prednisolone by arthrobacter simplex. as a matrix for coimmobilization, alginate turned out to be superior to agar and agarose. after the reaction was complete, the beads were solubilized, andthe cells recovered for reuse, by centrifugation, whereas the prednisolone was ext ... | 1986 | 18561232 |
| on the influence of different growth conditions to the resistance of some methylotrophic bacteria to aldehydes. | the resistance to formaldehyde of several facultatively methylotrophic bacteria has been investigated. the mic-values were in the range of formaldehyde concentrations used for preservation purposes. the resistance could be explained partly by the action of aldehyde dehydrogenase found in two of the strains and by the development of a penetration barrier by the cell envelopes described formerly. | 1986 | 3092505 |
| [esterase activity of hydrocarbon-oxidizing bacteria]. | the activity of esterase was studied in bacteria oxidizing hydrocarbons and belonging to the genera rhodococcus, arthrobacter and pseudomonas. indophenyl acetate was used as a substrate of the reaction catalysed by the enzyme. exocellular esterases were not found. endocellular esterases differed in their activity and thermostability both among the genera and among species of one and the same genus. | 1986 | 3102911 |
| cloning and expression in escherichia coli of the gene for an arthrobacter beta-(1----3)-glucanase. | when inserted in the correct orientation at the bamhi site of plasmid yrp7, an 8.6-kilobase bamhi fragment of arthrobacter sp. strain ycwd3 dna gave escherichia coli hb101 cells harboring the recombinant plasmid pbx20 the ability to lyse bakers' yeast cell walls or bakers' yeast glucan in agar medium. an extract of the transformed e. coli cells contained an endo-beta-(1----3)-glucanase with the same activity pattern as that of glucanase i produced by arthrobacter sp. strain ycwd3. although part ... | 1986 | 3096974 |
| plasmid pao1 of arthrobacter oxidans encodes 6-hydroxy-d-nicotine oxidase: cloning and expression of the gene in escherichia coli. | the 160 kb plasmid pao1 from arthrobacter oxidans (brandsch and decker 1984) was subcloned in escherichia coli with the aid of the plasmid vectors pur222 and pbr322. screening of the recombinant clones for enzyme activity revealed that the flavoenzyme 6-hydroxy-d-nicotine oxidase (6-hdno), one of the enzymes of the nicotine-degradative pathway in a. oxidans, is encoded on pao1. immunoprecipitation of 35s-methionine-labelled e. coli cells with 6-hdno-specific antiserum and expression of recombina ... | 1986 | 3007941 |
| [the use of bioelectrochemical systems in steroid biosynthesis]. | the feasibility of electrosynthesis involving the use of biocatalysts has been demonstrated in a model reaction of hydrocortisone oxidation to prednisolone catalyzed by arthrobacter globiformis cells. electrochemical regeneration of phenazine methosulfate, an artificial electron acceptor, was performed, using electrodes made of different materials. transformation products were analyzed by high performance liquid chromatography. | 1986 | 3768442 |
| sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid. | an immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. after chromatography of gangliosides, the plate was treated with arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. for the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. the resulting asialo-glycolipids, ga2 and ga1 were reacted first with a ... | 1986 | 3511969 |
| enzymes of vitamin b6 degradation. purification and properties of pyridoxine 5'-dehydrogenase (oxidase). | isolation and identification of a soil bacterium, arthrobacter cr-7, that grows with pyridoxine as a sole source of carbon and nitrogen are described. an inducible pyridoxine 5'-dehydrogenase (oxidase) (ec 1.1.99.9) that catalyzes conversion of pyridoxine to isopyridoxal, pyridoxine + x----isopyridoxal + xh2, the first step in utilization of pyridoxine as a growth substrate by this organism, was purified about 520-fold to homogeneity. the enzyme (mr = 112,000) is a dimer of probably identical su ... | 1986 | 3533935 |
| [microbial transformation of 16 alpha-methyl-5 alpha-delta 9(11)- pregnene-3 beta, 17 alpha, 21-triol-20-one-3 beta, 21-diacetate]. | 1986 | 3577788 | |
| evidence for the functional role of monosialoganglioside gm1 in synaptic transmission in the rat hippocampus. | the hippocampal slices were incubated with compounds which hydrolyze, modify or bind with sialic acid containing molecules. the efficiency of synaptic transmission was tested in the presence of these compounds. the size of the evoked extracellularly recorded potential following schaffer collateral stimulation was used as an indicator of synaptic transmission efficiency. sodium periodate (10 mm) and sodium perchlorate (59.2 mm) evoked a reversible (after washout) decrease in the size of the popul ... | 1986 | 3008944 |
| methylamine oxidase from arthrobacter p1. a bacterial copper-quinoprotein amine oxidase. | methylamine oxidase from arthrobacter p1 was purified to homogeneity. the enzyme oxidizes primary amines but not tyramine or polyamines like spermine and putrescine. the enzyme activity has a ph optimum of 8.0 with methylamine, and is inhibited by certain cations as well as anions at rather low concentrations. the enzyme has an mr of 167900, an isoelectric point of 4.6, consists of two (probably identical) subunits (mr 82250) and contains two copper atoms but no sugar residues. the visible absor ... | 1986 | 3780750 |
| purification and properties of synephrinase from arthrobacter synephrinum. | synephrinase, an enzyme catalyzing the conversion of (-)-synephrine into p-hydroxyphenylacetaldehyde and methylamine, was purified to apparent homogeneity from the cell-free extracts of arthrobacter synephrinum grown on (+/-)-synephrine as the sole source of carbon and nitrogen. a 40-fold purification was sufficient to produce synephrinase that is apparently homogeneous as judged by native polyacrylamide gel electrophoresis and has a specific activity of 1.8 mumol product formed/min/mg protein. ... | 1986 | 3729420 |
| surface localization of sialic acid on actinomyces viscosus. | this study reports the presence of sialic acid in actinomyces viscosus strains t14v and t14av. mild acid hydrolysis of whole organisms released a compound which reacted positively in the periodate-thiobarbituric acid, direct ehrlich's and resorcinol assays, and which co-chromatographed on paper with authentic n-acetylneuraminic acid. strain t14v contained 10-fold greater concentrations of sialic acid than did strain t14av. sialic acid content was dependent upon the stage of growth of the culture ... | 1986 | 3655719 |
| initial reactions of xanthone biodegradation by an arthrobacter sp. | this study examined the catabolism of xanthone by an arthrobacter sp. (strain gfb100) capable of growth on xanthone as its main source of carbon and energy. an early catabolic intermediate was 3,4-dihydroxyxanthone. this compound was isolated from the growth medium of a mutant strain of the arthrobacter sp. which lacked the xanthone-inducible dihydroxyxanthone ring-fission dioxygenase of the wild-type strain. cell extracts from wild-type xanthone-grown cells oxidized 3,4-dihydroxyxanthone to a y ... | 1986 | 3745120 |
| enzymes of vitamin b6 degradation. purification and properties of 4- and 5-pyridoxolactonases. | 4-pyridoxolactone and 5-pyridoxolactone, formed by dehydrogenation of pyridoxal or isopyridoxal during the bacterial degradation of vitamin b6 by pseudomonas ma-1 and arthrobacter cr-7, respectively, are hydrolyzed to the corresponding acids by distinct inducible lactonases which were purified to homogeneity. 4-pyridoxolactonase from pseudomonas ma-1 has an mr of 54,000 and contains two probably identical subunits of mr = 28,600. it has a ph optimum of 7.0, a km of 5.9 microm, and a vmax at 25 d ... | 1986 | 3771565 |
| enzymes of vitamin b6 degradation. purification and properties of isopyridoxal dehydrogenase and 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid dehydrogenase. | two nad+-dependent, highly specific pyridine-5-aldehyde dehydrogenases, 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid (compound 1) dehydrogenase and isopyridoxal dehydrogenase, were purified to homogeneity from pseudomonas ma-1 and arthrobacter cr-7, respectively. both enzymes are induced in response to growth of the organisms on pyridoxine and catalyze steps in the degradation of this compound by these organisms. compound 1 dehydrogenase (mr = 65,000) contains two subunits of equal size ... | 1986 | 3533936 |
| enzymes of vitamin b6 degradation. purification and properties of 5-pyridoxic-acid oxygenase from arthrobacter sp. | 5-pyridoxic-acid oxygenase, a cytoplasmic enzyme formed when arthrobacter cr-7 is grown with pyridoxine as a sole source of carbon and nitrogen, was purified about 190-fold to homogeneity from fully induced cells. the enzyme catalyzes reaction a, (formula: see text) the essential ring-opening step in the degradation of pyridoxine, and provides a second example of an fad-dependent oxygenase that adds both two hydrogen and two oxygen atoms to its substrate. 5-pyridoxic-acid oxygenase has an isoele ... | 1986 | 3771566 |
| studies in vitro on the flavinylation of 6-hydroxy-d-nicotine oxidase. | the gene of 6-hydroxy-d-nicotine oxidase (6-hdno), a flavoenzyme from arthrobacter oxidans with covalently bound fad, was expressed with the aid of an expression vector in a cell-free coupled transcription-translation system derived from escherichia coli mz9. ultraviolet irradiation of the e. coli extract did not affect synthesis of the 6-hdno polypeptide nor total protein synthesis but enzymatic 6-hdno activity could not be detected. addition of fad to the irradiated cell extract restored the c ... | 1986 | 3533536 |
| biochemical properties of n-methylamides of sialic acids in gangliosides. | a simple and rapid method for the preparation of n-methylamides ( - conhch3) of sialic acids in gangliosides and biochemical properties of the modified gangliosides are described. the sialic acid carboxyl groups of gangliosides were esterified with ch3i-dimethylsulfoxide, followed by heating with monomethylamine. the modified gangliosides were chemically identified by tlc, ir spectroscopy, glc-mass spectrometry and nmr spectroscopy. the n-methylamide derivative of gm1 produced a high titer igg a ... | 1986 | 2420784 |
| an erythromycin-resistance gene from an erythromycin-producing strain of arthrobacter sp. | a gene (erma) coding for a presumed erythromycin-resistance (err) determinant from an er-producing arthrobacter sp. strain (nrrlb3381) was isolated from a gene bank in phage vector lambda 2001 by probing with a streptomyces err gene. strongly hybridizing fragments were subcloned and the appropriate segments sequenced. the erma gene is 76 mol% g + c in content and specifies a protein of 340 aa with an mr of 37454. s1 nuclease mapping and primer extension identified the putative promoter, which re ... | 1985 | 4043733 |
| immunodiffusion and lipid analyses in the classification of "mycobacterium album" and the "aurantiaca" taxon. | comparative immunodiffusion studies were performed on representative strains of "mycobacterium album", the "aurantiaca" taxon and an "aurantiaca"-like group of actinomycetes, using reference systems representing 25 taxa assigned to the genera arthrobacter, corynebacterium, kurthia, mycobacterium, nocardia, rhodococcus, streptomyces and the "aurantiaca" taxon. thin-layer chromatographic analyses of whole-organism methanolysates were carried out on single strains of "mycobacterium album" and the " ... | 1985 | 3923731 |
| growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin b. | vibrio alginolyticus, streptomyces griseus, arthrobacter g12, bacillus sp. and cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. arthrobacter g12, bacillus sp. and cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. all bacteria produced extracellular proteases and all bacteria except arthrobacter g12 produced extracellular chitinases. growth of cytophaga sp. on colloidal chitin was para ... | 1985 | 3929028 |
| in vivo and in vitro expression of the 6-hydroxy-d-nicotine oxidase gene of arthrobacter oxidans, cloned into escherichia coli, as an enzymatically active, covalently flavinylated polypeptide. | the 6-hydroxy-d-nicotine oxidase gene of arthrobacter oxidans was cloned into e.coli with the aid of the expression vector pkk223-3. this enzyme, as well as the e.coli enzymes succinate dehydrogenase and fumarate reductase, bears the cofactor fad covalently attached to the polypeptide through a his-n3-8 alpha-linkage. the amino acid sequence surrounding the histidine residue involved in fad binding in 6-hydroxy-d-nicotine oxidase and the two e.coli enzymes, however, show no homology. nevertheles ... | 1985 | 3905431 |
| glutathione-independent isomerization of maleylpyruvate by bacillus megaterium and other gram-positive bacteria. | maleylpyruvate, the ring fission product of gentisic acid, was found to be isomerized to fumarylpyruvate without a requirement for glutathione by an enzyme activity found in cell extracts of m-hydroxybenzoate-grown bacillus megaterium 410. the isomerization reaction was detected as a shift in the absorbance maximum from 330 nm, the maximum for maleylpyruvate, to 345 nm, the maximum for fumarylpyruvate, when assayed at ph 8.0. ammonium sulfate precipitation and dialysis of b. megaterium cell extr ... | 1985 | 3926749 |
| [characteristics of the development and biosynthetic activity of a mixed culture of microorganisms]. | the growth of microbial populations and their biosynthesis of proteinases with the fibrinolytic activity were studied in an artificial ecosystem composed of a strain producing the enzymes (nocardia minima 1) and a zero strain (arthrobacter citreus vkm 654). the microorganisms in the association were shown to interact in terms of amensalism via metabolites if the enzyme-producing strain dominated. | 1985 | 3903442 |
| localization of the enantiozymes of 6-hydroxy-nicotine oxidase in arthrobacter oxidans by electron immunochemistry. | during the course of growth of arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-d-nicotine oxidase and 6-hydroxy-l-nicotine oxidase occurred in the presence of dl-nicotine. cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. the results obtained demonstrate that both enzymes are localized in the cytoplasm. | 1985 | 4019415 |
| effect of neuraminidase treatment of cells and effect of soluble glycoproteins on type 3 reovirus attachment to murine l cells. | the effect of pretreatment of murine l cells with bacterial neuraminidases on type 3 reovirus attachment was examined. we observed that such treatments resulted in a 60 to 80% decrease of subsequent attachment of 35s-labeled type 3 reovirus in a time- and dose-dependent manner. this result was specific for removal of cell surface sialic acid residues since the specific neuraminidase inhibitor 2-deoxy-2,3-dehydro-n-acetyl neuraminic acid completely prevented the observed effect. although the tota ... | 1985 | 4057353 |
| enzymes of vitamin b6 degradation. purification and properties of two n-acetylamidohydrolases. | alpha-(n-acetylaminomethylene)succinic acid hydrolase (compound a hydrolase, ec 3.5.1-) and alpha-hydroxymethyl-alpha'-(n-acetylaminomethylene)succinic acid hydrolase (compound b hydrolase, ec 3.5.1-) were purified to homogeneity from pseudomonas ma-1 and arthrobacter cr-7, respectively. the two inducible enzymes catalyze reactions 1 and 2, respectively, which release the first generally useful anabolic intermediates during growth of these organisms with (formula; see text) pyridoxine as a sole ... | 1985 | 3972793 |
| survival of brevibacterium linens during nutrient starvation and intracellular changes. | the present work reports the survival capacity of a strain of brevibacterium linens isolated from a french camembert cheese and the ensuing changes in cell composition. exponentially growing cells were harvested, washed and resuspended with shaking in ph 8.0 buffer at 21 degrees c in the absence of a carbon source. the viability of this strain, assessed with slide cultures, is much less than that of coryneform bacteria isolated from soil samples, even though no cell lysis was detected. intracell ... | 1985 | 3994487 |
| intermolecular fructosyl and levanbiosyl transfers by levan fructotransferase of arthrobacter ureafaciens. | a purified levan fructotransferase preparation from the culture of the bacterium arthrobacter ureafaciens, which produces di-d-fructose 2,6':6,2' dianhydride (difructose anhydride iv) from levan by an intramolecular levan fructosyl transfer (ilft) reaction, was found to produce a trioligofructan and a tetraoligofructan from levan in the presence of levanbiose, indicating the intermolecular fructosyl and levanbiosyl transfer (lft and lbt) reactions. the tri- and tetraoligofructans were identified ... | 1985 | 4030745 |
| in situ immunological determination of basic carbohydrate structures of gangliosides on thin-layer plates. | an immunological method for the determination of the basic carbohydrate structure of gangliosides by using a thin-layer chromatographic immunostaining technique was developed. after high-performance thin-layer chromatography of gangliosides, the chromatogram is treated with a 0.4% polyisobutylmethacrylate solution. arthrobacter ureafaciens neuraminidase is then applied to the separated gangliosides in situ on the chromatographic plate. this procedure will remove both external and internal sialic ... | 1985 | 4037308 |
| siderophore production by the pathogenic yeast, candida albicans. | biochemical assays were used to determine that some strains of candida albicans were capable of simultaneous secretion of both the hydroxamate and phenolate-type siderophores when grown in a deferrated medium at 37 degrees c. all isolants of c. albicans released hydroxamate-type siderophores into the culture medium; whereas, approximately 40% of the strains simultaneously secreted phenolate-type siderophores. the presence of phenolate and hydroxamate-type siderophores in the culture medium was f ... | 1985 | 3161506 |
| isolation and characterization of influenza c virus inhibitor in rat serum. | two hemagglutination inhibitors for influenza c virus were isolated from pooled sera of normal rats by sequential chromatography on blue sepharose cl 6b, ultrogel aca 22, and deae-cellulose. the two inhibitors were identified as alpha 1-macroglobulin and murinoglobulin by comparison with the authentic samples. these inhibitors abolished the hemagglutination by influenza c virus strains but did not affect the hemagglutination by influenza a and b virus strains. hemagglutination inhibition activit ... | 1985 | 2416143 |
| production of organic acids by bacteria isolated from soil, rhizosphere and mycorrhizosphere of pine (pinus sylvestris l.). | the bacteria studied released into the medium ten to eleven organic acids. soil organisms excreted mainly pyruvic and alpha-ketoglutaric acids, while strains from the root zone--gluconic acid and unidentified uronic acid (y2). mean indices of total production of the organic acids by bacteria were in the following order: rhizosphere less than soil less than mycorrizosphere. bacteria from the root zone released into the medium very large amounts of pyruvic, gluconic, and uronic (y2) acids--in some ... | 1985 | 2421547 |
| regulation of 3-ketosteroid-1-en-dehydrogenase activity of arthrobacter globiformis cells by a respiratory chain. | it has been shown that 3-ketosteroid-1-en-dehydrogenase localized in a cytoplasmic membrane donates reducing equivalents to a respiratory chain directly which passes them over to oxygen. microbial hydrocortisone oxidation is coupled with energy generation in the form of the h+ transmembrane potential. electron transfer via a respiratory chain is the limiting stage in the process of hydrocortisone 1-en-dehydrogenation. | 1985 | 4046608 |
| redox reactions in hydrocortisone transformation by arthrobacter globiformis cells. | hydrocortisone and prednisolone transformation by arthrobacter globiformis cells in aerobic and anaerobic conditions was studied. 3-ketosteroid-1-en-dehydrogenase activity was shown to be the major factor regulating the direction of transformation. when it is high (aerobic conditions), the end products of hydrocortisone transformation are prednisolone or its 20 beta-hydroxy derivative. the latter is produced via 1-en-dehydrogenation, which is not a limiting stage of the process. low 3-ketosteroi ... | 1985 | 4046604 |
| the cytotoxic and photodynamic inactivation of micro-organisms by rose bengal. | rose bengal was cytotoxic to the following bacteria at the concentrations given in parentheses (highest concentrations of dye in mol/l at which growth occurred on nutrient medium): brochothrix thermosphacta and deinococcus radiodurans (1 x 10(-6) or less); streptococcus, micrococcus, staphylococcus, bacillus, arthrobacter and kurthia spp. (1 x 10(-5)-1 x 10(-4], and pseudomonas spp. and enterobacteriaceae (5 x 10(-3)-1 x 10(-2) or greater). these organisms were killed rapidly when suspended in i ... | 1985 | 3997691 |
| isolation and characterization of influenza c virus inhibitors in rat serum. | two inhibitors against haemagglutination by influenza c virus were isolated from pooled sera of normal rats by sequential chromatography on blue sepharose cl 6b, ultrogel aca 2, and deae-cellulose. the two inhibitors were identified as alpha 1-macroglobulin and murinoglobulin by comparison with the authentic samples. these inhibitors abolished the haemagglutination by influenza c virus strains but did not affect the haemagglutination by influenza a and b virus strains. haemagglutination inhibiti ... | 1985 | 4060850 |
| sulindac oxidation/reduction by microbial cultures; microbial models of mammalian metabolism. | the oxidation and reduction of the sulphoxide moiety of the anti-inflammatory agent sulindac was investigated to explore microbial systems exhibiting parallels of known mammalian metabolism. of 24 cultures initially screened, four catalysed the expected reactions in analytical studies. arthrobacter species (atcc 19140) and sporobolomyces pararoseus (atcc 11386) produced sulindac sulphide, aspergillus alliaceus (nrrl 315) produced sulindac sulphone, and nocardia corallina (atcc 19070) produced bo ... | 1985 | 4072250 |
| mathematical simulation of pseudo-crystallofermentation of hydrocortisone by arthrobacter simplex. | a mathematical model has been developed to simulate the pseudo-crystallofermentation process of hydrocortisone by arthrobacter simplex. the model describing the process included considerations on the kinetics of microbial growth, the rate of enzyme formation, the rate of substrate dissolution, the rate of solute crystallization, and the kinetics of enzymatic reaction. the system of equations was solved numerically by the runge-kutta-gill method. the good agreement between prediction and experime ... | 1985 | 18553666 |
| use of the mushroom tissue block rapid pitting test to detect brown blotch pathogens. | isolates of an arthrobacter species known to produce a positive host pathogenicity test were evaluated by the mushroom tissue block rapid pitting test. all 11 isolates of the arthrobacter species yielded a positive test response. the time required for a positive test was the same for pseudomonas tolaasi and the arthrobacter species. | 1985 | 16346775 |
| selective isolation of bacillus sphaericus from soil by use of acetate as the only major source of carbon. | a simple chemically defined medium containing sodium acetate (37 or 74 mm) as the only major source of carbon was inoculated with soil from various locations. the bacteria which grew most rapidly at 30 degrees c were almost entirely strains of bacillus sphaericus and arthrobacter species. pasteurization of the soils made the medium selective for b. sphaericus. several new strains were isolated, and the peptidoglycans of their cell walls were examined. | 1985 | 16346815 |
| survival of bacteria and fungi in relation to water activity and the solvent properties of water in biopolymer gels. | survival of bacteria (rhizobium, agrobacterium, and arthrobacter spp.), fungal spores (penicillium sp.), and yeasts (saccharomyces sp.) was studied in relation to water activity (a(w)) and the presence of nutritive solutes. the cells were entrapped in polysaccharide gels, as is done to immobilize cells or enzymes, and then dehydrated. the number of living cells (10 g of dry polymer) remained constant for periods of storage of >3 years at 28 degrees c when the inocula were kept at an a(w) of <0.0 ... | 1985 | 16346829 |
| characterization of subsurface bacteria associated with two shallow aquifers in oklahoma. | the bacterial microflora of two shallow aquifers (saturated subsurface zones) in oklahoma was characterized by direct observation with light and electron microscopy, by plating, and by examination of colony morphology and distribution. isolated bacterial strains were also examined. total cell counts varied only slightly (2.9 x 10 to 9.8 x 10 g [dry wt]) from sample to sample, whereas colony counts varied widely (6.3 x 10 to 6.5 x 10 cfu g [dry wt]). colony counts on nutritionally rich media were ... | 1985 | 16346877 |
| production and characterization of a polymer from arthrobacter sp. | an arthrobacter sp. isolated from a glucose-sucrose agar plate was found to produce a neutral, extremely viscous, opalescent extracellular polymer. growth, polymer production, and rheological properties and chemical composition of the isolated polymer were examined. the polymer was found to be substantially different from other arthrobacter polymers. some unusual properties included irreversible loss of viscosity with high temperature and degradation of the polymer during fermentation and upon s ... | 1985 | 16346883 |
| distribution of brown blotch bacteria in wild and cultivated species of basidiomycetes. | wild and cultivated basidiomycetes species were cultured to determine the distribution of bacteria causing brown blotch disease of agaricus bisporus. colonies from each basidiocarp were screened for brown blotch organisms by the white line and host pathogenicity tests. isolates causing brown blotch were identified as pseudomonas tolaasi and an arthrobacter species. | 1984 | 16346653 |
| degradation of 4-chlorobenzoic acid by arthrobacter sp. | a mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. an organism, identified as arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy source in pure culture. this organism (strain tm-1), dehalogenated 4-chlorobenzoate as the initial step in the degradative pathway. the product, 4-hydroxybenzoate, was further metabo ... | 1984 | 16346660 |
| production of d-tagatose from dulcitol by arthrobacter globiformis. | a process for the bacterial oxidation of dulcitol to d-tagatose has been developed. the strain arthrobacter globiformis st48 used in this fermentation was isolated from soil. the yield of d-tagatose accumulated in the medium from dulcitol was as high as 85%. about 14 g of d-tagatose crystals was isolated from 1 liter of 2% dulcitol medium. | 1984 | 16346663 |
| formation and identification of interfacial-active glycolipids from resting microbial cells. | resting cells of arthrobacter sp. strain dsm2567 incubated in the presence of various mono-, di-, or trisaccharides biosynthesized different glycolipids. all eight glycolipids, containing the corresponding carbohydrate moiety and one, two, or three alpha-branched beta-hydroxy fatty acids, were produced when mannose, glucose, cellobiose, maltose, and maltotriose were used as carbon sources in a simple phosphate buffer. the structures of the compounds were elucidated by means of h and c nuclear ma ... | 1984 | 16346628 |
| immunochemical properties of mannan-protein complex isolated from viable cells of saccharomyces cerevisiae 4484-24d-1 mutant strain by the action of zymolyase. | viable cells of saccharomyces cerevisiae 4484-24d-1 mutant strain were treated with an arthrobacter sp. beta-1,3-glucanase, zymolyase-60,000, in the presence of a serine protease inhibitor, phenylmethylsulfonyl fluoride. fractionation of the solubilized materials with cetavlon (cetyltrimethylammonium bromide) yielded a purified mannan-protein complex, which had a molecular weight of ca. 150,000, approximately three times higher than that of the mannan isolated from the same cells by the hot-wate ... | 1984 | 6398395 |
| the inactivation of glucosamine synthetase from bacteria by anticapsin, the c-terminal epoxyamino acid of the antibiotic tetaine. | incubation of anticapsin with the purified glucosamine synthetase (2-amino-2-deoxy-d-glucose-6-phosphate ketol-isomerase, amino transferring, ec 5.3.1.19) from escherichia coli, pseudomonas aeruginosa, arthrobacter aurescens and bacillus thuringiensis led to the formation of an inactive enzyme irreversibly modified. the inactivation reaction followed pseudo-first-order kinetics. the rate of the inactivation reaction at various concentrations of anticapsin exhibited saturation kinetics, implying ... | 1984 | 6426523 |
| specificity and mechanism of ferrioxamine-mediated iron transport in streptomyces pilosus. | although the ferrioxamines are an important and well-characterized class of siderophores produced by several species of nocardia, streptomyces, micromonospora, arthrobacter, chromobacterium, and pseudomonas, no studies of the mechanism of ferrioxamine-mediated iron uptake have been performed for an organism which produces the siderophore. this is the first report of metal transport in streptomyces pilosus mediated by the native ferrioxamines b, d1, d2, and e. 55fe accumulation in these ferrioxam ... | 1984 | 6480557 |
| interaction between rat peritoneal macrophages and sialidase-treated erythrocytes: biochemical and morphological studies. | rat peritoneal macrophages bind and phagocytose homologous, sialidase-treated erythrocytes at a rate depending on the number of red cells and the amount of sialic acids released. vibrio cholerae sialidase only partially (75%) removes the sialic acid residues from rat erythrocytes, whereas with arthrobacter ureafaciens sialidase complete desialylation is possible. analysis of the sialic acids by capillary gas-liquid chromatography combined with mass spectrometry (glc-ms) revealed the occurrence o ... | 1984 | 6487288 |
| the origin of the oxygen incorporated during the dehalogenation/hydroxylation of 4-chlorobenzoate by an arthrobacter sp. | an arthrobacter sp. has been shown to dehalogenate 4-chlorobenzoate yielding 4-hydroxybenzoate. experiments with 18o indicate that, in the presence of cell-free extracts, the hydroxyl group which is substituted onto the aromatic nucleus during dehalogenation is derived from water and not from molecular oxygen. dehalogenation therefore is not catalysed by a mixed-function oxidase; instead a novel aromatic hydroxylase is implicated in the reaction. | 1984 | 6497895 |
| [sialidase from arthrobacter ureafaciens]. | 1984 | 6718750 | |
| isolation and partial characterization of plasmid dna from arthrobacter oxidans. | a method for the extraction of the high molecular weight plasmid ao 1 from the gram-positive soil bacterium arthrobacter oxidans is presented. following digestion of this dna with the restriction endonucleases acci, bam hi, eco ri and hind iii, an average molecular mass of 157.8 kb was estimated. this value is in good agreement with the 160 kb size determined previously by electron microscopy (brandsch et al. 1982). using the same method, no plasmid dna was found in strains of the genus arthroba ... | 1984 | 6742952 |
| retrohydroxamate ferrichrome, a biomimetic analogue of ferrichrome. | a new synthetic analogue of ferrichrome, retrohydroxamate ferrichrome, has been examined for biological activity. although spectroscopic evidence indicates that the analogue is a weaker fe(iii) chelator than ferrichrome, retrohydroxamate ferrichrome is indistinguishable from ferrichrome in its growth factor activity for arthrobacter flavescens, and in its potency in antagonizing the antibiotic activity of albomyhcin against bacillus subtilis. it is as active as ferrichrome as a siderophore for t ... | 1984 | 6424674 |
| factors limiting the microbial conversion of sterols to 17-ketosteroids in the presence of metal chelate inhibitors. | bioconversion of sterols to 17-ketosteroids by an arthrobacter species occurred in the presence of hydrophobic metal-chelating agents but the production of 17-ketosteroids (17-ks) was seriously limited by the rapid loss of the viability of cells in the presence of these inhibitors. besides, the conversion was inhibited by 17-ks at concentrations of 500 ppm or more. the 17-ks formed consisted exclusively of 1,4-androstadiene-3,17-dione (add) and 4-androstene-3,17-dione (ad) and these were found i ... | 1984 | 6745816 |
| effect of ph on production of cytokinin-like substances by bacteria isolated from soil, rhizosphere and mycorrhizosphere of pine (pinus silvestris l.). | cytokinin-like substances were produced in higher amounts by soil isolates than by those isolated from the rhizosphere and mycorrhizosphere. however more bacterial strains isolated from the rhizosphere and mycorrhizosphere were capable of synthesizing cytokinin-like compounds. a distinct effect of ph on the production of these substances was found. it is suggested that zeatin and zeatin riboside are likely to be among the cytokinin-like substances detected. | 1984 | 6205554 |
| [immobilization of cells with steroid-1,2-dehydrogenase activity on different carriers]. | 1984 | 6377834 | |
| [h-nmr spectroscopy. specificity of microbial sialidases against complex substrates]. | the specificities of one viral and five bacterial sialidases were investigated by 1h-nmr-spectroscopy with substrates or substrate mixtures containing two sialic acid residues of different linkage types. this technique allows - in contrast to the methods used before - the simultaneous determination of the rates of hydrolysis of both neuac linkages in a single experiment. the substrate specificities of the enzymes are discussed on the basis of the relation of the rate constants k/k'. the data obt ... | 1984 | 6096253 |
| extracellular siderophores from aspergillus ochraceous. | a large number of iron-chelating compounds (siderophores) were isolated from supernatants of iron-deficient cultures of a mold isolate, subsequently identified as aspergillus ochraceous . siderophores in their iron chelate form were purified to homogeneity by using bio-gel p2, silica gel, and c-18 bonded silica gel (reverse-phase) columns. most of these compounds, as identified by 1h and 13c nuclear magnetic resonance spectroscopy and x-ray crystallography, belong to the ferrichrome family. the ... | 1984 | 6233261 |
| inhibition of glucosamine-6-phosphate synthetase from bacteria by anticapsin. | on the basis of kinetic studies on glucosamine-6-phosphate synthetase (ec 5.3.1.19) from six bacteria sources it has been shown that the epoxyamino acid anticapsin, a glutamine analog, is a competitive inhibitor of the enzyme in regard to glutamine with km value of 10(-4) m and ki varying from 10(-7) to 10(-6) m. unlike other glutamine analogs like 6-diazo-5-oxo-l-norleucine, chloropentanoic acid, l-alpha-amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid or albizziin, anticapsin is not generall ... | 1984 | 6430854 |
| generic composition and physiological and cultural properties of heterotrophic bacteria isolated from soil, rhizosphere and mycorrhizosphere of pine (pinus silvestris l.). | among the bacteria studied arthrobacter globiformis was predominating in the root zone, while in the non-rhizosphere soil most numerous were bacillus circulans and a. globiformis. ammonifiers were more numerous among the root zone bacteria than among the root free soil organisms. the reverse was noted with bacteria capable of hydrolysing starch, cellulose, pectin and chitin. | 1984 | 6209931 |
| interaction between bacterial metabolites and some pesticides. ii. change of phytotoxicity of the herbicide roneet by the phenolic metabolites of arthrobacter sp. | the bacteria from arthrobacter genus isolated from sugar beet rhizosphere were found to produce gallic, protocatechuic, p-hydroxybenzoic, syryngic, vanillic, veratric acids, p-quinone and two unidentified phenolic compounds. the mixture of the bacterial phenolic compounds increased the phytotoxicity of roneet, inhibiting the germination of wheat. model experiments showed that the phenolic acids used with the herbicide roneet increased its phytotoxicity and p-hydroxybenzoic acid was found to be t ... | 1984 | 6209927 |
| fish egg polysialoglycoproteins: circular dichroism and proton nuclear magnetic resonance studies of novel oligosaccharide units containing one sialidase-resistant n-glycolylneuraminic acid residue in each molecule. | long-core units having the common sequence ga1nac beta 1 leads to 4(neugc2 leads to 3)ga1nac beta 1 leads to 3gal beta 1 leads to 4ga1 beta 1 leads to 3ga1nac are one of the major constituents of rainbow trout egg polysialoglycoproteins. the existing ambiguity regarding the anomeric configuration of the sialidase-resistant unsubstituted sialyl group present in this novel type of oligosaccharide chains has been resolved by a circular dichroism difference spectral method. the fact that the negativ ... | 1984 | 6320865 |
| purification and properties of n-acetylneuraminate lyase from escherichia coli. | n-acetylneuraminate lyase [n-acetylneuraminic acid aldolase ec 4.1.3.3] from escherichia coli was purified by protamine sulfate treatment, fractionation with ammonium sulfate, column chromatography on deae-sephacel, gel filtration on ultrogel aca 44, and preparative polyacrylamide gel electrophoresis. the purified enzyme preparation was homogeneous on analytical polyacrylamide gel electrophoresis, and was free from contaminating enzymes including nadh oxidase and nadh dehydrogenase. the enzyme c ... | 1984 | 6389524 |
| [growth kinetics of microorganisms with various ecological strategies in a dialysis culture at low specific growth rates]. | a dialysis culture was found to be most suitable for studying the metabolism of microorganisms at a low specific growth rate. the biomass of all microorganisms studied in the dialysis culture increased with time in a linear fashion; hence, the energy spent for growth decreased in proportion to a decrease in the specific growth rate. microorganisms growing in oligotrophous environment (arthrobacter globiformis and lipomyces tetrasporus ) spent much less energy comparing to microorganisms from eut ... | 1984 | 6429490 |
| novel oligosaccharide chains on polysialoglycoproteins isolated from rainbow trout eggs. a unique carbohydrate sequence with a sialidase-resistant sialyl group, dga1nac beta 1 leads to 4(neugc2 leads to 3)dga1nac. | a series of novel carbohydrate chains all possessing a hitherto unknown trisaccharide unit, ga1nac beta 1 leads to 4-(neugc2 leads to 3)ga1nac beta 1 leads to, have been isolated from trout egg polysialoglycoproteins, a new class of glycoproteins, on alkali-borohydride treatment. on the basis of chemical (methylation, smith degradation, and hydrazinolysis-nitrous deamination) and direct-probe mass spectrometric methods. deamination) and direct-probe mass spectrometric methods, the structures of ... | 1984 | 6320864 |
| examination of protein sequence homologies: i. eleven escherichia coli l7/l12-type ribosomal "a" protein sequences from eubacteria and chloroplast. | seven complete and four partial sequences of escherichia coli l7/l12-type ribosomal "a" proteins obtained from various bacteria (e. coli, bacillus subtilis, micrococcus lysodeikticus, rhodopseudomonas spheroides, desulfovibrio vulgaris, streptomyces griseus, bacillus stearothermophilus, clostridium pasteurianum, arthrobacter glacialis, and vibrio costicola) and spinach chloroplast have been reexamined using a computer program that searches for homologous tertiary structures. comparison matrices ... | 1984 | 6443314 |
| [microbiological studies of ground water polluted with hydrocarbons. 1. quantitative and qualitative distribution of bacterial populations]. | the groundwater, polluted with hydrocarbons, showed large cell numbers during one year of examination: 10(3)-10(4) cfu/ml grown on n-agar after 5 days at 20 degrees c, 10(4)-10(5) cfu/ml grown on water-agar after 8 weeks at 20 degrees c and total cell numbers of 10(5)-10(7)/ml. the bacteria, which were isolated from n-agar, were chiefly pseudomonas, flavobacterium, acinetobacter, and other gramnegative species, arthrobacter and bacillus as members of grampositive bacteria. furthermore the follow ... | 1984 | 6524146 |
| the interaction of substrate-related ketals with bacterial and viral neuraminidases. | arthrobacter sialophilus neuraminidase catalyzes the hydration of 5-acetamido-2,6-anhydro-3,5-dideoxy-d-glycero-d-galacto-non-2-enonic acid (2,3-dehydro-acneu) with km and kcat values of 8.9 x 10(-4) m and 6.40 x 10(-4) s-1, respectively. the methyl ester of 2,3-dehydro-acneu as well as 2,3-dehydro-4-epi-acneu are also hydrated by the enzyme. the product resulting from the enzymatic hydration of 2,3-dehydro-acneu is n-acetylneuraminic acid. a series of derivatives of 2,3-dehydro-acneu (k1, 1.60 ... | 1983 | 6830254 |
| [detection of teichoic acids in cells of bacteria of the genus arthrobacter]. | 1983 | 6628186 | |
| [the phosphoketolase system of carbohydrate cleavage in saprophytic mycobacteria]. | 1983 | 6435980 | |
| action of levan fructotransferase of arthrobacter ureafaciens on levanoligosaccharides. | levan fructotransferase of the bacterium arthrobacter ureafaciens, which produces di-d-fructose 2,6':6,2' dianhydride (difructose anhydride iv) from levan by an intramolecular transfructosylation reaction, was purified to give a single protein band of pi 4.5-4.7 on isoelectric focusing. it had a molecular weight of 128,000 on gel-filtration on sephadex g-200 and 60,000 on sds-polyacrylamide disc gel-electrophoresis, suggesting that the enzyme is composed of two identical subunits. the shortest l ... | 1983 | 6654872 |
| immobilization of proteins on partially hydrolyzed agarose beads. | treatment of agarose beads with mild acid (0.2 m hcl, 55 degrees c, several hours) hydrolyzes some of the glycosidic bonds between d-galactosyl residues and 3,6-anhydro-l-galactosyl residues, and thus produces aldehydo-groups useful for immobilization of amino compounds by reductive amination with nacnbh3. more than 20 mg (0.3 mumol) of bovine serum albumin could be coupled per gram of partially hydrolyzed agarose beads. arthrobacter neuraminidase immobilized by this method was useful for desial ... | 1983 | 6660514 |
| [coupling between 3-ketosteroid-delta'-dehydrogenase and the respiratory chain in the bacterium arthrobacter globiformis]. | intact bacterial cells and their cytoplasmic membranes obtained by ultrasonic desintegration are able to induce dehydration of hydrocortisone to prednisolone that is coupled with o2 uptake. this reaction is inhibited by cyanide and 2-n-nonyl-4-hydroxyquinoline-n-oxide (hoqno). oxidation of hydrocortisone results in cytochrome reduction both in intact cells and in cytoplasmic membranes. aerobic dehydration of hydrocortisone is linked with transmembrane potential generation. the data obtained sugg ... | 1983 | 6639991 |
| use of specialized microbial strains in the treatment of industrial waste and in soil decontamination. | 1983 | 6357840 | |
| purification of the flavoproteins 6-hydroxy-d- and 6-hydroxy-l-nicotine oxidase using hydrophobic affinity chromatography. | a systematic study of the homologous series of omega-aminoalkyl-agaroses revealed differences in the affinities of 6-hydroxy-d- and 6-hydroxy-l-nicotine oxidase. in contrast to supports with nonpolar alkyl chains, omega-aminoalkyl-agarose showed high affinity towards the l-specific enzyme, while the d-specific oxidase was bound most firmly by omega-aminododecyl-agarose. 6-hydroxy-l-nicotine oxidase could be desorbed by 1.3m nacl only in the presence of the substrate l-6-hydroxynicotine. using th ... | 1983 | 6618442 |
| detoxification of pesticides by microbial enzymes. | 1983 | 6357841 | |
| enzymatic synthesis of flavin adenine dinucleotide. | a new and simple enzymatic method for the synthesis of flavin adenine dinucleotide (fad) from flavin mononucleotide by the transadenylylation reaction using microbial cells is described. among various microorganisms tested, artherobacter globiformis ifo 12138 and two soil bacteria were selected as useful enzyme sources. under suitable reaction conditions, the amount of fad synthesized was 2.25 mumol/ml with cells of a. globiformis. the transadenylylation reaction could be coupled with the atp su ... | 1983 | 6089661 |
| siderophore-mediated iron uptake in different strains of anabaena sp. | anabaena sp. strain 6411, which produces the dihydroxamate siderophore schizokinen to facilitate iron uptake, is also capable of using the related siderophore aerobactin. the two siderophores compete for the same iron transport system, but there is a markedly higher affinity for ferric schizokinen than for ferric aerobactin. the trihydroxamate siderophore ferrioxamine b is far less effective as an iron donor in this organism. anabaena sp. strain 7120 appears to be closely related to strain 6411. ... | 1983 | 6227608 |
| the specificity of viral and bacterial sialidases for alpha(2-3)- and alpha(2-6)-linked sialic acids in glycoproteins. | the anomeric specificity of six sialidases (vibrio cholerae, arthrobacter ureafaciens, clostridium perfringens, newcastle disease virus, fowl plague virus and influenza a2 virus sialidases) was assessed with sialylated antifreeze glycoprotein, ovine submandibular gland glycoprotein and alpha 1-acid glycoprotein, resialylated specifically in alpha(2-3) or alpha(2-6) linkage with n-acetylneuraminic acid or n-glycolylneuraminic acid using highly purified sialyltransferases. the rate of release of s ... | 1983 | 6301560 |
| a phylogenetic analysis of the family dermatophilaceae. | the comparative analysis of the 16s ribosomal ribonucleic acid (rrna) of geodermatophilus obscurus dsm 43060 and dermatophilus congolensis dsm 43037 revealed that these members of the family dermatophilaceae were only remotely related. while g. obscurus represented an individual and separate line of descent within the phylogenetically defined order actinomycetales, d. congolensis was closely related to representatives of arthrobacter, micrococcus, cellulomonas, brevibacterium, promicromonospora ... | 1983 | 6195302 |