Publications

mechanism for aldose-ketose interconversion by d-xylose isomerase involving ring opening followed by a 1,2-hydride shift.	the active site and mechanism of d-xylose isomerase have been probed by determination of the crystal structures of the enzyme bound to various substrates, inhibitors and cations. ring-opening is an obligatory first step of the reaction and is believed to be the rate-determining step for the aldose to ketose conversion. the structure of a complex with a cyclic thio-glucose has been determined and it is concluded that this is an analogue of the michaelis complex. at -10 degrees c substrates in cry ...	1990	2319597
a repeated decapeptide motif in the c-terminal domain of the ribosomal rna methyltransferase from the erythromycin producer saccharopolyspora erythraea.	re-analysis of the primary structure of the ribosomal rna n-methyltransferase that confers self-resistance on the erythromycin-producing bacterium saccharopolyspora erythraea has confirmed the presence of a c-terminal domain containing extensive repeat sequences. nine tandem repeats can be discerned, with a decapeptide consensus sequence ggrx(h/r)gdrrt, although no single residue is wholly invariant. this highly polar, potentially flexible domain, which is predicted to adopt either a random coil ...	1990	2335200
methylamine oxidase from arthrobacter p1 as a prototype of eukaryotic plasma amine oxidase and diamine oxidase.	methylamine oxidase (maox) from gram-positive soil bacterium arthrobacter p1 catalyzes the oxidation of ch3nh2 to h2c = o and nh4+ via reduction of o2 to h2o2. past work indicates that maox is similar to mammalian plasma amine oxidase (pao) and diamine oxidase (dao), plant dao, and yeast peroxisomal amine oxidase (yao). all have mr congruent to 170,000 and are composed of 2 identical subunits, each of which contains 1 atom of cu(ii) and one molecule of quinonoid cofactor. herein, we report furth ...	1990	1965196
effect of organic amendments on bacterial multiplication in lake water.	in cayuga lake water amended with 30 micrograms of glucose or amino acids per ml, an added strain of pseudomonas fluorescens and indigenous bacteria grew extensively, pseudomonas sp. b4 and two rhizobia multiplied at a moderate extent, and introduced escherichia coli and klebsiella pneumoniae multiplied but to only a slight degree. the amendments did not enhance growth of micrococcus flavus and arthrobacter citreus, and an asporogenous strain of bacillus subtilis decreased in numbers. the pseudo ...	1990	2372214
functional analysis of the 5' regulatory region and the uug translation initiation codon of the arthrobacter oxidans 6-hydroxy-d-nicotine oxidase gene.	a functional analysis of the arthrobacter oxidans 6-hydroxy-d-nicotine oxidase (6-hdno) gene promoter (-35 region ttgaca and -10 region tatcaat) and the uug translation start codon was performed using site-directed mutagenesis. deletion of the c residue from the -10 promoter region or mutations introduced upstream of the -10 region resulted in an increased 6-hdno expression in escherichia coli cells in vivo and in both e. coli and a. oxidans coupled transcription-translation systems in vitro. fr ...	1990	2381422
characterization of phi ga1, an inducible phage particle from brevibacterium flavum.	eighteen related strains of arthrobacter, brevibacterium and corynebacterium were used as indicator strains in an attempt to isolate corynephages from a large number of soils and from waste-water samples. although no phages capable of producing plaques were isolated, one of the indicator strains used, brevibacterium flavum atcc 14067, was lysogenic for the inducible phage phi ga1. this phage was not observed to form plaques on any of the strains tested. phi ga1 is of b1-morphotype with a linear ...	1990	2391491
different types of dienelactone hydrolase in 4-fluorobenzoate-utilizing bacteria.	of various benzoate-utilizing bacteria tested, alcaligenes eutrophus 335, a. eutrophus h16, a. eutrophus jmp222, a. eutrophus jmp134, alcaligenes strain a7, and pseudomonas cepacia were able to grow with 4-fluorobenzoate as the sole source of carbon and energy. p. cepacia also utilizes 3-fluorobenzoate. except for a. eutrophus jmp134, which is known to grow with 2,4-dichlorophenoxyacetate and 3-chlorobenzoate (r. h. don and j. m. pemberton, j. bacteriol. 145:681-686, 1981), the strains were unab ...	1990	2394679
reductive trapping of substrate to methylamine oxidase from arthrobacter p1.	methylamine oxidase (ec 1.4.3.6) from arthrobacter p1 was inactivated by nacnbh3 in the presence of [14c]benzylamine, leading to the incorporation of 1 mol of radiolabeled substrate/mol of enzyme subunit at complete inactivation. by contrast, no labeling of enzyme was observed using [3h]nacnbh3 as reductant. these results are analogous to those previously reported for the eukaryotic enzyme, bovine serum plasma amine oxidase [(1987) j. biol. chem. 262, 962-965]. the observed pattern of labeling i ...	1990	2155832
a survey of the microflora of raw and pasteurized milk and the sources of contamination in a milk processing plant in addis ababa, ethiopia.	the microorganisms present in raw and pasteurized milk and the sources of contamination in the milk after it had arrived at the processing plant in addis ababa were studied. the lowest count registered for raw milk samples was 4 x 10(7) cfu/ml while the highest was 1 x 10(9) cfu/ml. pasteurized milk had mesophilic aerobic counts of 7 x 10(5) cfu/ml as it left the pasteurizing unit, but the population increased 2- to 4-fold as a result of subsequent contamination. of the total counts in raw milk, ...	1990	2345191
rapid detection of extended ganglio-series gangliosides with terminal galnac beta 1-4gal sequence on high performance thin layer chromatography plates.	the mouse monoclonal antibody 2d4, which recognizes the terminal galnac beta 1-4gal-disaccharide of ggose3cer and ggose5cer, was used for the detection of ganglio-series gangliosides. the method involves separation of gangliosides on thin layer chromatography plates, followed by silica gel fixation, arthrobacter ureafaciens neuraminidase treatment and final immunostaining of desialylated gangliosides with the monoclonal antibody 2d4. both neuraminidase and the hybridoma 2d4 producing the specifi ...	1990	2350601
sites of cadmium uptake in bacteria used for biosorption.	electron microscopy and x-ray spectroscopy were used to determine location and type of cadmium biosorption on and in bacteria, some of which produced extracellular polymers. examined arthrobacter and pseudomonas species appear to have detoxification systems that precipitate cadmium internally irrespective of whether or not they excrete polymers. capsular klebsiella aerogenes strains showed minimal intracellular uptake but over a 5-100 mg dm-3 cd range produced the highest net metal removal level ...	1990	1366533
action of levan fructotransferase of arthrobacter ureafaciens on three oligosaccharides containing a bifurcose residue.		1990	1368538
purification and some properties of beta-fructofuranosidase i from arthrobacter sp. k-1.	arthrobacter sp. k-1, isolated from soil, produces beta-fructofuranosidase. this enzyme preparation was separated into three fractions (i, ii, and iii) by butyl-toyopearl 650 m column chromatography. the beta-fructofuranosidase i was purified to homogeneity by disc-electrophoresis after consecutive column chromatographies. the enzyme had a molecular weight of 52,000 by sds-polyacrylamide gel electrophoresis and 51,000 by gel filtration with ultrogel aca 44, and an isoelectric point of 4.3. the e ...	1990	1368550
immobilization of arthrobacter simplex in a thermally reversible hydrogel: effect of temperature cycling on steroid conversion.	arthrobacter simplex cells, which convert the steroid hydrocortisone to prednisolone, have been entrapped in a thermally reversible hydrogel. such hydrogels exhibit a lower critical solution temperature (lcst) wherein the gel shrinks and deswells when it is warmed through its lcst, and then reversibly expands and reswells when it is cooled below the lcst. the immobilized cell-hydrogel system has been thermally cycled between two temperatures, each below the lcst. the upper temperature was select ...	1990	18592505
adhesion of an amylolytic arthrobacter sp. to starch-containing plastic films.	cells of the amylolytic bacterium kb-1 (thought to be an arthrobacter sp.) adhered ( approximately 70%) to the surface of plastic films composed of starch-poly (methylacrylate) graft copolymer (starch-pma), but did not adhere (<10%) to films composed of polymethylacrylate (pma), polyethylene (pe), carboxymethyl cellulose, or a mixture of pe plus poly (ethylene-coacrylic acid) (eaa), starch plus pe, or starch plus pe and eaa. about 30% of the cells adhered to gelatinized insoluble starch. dithiot ...	1990	16348173
microbial metabolism of tholin.	in this paper, we show that a wide variety of common soil bacteria are able to obtain their carbon and energy needs from tholin (a class of complex organic heteropolymers thought to be widely distributed through the solar system; in this case tholin was produced by passage of electrical discharge through a mixture of methane, ammonia, and water vapor). we have isolated aerobic, anaerobic, and facultatively anaerobic bacteria which are able to use tholin as a sole carbon source. organisms which ...	1990	11538367
effect of ph and inoculum size on phenol degradation by bacteria isolated from landfill waste.	four aerobic species of mesophilic bacteria which catabolise phenol as the sole carbon source were isolated from landfill waste, and identified as acinetobacter, arthrobacter, micrococcus and nocardia spp. in vitro studies in defined medium revealed variations in abilities of the isolates to catabolise phenol, and in ph growth optima ranging from 6.8 to 7.6. the ph of culture media decreased with growth, and phenol catabolism was impeded below ph 5.4. phenol catabolism in landfill leachate occur ...	1989	15092404
isolation and preliminary characterization of hydroxamic acids formed by nitrogen-fixing azotobacter chroococcum b-8.	the free-living diazotroph azotobacter chroococcum b-8 responded to iron-limited growth conditions by forming hydroxamic acids and an 85,000-dalton outer membrane protein. the fe(iii)-binding hydroxamate compounds stimulated the growth of arthrobacter flavescens jg-9 and gave a positive csaky reaction for bound hydroxylamines. the hydroxamates were isolated from liquid cultures by benzyl alcohol extraction and purified by size exclusion chromatography and high-performance liquid chromatography. ...	1989	16347843
degradation of 4-chlorobenzoate by facultatively alkalophilic arthrobacter sp. strain sb8.	a facultative alkalophile capable of utilizing 4-chlorobenzoate (4-cba), strain sb8, was isolated from soil with an alkaline medium (ph 10.0) containing the haloaromatic compound as the carbon source. the strain, identified as an arthrobacter sp., showed rather extensive 4-cba-degrading ability. 4-cba utilization by the strain was possible in the alkaline medium containing up to 10 g of the compound per liter. the 4-cba-dechlorinating activity of resting cells was almost completely uninhibited b ...	1989	16347854
protozoan response to the addition of bacterial predators and other bacteria to soil.	representatives of several categories of bacteria were added to soil to determine which of them might elicit responses from the soil protozoa. the various categories were nonobligate bacterial predators of bacteria, prey bacteria for these predators, indigenous bacteria that are normally present in high numbers in soil, and non-native bacteria that often find their way in large numbers into soil. the soil was incubated and the responses of the indigenous protozoa were determined by most-probable ...	1989	16347983
induction and purification of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae grown in ovalbumin.	arthrobacter protophormiae produced a high level of extracellular endo-beta-n-acetylglucosaminidase when cells were grown in a medium containing ovalbumin. the enzyme was induced by the glycopeptide fraction of ovalbumin prepared by pronase digestion. production of the enzyme was also induced by glycoproteins such as yeast invertase and bovine ribonuclease b but not by monosaccharides such as mannose, n-acetylglucosamine, and galactose. the enzyme was purified to homogeneity as demonstrated by p ...	1989	16348072
kinetic model of hydrocortisone 1-en-dehydrogenation by arthrobacter globiformis.	a kinetic model of the hydrocortisone-to-prednisolone transformation by arthrobacter globiformis is constructed using the experimental data obtained in studies of this process. besides adequately describing experimental data, the model allows one to determine the relation between hydrocortisone oxidation and the level of endogenous substrates in bacterial cells, and the relation between the saturating concentration of hydrocortisone in the enzymic system of bacteria and the content of endogenous ...	1989	18587966
kinetic model for the regulation of redox reaction in steroid transformation by arthrobacter globiformis cells.	a kinetic model of hydrocortisone transformation was developed in studies of the kinetics of biochemical systems. the regulatory bases of the model are the biosynthesis of steroid-transforming enzymes and their activity, the level of endogenous substrates, the respiratory chain activity, and the initial concentrations of reagents. when compared, the experimental data completely coincide with the results of the computer modeling, the coincidence being not only qualitative but also quantitative. i ...	1989	18587967
binding of fad to 6-hydroxy-d-nicotine oxidase apoenzyme prevents degradation of the holoenzyme.	expression of the 6-hydroxy-d-nicotine oxidase (6-hdno) gene from arthrobacter oxidans cloned into escherichia coli showed a marked temperature-dependence. transformed e. coli cells grown at 30 degrees c exhibited a several-fold higher 6-hdno activity than did cells grown at 37 degrees c. this effect did not depend on the promoter used for expression of the cloned gene in e. coli, nor was it an effect of 6-hdno mrna instability at 37 degrees c. studies performed in vivo and in vitro revealed tha ...	1989	2649085
crystallisation and preliminary analysis of glucose isomerase from streptomyces albus.	the glucose isomerase of streptomyces albus has been crystallised from a dilute solution of magnesium chloride buffered at a ph of 6.8-7.0. the crystals are in the space group i222 with cell dimensions a = 93.9 a, b = 99.5 a and c = 102.9 a. there is one monomer of the tetrameric molecule per asymmetric unit of the crystal and the packing density is 2.93 a3.da-1. the tetramer sits on the 222 symmetry point of the crystal. native data have been recorded to a resolution of 1.9 a and the crystals d ...	1989	2651156
covalent cofactor binding to flavoenzymes requires specific effectors.	modification by covalent fad attachment to a histidine residue via an 8 alpha-(n3-histidyl)-riboflavin linkage occurs in several flavoenzymes. among them is 6-hydroxy-d-nicotine oxidase (6-hdno) of arthrobacter oxidans and the flavoprotein subunits of the fumarate reductase and succinate dehydrogenase complex of escherichia coli and other bacterial and eukaryotic cells. we found that 6-hdno holoenzyme formation from apo-6-hdno, monitored by [14c]fad incorporation and increase in enzyme activity, ...	1989	2659351
structural analysis of phospho-d-mannan-protein complexes isolated from yeast and mold form cells of candida albicans nih a-207 serotype a strain.	the immunochemical properties between phospho-d-mannan-protein complexes of yeast (y) and mycelial (m) forms of candida albicans nih a-207 (serotype a) strain were compared. hydrolysis of the y-form complex gave a mixture of beta-(1----2)-linked d-mannooligosaccharides consisting mainly of tri- and tetra-ose, whereas the m-form complex gave preponderantly d-mannose. the antiserum against y-form cells exhibited a lower reactivity with the m-form than with the y-form complex, whereas the antiserum ...	1989	2663154
structural study of phosphomannan of yeast-form cells of candida albicans j-1012 strain with special reference to application of mild acetolysis.	structural analysis of the phosphomannan isolated from yeast-form cells of a pathogenic yeast, candida albicans j-1012 strain, was conducted. treatment of this phosphomannan (fr. j) with 10 mm hcl at 100 degrees c for 60 min gave a mixture of beta-1,2-linked manno-oligosaccharides, from tetraose to biose plus mannose, and an acid-stable mannan moiety (fr. j-a), which was then acetolyzed by means of an acetolysis medium, 100:100:1 (v/v) mixture of (ch3co)2o, ch3cooh, and h2so4, at 40 degrees c fo ...	1989	2665649
assay for sialidase using erythrocytes and peroxidase-labeled peanut lectin.	a rapid and sensitive sialidase assay method based on peroxidase-labeled peanut lectin (pna) binding to desialylated erythrocyte is described. formalinized sheep erythrocytes were used both as a stable substrate for sialidase and as a target for the lectin. in the case of sialidases from vibrio cholerae and arthrobacter ureafaciens, a linear relationship was observed between the amount of peroxidase-labeled pna bound to erythrocytes and the enzyme amount. binding of the lectin to sialidase-treat ...	1989	2688936
two structural types of mercury reductases and possible ways of their evolution.	structural differences have been revealed among mercury reductases of immunologically unrelated types from gram-positive bacteria: enzymes of one immunological type have a molecular mass of 62-69 kda and seem to contain an n-terminal extension of 5-15 kda, which is easily cleaved by trypsin and chymotrypsin; enzymes of the other immunological type have a molecular mass of 52-57 kda and are resistant to proteolysis. the existence of at least two different lines in the evolution of mercury reducta ...	1989	2714439
inhibition of mouse liver sialidase by the root of scutellaria baicalensis.	of 266 chinese crude drugs, the hot water extract from the root of scutellaria baicalensis showed potent mouse liver sialidase inhibitory activity; in addition, wogonin, wogonin glucuronide, baicalein, and baicalin were identified as the inhibitors. these flavonoids showed almost the same inhibitory activity when 50-125 micrograms/ml doses of the samples were used for the assay, while wogonin and baicalein showed a more potent activity than wogonin glucuronide and baicalin at the lower concentra ...	1989	2717686
biodegradation of crude oils.	petroleum from well sites in the gifhorn trough (lower saxony, nw-germany) and the maracaibo basin (venezuela) contained various types of microorganisms capable of degrading crude oils. genetically related oils were inoculated with the isolated microorganisms and the degradation of the oils was followed by chromatographic techniques. parameters important for the reactions (ph, supply of oxygen, nitrogen and phosphorus, reaction medium) were monitored and optimized. the degradation of n-alkanes w ...	1989	2727641
a new nad+-dependent opine dehydrogenase from arthrobacter sp. strain 1c.	a new nad+-dependent opine dehydrogenase was purified to homogeneity from arthrobacter sp. strain 1c isolated from soil by an enrichment culture technique. the enzyme has a molecular weight of about 70,000 and consists of two identical subunits with molecular weights of about 36,000. the enzyme catalyzed a reversible oxidation-reduction reaction of opine-type secondary amine dicarboxylic acids. in the oxidative deamination reaction, the enzyme was active toward unusual opines, such as n-[1-r-(ca ...	1989	2753861
structures of d-xylose isomerase from arthrobacter strain b3728 containing the inhibitors xylitol and d-sorbitol at 2.5 a and 2.3 a resolution, respectively.	the structures of d-xylose isomerase from arthrobacter strain b3728 containing the polyol inhibitors xylitol and d-sorbitol have been solved at 2.5 a and 2.3 a, respectively. the structures have been refined using restrained least-squares refinement methods. the final crystallographic r-factors for the d-sorbitol (xylitol) bound molecules, for 43,615 (32,989) reflections are 15.6 (14.7). the molecule is a tetramer and the asymmetric unit of the crystal contains a dimer, the final model of which, ...	1989	2769749
sialidase from different sources compared for electrophoretically separating serum alkaline phosphatase fractions from liver and bone.	we compared sialidase (neuraminidase; ec 3.2.1.18) from vibrio cholerae, clostridium perfringens, and arthrobacter ureafaciens, seeking to improve the electrophoretic separation of the liver and bone isoenzymes of alkaline phosphatase (ec 3.1.3.1) on cellulose acetate membranes. resolution is decisively determined by the type and activity of sialidase used in the preincubation of serum sample. sialidase from arthrobacter ureafaciens is not suited for this method. for optimal separation of the tw ...	1989	2776324
enzymatic dehalogenation of pentachlorophenol by extracts from arthrobacter sp. strain atcc 33790.	arthrobacter sp. strain atcc 33790 was grown with pentachlorophenol (pcp) as the sole source of carbon and energy. crude extracts, which were prepared by disruption of the bacteria with a french pressure cell, showed no dehalogenating activity with pcp as the substrate. after sucrose density ultracentrifugation of the crude extract at 145,000 x g, various layers were found in the gradient. one yellow layer showed enzymatic conversion of pcp. one chloride ion was released per molecule of pcp. the ...	1989	2793827
[microbiological dehydrogenation of intermediate of fluocinanide acetate].	both compound i and ii are intermediates in fluocinanide acetate synthesis. i could be dehydrogenated to ii in 62% approximately 63% yields by arthrobactor simplex no. a-1, which was selected in our laboratory. when concentration of i was 0.1%, it was transformed so fast that ii could not be accumulated. when concentration was increased to 0.2%, four intermediates iv, v, vi and vii were formed in addition to a little amount of product ii. when concentration of substrate i was increased to 0.5% a ...	1989	2801119
homology and distribution of co dehydrogenase structural genes in carboxydotrophic bacteria.	the 17 (s), 30 (m) and 87 kda (l) subunits of co dehydrogenases from the co-oxidizing bacteria pseudomonas carboxydoflava, pseudomonas carboxydohydrogena and pseudomonas carboxydovorans om5 were isolated and purified. the n-terminal sequences of same subunits from different bacteria showed distinct homologies. dot blot hybridization employing oligonucleotide probes derived from the sequences of the s-subunit of p. carboxydovorans om5 and the m-subunit of p. carboxydohydrogena and dna of the plas ...	1989	2818128
expression of the arthrobacter viscosus penicillin g acylase gene in escherichia coli and bacillus subtilis.	the penicillin g acylase gene cloned from arthrobacter viscosus 8895gu was subcloned into vectors, and the recombinant plasmids were transferred into escherichia coli or bacillus subtilis. both e. coli and b. subtilis transformants expressed the a. viscosus penicillin g acylase. the enzyme activity was found in the intracellular portion of the e. coli transformants or in the cultured medium of the b. subtilis transformants. penicillin g acylase production in the b. subtilis transformants was 7.2 ...	1989	2504107
molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: some soil bacteria possess a highly conserved bph operon.	all the genes we examined that encoded biphenyl/polychlorinated biphenyl (pcb) degradation were chromosomal, unlike many other degradation-encoding genes, which are plasmid borne. the molecular relationship of genes coding for biphenyl/pcb catabolism in various biphenyl/pcb-degrading pseudomonas, achromobacter, alcaligenes, moraxella, and arthrobacter strains was investigated. among 15 strains tested, 5 pseudomonas strains and one alcaligenes strain possessed the bphabc gene cluster on the xhoi ...	1989	2507526
[hydrophilic-hydrophobic properties of microorganisms under various culturing conditions].	the index of hydrophobicity and the hydrophilic-hydrophobic properties of seven microbial cultures were determined by studying their adhesion to n-hexadecane. the index of hydrophobicity was shown to be a stable characteristic of a culture grown in media with different carbon sources. as was found for escherichia coli k-12 and acinetobacter calcoaceticus k-9 whose hydrophobicity indicates were quite different, the character of cell hydration was virtually independent of the growth phase and did ...	1989	2511395
comparison of human, simian, and bovine rotaviruses for requirement of sialic acid in hemagglutination and cell adsorption.	human rotaviruses (wa, kun, mo) showed hemagglutination (ha) only with fixed 1-day-old chicken erythrocytes, and their ha activities were completely destroyed by trypsin activation of virions. simian sa-11 and bovine ncdv had ha activities not only against fixed erythrocytes but also against fresh erythrocytes from various species. their ha activities against fixed erythrocytes were also inhibited by trypsin activation, but those against fresh erythrocytes were not. neuraminidase treatment of fi ...	1989	2549710
nitrogen metabolism in the facultative methylotroph arthrobacter p1 grown with various amines or ammonia as nitrogen sources.	the metabolism of trimethylamine (tma) and dimethylamine (dma) in arthrobacter p1 involved the enzymes tma monooxygenase and trimethylamine-n-oxide (tma-no) demethylase, and dma monooxygenase, respectively. the methylamine and formaldehyde produced were further metabolized via a primary amine oxidase and the ribulose monophosphate (rump) cycle. the amine oxidase showed activity with various aliphatic primary amines and benzylamine. the organism was able to use methylamine, ethylamine and propyla ...	1989	2589850
purification and properties of neuraminidase isozymes in arthrobacter ureafaciens mutant.	an arthrobacter ureafaciens mutant (m1057) capable of producing neuraminidase constitutively was isolated by ntg mutagenesis from a. ureafaciens kms 3663. four molecular species (l, m1, m2, and s) of neuraminidase isozymes were homogeneously purified from the mutant and parent strains by means of deae-cellulose, affinity chromatography, ammonium sulfate precipitation, chromatofocusing, and ultrogel aca44 gel filtration. the molecular weights of l, m1, m2, and s isozymes were shown to be approxim ...	1989	2628425
antitumor effects of an antiangiogenic polysaccharide from an arthrobacter species with or without a steroid.	a sulfated polysaccharide-peptidoglycan complex, ds-4152, isolated from the culture supernatant of an arthrobacter species inhibited angiogenesis and tumor growth and enhanced the antiangiogenic activity of 11 steroid hormones by 2 to 100 times. in the presence of cortisone acetate or tetrahydro s, ds-4152 suppressed chick chorioallantoic membrane angiogenesis and murine tumor m5076 cell-induced s.c. angiogenesis. the antitumor effects of ds-4152 administered in combination with a steroid whose ...	1989	2479471
13c, 15n, and 31p nmr studies on 6-hydroxy-l-nicotine oxidase from arthrobacter oxidans.	the interaction between the apoprotein of 6-hydroxy-l-nicotine oxidase from arthrobacter oxidans and the prosthetic group fad has been investigated by 13c, 15n, and 31p nmr techniques. the fad prosthetic group was selectively enriched in 13c and 15n isotopes by adding isotopically labeled riboflavin derivatives to the growth medium of riboflavin-requiring mutant cells. in the oxidized state the chemical shift of the c(7) and c(8) atoms indicates that the xylene moiety of the isoalloxazine ring i ...	1989	2540800
interaction of n-acetyl-4-epi-d-neuraminic acid with key enzymes of sialic acid metabolism.	in spite of the axially orientated hydroxy group at c-4, the benzyl alpha-glycoside of n-acetyl-4-epi-d-neuraminic acid (4-epi-neuac) is a substrate for sialidases from vibrio cholerae, clostridium perfringens, and arthrobacter ureafaciens, although to an extent which differs depending on the enzyme. surprisingly, v. cholerae sialidase is by far the slowest acting enzyme; this is in contrast to its usual behavior. fowl plague virus sialidase and bovine testis sialidase also cleave this glycoside ...	1988	3166979
metabolic products of microorganisms. 249. tetracenomycins b3 and d3, key intermediates of the elloramycin and tetracenomycin c biosynthesis.	tetracenomycins b3 and d3, besides tetracenomycin d (d1), were produced by a blocked mutant of the elloramycin producer streptomyces olivaceus tu 2353. the compounds were isolated as red powders, and their structures were elucidated by comparing their physicochemical data with those of the known tetracenomycins a2, b1, b2, d and e. tetracenomycin b3 (2), the main compound, and tetracenomycin d (3) were antibiotically inactive against gram-positive and gram-negative bacteria, whereas tetracenomyc ...	1988	3170342
synthetic ferrichrome analogues with growth promotion activity for arthrobacter flavescens.	two families of trihydroxamic acid analogues of ferrichrome were chemically synthesized and tested for biological activity with arthrobacter flavescens. compounds using a tertiary amine as anchor showed little activity. several compounds using tetrahedral carbon as anchor showed activity approaching or equalling that of the natural siderophore, ferrichrome. the biological activity is discussed in relation to physical and chemical properties of the analogues.	1988	3196346
are 'light-derepressible' genes controlled by metal-protein complexes?		1988	3072697
cometabolism of polychlorinated biphenyls: enhanced transformation of aroclor 1254 by growing bacterial cells.	acinetobacter sp. strain p6 and a soil isolate, arthrobacter sp. strain b1b, were tested for their ability to transform aroclor 1254 as washed resting cells and as growing cells with biphenyl as the substrate. growing cells were far superior to resting-cell suspensions in terms of total polychlorinated biphenyl (pcb) transformation, transformation of specific pcb congeners, and diversity of congeners that were attacked. growing cells of acinetobacter sp. strain p6 and arthrobacter sp. strain b1b ...	1988	3140725
oxidative side-chain and ring fission of pregnanes by arthrobacter simplex.	metabolic processes involving side-chain and ring cleavage of progesterone, 17-hydroxyprogesterone, 11-deoxycortisol and 16-dehydropregnenolone by arthrobacter simplex were studied. the formation of the metabolites from progesterone indicates a pathway somewhat different from normal in the enzymic reaction sequence, and the 17-hydroxyprogesterone metabolites reveal a non-enzymic rearrangement step. the presence of a hydroxy group at c-21, as in 11-deoxycortisol, induces reduction of the c-20 car ...	1988	3214423
structure of a ganglioside with cad blood group antigen activity.	the cad antigen is a rare erythrocyte blood group antigen expressed on both sialoglycoprotein and ganglioside structures. it is related both serologically and biochemically to the sda blood group antigen expressed on over 90% of caucasian erythrocytes. we reported previously that cad erythrocytes contain a novel ganglioside that binds helix pomatia lectin and inhibits human anti-sda antibody. we have now purified the cad ganglioside and determined its structure. the ganglioside contained glc-gal ...	1988	3167001
molecular cloning of the penicillin g acylase gene from arthrobacter viscosus.	penicillin g acylase was purified from the cultured filtrate of arthrobacter viscosus 8895gu and was found to consist of two distinct subunits with apparent molecular weights of 24,000 (alpha) and 60,000 (beta). the partial n-terminal amino acid sequences of the alpha and beta subunits were determined with a protein gas phase sequencer, and a 29-base oligonucleotide corresponding to the partial amino acid sequence of the alpha subunit was synthesized. an escherichia coli transformant having the ...	1988	3214149
siderophore production by salmonella typhi.	this study was initiated to determine the mechanism of iron-uptake in salmonella typhi. when stressed for iron, microorganisms produce siderophores to obtain the necessary nutrient. generally two types of siderophores exist: the phenolate-type predominantly produced by bacteria and the hydroxamate-type commonly secreted by fungi. results of this investigation showed that s. typhi produced siderophores of the phenolate-type since culture supernatant of the organism grown under iron-deprivation su ...	1988	2962581
iron acquisition by haemophilus influenzae.	the mechanisms for acquisition of iron by haemophilus influenzae and their role in pathogenesis are not known. heme and nonheme sources of iron were evaluated for their effect on growth of type b and nontypable strains of h. influenzae in an iron-restricted, defined medium. all 13 strains acquired iron from heme, hemoglobin, hemoglobin-haptoglobin, and heme-hemopexin. among nonheme sources of protein-bound iron, growth of h. influenzae was enhanced by partially saturated human transferrin but no ...	1988	2964410
[combined immobilized cultures producing fibrinolytic proteinases].	the authors obtained combined immobilised systems composed of a culture producing fibrinolytic proteinases and a stimulating strain. the optimal ratio between the two cultures in gel was selected at a high starting cell density. highly stable immobilised cultures were produced by growing the cells in gel particles. the interrelationship of the partners was studied in the binary immobilised culture. the biosynthetic activity of the system fell down to the level of a monoculture when the cells of ...	1988	2977814
nucleotide sequences of 5s ribosomal rnas of arthrobacter oxydans, arthrobacter polychromogenes, arthrobacter globiformis and 'brevibacterium helvolum'.		1988	2457874
dna probes with different specificities from a cloned 23s rrna gene of micrococcus luteus.	a 7500 bp psti restriction fragment of chromosomal dna from micrococcus luteus containing a 23s rrna gene was cloned in vector phe3 in e. coli rr 28 (the recombinant plasmid was designated par1). a recombinant phage (par5) hybridizing to all eubacteria tested was constructed by shotgun subcloning of the psti fragment in phage m13mp8. further subcloning of the fragments of the 23s rrna gene in the vectors ptz18r and ptz19r using selected restriction sites of the gene enabled us to select cloned f ...	1988	2462009
the sulfated polysaccharide-peptidoglycan complex potently inhibits embryonic angiogenesis and tumor growth in the presence of cortisone acetate.	in combination with cortisone acteate, the sulfated polysaccharide (sp)-peptidoglycan (pg) complex produced by an arthrobacter species was found to inhibit both embryonic angiogenesis of chick chorioallantoic membrane (cam) and growth of solid sarcoma 180 tumor in mice. three fractions obtained from the sp-pg complex by gel filtration showed a great difference in the inhibitory effect on angiogenesis of the cam, whereas in ex vivo study such a difference was reduced. of the three fractions, sp-p ...	1988	2463087
asni: a novel class ii restriction endonuclease from arthrobacter sp., strain n-cm, recognizing 5'-at/taat-3'.	a new class ii restriction endonuclease, asni, with a novel sequence specificity was isolated from the gram-positive eubacterium arthrobacter species, strain n-cm. asni recognizes the unambiguously defined palindromic hexanucleotide (formula: see text) consisting of a- and t-residues. the novel enzyme in the presence of mg2+ cleaves specifically both strands as indicated by the arrows. the staggered cuts generate 5'-protruding ends with single-stranded 5'-ta-3' dinucleotide extensions. the novel ...	1988	2841156
arthrobacter viscosus dna is modified at gatc sequence.	arthrobacter viscosus dna was resistance to digestion by restriction enzymes that are sensitive to methylation of the cytosine residue (but not of adenine) within the gatc recognition sequence. restriction enzymes sensitive to methylation of cytosine in other recognition sequences were not affected. a. viscosus dna thus appeared to contain methylated cytosine specifically at the gatc sequence.	1988	2844183
a host-vector system for an arthrobacter species.	an efficient host-vector system has been developed for an industrial strain of arthrobacter sp. (nrrl b3728)used for glucose isomerase production. protoplasts of arthrobacter were generated by treating the cells with 0.5 mg lysozyme ml(-1) for 60 min in a solution containing 0.5 m-sucrose. around 30% of the protoplasts regenerated on agar containing 0.5 m-sodium succinate as osmotic stabilizer. three hybrid vectors, pbl2100, pcg1100 and pcg2100, were constructed by combining the escherichia coli ...	1988	2846755
glycosaminoglycans in cortical autopsy samples from alzheimer brain.	each of the known classes of mammalian glycosaminoglycans, with the exception of keratan sulphate, was found in cerebral cortex samples from patients with alzheimer-type dementia and age-matched controls. these molecules were quantitated, after electrophoresis and staining with alcian blue dye, by scanning densitometry. no significant differences were found between the mean levels of each of the above glycosaminoglycans in frontal cortex from patients with dementia compared with controls. an inc ...	1988	3139840
[identification of the causative agent of allergic alveolitis in a selected group of patients by using microbiological and immunological methods].		1988	3043388
the phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: properties, mechanism, and regulation.	this review consists of three major sections. the first and largest section reviews the protein constituents and known properties of the phosphotransferase systems present in well-studied gram-positive bacteria. these bacteria include species of the following genera: (1) staphylococcus, (2) streptococcus, (3) bacillus, (4) lactobacillus, (5) clostridium, (6) arthrobacter, and (7) brochothrix. the properties of the different systems are compared. the second major section deals with the regulation ...	1988	3060316
[the structure of extracellular polysaccharide of arthrobacter globiformis].	an extracellular polysaccharide from arthrobacter globiformis is composed of n-acetyl-d-glucosamine, n-acetyl-d-fucosamine, 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid and o-acetyl groups in the ratio 1:1:2:1. on the basis of solvolysis with anhydrous hydrogen fluoride, which resulted in a tetrasaccharide fragment, and analysis by 1h and 13nmr spectroscopy, it was concluded that the polysaccharide has the following structure: (formula; see text).	1988	3219122
three dehalogenases and physiological restraints in the biodegradation of haloalkanes by arthrobacter sp. strain ha1.	arthrobacter sp. strain ha1 utilizes 18 c2-to-c8 1-haloalkanes for growth and synthesizes an inducible 1-bromoalkane debrominase of unknown physiological function (r. scholtz, t. leisinger, f. suter, and a.m. cook, j. bacteriol. 169:5016-5021, 1987) in addition to an inducible 1-chlorohexane halidohydrolase which dehalogenates some 50 substrates, including alpha, omega-dihaloalkanes. alpha, omega-dihaloalkanes were utilized by cultures of strain ha1 under certain conditions only. c9 and c8 homol ...	1988	3223767
enzymatic dehalogenation of 4-chlorobenzoate by extracts from arthrobacter sp. su dsm 20407.	in extracts from arthrobacter sp. su dsm 20407 an enzyme was detectable, that converted 4-chlorobenzoate into 4-hydroxybenzoate. this conversion was also observed when no oxygen was present in the reaction mixture. boiling for 5 min destroyed the enzyme activity. 4-bromo- and 4-iodobenzoate were substrates for the enzyme too, but not 4-fluorobenzoate, 4-chlorophenylacetate and 4-chlorocinnamic acid. the enzyme showed optimum activity at 16 degrees c and at ph 7-7.5. the specific activity in the ...	1988	3223988
degradation of 3-aminophenol by arthrobacter spec. ma3.	the bacterial strain ma3 capable of utilizing 3-aminophenol as the sole source of carbon, energy and nitrogen for growth was isolated from an enrichment culture and identified as an arthrobacter species. utilization of 0.68 mg/ml 3-aminophenol by batch cultures of this organism was characterized by a specific growth rate (mu) of 0.18 h-1 and a yield coefficient (y) of 0.60. in chemostat cultures of strain ma3 we determined a critical dilution rate (dc) of 0.175 h-1 by continuous addition of mine ...	1988	3236220
stereochemical studies of d-glucal hydration by alpha-glucosidases and exo-alpha-glucanases: indications of plastic and conserved phases in catalysis by glycosylases.	alpha-glucosidases from aspergillus niger, pig serum, ungerminated rice, buckwheat, and sugar beet seeds (but not from brewers' yeast or honeybee) were found to catalyze the hydration of d-glucal. each reactive alpha-glucosidase, incubated with d-glucal in d2o, was shown to protonate (deuteriate) this prochiral substrate from above its re face, i.e., from a direction opposite that assumed for protonating alpha-d-glucosidic substrates. at the same time, d-glucal hydration catalyzed by three of th ...	1988	3284583
use of polyethylene glycol-modified uricase (peg-uricase) to treat hyperuricemia in a patient with non-hodgkin lymphoma.	modification by covalent attachment of monomethoxypolyethylene glycol (peg) can reduce the immunogenicity and prolong the circulating life of injected enzymes, making their use as therapeutic agents feasible. we report the first clinical use of peg-modified arthrobacter protoformiae uricase (peg-uricase) to treat hyperuricemia in a patient with non-hodgkin lymphoma and renal insufficiency who was allergic to allopurinol. two intramuscular injections totaling 3 u/kg body weight during the first 3 ...	1988	3289428
neuraminidase induces capacitation and acrosome reaction in mammalian spermatozoa.	the treatment of epididymal spermatozoa of guinea pig and ejaculated spermatozoa of rabbit with neuraminidase from arthrobacter ureafaciens induced significant acrosome reaction while the neuraminidase from cl. perfringens failed to do so. the addition of the neuraminidase inhibitors kept the enzyme induced acrosome reaction to the control level. the zona-free hamster ova test showed that the treatment of spermatozoa with arthrobacter neuraminidase rendered 82% of the guinea pig and 69% of the r ...	1988	3351441
semisynthetic preparation of n-glycolylneuraminic acid containing gm1 ganglioside: chemical characterization, physico-chemical properties and some biochemical features.	n-glycolylneuraminic acid containing gm1, gm1(neugc), was prepared by semisynthetic procedure. the procedure makes use of gm1 ganglioside deacetylated at the level of sialic acid residue (deac-gm1) and of 1,3-dioxalan-2,4-dione. deac-gm1 is prepared from gm1 by alkaline hydrolysis in the presence of tetramethylammonium hydroxide and the glycolylating compound by reaction of glycolic acid with phosgene in dioxane, followed by cyclization under vacuum. mass spectrometric and nuclear magnetic reson ...	1988	3370722
novel mechanisms of resistance to lincosamides in staphylococcus and arthrobacter spp.	clinical isolates of staphylococcus and arthrobacter spp. were screened for lincosamide resistance. six different patterns of resistance were found. strains designated sf27 and sf28 showed low-level resistance to lincosamides: one was susceptible to erythromycin (sf27) and the other was resistant (sf28). analysis of ribosomes from the resistant strains in an in vitro poly(u)-dependent protein-synthesizing system showed that ribosomes of both strains were sensitive to lincomycin and clindamycin. ...	1988	3377455
human and bovine coronaviruses recognize sialic acid-containing receptors similar to those of influenza c viruses.	human coronavirus oc43 and bovine coronavirus elute from agglutinated chicken erythrocytes when incubated at 37 degrees c, suggesting the presence of a receptor-destroying enzyme. moreover, bovine coronavirus exhibits an acetylesterase activity in vitro using bovine submaxillary mucin as substrate similar to the enzymatic activity found in influenza c viruses. furthermore, pretreatment of erythrocytes with either influenza c virus or bovine coronavirus eliminates subsequent binding and agglutina ...	1988	3380803
induction of a light-inducible gene in arthrobacter sp. by exposure of cells to chelating agents and ph 5.	transcription of a light-inducible gene in the prokaryote arthrobacter sp. is induced in the dark when cells are incubated with chelating agents or in medium at ph 5. however, repletion of metal ions such as ca2+, mn2+ or zn2+ or an increase in ph is required for accumulation of the gene product, an mr 21,000 polypeptide. but such changes in condition restore repression of the gene, and the decay in the rate of synthesis of the polypeptide follows the same time-course as when photodynamically in ...	1988	3382667
oxidative pathway from squalene to geranylacetone in arthrobacter sp. strain y-11.	the reaction pathway from squalene to trans-geranylacetone in arthrobacter sp. strain y-11 was studied. the enzyme or enzymes catalyzing squalene degradation were found to be membrane bound. stoichiometric analysis of a cell-free system revealed that the ratio of squalene to trans-geranylacetone changed from 1:2 to 1:1 as the reaction proceeded, indicating two steps in geranylacetone formation. the initial step was found to be oxygenase catalyzed, from the absolute requirement for molecular oxyg ...	1988	16347551
degradation of the phosphonate herbicide glyphosate by arthrobacter atrocyaneus atcc 13752.	of nine authentic arthrobacter strains tested, only a. atrocyaneus atcc 13752 was capable of using the herbicide glyphosate [n-(phosphonomethyl)glycine] as its sole source of phosphorus. contrary to the previously isolated arthrobacter sp. strain glp-1, which degrades glyphosate via sarcosine, a. atrocyaneus metabolized glyphosate to aminomethylphosphonic acid. the carbon of aminomethylphosphonic acid was entirely converted to co(2). this is the first report on glyphosate degradation by a bacter ...	1988	16347639
isolation and preliminary characterization of twenty bacteriophages infecting either brevibacterium or arthrobacter strains.	thirty-seven bacteriophages plaquing on corynebacterium, brevibacterium, or arthrobacter strains were isolated from soil or vegetation samples. restriction analysis of phage dna indicated that 20 phages were unique; one of them produced entirely turbid plaques on brevibacterium ketoglutamicum and was characterized as temperate. all these phages were assigned to group b of the classification of bradley (bacteriol. rev. 31:230-314, 1967) and had relatively narrow host ranges.	1988	16347657
mineralization of carbofuran by a soil bacterium.	a bacterium, tentatively identified as an arthrobacter sp., was isolated from flooded soil that was incubated at 35 degrees c and repeatedly treated with carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranyl n-methylcarbamate). this bacterium exhibited an exceptional capacity to completely mineralize the ring-labeled c in carbofuran to co(2) within 72 to 120 h in a mineral salts medium as a sole source of carbon and nitrogen under aerobic conditions. mineralization was more rapid at 35 degrees c t ...	1988	16347722
isolation and characterization of a mutant of arthrobacter sp. strain glp-1 which utilizes the herbicide glyphosate as its sole source of phosphorus and nitrogen.	arthrobacter sp. strain glp-1, grown on glucose as a carbon source, utilizes the herbicide glyphosate [n-(phosphonomethyl)glycine] as its sole source of phosphorus as well as its sole source of nitrogen. the mutant strain glp-1/nit-1 utilizes glyphosate as its sole source of nitrogen as well. in strain glp-1, p(i) was a potent competitive inhibitor of glyphosate uptake (k(i), 24 mum), while the affinity of p(i) for the uptake system of strain glp-1/nit-1 was reduced by 2 orders of magnitude (k(i ...	1988	16347784
characterization of 15 selected coccal bacteria isolated from antarctic rock and soil samples from the mcmurdo-dry valleys (south-victoria land).	approximately 1500 cultures of microorganisms were isolated from rocks and soils of the ross desert (mcmurdo-dry valleys). from these, 15 coccoid strains were chosen for more detailed investigation. they were characterized by morphological, physiological and chemotaxonomical properties. all isolates were gram-positive, catalase-positive and nonmotile. six strains showed red pigmentation and could be identified as members of the genera micrococcus (m. roseus, m. agilis) or deinococcus. in sp ...	1988	11538335
microbiological degradation of malodorous substances of swine waste under aerobic conditions.	phenol, p-cresol, and volatile fatty acids (vfa; acetic, propionic, isobutyric, butyric, isovaleric, and valeric acids) were used as odor indicators of swine waste. aeration of the waste allowed the indigenous microorganisms to grow and degrade these malodorous substances. the time required for degradation of these substances varied according to the waste used, and it was not necessarily related to their concentrations. using a minimal medium which contained one of the malodorous compounds as so ...	1987	16347254
uptake of glyphosate by an arthrobacter sp.	the uptake of glyphosate (n-[phosphonomethyl]glycine) by an arthrobacter sp. which can utilize this herbicide as its sole source of phosphorus was investigated. orthophosphate suppressed the expression of the uptake system for glyphosate and also competed with glyphosate for uptake. the k(m) for glyphosate uptake was 125 mum, and the k(i) for orthophosphate was 24 mum. organophosphonates as well as organophosphates inhibited glyphosate uptake, but only organophosphates and orthophosphate suppres ...	1987	16347356
bacterial flora in bottled uncarbonated mineral drinking water.	a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...	1987	3446349
comparison of backbone structures of glucose isomerase from streptomyces and arthrobacter.	the c alpha backbones of the glucose isomerase molecules of streptomyces rubiginosus and arthrobacter have been determined by x-ray crystallography and compared. each molecule is a tetramer of eight-stranded alpha/beta barrels, and the mode of association of the tetramers is identical in each case. the arthrobacter electron density shows four additional amino acids at the carboxyl terminus. there is also an insertion of six amino acids at position 277, and two individual insertions at about posi ...	1987	3508294
microbiological and chemical dehydrogenation of withaferin a.	arthrobacter simplex dehydrogenated withaferin a to 4-dehydrowithaferin a but it was not able to dehydrogenate this substrate in position 27. 27-dehydrowithaferin a was prepared chemically using pyridinium chlorochromate. whereas 4-dehydrowithaferin a surpassed in its effect on leukemic (388 cells the original compound and all its derivates synthesized so far, 27-dehydrowithaferin a was biologically inactive.	1987	3583136
isolation and characterization of an s-ethyl-n,n-dipropylthiocarbamate-degrading arthrobacter strain and evidence for plasmid-associated s-ethyl-n,n-dipropylthiocarbamate degradation.	arthrobacter sp. strain te1 isolated from s-ethyl-n,n-dipropylthiocarbamate (eptc)-exposed soil degraded this herbicide effectively and could grow on eptc as the sole carbon source. te1 harboured four plasmids of 65.5, 60, 50.5, and 2.5 megadaltons. spontaneous mutants unable to degrade eptc arose at a high frequency, and this was further increased by treatment of the culture with acridine orange or incubation at high temperature. all eptc degradation-deficient (e-) mutants lacked the 50.5-megad ...	1987	3606092
[microbial transformation of 16 alpha-methyl-3 beta, 17 alpha-dihydroxy-5 alpha-pregnane-20-one-3 acetate].		1987	3618239
6-hydroxy-d-nicotine oxidase of arthrobacter oxidans. gene structure of the flavoenzyme and its relationship to 6-hydroxy-l-nicotine oxidase.	the nucleotide sequence of the 6-hydroxy-d-nicotine oxidase (6-hdno) gene of arthrobacter oxidans is presented. this covalently flavinylated enzyme specifically oxidizes 6-hydroxy-d-nicotine to 6-hydroxy-n-methylmyosmine. coinduced in the presence of nicotine is a 6-hydroxy-l-nicotine-specific enzyme, 6-hydroxy-l-nicotine oxidase (6-hlno), with fad noncovalently bound to the apoprotein. a comparison of the nucleotide-derived amino acid sequence of the 6-hdno with the amino acid sequence data obt ...	1987	3622516
[kinetics of delta'-dehydrogenation of hydrocortisone by arthrobacter simplex by-2-13. i. kinetics of delta'-dehydrogenation of hydrocortisone by free cells of arthrobacter simplex by-2-13].		1987	3630138
[microbial reduction of c20-keto of corticosteroids by arthrobacter 9-2. ii. bioconversion of 3 beta,17 alpha,21-triol-5 alpha-pregnan-3-one].		1987	3630139
[kinetics of delta'-dehydrogenation of hydrocortisone by arthrobacter simplex by-2-13. ii. kinetics of delta'-dehydrogenation of hydrocortisone by immobilized a. simplex by-2-13].		1987	3630142
immobilization of living microbial cells in polyacrylamide gel.		1987	3298943
maintenance and stability of introduced genotypes in groundwater aquifer material.	three indigenous groundwater bacterial strains and pseudomonas putida harboring plasmids tol (pwwo) and rk2 were introduced into experimentally contaminated groundwater aquifer microcosms. maintenance of the introduced genotypes was measured over time by colony hybridization with gene probes of various specificity. on the basis of the results of colony hybridization quantitation of the introduced organisms and genes, all introduced genotypes were stably maintained at approximately 10(5) positive ...	1987	3300546
[immobilized systems producing fibrinolytic proteinases].		1987	3319470
phylogenetic analysis of the coryneform bacteria by 5s rrna sequences.	nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ...	1987	3106318
metabolism of endogenous trehalose by streptomyces griseus spores and by spores or cells of other actinomycetes.	the disaccharide trehalose is accumulated as a storage product by spores of streptomyces griseus. nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. in contrast, spores rapidly depleted their trehalose pools during the first hours of germination. extracts of dormant spores contained a high specific activity of the enzyme trehalase. the level of trehalase remained relatively constant during germination or incubation in buffer. nongerminating sp ...	1987	3117770
integration of bioconversions and downstream processing. some model studies.		1987	3124693