Publications

[plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of arthrobacter].	arthrobacter crysallopoietes strain km-4 degrading 2,6-dimethylpyridine and strain km-4a degrading both 2,6-dimethylpyridine and pyridine, arthrobacter sp. km-4b degrading 2,4-dimethylpyridine were isolated from soil. arthrobacter crystallopoietes km-4 and arthrobacter sp. km-4b contain 100 md plasmids pbs320 and pbs323. arthrobacter crystallopietes km-4a harbours a 100 md and 80 md plasmids. plasmid curing and conjugation transfer results confirm that these plasmids are involved in degradation ...	1992	1454076
[effect of normal and specific immune sera on neuraminidase activity].	we have got evidence that there is no antigenic relationship reflecting the structural similarity between neuraminidases synthesized by noncholera vibrios and arthrobacter nicotianae. the cross-reactions between the enzymes and heterological antisera were not observed. antibodies against the a. nicotianae neuraminidase inhibited the activity of the enzyme for a glycomacropeptide of milk whey and for components of the blood serum, and had no effect no the neuraminidase from noncholera vibrios. an ...	1992	1528820
indole-3-acetic acid (iaa) production by arthrobacter species isolated from azolla.	arthrobacter species, isolated from the leaf cavities and the microsporocarps of the aquatic fern species azolla pinnata and azolla filiculoides, produced indole-3-acetic acid (iaa) in culture when the precursor tryptophan was added to the medium. no iaa production was detected in the absence of tryptophan. maximum iaa formation was obtained in the first 2 d of incubation. part of the tryptophan was transformed to n alpha-acetyl-l-tryptophan.	1992	1564446
bacteria that degrade p-chlorophenol isolated from a continuous culture system.	two gram-positive coryneform bacteria that degraded p-chlorophenol isolated from a continuous culture system are characterized. isolate b (probably and arthrobacter sp.) completely removed the p-chlorophenol from a medium with a concomitant increase in cell density within 16 h. isolate f similarly removed the p-chlorophenol within 28 h but without an increase in cell density. isolates b and f also removed the p-chlorophenol from a medium with p-chlorophenol as the sole carbon source within 32 an ...	1992	1581863
mechanism of d-fructose isomerization by arthrobacter d-xylose isomerase.	the mechanism of d-fructose isomerization by arthrobacter d-xylose isomerase suggested from x-ray-crystallographic studies was tested by detailed kinetic analysis of the enzyme with various metal ions at different ph values and temperatures. at d-fructose concentrations used in commercial processes mg2+ is the best activator with an apparent dissociation constant of 63 microm; co2+ and mn2+ bind more strongly (apparent kd 20 microm and 10 microm respectively) but give less activity (45% and 8% r ...	1992	1567370
levels of trehalose and glycogen in arthrobacter globiformis under conditions of nutrient starvation and osmotic stress.	cells of arthrobacter globiformis grown in carbohydrate-rich media were found to contain large quantities of low-mr carbohydrates (800 micrograms/mg protein) and only small amounts of amino acids, in addition to high amounts of glycogen (2 mg/mg protein). at increasing osmotic values of the medium, low-mr carbohydrate levels increased to 1300 micrograms/mg protein. low-mr pools were extracted from the cells with hot 75% ethanol, and subjected to thin layer, gel and gas-liquid chromatography. the ...	1992	1575469
reclassification of two strains of arthrobacter oxydans and proposal of arthrobacter nicotinovorans sp. nov.	arthrobacter oxydans dsm 419 and dsm 420 have chemical and microbiological properties that are consistent with assignment to the genus arthrobacter. both organisms have the lysine-alanine-threonine-alanine peptidoglycan type. dna-dna pairing studies indicated that a. oxydans dsm 419 should be reclassified as arthrobacter ureafaciens and that a. oxydans dsm 420t forms the nucleus of a distinct genomic species. we propose that a. oxydans dsm 420 should be reclassified as arthrobacter nicotinovoran ...	1992	1581183
molecular mechanics simulations of a conformational rearrangement of d-xylose in the active site of d-xylose isomerase.	a proposed reaction mechanism for the enzyme d-xylose isomerase involves the ring opening of the cyclic substrate with a subsequent conformational rearrangement to an extended open-chain form. restrained energy minimization was used to simulate the rearrangement. in the ring-opening step, the substrate energy function was gradually altered from a cyclic to an open-chain form, with energy minimization after each change. the protein/sugar contact energy did not increase significantly during the pr ...	1992	1620692
hemicellulose bioconversion to polyanionic heteropolysaccharides.	anionic polysaccharides, traditionally obtained from plant or algal sources, have a variety of commercial uses. such gums from microorganisms have received increased recent interest. we have initiated a program to investigate the bioconversion of pentosans to rheologically useful anionic extracellular polysaccharides (aeps). a number of earlier-described species, including cryptococcus laurentii, klebsiella pneumoniae, arthrobacter viscosus, and pseudomonas atcc 31260, appear to have potential i ...	1992	1622201
effect of bacterial extracellular polymers on the saturated hydraulic conductivity of sand columns.	columns were packed with clean quartz sand, sterilized, and inoculated with different strains of bacteria, which multiplied within the sand at the expense of a continuous supply of fresh nutrient medium. the saturated hydraulic conductivity (hcsat) of the sand was monitored over time. among the four bacterial strains tested, one formed a capsule, one produced slime layers, and two did not produce any detectable exopolymers. the last two strains were nonmucoid variants of the first two. only one ...	1992	1622240
interaction of the regulatory protein nicr1 with the promoter region of the pao1-encoded 6-hydroxy-d-nicotine oxidase gene of arthrobacter oxidans.	the d,l-nicotine catabolism of the gram-positive soil bacterium arthrobacter oxidans is linked to the presence within the cells of the 160 kb catabolic plasmid pao1. pao1-cured cells lost the catabolic enzymes and reintroduction of pao1 by electroporation into cured cells reestablished the nic+ phenotype. dna band shift assays with extracts from cured and pao1+ cells suggested that pao1 encodes the regulatory protein nicr1. footprint analysis revealed that two homologous palindromes (ir1 and ir2 ...	1992	1630318
structural study of cell wall mannan of a candida albicans (serotype a) strain.	the structure of the mannan of candida albicans nih a-207 strain (serotype a) was investigated by adopting mild acetolysis followed by enzymolysis with an arthrobacter gjm-1 exo-alpha-mannosidase. the resultant oligosaccharides, from pentaose to octaose (where manp = d-mannopyranose), were identified as manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp, manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)- manp alpha (1----2)manp, manp beta (1- ...	1992	1368047
action of levan fructotransferase of arthrobacter ureafaciens on a mixture of branched levanpentasaccharides.		1992	1368345
multi-recognition capability of e-selectin in a dynamic flow system, as evidenced by differential effects of sialidases and anti-carbohydrate antibodies on selectin-mediated cell adhesion at low vs. high wall shear stress: a preliminary note.	e-selectin has a "multi-recognition" capability in terms of epitope binding specificity, depending on adhesion conditions (static vs. low- or high-shear stress dynamic systems). specifically, (i) adhesion based on expression of alpha 2-->3 sialylated le(x) (sle(x)) is prominent under static or low shear stress dynamic conditions; (ii) adhesion under high shear stress dynamic conditions does not depend on the known sle(x) species, but rather on lex with an adjacent unidentified sialosyl substitut ...	1992	1282810
[cloning and expression of the arthrobacter globiformis fcb genes in bacillus subtilis].	the fcb genes of arthrobacter globiformis kzt1 coding for the dehalogenase (4-chlorobenzoate-4-hydroxylase) activity have been cloned. the characteristics of fcb genes expression have been studied. the recombinant strains of bacillus subtilis 6jm15 (pcbs 311) and 6jm15 (pcbs1) have shown the decreased level of substrate dehalogenation as compared with the one in the parent strain kzt1 and the recombinant strains of escherichia coli and pseudomonas putida.	1992	1301499
effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes.	tetralin is toxic to bacterial cells at concentrations below 100 mumol/liter. to assess the inhibitory action of tetralin on bacterial membranes, a membrane model system, consisting of proteoliposomes in which beef heart cytochrome c oxidase was reconstituted as the proton motive force-generating mechanism, and several gram-positive and gram-negative bacteria were studied. because of its hydrophobicity, tetralin partitioned into lipid membranes preferentially (lipid/buffer partition coefficient ...	1992	1314806
acsi, a new restriction endonuclease from arthrobacter citreus 310 recognizing 5'-pu decreases aattpy-3'.		1992	1322532
taxonomic study of the genus brachybacterium: brachybacterium nesterenkovii sp. nov.	a new species, brachybacterium nesterenkovii, is proposed for a group of coryneform bacteria that were isolated from milk products. these organisms have morphological, biochemical, and chemotaxonomic characteristics that are peculiar to the genus brachybacterium. in contrast to strains of the only previously described species of the genus, brachybacterium faecium, the representatives of the new species lack glycine in their peptidoglycan, although the peptidoglycan is of the same general type, a ...	1992	1736971
apoi, a unique restriction endonuclease from arthrobacter protophormiae which recognizes 5' raatty 3'.		1992	1614876
myceloid cell formation in arthrobacter globiformis during osmotic stress.	arthrobacter globiformis was grown in a semi-defined liquid medium containing added solutes to determine the effects of osmotic stress on its reproduction and cell morphology. there was a progressive reduction in the specific growth rate during exponential phase as the concentration of nacl was increased, although the final yields of the cultures during stationary phase were not affected. clusters of branching myceloid cells rather than the typical bacillary forms predominated during exponential ...	1992	1644706
activity and stability of an entrapped-cell system for the delta(1)-dehydrogenation of steroids in organic media.	whole arthrobacter simplex cells entrapped in kappa-carra-geenan or in two types of polyurethane foam, or adsorbed on silanized celite, were tested for the delta(1)-dehydrogenation of hydrocortisone and its derivatives in organic media. catalytic activity and stability levels were evaluated for the immobilized cells in buffer with 2.5% (vol/vol) methanol, and in three buffer-saturated solvents (n-octan-1-ol, n-decan-1-ol, and chloroform). the addition of glutamate to the immobilization support s ...	1992	18601223
effect of adsorbents on degradation of toxic organic compounds by coimmobilized systems.	the use of coimmobilized systems for treatment of toxic organic compounds has been proposed. the proposed approach combines the use of adsorbents and laboratory identified microorganisms immobilized in a protective permeable barrier to achieve a greater degree of control over the remediation process. this study was launched to understand the effect of adsorbents and changes in adsorption on the degradation of toxic compounds by coimmobilized systems. the specific case studied involved the degrad ...	1992	18600991
competition for ammonium between nitrifying and heterotrophic bacteria in continuously percolated soil columns.	although the absence of nitrate formation in grassland soils rich in organic matter has often been reported, low numbers of nitrifying bacteria are still found in these soils. to obtain more insight into these observations, we studied the competition for limiting amounts of ammonium between the chemolithotrophic ammonium-oxidizing species nitrosomonas europaea and the heterotrophic species arthrobacter globiformis in the presence of nitrobacter winogradskyi with soil columns containing calcareou ...	1992	16348787
heteropolysaccharide formation by arthrobacter viscosus grown on xylose and xylose oligosaccharides.	arthrobacter viscosus nrrl b-1973 produces its viscous extracellular polysaccharides (eps) when grown on media containing xylose or enzymatic xylan hydrolysates. crude eps formation from xylose averaged 12 g/liter when initial culture ph was adjusted to 8.0 and total nitrogen was limited to 0.03%. purified eps from pentose and hexose substrates were analyzed for their monosaccharide, acetyl, and uronic acid components, intrinsic viscosities, and average molecular masses. differences were apparen ...	1992	16348800
scanning electron and light microscopic study of microbial succession on bethlehem st. nectaire cheese.	st. nectaire cheese is a semisoft cheese of french origin that, along with brie and camembert cheeses, belongs to the class of surface mold-ripened cheese. the surface microorganisms that develop on the cheese rind during ripening impart a distinctive aroma and flavor to this class of cheese. we have documented the sequential appearance of microorganisms on the cheese rind and in the curd over a 60-day ripening period. scanning electron microscopy was used to visualize the development of surface ...	1992	16348797
effects of grazing by flagellates on competition for ammonium between nitrifying and heterotrophic bacteria in chemostats.	the enhanced mineralization of organic nitrogen by bacteriophagous protozoa is thought to favor the nitrification process in soils, in which nitrifying bacteria have to compete with heterotrophic bacteria for the available ammonium. to obtain more insight into this process, the influence of grazing by the bacteriovorous flagellate adriamonas peritocrescens on the competition for limiting amounts of ammonium between the ammonium-oxidizing species nitrosomonas europaea and the heterotrophic specie ...	1992	16348722
relationship between transport of bacteria and their clogging efficiency in sand columns.	in an earlier article, we reported that, under conditions in which neither exopolymers nor bacterial mats were produced, arthrobacter sp. strain ak19 was an effective plugging agent in sand columns, whereas the bacterial strain sli had no significant effect on the permeability of the medium. a laboratory experiment with sand columns was carried out to elucidate the causes of this difference in behavior. measured values of the saturated hydraulic conductivity of the sand were explained in terms o ...	1992	16348753
relationship between cell surface properties and transport of bacteria through soil.	a study was conducted to relate the properties of enterobacter, pseudomonas, bacillus, achromobacter, flavobacterium, and arthrobacter strains to their transport with water moving through soil. the bacteria differed markedly in their extent of transport; their hydrophobicity, as measured by adherence to n-octane and by hydrophobic-interaction chromatography; and their net surface electrostatic charge, as determined by electrostatic interaction chromatography and by measurements of the zeta poten ...	1991	16348394
competition for ammonium between nitrifying and heterotrophic bacteria in dual energy-limited chemostats.	the absence of nitrification in soils rich in organic matter has often been reported. therefore, competition for limiting amounts of ammonium between the chemolithotrophic ammonium-oxidizing species nitrosomonas europaea and the heterotrophic species arthrobacter globiformis was studied in the presence of nitrobacter winogradskyi in continuous cultures at dilution rates of 0.004 and 0.01 h. ammonium limitation of a. globiformis was achieved by increasing the glucose concentration in the reservoi ...	1991	16348588
biodegradation by an arthrobacter species of hydrocarbons partitioned into an organic solvent.	an arthrobacter strain mineralized naphthalene and n-hexadecane dissolved in 2,2,4,4,6,8,8-heptamethylnonane. the extent of mineralization increased with greater volumes of solvent. measurements under aseptic conditions of the partitioning of naphthalene into the aqueous phase from the solid phase or from heptamethylnonane showed that the rates were rapid and did not limit mineralization. the rate of mineralization of hexadecane was rapid, although partitioning of the compound into aqueous solut ...	1991	16348485
effects of solvent molecular toxicity and microenvironment composition on the delta(1) dehydrogenataon activity of arthrobacter simplex cells.	solvent selection tests were carried out for the delta(1) dehydrogenation of 6-alpha-methylhydrocortisone-21-acetate by arthrobacter simplex cells in the presence of organic solvents. solubility limits were determined for substrate and product in dry and water-saturated solvents and solvent mixtures. molecular toxicity levels were estimated by measuring the dehydrogenation activity decay of freely suspended cells incubated in solvent-saturated aqueous media. chloroform and n-decan-1-ol were the ...	1991	18597345
steroid bioconversion in a microemulsion system.	the delta(1,2)-dehydrogenation of high concentrations of the steroid -methyl-reichstein's compound s-21-acetate (16mrsa) in a microemulsion system was studied using heat-dried and thawed arthrobacter simplex cells as biocatalyst. the microemulsion system consists of an organic phase [75-95% (v/v)] with steroid (1-60 g/l(tot)), an aqueous phase [5-25% (v/v)] containing the cells (5-30 g/l(tot)), and a neutral surfactant (5-20 g/l organic solvent). benzene derivatives, which solubilize 16mrsa up t ...	1991	18600717
ultrasound-enhanced bioprocess. ii: dehydrogenation of hydrocortisone by arthrobacter simplex.	dehydrogenation of hydrocortisone by agitated suspensions of free and immobilized cells of arthrobacter simplex atcc 6946 was investigated under controlled ultrasonic irradiation at a frequency of 20 kh(z). the microbial conversion was optimized first with respect to mechanical agitation and subsequently with respect to an additionally superimposed sonication. the optimization of ultrasound intensity and its mode of application were established at a level which maintained the structural integrit ...	1991	18597329
transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.	endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae has transglycosylation activity. treatment of (man)6(glcnac)2asn with the enzyme in the presence of free n-acetylglucosamine gave a mixture of (man)6glcnac and (man)6glcnac beta 1----4glcnac mixture. n-acetylglucosamine at the reducing end of the latter sugar chain was found by hplc of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 mhz 1h-nmr spec ...	1991	1776953
the combination of a bacterial polysaccharide and tamoxifen inhibits angiogenesis and tumour growth.		1991	1713945
phylogeny of the whipple's-disease-associated bacterium.	efforts to culture and identify the intracellular bacteria associated with whipple's disease have been unsuccessful. nucleotide sequencing and amplification by the polymerase chain reaction was done on the bacterial 16 s ribosomal dna present in a small-bowel biopsy specimen taken from a patient with whipple's disease. a search by computer for similar rrna sequences filed in databases showed the whipple's-associated organism to be most similar to bacteria of the rhodococcus, streptomyces, and ar ...	1991	1714530
the breast tumor-associated epitope defined by monoclonal antibody 3e1.2 is an o-linked mucin carbohydrate containing n-glycolylneuraminic acid.	the breast cancer-associated epitope (mammary serum antigen or msa) defined by monoclonal antibody (mab) 3e1.2 is a neuraminidase-sensitive carbohydrate expressed on muc-1-encoded molecules. however, the reactivity of mab 3e1.2 is also reduced by protease treatment of the mucin, which suggests that 3e1.2 binds to multimers of the sialylated carbohydrate in a protein conformation-dependent manner. the common n-acetyl derivative of neuraminic acid (5-acetylneuraminic acid) is not involved in the e ...	1991	1718585
delta'-dehydrogenation of steroids by arthrobacter simplex immobilized in calcium polygalacturonate beads.	arthrobacter simplex atcc 6946 (viable cells) was immobilized in a calcium polygalacturonate gel. the trapped cells were used for repeated batchwise bioconversion of steroids. reichstein's compound s and hydrocortisone were dehydrogenated introducing a double bond between c1 and c2 of ring a. the products 1-dehydro s and prednisolone, respectively, were identified by high pressure liquid chromatography. steroid dehydrogenase activity increased in the system when an artificial electron acceptor, ...	1991	1367800
3,4-dihydroxyxanthone dioxygenase from arthrobacter sp. strain gfb100.	bacterial extradiol ring-fission dioxygenases play a critical role in the transformation of multiring aromatic compounds to more readily biodegradable aromatic or aliphatic intermediates. arthrobacter sp. strain gfb100 utilizes an extradiol meta-fission dioxygenase, 3,4-dihydroxyxanthone dioxygenase (dhxd), in the catabolism of the three-ring oxygen heterocyclic compound xanthone. in this paper, we show that dhxd is a cytosolic enzyme, induced by growth on xanthone and maximally expressed during ...	1991	1768091
metabolism of carbamate insecticides by resting cells and cell-free preparations of a soil bacterium, arthrobacter sp.		1991	2032002
occurrence of two structural types of mercury reductases among gram-positive bacteria.	structural variants of mercury reductase containing the n-terminal domain, which is easily cleaved by trypsin, have been found in gram-positive bacteria with a low genomic g + c content (bacillus, staphylococcus and, possibly, some other genera). mercury reductases without the n-terminal domain and relatively resistant to limited proteolysis are typical for gram-positive bacteria with a high genomic g + c content (arthrobacter, citreobacterium, micrococcus, mycobacterium, rhodococcus). both type ...	1991	2040434
biodegradation of refractory hydrocarbon biomarkers from petroleum under laboratory conditions.	biomarkers are of great value in petroleum exploration because they provide essential information about the geological history of oils and source rocks. steranes are of particular importance as they can be related to naturally occurring precursors. these compounds generally experience intense biodegradation, however, which alters their original distribution and obscures the information that they carry regarding oil maturity and source material. in an attempt to identify the microorganisms respon ...	1991	2052089
description of the erythromycin-producing bacterium arthrobacter sp. strain nrrl b-3381 as aeromicrobium erythreum gen. nov., sp. nov.	arthrobacter sp. strain nrrl b-3381t (t = type strain) is a nonmycelial, nonsporulating actinomycete that produces the macrolide antibiotic erythromycin. this bacterium differs in many ways from the type species of the genus arthrobacter (arthrobacter globiformis), suggesting that a taxonomic revision is appropriate. the g + c content of strain nrrl b-3381t dna is 71 to 73 mol%, and the peptidoglycan of this organism contains ll-diaminopimelic acid. evolutionary distance data obtained from 16s r ...	1991	1883712
d-xylose (d-glucose) isomerase from arthrobacter strain n.r.r.l. b3728. gene cloning, sequence and expression.	arthrobacter strain n.r.r.l. b3728 superproduces a d-xylose isomerase that is also a useful industrial d-glucose isomerase. the gene (xyla) that encodes it has been cloned by complementing a xyla mutant of the ancestral strain, with the use of a shuttle vector. the 5' region shows strong sequence similarity to escherichia coli consensus promoters and ribosome-binding sequences and allows high levels of expression in e. coli. the coding sequence shows similarity to those for other d-xylose isomer ...	1991	1854339
d-xylose (d-glucose) isomerase from arthrobacter strain n.r.r.l. b3728. purification and properties.	d-xylose (d-glucose) isomerase was purified to homogeneity in yields of approx. 1 g/kg of wet cells from a strain of arthrobacter that produces it as about 10% of total soluble protein. it is a tetramer of identical 43,114 da subunits containing a preponderance of acidic residues and no cysteine. partial protein sequences were determined as a step to gene cloning. it requires mg2+, co2+ or mn2+ for activity, mg2+ being best; ca2+ is an inhibitor, competitive with mg2+. it is a good d-glucose iso ...	1991	1854338
substrate interactions of benzene, toluene, and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries.	benzene, toluene, and p-xylene (btx) were degraded by indigenous mixed cultures in sandy aquifer material and by two pure cultures isolated from the same site. although btx compounds have a similar chemical structure, the fate of individual btx compounds differed when the compounds were fed to each pure culture and mixed culture aquifer slurries. the identification of substrate interactions aided the understanding of this behavior. beneficial substrate interactions included enhanced degradation ...	1991	1746958
structural organization of the corynebacterium glutamicum plasmid pcg100.	pcg100, a 3 kb cryptic plasmid of corynebacterium glutamicum atcc 13058, probably identical with psr1 from c. glutamicum atcc 19223, was characterized. the minimum region for autonomous replication was shown to be contained on a 1.9 kb bglii-ncoi fragment; a 380 bp hindiii-sphi fragment can replicate in the presence of the parental plasmid, which presumably provides a trans-acting replication factor. derivatives of pcg100 are able to replicate in several corynebacterium, brevibacterium and arthr ...	1991	1748866
copper in biological systems. a report from the 6th manziana conference, september 23-27, 1990.	enzymes and proteins: ao, amine oxidase; and as proposed in reference 3, bsao, bovine serum ao; ssao, swine serum ao; skdao, swine kidney ao; psao, pea seedling ao; apao, arthrobacter p1ao; madh, methylamine dehydrogenase; aao, ascorbic acid oxidase; alpha-ae, alpha-amidating enzyme; az, azurin; cox, cytochrome c oxidase; cp, ceruloplasmin; dbh, dopamine beta-hydroxylase; go, galactose oxidase; hc, hemocyanin; mt, metallotheonein; nir, nitrite reductase; sod, superoxide dismutase. cofactors: dop ...	1991	1757786
enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.	the transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. when (man)6(glcnac)2asn was used as a substrate for the transglycosylation, (man)6glcnac-glc, (man)6glcnac-man, (man)6glcnac-chitobiose, and (man)6glcnac-gentiobiose were synthesized. their structures were identified by hplc, ion spray mass spectrometry, and digestion with glycosidases. endo-beta-n-acetylglucosaminidases hydrolyzed t ...	1991	1804102
l-lysine production by s-2-aminoethyl-l-cysteine-resistant mutants of arthrobacter globiformis.	using mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing arthrobacter globiformis excreting l-lysine in good amounts. for further improvement, mutants resistant to the lysine analog s-(2-aminoethyl)-l-cysteine have been isolated from homoserine auxotrophs. for the three potent mutants tested, white's medium was found to be the best. glucose, ammonium nitrate and biotin were found to be optimum at 280 mmol/l, 40 m ...	1991	1841850
isolation method for lysine-excreting mutants of arthrobacter globiformis.	to improve the yield of lysine by the isolate, auxotrophic mutants were isolated. among the mutants, only one auxotrophic mutant required vitamin b12. this mutant produced alpha-alanine. about 200 mutants resistant to the lysine analog s-(2-aminoethyl)-l-cysteine were isolated and some of them produced well above the wild type.	1991	1841851
determination of glycosylation sites using a protein sequencer and deglycosylation of native yeast invertase by endo-beta-n-acetylglucosaminidase.	endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae was tested for its capacity to release n-linked sugar chains from native yeast invertase. the enzyme liberated about 80% of the sugar chains from the native invertase. deglycosylated invertase was digested by chymotrypsin or pepsin, and twelve n-acetylglucosamine-containing glycopeptides were isolated. the amino acid sequences of these glycopeptides were analyzed by a protein sequencer, and the elution position of 4-l-aspartylglyc ...	1991	1805802
purification and characterization of haloalcohol dehalogenase from arthrobacter sp. strain ad2.	an enzyme capable of dehalogenating vicinal haloalcohols to their corresponding epoxides was purified from the 3-chloro-1,2-propanediol-utilizing bacterium arthrobacter sp. strain ad2. the inducible haloalcohol dehalogenase converted 1,3-dichloro-2-propanol, 3-chloro-1,2-propanediol, 1-chloro-2-propanol, and their brominated analogs, 2-bromoethanol, as well as chloroacetone and 1,3-dichloroacetone. the enzyme possessed no activity for epichlorohydrin (3-chloro-1,2-epoxypropane) or 2,3-dichloro-1 ...	1991	1846134
a cu(i)-semiquinone state in substrate-reduced amine oxidases.	the role of copper in copper-containing amine oxidases has long been a source of debate and uncertainty. numerous electron paramagnetic resonance (epr) experiments, including rapid freeze-quench studies, have failed to detect changes in the copper oxidation state in the presence of substrate amines. one suggestion that copper reduction might occur, has never been confirmed. copper amine oxidases contain another cofactor, recently identified as 6-hydroxydopa quinone (topa quinone), which is reduc ...	1991	1846226
occurrence and ultrastructural characterization of bacteria in association with and isolated from azolla caroliniana.	the occurrence and ultrastructure of bacteria in leaf cavities of symbiotic azolla caroliniana were examined by transmission electron microscopy. bacteria were observed in all leaf cavities of azolla cultures. five ultrastructurally distinct types of bacteria were observed in each individual leaf cavity. features used to characterize the bacteria included morphology, cell wall structure, and cytoplasmic organization. at least one gram-positive and as many as four gram-negative types of bacteria ...	1991	1785935
direct evidence that ganglioside is an integral component of the thyrotropin receptor.	gangliosides were extracted from purified human and porcine thyrotropin (tsh) receptors (tsh-r) and were detected by probing with an 125i-labeled sialic acid-specific lectin, limax flavus agglutinin. gangliosides copurified with human and porcine tsh-r migrated between monosialoganglioside gm1 and disialoganglioside gd1a. ceramide glycanase digestion of the purified human tsh-r-associated glycolipid confirmed its ganglioside nature. it was resistant to vibrio cholerae sialidase, which digests al ...	1991	2000404
switching substrate preference of thermophilic xylose isomerase from d-xylose to d-glucose by redesigning the substrate binding pocket.	the substrate specificity of thermophilic xylose isomerase from clostridium thermosulfurogenes was examined by using predictions from the known crystal structure of the arthrobacter enzyme and site-directed mutagenesis of the thermophile xyla gene. the orientation of glucose as a substrate in the active site of the thermophilic enzyme was modeled to position the c-6 end of hexose toward his-101 in the substrate-binding pocket. the locations of met-87, thr-89, val-134, and glu-180, which contact ...	1991	2023950
l-phenylalanine production by double auxotrophic mutants of arthrobacter globiformis.	a number of tryptophan-plus-tyrosine double auxotrophs have been isolated from a glutamate producing arthrobacter globiformis excreting l-phenylalanine by two-step mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine. for the three potent mutants tested the medium of alföldi was found to be the best. the optimum tryptophan, tyrosine and biotin concentrations for phenylalanine production of these mutants were 0.5 mmol/l, 0.1 mmol/l and 5 micrograms/l, respectively. at these levels strain tt-39 y ...	1991	1841857
molecular cloning and nucleotide sequencing of the arthrobacter dextranase gene and its expression in escherichia coli and streptococcus sanguis.	a bacterial strain, which assimilated dextran and water-insoluble glucan produced by streptococcus mutans, was isolated from soil. the bacterium produced and secreted potent dextranase activity, which was identified as arthrobacter sp. and named cb-8. the dextranase was purified and some enzymatic properties were characterized. the enzyme efficiently decomposed the water-insoluble glucan as well as dextran. a gene library from the bacteria was constructed with escherichia coli, using plasmid puc ...	1991	1859672
marine biosurfactants, i. screening for biosurfactants among crude oil degrading marine microorganisms from the north sea.	three bacterial strains of marine origin were isolated during a screening for biosurfactants among n-alkane degrading microorganisms. one strain-identified as alcaligenes sp. mm1-produced a novel glucose lipid. in the case of arthrobacter sp. ek 1 the well-known trehalose tetraester was found as major component. from another pure culture classified as arthrobacter sp. si 1, extracellular emulsifying agents with properties indicating high molecular weight substances were detected. furthermore tre ...	1991	1878106
marine biosurfactants, ii. production and characterization of an anionic trehalose tetraester from the marine bacterium arthrobacter sp. ek 1.	within a screening for biosurfactants we could isolate various n-alkanes utilizing marine bacteria which were capable of synthesizing glycolipids. one strain was identified as arthrobacter sp. ek 1 which produced trehalose lipids. after purification by column and thick layer chromatography the main fraction, an anionic 2,3,4,2'-trehalose tetraester, was obtained. the chain lengths of fatty acids ranged from 8 up to 14, furthermore succinate could be detected. since the place of substitution of s ...	1991	1878107
[cloning of the arthrobacter globiformis fcba gene for dehalogenase and construction of a hybrid pathway of 4-chlorobenzoic acid degradation in pseudomonas putida].	the artrobacter globiformis kzt1 fcba gene responsible for dehalogenase (4-chlorobenzoate-4-hydroxylase) activity was cloned in escherichia coli and pseudomonas putida cells. the character of the fcba gene expression was studied. notwithstanding amplification of the gene dose and control of the inducible plac promoter, the level of substrate dehalogenation by recombinant e. coli strains was lower, as compared with that in the original kzt1 strain. cloning of the fcba gene in p. putida kz6r cells ...	1991	1879677
cloning and expression of the arthrobacter globiformis kzt1 fcba gene encoding dehalogenase (4-chlorobenzoate-4-hydroxylase) in escherichia coli.	the fsba gene controlling the first step of 4-chlorobenzoic acid (4cba) metabolism in the gram-positive soil bacterium arthrobacter globiformis kzt1 has been cloned and analysed in escherichia coli. the e. coli minicells analysis showed that a polypeptide(s) with mr = 58 kda (and/or mr = 32 kda) can be the fcba product(s). despite the gene dose amplification and control of the e. coli inducible plac promoter, the level of functional expression of the fcba gene in e. coli cells seems comparable o ...	1991	1884992
genetic control of degradation of chlorinated benzoic acids in arthrobacter globiformis, corynebacterium sepedonicum and pseudomonas cepacia strains.	the strains of arthrobacter globiformis kzt1, corynebacterium sepedonicum kz4 and pseudomonas cepacia kz2 capable of early dehalogenation and complete oxidation of 4-chloro-, 2,4-dichloro-and 2-chlorobenzoic acids, respectively, have been analyzed for the origin of the genetic control of degradation. the occurrence and molecular sizes of plasmids in all the strains have been established. plasmid pbs1501 was shown to control 4-chlorobenzoate dehalogenation in the case of kzt1 strain. the same pos ...	1991	1884993
experimental apparatus for selection of adherent microorganisms under stringent growth conditions.	a bioreactor apparatus is described for studying bacterial attachment. a cyclic, on-off, flow regime was imposed within the apparatus. model calculations illustrate the utility of this flow pattern in the selection and maintenance of slow-growing, adherent organisms. the apparatus is believed to have general utility in testing bacterial attachment influenced by many types of experimental or environmental constraints, including variations in fluid dynamics, presence of toxic substances (metals or ...	1991	1892388
the organic functional group in copper-containing amine oxidases. resonance raman spectra are consistent with the presence of topa quinone (6-hydroxydopa quinone) in the active site.	resonance raman spectroscopy has been used to probe the structure of the organic cofactor in copper-containing amine oxidases from bovine plasma, porcine kidney, pea seedlings, and the bacterium arthrobacter p1. the enzymes were first derivatized with phenylhydrazine or p-nitrophenylhydrazine; resonance raman spectra were obtained on the intact derivatized enzymes and on a derivatized active-site peptide isolated from bovine plasma amine oxidase. spectra of the intact amine oxidase phenylhydrazo ...	1991	1900285
mannopine and mannopinic acid as substrates for arthrobacter sp. strain mba209 and pseudomonas putida na513.	the characteristics of mannopine and mannopinic acid utilization by agrobacterium tumefaciens b6s3, arthrobacter sp. strain mba209, and pseudomonas putida na513 were studied. strain b6s3 utilized the four mannityl opines, mannopine, mannopinic acid, agropine, and agropinic acid. it also utilized several mannityl opine analogs, which were modified in either the sugar or the amino acid moiety. it utilized mannopine more rapidly after preincubation on mannopine, mannopinic acid, or glutamine than a ...	1991	1902209
structural determination of d-mannans of pathogenic yeasts candida stellatoidea type i strains: timm 0310 and atcc 11006 compared to ifo 1397.	the structures of the cell-wall d-mannans of pathogenic yeasts of candida stellatoidea type i strains, ifo 1397, timm 0310, and atcc 11006, were investigated by mild acid and, alkaline hydrolysis, by digestion with the arthrobacter gjm-1 strain exo-alpha-d-mannosidase, and by acetolysis. the modified d-mannans and their degradation products were studied by 1h- and 13c-n.m.r. analyses. d-manno-oligosaccharides released by acid treatment from the parent d-mannans were identified as the homologous ...	1991	1954627
continuous delta 1-hydrocortisone dehydrogenation with in situ product recovery.	a continuous aerated process for delta 1-hydrocortisone dehydrogenation by polyacrylamide-hydrazide (paah) bead-entrapped a. simplex cells was developed. the process allows for stable conversion of 1.6 g l-1 hydrocortisone (x 5 the solubility in water), made possible by the incorporation of selected cosolvent [5% (v/v) triethyleneglycol]. a large difference in substrate and product solubilities in the cosolvent-buffer medium allowed for in situ product recovery in an aerated, fluidized-bed, immo ...	1991	1367997
evidence for two distinct phosphonate-degrading enzymes (c-p lyases) in arthrobacter sp. glp-1.	arthrobacter sp. glp-1 can utilize a wide range of organophosphonates as its sole source of phosphorus. the in-situ formation of sarcosine and methane from glyphosate and methanephosphonic acid respectively was studied. these two processes are differentially induced during phosphorus-deprivation. methanephosphonic acid strongly inhibits glyphosate degradation (i50 10 microm), but glyphosate has very little effect on methane generation (i50 150 mm). the pattern of inhibition by other organophosph ...	1991	1368477
[quantitative description of microbial growth in a batch culture depending on the physiologic state of inocula].	the dynamics of biomass production and the respiration rate of five microorganisms grown as batch cultures were studied in detail. cell suspensions with a known physiological state, i.e. chemostat cultures grown at a particular d value, as well as quasi steady-state populations cultivated with slow feeding and long energy-source starvation were used as inocula. the microorganisms were arbitrary subdivided into two groups. the biomass of pseudomonas fluorescens, bacillus subtilis and debaryomyces ...	1991	1770867
immobilization of arthrobacter simplex in thermally reversible hydrogels: effect of gel hydrophobicity on steroid conversion.	arthrobacter simplex cells have been immobilized in a series of thermally reversible hydrogels having different gel hydrophobicities. steroid conversion from hydrocortisone to prednisolone via the delta 1-dehydrogenase system was greatly affected by the relative hydrophobicities of the gel matrices, which were prepared by copolymerizing varying ratios of n-isopropylacrylamide to acrylamide. the characteristics of the immobilized cells, such as optimal temperatures, km values, and the effects of ...	1991	1367990
stereospecific microbial reduction of 4,5-dihydro-4-(4-methoxyphenyl)-6-(trifluoromethyl-1h-1)-benzazepin+ ++-2-o ne.	a key intermediate, (3r-cis)-1,3,4,5-tetrahydro-3-hydroxy-4-(4-methoxyphenyl)-6-(trifluorome thyl)- 2h-1-benzazepin-2-one (compound ii or sq32191), with high optical purity was made by the stereoselective microbial reduction of the parent ketone 1. several strains of bacterial and yeast cultures were screened for the ability to catalyse the stereoselective reduction of 4,5-dihydro-4-(4-methoxyphenyl)-6-(trifluoromethyl)-1h-1-benzazepin++ +-2,3-dione [compound i or sq32425]. microorganisms from t ...	1991	1368001
antagonistic effect of coryneform bacteria from red smear cheese against listeria species.	a total of 187 coryneform bacteria were isolated from red smear and screened for inhibitory effects against 16 strains of listeria species. culture filtrates from brevibacterium linens (16 strains), arthrobacter nicotianae (4 strains) and arthrobacter nucleogenes (3 strains) showed clear zones of inhibition. the antagonistic effect was seen against 26 to 87% of 91 listeria strains tested. a. nicotianae and a. nucleogenes were more effective against listeria innocua and listeria ivanovii than aga ...	1991	1909545
choline oxidase, a catabolic enzyme in arthrobacter pascens, facilitates adaptation to osmotic stress in escherichia coli.	choline oxidase (ec 1.1.3.17) is a bifunctional enzyme that is capable of catalyzing glycine betaine biosynthesis from choline via betaine aldehyde. a gene (cox) encoding this enzyme in the gram-positive soil bacterium arthrobacter pascens was isolated and characterized. this gene is contained within a 1.9-kb fragment that encodes a polypeptide of approximately 66 kda. transfer of this gene to an escherichia coli mutant that is defective in betaine biosynthesis resulted in an osmotolerant phenot ...	1991	1987142
mechanism for aldose-ketose interconversion by d-xylose isomerase involving ring opening followed by a 1,2-hydride shift.	the active site and mechanism of d-xylose isomerase have been probed by determination of the crystal structures of the enzyme bound to various substrates, inhibitors and cations. ring-opening is an obligatory first step of the reaction and is believed to be the rate-determining step for the aldose to ketose conversion. the structure of a complex with a cyclic thio-glucose has been determined and it is concluded that this is an analogue of the michaelis complex. at -10 degrees c substrates in cry ...	1990	2319597
utilization of acrylonitrile by bacteria isolated from petrochemical waste waters.	a bacterium, utilising acrylonitrile as a sole source of carbon and nitrogen, was isolated from indian petrochemical corporation limited (ipcl) waste waters and identified as arthrobacter sp. this strain could also utilize acetonitrile, acetamide and acrylamide individually as a source of carbon and nitrogen. the metabolic studies with the whole cells indicated the sequential conversion of the nitrile to the respective amide and then to the respective acid and ammonia. the rate of nitrile hydrol ...	1990	2279769
observations of reaction intermediates and the mechanism of aldose-ketose interconversion by d-xylose isomerase.	crystallographic studies of d-xylose isomerase (d-xylose ketol-isomerase, ec 5.3.1.5) incubated to equilibrium with substrate/product mixtures of xylose and xylulose show electron density for a bound intermediate. the accumulation of this bound intermediate shows that the mechanism is a non-michaelis type. carrell et al. [carrell, h. l., glusker, j. p., burger, v., manfre, f., tritsch, d. & biellmann, j.-f. (1989) proc. natl. acad. sci. usa 86, 4440-4444] and the present authors studied crystals ...	1990	2304904
cell growth and enzyme synthesis of a mutant of arthrobacter sp. (dsm 3747) used for the production of l-amino acids from d,l-5-monosubstituted hydantoins.	a microorganism with the ability to form l-tryptophan from d,l-5-(3-indolyl-methyl)hydantoin (d,l-5-imh) was isolated and identified as arthrobacter sp. (dsm 3747). after isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high amounts of biomass with good specific activities concerning the enzymatic hydantoin-cleaving reactions. the ability of the microorganism to perform these bioconversions was found to b ...	1990	1366910
sites of cadmium uptake in bacteria used for biosorption.	electron microscopy and x-ray spectroscopy were used to determine location and type of cadmium biosorption on and in bacteria, some of which produced extracellular polymers. examined arthrobacter and pseudomonas species appear to have detoxification systems that precipitate cadmium internally irrespective of whether or not they excrete polymers. capsular klebsiella aerogenes strains showed minimal intracellular uptake but over a 5-100 mg dm-3 cd range produced the highest net metal removal level ...	1990	1366533
production of l-tryptophan from d,l-5-indolylmethylhydantoin by resting cells of a mutant of arthrobacter species (dsm 3747).	the reaction parameters and the stereospecificity of the enzymatic cleavage of d,l-5-indolylmethylhydantoin in producing l-tryptophan with resting cells of arthrobacter sp. dsm 3747 were studied. when intact cells were tested, the optimal ph was between 8.5 and 9.0 and the optimal temperature was 50 degrees c. both, l-n-carbamoylase and hydantoinase could be stabilized over 24 h at 30 and 40 degrees c by the addition of d,l-5-indolylmethylhydantoin. furthermore, the hydantoinase was stable over ...	1990	1366911
trimethyl lead degradation by free and immobilized cells of an arthrobacter sp. and by the wood decay fungus phaeolus schweinitzii.	the continuing production of leaded petrol generates liquid wastes containing recalcitrant trialkyl lead, for which no suitable chemical treatment has been formulated. this investigation explores the feasibility of using microorganisms to catalyse the rate-limiting step of trimethyl lead degradation to dialkyl lead; this disproportionates chemically to give, ultimately, pb2+ which is treatable by classical methods. an arthrobacter sp. and a wood decay macrofungus, phaeolus schweinitzii provide n ...	1990	1366364
the use of free and immobilised arthrobacter simplex in organic solvent/aqueous two-liquid-phase reactors.	the use of free and immobilised arthrobacter simplex (ncib 8929) for steroid delta 1-dehydrogenation in two-liquid-phase, stirred-tank reactors has been compared. product formation is related to the logarithm of the water-octanol partition coefficient (log p) of the organic solvent employed, but the relationship is different for the two forms of the biocatalyst. no reaction was seen with either biocatalyst in media containing solvents of log p less than or equal to 2.5. for free bacteria, produc ...	1990	1366455
nadh production from nad+ with a formate dehydrogenase system involving immobilized cells of a methylotrophic arthrobacter strain.	a convenient and economical method of nadh production from nad+ has been established using a formate dehydrogenase system involving immobilized cells of a methanol-utilizing bacterium. arthrobacter sp, km62. four kinds of cell entrapment were studied. an immobilized cell preparation showing a high nadh production activity was obtained by entrapment in a kappa-carrageenan gel lattice. the nadh-producing activity of the immobilized cells was investigated under various conditions. the nadh-producin ...	1990	1366576
purification and some properties of beta-fructofuranosidase i from arthrobacter sp. k-1.	arthrobacter sp. k-1, isolated from soil, produces beta-fructofuranosidase. this enzyme preparation was separated into three fractions (i, ii, and iii) by butyl-toyopearl 650 m column chromatography. the beta-fructofuranosidase i was purified to homogeneity by disc-electrophoresis after consecutive column chromatographies. the enzyme had a molecular weight of 52,000 by sds-polyacrylamide gel electrophoresis and 51,000 by gel filtration with ultrogel aca 44, and an isoelectric point of 4.3. the e ...	1990	1368550
methylamine oxidase from arthrobacter p1 as a prototype of eukaryotic plasma amine oxidase and diamine oxidase.	methylamine oxidase (maox) from gram-positive soil bacterium arthrobacter p1 catalyzes the oxidation of ch3nh2 to h2c = o and nh4+ via reduction of o2 to h2o2. past work indicates that maox is similar to mammalian plasma amine oxidase (pao) and diamine oxidase (dao), plant dao, and yeast peroxisomal amine oxidase (yao). all have mr congruent to 170,000 and are composed of 2 identical subunits, each of which contains 1 atom of cu(ii) and one molecule of quinonoid cofactor. herein, we report furth ...	1990	1965196
methylamine oxidase from arthrobacter p1.		1990	2280707
an examination of proton translocation and energy conservation during heterotrophic nitrification.	whether selected heterotrophic nitrifiers, as do the autotrophs, conserve energy during the oxidation of their nitrogenous substrates was studied. the examination of proton translocation of four different bacterial nitrifiers capable of pyruvic oxime [(po), ch3-c(noh)-cooh] nitrification and by an nh4+ oxidizing arthrobacter sp. was initiated. three of the po nitrifying bacteria, all pseudomonads, oxidize hydroxylamine (nh2oh) at a greater rate than po and yielded only stoichiometric protons whe ...	1990	2157622
growth of group a rotaviruses in a human liver cell line.	recent observations in children with rotavirus gastroenteritis and in infant mice given rotavirus vaccine by oral administration suggest that this well-known gastrointestinal pathogen may infect the liver. to examine this possibility, the susceptibility of hep g2 cells to infection with a variety of rotavirus strains was tested. these cells were used because they are considered to be well differentiated and exhibit many liver-specific functions. the hep g2 cells supported the growth of the simia ...	1990	2170264
catalytic mechanism of xylose (glucose) isomerase from clostridium thermosulfurogenes. characterization of the structural gene and function of active site histidine.	the gene coding for thermophilic xylose (glucose) isomerase of clostridium thermosulfurogenes was isolated and its complete nucleotide sequence was determined. the structural gene (xyla) for xylose isomerase encodes a polypeptide of 439 amino acids with an estimated molecular weight of 50,474. the deduced amino acid sequence of thermophilic c. thermosulfurogenes xylose isomerase displayed higher homology with those of thermolabile xylose isomerases from bacillus subtilis (70%) and escherichia co ...	1990	2229064
mechanism of enzymatic dehalogenation of pentachlorophenol by arthrobacter sp. strain atcc 33790.	pentachlorophenol (pcp) dehalogenase from arthrobacter sp. strain atcc 33790 converts pcp to tetrachlorohydroquinone. in labeling experiments with h(2)18o or 18o2, only with h(2)18o was labeled product found. however, unlabeled tetrachlorohydroquinone became labeled after incubation with the enzyme in h(2)18o. therefore, distinction between an oxygenolytic or a hydrolytic dehalogenation mechanism for the pcp dehalogenase is not possible.	1990	2254286
a survey of the microflora of raw and pasteurized milk and the sources of contamination in a milk processing plant in addis ababa, ethiopia.	the microorganisms present in raw and pasteurized milk and the sources of contamination in the milk after it had arrived at the processing plant in addis ababa were studied. the lowest count registered for raw milk samples was 4 x 10(7) cfu/ml while the highest was 1 x 10(9) cfu/ml. pasteurized milk had mesophilic aerobic counts of 7 x 10(5) cfu/ml as it left the pasteurizing unit, but the population increased 2- to 4-fold as a result of subsequent contamination. of the total counts in raw milk, ...	1990	2345191
rapid detection of extended ganglio-series gangliosides with terminal galnac beta 1-4gal sequence on high performance thin layer chromatography plates.	the mouse monoclonal antibody 2d4, which recognizes the terminal galnac beta 1-4gal-disaccharide of ggose3cer and ggose5cer, was used for the detection of ganglio-series gangliosides. the method involves separation of gangliosides on thin layer chromatography plates, followed by silica gel fixation, arthrobacter ureafaciens neuraminidase treatment and final immunostaining of desialylated gangliosides with the monoclonal antibody 2d4. both neuraminidase and the hybridoma 2d4 producing the specifi ...	1990	2350601
effect of organic amendments on bacterial multiplication in lake water.	in cayuga lake water amended with 30 micrograms of glucose or amino acids per ml, an added strain of pseudomonas fluorescens and indigenous bacteria grew extensively, pseudomonas sp. b4 and two rhizobia multiplied at a moderate extent, and introduced escherichia coli and klebsiella pneumoniae multiplied but to only a slight degree. the amendments did not enhance growth of micrococcus flavus and arthrobacter citreus, and an asporogenous strain of bacillus subtilis decreased in numbers. the pseudo ...	1990	2372214
characterization of phi ga1, an inducible phage particle from brevibacterium flavum.	eighteen related strains of arthrobacter, brevibacterium and corynebacterium were used as indicator strains in an attempt to isolate corynephages from a large number of soils and from waste-water samples. although no phages capable of producing plaques were isolated, one of the indicator strains used, brevibacterium flavum atcc 14067, was lysogenic for the inducible phage phi ga1. this phage was not observed to form plaques on any of the strains tested. phi ga1 is of b1-morphotype with a linear ...	1990	2391491
different types of dienelactone hydrolase in 4-fluorobenzoate-utilizing bacteria.	of various benzoate-utilizing bacteria tested, alcaligenes eutrophus 335, a. eutrophus h16, a. eutrophus jmp222, a. eutrophus jmp134, alcaligenes strain a7, and pseudomonas cepacia were able to grow with 4-fluorobenzoate as the sole source of carbon and energy. p. cepacia also utilizes 3-fluorobenzoate. except for a. eutrophus jmp134, which is known to grow with 2,4-dichlorophenoxyacetate and 3-chlorobenzoate (r. h. don and j. m. pemberton, j. bacteriol. 145:681-686, 1981), the strains were unab ...	1990	2394679
acii, a unique restriction endonuclease from arthrobacter citreus which recognizes 5' ccgc 3'.		1990	2170952
structural study of cell wall phosphomannan of candida albicans nih b-792 (serotype b) strain, with special reference to 1h and 13c nmr analyses of acid-labile oligomannosyl residues.	chemical structures of manno-oligosaccharides, from biose to heptaose, released from the phosphomannan of candida albicans nih b-792 strain (serotype b) by mild acid hydrolysis were investigated. the results of 1h nmr, 13c nmr, and fast atom bombardment mass spectrometry analyses confirmed that these manno-oligosaccharides belong to a homologous beta-1,2-linked series. although chemical shifts of 1h nmr patterns of these oligosaccharides were considerably too complicated to be assigned, their 13 ...	1990	2181936
soluble and cell-associated haemagglutinins of helicobacter (campylobacter) pylori.	some plate-grown strains of helicobacter (campylobacter) pylori that were harvested into phosphate-buffered saline and left for 1 h released soluble haemagglutinins. these caused high-titre agglutination of human and guinea-pig erythrocytes, whereas chicken, sheep and bovine erythrocytes were agglutinated at various titres. six of 10 strains which had been subcultured repeatedly did not possess soluble haemagglutinins. slide agglutination of bacterial suspensions demarcated the strains into two ...	1990	2258915
chlorinated biphenyl mineralization by individual populations and consortia of freshwater bacteria.	comparative studies were performed to investigate the contribution of microbial consortia, individual microbial populations, and specific plasmids to chlorinated biphenyl biodegradation among microbial communities from a polychlorinated biphenyl-contaminated freshwater environment. a bacterial consortium, designated lps10, was shown to mineralize 4-chlorobiphenyl (4cb) and dehalogenate 4,4'-dichlorobiphenyl. the lps10 consortium involved three isolates: pseudomonas testosteroni (lps10a), which m ...	1990	2117875