Publications

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kinetics of steroid-delta-1-dehydrogenase induction. 19744432774
a new species of arthrobacter which degrades picolinic acid. 19744598865
generic composition and nutritional requirements of bacteria isolated from three lakes. 19744599821
the metabolism and biological actions of coumarins. 19744617903
metabolism of 2-deoxy-d-glucose by baker's yeast. vi. a study on cell wall mannan. 19744601200
structure of the mannan from saccharomyces strain fh4c, a mutant constitutive for invertase biosynthesis. ii. protein moiety and components of the carbohydrate-peptide bonds. 19744603217
morphology and physiology of coryneform bacteria. 19744604791
the copolymeric structure of pig skin dermatan suplhate. characterization of d-glucuronic acid-containing oligosaccharides isolated after controlled degradation of oxydermatan sulphate.selective periodate oxidation of unsubstituted l-iduronic acid residues in copolymeric dermatan sulphate chains was followed by reduction-hydrolysis or alkaline elimination. by this procedure the glucuronic acid-containing periods were isolated in oligosaccharide form; general formula: [formula: see text] further degradation of these oligosaccharides with chondroitinase-ac yielded three types of products: (a) sulphated trisaccharide containing an unsaturated uronosyl moiety in the non-reducing t ...19744618474
peptidoglycans (mucopeptides): structure, function, and variations. 19744604888
relationships among coryneform bacteria from soil, cheese and sea fish. 19744605036
[the effect of organic substances on the assimilation of methane by obligate methylotrophs]. 19744857241
arthrobacter globiformis and its bacteriophage in soil.bacteriophages in soil for arthrobacter globiformis were rarely detected unless the soil was nutritionally amended and incubated. in amended soil, phage were continuously produced for at least 48 h, and this did not require the addition of host cells. rod and spheroid stage host cells added to the amended soil encountered indigenous bacteriophage, but added phage did not encounter sensitive indigenous host cells for some time, if at all. the indigenous phage in nonincubated soil seemed to be pre ...197416350007
ecology of soil arthrobacters in clarion-webster toposequences of iowa.toposequence variations in soil properties were characterized and related to variations in populations of total isolatable bacteria and arthrobacters. increases in soil no(3)-n, available phosphorous, no(3)-n-producing power, arthrobacter counts, and the percentage of the total counts represented by arthrobacters were correlated with decreases in soil acidity. the total bacterial counts were not correlated with soil acidity but were associated with percentage of soil organic matter and percentag ...197516350010
the effect of microbial mycolytic agents on trichophyton rubrum.chitinolytic microorganisms isolated from forest soil and from healthy gypsy moth larvae (porthetria dispar (l.) were screened for their ability to lyse trichophyton rubrum mycelia. a few of these isolates were mycolytic on both autoclaved and on actively growing, intact, t. rubrum mycelia. supernatants from these isolates, utilizing live t. rubrum as the sole carbon source, showed the same mycolytic ability. assays of the supernatants for enzymatic activity revealed exocellular, stable enzymes ...19751677
the microbial metabolism of acetophenone. metabolism of acetophenone and some chloroacetophenones by an arthrobacter species.1. an organism that utilizes acetophenone as sole source of carbon and energy was isolated in pure culture and tentatively identified as an arthrobacter sp. 2. cell-free extracts of the acetophenone-grown organism contained an enzyme, acetophenone oxygenase, that catalysed an nadph-dependent consumption of o(2) in the presence of the growth substrate; approx. 1mol of o(2) and 1mol of nadph were consumed per mol of acetophenone oxidized. 3. cell-free extracts also contained an enzyme capable of t ...19754061
survival of human enteric and other sewage microorganisms under simulated deep-sea conditions.the survival of pure cultures of escherichia coli, streptococcus faecalis, clostridium perfringens, and vibrio parahaemolyticus under simulated deep-sea conditions of low temperature (4 c), seawater, and hydrostatic pressures ranging from 1 to 1,000 atm was determined over a period exceeding 300 h. the viability of e. coli and total aerobic bacteria in seawater-diluted raw sewage subjected to these deep-sea conditions was also measured. there was a greater survival of both e. coli and s. faecali ...1975169733
bacteriostasis in soils sterilized by gamma irradiation and in reinoculated sterilized soils.an agar-disc method was used to compare the bacteriostatic properties of five soils with those of samples of the same soils sterilized by gamma irradiation. for three of the soils, bacteriostasis was removed either partially or entirely by sterilization. bacteriostasis was completely restored to sterile soil by reinoculating it with natural soil. all the reinoculation treatments restored the level of bacteriostasis equally effectively, whatever the origin of the soil used as inoculum.1975235358
purification and characterization of a polyphosphate kinase from arthrobacter atrocyaneus.polyphosphate kinase, an enzyme which incorporated the gamma-phosphate of atp into long-chain polyphosphate molecules, was purified more than 700-fold from arthrobacter atrocyaneus by ammonium sulphate fractionation, deae-cellulose column chromatography and ssphadex g-200 gel filtration. the enzyme had a broad ph optimum at 6-0 to 7-0 and required mn2+ or mg2+, histone, and inorganic phosphate for activity. the km for mn-atp was 0-53 mm, and for inorganic phosphate was 1-67 mm. free atp concentr ...1975168307
[evaluation of the cholinesterase activity of bacteria on a solid nutrient medium]. 1975241005
crystallization and some properties of chondroitinase from arthrobacter aurescens.a strain of arthrobacter aurescens which secretes a large amount of chondroitinase into a culture broth, was isolated from soil. the chondroitinase was purified 380-fold over culture broth in 24% yield and crystallized. some properties of the purified enzyme were studied and described: thermal stability (below 45 degrees), ph stability (ph 4.9 to 7.4), optimum temperature (50 degrees), and optimum ph (ph 6.0). chrondroitin sulfate a and c, chondroitin, and hyaluronic acid were split by the enzym ...1975234466
[distribution of pyrimidine blocks in the dna of brevibacterium linens, arthrobacter globiformis, nocardia corallina and nocardia rubra].the nucleotide composition and the frequency of pyrimidine blocks were studied in dna of the following bacteria: brevibacterium linens (weignamm, 1910) breed, 1953; arthrobacter globiformis (conn, 1928) conn et dimmick, 1947; nocardia corallina (bergey et al., 1923) waksman et henrici, 1948; nocardia rubra (krassilnikov, 1949) waksman et henrici, 1948. these organisms are classed by some microbiologists as mycobacteria (the mycobacteriaceae family) while other authors regard them as representati ...1975241002
transformation of morphine by resting cells and cell-free systems of arthrobacter sp.morphine can be transformed into 14-hydroxymorphine and a related unidentified material by resting cells of an arthrobacter species. cell-free extracts containing the transforming enzyme(s) have been obtained. o2, fe2+, and reduced nicotinamide adenine dinucleotide stimulate the transformation.1975240309
[isolation of bacteria assimilating ethanol from natural and industrial substrates]. 1975765694
a numerical taxonomic study of coryneform and related bacteria.two hundred and thirty-three strains of coryneform bacteria, including representatives of the genera arthrobacter, brevibacterium, cellulomonas, corynebacterium, erysipelothrix, jensenia, kurthia, listeria, microbacterium, mycobacterium, nocardia and propionibacterium and other related bacteria, were studied using 173 morphological, physiological and biochemical tests. the bacteria were grown on a soil extract medium which allowed growth of all the strains, and all were incubated at 30 degrees c ...1975805825
evidence for the occurrence of specific iron (iii)-binding compounds in near-shore marine ecosystems.aqueous extracts of samples from representtive types of near-shore marine ecosystems were examined for growth-promoting activity for the siderochrome auxotroph, arthrobacter jg-9. blue-green algal mat and sea grass samples gave strongly positive responses. relative to desferal (ciba pharmaceutical co., summit, n.j.), concentrations up to 110 ng/g (wet weight) of sample were found. in contrast, extracts of anaerobic muds or macroalgae gave no detectable activity. the hydroxamate type of iron chel ...1975809005
chondroitinase-producing bacteria in natural habitats.a search was undertaken for bacteria which degrade chondroitin sulfate in nature and to find bacteria with a usefully high rate of chondroitinase (chsase) productivity. first, 253 chsase-producing bacteria were obtained from aquatic and land environments in japan by aerobic and anaerobic screening methods. identification according to bergey's manual of determinative bacteriology or bain and shewan (1968) permitted assignment of the majority of the isolates to seven genera, aeromonas, vibrio, fla ...1975803822
covalently bound fad in d-6-hydroxynicotine oxidase. immunological studies of d- and l-6-hydroxynicotine oxidase: evidence for a d-enzyme precursor.antersera prepared against both enantiozymes, d- and l-6-hydroxynicotine oxidase, formed precipitins in double diffusion tests with their respective antigens only. a mixture of the two antisera caused spur formation of the two precipitin lines obtained with the pure enzymes. antiserum to l-apoprotein reacted with native l-enzyme and l-apoprotein but not with the d-sspecific enzyme. d-6-hydroxynicotine oxidase activity was inhibited by the anti-d-antiserum, leaving the l-enzyme fully active, whil ...1975809438
identification and flagellation of coryneform bacteria from poultry litter.thirth coryneform isolates from poultry litter were identified and checked for motility and flagellation. twenty-seven formed a yellow pigment and 3 were orange. twenty-three yellow strains were found to be arthrobacter citreus, although starch was hydrolyzed by these strains. four strains, including the three mainly pale yellow isolates, grew on citrate plus an ammonium salt and were classified as a. aurescens. three orange strains were found to be brevibacterium linens. only six strains of a. ...1975811345
the fine structure of the branched alpha-d-glucan from the blue-green alga anacystis nidulans: comparison with other bacterial glycogens and phytoglycogen.the fine structure of the glycogen from the blue-green alga anacystis nidulans has been examined. after selective hydrolysis of all (1 yields 6)-alpha-d linkages by a bacterial isoamylase, the resulting mixture of linear chains was subjected to gel permeation chromatography. for purposes of comparison, the glycogens from escherichia coli and arthrobacter sp., amylopectin, phytoglycogen from sweet corn, and shell-fish glycogen were treated similarly. the profiles of the unit chains of a nidulans ...1975806344
microflora and invert sugars in juice from healthy tissue of stored sugarbeets.bacterial populations increased in juice of healthy tissue of sugarbeet roots stored at 5 c. average counts showed a sixfold increase after 150 days of storage. invert sugar levels increased over threefold in "american 4 hybrid a" and remained fairly constant in "mono-hy d-2." the former cultivar also had significantly higher bacterial colony counts than the latter before 90 days of storage. of 36 isolates identified, 16 were pseudomonas spp. including p. chlororaphis; 6 bacillus spp. including ...1975808167
indicator media for microorganisms degrading chlorinated pesticides.a bromocresol purple liquid indicator medium and an eosin-methylene blue agar have been developed for the demonstration and isolation of microorganisms able to degrade the chlorinated herbicide, 2,4-dichlorophenoxyacetic acid (2,4-d). plates of the eosin-methylene blue agar indicate individual 2,4-d-degrading bacterial colonies. both indicator systems show the production of acid, presumably hydrochloric, during degradation of the 2,4-d in the media. concentrations of 2,4-d required to give an ac ...19751116035
[crystal-transformation of hydrocortisone effected by a culture of mycobacterium globiforme 193]. 19751117087
[formation of primary alcohols and palmitic acid in the microbiological oxidation of hexadecane].forty seven microbial strains oxidized hexadecane to form primary cetol and palmitic acid. the maximum quantitiy of the compounds was accumulated in the exponential phase of growing cultures (for 72 hours) and for 6 to 16 hours of incubation of resting cell suspensions. candida yeast were shown by the gas-liquid chromatography method to be the most active producers of cetol.19751187568
improvements of the membrane filter method for dna:rrna hybridization.we describe and recommend the following improvements of dna:rrna membrane filter hybridization methods. one of our aims was to avoid dna release from filter discs during hybridization. 1. our hybridization conditions are 2 ssc in aq. dest., with 20% formamide, 50 c, overnight for 16 hr. 2. duplexing is over in 8-10 hr. 3. formamide has to be very pure (o.d. less than or equal to 0.2/cm light path at 270 nm). 4. rnaase treatment: 250 mug/5 ml 2 ssc/filter at 37 c for 1 hr. 5. our conditions for s ...19751082296
a nutritional and taxonomic survey of arthrobacter soil isolates.one hundred and sixty arthrobacter soil isolates, four groups of 40 from each of four soil types, and 17 arthrobacter named strains were characterized on the basis of 203 different nutritional tests performed on each culture. one hundred and twenty-one compounds were examined as sole sources of carbon, 44 compounds as sole sources of nitrogen, 22 carbohydrates for the production of acid, and 16 tests were done for tolerance to various dyes. the four groups of soil arthrobacters differed markedly ...19751116046
arthrobacter luteus restriction endonuclease cleavage map of phi chi 174 rf dna. 19751081295
differentiation of arthrobacter soil isolates and named strains from other bacteria by reations on dye-containing media.twenty-five gram-negative and 62 gram-positive bacterial cultures, of which 37 werearthrobacters, were tested, using a multipoint inoculation device, for responses on varying concentrations of 35 dyes. both selective (growth vs. no growth) and differential (dye absorption) responses were obtained. it was possible to differentiate the arthrobacters from the other gram-positive bacteria tested as well as to distinguish separately each arthrobacter-named strain. the results indicated that dyes have ...19751125859
action of arthrobacter ureafaciens inulinase ii on several oligofructans and bacterial levans.1. arthrobacter ureafaciens inulinase ii which converts inulin to di-d-fructofuranose 1,2' : 2,3' dianhydride (difructose anhydride iii) leaving a small amount of oligosaccharides, was investigated in order to characterize its mode of action. 2. after the enzymatic reaction on the glucose-terminated inulin molecules had been completed, the oligosaccharides left in the enzyme digest were isolated, and identified to be the fructose-glucose oligosaccharides; o-beta-d-fructofuranosyl-(2 leads to 1)- ...19751148257
microbial metabolism of phenolic amines: degradation of dl-synephrine by an unidentified arthrobacter.microorganisms capable of degrading dl-synephrine were isolated from soil of citrus gardens by enrichment culture, with dl-synephrine as the sole source of carbon and nitrogen. an organism which appears to be an arthrobacter, but which cannot be identified with any of the presently recognized species was predominant in these isolates. it was found to metabolize synephrine by a pathway involving p-hydroxyphenylacetaldehyde, p-hydroxyphenylacetic acid, and 3,4-dihydroxyphenylacetic acid as interme ...19751150621
the microbial metabolism of di-n-butyl phthalate and related dialkyl phthalates. 19751125463
stereochemistry of delta 1,4 unsaturation in microbial transformation of cholesterol. 19751182921
induction of morphogenesis in the genus arthrobacter.thirty-eight different compounds were used with 17 species of arthrobacter to determine their ability to support growth and to induce the morphogenic cycle. in most cases, when a compound supported growth, it also induced the rod phase of growth. however, in a few cases, a compound would support growth with cells remaining in the coccoidal phase throughout the growth cycle. arthrobacter crystallopoietes was unique in the most compounds that supported growth did not induce the rod phase of the mo ...19751184572
[modifications of the antigenic composition of arthrobacter simplex atcc 6946 in the presence of reichstein's substance s]. 19751186519
microbial metabolism of 2-hydroxypyridine. 19751221039
peptidoglycan compositions of a new strain of arthrobacter crystallopietes during sphere-rod morphogenesis.arthrobacter crystallopoieties atcc 15481 was used to isolate a new strain. designated arthrobacter crystallopoieties epsr-16, which had a mass doubling time in brain heart infusion broth and in glucose/salts/yeast extract medium of 30 min compared to 2.40 h for the parent strain in similar media. the growth rates for the new strain and for the parent were close to 12 h in glucose/salts medium. the new strain formed well-separated cocci and diplococci in glucose/salts medium, and upon nutrient s ...19751201285
the microbial degradation of cyclohexane carboxylic acid. 19751202160
[microbiological transformation and degradation of pesticides]. 19751206144
oxidative degradation of squalene by arthrobacter species.an organism isolated from soil and identified as arthrobacter sp. was studied for its squalene degradation. the degradation product from squalene, which accumulated in the culture broth, was isolated and identified as trans-geranylacetone by mass spectrometry, gas chromatography, infrared spectrometry, and nuclear magnetic resonance spectrometry. addition of a high concentration of k2hpo4 to the culture medium resulted in accumulation of fairly large amounts of carboxylic acids in addition to ge ...19751115507
[facultative methylotroph belonging to the genus arthrobacter].a bacterial strain has been isolated and identified, on the basis of its morphological and physiologo-biochemical properties, as arthrobacter globiformis. the bacterium is a facultative methylotroph and grows not only on media with various organic compounds but also in the presence of methylated amines as a sole source of carbon, nitrogen, and energy. other c1-substrates were not utilized.19751214618
microbial decomposition of 2-ethylpyridine, 2,4-lutidine & 2,4,6-collidine. 19751218933
[synthesis of group b vitamins by bacteria of the genus arthrobacter]. 19751219320
pyrrolidine metabolism and its regulation in arthrobacter sp. 19751221030
sequential growth of bacteria on crude oil.by modification of the enrichment culture procedure three bacterial strains capable of degrading crude oil in sea water were isolated in pure culture, up-2, up-3, and up-4. strain up-2 appears to be highly specialized for growth on crude oil in sea water since it showed strong preference for oil or oil degradation products as substrates for growth, converted 66% of the oil into a form no longer extractable by organic solvents, quantitatively degraded the paraffinic fraction (gas chromatographic ...19751096820
microbiological characteristics of dungeness crab (cancer magister).aerobic, heterotropic microorganisms of dungeness crab (cancer magister) were isolated from raw crab, cooked crab, crab meats obtained during commercial processing, and from retail crab meat samples. each microbial isolate was then identified to the genus level employing the revised replica plating procedure. microbial groups most commonly isolated from crab meat were, in the order of predominance, moraxella, pseudomonas, acinetobacter, arthrobacter, micrococcus, flavobacterium-cytophaga, and ba ...19751096824
metabolism of dichlobenil by microorganisms in the aquatic environment. 19751150978
microbial cleavage of the tomatidine spiroketal sidechain. 19751177446
[arthrobacter siderocapsulatus isolated from lake water].two microbial strains have been isolated from lake water. the strains oxidize ferrous compounds and manganese. by the structure of microcolonies and the character of deposited oxides of these metals, the strains are identical to the genus siderocapsa. however, according to their growth cycle and some morpho-physiological characteristics, they were included into the genus arthrobacter (corynebacteriaceae). since these microorganisms differ, by their cultural and morpho-physiological properties, f ...19751177780
amino acid composition and physiochemical characterization of chondroitinase from arthrobacter aurescens.the amino acid and carbohydrate compositions of chondroitinase ac [ec 4.2.2.5] from arthrobacter aurescens were determined, and its physicochemical properties were examined. 1. the enzyme has been shown to be a glycoprotein containing mannose, glucose, glucosamine, and glucuronic acid (3:5:4:2). 2. its molecular wieght was estimated to be 76,000 by gel filtration on sephadex g-200, 75,000-80,000 by sds disc electrophoresis, and 75,800 by sedimentation veolcity. no subunits were detected in the m ...19751225917
[on the taxonomy and physiology of bacteria utilizing hydrocarbons in the sea (author's transl)].using mineral media with gas oil as sole carbon source, 191 bacterial strains were isolated from the costal area of marseille. these strains were attributed to achromobacter, alcaligenes, pseudomonas, acinetobacter and arthrobacter genera. amongst isolated strains there was a predominance of the alcaligenes-achromobacter group over others genera. growth and respiratory activity of 5 strains were studied on hexadecane and acetate. respiratory activity on hexadecane of 32 strains cultured on aceta ...19751190640
enzymic hydrolysis of di-d-fructofuranose 1, 2'; 2, 3' dianhydride with arthrobacter ureafaciens.enzymic hydrolysis of di-d-fructofuranose 1, 2'; 2, 3' dianhydride with the bacteria arthrobacter ureafaciens was studied to elucidate its mechanism. hydrolysis of the difructose dianhydride to d-fructose, which did not occur with yeast invertase [ec 3.2.1.26], was found to occur on incubation with an enzyme preparation from an autolysate of the above bacteria. however, incubation with enzyme which had been treated at 60 degrees for 30 min yielded an intermediate hydrolysis product. the product ...19751225919
bacterial carotenoids, xlvi. c50-carotenoids, 14. c50-carotenoids from arthrobacter glacialis.from the psychrophilic bacterium arthrobacter glacialis have been isolated three c50-carotenoids with molecular formulae c50h72o2: the bicyclic decaprenoxanthin (1a, 7% of total carotenoids), the aliphatic bisanhydrobacterioruberin (2a, 10%) and the monocyclic a.g. 470 (3a, 83%). decaprenoxanthin (1) and bisanhydrobacterioruberin (2) were in all respects, including chiroptical properties, identical with known carotenoids. the constitution of the previously undescribed a.g. 470 (3a) followed from ...19751211003
[spectrophotometric characteristics of the coccoid forms of actinomycetes].ir spectra of thw whole cells of the coccoid forms (mycococcus and micrococcus) isolated from lithophilous lichen were compared with ir spectra of the collection cultures of micrococcus and arthrobacter. generic spectral characteristics of mycococcus and micrococcus are presented. spectral heterogeneity within the genus arthrobacter complicates the diagnosis. the cultures of the mycococcus genus were divided into three groups according to their spectral characteristics. spectral scans of the stu ...19751214609
effects of temperature on the growth of psychrophilic bacteria from glaciers.growth of five strains of psychrophilic bacteria (four arthrobacter and one pseudomonas) isolated from glacial deposits was studied at different temperatures. three strains were facultative psychrophiles, having an optimum temperature for growth at about 25-28 degrees c and a maximum at about 32-34 degrees c. the two arthrobacter glacialis strains were found to be obligate psychrophiles with an optimum at 13-15 degrees c and a maximum at 18 degrees c. arrhenius plots showed that a. glacialis cou ...19761277005
colonization of soil by arthrobacter and pseudomonas under varying conditions of water and nutrient availability as studied by plate counts and transmission electron microscopy.arthrobacter globiformis and a pseudomonas soil isolate were incubated separately and in combination in soil that had been presterilized by autoclaving. growth and other responses of the cells in situ in this soil were monitored by plate counts and transmission electron microscopy examinations of cell sections. during the soil incubations, some of the samples were first allowed to dry and then were remoistened with water or with a dilute or a concentrated nutrient solution. based on plate counts ...19761267449
a specific endonuclease from arthrobacter luteus. 19761271462
action of chondroitinases. ii. numerical calculation of the degree of multiple attack.further investigation was carried out on the action patterns of two chondroitinase-ac [ec 4.2.2.5.] preparations obtained from arthrobacter aurescens and flavobacterium heparinum. to infer the action patterns of the chondroitinases, we proposed a new method for the calculation of the degree of multiple attack, based on the concept established by robyt and french ((1967) arch. biochem. biophys. 122, 8-16). it was shown that the degree of multiple attack (dm) is represented by the ratio of the ini ...19761018015
mechanism of reversible glycine cleavage reaction in arthrobacter globiformis. function of lipoic acid in the cleavage and synthesis of blycine. 19761259444
[effect of various carbon sources on cholinesterase formation by arthrobacter simplex var. cholinesterasus].the effect of carbohydrates, aromatic alcohols, choline and acetylcholine on the biomass production and biosynthesis of choline esterase was studied with arthrobacter simplex var. cholinesterasus. fructose was found to be the best carbon source for the biomass accumulation and synthesis of choline esterase. almost the same amount of the enzyme was produced on media with glucose and maltose as on the medium with fructose though the biomass yield was much lower. on the contrary, the biomass produc ...19761004262
[survival of epiphytic microorganisms following sublimation dehydration and storage].epiphytic microorganisms belonging to the genera pseudomonas, agrobacterium, achromobacter, flavobacterium, mycobacterium, mycococcus, nocardia, corynebacterium, arthrobacter, bacillus, and microbacterium were dehydrated by sublimation employing two suspension media, and were stored at 3-5 degrees c during 1-2.5 years. survival of epiphytic microorganisms upon dehydration by sublimation and storage was found to depend on their taxonomy (genus, species, strain) and the composition of the suspensi ...19761004279
identification of the cultivable bacteria in dental plaque from the beagle dog.fifteen different genera of microorganisms were isolated from the 27-month-old plaque of two beagle dogs. they were as follows: pseudomonas, proteus, neisseria, escherichia, staphylococcus, streptococcus, lactobacillus, nocardia, actinomyces, corynebacterium, micrococcus, microbacterium, brevibacterium, arthrobacter, and kurthia. the dog plaque was characterized by a low number of streptococci and the presence of several noncarbohydrate-fermenting organisms.19761069758
4-keto-gamma-carotene(beta, psi-caroten-4-one), the major pigment of an arthrobacter sp. 19761000359
the genetic map of bacteriophage phix174 constructed with restriction enzyme fragments. 19761084616
cleavage map of bacteriophage phix174 rf dna by restriction enzymes.phix rf dna was cleaved by restriction enzymes from haemophilus influenzae rf (hinf i) and haemophilus haemolyticus (hha. i). twenty one fragments of approximately 25 to 730 base pairs were produced by hinf i and seventeen fragments of approximately 40 to 1560 base pairs by hha i. the order of these fragments has been established by digestion on haemophilus awgyptius (hae iii) and arthrobacter luteus (alu i) endonuclease fragments of phix rf with hinf i and hha1. by this method of reciprocal dig ...19761085927
microbiological degradation of bile acids. the conjugation of a certain cholic acid metabolite with amino acids in corynebacterium equi.1. (4r)-4[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (ii) could not be utilized by arthrobacter simplex, even though the acid was one of the metabolites formed from cholic acid (i) by this organism. therefore the further degradation of the acid (ii) by corynebacterium equi was investigated to identify the intermediates involved in the cholic acid degradation. 2. the organism, cultured in a medium containing the acid (ii) as the sole source of carbon, ...19761016254
degradation of alpha-linked d-gluco-oligosaccharides and dextrans by an isomalto-dextranase preparation from arthrobacter globiformis t6. 1976938503
microbiological degradation of bile acids. the preparation of some hypothetical metabolites involved in cholic acid degradation.1. to identify the intermediates involved in the degradation of cholic acid, the further degradation of (4r)-4-[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (iva) by arthrobacter simplex was attempted. the organism could not utilize this acid but some hypothetical intermediate metabolities of compound (iva) were prepared for later use as reference compounds. 2. the nor homologue (iiia) and the dinor homologue (iiib) of compound (iva) were prepared by e ...1976942388
[preliminary observations on the characterization of a microbial strain isolated from mud and active in saline medium with added phenanthrene]. 19761016622
nalpha-acetylfusarinines: isolation, characterization, and properties.the isolation, purification, and structural elucidation of a new group of naturally occurring hydroxamic acids are described. these compounds are produced by an unidentified species of penicillium when grown on iron deficient medium. they are closely related structurally to the fusarinines, whose basic structure contains ndelta-(cis-5-hydroxy-3-methylpent-2-enoyl)-ndelta-hydroxy-l-ornithine, but the alpha-amino groups in the new compounds are protected by nalpha acetyl groups. the most complex o ...1976949471
on the regulation of histidinol dehydrogenase induction in arthrobacter histidinolovorans. 1976971316
conversion of (+/-)-synephrine into p-hydroxyphenylacetaldehyde by arthrobacter synephrinum. a novel enzymic reaction.a partically purified enzyme from arthrobacter synephrinum was found to catalyse the conversion of (+/-)-synphrine into p-hydroxyphrenylacetaldehyde and methylamine. the enzyme is highly specific for synephrine and is distinctly different from monoamine oxidase.19761008837
[degradation of 4-chlorobenzoic acid by an arthrobacter species (author's transl)].an arthrobacter sp. growing on 4-chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. protocatechuic acid is further attacked by "meta"-cleavage. during growth of the arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from the medium, suggesting the involvement of the "ortho"-cleavage pathway. the enzymes both for the "meta"- and the "ortho"-cleavage pathway are inducible.19761015950
ring hydroxylation of p-chlorophenylacetate by an arthrobacter strain.resting cell suspensions of a strain of arthrobacter grown on phenylacetate converted p-chlorophenylacetate to two products. one of the products was identified as 4-chloro-3-hydroxyphenylacetate.1976970937
induction of d-6-hydroxynicotine oxidase in resting cells of arthrobacter oxidans.resting cell suspensions of arthrobacter oxidans were shown to synthesize the inducible enantiozyme, d-6-hydroxynicotine oxidase, in the presence of d-nicotine or d-6-hydroxynicotine. the corresponding l-enantiomers, as well as gamma-methylaminopropyl-(6-oh-pyridyl-3)-ketone, which is the product of the reaction catalyzed by the enzyme, were ineffective as inducers. l-6-hydroxynicotine inhibited induction by d-nicotine and d-6-hydroxynicotine while l-nicotine inhibited induction by d-6-hydroxyni ...19761015955
macromolecular synthesis and cell division during morphogenesis of arthrobacter crystallopoietes.the sphere-rod-sphere morphology cycle of arthrobacter crystallopoietes was accompanied by changes in the rate of growth and the rates of dna, rna and protein synthesis. the patterns of macromolecule synthesis resembled those found in other bacteria during a step-up followed by a step-down in growth rate. during the step-up in growth spherical cells grew into rods and macromolecules were synthesized in the absence of cell division. during step-down, successive rounds of septation produced progre ...19761015960
[mode of action of d-amino acids on the biosynthesis of peptidoglycan (author's transl)].the mechanism of growth inhibition by d-amino acids was studied. d-serine at concentrations from 0.02-0.2 m was sufficient to cause partial growth inhibition in seven species of bacteria representing the four most common types of peptidoglycan. the inhibited cells displayed morphological alterations. in the nucleotide-activated peptidoglycan precursors of these cells, d-alanine residues in position 4 and/or 5 of the peptide moiety were partially or even completely replaced by d-serine. the pepti ...1976825075
adjuvant activity of bacterial glycolipids. 1976825676
microbiological characteristics of natural mineral water.natural, non-carbonated mineral water is, like every other natural water from a spring, never sterile. however, the microbial level is always very low. but after its bottling, the level rises rapidly and numbers of more than 10,000 to 100,000/ml can be reached. in principle 2 groups of bacteria of very different origin and properties can be found in the microbial flora of the bottled, non-carbonated mineral water. allochthonous bacteria will get into the water by contamination from the container ...1976829205
growth and pigment production by arthrobacter pyridinolis n. sp.a new bacterium capable of growing on 2-hydroxypyridine as sole source of carbon and nitrogen was isolated from soil. during its growth on solid medium, approximately 50% of this substrate was converted to a brilliant blue crystalline pigment which was deposited extracellularly in the colony mass. the pigment was identical to that produced by arthrobacter crystallopoietes during its growth on 2-hydroxypyridine. the new isolate exhibited the typical cycle of morphogenesis characteristic of the ge ...19761015948
natural paucity of anaplerotic enzymes: basis for dependence of arthrobacter pyridinolis on l-malate for growth.previous work has shown that in arthrobacter pyridinolis the transport systems for glucose and several amino acids are respiration coupled, with malate oxidation occurring concomitantly with transport. the requisite malate has to be supplied exogenously, so that growth on glucose or certain amino acids only occurs if malate is also present in the medium. these and other data suggested that a. pyridinolis might be deficient in anaplerotic enzymes, which maintain intracellular levels of dicarboxyl ...1976931946
[oxidation and assimilation pathways of methylated amines in arthrobacter globiformis].the oxidation by arthrobacter globiformis b-175 of tertiary, secondary, and primary amines is catalyzed by specific induced enzymes. trimethylamine is oxidized to dimethylamine via trimethylamine n-oxide by monooxygenase and demethylase. dimethylamine is converted to methylamine by another monooxygenase. methylamine is oxidized to ammonia and formaldehyde by aminoxidase. formaldehyde is assimilated via ribulose monophosphate cycle, which is confirmed by the presence of hexose-phosphate synthase ...1976933867
soybean flower-to-seed movement of epiphytic bacteria.epiphytic tracer bacteris (a pseudomonas sp. and an arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. seed from two of these pods also carried tracers.1976943220
[current achievements in the study of the structure and specificity of flavin enzymes]. 1976944972
motility as a morphogenic character in the genus arthrobacter.motility in arthrobacter atrocyaneus, a. citreus, and a. simplex was found to correlate with the morphogenic cycle of these organisms. the percentage of the a. atrocyaneus and a. simplex populations that were flagellated at a given time during the growth cycle differed significantly from that of the normorphogenic pseudomonas aeruginosa population. flagellation in a. atrocyaneus was shown to be dependent upon the morphogenic cycle rather than upon growth. the commitment to flagellar synthesis in ...1976956128
thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa.acid methanolysates of strains representing 58 coryneform taxa were examined for mycolic acids and other long-chain constituents by thin-layer chromatography. mycolic esters were detected in the methanolysates of true corynebacteria but not in those from plant pathogenic bacteria, corynebacterium haemolyticum, corynebacterium pyogenes or from representatives of the genera arthrobacter, cellulomonas, curtobacterium, kurthia or oerskovia. thin-layer chromatography of whole-organism methanolysates ...1976825611
[effect of nitrogen source on growth of arthrobacter simplex and its biosynthesis of cholinesterase].the effect of organic and inorganic forms of nitrogen on biomass accumulation and cholinesterase synthesis was studied with arthrobacter simplex var. cholinesterasus. the culture assimilates nitrogen of ammonium compounds better than other forms of inorganic nitrogen; the best nitrogen source for biosynthesis of cholinesterase is ammonium phosphate. nitrogen of nitrates is not assimilated. the amount of biomass is almost twice as high on the medium with peptone, casein or casein hydrolysate as o ...1976979679
the nucleotide sequence of a dna fragment, 71 base pairs in length, near the origin of dna replication of bacteriophage 0x174.part of the nucleotide sequence of a restriction fragment covering the origin of phix174 dna replication 1 has been determined. the fragment a7c was obtained by digestion of phix174 rf dna by the restriction enzyme from arthrobacter luteus, alu 1. it was further cleaved into two fragments, one large and one small, by the action of the restriction enzyme from haemophilus aegyptius, hae 111. the nucleotide sequence of the small fragment has been determined by analysis of the transcription products ...1976995652
[degradation of (-)-ephedrine by arthrobacter globiformis (author's transl)].a bacterium which grows on and completely degrades ephedrine as the only source of carbon was isolated from soil. this bacterium was identified as arthrobacter globiformis. from the culture medium the following metabolites were isolated: 1-hydroxy-1-phenyl acetone, benzoic acid, pyrocatechol, cis-cis-muconic acid and methylamine. the mechanism of metabolism is discussed and compared with that in mammals.1976998048
facultative wood-digesting bacteria from the hind-gut of the termite reticulitermes hesperus.among the facultative bacteria capable of growth on mesquite wood which were isolated from the asceptically dissected hind-gut of the termite reticulitermes hesperus were two strains of bacillus cereus, one strain each of arthrobacter, alcaligenes and serratia, and a very small gram-negative fermentative rod. the b. cereus strains, the serratia marcescens strain and the arthrobacter sp. grew well on a mineral salts alpha-cellusose agar. one of the bacillus cereus strains and serratia marcescens ...1976822127
restriction-enzyme-cleavage maps of bacteriophage m13. existence of an intergenic region on the m13 genome.replicative form dna of bacteriophage m13 was cleaved into specific fragments by an endonuclease isolated from hemophilus aegyptius (endor.haeii) and an endonuclease from arthrobacter luteus (endor.alui). the fragments were ordered as to construct a circular map of the phage m13 genome by: (a) using each fragment as a primer for the synthesis in vitro of its respective neighbour and (b) digesting the isolated fragments with the hemophilus aegyptius enzyme endor.haeii or the hemophilus aphirophil ...1976786638
observations on lysogeny in glutamic acid bacteria.induced lysis occurred in a number of different strains of glutamic acid bacteria. mixed culture experiments indicated that induced cultures produce phages or bacteriocins. temperate phages were isolated from two related strains of brevibacterium divaricatum.1976823863
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