Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| bacterial communities of tyre monofill sites: growth on tyre shreds and leachate. | to investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria. | 2009 | 19239530 |
| actinobacterial community dynamics in long term managed grasslands. | palace leas, a long-term experiment at cockle park farm, northumberland, uk was established in winter 1896-1897 since when the 13 plots have received regular and virtually unchanged mineral fertiliser and farm yard manure inputs. fertilisers have had a profound impact on soil ph with the organically fertilised plots showing a significantly higher ph than those receiving mineral fertiliser where ammonium sulphate has led to soil acidification. here, we investigate the impact of organic and minera ... | 2009 | 19247797 |
| structural basis and catalytic mechanism for the dual functional endo-beta-n-acetylglucosaminidase a. | endo-beta-n-acetylglucosaminidases (engases) are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation reactions. recently, these enzymes have become the focus of intense research because of their potential for synthesis of glycopeptides. we have determined the 3d structures of an engase from arthrobacter protophormiae (endo-a) in 3 forms, one in native form, one in complex with man(3)glcnac-thiazoline and another in complex with glcnac-asn. the carbohydrate moie ... | 2009 | 19252736 |
| a polyphasic study on the species diversity of the sediment microbiota of lake hévíz. | lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ... | 2009 | 20038486 |
| genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils. | three parathion-degrading bacteria and eight pairs of bacteria showing syntrophic metabolism of parathion were isolated from rice field soils, and their genetic and phenotypic characteristics were investigated. the three isolates and eight syntrophic pairs were able to utilize parathion as a sole source of carbon and energy, producing p-nitrophenol as the intermediate metabolite during the complete degradation of parathion. analysis of 16s rrna gene sequence indicated that the isolates were rela ... | 2009 | 20075637 |
| heterologous expression and characterization of choline oxidase from the soil bacterium arthrobacter nicotianae. | in the course of a microbial screening of soil samples for new oxidases, different enrichment strategies were carried out. with choline as the only carbon source, a microorganism was isolated and identified as arthrobacter nicotianae. from this strain, a gene coding for a choline oxidase was isolated from chromosomal dna. this gene named coda was cloned in escherichia coli bl21-gold and the protein (an_coda) heterologously overexpressed as a soluble intracellular protein of 59.1 kda. basic bioch ... | 2009 | 18787818 |
| structural characterization and antithrombin activity of dermatan sulfate purified from marine clam scapharca inaequivalvis. | glycosaminoglycans from the body of marine clam scapharca inaequivalvis were extracted at about 0.15- 0.18 mg/g of dry tissue, composed of dermatan sulfate (ds) (approx. 74%) and heparan sulfate (26%). after treatment with nitrous acid, ds was isolated for further complete structural characterization. agarose-gel electrophoresis in combination with various enzymes, chondroitin abc lyase, chondroitin b lyase, chondroitin acii lyase from arthrobacter aurescens, and chondroitin ac lyase from flavob ... | 2009 | 19056786 |
| the oligomeric assembly of the novel haem-degrading protein hbps is essential for interaction with its cognate two-component sensor kinase. | hbps, a novel protein of previously unknown function from streptomyces reticuli, is up-regulated in response to haemin- and peroxide-based oxidative stress and interacts with the sens/senr two-component signal transduction system. in this study, we report the high-resolution crystal structures (2.2 and 1.6 a) of octomeric hbps crystallized in the presence and in the absence of haem and demonstrate that iron binds to surface-exposed lysine residues of an octomeric assembly. based on an analysis o ... | 2009 | 19244623 |
| preliminary study of a microbeads based histamine detection for food analysis using thermostable recombinant histamine oxidase from arthrobacter crystallopoietes kait-b-007. | we designed and prepared a micro biosensing system consisting of a flow through system with a sub-micro liter injection valve and a sub-micro liter volume bioreactor. an electrochemical detector was combined with the reactor for immediate detections. the volumes of the reactor and the sample loop for the injection were 850 nl and 320 nl, respectively. this paper described about the characteristics of the sensing system in the case of histamine detection for food analysis. histamine oxidase from ... | 2009 | 19064110 |
| decomposition of cr(v)-diols to cr(iii) complexes by arthrobacter oxydans. | we demonstrated previously that cr(vi) is readily reduced to oxocr(v)-diols at the surface of arthrobacter oxydans-a gram-positive aerobic bacteria isolated from columbia basalt rocks originated from a highly contaminated site in the usa. here, we report an electron spin resonance (esr) study of cr(iii) hydroxide formation from cr(v)-diols by this bacterial strain as cells were exposed to 35, 200, and 400 mg/l of cr(vi) under aerobic conditions as a batch culture and as lyophilized cells. the ti ... | 2009 | 19067030 |
| arthrobacter sp. lipase immobilization for preparation of enantiopure masked beta-amino alcohols. | recent reports on immobilization of lipase from arthrobacter sp. (abl, mtcc 5125; iiim isolate) on insoluble polymers have shown altered properties including stability and enantioselectivity. present work demonstrates a facile method for the preparation of enantiopure beta-amino alcohols by modulation of abl enzyme properties via immobilization on insoluble as well as soluble supports using entrapment/covalent binding techniques. efficacies of immobilized abl on insoluble supports prepared from ... | 2009 | 19081255 |
| isolation and characterization of mesotrione-degrading bacillus sp. from soil. | dissipation kinetics of mesotrione, a new triketone herbicide, sprayed on soil from limagne (puy-de-dôme, france) showed that the soil microflora were able to biotransform it. bacteria from this soil were cultured in mineral salt solution supplemented with mesotrione as sole source of carbon for the isolation of mesotrione-degrading bacteria. the bacterial community structure of the enrichment cultures was analyzed by temporal temperature gradient gel electrophoresis (ttge). the ttge fingerprint ... | 2009 | 19121884 |
| genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils. | arsenic is known as a toxic metalloid, which primarily exists in inorganic form [as(iii) and as(v)] and can be transformed by microbial redox processes in the natural environment. as(iii) is much more toxic and mobile than as(v), hence microbial arsenic redox transformation has a major impact on arsenic toxicity and mobility which can greatly influence the human health. our main purpose was to investigate the distribution and diversity of microbial arsenite-resistant species in three different a ... | 2009 | 19128515 |
| crystallographic, spectroscopic, and computational analysis of a flavin c4a-oxygen adduct in choline oxidase. | flavin c4a-oo(h) and c4a-oh adducts are critical intermediates proposed in many flavoenzyme reaction mechanisms, but they are rarely detected even by rapid transient kinetics methods. we observe a trapped flavin c4a-oh or c4a-oo(h) adduct by single-crystal spectroscopic methods and in the 1.86 a resolution x-ray crystal structure of choline oxidase. the microspectrophotometry results show that the adduct forms rapidly in situ at 100 k upon exposure to x-rays. density functional theory calculatio ... | 2009 | 19133805 |
| plasmid as a measure of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid. | the purpose of this research was to pursuit the quantification of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid (2,4-d) by detecting and quantifying a prominent 2,4-d degradation encoding plasmid. batch reactor acclimation, de-acclimation, and re-acclimation tests were conducted during which periods the courses of 2,4-d dissipation and plasmid evolution were quantitatively measured. pure cultures of bacterial strains were detected to give rise to a plasmid approximately the s ... | 2009 | 18930390 |
| liquid chromatography-mass spectrometry to study chondroitin lyase action pattern. | liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. two commercial enzymes, chondroitinase abc (proteus vulgaris) and chondroitinase acii (arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. next, the action patterns of recombinant lyases, chondroitinase abc from bacteroides thetaiotaomicron (expressed in escherichia coli) and chondroitinase ac from flav ... | 2009 | 18992215 |
| endophytic bacterial flora in root and stem tissues of black pepper (piper nigrum l.) genotype: isolation, identification and evaluation against phytophthora capsici. | to isolate and identify black pepper (piper nigrum l) associated endophytic bacteria antagonistic to phytophthora capsici causing foot rot disease. | 2009 | 19018963 |
| high rate of n2 fixation by east siberian cryophilic soil bacteria as determined by measuring acetylene reduction in nitrogen-poor medium solidified with gellan gum. | for evaluating n(2) fixation of diazotrophic bacteria, nitrogen-poor liquid media supplemented with at least 0.5% sugar and 0.2% agar are widely used for acetylene reduction assays. in such a soft gel medium, however, many n(2)-fixing soil bacteria generally show only trace acetylene reduction activity. here, we report that use of a n(2) fixation medium solidified with gellan gum instead of agar promoted growth of some gellan-preferring soil bacteria. in a soft gel medium solidified with 0.3% ge ... | 2009 | 19286791 |
| construction, expression and characterization of fusion enzyme from arthrobacter oxydans dextranase and klebsiella pneumoniae amylase. | an artificial fusion protein of arthrobacter oxydans dextranase and klebsiella pneumoniae alpha-amylase was constructed and expressed in escherichia coli. most of the expressed protein existed as an insoluble fraction, which was solubilized with urea. the purified fusion enzyme electrophoretically migrated as a single protein band; m = 137 kda, and exhibited activities of both dextranase (10.8 u mg(-1)) and amylase (7.1 u mg(-1)), which were lower than that of reference dextranase (13.3 u mg(-1) ... | 2009 | 19305953 |
| the x-ray crystal structure of an arthrobacter protophormiae endo-beta-n-acetylglucosaminidase reveals a (beta/alpha)(8) catalytic domain, two ancillary domains and active site residues key for transglycosylation activity. | glycoside hydrolase family gh85 is a family of endo-beta-n-acetylglucosaminidases that is responsible for the hydrolysis of beta-1,4 linkage in the n,n-diacetylchitobiose core of n-linked glycans. the endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) is of particular interest, given its increasing use for the chemoenzymatic synthesis of bespoke n-glycans using n-glycan oxazolines as glycosyl donors. the e173q variant of endo-a is especially attractive for synthesis, as i ... | 2009 | 19327363 |
| four novel arthrobacter species isolated from filtration substrate. | four gram-positive, non-motile, non-spore-forming bacterial strains, lc4(t), lc6(t), lc10(t) and lc13(t), were isolated from a filtration substrate made from trass, a volcanic rock, and their taxonomic positions were investigated by a polyphasic taxonomic approach. the novel strains grew over a temperature range of 5-40 degrees c, at ph values of 6-11 and in the presence of 3-7 % (w/v) nacl. a phylogenetic tree based on 16s rrna gene sequences showed the novel strains formed a distinct evolution ... | 2009 | 19329620 |
| arthrobacter halodurans sp. nov., a new halotolerant bacterium isolated from sea water. | a novel gram-positive, halotolerant, non-sporulating, non-motile, catalase-positive, oxidase-negative and aerobic bacterium, designated strain jsm 078085(t), was isolated from sea water collected from the south china sea. strain jsm 078085(t) exhibited a rod-coccus growth cycle and produced a yellow pigment. the strain was able to grow in the presence of 0-12% (w/v) nacl and at ph 6.0-9.5 and 4-35 degrees c; optimum growth was observed at ph 7.0 and 25-30 degrees c in the absence of nacl. the pe ... | 2009 | 19337850 |
| enzymatic glycosylation of triazole-linked glcnac/glc-peptides: synthesis, stability and anti-hiv activity of triazole-linked hiv-1 gp41 glycopeptide c34 analogues. | long-lasting sweet proteins: the chemoenzymatic synthesis of a triazole (t)-linked glycosylated c34 fragment from hiv-1 gp41 is described. the glycopeptide shows high solubility, excellent fusion inhibition, and as shown in the graph, promising protease resistance. endoglycosidase-catalyzed transglycosylation of triazole-linked glucose (glc) and n-acetylglucosamine (glcnac)-containing dipeptides and polypeptides was achieved by using synthetic sugar oxazoline as the donor substrate. it was found ... | 2009 | 19353609 |
| a novel nicotine catabolic plasmid pmh1 in pseudomonas sp. strain hf-1. | attempts were made to acquire a plasmid-loss mutant via various methods (spontaneous mutation, sds, and mitomycin c), among which the method involving mitomycin c (10 microg/ml) has been proven successful. concomitant with the loss of the plasmid in pseudomonas sp. strain hf-1, the cured derivative was identified as having a nicotine-negative (nic-) phenotype, named mutant strain 6-13 (nic-). after plasmids were transferred from strain hf-1 (named plasmid pmh1) to the mutant strain 6-13, the mut ... | 2009 | 19370065 |
| effect of immobilization of a bacterial consortium on diuron dissipation and community dynamics. | this work intended to study the relationship between diuron herbicide dissipation and the population dynamics of co-cultivated delftia acidovorans wdl34 (wdl34) and arthrobacter sp. n4 (n4) for different cell formulations: free cells or immobilization in ca-alginate beads of one or both strains. gfp-tagged wdl34 and n4 gram staining allowed analyzing the cell growth and distribution of each strain in both beads and culture medium in the course of the time. compared to the free cell co-culture of ... | 2009 | 19386487 |
| contribution of flavin covalent linkage with histidine 99 to the reaction catalyzed by choline oxidase. | the fad-dependent choline oxidase has a flavin cofactor covalently attached to the protein via histidine 99 through an 8alpha-n(3)-histidyl linkage. the enzyme catalyzes the four-electron oxidation of choline to glycine betaine, forming betaine aldehyde as an enzyme-bound intermediate. the variant form of choline oxidase in which the histidine residue has been replaced with asparagine was used to investigate the contribution of the 8alpha-n(3)-histidyl linkage of fad to the protein toward the re ... | 2009 | 19398559 |
| biodegradation of phenanthrene and pyrene in compost-amended soil. | this study investigated the biodegradation of the polycyclic aromatic hydrocarbons (pahs) phenanthrene and pyrene in compost and compost-amended soil. the degradation rates of the two pahs were phenanthrene>pyrene. the degradation of pah was enhanced when the two pahs were present simultaneously in the soil. the addition of either of the two types of compost (straw and animal manure) individually enhanced pah degradation. compost samples were separated into fractions with various particle size r ... | 2009 | 19412845 |
| high yield synthesis of 12-aminolauric acid by "enzymatic transcrystallization" of omega-laurolactam using omega-laurolactam hydrolase from acidovorax sp. t31. | the genes encoding omega-laurolactam hydrolases from cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, and sphingomonas sp. u238 were cloned and sequenced. nucleotide and amino acid sequence analysis of the four genes indicated that the primary structures of these omega-laurolactam hydrolases are significantly similar to the 6-aminohexanoate-cyclic-dimer hydrolase (ec 3.5.2.12). these genes were expressed in escherichia coli, and the omega-laurolactam hydrolysing activity of the reco ... | 2009 | 19420719 |
| effects of proteus vulgaris growth on the establishment of a cheese microbial community and on the production of volatile aroma compounds in a model cheese. | to investigate the impact of proteus vulgaris growth on a multispecies ecosystem and on volatile aroma compound production during cheese ripening. | 2009 | 19426267 |
| improvement of catalytic properties of lipase from arthrobacter sp. by encapsulation in hydrophobic sol-gel materials. | in this work, lipase from arthrobacter sp. was immobilized by sol-gel encapsulation to improve its catalytic properties. various silanizing agents including vinyl-trimethoxy silane, octyl-trimethoxy silane, gamma-(methacryloxypropyl)-trimethoxy silane (maptms) and tetraethoxysilane (teos) were chosen as the precursors. among them, maptms was for the first time utilized to encapsulate lipases, and the prepared enzyme by copolymerization of maptms and teos exhibited the highest activity in both th ... | 2009 | 19428242 |
| ecotoxicological characterisation of 12 incineration ashes using 6 laboratory tests. | in the european waste list (2000/532/ec as amended) the ash of municipal waste incineration is defined as a so called mirror entry. this waste can be classified as hazardous or non-hazardous depending on the content of hazardous substances and other risk properties. for the assignment of waste in mirror entries, 14 criteria are defined. one of the criteria is h14 "ecotoxic". in the presented study, the ecotoxicological potential of 12 ashes from different incineration plants has been assessed us ... | 2009 | 19442505 |
| uptake of l-nicotine and of 6-hydroxy-l-nicotine by arthrobacter nicotinovorans and by escherichia coli is mediated by facilitated diffusion and not by passive diffusion or active transport. | the mechanism by which l-nicotine is taken up by bacteria that are able to grow on it is unknown. nicotine degradation by arthrobacter nicotinovorans, a gram-positive soil bacterium, is linked to the presence of the catabolic megaplasmid pao1. l-[(14)c]nicotine uptake assays with a. nicotinovorans showed transport of nicotine across the cell membrane to be energy-independent and saturable with a k(m) of 6.2+/-0.1 microm and a v(max) of 0.70+/-0.08 micromol min(-1) (mg protein)(-1). this is in ac ... | 2009 | 19443550 |
| membrane bioreactor treatment of a simulated metalworking fluid wastewater containing ethylenediaminetetraacetic acid and dicyclohexylamine. | membrane bioreactors (mbrs) have been installed at automotive plants to treat metalworking fluid (mwf) wastewaters, which are known to contain toxic and/or recalcitrant organic compounds. a laboratory study was conducted to evaluate treatment of a simulated wastewater prepared from a semisynthetic mwf, which contains two such compounds, dicyclohexylamine (dcha) and ethylenediaminetetraacetic acid (edta). primary findings were as follows: during stable operating periods, almost all chemical oxyge ... | 2009 | 19445324 |
| viability and potential for immigration of airborne bacteria from africa that reach high mountain lakes in europe. | we have analysed the diversity of the bacteria, which grow after addition of concentrated airborne particles and desert dust in different microcosms combinations with water samples from oligotrophic alpine lakes. we used, on the one hand, airborne bacteria transported by an african dust plume and collected in a high mountain area in the central pyrenees (spain). on the other hand, we collected desert dust in mauritania (c. 3000 km distance, and a few days estimated airborne journey), a known sou ... | 2009 | 19453609 |
| [effect of a novel fibrinolytic enzyme fa-i on thrombosis and thrombolysis]. | to study the effect of fa-i, a novel fibrinolytic enzyme isolated from the metabolite of arthrobacter sp. dr-536, on thrombosis and thrombolysis in vivo. | 2009 | 19462909 |
| molecular design of a nylon-6 byproduct-degrading enzyme from a carboxylesterase with a beta-lactamase fold. | a carboxylesterase with a beta-lactamase fold from arthrobacter possesses a low level of hydrolytic activity (0.023 mumol.min(-1).mg(-1)) when acting on a 6-aminohexanoate linear dimer byproduct of the nylon-6 industry (ald). g181d/h266n/d370y triple mutations in the parental esterase increased the ald-hydrolytic activity 160-fold. kinetic studies showed that the triple mutant possesses higher affinity for the substrate ald (k(m) = 2.0 mm) than the wild-type ald hydrolase from arthrobacter (k(m) ... | 2009 | 19476493 |
| microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes. | in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ... | 2009 | 19477544 |
| microbial responses to mustard gas dumped in the baltic sea. | microbiological studies were carried out on chemical weapon dump sites in the baltic sea. the effect of mustard gas hydrolysis products (mghps) on marine microbiota and the ability of microorganisms to degrade mghps were studied. many stations at the dump sites demonstrated reduced microbial diversity, and increased growth of species able to use mustard gas hydrolysis products as sole source of carbon. significant amounts of mghp-degrading bacteria were revealed in the near-bottom water. the mgh ... | 2009 | 19481794 |
| identification of a novel self-sufficient styrene monooxygenase from rhodococcus opacus 1cp. | sequence analysis of a 9-kb genomic fragment of the actinobacterium rhodococcus opacus 1cp led to identification of an open reading frame encoding a novel fusion protein, stya2b, with a putative function in styrene metabolism via styrene oxide and phenylacetic acid. gene cluster analysis indicated that the highly related fusion proteins of nocardia farcinica ifm10152 and arthrobacter aurescens tc1 are involved in a similar physiological process. whereas 413 amino acids of the n terminus of stya2 ... | 2009 | 19482928 |
| bacterial diversity of soil samples from the western himalayas, india. | high-altitude cold habitats of the himalayas are little explored with respect to bacterial diversity. diverse bacterial species and phylotypes obtained by culture-dependent and culture-independent approaches are reported here. phylogenetic analysis and modulation of bacterial diversity with altitude and available organic carbon content are also described. psychrophilic and psychrotolerant bacteria dominated the himalayan habitats, accounting for 60% of the cultivated strains. isolates produced o ... | 2009 | 19483785 |
| batch and continuous biosorption of cu(2+) by immobilized biomass of arthrobacter sp. | the ability of free and polysulphone immobilized biomass of arthrobacter sp. to remove cu(2+) ions from aqueous solution was studied in batch and continuous systems. the langmuir and freundlich isotherm models were applied to the data. the langmuir isotherm model was found to fit the sorption data indicating that sorption was monolayer and uptake capacity (q(o)) was 175.87 and 158.7mg/g for free and immobilized biomass respectively at ph 5.0 and 30 degrees c temperature, which was also confirmed ... | 2009 | 19487070 |
| glycinebetaine-induced water-stress tolerance in coda-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes. | rice (oryza sativa l.), a non-accumulator of glycinebetaine (gb), is highly susceptible to abiotic stress. transgenic rice with chloroplast-targeted choline oxidase encoded by the coda gene from arthrobacter globiformis has been evaluated for inheritance of transgene up to r5 generation and water-stress tolerance. during seedling, vegetative and reproductive stages, transgenic plants could maintain higher activity of photosystem ii and they show better physiological performance, for example, enh ... | 2009 | 19490479 |
| optimization of physical parameters for lipase production from arthrobacter sp. bgcc#490. | the physical parameters for the production of thermostable, alkaline lipase from arthrobacter sp. bgcc# 490 were optimized using response surface methodology (rsm), employing face centered central composite design (fcccd). the design was employed by selecting ph, temperature and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by rsm. a second-order quadratic model and response surface method showed that the optimum conditions for lipa ... | 2009 | 19517996 |
| two alternative modes for optimizing nylon-6 byproduct hydrolytic activity from a carboxylesterase with a beta-lactamase fold: x-ray crystallographic analysis of directly evolved 6-aminohexanoate-dimer hydrolase. | promiscuous 6-aminohexanoate-linear dimer (ald)-hydrolytic activity originally obtained in a carboxylesterase with a beta-lactamase fold was enhanced about 80-fold by directed evolution using error-prone pcr and dna shuffling. kinetic studies of the mutant enzyme (hyb-s4m94) demonstrated that the enzyme had acquired an increased affinity (k(m) = 15 mm) and turnover (k(cat) = 3.1 s(-1)) for ald, and that a catalytic center suitable for nylon-6 byproduct hydrolysis had been generated. construction ... | 2009 | 19521995 |
| synthesis of 9-beta-d-arabinofuranosylguanine by combined use of two whole cell biocatalysts. | unlike the preparation of other purine nucleosides, transglycosylation from a pyrimidine nucleoside and guanine is difficult because of the low solubility of this base. thus, another strategy, based on the coupled action of two whole cell biocatalyzed reactions, transglycosylation and deamination, was used. enterobacter gergoviae and arthrobacter oxydans were employed to synthesize 9-beta-d-arabinofuranosylguanine (arag), an efficient anti leukemic drug. | 2009 | 19523826 |
| isolation and characterization of arthrobacter sp. hy2 capable of degrading a high concentration of p-nitrophenol. | a soil bacterium strain, capable of using p-nitrophenol (pnp) as its sole source of carbon and energy, was isolated by enrichment on minimal salt medium (msm). on the basis of a phylogenetic analysis of 16s rrna gene sequences the bacterium is a species of arthrobacter, closely related to arthrobacter ureafaciens dsm 20126. this strain has an unusually high substrate tolerance for pnp degradation in msm. greatest degradation of pnp was observed at 30 degrees c and under slightly alkaline ph (ph ... | 2009 | 19540107 |
| azolla-anabaena's behaviour in urban wastewater and artificial media--influence of combined nitrogen. | the results of using the nitrogen fixing symbiotic system azolla-anabaena to improve the quality of treated urban wastewater, particularly on what concerns phosphorus removal efficiencies (40-65%), obtained in continuous assays performed during the past few years and presented earlier, were very promising. nevertheless, the presence of combined nitrogen in some wastewaters can compromise the treatment efficiency. the main goal of this work was to compare plants behaviour in wastewater and in min ... | 2009 | 19559459 |
| selection of a cold-adapted bacterium for bioremediation of wastewater at low temperatures. | amongst more than 1000 isolates collected in various cold environments, the strain arthrobacter psychrolactophilus sp 31.3 has been selected for its ability to grow and to produce exoenzymes at low temperatures, its inability to grow at 37 degrees c, its non-halophilic character and its growth versatility on various media. this non-pathogenic strain displays a strong resistance to desiccation and storage at room temperature and is suitable for the production of freeze-dried bacterial starters. w ... | 2009 | 19578929 |
| production of acrylic acid from acrylonitrile by immobilization of arthrobacter nitroguajacolicus zjutb06-99. | immobilized cells of arthrobacter nitroguajacolicus zjutb06-99 capable of producing nitrilase was used for biotransformation of acrylonitrile to acrylic acid. six different entrapment matrixes were chosen to search for a suitable support in terms of nitrilase activity. ca-alginate proved to be more advantageous over other counterparts in improvement of the biocatalyst activity and bead mechanical strength. the effects of sodium alginate concentration, cacl2 concentration, bead diameter, and rati ... | 2009 | 19597316 |
| arthrobacter psychrochitiniphilus sp. nov., a psychrotrophic bacterium isolated from antarctica. | a psychrotrophic arthrobacter strain, gp3(t), was isolated from adélie penguin guano from antarctica and characterized. the 16s rrna gene sequence of the novel strain showed the highest similarity (97.8 %) with that of arthrobacter psychrolactophilus b7(t). the novel strain showed a morphological change from rod to coccus. the growth temperature range of strain gp3(t) was 0-25 degrees c, with optimal growth at 20 degrees c. the strain grew over a salinity range of between 0 and 3 % (w/v) nacl an ... | 2009 | 19625417 |
| a new cold-adapted beta-d-galactosidase from the antarctic arthrobacter sp. 32c - gene cloning, overexpression, purification and properties. | the development of a new cold-active beta-d-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. | 2009 | 19631003 |
| monitoring enzyme catalysis in the multimeric state: direct observation of arthrobacter 4-hydroxybenzoyl-coenzyme a thioesterase catalytic complexes using time-resolved electrospray ionization mass spectrometry. | the ability to examine real-time reaction kinetics for multimeric enzymes in their native state may offer unique insights into understanding the catalytic mechanism and its interplay with three-dimensional structure. in this study, we have used a time-resolved electrospray mass spectrometry approach to probe the kinetic mechanism of 4-hydroxybenzoyl-coenzyme a (4-hba-coa) thioesterase from arthrobacter sp. strain su in the millisecond time domain. intact tetrameric complexes of 4-hba-coa thioest ... | 2009 | 19635449 |
| maturing dynamics of surface microflora in fontina pdo cheese studied by culture-dependent and -independent methods. | to study the evolution of rind microbial communities in fontina pdo cheese. | 2009 | 19054234 |
| treatment of chromium(vi) solutions in a pilot-scale bioreactor through a biofilm of arthrobacter viscosus supported on gac. | the aim of this work is to evaluate the applicability of a biofilm to the removal of chromium in solution, at a pilot scale. the effect of the initial concentration of metal on the biosorption behavior of an arthrobacter viscosus biofilm supported on granular activated carbon, in batch and column essays was also analyzed. six isotherm equations have been tested in the present study. the best fit was obtained with the freundlich model. it was observed that as the initial chromium concentration in ... | 2009 | 18565747 |
| identification and characterization of bacteria isolated under selective pressure of volatile organic compounds. | several bacterial strains tolerant to volatile organic compounds (vocs) were isolated from the air emissions of a typical industrial wood painting plant. four strains that grew vigorously on a mineral salt basal medium and voc-saturated atmosphere were identified using biolog and fatty acids analysis. a 16s rdna sequence comparison was also performed on three of the four strains. their phylogenetic positions were respectively: mc8m6 of the genus arthrobacter mcep3 of the genus microbacterium, mc ... | 2009 | 20112870 |
| bacterial diversity from benthic mats of antarctic lakes as a source of new bioactive metabolites. | during the micromat project, the bacterial diversity of microbial mats growing in the benthic environment of antarctic lakes was accessed for the discovery of novel antibiotics. in all, 723 antarctic heterotrophic bacteria belonging to novel and/or endemic taxa in the +¦-, +¦- and +¦-subclasses of the proteobacteria, the bacteroidetes branch, and of the high and low percentage g+c gram-positives, were isolated, cultivated in different media and at different temperatures, and then screened for th ... | 2009 | 21798170 |
| a novel replicative enzyme encoded by the linear arthrobacter plasmid pal1. | the soil bacterium arthrobacter nitroguajacolicus rü61a contains the linear plasmid pal1, which codes for the degradation of 2-methylquinoline. like other linear replicons of actinomycetes, pal1 is characterized by short terminal inverted-repeat sequences and terminal proteins (tppal1) covalently attached to its 5' ends. tppal1, encoded by the pal1.102 gene, interacts in vivo with the protein encoded by pal1.101. bioinformatic analysis of the pal1.101 protein, which comprises 1,707 amino acids, ... | 2010 | 20675469 |
| [isolation, identification and soil remediation of atrazine-degrading strain t3 ab1]. | to provide new atrazine-degrading strains for atrazine-polluted soil, we isolated the high-efficiency degradation bacterium from contaminated soil, identified with taxonomy, and studied the degrading characteristics and remediation capability of the strain in black soil. | 2010 | 21365918 |
| effects of bacterial treatment at early stages of atlantic cod (gadus morhua l.) on larval survival and development. | to assess the effects of bacterial treatment at the earliest stages of cod rearing on the microbial load, larval development and performance, testing three bacterial strains (carnobacterium divergens v41, arthrobacter sp. and enterococcus sp.) in vivo that were previously shown to have inhibitory potential towards fish pathogens in vitro. | 2010 | 19664064 |
| structural basis for cofactor-independent dioxygenation of n-heteroaromatic compounds at the alpha/beta-hydrolase fold. | enzymatic catalysis of oxygenation reactions in the absence of metal or organic cofactors is a considerable biochemical challenge. the co-forming 1-h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (hod) from arthrobacter nitroguajacolicus rü61a and 1-h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase (qdo) from pseudomonas putida 33/1 are homologous cofactor-independent dioxygenases involved in the breakdown of n-heteroaromatic compounds. to date, they are the only dioxygenases suggested to belong to the alp ... | 2010 | 20080731 |
| phylogenetic diversity of alkaline protease-producing psychrotrophic bacteria from glacier and cold environments of lahaul and spiti, india. | the diversity of proteolytic bacteria associated with a glacier and cold environment soils from three different locations in lahaul and spiti, india was investigated. two hundred seventeen bacterial strains were isolated in pure culture. subsequently these strains were screened for protease-production and one hundred nine showed protease production. from these protease producing psychrotrophic bacteria twenty showing high enzyme production at low temperature and alkaline ph were characterized an ... | 2010 | 20082368 |
| biotransformation of nicotine by microorganism: the case of pseudomonas spp. | several bacterial species are capable of using nicotine, the main alkaloid in tobacco plants, as a substrate for growth. the dominant species include members of two genera, pseudomonas and arthrobacter. the degradation pathway and genetic structure of nicotine catabolism in arthrobacter nicotinovorans were recently reviewed (brandsch appl microbiol biotechnol 69:493-498, 2006). here, we present up-to-date information on biodegradation of nicotine by pseudomonas spp. species in this genus capable ... | 2010 | 20091027 |
| influence of internal biofilm growth on residual permeability loss in aerobic granular membrane bioreactors. | membrane fouling results in flux decline or transmembrane pressure drop increase during membrane bioreactor (mbr) operation. physical and chemical cleanings are essential to keep an mbr operating at an appropriate membrane flux. considerable residual membrane permeability loss that cannot be removed by conventional cleaning requires membrane replacement. this study demonstrates that an internal biofilm can develop inside a hollow-fiber membrane and can probably account for up to 58.9 and 81.3% o ... | 2010 | 20102183 |
| heavy metal resistance in arthrobacter ramosus strain g2 isolated from mercuric salt-contaminated soil. | present study describes isolation of a multiple metal-resistant arthrobacter ramosus strain from mercuric salt-contaminated soil. the isolate was found to resist and bioaccumulate several metals, such as cadmium, cobalt, zinc, chromium and mercury. maximum tolerated concentrations for above metals were found to be 37, 525, 348, 1530 and 369 microm, respectively. the isolate could also reduce and detoxify redox-active metals like chromium and mercury, indicating that it has great potential in bio ... | 2010 | 20060643 |
| growth of arthrobacter sp. strain jbh1 on nitroglycerin as the sole source of carbon and nitrogen. | arthrobacter sp. strain jbh1 was isolated from nitroglycerin-contaminated soil by selective enrichment. detection of transient intermediates and simultaneous adaptation studies with potential intermediates indicated that the degradation pathway involves the conversion of nitroglycerin to glycerol via 1,2-dinitroglycerin and 1-mononitroglycerin, with concomitant release of nitrite. glycerol then serves as the source of carbon and energy. | 2010 | 20061454 |
| enhanced cyclic adenosine monophosphate production by arthrobacter a302 through rational redistribution of metabolic flux. | cyclic adenosine monophosphate (camp) was synthesized through the purine salvage synthesis pathway by arthrobacter a302. results showed that hypoxanthine was the best of the precursors, and the camp concentration reached 4.06 g/l. for inhibition of the glycolytic pathway, sodium fluoride was found the optimal effector, which was further studied on camp production. with the addition of 0.4 g/l of sodium fluoride, the maximal camp concentration reached 11.04 g/l, and the concentrations of lactic a ... | 2010 | 20064709 |
| bacterial community diversity in the brazilian atlantic forest soils. | the aim of this study was to characterize the bacterial community diversity of the brazilian atlantic forest soil by means of both cultivation and 16s rrna clone libraries. a collection of 86 representative isolates, obtained from six samples of atlantic forest soils from the national park of serra dos órgãos (parnaso), belonged to the genera arthrobacter, bacillus, burkholderia, leifsonia, paenibacillus, pseudomonas, ralstonia, serratia, and streptomyces according to the 16s rrna sequences. rep ... | 2010 | 20886336 |
| weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution. | several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ... | 2010 | 20525872 |
| influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments. | because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ... | 2010 | 20553043 |
| appraising bacterial strains for rapid bod sensing--an empirical test to identify bacterial strains capable of reliably predicting real effluent bods. | the measured response of rapid biochemical oxygen demand (bod) biosensors is often not identical to those measured using the conventional 5-day bod assay. this paper highlights the efficacy of using both glucose-glutamic acid (gga) and organisation for economic cooperation and development (oecd) bod standards as a rapid screen for microorganisms most likely to reliably predict real effluent bods when used in rapid bod devices. using these two synthetic bod standards, a microorganism was identifi ... | 2010 | 20938773 |
| structural and kinetic studies on the ser101ala variant of choline oxidase: catalysis by compromise. | the oxidation of choline catalyzed by choline oxidase includes two reductive half-reactions where fad is reduced by the alcohol substrate and by an aldehyde intermediate transiently formed in the reaction. each reductive half-reaction is followed by an oxidative half-reaction where the reduced flavin is oxidized by oxygen. here, we have used mutagenesis to prepare the ser101ala mutant of choline oxidase and have investigated the impact of this mutation on the structural and kinetic properties of ... | 2010 | 20561507 |
| high-density polyethylene (hdpe)-degrading potential bacteria from marine ecosystem of gulf of mannar, india. | assessment of high-density polyethylene (hdpe)-degrading bacteria isolated from plastic waste dumpsites of gulf of mannar. | 2010 | 20586938 |
| investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods. | water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ... | 2010 | 20587384 |
| variability of sediment-contact tests in freshwater sediments with low-level anthropogenic contamination--determination of toxicity thresholds. | freshwater sediments with low levels of anthropogenic contamination and a broad range of geochemical properties were investigated using various sediment-contact tests in order to study the natural variability and to define toxicity thresholds for the various toxicity endpoints. tests were performed with bacteria (arthrobacter globiformis), yeast (saccharomyces cerevisiae), nematodes (caenorhabditis elegans), oligochaetes (lumbriculus variegatus), higher plants (myriophyllum aquaticum), and the e ... | 2010 | 20594629 |
| prevalence of the gene trzn and biogeographic patterns among atrazine-degrading bacteria isolated from 13 colombian agricultural soils. | the following study evaluated the diversity and biogeography of 83 new atrazine-degrading bacteria and the composition of their atrazine degradation genes. these strains were isolated from 13 agricultural soils and grouped according to rep-pcr genomic fingerprinting into 11 major clusters, which showed biogeographic patterns. three clusters (54 strains) belonged to the genus arthrobacter, seven clusters (28 strains) were similar to the genus nocardioides and only one strain was a gram-negative f ... | 2010 | 20597985 |
| study on the removal of indoor vocs using biotechnology. | with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ... | 2010 | 20598440 |
| chemical composition and antibacterial activity of essential oils of lantana camara, ageratum houstonianum and eupatorium adenophorum. | essential oils have applications in folk medicine, food preservation, and as feed additives. the essential oils of lantana camara linn. (verbenaceae), ageratum houstonianum mill. (asteraceae) and eupatorium adenophorum spreng. (asteraceae) were analyzed by gas chromatography-mass spectrometry (gcms). in l. camara oil, of the total identified (83.91%) volatile constituents, five constituents [3,7,11-trimethyl-1,6,10-dodecatriene (28.86%), beta-caryophyllene (12.28%), zingiberene (7.63%), gamma-cu ... | 2010 | 20645797 |
| mechanism of boron tolerance in soil bacteria. | boron (b) is toxic to living cells at levels above a certain threshold. we isolated several b-tolerant bacterial strains from soil samples and studied them for possible mechanisms of b tolerance. 16s rrna gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 genera: arthrobacter, rhodococcus, lysinibacillus, algoriphagus, gracilibacillus, and bacillus. these isolates exhibited b-tolerance levels of 80, 100, 150, 300, 450, and 450 mmol/l, r ... | 2010 | 20130690 |
| identification and in vitro deoxynucleotidylation of the terminal protein of the linear plasmid pal1 of arthrobacter nitroguajacolicus rü61a. | the plasmid pal1 of arthrobacter nitroguajacolicus rü61a is a linear replicon, characterized by inverted terminal repeats and terminal proteins (tps) covalently bound to its 5'-ends. previous sequence analysis and predictions of possible secondary structures formed by telomeric 3'-overhangs indicated significant differences of the 'left' and 'right' telomere of pal1, raising the question of whether each terminus is recognized by a specific protein. the genes pal1.102 and pal1.103, located close ... | 2010 | 20141532 |
| the biomass yielding process of xenobiotic degradation. | yields of activated sludge and an arthrobacter sp. biomass on organic xenobiotic 2,4-dichlorophenoxyacetic acid (2,4-d) and on the intermediates of selected 2,4-d metabolism pathways were measured. activated sludge yield on 2,4-d was lower by approximately 24-45% compared to the combined yields produced separately by the lower intermediates. for activated sludge, cell synthesis only consumed 33% of the electrons generated from 2,4-d oxidation, while the other 67% were used for energy. the high e ... | 2010 | 20153175 |
| one-pot conversion of levan prepared from serratia levanicum nn to difructose anhydride iv by arthrobacter nicotinovorans levan fructotransferase. | the newly established difructose anhydride iv (dfa iv) production system is comprised of the effective production of levan from sucrose by serratia levanicum nn, the conversion of the levan into dfa iv by levan fructotransferase from arthrobacter nicotinovorans gs-9, which is highly expressed in an escherichiacoli transformant, and a practical purification step. the chemical properties of dfa iv were also investigated. | 2010 | 20159571 |
| biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. | marine biosphere offers wealthy flora and fauna, which represents a vast natural resource of imperative functional commercial grade products. among the various bioactive compounds, biosurfactant (bs)/bioemulsifiers (be) are attracting major interest and attention due to their structural and functional diversity. the versatile properties of surface active molecules find numerous applications in various industries. marine microorganisms such as acinetobacter, arthrobacter, pseudomonas, halomonas, ... | 2010 | 20172021 |
| c, n, and h isotope fractionation of the herbicide isoproturon reflects different microbial transformation pathways. | the fate of pesticides in the subsurface is of great interest to the public, industry, and regulatory authorities. compound-specific isotope analysis (csia) is a promising tool complementary to existing methods for elucidating pesticide degradation reactions. here, we address three different initial biotransformation reactions of the phenylurea herbicide isoproturon (3-(4-isopropylphenyl)-1,1-dimethylurea) in pure culture experiments with bacterial and fungal strains. when analyzing isotopic cha ... | 2010 | 20184312 |
| role of valine 464 in the flavin oxidation reaction catalyzed by choline oxidase. | the oxidation of reduced flavin cofactors by oxygen is a very important reaction that is central to the chemical versatility of hundreds of flavoproteins classified as monooxygenases and oxidases. these enzymes are characterized by bimolecular rate constants >or=10(5) m(-1) s(-1) and produce water and hydrogen peroxide, respectively. a hydrophobic cavity close to the reactive flavin c(4a) atom has been previously identified in the 3d structure of monooxygenases but not in flavoprotein oxidases. ... | 2010 | 20184377 |
| crystallization and preliminary crystallographic analysis of manganese(ii)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from bacillus sp. jf8. | a thermostable manganese(ii)-dependent 2,3-dihydroxybiphenyl-1,2-dioxygenase derived from bacillus sp. jf8 was crystallized. the initial screening for crystallization was performed by the sitting-drop vapour-diffusion method using a crystallization robot, resulting in the growth of two crystal forms. the first crystal belonged to space group p1, with unit-cell parameters a = 62.7, b = 71.4, c = 93.6 a, alpha = 71.2, beta = 81.0, gamma = 64.0 degrees, and diffracted to 1.3 a resolution. the secon ... | 2010 | 20208161 |
| strains of internal biofilm in aerobic granular membrane bioreactors. | this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ... | 2010 | 20306189 |
| esmond e. snell--the pathfinder of b vitamins and cofactors. | esmond e. snell (1914-2003) was a giant of b-vitamin and enzyme research. his early research in bacterial nutrition had lead to the discovery of vitamins such as lipoic acid and folic acid, and an anti-vitamin avidin. he developed microbiological assay methods for riboflavin and other vitamins and amino acids, which are still used today. he also investigated the metabolism of vitamins, discovered pyridoxal and pyridoxamine as the active forms of vitamin b(6) and revealed the mechanism of transam ... | 2010 | 20353954 |
| arthrobacter sp. strain ku001 isolated from a thai soil degrades atrazine in the presence of inorganic nitrogen sources. | an atrazine degrading bacterium, strain ku001, was obtained from a sugarcane field at the cane and sugar research and development center at the kasetsart university, kamphaeng saen campus, thailand. strain ku001 had a rod-to-coccus morphological cycle during growth. biolog carbon source analysis indicated that the isolated bacterium was arthrobacter histidinolovorans. sequence analysis of pcr product indicated the 16s rrna gene in strain ku001 was 99% identical to the same region in arthrobacter ... | 2010 | 20372034 |
| aerobic biodegradation of a sulfonated phenylazonaphthol dye by a bacterial community immobilized in a multistage packed-bed bac reactor. | a microbial community able to aerobically degrade the azo dye acid orange 7 was selected from riparian or lacustrine sediments collected at sites receiving textile wastewaters. three bacterial strains, pertaining to the genera pseudomonas, arthrobacter, and rhizobium, constitute the selected community. the biodegradation of ao7 was carried out in batch-suspended cell culture and in a continuously operated multistage packed-bed bac reactor. the rapid decolorization observed in batch culture, join ... | 2010 | 20376575 |
| structural characterization of the major glycolipids from arthrobacter globiformis and arthrobacter scleromae. | arthrobacter is a genus of gram-positive bacteria widely distributed in soil. the ability to catabolize a variety of xenobiotics has shown their potential as a detoxifying agent. recently, arthrobacter has been also recognized as an opportunistic pathogen. glycolipids from a. scleromae, a clinical isolate, and a. globiformis, from soil, were isolated by chloroform-methanol extraction and subsequently purified using column chromatography and high-performance liquid chromatography. structural stud ... | 2010 | 20381794 |
| culture-dependent and culture-independent diversity of actinobacteria associated with the marine sponge hymeniacidon perleve from the south china sea. | in this report, the diversity of actinobacteria associated with the marine sponge hymeniacidon perleve collected from a remote island of the south china sea was investigated employing classical cultivation and characterization, 16s rdna library construction, 16s rdna-restriction fragment length polymorphism (rdna-rflp) and phylogenetic analysis. a total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological characteristics for phyl ... | 2010 | 20383659 |
| aepa of pectobacterium is not involved in the regulation of extracellular plant cell wall degrading enzymes production. | plant cell wall degrading enzymes (pcwde) are the major virulence determinants in phytopathogenic pectobacterium, and their production is controlled by many regulatory factors. in this study, we focus on the role of the aepa protein, which was previously described to be a global regulator of pcwde production in pectobacterium carotovorum (murata et al. in mol plant microbe interact 4:239-246, 1991). our results show that neither inactivation nor overexpression of aepa affects pcwde production in ... | 2010 | 20386924 |
| [isolation and identification of a fibrinolytic bacterium strain from frozen soil in the tibetan plateau]. | cardiovascular disease, harmful to human health, is a worldwide disease, for which fibrinolytic enzyme is usually used as an effective remedy. fibrinolytic microbes under extreme environmental conditions may excrete some high efficient and safe fibrinolytic enzymes. so, for obtaining this fibrinolytic enzyme, we isolated fibrinolytic strains from frozen soil in the tibetan plateau. | 2010 | 20387455 |
| glycosylation patterns of human alpha2-macroglobulin: analysis of lectin binding by electron microscopy. | human alpha2-macroglobulin (alpha 2m) is a 720 kda glycoprotein that presents two ultrastructural conformations: slow (s-alpha 2m) and fast (f-alpha 2m). alpha 2m acts mainly as a proteinase scavenger, but an immunomodulatory role was also proposed. this work studies the effect of desialylation and deglycosylation on the structure patterns of alpha 2m by ultrastructural analysis of lectin-induced aggregates, which represents a new approach that had never been previously used. transmission electr ... | 2010 | 20399104 |
| comparative study of properties of immobilized lipase onto glutaraldehyde-activated amino-silica gel via different methods. | the enzyme-aggregate coating method was performed to immobilize arthrobacter sp. lipase in order to achieve better catalytic properties comparable to the conventional covalent attachment and covalent attachment plus cross-linking. the glutaraldehyde-activated amino-silica gel which was synthesized by sol-gel technique was used as the support, and the catalytic characteristics of the lipase preparations were tested in the asymmetric acylation of 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-o ... | 2010 | 20399626 |
| homology modeling and docking study of xanthine oxidase of arthrobacter sp. xl26. | hyperuricemia is a condition of defective purine metabolism characterized with elevated serum uric acid (ua) level that further leads to gout and gouty nephrolithiasis disorders. gout is a world wide distributed rheumatic disease comprises 1% of the total population and still is in increasing state. one of the factors contributing to overproduction of ua is the hydroxylation of xanthine catalyzed by xanthine oxidase (xo). in the present study, 3d modeling of arthrobacter sp. xl26 (xodb) protein ... | 2010 | 20403378 |
| rescuing of the hydride transfer reaction in the glu312asp variant of choline oxidase by a substrate analogue. | in the active site of choline oxidase, glu312 participates in binding the trimethylammonium group of choline, thereby positioning the alcohol substrate properly for efficient hydride transfer to the enzyme-bound flavin. previous studies have shown that substitution of glu312 with aspartate results in a perturbed mechanism of hydride transfer, with a 260-fold decrease in the rate associated with the mutation. here, the reaction of alcohol oxidation catalyzed by the glu312asp enzyme has been inves ... | 2010 | 20447376 |
| the microbe-mineral environment and gypsum neogenesis in a weathered polar evaporite. | evaporitic deposits are a globally widespread habitat for micro-organisms. the microbe-mineral environment in weathered and remobilized gypsum from exposed mid-ordovician marine evaporite beds in the polar desert of devon island, nunavut, canadian high arctic was examined. the gypsum is characterized by internal green zones of cyanobacterial colonization (dominated by gloeocapsa/aphanothece and chroococcidiopsis spp. morphotypes) and abundant black zones, visible from the surface, that contain p ... | 2010 | 20456500 |
| the type of cheese curds determined the colouring capacity of brevibacterium and arthrobacter species. | this study compares the colouring capacity of brevibacterium aurantiacum (ba), brevibacterium bl and arthrobacter species as in relation to deacidified media made from lactic curd (epoisses), mixed curds (munster) and rennet curds (livarot or reblochon). ba colouring capacity proved to be constant, leading to a dark orange colour, irrespective of the deacidified media. however, it gave too dark a colour for reblochon. the strains bl and as were not adapted to the colouring of epoisses deacidifie ... | 2010 | 20462468 |
| calcite-forming bacteria for compressive strength improvement in mortar. | microbiological calcium carbonate precipitation (mcp) has been investigated for its ability to improve the compressive strength of concrete mortar. however, very few studies have been conducted on the use of calcite-forming bacteria (cfb) to improve compressive strength. in this study, we discovered new bacterial genera that are capable of improving the compressive strength of concrete mortar. we isolated 4 cfb from 7 environmental concrete structures. using sequence analysis of the 16s rrna gen ... | 2010 | 20467254 |