Publications

partial purification and chemical characterization of a glycoprotein (putative hydrocolloid) emulsifier produced by a marine bacterium antarctobacter.	during screening for novel emulsifiers and surfactants, a marine alphaproteobacterium, antarctobacter sp. tg22, was isolated and selected for its production of an extracellular emulsifying agent, ae22. this emulsifier was produced optimally in a low-nutrient seawater medium supplemented with glucose and was extractable by cold ethanol precipitation of the high-molecular-weight fraction (>100 kda). production of ae22 commenced towards the late exponential phase of growth, with maximum emulsifying ...	2007	17641887
[phylogenetic diversity of culturable bacteria in the ancient salt deposits of the yipinglang salt mine, p. r. china].	the microbial diversity of cultivable bacteria, isolated from the ancient salt deposits from the yipinglang salt mine (ypl) in the yunnan province, p. r. china,was investigated by using conventional culture-dependent method and phylogenetic analyses based on 16s rrna gene sequence comparisons. 38 bacteria strains were isolated from the brine, halite and saline soil samples on mba (marine broth agar 2216, difco) and isp 2 (international streotomyces project medium 2) media supplemented with 0.5-3 ...	2007	17944352
[obtaining and characterization of dna-containing micromummies of yeasts and gram-positive bacteria with enhanced cell wall permeability: application in pcr].	the procedure of obtaining dna-containing cell envelopes ("micromummies") of bacteria, yeasts, and fungi using chaotropic salts has been developed previously and the possibility of their direct application in pcr has been demonstrated. the fine structure of micromummies has been studied by electron microscopic methods. this work has demonstrated that additional treatment of micromummies of yeasts and gram-positive bacteria with proteinase k results in hydrolytic degradation of cell proteins and ...	2007	17410877
biodegradative potential and characterization of psychrotolerant polychlorinated biphenyl-degrading marine bacteria isolated from a coastal station in the terra nova bay (ross sea, antarctica).	antarctic marine bacteria were screened for their ability to degrade polychlorinated biphenyls (pcb) as the sole carbon and energy source at both 4 degrees c and 15 degrees c. pcb-degrading isolates (7.1%) were identified by sequencing their 16s rdna as pseudoalteromonas, psychrobacter and arthrobacter members. one representative isolate per genera was selected for evaluating the biodegradative potential under laboratory scale and phenotypically characterized. removal of individual pcb congeners ...	2007	17854841
degradation of pentachlorophenol by pure and mixed cultures in two different soils.	pentachlorophenol (pcp) is the second highest volume pesticide used in the united states. it is a mutagenic compound whose exposure poses significant health effects, one of the most desirable, environmentally friendly treatment methods is bioremediation. for soil-based contamination, the effectiveness of bioremediation will also be affected by the presence of an active indigenous population, sorption of the contaminant onto the soil, and environmental parameters.	2007	17668821
enzymatic synthesis of cephalosporins. the immobilized acylase from arthrobacter viscosus: a new useful biocatalyst.	the acylase from arthrobacter viscosus was immobilized, studied in the enzymatic synthesis of some cephalosporins by kinetically controlled n-acylation (kcna) of different cephem nuclei, and compared with the penicillin g acylase (pga) from escherichia coli. the reaction outcomes were dependent on the acylase microbial source and on the type of immobilization support. generally, both enzymes, when immobilized onto hydrophilic resins such as glyoxyl-agarose (activated with aldehyde groups), displ ...	2007	17879093
two bacterial entophytes eliciting both plant growth promotion and plant defense on pepper (capsicum annuum l.).	plant growth-promoting rhizobacteria (pgpr) have the potential to be used as microbial inoculants to reduce disease incidence and severity and to increase crop yield. some of the pgpr have been reported to be able to enter plant tissues and establish endophytic populations. here, we demonstrated an approach to screen bacterial endophytes that have the capacity to promote the growth of pepper seedlings and protect pepper plants against a bacterial pathogen. initially, out of 150 bacterial isolate ...	2007	18051359
[study on the atrazine-degrading genes in arthrobacter sp. ag1].	atrazine could be used as the sole carbon, nitrogen and energy sources for growth by strain arthrobacter sp. ag1, and the atrazine-degrading genes of ag1 were found to be the combination of trzn, atzb and atzc. the atrazine chloride hydrolysase gene trzn was cloned by pcr amplification,whose sequence shared 99% identity with that of norcardioides sp. c190. two large plasmids were found in ag1,and trzn and atzb were confirmed to be localized on the larger plasmid pag1 by the method of southern hy ...	2007	18051853
[screening and function analysis of a cyclohexanone-degrading bacterium cn1 from deep sea sediment].	micrococcu luteus cn1 was found to be able to utilize cyclohexanone well from the strains originally isolated from pacific ocean sediment. the optimum conditions for its growth were determined as 25 degrees c -37 degrees c, ph 8, salinity 6%. it could survive in the medium with high concentration of cyclohexanone ( > 44% v/v), and grew most vigorously in medium with 16.7% (v/v) cyclohexanone. cn1 could transform cyclohexanol to cyclohexanone which could be further degraded and mineralized quickl ...	2007	18062257
phylogenetic diversity of bacteria isolates from the qinghai-tibet plateau permafrost region.	the qinghai-tibet plateau in east asia is a unique and important permafrost environment. however, its microbiology remains largely unexplored to date. in this study, sediment samples were collected from the qinghai-tibet plateau permafrost region, bacteria isolation procedures were performed 8 times, and the samples incubated at 4 degrees c for nearly 3 months. the number of colony forming units (cfu) ranged from 0 to 10(7)/(g dry soil). the quantity of culturable bacteria grew exponentially wit ...	2007	17898857
cloning, expression, and characterization of bacillus sp. snu-7 inulin fructotransferase.	a gene encoding inulin fructotransferase (di-d-fructofuranose 1,2': 2,3' dianhydride [dfa iii]-producing iftase, ec 4.2.2.18) from bacillus sp. snu-7 was cloned. this gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a 410-amino acid mature protein. the deduced amino acid sequence was 98% identical to arthrobacter globiformis c11-1 iftase (dfa iii-producing). the enzyme was successfully expressed in e. coli as a functiona ...	2007	18051351
facultative but persistent trans-generational immunity via the mother's eggs in bumblebees.	it is widely acknowledged that a mother, and the maternal environment, can have a profound effect on the phenotype of her offspring. for example, immunocompetent vertebrate mothers can pass on immune factors to their otherwise immune naïve offspring. recently, it has also been demonstrated in invertebrates that maternal contact with pathogens and immune elicitors can have a positive impact on offspring resistance and immune system components. here, using a cross-fostering design, we show that tr ...	2007	18088585
arthrobacter subterraneus sp. nov., isolated from deep subsurface water of the south coast of korea.	strain ch7t, a pale yellow-pigmented bacterium and new isolate from deep subsurface water of the south coast of korea, was subjected to a polyphasic taxonomic study. ch7t grew between 5 and 37 degrees c, ph 5.3-10.5, and tolerated up to 13% nacl. a phylogenetic analysis based on 16s rrna gene sequences showed that strain ch7t was associated with the genus arthrobacter and phylogenetically closely related to the type strains arthrobacter tumbae (99.4%) and arthrobacter parietis (99.1%). however, ...	2007	18092474
[effect of quinocitrinines from the fungus penicillium citrinum on the respiration of yeasts and bacteria].	we studied the effect of quinocitrinines on the respiratory activity of yeasts (yarrowia lipolytica) and bacteria (arthrobacter globiformis). quinocitrinines were shown to activate respiration of native cells in both types of organisms. studies of yeast mitochondria showed that quinocitrinine exerts an uncoupling effect on oxidative phosphorylation, which activates the respiration, reduces the respiratory control, and decreases the adp/o ratio. experiments with intact mitochondria and native cel ...	2007	18173114
[isolation and degrading characters of chlorpyrifos degrading bacteria xz-3].	a strain of bacterium xz-3 capable of highly degrading chlorpyrifos was screened from the soil sample collected from a pesticide plant after taming and enrichment. based on analysis of phenotype, physiological and biochemical characters and 16s rdna, xz-3 was identified as arthrobacter sp.. the effects of carbon source, ph, temperature and chlorpyrifos concentration on degradation were determined. the growth of bacteria in culture media were measured by visible absorption spectrophotometry under ...	2007	18290445
strong impact on the polycyclic aromatic hydrocarbon (pah)-degrading community of a pah-polluted soil but marginal effect on pah degradation when priming with bioremediated soil dominated by mycobacteria.	bioaugmentation of soil polluted with polycyclic aromatic hydrocarbons (pahs) is often disappointing because of the low survival rate and low activity of the introduced degrader bacteria. we therefore investigated the possibility of priming pah degradation in soil by adding 2% of bioremediated soil with a high capacity for pah degradation. the culturable pah-degrading community of the bioremediated primer soil was dominated by mycobacterium spp. a microcosm containing pristine soil artificially ...	2007	17209064
solar-based detoxification of phenol and p-nitrophenol by sequential tio2 photocatalysis and photosynthetically aerated biological treatment.	simulated solar uv/tio(2) photocatalysis was efficient to detoxify a mixture of 100 mgphenoll(-1) and 50 mgp-nitrophenol (pnp) l(-1) and allow the subsequent biodegradation of the remaining pollutants and their photocatalytic products under photosynthetic aeration with chlorella vulgaris. photocatalytic degradation of phenol and pnp was well described by pseudo-first order kinetics (r(2)>0.98) with removal rate constants of 1.9x10(-4) and 2.8x10(-4)min(-1), respectively, when the pollutants were ...	2007	17350074
[function and structure of nad kinase: the key enzyme for biosynthesis of nadp (h)].		2007	17352189
isolation and characterization of four gram-positive nickel-tolerant microorganisms from contaminated sediments.	microbial communities from riparian sediments contaminated with high levels of ni and u were examined for metal-tolerant microorganisms. isolation of four aerobic ni-tolerant, gram-positive heterotrophic bacteria indicated selection pressure from ni. these isolates were identified as arthrobacter oxydans nr-1, streptomyces galbus nr-2, streptomyces aureofaciens nr-3, and kitasatospora cystarginea nr-4 based on partial 16s rdna sequences. a functional gene microarray containing gene probes for fu ...	2007	17404787
cloning and characterization of the gene encoding endo-beta-1,3-glucanase from arthrobacter sp. nhb-10.	the glua gene, encoding an endo-beta-1,3-glucanase from arthrobacter sp. (strain nhb-10), was cloned and analyzed. the deduced endo-beta-1,3-glucanase amino acid sequence was 750 amino acids long and contained a 42 amino acid signal peptide with a mature protein of 708 amino acids. there was no similarity to known endo-beta-1,3-glucanases, but glua was partially similar to two fungal exo-beta-1,3-glucanases in glycoside hydrolase (gh) family 55. of five possible residues for catalysis and two mo ...	2007	17587693
esta from arthrobacter nitroguajacolicus rü61a, a thermo- and solvent-tolerant carboxylesterase related to class c beta-lactamases.	the esta gene encoding a novel cytoplasmic carboxylesterase from arthrobacter nitroguajacolicus rü61a was expressed in escherichia coli. sequence analysis and secondary structure predictions suggested that esta belongs to the family viii esterases, which are related to class c beta-lactamases. the s-x-x-k motif that in beta-lactamases contains the catalytic nucleophile, and a putative active-site tyrosine residue are conserved in esta. the native molecular mass of hexahistidine-tagged (his6) est ...	2007	17294326
the characteristics of rhizosphere microbes associated with plants in arsenic-contaminated soils from cattle dip sites.	soil microorganisms and plants were studied in samples of arsenic-contaminated soil from two cattle dip sites. the aim was to delineate the parameters that will determine the feasibility of future remediation by growing arsenic-accumulating plants, including the identity and characteristics of some rhizosphere soil microbes. the soil samples contained high total, but low soluble arsenic concentrations which, together with other properties, resembled the previously reported characteristics of dip ...	2007	17407787
microbially mediated cadmium sorption/desorption processes in soil amended with sewage sludge.	a multi-compartment system was used to study the importance of microorganisms for cd desorption from soil amended with sewage sludge and simultaneous resorption of the mobilized metal by soil constituents. using this system made it possible to study the participation of microorganisms (arthrobacter, trichoderma), montmorillonite, humic acids, and iron oxides in resorption of the released cd. a filter-sterilized water extract of root-free soil of ph 6.7 (rf) or rf supplemented with glucose (rfg) ...	2007	17182077
nod2/card15 disease associations other than crohn's disease.	at this moment, few confirmed associations between nod2 mutations and diseases other than crohn's disease (cd) and blau syndrome (bs) have been reported, but research is ongoing in several fields where a genetic susceptibility factor and/or a role for the innate immune system is suspected. whether the crohn's-associated card15 mutations lead to a loss or gain of function of the nod2 receptor is subject to controversy, and by which mechanisms this change in function might increase the susceptibil ...	2007	17206682
amperometric biosensor for the determination of creatine.	an amperometric biosensor for the determination of creatine was developed. the carbon rod electrode surface was coated with sarcosine oxidase (sox) and creatine amidinohydrolase by cross-linking under glutaraldehyde vapour. the sox from arthrobacter sp. 1-1 n was purified and previously used for creation of a creatine biosensor. the natural sox electron acceptor, oxygen, was replaced by an [fe(cn)(6)](3-) /[ fe(cn)(6)](4-) redox mediating system, which allowed amperometric detection of an analyt ...	2007	17221239
overproduction and characterization of a recombinant d-amino acid oxidase from arthrobacter protophormiae.	a screening of soil samples for d-amino acid oxidase (d-aao) activity led to the isolation and identification of the gram-positive bacterium arthrobacter protophormiae. after purification of the wild-type d-aao, the gene sequence was determined and designated dao. an alignment of the deduced primary structure with eukaryotic d-aaos and d-aspartate oxidases showed that the d-aao from a. protophormiae contains five of six conserved regions; the c-terminal type 1 peroxisomal targeting signal that i ...	2007	17279391
expression of ksdd gene encoding 3-ketosteroid-delta1-dehydrogenase from arthrobacter simplex in bacillus subtilis.	to improve ksdh enzyme activity and the transformation level for androst-4-ene-3,17-dione.	2007	17451526
overexpression and functional analysis of cold-active beta-galactosidase from arthrobacter psychrolactophilus strain f2.	cold-active beta-galactosidase from arthrobacter psychrolactophilus strain f2 was overexpressed in escherichia coli using the cold expression system and the recombinant enzyme, rbglap, was characterized. the purified rbglap exhibited similar enzymatic properties to the native enzyme, e.g., (i) it had high activity at 0 degrees c, (ii) its optimum temperature and ph were 10 degrees c and 8.0, respectively, and (iii) it was possible to rapidly inactivate the rbglap at 50 degrees c in 5 min. moreov ...	2007	17459724
an nad(p)h-nicotine blue oxidoreductase is part of the nicotine regulon and may protect arthrobacter nicotinovorans from oxidative stress during nicotine catabolism.	an nad(p)h-nicotine blue (quinone) oxidoreductase was discovered as a member of the nicotine catabolic pathway of arthrobacter nicotinovorans. transcriptional analysis and electromobility shift assays showed that the enzyme gene was expressed in a nicotine-dependent manner under the control of the transcriptional activator pmfr and thus was part of the nicotine regulon of a. nicotinovorans. the flavin mononucleotide-containing enzyme uses nadh and, with lower efficiency, nadph to reduce, by a tw ...	2007	17293530
variations in t-rflp profiles with differing chemistries of fluorescent dyes used for labeling the pcr primers.	culture independent molecular methods have emerged as indispensable tools for studying microbial community structure and dynamics in natural habitats, since they allow a closer look at microbial diversity that is not reflected by culturing techniques. terminal restriction fragment length polymorphism (t-rflp) analysis is one of the informative and widely used techniques for such studies. however, the method has a few limitations to predict microbial community structure with significant accuracy. ...	2007	17196691
adaptation of the psychrotroph arthrobacter chlorophenolicus a6 to growth temperature and the presence of phenols by changes in the anteiso/iso ratio of branched fatty acids.	arthrobacter chlorophenolicus is a previously described gram-positive bacterium capable of degrading high concentrations of several phenolic compounds under optimal mesophilic (28 degrees c) as well as psychrophilic (5 degrees c) conditions. however, the exact mechanisms by which this organism is able to tolerate such extremes in temperature and high levels of toxic compounds are currently not known. in this study, we monitored changes in the fatty acid composition of the cell membrane under dif ...	2007	17233723
structure and action of a c-c bond cleaving alpha/beta-hydrolase involved in nicotine degradation.	the enzyme 2,6-dihydroxy-pseudo-oxynicotine hydrolase from the nicotine-degradation pathway of arthrobacter nicotinovorans was crystallized and the structure was determined by an x-ray diffraction analysis at 2.1 a resolution. the enzyme belongs to the alpha/beta-hydrolase family as derived from the chain-fold and from the presence of a catalytic triad with its oxyanion hole at the common position. this relationship assigns a pocket lined by the catalytic triad as the active center. the asymmetr ...	2007	17275835
heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in ni-rich serpentine soil and in the rhizosphere of alyssum murale.	forty-six bacterial cultures, including one culture collection strain, thirty from the rhizosphere of alyssum murale and fifteen from ni-rich soil, were tested for their ability to tolerate arsenate, cadmium, chromium, zinc, mercury, lead, cobalt, copper, and nickel in their growth medium. the resistance patterns, expressed as minimum inhibitory concentrations, for all cultures to the nine different metal ions were surveyed by using the agar dilution method. a large number of the cultures were r ...	2007	17276484
cloning of the arthrobacter sp. fg1 dehalogenase genes and construction of hybrid pathways in pseudomonas putida strains.	an arthrobacter strain, able to utilize 4-chlorobenzoic acid as the sole carbon and energy source, was isolated and characterized. the first step of the catabolic pathway was found to proceed via a hydrolytic dehalogenation that leads to the formation of 4-hydroxybenzoic acid. the dehalogenase encoding genes (fcb) were sequenced and found highly homologous to and organized as those of other 4-chlorobenzoic acid degrading arthrobacter strains. the fcb genes were cloned and successfully expressed ...	2007	17384944
chlamydomonas reinhardtii, a model system for functional validation of abiotic stress responsive genes.	stress tolerance is a multigenic character and there are many stress responsive genes, which are stress specific. although many of these have been cloned, their functional significance remains fragmentary. hence it is important to identify the relevant stress genes involved in altering the metabolism for adaptation. overexpression is one of the several approaches and chlamydomonas is a suitable system to study the functional relevance of stress genes. stress responses can only be assessed on pri ...	2007	17431668
[lignite microorganisms].	the first demonstration that samples of lignite at a depth of 10 m are considerably enriched in bacteria is reported. according to direct microscopy, the abundance of bacteria was about 10(7) cells/g. about 70% of cells had intact cell membranes and small size, which points to their anabiotic state. the fungal mycelium length was no more than 1 m. lignite inoculation onto solid glucose-yeast-peptone medium allowed as to isolate bacteria of the genera bacillus, rhodococcus, arthrobacter, micrococ ...	2007	17668464
growth and physiological response of arthrobacter protophormiae rkj100 toward higher concentrations of o-nitrobenzoate and p-hydroxybenzoate.	bioremediation of sites that are heavily contaminated with pollutant chemicals is a challenge as most of the microorganisms cannot tolerate higher concentrations of toxic compounds. only a few strains of the genus pseudomonas have been studied for their tolerance toward the higher concentrations of aromatic pollutant compounds, a phenomenon that is accompanied by various physiological changes. in the present study we have characterized the growth response and physiological changes (adaptations) ...	2007	17391368
utilization and degradation of imazaquin by a naturally occurring isolate of arthrobacter crystallopoietes.	a species of bacteria that is capable of utilizing imazaquin as the sole carbon source was isolated from soil with repeated imazaquin applications, and was identified as arthrobacter crystallopoietes (designated as strain "wwx-1"). this isolate degrades imazaquin as high as 200 microg ml-1, and the estimated dissipation half-lives increased from 1.51 d for the treatment at 50 microg ml-1 to 4.75 d for 200 microg ml-1. optimal growth of wwx-1 in mineral salt medium with 50 microg ml-1 imazaquin w ...	2007	17267013
mineralization of diuron [3-(3,4-dichlorophenyl)-1, 1-dimethylurea] by co-immobilized arthrobacter sp. and delftia acidovorans.	mineralization of diuron has not been previously demonstrated despite the availability of some bacteria to degrade diuron into 3,4-dichloroaniline (3,4-dca) and others that can mineralize 3,4-dca. a bacterial co-culture of arthrobacter sp. n4 and delftia acidovorans w34, which respectively degraded diuron (20 mg l(-1)) to 3,4-dca and mineralized 3,4-dca, were able to mineralize diuron. total diuron mineralization (20 mg l(-1)) was achieved with free cells in co-culture. when the bacteria were im ...	2007	17333465
complete nucleotide sequence of the 113-kilobase linear catabolic plasmid pal1 of arthrobacter nitroguajacolicus rü61a and transcriptional analysis of genes involved in quinaldine degradation.	the nucleotide sequence of the linear catabolic plasmid pal1 from the 2-methylquinoline (quinaldine)-degrading strain arthrobacter nitroguajacolicus rü61a comprises 112,992 bp. a total of 103 open reading frames (orfs) were identified on pal1, 49 of which had no annotatable function. the orfs were assigned to the following functional groups: (i) catabolism of quinaldine and anthranilate, (ii) conjugation, and (iii) plasmid maintenance and dna replication and repair. the genes for conversion of q ...	2007	17337569
production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses.	twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ...	2007	17701035
[a model study of pesticide biodegradation in soil].	this study addresses the efficiency of microbial preparations to degrade pesticide residues in soil. a method to degrade pesticides dnoc and pendimethalin using pseudomonas and arthrobacter bacteria with a fertilizer is described.	2007	17352205
a two-component small multidrug resistance pump functions as a metabolic valve during nicotine catabolism by arthrobacter nicotinovorans.	the genes nepab of a small multidrug resistance (smr) pump were identified as part of the pao1-encoded nicotine regulon responsible for nicotine catabolism in arthrobacter nicotinovorans. when [(14)c]nicotine was added to the growth medium the bacteria exported the (14)c-labelled end product of nicotine catabolism, methylamine. in the presence of the proton-motive force inhibitors 2,4-dinitrophenol (dnp), carbonyl cyanide m-chlorophenylhydrazone (cccp) or the proton ionophore nigericin, export o ...	2007	17464069
an internal equilibrium preorganizes the enzyme-substrate complex for hydride tunneling in choline oxidase.	the hydride transfer reaction catalyzed by choline oxidase under irreversible regime, i.e., at saturating oxygen, was shown in a recent study to occur quantum mechanically within a highly preorganized active site, with the reactive configuration for hydride tunneling being minimally affected by environmental vibrations of the reaction coordinate other than those affecting the distance between the alpha-carbon of the choline alkoxide substrate and the n(5) atom of the enzyme-bound flavin cofactor ...	2007	17472346
biotransformation of d-methionine into l-methionine in the cascade of four enzymes.	d-methionine was converted to l-methionine in a reaction system where four enzymes were used. d-amino acid oxidase (d-aao) from arthrobacter protophormiae was used for the complete conversion of d-methionine to 2-oxo-4-methylthiobutyric acid. catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation. in the second reaction step, l-phenylalanine dehydrogenase (l-phedh) from rhodococcus sp. was used to convert 2- oxo-4-methylthiobutyric acid to l-methionine, and formate dehydrog ...	2007	17534960
a novel strategy for enzymatic synthesis of 4-hydroxyisoleucine: identification of an enzyme possessing hmkp (4-hydroxy-3-methyl-2-keto-pentanoate) aldolase activity.	a two-step enzymatic synthesis process of 4-hydroxyisoleucine is suggested. in the first step, the aldol condensation of acetaldehyde and alpha-ketobutyrate catalyzed by specific aldolase results in the formation of 4-hydroxy-3-methyl-2-keto-pentanoate (hmkp). in the second step, amination of hmkp by the branched-chain amino acid aminotransferase leads to synthesis of 4-hydroxyisoleucine. an enzyme possessing hmkp aldolase activity (ashpal) was purified 2500-fold from a crude extract of arthroba ...	2007	17559390
crystallization and diffraction data of 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase: a cofactor-free oxygenase of the alpha/beta-hydrolase family.	1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase (qdo) from pseudomonas putida 33/1 catalyses the oxygenolysis of 1h-3-hydroxy-4-oxoquinoline to form n-formylanthranilic acid and carbon monoxide without the aid of cofactors. both n-terminally his6-tagged and native qdo were overexpressed in escherichia coli and purified by conventional chromatographic procedures. untagged qdo, but not his6-tagged qdo, was crystallized by the vapour-diffusion method, giving hexagonal bipyramid crystals belonging to sp ...	2007	17565175
crystallization and preliminary x-ray analysis of 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from arthrobacter nitroguajacolicus rü61a: a cofactor-devoid dioxygenase of the alpha/beta-hydrolase-fold superfamily.	1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (hod) is a cofactor-devoid dioxygenase that is involved in the anthranilate pathway of quinaldine degradation. hod has been proposed to belong to the alpha/beta-hydrolase-fold superfamily of enzymes. n-terminally his6-tagged hod has been crystallized by the hanging-drop vapour-diffusion method using sodium/potassium tartrate as a precipitant and cucl2 as an additive. the structure was solved by the single anomalous dispersion (sad) technique using dat ...	2007	17565176
characterization of aerobic bacterial and fungal microbiota on surfaces of historic scottish monuments.	twenty samples were taken from the inner or outer surfaces of stone monuments of six historic scottish buildings and ruins. biofilms developing on mineral substrates were analysed by in situ scanning electron microscopy and cultivation. various methods were used to characterize the isolates including automated ribotyping, rapd and sequencing of the 16s rrna gene for bacteria, and stereomicroscopy and sequencing of the internal transcribed spacers (its) for fungi. most samples contained microbes ...	2007	17583461
stability of microbial communities in goat milk during a lactation year: molecular approaches.	the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...	2007	17604934
glycinebetaine accumulation is more effective in chloroplasts than in the cytosol for protecting transgenic tomato plants against abiotic stress.	tomato (lycopersicon esculentum mill. cv. moneymaker) plants were transformed with a gene for choline oxidase (coda) from arthrobacter globiformis. the gene product (coda) was targeted to the chloroplasts (chl-coda), cytosol (cyt-coda) or both compartments simultaneously (chlcyt-coda). these three transgenic plant types accumulated different amounts and proportions of glycinebetaine (gb) in their chloroplasts and cytosol. targeting coda to either the cytosol or both compartments simultaneously i ...	2007	17617827
[reaction of microorganisms to the digestive fluid of the earthworms].	the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ...	2007	17410875
evolution of catabolic pathways: genomic insights into microbial s-triazine metabolism.		2007	17114259
metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake.	in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ...	2007	17406774
n-ethylmaleimide-resistant acyl-coenzyme a oxidase from arthrobacter ureafaciens nbrc 12140: molecular cloning, gene expression and characterization of the recombinant enzyme.	n-ethylmaleimide (nem)-resistant acyl-coenzyme a oxidase (aco) has been desired for the determination of free fatty acids (ffas). in order to meet this demand, we prepared recombinant aco from arthrobacter ureafaciens nbrc 12140. the coding region of the gene was 2109, encoding a protein of 703 amino acids with a predicted molecular mass of 76.5 kda. the heterologous expression level in escherichia coli was 520-fold higher than that in the native strain. the purified enzyme retained more than 60 ...	2007	17141592
the coda transgene for glycinebetaine synthesis increases the size of flowers and fruits in tomato.	the tolerance of various species of plant to abiotic stress has been enhanced by genetic engineering with certain genes. however, the use of such transgenes is often associated with negative effects on growth and productivity under non-stress conditions. the coda gene from arthrobacter globiformis is of particular interest with respect to the engineering of desirable productive traits in crop plants. the expression of this gene in tomato plants resulted in significantly enlarged flowers and frui ...	2007	17362485
growth kinetics of microorganisms isolated from alaskan soil and permafrost in solid media frozen down to -35 degrees c.	we developed a procedure to culture microorganisms below freezing point on solid media (cellulose powder or plastic film) with ethanol as the sole carbon source without using artificial antifreezes. enrichment from soil and permafrost obtained on such frozen solid media contained mainly fungi, and further purification resulted in isolation of basidiomycetous yeasts of the genera mrakia and leucosporidium as well as ascomycetous fungi of the genus geomyces. contrary to solid frozen media, the enr ...	2007	17026514
biodiversity of bacterial ecosystems in traditional egyptian domiati cheese.	bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ...	2007	17189434
isolation and characterization of novel atrazine-degrading microorganisms from an agricultural soil.	six previously undescribed microorganisms capable of atrazine degradation were isolated from an agricultural soil that received repeated exposures of the commonly used herbicides atrazine and acetochlor. these isolates are all gram-positive and group with microorganisms in the genera nocardioides and arthrobacter, both of which contain previously described atrazine degraders. all six isolates were capable of utilizing atrazine as a sole nitrogen source when provided with glucose as a separate ca ...	2007	17318536
molecular identification and differentiation of brevibacterium species and strains.	amplified ribosomal dna restriction enzyme analysis (ardra), pulsed field gel electrophoresis (pfge) and ribotyping were used to differentiate among 24 strains of brevibacterium linens, brevibacterium casei and brevibacterium epidermidis obtained from type culture collections or isolated from various smear ripened cheeses. ardra was applied to the 16s rdna. b. linens was shown to be a quite heterogenic group with 2 to at least 4 copies of rrn operons per strain with aberrant nucleotide sequences ...	2007	16624515
biotransformation of isoeugenol to vanillin by pseudomonas putida ie27 cells.	the ability to produce vanillin and/or vanillic acid from isoeugenol was screened using resting cells of various bacteria. the vanillin- and/or vanillic-acid-producing activities were observed in strains belonging to the genera achromobacter, aeromonas, agrobacerium, alcaligenes, arthrobacter, bacillus, micrococcus, pseudomonas, rhodobacter, and rhodococcus. strain ie27, a soil isolate showing the highest vanillin-producing activity, was identified as pseudomonas putida. we optimized the culture ...	2007	16944125
application of thermoalkalophilic xylanase from arthrobacter sp. mtcc 5214 in biobleaching of kraft pulp.	a thermoalkalophilic and cellulase-free xylanase produced from arthrobacter sp. mtcc 5214 by solid-state fermentation using wheat bran as a carbon source was evaluated for prebleaching of kraft pulp. the uv absorption spectrum of the compounds released by enzyme treatment showed a characteristic peak at 280 nm, indicating the presence of lignin in the released colouring matter. enzymatic prebleaching of kraft pulp showed 20% reduction in kappa number of the pulp without much change in viscosity. ...	2007	16617015
characterization of bacterial isolates from industrial wastewater according to probable modes of hexadecane uptake.	bacterial isolates from industrial wastewater were characterized according to probable modes of hexadecane uptake based on data for cell surface hydrophobicity, emulsifying activity, glycoside content and surface tension of cell-free culture medium. the results obtained suggested that both modes of biosurfactant-enhanced hexadecane uptake by bacterial strains take place, direct uptake and alkane transfer. the increase in cell surface hydrophobicity and glycoside production by the strains suggest ...	2008	16962302
biodegradation and bioremediation of endosulfan contaminated soil.	among the three mixed bacterial culture ae, be, and ce, developed by enrichment technique with endosulfan as sole carbon source, consortium ce was found to be the most efficient with 72% and 87% degradation of alpha-endosulfan and beta-endosulfan, respectively, in 20 days. in soil microcosm, consortium ae, be and ce degraded alpha-endosulfan by 57%, 88% and 91%, respectively, whereas beta-endosulfan was degraded by 4%, 60% and 67% after 30 days. ochrobacterum sp., arthrobacter sp., and burkholde ...	2008	17646098
structure and denaturation of 4-chlorobenzoyl coenzyme a dehalogenase from arthrobacter sp. strain tm-1.	the secondary structure of the trimeric protein 4-chlorobenzoyl coenzyme a dehalogenase from arthrobacter sp. strain tm-1, the second of three enzymes involved in the dechlorination of 4-chlorobenzoate to form 4-hydroxybenzoate, has been examined. e(mm) for the enzyme was 12.59. analysis by circular dichroism spectrometry in the far uv indicated that 4-chlorobenzoyl coenzyme a dehalogenase was composed mostly of alpha-helix (56%) with lesser amounts of random coil (21%), beta-turn (13%) and beta ...	2008	17431803
biosorption of cr (vi) using a bacterial biofilm supported on granular activated carbon and on zeolite.	two mini-columns partially filled with granular activated carbon (gac) and/or a natural zeolite, covered by a bacterial biofilm of arthrobacter viscosus, were used in a continuous flow system to remove cr (vi) from solutions with initial concentration of 70mg/l and a working ph ranging between 4.5 and 5.5. three different set-up's were used: two columns in series filled gac covered with a biofilm, two columns in series filled with zeolite covered with a biofilm and a column filled with gac follo ...	2008	17368891
synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-beta-n-acetylglucosaminidases.	a facile synthesis of oligosaccharide-thiazoline derivatives of n-glycans as a novel class of inhibitors for endo-beta-n-acetylglucosaminidases was described. it was found that the external sugar residues on the n-glycan core could enhance the inhibitory potency. while the manbeta1,4glcnac- and man3glcnac-thiazolines were only moderate inhibitors, the large man9glcnac-thiazoline demonstrated potent inhibitory activity, with an ic(50) of 0.22 and 0.42 microm against the arthrobacter enzyme (endo- ...	2008	18304822
characterization of a cryptic plasmid from a greenland ice core arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high g+c gram-positive recipients.	over 60 greenland glacial isolates were screened for plasmids and antibiotic resistance/sensitivity as the first step in establishing a genetic system. sequence analysis of a small, cryptic, 1,950 bp plasmid, p54, from isolate gic54, related to arthrobacter agilis, showed a region similar to that found in theta replicating rhodococcus plasmids. a 6,002 bp shuttle vector, psvj21, was constructed by ligating p54 and puc18 and inserting a chloramphenicol acetyl transferase (cat) cassette conferring ...	2008	18335166
arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment.	a novel gram-positive bacterium, designated syb2t, was isolated from wastewater reservoir sediment, and a polyphasic taxonomic study was conducted based on its morphological, physiological, and biochemical features, as well as the analysis of its 16s rrna gene sequence. during the phylogenetic analysis of the strain syb2t, results of a 16s rrna gene sequence analysis placed this bacterium in the genus arthrobacter within the family micrococcaceae. syb2t and arthrobacter protophormiae atcc 19271t ...	2008	18337691
commercial ripening starter microorganisms inoculated into cheese milk do not successfully establish themselves in the resident microbial ripening consortia of a south german red smear cheese.	production of smear-ripened cheese critically depends on the surface growth of multispecies microbial consortia comprising bacteria and yeasts. these microorganisms often originate from the cheese-making facility and, over many years, have developed into rather stable, dairy-specific associations. while commercial smear starters are frequently used, it is unclear to what degree these are able to establish successfully within the resident microbial consortia. thus, the fate of the smear starters ...	2008	18281427
rapid mineralization of the phenylurea herbicide diuron by variovorax sp. strain srs16 in pure culture and within a two-member consortium.	the phenylurea herbicide diuron [n-(3,4-dichlorophenyl)-n,n-dimethylurea] is widely used in a broad range of herbicide formulations, and consequently, it is frequently detected as a major water contaminant in areas where there is extensive use. we constructed a linuron [n-(3,4-dichlorophenyl)-n-methoxy-n-methylurea]- and diuron-mineralizing two-member consortium by combining the cooperative degradation capacities of the diuron-degrading organism arthrobacter globiformis strain d47 and the linuro ...	2008	18296530
biofiltration of trimethylamine, dimethylamine, and methylamine by immobilized paracoccus sp. cp2 and arthrobacter sp. cp1.	a biofilter using granular activated carbon with immobilized paracoccus sp. cp2 was applied to the elimination of 10-250 ppm of trimethylamine (tma), dimethylamine (dma), and methylamine (ma). the results indicated that the system effectively treated ma (>93%), dma (>90%), and tma (>85%) under high loading conditions, and the maximum degradation rates were 1.4, 1.2, and 0.9g-nkg(-1) gac d(-1). among the three different amines treated, tma was the most difficult to degrade and resulted in ammonia ...	2008	18331754
functional diversity and electron donor dependence of microbial populations capable of u(vi) reduction in radionuclide-contaminated subsurface sediments.	in order to elucidate the potential mechanisms of u(vi) reduction for the optimization of bioremediation strategies, the structure-function relationships of microbial communities were investigated in microcosms of subsurface materials cocontaminated with radionuclides and nitrate. a polyphasic approach was used to assess the functional diversity of microbial populations likely to catalyze electron flow under conditions proposed for in situ uranium bioremediation. the addition of ethanol and gluc ...	2008	18378664
chemostat selection of a bacterial community able to degrade s-triazinic compounds: continuous simazine biodegradation in a multi-stage packed bed biofilm reactor.	using a successive transfer method on mineral salt medium containing simazine, a microbial community enriched with microorganisms able to grow on simazine was obtained. afterwards, using a continuous enrichment culture procedure, a bacterial community able to degrade simazine from an herbicide formulation was isolated from a chemostat. the continuous selector, fed with a mineral salt medium containing simazine and adjuvants present in the commercial herbicide formulation, was maintained in opera ...	2008	18392868
[isolation of antibiotic resistance bacterial strains from east siberia permafrost sediments].	a collection of bacterial antibiotic resistance strains isolated from arctic permafrost subsoil sediments of various age and genesis was created. the collection included approximately 100 strains of gram-positive (firmicutes, arthrobacter) and gram-negative bacteria (bacteroidetes, gamma-proteobacteria, and alpha-proteobacteria) resistant to aminoglycoside antibiotics (gentamycin, kanamycin, and streptomycin), chloramphenicol and tetracycline. antibiotic resistance spectra were shown to differ i ...	2008	18409385
genetic analysis of phenylacetic acid catabolism in arthrobacter oxydans cect386.	arthrobacter oxydans cect386 is a gram-positive bacterium able to use either phenylacetic acid or phenylacetaldehyde as the sole carbon and energy source for aerobic growth. genes responsible for the catabolism of these compounds have been located at two chromosomal regions and were organized in one isolated paan gene and two putative paa operons, one consisting of the paad, paaf, tetr and prot genes, and one consisting of the paag, paah, paai, paaj, paak and paab genes. the identity of the paaf ...	2008	18437357
structure of 2,6-dihydroxypyridine 3-hydroxylase from a nicotine-degrading pathway.	the enzyme 2,6-dihydroxypyridine-3-hydroxylase catalyzes the sixth step of the nicotine degradation pathway in arthrobacter nicotinovorans. the enzyme was produced in escherichia coli, purified and crystallized. the crystal structure was solved at 2.6 a resolution, revealing a significant structural relationship with the family of fad-dependent aromatic hydroxylases, but essentially no sequence homology. the structure was aligned with those of the established family members, showing that the fad ...	2008	18440023
study of cadmium, zinc and lead biosorption by orange wastes using the subsequent addition method.	the biosorption of several metals (cd2+, zn2+ and pb2+) by orange wastes has been investigated in binary systems. multicomponent sorption isotherms were obtained using an original procedure, similar to that proposed by pagnanelli et al. [pagnanelli, f., petrangeli, m.p., toro, l., trifoni, m., veglio, f., 2001a. biosorption of metal ions on arthrobacter sp.: biomass characterization and biosorption modelling. environ. sci. technol. 34, 2773-2778] for monoelement systems, known as subsequent addi ...	2008	18440805
[restriction endonuclease asi256i recognizing the nucleotide sequence 5'-gatc-3'].	a strain producing a restriction endonuclease was isolated from soil samples and identified as the arthrobacter sp. strain ck256. the enzyme produced by this strain was termed asi2561. the isolation procedure for this enzyme was described, and the optimal conditions for its function were determined. it was shown that the restriction endonuclease asi256i is a true isoschizomer of mboi, it has a temperature optimum of 6 degrees c, and can be used in molecular-biological and genetic-engineering stu ...	2008	18491594
swapping metals in fe- and mn-dependent dioxygenases: evidence for oxygen activation without a change in metal redox state.	biological o(2) activation often occurs after binding to a reduced metal [e.g., m(ii)] in an enzyme active site. subsequent m(ii)-to-o(2) electron transfer results in a reactive m(iii)-superoxo species. for the extradiol aromatic ring-cleaving dioxygenases, we have proposed a different model where an electron is transferred from substrate to o(2) via the m(ii) center to which they are both bound, thereby obviating the need for an integral change in metal redox state. this model is tested by usin ...	2008	18492808
enantiomer-specific binding of ruthenium(ii) molecular wires by the amine oxidase of arthrobacter globiformis.	the copper amine oxidase from arthrobacter globiformis (agao) is reversibly inhibited by molecular wires comprising a ru(ii) complex head group and an aromatic tail group joined by an alkane linker. the crystal structures of a series of ru(ii)-wire-agao complexes differing with respect to the length of the alkane linker have been determined. all wires lie in the agao active-site channel, with their aromatic tail group in contact with the trihydroxyphenylalanine quinone (tpq) cofactor of the enzy ...	2008	18507382
the shortest wire.		2008	18508968
catalytic mechanism of inulinase from arthrobacter sp. s37.	detailed catalytic roles of the conserved glu323, asp460, and glu519 of arthrobacter sp. s37 inulinase (enia), a member of the glycoside hydrolase family 32, were investigated by site-directed mutagenesis and ph-dependence studies of the enzyme efficiency and homology modeling were carried out for enia and for d460e mutant. the enzyme efficiency (k(cat)/k(m)) of the e323a and e519a mutants was significantly lower than that of the wild-type due to a substantial decrease in k(cat), but not due to ...	2008	18395004
arthrobacter oryzae sp. nov. and arthrobacter humicola sp. nov.	two novel bacterial strains were isolated from a paddy soil sample collected in japan using gpm agar plates supplemented with superoxide dismutase and/or catalase. the strains were gram-positive, catalase-positive and motile, with lysine as the peptidoglycan diagnostic diamino acid and acetyl as the peptidoglycan acyl type. the major menaquinone was mk-9(h(2)). mycolic acids were not detected. the g+c content of the dna was 66-68 mol%. on the basis of phenotypic analysis, 16s rrna gene sequence ...	2008	18175682
degradation of 4-fluorophenol by arthrobacter sp. strain if1.	a gram-positive bacterial strain capable of aerobic biodegradation of 4-fluorophenol (4-fp) as the sole source of carbon and energy was isolated by selective enrichment from soil samples collected near an industrial site. the organism, designated strain if1, was identified as a member of the genus arthrobacter on the basis of 16s ribosomal rna gene sequence analysis. arthrobacter strain if1 was able to mineralize 4-fp up to concentrations of 5 mm in batch culture. stoichiometric release of fluor ...	2008	18228015
novel chromium tolerant microorganisms: isolation, characterization and their biosorption capacity.	effluent from tannery, electroplating and electronic industries contain chromium which is highly toxic. this paper reports isolation of chromium tolerant microorganisms from solid waste as well as liquid effluent of an electroplating industry. nine isolates were obtained that can tolerate chromium concentration up to 700mg/l. they reach their stationary phase within 8-12h and can biosorb 95% of initial 200mg/l concentration of chromium within 4-10h. fourier transform infra red analysis of the bi ...	2008	18272220
effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation.	spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ...	2008	18083857
oligosaccharide profiles of the prostate specific antigen in free and complexed forms from the prostate cancer patient serum and in seminal plasma: a glycopeptide approach.	the oligosaccharide structures of prostate specific antigen (psa) are expected to be useful in discriminating prostate cancer from benign conditions both accompanied by increased serum psa levels. a large proportion of psa forms a covalent complex with a glycoprotein, alpha(1)-antichymotrypsin, in human blood. in the present study, the glycan profiles of free and complexed forms of psa from cancer patient serum and of seminal plasma psa were compared by analyzing the glycopeptides obtained by ly ...	2008	17956937
chromate reduction by arthrobacter cr47 in biofilm packed bed reactors.	bacterial strain cr47 was isolated from a landfarming process soil sample. it was identified, by 16s rdna sequencing, as arthrobacter sp. the time course of the cr(vi) reduction was monitored in batch operated packed bed biofilm reactors (12 ml void volume) and in recirculating packed bed biofilm reactors (100 ml void volume) inoculated with bacterial strain cr47. the reduction was evaluated with, 30 mg l(-1) cr(vi) laboratory solutions prepared with k2cr2o7 and enriched with glucose-minimal med ...	2008	18063473
two closely related pathways of nicotine catabolism in arthrobacter nicotinovorans and nocardioides sp. strain js614.	a virtually identical nicotine catabolic pathway including the heterotrimeric molybdenum enzyme nicotine and 6-hydroxy-pseudo-oxynicotine dehydrogenase, 6-hydroxy-l: -nicotine oxidase, 2,6-dihydroxy-pseudo-oxynicotine hydrolase, and 2,6-dihydroxypyridine hydroxylase have been identified in a. nicotinovorans and nocardioides sp. js614. enzymes catalyzing the same reactions and similar protein antigens were detected in the extracts of the two microorganisms. nicotine blue and methylamine, two end ...	2008	18071673
effect of immobilization on kinetic and thermodynamic characteristics of sulfide oxidase from arthrobacter species.	in order to determine the impact of immobilization on biocatalytic efficacy of sulfide oxidase, the kinetic and thermodynamic properties of native and deae-cellulose immobilized sulfide oxidase from arthrobacter species fr-3 were evaluated. immobilization increased the catalytic efficiency of sulfide oxidase by producing a lower michaelis-menten constant (km) and a higher rate of catalysis (vmax) at different temperatures. the first-order kinetic analysis of thermal denaturation demonstrated tha ...	2008	18080911
comparative analysis of eight arthrobacter plasmids.	despite the prevalence of arthrobacter in the environment little is known about their plasmids, or the capacity of arthrobacter plasmids to mediate horizontal gene transfer. in this study, we compared eight plasmids from five arthrobacter strains in order to identify putative core maintenance genes for replication, segregation, and conjugation. iteron like sequences were identified on some of the plasmids; however, no genes with obvious similarity to known replication sequences such as an origin ...	2008	18241920
the effect of cysteine on production of volatile sulphur compounds by cheese-ripening bacteria.	the effect of cysteine on the ability of smear cheese-ripening bacteria (brevibacterium linens and arthrobacter spp) to produce volatile sulphur compounds (vsc) from methionine was studied. these bacteria were cultivated in a synthetic medium supplemented with various cysteine concentrations with or without methionine. cultures with only cysteine showed slightly lower levels of vsc produced and an unpleasant odour like rotten eggs, resulting from hydrogen sulphide production. the levels and prof ...	2008	18262300
complete degradation of butyl benzyl phthalate by a defined bacterial consortium: role of individual isolates in the assimilation pathway.	two bacterial strains, in consortium, were isolated by enrichment techniques from municipal waste-contaminated soil, which utilized butyl benzyl phthalate (bbp) as the sole carbon source. one of the isolates was identified as arthrobacter sp. strain wy and the other one as acinetobacter sp. strain fw based on the morphological, nutritional and biochemical characteristics and 16s rrna sequence analysis. various metabolites of bbp engendered by arthrobacter sp. strain wy were isolated and identifi ...	2008	17669462
trans-stilbene degradation by arthrobacter sp. sl3 cells.	trans-stilbene degradation was examined by the reaction using resting cells of microorganisms isolated through the enrichment culture using trans-stilbene. the strain sl3, showing the highest trans-stilbene-degrading activity, was identified as arthrobacter sp. one of the reaction products was identified to be cis,cis-muconic acid. arthrobacter sp. sl3 cells also transformed benzaldehyde, benzoic acid and catechol into cis,cis-muconic acid, suggesting that one benzene ring of trans-stilbene was ...	2008	17885812
degradation of mixtures of phenolic compounds by arthrobacter chlorophenolicus a6.	in this study the chlorophenol-degrading actinobacterium, arthrobacter chlorophenolicus a6, was tested for its ability to grow on mixtures of phenolic compounds. during the experiments depletion of the compounds was monitored, as were cell growth and activity. activity assays were based on bioluminescence output from a luciferase-tagged strain. when the cells were grown on a mixture of 4-chlorophenol, 4-nitrophenol and phenol, 4-chlorophenol degradation apparently was delayed until 4-nitrophenol ...	2008	17917705
cultivable bacterial diversity of alkaline lonar lake, india.	aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season, january 2002 from alkaline lonar lake, india, having ph 10.5. the total number of microorganisms in the sediment and water samples was found to be 10(2)-10(6) cfu g(-1) and 10(2)-10(4) cfu ml(-1), respectively. one hundred and ninety-six strains were isolated using different enrichment media. to study the bacterial diversity of lonar lake and to select the bacterial stra ...	2008	17604989
the distribution of acetohydroxyacid synthase in soil bacteria.	most bacteria possess the enzyme acetohydroxyacid synthase, which is used to produce branched-chain amino acids. enteric bacteria contain several isozymes suited to different conditions, but the distribution of acetohydroxyacid synthase in soil bacteria is largely unknown. growth experiments confirmed that escherichia coli, salmonella enterica serotype typhimurium, and enterobacter aerogenes contain isozymes of acetohydroxyacid synthase, allowing the bacteria to grow in the presence of valine (w ...	2008	17624809
simultaneous mineralization of glyphosate and diuron by a consortium of three bacteria as free- and/or immobilized-cells formulations.	a bacterial consortium able to mineralize two herbicides, glyphosate (pseudomonas 4asw) and diuron (arthrobacter sp. n4 and delftia acidovorans), was cultivated in both a synthetic culture medium without phosphate and a sediment extract medium. in the aim at optimizing glyphosate and diuron mineralization, all the combinations, i.e., free and/or immobilized cells in ca-alginate beads were tested. with the synthetic medium, the simultaneous mineralization of glyphosate and diuron required at leas ...	2008	18026726
application of pcr-sscp for molecular epidemiological studies on the exposure of farm children to bacteria in environmental dust.	the environmental exposure of farm children to microorganisms in dust has become a focus of interest, since microbial exposure on farms has been related to a reduced prevalence of asthma and atopic diseases in children. previous studies almost exclusively focused on the determination of microbial counts using conventional culturing or the determination of microbial compounds i.e. endotoxins. in this study pcr-sscp (single-strand conformation polymorphism) was modified for characterising bacteria ...	2008	18321601