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whipple's disease, familial mediterranean fever, adult-onset still's disease, and enteropathic arthritis.whipple's disease is a rare multisystem disorder of infectious etiology. efforts to culture the responsible organism have been unsuccessful. nucleotide sequencing and amplification of bacterial 16s ribosomal dna revealed the organism to be most similar to bacteria of the rhodococcus, streptomyces, and arthrobacter genera. several clinical studies of the long-term use of colchicine for the treatment of familial mediterranean fever demonstrate its utility for symptom control and prevention of comp ...19921380277
inhibition of development of kaposi's sarcoma-related lesions by a bacterial cell wall complex.in vitro and in vivo model systems for the study of human immunodeficiency virus (hiv)-associated kaposi's sarcoma (ks) were used to evaluate compounds for their potential as therapeutic agents. a sulfated polysaccharide-peptidoglycan compound (sp-pg) produced by bacteria controlled the in vitro growth of acquired immunodeficiency syndrome (aids)-associated, ks-derived spindle-shaped cells (aids-ks cells) at noncytotoxic concentrations. angiogenesis induced by aids-ks cells in the chicken chorio ...19921371891
screening of whole-cell immobilization procedures for the delta 1-dehydrogenation of steroids in organic medium.different whole-cell immobilization methods and conditions were tested for the delta 1-dehydrogenation of 6-alpha-methyl-hydrocortisone-21-acetate with arthrobacter simplex cells, in n-decane-1-ol and chloroform. among the entrapment methods, polyurethane foams gave the best productivity of n-decane-1-ol, but could not be used in chloroform. partition and diffusional barriers appeared to be the factors limiting productivity in entrapped-cell systems. the coentrapment of enzyme-stabilizing (glyce ...19921368890
purification and some properties of beta-fructofuranosidase i from arthrobacter sp. k-1.arthrobacter sp. k-1, isolated from soil, produces beta-fructofuranosidase. this enzyme preparation was separated into three fractions (i, ii, and iii) by butyl-toyopearl 650 m column chromatography. the beta-fructofuranosidase i was purified to homogeneity by disc-electrophoresis after consecutive column chromatographies. the enzyme had a molecular weight of 52,000 by sds-polyacrylamide gel electrophoresis and 51,000 by gel filtration with ultrogel aca 44, and an isoelectric point of 4.3. the e ...19901368550
action of levan fructotransferase of arthrobacter ureafaciens on three oligosaccharides containing a bifurcose residue. 19901368538
evidence for two distinct phosphonate-degrading enzymes (c-p lyases) in arthrobacter sp. glp-1.arthrobacter sp. glp-1 can utilize a wide range of organophosphonates as its sole source of phosphorus. the in-situ formation of sarcosine and methane from glyphosate and methanephosphonic acid respectively was studied. these two processes are differentially induced during phosphorus-deprivation. methanephosphonic acid strongly inhibits glyphosate degradation (i50 10 microm), but glyphosate has very little effect on methane generation (i50 150 mm). the pattern of inhibition by other organophosph ...19911368477
action of levan fructotransferase of arthrobacter ureafaciens on a mixture of branched levanpentasaccharides. 19921368345
structural study of cell wall mannan of a candida albicans (serotype a) strain.the structure of the mannan of candida albicans nih a-207 strain (serotype a) was investigated by adopting mild acetolysis followed by enzymolysis with an arthrobacter gjm-1 exo-alpha-mannosidase. the resultant oligosaccharides, from pentaose to octaose (where manp = d-mannopyranose), were identified as manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp, manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)- manp alpha (1----2)manp, manp beta (1- ...19921368047
stereospecific microbial reduction of 4,5-dihydro-4-(4-methoxyphenyl)-6-(trifluoromethyl-1h-1)-benzazepin+ ++-2-o ne.a key intermediate, (3r-cis)-1,3,4,5-tetrahydro-3-hydroxy-4-(4-methoxyphenyl)-6-(trifluorome thyl)- 2h-1-benzazepin-2-one (compound ii or sq32191), with high optical purity was made by the stereoselective microbial reduction of the parent ketone 1. several strains of bacterial and yeast cultures were screened for the ability to catalyse the stereoselective reduction of 4,5-dihydro-4-(4-methoxyphenyl)-6-(trifluoromethyl)-1h-1-benzazepin++ +-2,3-dione [compound i or sq32425]. microorganisms from t ...19911368001
continuous delta 1-hydrocortisone dehydrogenation with in situ product recovery.a continuous aerated process for delta 1-hydrocortisone dehydrogenation by polyacrylamide-hydrazide (paah) bead-entrapped a. simplex cells was developed. the process allows for stable conversion of 1.6 g l-1 hydrocortisone (x 5 the solubility in water), made possible by the incorporation of selected cosolvent [5% (v/v) triethyleneglycol]. a large difference in substrate and product solubilities in the cosolvent-buffer medium allowed for in situ product recovery in an aerated, fluidized-bed, immo ...19911367997
immobilization of arthrobacter simplex in thermally reversible hydrogels: effect of gel hydrophobicity on steroid conversion.arthrobacter simplex cells have been immobilized in a series of thermally reversible hydrogels having different gel hydrophobicities. steroid conversion from hydrocortisone to prednisolone via the delta 1-dehydrogenase system was greatly affected by the relative hydrophobicities of the gel matrices, which were prepared by copolymerizing varying ratios of n-isopropylacrylamide to acrylamide. the characteristics of the immobilized cells, such as optimal temperatures, km values, and the effects of ...19911367990
delta'-dehydrogenation of steroids by arthrobacter simplex immobilized in calcium polygalacturonate beads.arthrobacter simplex atcc 6946 (viable cells) was immobilized in a calcium polygalacturonate gel. the trapped cells were used for repeated batchwise bioconversion of steroids. reichstein's compound s and hydrocortisone were dehydrogenated introducing a double bond between c1 and c2 of ring a. the products 1-dehydro s and prednisolone, respectively, were identified by high pressure liquid chromatography. steroid dehydrogenase activity increased in the system when an artificial electron acceptor, ...19911367800
production of l-tryptophan from d,l-5-indolylmethylhydantoin by resting cells of a mutant of arthrobacter species (dsm 3747).the reaction parameters and the stereospecificity of the enzymatic cleavage of d,l-5-indolylmethylhydantoin in producing l-tryptophan with resting cells of arthrobacter sp. dsm 3747 were studied. when intact cells were tested, the optimal ph was between 8.5 and 9.0 and the optimal temperature was 50 degrees c. both, l-n-carbamoylase and hydantoinase could be stabilized over 24 h at 30 and 40 degrees c by the addition of d,l-5-indolylmethylhydantoin. furthermore, the hydantoinase was stable over ...19901366911
cell growth and enzyme synthesis of a mutant of arthrobacter sp. (dsm 3747) used for the production of l-amino acids from d,l-5-monosubstituted hydantoins.a microorganism with the ability to form l-tryptophan from d,l-5-(3-indolyl-methyl)hydantoin (d,l-5-imh) was isolated and identified as arthrobacter sp. (dsm 3747). after isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high amounts of biomass with good specific activities concerning the enzymatic hydantoin-cleaving reactions. the ability of the microorganism to perform these bioconversions was found to b ...19901366910
nadh production from nad+ with a formate dehydrogenase system involving immobilized cells of a methylotrophic arthrobacter strain.a convenient and economical method of nadh production from nad+ has been established using a formate dehydrogenase system involving immobilized cells of a methanol-utilizing bacterium. arthrobacter sp, km62. four kinds of cell entrapment were studied. an immobilized cell preparation showing a high nadh production activity was obtained by entrapment in a kappa-carrageenan gel lattice. the nadh-producing activity of the immobilized cells was investigated under various conditions. the nadh-producin ...19901366576
sites of cadmium uptake in bacteria used for biosorption.electron microscopy and x-ray spectroscopy were used to determine location and type of cadmium biosorption on and in bacteria, some of which produced extracellular polymers. examined arthrobacter and pseudomonas species appear to have detoxification systems that precipitate cadmium internally irrespective of whether or not they excrete polymers. capsular klebsiella aerogenes strains showed minimal intracellular uptake but over a 5-100 mg dm-3 cd range produced the highest net metal removal level ...19901366533
transformation of microcrystalline hydrocortisone by free and immobilized cells of arthrobacter simplex.the transformation of microcrystalline hydrocortisone by free and immobilized cells of arthrobacter simplex resulted in formation of prednisolone. the effect of medium composition, non-ionogenic surfactants and exogenous electron acceptors on the delta 1-dehydrogenase activity of free and immobilized cells is described.19901366456
the use of free and immobilised arthrobacter simplex in organic solvent/aqueous two-liquid-phase reactors.the use of free and immobilised arthrobacter simplex (ncib 8929) for steroid delta 1-dehydrogenation in two-liquid-phase, stirred-tank reactors has been compared. product formation is related to the logarithm of the water-octanol partition coefficient (log p) of the organic solvent employed, but the relationship is different for the two forms of the biocatalyst. no reaction was seen with either biocatalyst in media containing solvents of log p less than or equal to 2.5. for free bacteria, produc ...19901366455
trimethyl lead degradation by free and immobilized cells of an arthrobacter sp. and by the wood decay fungus phaeolus schweinitzii.the continuing production of leaded petrol generates liquid wastes containing recalcitrant trialkyl lead, for which no suitable chemical treatment has been formulated. this investigation explores the feasibility of using microorganisms to catalyse the rate-limiting step of trimethyl lead degradation to dialkyl lead; this disproportionates chemically to give, ultimately, pb2+ which is treatable by classical methods. an arthrobacter sp. and a wood decay macrofungus, phaeolus schweinitzii provide n ...19901366364
acsi, a new restriction endonuclease from arthrobacter citreus 310 recognizing 5'-pu decreases aattpy-3'. 19921322532
effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes.tetralin is toxic to bacterial cells at concentrations below 100 mumol/liter. to assess the inhibitory action of tetralin on bacterial membranes, a membrane model system, consisting of proteoliposomes in which beef heart cytochrome c oxidase was reconstituted as the proton motive force-generating mechanism, and several gram-positive and gram-negative bacteria were studied. because of its hydrophobicity, tetralin partitioned into lipid membranes preferentially (lipid/buffer partition coefficient ...19921314806
[cloning and expression of the arthrobacter globiformis fcb genes in bacillus subtilis].the fcb genes of arthrobacter globiformis kzt1 coding for the dehalogenase (4-chlorobenzoate-4-hydroxylase) activity have been cloned. the characteristics of fcb genes expression have been studied. the recombinant strains of bacillus subtilis 6jm15 (pcbs 311) and 6jm15 (pcbs1) have shown the decreased level of substrate dehalogenation as compared with the one in the parent strain kzt1 and the recombinant strains of escherichia coli and pseudomonas putida.19921301499
multi-recognition capability of e-selectin in a dynamic flow system, as evidenced by differential effects of sialidases and anti-carbohydrate antibodies on selectin-mediated cell adhesion at low vs. high wall shear stress: a preliminary note.e-selectin has a "multi-recognition" capability in terms of epitope binding specificity, depending on adhesion conditions (static vs. low- or high-shear stress dynamic systems). specifically, (i) adhesion based on expression of alpha 2-->3 sialylated le(x) (sle(x)) is prominent under static or low shear stress dynamic conditions; (ii) adhesion under high shear stress dynamic conditions does not depend on the known sle(x) species, but rather on lex with an adjacent unidentified sialosyl substitut ...19921282810
effects of temperature on the growth of psychrophilic bacteria from glaciers.growth of five strains of psychrophilic bacteria (four arthrobacter and one pseudomonas) isolated from glacial deposits was studied at different temperatures. three strains were facultative psychrophiles, having an optimum temperature for growth at about 25-28 degrees c and a maximum at about 32-34 degrees c. the two arthrobacter glacialis strains were found to be obligate psychrophiles with an optimum at 13-15 degrees c and a maximum at 18 degrees c. arrhenius plots showed that a. glacialis cou ...19761277005
a specific endonuclease from arthrobacter luteus. 19761271462
colonization of soil by arthrobacter and pseudomonas under varying conditions of water and nutrient availability as studied by plate counts and transmission electron microscopy.arthrobacter globiformis and a pseudomonas soil isolate were incubated separately and in combination in soil that had been presterilized by autoclaving. growth and other responses of the cells in situ in this soil were monitored by plate counts and transmission electron microscopy examinations of cell sections. during the soil incubations, some of the samples were first allowed to dry and then were remoistened with water or with a dilute or a concentrated nutrient solution. based on plate counts ...19761267449
mechanism of reversible glycine cleavage reaction in arthrobacter globiformis. function of lipoic acid in the cleavage and synthesis of blycine. 19761259444
enzymic hydrolysis of di-d-fructofuranose 1, 2'; 2, 3' dianhydride with arthrobacter ureafaciens.enzymic hydrolysis of di-d-fructofuranose 1, 2'; 2, 3' dianhydride with the bacteria arthrobacter ureafaciens was studied to elucidate its mechanism. hydrolysis of the difructose dianhydride to d-fructose, which did not occur with yeast invertase [ec 3.2.1.26], was found to occur on incubation with an enzyme preparation from an autolysate of the above bacteria. however, incubation with enzyme which had been treated at 60 degrees for 30 min yielded an intermediate hydrolysis product. the product ...19751225919
amino acid composition and physiochemical characterization of chondroitinase from arthrobacter aurescens.the amino acid and carbohydrate compositions of chondroitinase ac [ec 4.2.2.5] from arthrobacter aurescens were determined, and its physicochemical properties were examined. 1. the enzyme has been shown to be a glycoprotein containing mannose, glucose, glucosamine, and glucuronic acid (3:5:4:2). 2. its molecular wieght was estimated to be 76,000 by gel filtration on sephadex g-200, 75,000-80,000 by sds disc electrophoresis, and 75,800 by sedimentation veolcity. no subunits were detected in the m ...19751225917
microbial metabolism of 2-hydroxypyridine. 19751221039
pyrrolidine metabolism and its regulation in arthrobacter sp. 19751221030
[synthesis of group b vitamins by bacteria of the genus arthrobacter]. 19751219320
microbial decomposition of 2-ethylpyridine, 2,4-lutidine & 2,4,6-collidine. 19751218933
[facultative methylotroph belonging to the genus arthrobacter].a bacterial strain has been isolated and identified, on the basis of its morphological and physiologo-biochemical properties, as arthrobacter globiformis. the bacterium is a facultative methylotroph and grows not only on media with various organic compounds but also in the presence of methylated amines as a sole source of carbon, nitrogen, and energy. other c1-substrates were not utilized.19751214618
[spectrophotometric characteristics of the coccoid forms of actinomycetes].ir spectra of thw whole cells of the coccoid forms (mycococcus and micrococcus) isolated from lithophilous lichen were compared with ir spectra of the collection cultures of micrococcus and arthrobacter. generic spectral characteristics of mycococcus and micrococcus are presented. spectral heterogeneity within the genus arthrobacter complicates the diagnosis. the cultures of the mycococcus genus were divided into three groups according to their spectral characteristics. spectral scans of the stu ...19751214609
bacterial carotenoids, xlvi. c50-carotenoids, 14. c50-carotenoids from arthrobacter glacialis.from the psychrophilic bacterium arthrobacter glacialis have been isolated three c50-carotenoids with molecular formulae c50h72o2: the bicyclic decaprenoxanthin (1a, 7% of total carotenoids), the aliphatic bisanhydrobacterioruberin (2a, 10%) and the monocyclic a.g. 470 (3a, 83%). decaprenoxanthin (1) and bisanhydrobacterioruberin (2) were in all respects, including chiroptical properties, identical with known carotenoids. the constitution of the previously undescribed a.g. 470 (3a) followed from ...19751211003
[microbiological transformation and degradation of pesticides]. 19751206144
the microbial degradation of cyclohexane carboxylic acid. 19751202160
peptidoglycan compositions of a new strain of arthrobacter crystallopietes during sphere-rod morphogenesis.arthrobacter crystallopoieties atcc 15481 was used to isolate a new strain. designated arthrobacter crystallopoieties epsr-16, which had a mass doubling time in brain heart infusion broth and in glucose/salts/yeast extract medium of 30 min compared to 2.40 h for the parent strain in similar media. the growth rates for the new strain and for the parent were close to 12 h in glucose/salts medium. the new strain formed well-separated cocci and diplococci in glucose/salts medium, and upon nutrient s ...19751201285
[on the taxonomy and physiology of bacteria utilizing hydrocarbons in the sea (author's transl)].using mineral media with gas oil as sole carbon source, 191 bacterial strains were isolated from the costal area of marseille. these strains were attributed to achromobacter, alcaligenes, pseudomonas, acinetobacter and arthrobacter genera. amongst isolated strains there was a predominance of the alcaligenes-achromobacter group over others genera. growth and respiratory activity of 5 strains were studied on hexadecane and acetate. respiratory activity on hexadecane of 32 strains cultured on aceta ...19751190640
[formation of primary alcohols and palmitic acid in the microbiological oxidation of hexadecane].forty seven microbial strains oxidized hexadecane to form primary cetol and palmitic acid. the maximum quantitiy of the compounds was accumulated in the exponential phase of growing cultures (for 72 hours) and for 6 to 16 hours of incubation of resting cell suspensions. candida yeast were shown by the gas-liquid chromatography method to be the most active producers of cetol.19751187568
[modifications of the antigenic composition of arthrobacter simplex atcc 6946 in the presence of reichstein's substance s]. 19751186519
induction of morphogenesis in the genus arthrobacter.thirty-eight different compounds were used with 17 species of arthrobacter to determine their ability to support growth and to induce the morphogenic cycle. in most cases, when a compound supported growth, it also induced the rod phase of growth. however, in a few cases, a compound would support growth with cells remaining in the coccoidal phase throughout the growth cycle. arthrobacter crystallopoietes was unique in the most compounds that supported growth did not induce the rod phase of the mo ...19751184572
stereochemistry of delta 1,4 unsaturation in microbial transformation of cholesterol. 19751182921
[arthrobacter siderocapsulatus isolated from lake water].two microbial strains have been isolated from lake water. the strains oxidize ferrous compounds and manganese. by the structure of microcolonies and the character of deposited oxides of these metals, the strains are identical to the genus siderocapsa. however, according to their growth cycle and some morpho-physiological characteristics, they were included into the genus arthrobacter (corynebacteriaceae). since these microorganisms differ, by their cultural and morpho-physiological properties, f ...19751177780
microbial cleavage of the tomatidine spiroketal sidechain. 19751177446
metabolism of dichlobenil by microorganisms in the aquatic environment. 19751150978
microbial metabolism of phenolic amines: degradation of dl-synephrine by an unidentified arthrobacter.microorganisms capable of degrading dl-synephrine were isolated from soil of citrus gardens by enrichment culture, with dl-synephrine as the sole source of carbon and nitrogen. an organism which appears to be an arthrobacter, but which cannot be identified with any of the presently recognized species was predominant in these isolates. it was found to metabolize synephrine by a pathway involving p-hydroxyphenylacetaldehyde, p-hydroxyphenylacetic acid, and 3,4-dihydroxyphenylacetic acid as interme ...19751150621
action of arthrobacter ureafaciens inulinase ii on several oligofructans and bacterial levans.1. arthrobacter ureafaciens inulinase ii which converts inulin to di-d-fructofuranose 1,2' : 2,3' dianhydride (difructose anhydride iii) leaving a small amount of oligosaccharides, was investigated in order to characterize its mode of action. 2. after the enzymatic reaction on the glucose-terminated inulin molecules had been completed, the oligosaccharides left in the enzyme digest were isolated, and identified to be the fructose-glucose oligosaccharides; o-beta-d-fructofuranosyl-(2 leads to 1)- ...19751148257
differentiation of arthrobacter soil isolates and named strains from other bacteria by reations on dye-containing media.twenty-five gram-negative and 62 gram-positive bacterial cultures, of which 37 werearthrobacters, were tested, using a multipoint inoculation device, for responses on varying concentrations of 35 dyes. both selective (growth vs. no growth) and differential (dye absorption) responses were obtained. it was possible to differentiate the arthrobacters from the other gram-positive bacteria tested as well as to distinguish separately each arthrobacter-named strain. the results indicated that dyes have ...19751125859
the microbial metabolism of di-n-butyl phthalate and related dialkyl phthalates. 19751125463
[crystal-transformation of hydrocortisone effected by a culture of mycobacterium globiforme 193]. 19751117087
a nutritional and taxonomic survey of arthrobacter soil isolates.one hundred and sixty arthrobacter soil isolates, four groups of 40 from each of four soil types, and 17 arthrobacter named strains were characterized on the basis of 203 different nutritional tests performed on each culture. one hundred and twenty-one compounds were examined as sole sources of carbon, 44 compounds as sole sources of nitrogen, 22 carbohydrates for the production of acid, and 16 tests were done for tolerance to various dyes. the four groups of soil arthrobacters differed markedly ...19751116046
indicator media for microorganisms degrading chlorinated pesticides.a bromocresol purple liquid indicator medium and an eosin-methylene blue agar have been developed for the demonstration and isolation of microorganisms able to degrade the chlorinated herbicide, 2,4-dichlorophenoxyacetic acid (2,4-d). plates of the eosin-methylene blue agar indicate individual 2,4-d-degrading bacterial colonies. both indicator systems show the production of acid, presumably hydrochloric, during degradation of the 2,4-d in the media. concentrations of 2,4-d required to give an ac ...19751116035
oxidative degradation of squalene by arthrobacter species.an organism isolated from soil and identified as arthrobacter sp. was studied for its squalene degradation. the degradation product from squalene, which accumulated in the culture broth, was isolated and identified as trans-geranylacetone by mass spectrometry, gas chromatography, infrared spectrometry, and nuclear magnetic resonance spectrometry. addition of a high concentration of k2hpo4 to the culture medium resulted in accumulation of fairly large amounts of carboxylic acids in addition to ge ...19751115507
microbiological characteristics of dungeness crab (cancer magister).aerobic, heterotropic microorganisms of dungeness crab (cancer magister) were isolated from raw crab, cooked crab, crab meats obtained during commercial processing, and from retail crab meat samples. each microbial isolate was then identified to the genus level employing the revised replica plating procedure. microbial groups most commonly isolated from crab meat were, in the order of predominance, moraxella, pseudomonas, acinetobacter, arthrobacter, micrococcus, flavobacterium-cytophaga, and ba ...19751096824
sequential growth of bacteria on crude oil.by modification of the enrichment culture procedure three bacterial strains capable of degrading crude oil in sea water were isolated in pure culture, up-2, up-3, and up-4. strain up-2 appears to be highly specialized for growth on crude oil in sea water since it showed strong preference for oil or oil degradation products as substrates for growth, converted 66% of the oil into a form no longer extractable by organic solvents, quantitatively degraded the paraffinic fraction (gas chromatographic ...19751096820
cleavage map of bacteriophage phix174 rf dna by restriction enzymes.phix rf dna was cleaved by restriction enzymes from haemophilus influenzae rf (hinf i) and haemophilus haemolyticus (hha. i). twenty one fragments of approximately 25 to 730 base pairs were produced by hinf i and seventeen fragments of approximately 40 to 1560 base pairs by hha i. the order of these fragments has been established by digestion on haemophilus awgyptius (hae iii) and arthrobacter luteus (alu i) endonuclease fragments of phix rf with hinf i and hha1. by this method of reciprocal dig ...19761085927
the genetic map of bacteriophage phix174 constructed with restriction enzyme fragments. 19761084616
improvements of the membrane filter method for dna:rrna hybridization.we describe and recommend the following improvements of dna:rrna membrane filter hybridization methods. one of our aims was to avoid dna release from filter discs during hybridization. 1. our hybridization conditions are 2 ssc in aq. dest., with 20% formamide, 50 c, overnight for 16 hr. 2. duplexing is over in 8-10 hr. 3. formamide has to be very pure (o.d. less than or equal to 0.2/cm light path at 270 nm). 4. rnaase treatment: 250 mug/5 ml 2 ssc/filter at 37 c for 1 hr. 5. our conditions for s ...19751082296
arthrobacter luteus restriction endonuclease cleavage map of phi chi 174 rf dna. 19751081295
identification of the cultivable bacteria in dental plaque from the beagle dog.fifteen different genera of microorganisms were isolated from the 27-month-old plaque of two beagle dogs. they were as follows: pseudomonas, proteus, neisseria, escherichia, staphylococcus, streptococcus, lactobacillus, nocardia, actinomyces, corynebacterium, micrococcus, microbacterium, brevibacterium, arthrobacter, and kurthia. the dog plaque was characterized by a low number of streptococci and the presence of several noncarbohydrate-fermenting organisms.19761069758
action of chondroitinases. ii. numerical calculation of the degree of multiple attack.further investigation was carried out on the action patterns of two chondroitinase-ac [ec 4.2.2.5.] preparations obtained from arthrobacter aurescens and flavobacterium heparinum. to infer the action patterns of the chondroitinases, we proposed a new method for the calculation of the degree of multiple attack, based on the concept established by robyt and french ((1967) arch. biochem. biophys. 122, 8-16). it was shown that the degree of multiple attack (dm) is represented by the ratio of the ini ...19761018015
[preliminary observations on the characterization of a microbial strain isolated from mud and active in saline medium with added phenanthrene]. 19761016622
microbiological degradation of bile acids. the conjugation of a certain cholic acid metabolite with amino acids in corynebacterium equi.1. (4r)-4[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (ii) could not be utilized by arthrobacter simplex, even though the acid was one of the metabolites formed from cholic acid (i) by this organism. therefore the further degradation of the acid (ii) by corynebacterium equi was investigated to identify the intermediates involved in the cholic acid degradation. 2. the organism, cultured in a medium containing the acid (ii) as the sole source of carbon, ...19761016254
macromolecular synthesis and cell division during morphogenesis of arthrobacter crystallopoietes.the sphere-rod-sphere morphology cycle of arthrobacter crystallopoietes was accompanied by changes in the rate of growth and the rates of dna, rna and protein synthesis. the patterns of macromolecule synthesis resembled those found in other bacteria during a step-up followed by a step-down in growth rate. during the step-up in growth spherical cells grew into rods and macromolecules were synthesized in the absence of cell division. during step-down, successive rounds of septation produced progre ...19761015960
induction of d-6-hydroxynicotine oxidase in resting cells of arthrobacter oxidans.resting cell suspensions of arthrobacter oxidans were shown to synthesize the inducible enantiozyme, d-6-hydroxynicotine oxidase, in the presence of d-nicotine or d-6-hydroxynicotine. the corresponding l-enantiomers, as well as gamma-methylaminopropyl-(6-oh-pyridyl-3)-ketone, which is the product of the reaction catalyzed by the enzyme, were ineffective as inducers. l-6-hydroxynicotine inhibited induction by d-nicotine and d-6-hydroxynicotine while l-nicotine inhibited induction by d-6-hydroxyni ...19761015955
[degradation of 4-chlorobenzoic acid by an arthrobacter species (author's transl)].an arthrobacter sp. growing on 4-chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. protocatechuic acid is further attacked by "meta"-cleavage. during growth of the arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from the medium, suggesting the involvement of the "ortho"-cleavage pathway. the enzymes both for the "meta"- and the "ortho"-cleavage pathway are inducible.19761015950
growth and pigment production by arthrobacter pyridinolis n. sp.a new bacterium capable of growing on 2-hydroxypyridine as sole source of carbon and nitrogen was isolated from soil. during its growth on solid medium, approximately 50% of this substrate was converted to a brilliant blue crystalline pigment which was deposited extracellularly in the colony mass. the pigment was identical to that produced by arthrobacter crystallopoietes during its growth on 2-hydroxypyridine. the new isolate exhibited the typical cycle of morphogenesis characteristic of the ge ...19761015948
conversion of (+/-)-synephrine into p-hydroxyphenylacetaldehyde by arthrobacter synephrinum. a novel enzymic reaction.a partically purified enzyme from arthrobacter synephrinum was found to catalyse the conversion of (+/-)-synphrine into p-hydroxyphrenylacetaldehyde and methylamine. the enzyme is highly specific for synephrine and is distinctly different from monoamine oxidase.19761008837
[survival of epiphytic microorganisms following sublimation dehydration and storage].epiphytic microorganisms belonging to the genera pseudomonas, agrobacterium, achromobacter, flavobacterium, mycobacterium, mycococcus, nocardia, corynebacterium, arthrobacter, bacillus, and microbacterium were dehydrated by sublimation employing two suspension media, and were stored at 3-5 degrees c during 1-2.5 years. survival of epiphytic microorganisms upon dehydration by sublimation and storage was found to depend on their taxonomy (genus, species, strain) and the composition of the suspensi ...19761004279
[effect of various carbon sources on cholinesterase formation by arthrobacter simplex var. cholinesterasus].the effect of carbohydrates, aromatic alcohols, choline and acetylcholine on the biomass production and biosynthesis of choline esterase was studied with arthrobacter simplex var. cholinesterasus. fructose was found to be the best carbon source for the biomass accumulation and synthesis of choline esterase. almost the same amount of the enzyme was produced on media with glucose and maltose as on the medium with fructose though the biomass yield was much lower. on the contrary, the biomass produc ...19761004262
4-keto-gamma-carotene(beta, psi-caroten-4-one), the major pigment of an arthrobacter sp. 19761000359
[degradation of (-)-ephedrine by arthrobacter globiformis (author's transl)].a bacterium which grows on and completely degrades ephedrine as the only source of carbon was isolated from soil. this bacterium was identified as arthrobacter globiformis. from the culture medium the following metabolites were isolated: 1-hydroxy-1-phenyl acetone, benzoic acid, pyrocatechol, cis-cis-muconic acid and methylamine. the mechanism of metabolism is discussed and compared with that in mammals.1976998048
the nucleotide sequence of a dna fragment, 71 base pairs in length, near the origin of dna replication of bacteriophage 0x174.part of the nucleotide sequence of a restriction fragment covering the origin of phix174 dna replication 1 has been determined. the fragment a7c was obtained by digestion of phix174 rf dna by the restriction enzyme from arthrobacter luteus, alu 1. it was further cleaved into two fragments, one large and one small, by the action of the restriction enzyme from haemophilus aegyptius, hae 111. the nucleotide sequence of the small fragment has been determined by analysis of the transcription products ...1976995652
[effect of nitrogen source on growth of arthrobacter simplex and its biosynthesis of cholinesterase].the effect of organic and inorganic forms of nitrogen on biomass accumulation and cholinesterase synthesis was studied with arthrobacter simplex var. cholinesterasus. the culture assimilates nitrogen of ammonium compounds better than other forms of inorganic nitrogen; the best nitrogen source for biosynthesis of cholinesterase is ammonium phosphate. nitrogen of nitrates is not assimilated. the amount of biomass is almost twice as high on the medium with peptone, casein or casein hydrolysate as o ...1976979679
on the regulation of histidinol dehydrogenase induction in arthrobacter histidinolovorans. 1976971316
ring hydroxylation of p-chlorophenylacetate by an arthrobacter strain.resting cell suspensions of a strain of arthrobacter grown on phenylacetate converted p-chlorophenylacetate to two products. one of the products was identified as 4-chloro-3-hydroxyphenylacetate.1976970937
motility as a morphogenic character in the genus arthrobacter.motility in arthrobacter atrocyaneus, a. citreus, and a. simplex was found to correlate with the morphogenic cycle of these organisms. the percentage of the a. atrocyaneus and a. simplex populations that were flagellated at a given time during the growth cycle differed significantly from that of the normorphogenic pseudomonas aeruginosa population. flagellation in a. atrocyaneus was shown to be dependent upon the morphogenic cycle rather than upon growth. the commitment to flagellar synthesis in ...1976956128
nalpha-acetylfusarinines: isolation, characterization, and properties.the isolation, purification, and structural elucidation of a new group of naturally occurring hydroxamic acids are described. these compounds are produced by an unidentified species of penicillium when grown on iron deficient medium. they are closely related structurally to the fusarinines, whose basic structure contains ndelta-(cis-5-hydroxy-3-methylpent-2-enoyl)-ndelta-hydroxy-l-ornithine, but the alpha-amino groups in the new compounds are protected by nalpha acetyl groups. the most complex o ...1976949471
[current achievements in the study of the structure and specificity of flavin enzymes]. 1976944972
soybean flower-to-seed movement of epiphytic bacteria.epiphytic tracer bacteris (a pseudomonas sp. and an arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. seed from two of these pods also carried tracers.1976943220
microbiological degradation of bile acids. the preparation of some hypothetical metabolites involved in cholic acid degradation.1. to identify the intermediates involved in the degradation of cholic acid, the further degradation of (4r)-4-[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (iva) by arthrobacter simplex was attempted. the organism could not utilize this acid but some hypothetical intermediate metabolities of compound (iva) were prepared for later use as reference compounds. 2. the nor homologue (iiia) and the dinor homologue (iiib) of compound (iva) were prepared by e ...1976942388
degradation of alpha-linked d-gluco-oligosaccharides and dextrans by an isomalto-dextranase preparation from arthrobacter globiformis t6. 1976938503
[oxidation and assimilation pathways of methylated amines in arthrobacter globiformis].the oxidation by arthrobacter globiformis b-175 of tertiary, secondary, and primary amines is catalyzed by specific induced enzymes. trimethylamine is oxidized to dimethylamine via trimethylamine n-oxide by monooxygenase and demethylase. dimethylamine is converted to methylamine by another monooxygenase. methylamine is oxidized to ammonia and formaldehyde by aminoxidase. formaldehyde is assimilated via ribulose monophosphate cycle, which is confirmed by the presence of hexose-phosphate synthase ...1976933867
natural paucity of anaplerotic enzymes: basis for dependence of arthrobacter pyridinolis on l-malate for growth.previous work has shown that in arthrobacter pyridinolis the transport systems for glucose and several amino acids are respiration coupled, with malate oxidation occurring concomitantly with transport. the requisite malate has to be supplied exogenously, so that growth on glucose or certain amino acids only occurs if malate is also present in the medium. these and other data suggested that a. pyridinolis might be deficient in anaplerotic enzymes, which maintain intracellular levels of dicarboxyl ...1976931946
arthrobacter sialophilus sp. nov.; a neuraminidase-producing coryneform.a new species of arthrobacter is described for which the name a. sialophilus is proposed. the organism was obtained by elective culture from soil using collocalia mucoid, and can readily be induced in replacement minimal media with appropriate substrates, to form the enzyme neuraminidase. it undergoes sphere-rod-sphere morphogenesis, is gram-variable, non-motile, aerobic, catalase-positive, oxidase-negative, fails to release acid or gas from saccharides, has a gc content of 56.0 mol % and can gr ...1977922607
the mechanism of dextransucrase action. activation of dextransucrase from streptococcus mutans omz 176 by dextran and modified dextran and the nonexistence of the primar requirement for the synthesis of dextran. 1977921284
action of chondroitinases. iii. ionic strength effects and kinetics in the action of chondroitinase ac. 1977914791
macromolecular synthesis and degradation in arthrobacter during periods of nutrient deprivation.cells of arthrobacter atrocyaneus and a. crystallopoietes, harvested during their exponential phase, were starved in 0.03 m phosphate buffer (ph 7.0) for 28 days. during this time, the cells maintained 90 to 100% viability. experimental results were similar for both organisms. total cellular deoxyribonucleic acid was maintained. measurable degradation rates for deoxyribonucleic acid as determined by radioisotope techniques were not observed, and only during the initial hours of starvation could ...1977914778
a new enzymatic method for determination of serum choline-containing phospholipids.a new enzymatic method is presented for the determination of serum choline-containing phospholipids with a combined enzymatic method using phospholipase d (from streptomyces species), choline oxidase (from arthrobacter species) and peroxidase. the method is reproducible, and the results correlate well with those obtained by the conventional digestion method (hoeflmayr, j. and fried, r. (1966) med. ernaehr. 7, 9-10). the method affords better specificity, requires a smaller quantity of the sample ...1977890967
photodynamic induction of a bacterial cell surface polypeptide.the photodynamic action of several dyes on cells of a bacterium, tentatively identified as a species of arthrobacter, resulted in remarkable stimulation of synthesis of a polypeptide 21,000 daltons in mass. this polypeptide resides on the cell surface and can be solubilized by sodium dodecyl sulfate without lysis of the cells. chlorophyllin and rose bengal are effective in inducing synthesis of the polypeptide in proportion to their ability to sensitize the photooxidation of histidine. etiolated ...1977885841
microbiological degradation of bile acids. the preparation of hexahydroindane derivatives as substrates for studying cholic acid degradation.relatively large amounts of 3-(3aalpha-hexahydro-7abeta-methyl-1,5-dioxoindan-4alpha-yl)propionic acid (iib), which is believed to be one of the intermediates involved in the degradation of cholic acid (i), were needed to identify is further degradation products. a simple method for the preparation of this compound was then investigated. arthrobacter simplex could degrade-3-oxoandrost-4-ene-17beta-carboxylic acid (iiia) to 3-(1beta-carboxy-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-4alpha-yl)pro ...1977883962
[acetylcholine metabolism in arthrobacter simplex var. cholinesterasus]. 1977882022
inabilities as an immunoadjuvant of cell walls of the group b peptidoglycan types and those of arthrobacters.the cell walls from several bacterial species whose peptidoglycans are the group b types (schleifer and kandler) and those of two arthrobacters were shown to be inactive or only weakly active as an immunoadjuvant in both induction of delayed-type hypersensitivity and stimulation of circulating antibody levels to ovalbumin when administered to guinea pigs as a water-in-oil emulsion, in sharp contrast to the adjuvant-active cell walls of the group a peptidoglycan types which were previously studie ...1977880204
nitrification in histosols: a potential role for the heterotrophic nitrifier.insufficient populations of nitrosomonas and nitrobacter were found in a pahokee muck soil (lithic medidaprit) to account for the nitrate concentration observed. to determine if heterotrophic nitrifiers could account for some of this discrepancy, a method was developed to measure the levels of heterotrophic nitrifiers in soil. a population of 4.1 x 10(5) arthrobacter per g of dry fallow soil, capable of producing nitrite and/or nitrate from reduced nitrogenous compounds, was observed. amendment ...1977869537
microbiological characteristics of pacific shrimp (pandalus jordani).microorganisms associated with pacific shrimp (pandalus jordani) were isolated and identified. those on the iced raw shrimp, which yielded an average count of 1.6 x 10(6), were predominantly moraxella, pseudomonas, acinetobacter, arthrobacter, and flavobacterium-cytophaga spp. the blanching and peeling reduced the microbial level to 3.3 x 10(4) and also selectively eliminated moraxella spp. the microbial flora changed after each processing sequence, and the heat sensitivity and growth characteri ...1977869532
oxidation of linear terpenes and squalene variants by arthrobacter sp.cells of arthrobacter sp. that had been isolated from soil were used to study oxidation of some linear terpenes and squalene variants. the cells oxidized geraniol, nerol, and farnesol to the corresponding aldehydes, with partial conversion of the geometrical isomerism of the alpha,beta-double bond. the squalene variant, squalene-2,3-oxide, was cleaved to 9,10-epoxygeranylacetone and geranylacetone. squalene-2,3-22,23-dioxide was cleaved to 9,10-epoxygeranylacetone. these products were optically ...1977869527
electron microscopic study of cell surface rings during cell division and morphogenesis of arthrobacter crystallopoietes.the whole cell ultrastructure during cell division and morphogenesis of arthrobacter crystallopoietes was monitored using electron microscopic techniques. glucose-grown spherical cells were inoculated into succinate-based medium. in this medium, the organism undergoes a morphogenetic cycle consisting of elongation of spheres to rods, exponential growth as rods, and fragmentation of rods to spherical cells. raised bands or rings that encircled the cells were evident on the cell surface of both sp ...1977863857
distribution of xanthine oxidase and xanthine dehydrogenase specificity types among bacteria.a diverse collection of xanthine-metabolizing bacteria was examined for xanthine-, 1-methylxanthine-, and 3-methylxanthine-oxidizing activity. both particulate and soluble fractions of extracts from aerobically grown gram-negative bacteria exhibited oxidation of all three substrates; however, when facultative gram-negative bacteria were grown anaerobically, low particulate and 3-methylxanthine activities were detected. gram-positive and obligately anaerobic bacteria showed no particulate activit ...1977863854
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