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[the effects of tea polyphenols on the adherence of cariogenic bacterium to the collagen in vitro].in the first phase of our study, it has been proved that tea polyphenols (tp) can inhibit the preliminary adherence of oral cariogenic bacterium to salivary acquired pellicle. this study is carried on to investigate the effects of tea polyphenols on the preliminary adherence of main cariogenic bacterium to collagen i and reveal the anticarious mechanisms of tea polyphenols further.200012539657
[separation and identification of polyclonal antibodies against type ii fimbriae of actinomyces viscosus 5951].this study was aimed to prepare the polyclonal antibodies against type ii fimbriae of actinomyces viscosus 5951.200015014789
[a preliminary study on fimbriae subunits and molecular weight of actinomyces viscosus 5951]objective:to preliminarily analyse the subunits and molecular weight of fimbriae of actinomyces viscosus 5951.methods:to do the immunoblotting test of the only ii fimbriae type of mutant strain of t14v.results:it showed that there was only one 31ku band,which indicated that the fimbriae of actinomyces viscosus 5951 was composed of the same subunits of 31ku molecular weight. conclusion:according to the previous reports and results of present study,the molecular weight of fimbriae of actinomyces v ...200015014834
photoactive porphyrin derivative with broad-spectrum activity against oral pathogens in vitro.photodynamic therapy (pdt) has historically been used as a means to treat cancerous tumors but has recently been used to kill bacterial cells through the use of targeted photosensitizers. pdt is a potential adjunct to scaling and root planing in the treatment of periodontal disease. however, the effectiveness of porphyrin derivatives against microorganisms has been limited because some gram-negative bacteria are refractory to photodynamic treatment with these agents. we have designed a porphyrin ...200011083641
studies concerning the glucosyltransferase of streptococcus sanguis.we have shown in previous studies that the glucosyltransferase (gtf) enzymes of streptococcus mutans have distinct properties when adsorbed to a surface. in the present study, we compared the activity of gtf from streptococcus sanguis, designated gtfss, in solution and on the surface of saliva-coated hydroxyapatite (sha) beads, and determined the ability of its product glucan to support the adherence of oral microorganisms. gtf from s. sanguis 804 nctc 10904 was purified from culture supernatant ...200010867431
photomechanical drug delivery into bacterial biofilms.to investigate whether photomechanical waves generated by lasers can increase the permeability of a biofilm of the oral pathogen actinomyces viscosus.200010870983
in vitro colonisation of acrylic resin denture base materials by streptococcus oralis and actinomyces viscosus.the aim of the study was to compare the attachment of two typical strains of oral bacteria to four denture base materials.200010945186
identification of periodontal disease-associated bacteria in the "plaque-free zone".subgingival plaque bacteria live within a biofilm covered with glycocalyx, and little is known of the bacterial species associated with biofilm formation at the bottom of human periodontal pockets, the so-called "plaque-free zone"(pfz).200010972648
humoral immunity host factors in subjects with failing or successful titanium dental implants.treatment with titanium dental implants is in general successful. however, an unknown number of implants do not integrate and are removed either by exfoliation or at the time of second stage surgery. it would be of importance to identify subjects at risk and predict early implant failure.200011140553
effect of three mouthrinses, containing amine/stannous fluoride, herbal extracts or emser salt on the growth of oral bacteria--an in vitro study.clinical studies have shown the efficacy of mouthrinses in reducing plaque accumulation and inflammation of oral tissues. the aim of this in vitro study was to compare the effect of three mouthrinses: meridol, an organic amine/ stannous fluoride solution; parodontax, containing herbal ingredients; and an 0.8 % emser salt solution, on the growth of oral bacteria and dental plaque.200011147996
antimicrobial activity of tanzanian chewing sticks against oral pathogenic microbes.methanol extracts from the bark and wood of ten plants used as chewing sticks in morogoro region, in tanzania, were tested for their ability to inhibit the growth of cariogenic bacteria, streptococcus mutans , actinomyces viscosus and a yeast candida albicans . screening for antimicrobial activity was done by the agar-hole diffusion method, and minimum inhibitory concentrations (mics) were determined by the agar dilution method. extracts from seven out of the ten plants showed varying degrees of ...200021214468
the role of fructans on dental biofilm formation by streptococcus sobrinus, streptococcus mutans, streptococcus gordonii and actinomyces viscosus.dental plaque biofilm plays a pivotal role in the progression of dental diseases. polysaccharides are of great importance in the ecology of the dental biofilm. we studied the effect of fructans, glucans and a mixture of both fructans and glucans, synthesized in situ by immobilized fructosyltransferase or glucosyltransferase, on the adhesion of streptococcus sobrinus, streptococcus mutans, streptococcus gordonii and actinomyces viscosus to hydroxyapatite beads coated with human saliva (sha). the ...200111179653
reduced fimbria-associated activities of porphyromonas gingivalis induced by recombinant fimbrial expression.the adhesion properties of the recombinant fimbriae (r-fimbriae) recovered from a yh522 transformant of porphyromonas gingivalis which harbors a chimeric plasmid, pyhf2, containing the fima gene of strain 381 were compared with those of the endogenous fima fimbriae of strain 33277. the adhesion level of the r-fimbriae to actinomyces viscosus was clearly lower than that of the endogenous fimbriae. in addition, the r-fimbriae were shown to lack some minor components detectable in the endogenous fi ...200111179655
endocarditis due to actinomyces viscosus.we report a case of endocarditis caused by actinomyces viscosus in a previously healthy young adult with no known identifiable portal of entry. infective endocarditis caused by a viscosus is uncommon; two cases of endocarditis caused by this species have been previously reported. primary actinomycotic endocarditis has been previously reviewed in 1993; we provide a review of additional cases since that report. in comparing our case with the other reported cases, we found that actinomycotic endoca ...200111235043
antibacterial properties of dentin bonding systems, polyacid-modified composite resins and composite resins.this study examined the antibacterial activities of the bonding systems syntac, ebs and scotchbond 1, the polyacid-modified composite resins hytac and compoglass, and the composite resins tetric, z100 and scalp-it. they were evaluated using the cariogenic bacteria streptococcus mutans, lactobacillus salivarius, streptococcus sorbinus and actinomyces viscosus in vitro with a modified cylinder drop plate agar diffusion assay. all adhesives of the dentin bonding systems and the polyacid-modified co ...200111298264
antibacterial activity of particulate bioglass against supra- and subgingival bacteria.particulate bioglass is a bioactive material used in the repair of periodontal defects. this material undergoes a series of surface reactions in an aqueous environment which lead to osseointegration. the aim of this study was to determine whether these reactions exerted an antibacterial effect on a range of oral bacteria. streptococcus sanguis, streptococcus mutans and actinomyces viscosus were suspended in nutrient broth (nb), artificial saliva (as) or dulbecco's modified eagle medium plus 10% ...200111374470
comparison of corrosion behaviour in presence of oral bacteria.the aim of this study was to compare the resistance of the corrosion of dental alloys in a solution containing oral bacteria named actinomyces viscosus (atcc19246). in this paper, we explain the choice of this precise species of bacteria, then specify its culture in artificial saliva and the experimental precautions needed to avoid the pollution by other bacteria. the electrochemical behaviour of two dental alloys (ni-cr alloy and gold-based alloy) was investigated by electrochemical means in st ...200111456067
cariogenic actinomyces identified with a beta-glucosidase-dependent green color reaction to gardenia jasminoides extract.the oral bacteria actinomyces naeslundii and actinomyces viscosus are known to contribute to the initiation and progression of human dental caries, especially root caries. we report that both a. naeslundii and a. viscosus react with a component in the gardenia jasminoides extract to produce a distinct green product. this green color reaction was found to be dependent on the bacterial beta-glucosidase. the reaction is specific for cariogenic actinomyces, and it can detect as few as 10(4) cells of ...200111474036
properties of streptococcus sanguinis glucans formed under various conditions.the aim of our study was to determine whether the structure of glucans formed by glucosyltransferase from streptococcus sanguinis (gtfss) on a surface differ from those formed in solution and to explore the effects of antiserum to gtfs, control normal rabbit serum, starch hydrolysates (sth) and dextran on s. sanguinis (gtfss) glucan. linkage analyses showed that solution-formed glucans are predominantly alpha-1,6-linked and have a small amount of alpha-1,3-linked glucose. surface-formed glucans ...200111125200
roles of fructosyltransferase and levanase-sucrase of actinomyces naeslundii in fructan and sucrose metabolism.the ability of actinomyces naeslundii to convert sucrose to extracellular homopolymers of fructose and to catabolize these types of polymers is suspected to be a virulence trait that contributes to the initiation and progression of dental caries and periodontal diseases. previously, we reported on the isolation and characterization of the gene, ftf, encoding the fructosyltransferase (ftf) of a. naeslundii wvu45. allelic exchange mutagenesis was used to inactivate ftf, revealing that ftf-deficien ...200111500409
in vitro antimicrobial activities of bakuchiol against oral microorganisms.bakuchiol was isolated from the seeds of psoralea corylifolia, a tree native to china with various uses in traditional medicine, followed by extraction with ether and column chromatography combined with silica gel and octyldecyl silane. in this study, the antimicrobial activities of bakuchiol against some oral microorganisms were evaluated in vitro. the cell growth of streptococcus mutans was inhibited in a bakuchiol concentration-dependent manner, and growth of s. mutans was completely prevente ...200111600349
[experimental study of bacteriostatic activity of chinese herbal medicines on primary cariogenic bacteria in vitro].to screen some chinese herbal medicines for their inhibitory activity on cariogenic bacteria, and investigate their active ingredients, and measure their minimum inhibitory concentration (mic), minimum bactericidal concentration (mbc).200111769659
different type 1 fimbrial genes and tropisms of commensal and potentially pathogenic actinomyces spp. with different salivary acidic proline-rich protein and statherin ligand specificities.actinomyces spp. exhibit type 1 fimbria-mediated adhesion to salivary acidic proline-rich proteins (prps) and statherin ligands. actinomyces spp. with different animal and tissue origins belong to three major adhesion types as relates to ligand specificity and type 1 fimbria genes. (i) in preferential acidic-prp binding, strains of actinomyces naeslundii genospecies 1 and 2 from human and monkey mouths displayed at least three ligand specificities characterized by preferential acidic-prp binding ...200111705891
an unusual case of actinomycosis.we describe an unusual case of actinomycosis that appeared as a nasal mass. the diagnosis was established when actinomyces viscosus was isolated from pus obtained from the right maxillary antrum by intranasal antrostomy. the patient was treated with intravenous crystalline penicillin and made an uneventful recovery within 10 days.200211816389
upregulation of co-stimulatory molecule expression and dendritic cell marker (cd83) on b cells in periodontal disease.t cells and their cytokines are well known for their important role in the pathogenesis of periodontitis. to date, the role of antigen presenting cells (apcs), which are known to be critical in the regulation of t cell response, has been poorly investigated in periodontitis. in this study, we analyzed the expression of co-stimulatory molecules (cd80 and cd86) and cd83, which is a marker of mature dendritic cells, on gingival cells that were isolated from severe periodontitis tissues, with the us ...200212113551
bacterial colonization of zirconia ceramic surfaces: an in vitro and in vivo study.the microbial colonization of new ceramic materials developed for abutment manufacturing was assessed.200212507238
[effect of para-aminobenzoic acid on the growth of actinomyces viscosus].to examine the effects of para-aminobenzoic acid (paba) on the growth of actinomyces viscosus.200212575187
[the in vitro study of the effects of 11 kinds of traditional chinese medicine on the growth and acid production of actinomyces viscosus].to assess the effects of different natural medicines on the growth and acid production of actinomyces viscosus, thus making preparations for screening an effective agent to mediate the balance of oral microflora.200212575200
comparison of antibacterial activity of simplified adhesive systems.to determine and compare the intrinsic antibacterial activity of commercially available simplified adhesive systems.200212693381
[the in vitro study on polistes mandarinus' effects on cariogenic bacteria].to study the role of polistes mandarinus on the growth,acid generation and water insoluble glucan(wig) production of streptococcus mutans, streptococcus sanguis and actinomyces viscosus as a preparation for screening an effective agent ot medicate the balance of oral microflora.200214983338
[effect of para-aminobenzonic acid on cell-surface hydrophobicity of actinomyces viscosus].to study the effect of para-aminobenzonic acid (paba) on cell-surface hydrophobicity of actinomyces viscosus.200312910699
actinomyces spp. in supragingival plaque of ethnic chinese preschool children with and without active dental caries.very limited molecular epidemiological data are available on the role of actinomyces spp. in the pathogenesis of caries in the primary dentition. therefore, we investigated their distribution in supragingival plaque of ethnic chinese preschool children from singapore and hong kong, either with or without active caries. plaque samples were taken from intact interproximal enamel areas using dental floss. bacterial genomic dna of each sample was extracted and variable regions of 16s ribosomal dna a ...200312925831
induction of suppressor cells following mucosal presentation of actinomyces viscosus in mice.mucosal presentation of actinomyces viscosus results in antigen-specific systemic immune suppression, known as oral tolerance. the aim of the present study was to determine the mechanism by which this oral tolerance is induced. dba/2 mice were gastrically immunized with a. viscosus. serum, peyer's patch (pp) and spleen cells were transferred to syngeneic recipients which were then systemically challenged with the sameia. viscosus strain. to determine antigen-specificity of cells from gastrically ...200312930525
production of monoclonal antibody inhibiting dipeptidylaminopeptidase iv activity of porphyromonas gingivalis.porphyromonas gingivalis is a gram-negative anaerobic bacterial species implicated as an important pathogen in the development of adult periodontitis. we previously cloned a gene encoding dipeptydilaminopeptidase iv (dapiv) from p. gingivalis. in the present study, for immunological diagnosis and development of passive immunization, we produced a mouse monoclonal antibody (mab) capable of inhibiting the dapiv activity of p. gingivalis using highly purified recombinant dapiv as an immunogen. the ...200312954099
microbiology and management of endodontic infections in children.the first step in the origination of caries is the formation of a dental plaque. dental caries can lead to destruction of enamel and dentin resulting in bacterial invasion of the pulp. invasion of the pulp and the periapical areas can promote the development of dento-alveolar abscess and spread of the infection to other anatomical areas. several oral acid producing aerobic and anaerobic bacteria, including streptococcus mutans, lactobacillus acidophilus, and actinomyces viscosus, are capable of ...200314604136
effect of sustained-release chlorhexidine varnish on streptococcus mutans and actinomyces viscosus in orthodontic patients.this study evaluated the effect of sustained-release chlorhexidine varnish on orthodontic patients. ten children, ages 10 to 16 years, participated. bacterial levels of streptococcus mutans and actinomyces viscosus and total counts were evaluated in sputum samples. these counts were evaluated at 4 stages: before orthodontic treatment, at least 2 weeks after bonding of the brackets, 1 week after application of chlorhexidine varnish, and 3 weeks after application of chlorhexidine varnish. increase ...200312637907
expression of mhc class ii, cd70, cd80, cd86 and pro-inflammatory cytokines is differentially regulated in oral epithelial cells following bacterial challenge.oral epithelium may play a regulatory role in local immune responses when interacting with bacteria. the present study was undertaken to investigate the effects of selected bacterial pathogens found in periodontal and endodontic infections on oral epithelial cells. expression of cell surface molecules (major histocompatibility complex (mhc) class ii, cd54, cd70, cd80 and cd86) and secretion of inflammatory cytokines (interleukin (il)-1beta, il-6, and tumor necrosis factor (tnf)-alpha) in respons ...200314622340
a rapid procedure to ascertain the antimicrobial efficacy of oral care formulations.a rapid method examining the antimicrobial efficacy of oral care formulations with alamar blue, an oxidation-reduction dye with fluorescent end-points, is described. significant correlations between increasing viable plate counts of the oral bacteria actinomyces viscosus, streptococcus sanguis, streptococcus mutans and actinobacillus actinomycetemcomitans and increased alamar fluorescence were noted. metabolically active bacteria reduced alamar with the reduced dye found in the cell-free filtrat ...200314622343
multiple bacteria in aortic aneurysms.the purpose of the present study was to reexamine the possibility that bacteria, particularly anaerobes, are present in aortic aneurysms.200314681645
effect of porphyromonas gingivalis vesicles on coaggregation of staphylococcus aureus to oral microorganisms.vesicles from the outer membrane of porphyromonas gingivalis have the ability to aggregate a wide range of streptococcus spp., fusobacterium nucleatum, actinomyces naeslundii, and actinomyces viscosus. we found that in the presence of p. gingivalis vesicles, staphylococcus aureus coaggregated with streptococcus spp., and the mycelium-type candida albicans, but not the yeast type. autoaggregation of s. aureus in the presence of p. gingivalis vesicles is inhibited by l-arginine, l-lysine, and l-cy ...200314756532
bacteriophage isolation from human saliva.to detect bacteriophages for gram-positive oral pathogens in human saliva.200312485342
[comparison of the antibacterial activity on oral pathogens among six types of nano-silver base inorganic antibacterial agents].to compare the antibacterial activity on oral pathogens among six types of nano-silver base inorganic antibacterial agents.200314966611
generation of gingival t cell lines/clones specific with porphyromonas gingivalis pulsed dendritic cells from periodontitis patients.it is well documented that in periodontitis lesions, most infiltrated gingival t cells are antigen-specific memory t cells. these cells play an important role as regulators and effector cells in the pathogenesis of periodontitis. in this study, we used dendritic cells (dcs) as antigen-presenting cells to generate human gingival t cell lines and clones specific for porphyromonas gingivalis from periodontitis patients.200312753363
[effect of metabolic substances of oral actinomyces on candida albicans].actinomyces naeslundii, actinomyces viscosus and candida albicans are associated with root cavity. the aim of this study was to determine, in vitro, the effect produced by the metabolic substances elaborated by actinomyces naeslundii and actinomyces viscosus on candida albicans. the strains were isolated of saliva. there were used the double plaque diffusion method (dpdm) and the method of radial diffusion (mrd). the effect of the time of incubation and of different concentrations of metabolic s ...200415458360
actinomycosis in a one-humped camel (camelus dromedarius).an actinomycotic granuloma caused by actinomyces viscosus is reported in a dromedary camel. two hard, cutaneous, large granulamatous nodules were present on both sides of the postero-ventral side of the mandible exhibiting exudation and necrosis. after radical excision of the lesion, the daily treatment with penicillin-streptomycin combination was continued for 4 weeks. about 8 and 24 weeks from the initial treatment, no new nodules were noticed.200415533120
roles of arg- and lys-gingipains in coaggregation of porphyromonas gingivalis: identification of its responsible molecules in translation products of rgpa, kgp, and haga genes.arg- (rgp) and lys-gingipains (kgp) are two individual cysteine proteinases produced by porphyromonas gingivalis , an oral anaerobic bacterium, and are implicated as major virulence factors in a wide range of pathologies of adult periodontitis. coaggregation of this bacterium with other oral bacteria is an initial and critical step in infectious processes, yet the factors and mechanisms responsible for this process remain elusive. here we show that the initial translation products of the rgpa , ...200415576324
[dynamic observation of periodontal pathogens and cariogenic bacteria in modified chemostat using confocal laser scanning microscopy].to study dynamic relation between periodontal pathogens and cariogenic bacteria under analogous oral environment.200415061891
comparison of type 1 and type 2 cytokine production by mononuclear cells cultured with streptococcus mutans and selected other caries bacteria.a feature of pulpal immune responses is the predominance of type 1 cytokine mrna under shallow caries and a mixed (type 1/type 2) profile under deep caries. these results prompted an examination of the cytokine profiles induced by bacteria in shallow caries (streptococcus mutans and actinomyces viscosus) and deep caries (lactobacillus casei, pseudoramibacter alactolyticus, and prevotella intermedia). all isolates induced interferon-gamma and interleukin-10, whereas interleukin-4 and interleukin- ...200415107645
effects of low dose chlorhexidine mouthrinses on oral bacteria and salivary microflora including those producing hydrogen sulfide.clinical studies have demonstrated the considerable effects of chlorhexidine on dental plaque and oral microbiota as well as improvements in indices of oral health. this investigation examined the efficacy of lower concentrations of chlorhexidine.200415327643
the antimicrobial potential of 14 natural herbal dentifrices: results of an in vitro diffusion method study.increasing numbers of americans are using natural herbal products for general and oral health care. few of these products, however, have undergone rigorous testing, as evidenced by the limited amount of information on their safety and efficacy in the literature. the authors conducted an in vitro study to evaluate the antimicrobial potential of 14 natural herbal dentifrices.200415387052
improving protein pharmacokinetics by genetic fusion to simple amino acid sequences.the role of primary amino acid sequences in protein pharmacokinetics, an issue of relevance in both basic knowledge and biotechnology, was addressed here using as a starting point two repetitive antigens from the hemoflagellate trypanosoma cruzi that are known to stabilize their associated proteins in the bloodstream. a major drawback to their pharmacological application is that these repetitive sequences are highly immunogenic, being therefore the deletion of this characteristic desirable. base ...200414612434
antimicrobial effects of a new therapeutic liquid dentifrice formulation on oral bacteria including odorigenic species.the control of oral malodor is well-recognized in efforts to improve oral health. antimicrobial formulations can mitigate oral malodor, however, procedures to assess effects on oral bacteria including those implicated in halitosis are unavailable. this investigation examined the antimicrobial effects of a new liquid triclosan/copolymer dentifrice (test) formulation that demonstrated significant inhibition of oral malodor in previous organoleptic clinical studies. procedures compared antimicrobia ...200515449114
a rare case of primary actinomycosis of the breast caused by actinomyces viscosus: diagnosis by fine-needle aspiration cytology under ultrasound guidance.we report the case of a 27-year-old woman with primary actinomycosis of the breast. diagnosis was established by culture examination of specimen recovered by fine-needle aspiration cytology (fnac) under ultrasound guidance. to our knowledge, this is the first description in the literature of a case of primary actinomycosis of the breast caused by actinomyces viscosus. twenty-nine previous cases of primary actinomycosis of the breast have been published, but these were caused by the more common s ...200515647080
effects of cecropin-xj on growth and adherence of oral cariogenic bacteria in vitro.cecropin-xj belongs to cecropin-b, which is the most potent antibacterial peptide found naturally. the aim of this study was to investigate the effects of cecropin-xj on growth and adherence of oral cariogenic bacteria.200515667802
in-vitro evaluation of microleakage of an orthograde apical plug of mineral trioxide aggregate in permanent teeth with simulated immature apices.this in vitro study evaluated the seal created by varying depths of mineral trioxide aggregate (mta) plugs placed in an orthograde fashion in five groups of 10 teeth. one group received a 2 mm thick orthograde apical plug of mta, the second group a 5 mm apical mta plug, and the third group a 2 mm apical mta plug with a second 2 mm increment, 24 h later. the remaining portion of the canal in these groups was left unfilled. group four received a 2 mm mta plug that set for 24 h and the canal was th ...200515671823
[the effect of galla chinensis on the growth of cariogenic bacteria in vitro].the purples of this study was to investigate the role of different components of galla chinensis extract on the growth of 6 kinds of cariogenic bacteria, and to find out the most effective components of galla chinensis extract.200515804032
actinomyces viscosus endocarditis requiring aortic valve replacement.we report a case of primary actinomyces viscosus endocarditis, an unusual manifestation of actinomycosis, in a 43-year-old farmer with an indolent febrile illness. as has occurred in previous cases, diagnosis was delayed in part because blood isolates were misidentified. months later when she required aortic valve and root replacement, histologic exam of the diseased valve revealed branching filamentous organisms and the original blood isolates were retrospectively confirmed to be actinomyces vi ...200515845438
[an in vitro study on effect of nidus vespae extract on the acid production of three strains of oral bacteria].to inquire into the effect of different of nidus vespae extract (nve) on growth and acid production of streptococcus mutans, streptococcus sanguis and actinomyces viscosus.200515931873
in vitro binding interactions of oral bacteria with immobilized fructosyltransferase.the objective of the present study was to explore the role of immobilized fructosyltransferase (ftf) in adhesion process.200616553744
sialidase fusion protein as a novel broad-spectrum inhibitor of influenza virus infection.influenza is a highly infectious disease characterized by recurrent annual epidemics and unpredictable major worldwide pandemics. rapid spread of the highly pathogenic avian h5n1 strain and escalating human infections by the virus have set off the alarm for a global pandemic. to provide an urgently needed alternative treatment modality for influenza, we have generated a recombinant fusion protein composed of a sialidase catalytic domain derived from actinomyces viscosus fused with a cell surface ...200616569867
[on the relationship between exopolysaccharides and actinomyces viscosus in biofilms].to elucidate the biofilm structure of actinomyces viscosus and the spatial distribution of exopolysaccharides in it.200616608069
role of cd4 and cd8 t-cells in the induction of oral tolerance to actinomyces viscosus in mice.mucosal presentation of actinomyces viscosus results in the induction of antigen specific systemic suppressor cells in mice. the aim of the present study was to determine the phenotype of the suppressor cells responsible for the induction of oral tolerance to low doses of a. viscosus. when cd8 cell-depleted dba/2 mice were intragastrically immunized and systemically immunized with a. viscosus, the delayed type hypersensitivity response was suppressed but not the levels of antigen specific serum ...200616626371
effects of extracts of miswak and derum on proliferation of balb/c 3t3 fibroblasts and viability of cariogenic bacteria.this study examined the effects of extracts of two chewing sticks on proliferation of fibroblasts and viability of cariogenic bacteria.200616637906
in vitro assessment of antimicrobial peptides as potential agents against several oral bacteria.antimicrobial peptides are components of the innate immunity that play an important role in systemic and oral health.200616687459
the antibacterial effects of zinc ion migration from zinc-based glass polyalkenoate cements.zinc-based glass polyalkenoate cements have been synthesised and their potential use in orthopaedic applications investigated. zinc ions were released from the materials in a rapid burst over the first 24 h after synthesis, with the release rate falling below detectable levels after 7 days. cement-implanted bone samples were prepared and the released zinc was shown, using energy dispersive x-ray analysis, to penetrate from the cement into the adjacent bone by up to 40 microm. finally, the cement ...200616691345
antibacterial activities of inorganic agents on six bacteria associated with oral infections by two susceptibility tests.the antibacterial effects of six inorganic antibacterial agents were assessed using broth dilution and agar dilution tests on six pathogenic bacteria associated with oral infectious diseases: streptococcus mutans (atcc 25175), s. mutans (ingbritt), actinomyces viscosus (atcc 15987), lactobacillus casei (atcc 393), staphylococcus aureus (atcc 29213) and candida albicans (atcc 90028). the results of the broth dilution test were significantly lower than those of the agar dilution test (f=38.290; p< ...200616713190
the induction of oral tolerance to actinomyces viscosus in mice.to determine whether oral tolerance with the oral bacterium actinomyces viscosus was inducible in mice.200616792724
multiparameter assessments to determine the effects of sugars and antimicrobials on a polymicrobial oral biofilm.clinical studies indicate relationships between dental plaque, a naturally formed biofilm, and oral diseases. the crucial role of nonmicrobial biofilm constituents in maintaining biofilm structure and biofilm-specific attributes, such as resistance to shear and viscoelasticity, is increasingly recognized. concurrent analyses of the diverse nonmicrobial biofilm components for multiparameter assessments formed the focus of this investigation. comparable numbers of actinomyces viscosus, streptococc ...200617021225
the role of cd4+ and cd8+ t cells on antibody production by murine peyer's patch cells following mucosal presentation of actinomyces viscosus.the aim of this study was to determine the role of cd4 and cd8 cells on specific antibody production by murine peyer's patch (pp) cells after oral immunization with actinomyces viscosus in mice. female dba/2 mice were orally immunized with three low doses of heat-killed a. viscosus. sham-immunized mice served as a control group. mice were depleted of cd4 or cd8 cells by intraperitoneal injection of anti-cd4 or anti-cd8 antibodies daily for 3 days before oral immunization. one week after the last ...200617064401
antibacterial diterpenoids from sagittaria pygmaea.there were five new diterpenoids, 18-beta-d-3',4'-diacetoxyxylopyranosyl-ent-kaur-16-ene (1), 18-beta-l-3',5'-diacetoxyarabinofuranosyl-ent-kaur-16-ene (2), 18-beta-d-3',6'-diacetoxyglucopyranosyl-ent-kaur-16-ene (3), ent-isopimar-8(14),15-dien-19-oic acid (4), and 5alpha-hydroxy-ent-rosa-15-en-18-oic acid (5), isolated from the whole herb of sagittaria pygmaea. their structures and relative configurations were established based on spectroscopic studies, chemical methods, and x-ray crystallograp ...200717315313
[antimicrobial activity of nano-hydroxyapatite/polyamide 66 as a pulp capping agent in vitro].objective to investigate the in vitro antimicrobial activity of nano-hydroxyapatite/polyamide 66 composites (nha-pa66) as a pulp capping agent.200717375571
an in vitro quantitative antibacterial analysis of amalgam and composite resins.antibacterial properties of restorative dental materials such as amalgam and composite resins may improve the restorative treatment outcome. this study evaluates the antibacterial properties of three composite resins: z250, tetric ceram, p60 and a dental amalgam in vitro.200716996674
the inhibition of dentifrice containing the lotus leaf-derived inhibitor on periodontitis-related bacteria in vitro.to search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease.200717992914
in vitro antimicrobial efficiency of a mouthwash containing triclosan/gantrez and sodium bicarbonate.several antiseptic substances have been used as adjuncts to routine mechanical procedures of oral hygiene, based on their antimicrobial effects. the objective of this study was to assess in vitro the antimicrobial efficiency of a mouthwash containing triclosan/gantrez and sodium bicarbonate in comparison to both positive and negative controls. standard strain samples of escherichia coli, pseudomonas aeruginosa, actinomyces viscosus and bacillus subtilis were used. samples of streptococcus mutans ...200718060262
cutaneous actinomycosis: a rare case.cutaneous actinomycosis is a rare presentation. here we present a case of cutaneous actinomycosis with no history of trauma or systemic dissemination. the isolate was identified as actinomyces viscosus by standard methods. the isolate was found to be penicillin resistant by kirby bauer disc diffusion method. therefore, the patient was treated with cotrimoxazole and improved. thus, this case highlights the importance of isolation and susceptibility testing in actinomycotic infection. the sinuses ...200718087099
[study of the relationship between oral actinomyces and childhood caries].actinomyces are members of the indigenous oral microbiota which have been implicated in root caries, but their role in childhood caries initiation and progression is not well-understood. the distribution of actinomyces in supragingival plaque on the primary teeth and the relationship between oral actinomyces spp. and childhood caries primarily were investigated.200718306628
tyrosine sulfation of statherin.tyrosylprotein sulfotransferase (tpst), responsible for the sulfation of a variety of secretory and membrane proteins, has been identified and characterized in submandibular salivary glands (william et al. arch biochem biophys 1997; 338: 90-96). in the present study we demonstrate the sulfation of a salivary secretory protein, statherin, by the tyrosylprotein sulfotransferase present in human saliva. optimum statherin sulfation was observed at ph 6.5 and at 20 mm mncl(2). increase in the level o ...200717389930
in vitro activity of amoxicillin, clindamycin, doxycycline, metronidazole, and moxifloxacin against oral actinomyces.actinomyces spp have been increasingly associated with endodontic infections. however, the antimicrobial susceptibility of this genus has not been studied extensively. the objective of this study was to determine the susceptibility of oral isolates of actinomyces naeslundii, actinomyces gerencseriae, actinomyces israelii, actinomyces viscosus, and actinomyces odontolyticus to amoxicillin, clindamycin, doxycycline, metronidazole, and moxifloxacin using in vitro assays. the minimum inhibitory conc ...200717437871
antibacterial activity of four glass ionomer cements used in atraumatic restorative treatment.the in vitro antibacterial activity of four glass ionomer cements (fuji ix, ketac molar, vidrion r and vitromolar) indicated for atraumatic restorative treatment (art) was studied against strains of bacteria involved in the development of oral diseases, streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus and actinomyces viscosus. the agar plate diffusion test was used for the cultures, which included chlorhexidine as a positive control. the results demonstrated that all the c ...200717497079
[effects of the active compounds of m. officinalis on cariogenic bacteria].to investigate the effects of the principal biologically active compounds of m. officinalis on the growth and acid generation of the main cariogenic bacteria.200717593830
in vitro inhibition of bacterial growth using different dental adhesive systems.this study evaluated the antibacterial potential of four different adhesive systems.200717695612
[initial study on discrimination of oral microorganisms with the metabonomics technique].to evaluate the feasibility of employing metabonomics method in identification of oral pathogenic bacteria.200717896487
surface antibacterial properties of glass ionomer cements used in atraumatic restorative treatment.atraumatic restorative treatment (art) is recommended for use worldwide, not only in developing countries where resources are not readily available, but also in more industrialized countries. the antibacterial properties of restorative dental materials may improve the restorative treatment outcome. glass ionomer cement (gic) has been advocated as the preferred restoration material for art. the authors evaluated the antibacterial properties of restorative materials-three gics and a zinc oxide eug ...200717908849
in vitro anti-biofilm activity of macelignan isolated from myristica fragrans houtt. against oral primary colonizer bacteria.in early dental plaque formation, oral primary colonizers such as streptococcus mutans, streptococcus sanguis and actinomyces viscosus are initially attached to the pellicle-coated tooth surface to form a biofilm. the study aimed to determine the efficacy of macelignan, isolated from nutmeg (myristica fragrans houtt.), in removing each single oral primary biofilm in vitro on a polystyrene 96-well microtiter plate. four biofilm growth phases (4, 12, 20 and 24 h) were evaluated in this study after ...200817926328
dammarane- and taraxastane-type triterpenoids from saussurea oligantha franch.a new dammarane triterpenoid oliganthas a (1) and a new taraxastane triterpenoid oliganthas b (2), as well as five known taraxastane triterpenoids, ptiloepoxide (3), taraxast-20(30)ene-3beta,21alpha-diol (4), 22-oxo-20-taraxasten-3beta-ol (5), taraxast-20-ene-3beta,30-diol (6), and taraxastane-3beta,20alpha-diol (7), were isolated from the whole plant of saussurea oligantha franch. their structures were elucidated by a series of spectroscopic methods. these compounds, especially taraxastanes 2, ...200818464076
quercetin in a lotus leaves extract may be responsible for antibacterial activity.in the course of a search for chemotherapeutic agents inhibiting suspected peridontitis bacteria, extracted and purified substances from lotus leaf were identified by antimicrobial activity tests with use of the broth micro-dilution methods on 96-microwell plate. the antimicrobial activity of extracts was tested against five microorganisms, namely: actinobacillus actinomycetemcomitans y4, actinomyces viscosus 19246, porphyromonas gingivalis 33277, fusobacterium nucleatum 25586, and actinomyces n ...200818481022
antibacterial effects of three experimental quaternary ammonium salt (qas) monomers on bacteria associated with oral infections.the aim of this study was to test the antibacterial effects of three experimental quaternary ammonium salt monomers in order to evaluate their potential applications as dental materials. in vitro susceptibility testing of the monomers was performed by the broth dilution method on bacteria associated with oral infections: streptococcus mutans atcc 25175, actinomyces viscosus atcc 15987, staphylococcus aureus atcc 29213 and lactobacillus casei atcc 393. the time-kill kinetics of the monomer with r ...200818818469
evidence for recombination between a sialidase (nanh) of actinomyces naeslundii and actinomyces oris, previously named 'actinomyces naeslundii genospecies 1 and 2'.actinomyces spp., predominant members of human oral biofilms, may use extracellular sialidase to promote adhesion, deglycosylate immunoglobulins and liberation of nutrients. partial nanh gene sequences (1,077 bp) from actinomyces oris (n=74), actinomyces naeslundii (n=30), actinomyces viscosus (n=1) and actinomyces johnsonii (n=2) which included the active-site region and the bacterial neuraminidase repeats (bnrs) were compared. the sequences were aligned and each species formed a distinct clust ...200818823396
[study the inhibitory effects of three oral actinomyces on growth of oral candida albicans in vitro].the aim of this study was to investigate whether the three species of oral actinomyces have inhibitory effects on the growth of oral candida albicans in vitro.200819007084
effect of xylitol on an in vitro model of oral biofilm.the aim of the present study was to examine whether xylitol, at different concentrations, inhibits the formation of an experimental model of oral biofilm.200819178100
emended description of actinomyces naeslundii and descriptions of actinomyces oris sp. nov. and actinomyces johnsonii sp. nov., previously identified as actinomyces naeslundii genospecies 1, 2 and wva 963.actinomyces naeslundii is an important early colonizer in the oral biofilm and consists of three genospecies (1, 2 and wva 963) which cannot be readily differentiated using conventional phenotypic testing or on the basis of 16s rrna gene sequencing. we have investigated a representative collection of type and reference strains and clinical and oral isolates (n=115) and determined the partial gene sequences of six housekeeping genes (atpa, rpob, pgi, metg, glta and gyra). these sequences identifi ...200919244431
activity of panduratin a isolated from kaempferia pandurata roxb. against multi-species oral biofilms in vitro.the formation of dental biofilm caused by oral bacteria on tooth surfaces is the primary step leading to oral diseases. this study was performed to investigate the preventive and reducing effects of panduratin a, isolated from kaempferia pandurata roxb., against multi-species oral biofilms consisting of streptococcus mutans, streptococcus sanguis and actinomyces viscosus. minimum inhibitory concentration (mic) of panduratin a was determined by the clinical and laboratory standards institute (cls ...200919325204
dental caries vaccine.dental caries is one of the most common diseases in humans. in modern times, it has reached epidemic proportions. dental caries is an infectious microbiologic disease of the teeth that results in localized dissolution and destruction of the calcified tissue. dental caries is a mulitifactorial disease, which is caused by host, agent, and environmental factors. the time factor is important for the development and progression of dental caries. a wide group of microorganisms are identified from cari ...200919336869
the prevalence of multidose vial contamination by aerobic bacteria in a major teaching hospital, shiraz, iran, 2006.parenteral medications are usually given out in multidose vials (mdvs) and can be used for a prolonged period for 1 or more patients. the risk of extrinsic contamination of mdvs and its consequences may be serious and may lead to an outbreak, especially in hospitals. therefore, bacterial contamination of multiple-dose medication vials in namazi hospital, the major referral teaching hospital, in shiraz, southwestern iran, was evaluated.200919362388
microflora associated with successful and failed orthodontic mini-implants.mini-implants are used for orthodontic bone anchorage. the reasons for a potential instability or loss of the mini-implants during treatment are multiple. among other factors, colonization of implants with pathogenic bacteria is discussed. therefore, the microflora associated with successful and failed mini-implants has been screened.200919719743
comparative evaluation of antimicrobial activity of three cements: new endodontic cement (nec), mineral trioxide aggregate (mta) and portland.using the agar diffusion method, we conducted an in vitro study to evaluate the antimicrobial activity of mineral trioxide aggregate (mta), new endodontic cement (nec) and portland cement at different concentrations against five different microorganisms. a base layer was made using muller-hinton agar for escherichia coli (atcc 10538) and candida (atcc 10231). for actinomyces viscosus (atcc 15987), enterococcus faecalis (atcc 10541) and streptococcus mutans (atcc 25175) blood agar medium was used ...200919776512
intra-thoracic pyogranulomatous disease in four working dogs.case history: four working dogs were presented to veterinary clinics in new zealand for weight loss or swelling of the thoracic wall. all dogs were large-breed males between the ages of 4 and 7 years, and actively involved in farm work. the two dogs with swelling of the thoracic wall had recent histories of work-related trauma. clinical findings and diagnosis: all four dogs were diagnosed with intra-thoracic pyogranulomatous disease via diagnostic imaging and surgical exploration. two dogs had c ...200919966894
antimicrobial action of intracanal medicaments on the external root surface.to assess in vitro the antimicrobial action of intracanal medicaments in the external root surface, with or without of cementum.200918995944
an in vitro antimicrobial activity of ten iranian-made toothpastes.antimicrobial agents have been used as a chemotherapeutic agent to improve oral health. this in vitro study was carried out to determine the antimicrobial activity of ten iranian-made toothpastes against commonly found bacteria in the oral cavity.200921528037
evaluation of the antimicrobial, antioxidant, and anti-inflammatory activities of hydroxychavicol for its potential use as an oral care agent.hydroxychavicol isolated from the chloroform extraction of aqueous extract of piper betle leaves showed inhibitory activity against oral cavity pathogens. it exhibited an inhibitory effect on all of the oral cavity pathogens tested (mics of 62.5 to 500 microg/ml) with a minimal bactericidal concentration that was twofold greater than the inhibitory concentration. hydroxychavicol exhibited concentration-dependent killing of streptococcus mutans atcc 25175 up to 4x mic and also prevented the forma ...200918573934
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