Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the establishment of reproducible, complex communities of oral bacteria in the chemostat using defined inocula. | nine commonly isolated oral bacterial populations were inoculated into a glucose-limited and a glucose-excess (amino acid-limited) chemostat maintained at a constant ph 7.0 and a mean community generation time of 13.9 h. the bacterial populations were streptococcus mutans atcc 2-27351, strep. sanguis nctc 7865, strep. mitior ef 186, actinomyces viscosus wvu 627, lactobacillus casei ac 413, neisseria sp. a1078, veillonella alkalescens atcc 17745, bacteroides intermedius t 588 and fusobacterium nu ... | 1985 | 3932293 |
| [production of beta-d-glucosidase in a strain of actinomyces viscosus]. | 1985 | 3938835 | |
| 5-(alkylsulfonyl)salicylanilides as potential dental antiplaque agents. | a series of 22 5-(alkylsulfonyl)salicylanilides was synthesized and evaluated for in vitro antibacterial and antiplaque activity against actinomyces viscosus and streptococcus mutans, adherent microorganisms implicated in periodontal disease and dental caries. the minimum inhibitory concentrations of 25 salicylanilides (including 5-acyl-, 5-alkyl-, and 5-(alkylsulfonyl)-4'-bromo- and -4'-(trifluoromethyl)salicylanilides) were found to correlate (r = 0.94) with estimated log d values. several sal ... | 1986 | 3941411 |
| quantitative comparisons of potentially cariogenic microorganisms cultured from noncarious and carious root and coronal tooth surfaces. | potentially cariogenic microorganisms cultured from noncarious and carious root and coronal (enamel) surfaces were quantitatively compared in patients 22 to 84 years of age (mean, 52 years). we collected 150 plaque specimens from 26 in situ teeth with initial root lesions and from 25 extracted teeth with advanced root lesions. the frequencies of isolation of streptococcus mutans, actinomyces viscosus, and lactobacillus spp. were, respectively, 94, 72, and 51% at the noncarious root site; 98, 71, ... | 1986 | 3949379 |
| surface structures (peritrichous fibrils and tufts of fibrils) found on streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera. | we screened 36 strains of streptococcus sanguis biotype i and 8 strains of s. sanguis biotype ii for the presence of surface structures and for their ability to coaggregate with actinomyces viscosus, actinomyces naeslundii, and fusobacterium nucleatum. negative staining under an electron microscope revealed detectable surface structures on all s. sanguis strains. the majority of strains (38 of 44) carried peritrichous fibrils, which have an irregular profile and no distinct width. they usually a ... | 1985 | 3965396 |
| effect of local and parenteral immunization on implantation of actinomyces viscosus t6 in rats. | groups of rats immunized in the vicinity of the major salivary glands or immunized intraperitoneally with actinomyces viscosus t6 and their sham-immunized controls were infected with the homologous bacterium. significantly higher levels of salivary and serum antibody were induced by intraperitoneal than by salivary gland immunization. there were also significant inverse correlations between the levels of salivary and serum antibody and the levels of implantation of a. viscosus t6. the level of i ... | 1985 | 3965403 |
| relative hydrophobicities of actinomyces viscosus and actinomyces naeslundii strains and their adsorption to saliva-treated hydroxyapatite. | the present study examined 42 strains of actinomyces spp. to determine whether adsorption to saliva-treated hydroxyapatite (sha) of the selected strains of this prominent group of dental-plaque bacteria correlated with hydrophobicity. the relative hydrophobicity of the strains was determined by their adsorption to hydrophobic gels (i.e., phenyl-sepharose) and their aggregation in ammonium sulfate. within serogroups the relative hydrophobicity for the strains was similar. the relative adsorption ... | 1985 | 3972451 |
| simultaneous loss of bacteriophage receptor and coaggregation mediator activities in actinomyces viscosus mg-1. | actinomyces bacteriophages were used as tools to study coaggregation between actinomyces and streptococci. four bacteriophage isolates, phages av-1, av-2, av-3, and 1281, bound to coaggregation group a actinomyces viscosus and to group e a. naeslundii. no binding to groups b, c, d, or f was observed. only a. viscosus mg-1 was capable of supporting a productive infection by these phages. spontaneously occurring bacteriophage-resistant mutants of a. viscosus mg-1 were isolated and were shown to fa ... | 1985 | 3980085 |
| effects of peptidoglycans from periodontal pathogens on selected biological activities of cd-1 murine peritoneal macrophages. | resident cd-1 murine peritoneal macrophages were exposed to various concentrations of purified peptidoglycan isolated from members of the genera bacteroides, eikenella, and actinomyces. macrophage viability, the release of lysozyme, acid phosphatase, and prostaglandins e1 and e2 were assayed as a function of peptidoglycan concentration and time. macrophages responded as a function of peptidoglycan concentration with increased release of acid phosphatase and prostaglandins; all cells remained gre ... | 1985 | 3986715 |
| isolation of actinomyces bacteriophage from human dental plaque. | human dental plaque samples were screened for the presence of bacteriophage for actinomyces viscosus and streptococcus sanguis. none of the 336 samples yielded phage for s. sanguis, but 10 contained virulent actinomyces phage. a high host cell specificity was observed in that one phage isolate infected only a. viscosus t14v, eight phage isolates infected only a. viscosus mg-1, and one infected both strains. none was capable of productively infecting various other actinomyces strains that represe ... | 1985 | 4008044 |
| effect of saccharin on growth and acid production of glucose-grown pathogenic and oral bacteria. | growth and acid production of glucose-grown gram-positive and gram-negative rods as well as cocci from the human oral cavity were studied in the presence of 0.02 to 20.00 mg/ml sodium saccharin. all gram-positive rods, i.e. actinomyces viscosus, lactobacillus acidophilus, bacillus subtilis and corynebacterium diphtheriae, and gram-positive cocci, i.e. streptococcus spp, staphylococcus aureus and micrococcus luteus, were significantly inhibited by saccharin, especially at the higher concentration ... | 1985 | 4033457 |
| actinomyces viscosus cell-free synthesis of extracellular slime polysaccharide. | a cell-free extract of actinomyces viscosus t14av catalyzed the synthesis of extracellular n-acetylglucosamine-rich slime polysaccharide. the activity was localized in the cytoplasmic membrane fraction and required the presence of adp-glucose and udp-n-acetylglucosamine. maximal activity was demonstrated at ph 7.5 and also required the presence of divalent cations such as mg2+ or mn2+. extracellular slime appeared to serve as a primer for slime biosynthesis. the antibiotic tunicamycin acted as a ... | 1985 | 4046889 |
| langmuir and scatchard parameters do not describe the binding of actinomyces viscosus to saliva-treated hydroxyapatite. | the binding of actinomyces viscosus t14v to saliva-treated spheroidal hydroxyapatite (sha) beads was studied. the association constant (k) and the total number of binding sites (n) obtained from the langmuir plots were in good agreement with those reported by other workers (approx. 3 x 10(-8) and 3 x 10(8), respectively). the values for n obtained from scatchard plots differed from those obtained from langmuir plots by factors of 10(6) or more. these results suggest that either these equations a ... | 1985 | 4067574 |
| actinomyces viscosus in man. | 1972 | 4110339 | |
| serological identification of rodent strains of actinomyces viscosus and their relationship to actinomyces of human origin. | 1972 | 4114782 | |
| identification of actinomyces, arachnia, bacterionema, rothia, and propionibacterium species by defined immunofluorescence. | fractionated fluorescein-isothiocyanate (fitc)-conjugated immunoglobulin g (dye-to-protein ratio <10), produced against whole cells of actinomyces spp., arachnia, bacterionena, rothia, and propionibacterium spp., give species-specific conjugates with controlled nonspecific staining reactions when appropriately diluted on the basis of their antibody content (10 mg/ml). using this standardization in immunofluorescence, serotype-specific conjugates are also available after dilution for all serotype ... | 1973 | 4123707 |
| ultrastructure of actinomyces viscosus and actinomyces naeslundii. | 1974 | 4132890 | |
| a slime-producing microorganism in dental plaque of rats, selected by glucose feeding. chemical composition of extracellular slime elaborated by actinomyces viscosus, strain nyl. | 1974 | 4276010 | |
| galactosyl-diglyceride from actinomyces viscosus. | 1974 | 4366087 | |
| regulation of lactate dehydrogenase activity in actinomyces viscosus by adenosine-5'-triphosphate. | 1974 | 4374177 | |
| reactions in the periodontium to continuous antigenic stimulation in sensitized gnotobiotic rats. | the purpose of our experiment was to evaluate the destructive potential of a strain of actinomyces viscosus on the periodontium of sensitized rodents, describing the induced lesions on the basis of quantitative cytology. the experimental design comprised in principle the following procedures. young germfree rats were immunized either by intravenous or intradermal injections with heat-killed cells of a. viscosus ny 1 or sham immunized intradermally with physiological saline. after a period suitab ... | 1974 | 4426700 |
| canine actinomycosis due to actinomyces viscosus: report of six cases. | 1974 | 4496897 | |
| chemical and antigenic properties of the cell wall of actinomyces viscosus (strain t6). | 1972 | 4506556 | |
| [dental caries in the gnotobiotic rat inoculated with actinomyces viscosus and actinomyces naeslundii]. | 1972 | 4506978 | |
| plaque formation and dental caries in gnotobiotic and spf osborne-mendel rats associated with actinomyces viscosus. | 1974 | 4525551 | |
| chemical structure of the cell wall peptidoglycan of actinomyces viscosus. | 1974 | 4531862 | |
| actinomyces viscosus as an agent of actinomycosis in the dog. | 1972 | 4557020 | |
| numerical taxonomy and laboratory identification of bacterionema matruchotii, rothia dentocariosa, actinomyces naeslundii, actinomyces viscosus, and some related bacteria. | 1973 | 4579125 | |
| degradation of sucrose by whole cells and plaque of actinomyces naeslundii. | whole cells of actinomyces naeslundii atcc 12104, either in a dispersed form or in the form of plaque, enzymatically degraded sucrose to glucose and fructose. washed whole cells expressed beta-fructofuranosidase specificity and hydrolyzed sucrose to essentially equimolar quantities of glucose and fructose. the cells readily hydrolyzed sucrose, raffinose, and actinomyces viscosus or aerobacter levanicum levan, but did not degrade melezitose, maltose, alpha-methyl-d-glucoside, melibiose, glucose-1 ... | 1974 | 4611924 |
| method for preclinical evaluation of antiplaque agents. | a preclinical method for the evaluation of antibacterial agents for use against dental plaques associated with caries and periodontal disease is proposed. the method is applicable to screening agents and to defining, in vitro, the minimal conditions required for maximal antiplaque effect. as a model of antiplaque agents, chlorhexidine was assessed in vitro against preformed plaques of microorganisms conducive to dental caries and periodontal disease. the agent was bactericidal to plaques of nine ... | 1972 | 4670477 |
| actinomyces viscosus in a branchial cyst. | 1973 | 4751713 | |
| extracellular polysaccharides of actinomyces viscosus. | polysaccharide(s) have been isolated from supernatants of broth cultures of actinomyces viscosus, strain t6. the organism produces significant quantities (2 mg/mg of cell [dry weight], 108 mg/liter) of this polysaccharide in the absence of sucrose. chemical analysis indicated that n-acetylglucosamine (62%) was the major component; galactose (7%), glucose (4%), uronic acid (3%), and smaller amounts of glycerol, rhamnose, arabinose, and xylose were also present. the polysaccharide(s) is only sligh ... | 1974 | 4851986 |
| identification of human strains of actinomyces viscosus. | catalase-positive actinomycetes which closely resemble the "hamster organism" described by howell have been isolated from dental calculus and other human sources. these cultures could not be distinguished from the hamster strains on the basis of morphology, oxygen requirements, biochemical reactions, or cell wall composition. these human isolates have been classified with the hamster strains as actinomyces viscosus. the strains from hamster and human sources fell into two serotypes. serotype 1 c ... | 1969 | 4896106 |
| a cariogenic actinomyces viscosus--a bacteriological and gnotobiotic study. | 1971 | 5117164 | |
| production of an extracellular levan by odontomyces viscosus. | 1967 | 5227886 | |
| plaque formation and implantation of odontomyces viscosus in hamsters fed different carbohydrates. | 1969 | 5259424 | |
| levan formation by odontomyces viscosus. | 1969 | 5261310 | |
| [odontomyces viscosus, gen. nov. spec. nov]. | 1970 | 5274251 | |
| toxic properties of the cell wall of gram-positive bacteria. | the biological activity of odontomyces viscosus, which has been reported to cause periodontal disease in hamsters, was examined. the microorganism was cultured anaerobically in brain heart infusion broth, and the cells were harvested. the washed cells were injected intradermally into the abdomen of rabbits. after 72 hr, a well-defined, firm, raised nodule (about 1.0 by 1.5 cm) with an erythematous border was seen at the injection site. suspensions of cell wall and cytoplasmic material were injec ... | 1967 | 5335900 |
| odontomyces viscosus, gen. nov., spec. nov., a filamentous microorganism isolated from periodontal plaque in hamsters. | 1965 | 5896131 | |
| characterization of a monoclonal antibody specific for lipoteichoic acid from various gram-positive bacteria. | a hybrid cell line, 3g6, producing monoclonal antibody (mab) against the polyglycerophosphate (pgp) backbone of lipoteichoic acids has been derived by the polyethylene glycol-induced fusion of mouse myeloma cells and spleen cells from mice immunized with partially purified glucosyltransferase from culture supernatant of streptococcus mutans strain 6715. immunodiffusion tests and elisa revealed that the antibody reacted with purified pgp from group a streptococcus pyogenes strain sv as well as cr ... | 1984 | 6083437 |
| the use of monoclonal antibodies in the study of lactose-sensitive adherence of actinomyces viscosus t14v. | 1980 | 6108369 | |
| detection and localization of a lectin on actinomyces viscosus t14v by monoclonal antibodies. | a cell-associated lectin activity that mediates lactose-inhibitable adherence of actinomyces viscosus t14v has been localized to a specific population of fimbriae by the use of monoclonal antibodies. nine monoclonal antibodies were produced that reacted with only 1 of 2 immunoelectrophoretically distinct fimbrial components on t14v. the fibrillar morphology of this component was revealed by the immunoelectronmicroscopic examination of bacteria incubated with the monoclonal antibodies. the lectin ... | 1981 | 6115881 |
| antibodies against the ag2 fimbriae of actinomyces viscosus t14v inhibit lactose-sensitive bacterial adherence. | monospecific antisera against the ag1 and ag2 fimbrial components of actinomyces viscosus t14v were produced by immunizing rabbits with immune precipitates of each antigen harvested from crossed-immunoelectrophoresis plates. the fab fragments prepared from these sera were used as specific reagents in immunoelectron microscopy to identify each fimbrial component on the bacterial surface and also were assayed for their abilities to prevent the coaggregation of a. viscosus t14v with streptococcus s ... | 1982 | 6124506 |
| humoral immune response to oral microorganisms in periodontitis. | serum antibody titers from patients with periodontitis were compared with those from periodontally healthy subjects. with the micro-enzyme-linked immunosorbent assay, immunoglobulin g (igg), iga, and igm antibody titers to isolates of streptococcus sanguis, actinomyces viscosus, bacteroides gingivalis, bacteroides melaninogenicus subsp. intermedius, bacteroides gingivalis, bacteroides melaninogenicus subsp. intermedius, bacteroides ochraceus, and fusobacterium nucleation were determined. antibod ... | 1982 | 6126434 |
| specific absence of type 2 fimbriae on a coaggregation-defective mutant of actinomyces viscosus t14v. | the coaggregation-defective (cog-) mutant actinomyces viscosus t14v(pk455), which is unable to participate in lactose-sensitive adherence, and its cog+ parent were compared to structurally define the mutational loss of cell-associated lectin activity. immunoelectrophoretic comparisons of crude extracts or isolated fimbriae from both strains demonstrated type 2 fimbriae (previously designated ag2) in preparations obtained from the parent but none in those obtained from the mutant. this result was ... | 1983 | 6132880 |
| specific inhibition of adsorption of actinomyces viscosus t14v to saliva-treated hydroxyapatite by antibody against type 1 fimbriae. | specific antibodies and their fab fragments were used to study adsorption of antinomyces viscosus t14v to saliva-treated hydroxyapatite. antibody against a. viscosus t14v blocked adsorption of the strain to saliva-treated hydroxyapatite, and this activity was removed after preincubation of the immune immunoglobulin g (igg) with purified type 1 fimbriae in amounts near equivalence but not after preincubation with type 2 fimbriae in antigen excess. specific igg or fab fragments against type 1 fimb ... | 1984 | 6141143 |
| the function and distribution of different fimbriae on strains of actinomyces viscosus and actinomyces naeslundii. | actinomyces viscosus and actinomyces naeslundii differ in their abilities to colonize tooth and epithelial surfaces. these differences appear to be associated with the distribution of different fimbriae on the two species and with the distinct adherence-related functions of these structures. | 1984 | 6142065 |
| exclusive presence of lactose-sensitive fimbriae on a typical strain (wvu45) of actinomyces naeslundii. | lactose-sensitive fimbriae were identified as the only fimbriae present on actinomyces naeslundii wvu45 (atcc 12104). a single antigen reactive with antiserum against wvu45 cells was detected by cross immunoelectrophoresis of isolated fimbriae, and a monospecific antiserum against this antigen reacted with all fimbriae observed on the bacterial surface by immunoelectron microscopy. moreover, the loss of one cell surface antigen by a spontaneous mutant of a. naeslundii wvu45 (wvu45m), isolated by ... | 1984 | 6150007 |
| lectin-dependent attachment of actinomyces naeslundii to receptors on epithelial cells. | the adherence of actinomyces naeslundii wvu45 to monolayer cultures of human epithelial cell lines was mediated by the lactose-sensitive fimbriae (type 2) of strain wvu45. the attachment of actinomyces viscosus t14v, which has both types 1 and 2 fimbriae, was approximately half that of a. naeslundii, and only minimal attachment of a. naeslundii and a. viscosus mutants lacking type 2 fimbriae was detected. the adherence of strain wvu45 was enhanced two- to threefold by neuraminidase treatment of ... | 1984 | 6150008 |
| the absence of binding sites for antigens of actinomyces viscosus on rat fibroblasts. | 1981 | 6165814 | |
| characterization of a galactose-specific lectin from actinomyces viscosus by a model aggregation system. | a simple model system has been developed in which lectin-mediated aggregation of glycoprotein-coated beads can be monitored by following the decrease in light scattering at 650 nm. aggregation has been characterized with the lectin of actinomyces viscosus t14v. its dependence on ph, temperature, and stirring rate was examined, and the number of bacterial cells in relation to the number of latex beads resulting in optimal aggregation was established. this system has the advantage of permitting th ... | 1982 | 6185429 |
| chemical and immunological comparison of surface fibrils of strains representing six taxonomic groups of actinomyces viscosus and actinomyces naeslundii. | human isolates of actinomyces viscosus and actinomyces naeslundii have been divided into six clusters in a numerical taxonomy study. surface fibrils of strains representing these clusters were isolated and purified. chemical analyses revealed that the major component of all fibrils was protein and that although differences in percentages of specific amino acid residues were found, the relative proportions of basic, acidic, polar uncharged, and nonpolar amino acids were rather similar among clust ... | 1983 | 6188696 |
| automated immunofluorescent speciation of oral bacteria using flow cytometry. | mixtures containing two bacterial species were analyzed using flow cytometric techniques. light scattering characteristics of streptococcus mutans and actinomyces viscosus show unique profiles for pure cultures. however, the light scatter analysis of a mixture containing these two species demonstrates overlapping near the origin. thus, light scatter analysis was not sufficient to speciate bacteria with different morphologies. labeling of samples with species-specific immunofluorescent antibodies ... | 1984 | 6205030 |
| immunoelectron microscopic studies of actinomyces viscosus. | 1982 | 6211536 | |
| infrequent polysaccharide cohesion among actinomyces viscosus and actinomyces naeslundii fresh isolates of human origin. | 1983 | 6222174 | |
| degradation of the microbial and salivary components participating in human dental plaque formation by proteases elaborated by plaque bacteria. | twenty-eight strains of facultative, gram-positive, sporulating bacilli which produce caseinolytic enzymes were isolated from human early dental plaque. a major component of the extracellular caseinolytic enzymes elaborated by strong producers seemed to be neutral zinc proteases. the extracellular proteases inactivated glucosyltransferase of streptococcus mutans and inhibited the synthesis of adherent glucans from sucrose. the enzymes also degraded the strep. mutans cell-surface receptor for dex ... | 1983 | 6222727 |
| polymorphonuclear leukocyte regulation of lymphocyte proliferation and differentiation. | the polymorphonuclear neutrophil (pmn) regulates in vivo and in vitro immune responses. we report that the immunoenhancing properties of pmn culture supernatants from pmn recruited by the bacterium actinomyces viscosus (av) show its exclusive effects on the t cell lymphocyte population. a study of the effect of pmn supernatants on normal balb/c splenocytes to t and b cell mitogens showed enhancing effects on t cell mitogens, but no effect on b cell mitogen responses when compared to a control. a ... | 1983 | 6229470 |
| effects of the association of conventional rats with actinomyces viscosus nyl and bacteroides gingivalis w83. | 1984 | 6241229 | |
| influence of sublethal antibiotic concentrations on bacterial adherence to saliva-treated hydroxyapatite. | the influence of growth in the presence of sublethal concentrations of nine antibiotics on the ability of certain potentially odontopathic bacteria to attach to saliva-treated hydroxyapatite surfaces which mimic teeth was studied. cells of actinomyces viscosus ly7 and s2, bacteroides gingivalis 381, capnocytophaga ochraceus 6, and actinobacillus actinomycetemcomitans n27 attached in lower numbers to saliva-treated hydroxyapatite when grown in the presence of 50% of the minimum inhibitory concent ... | 1982 | 6274799 |
| enzymatic modification of bacterial receptors on saliva-treated hydroxyapatite surfaces. | certain properties of experimental pellicles formed by the adsorption of salivary components on hydroxyapatite surfaces change over time. to determine whether enzymes likely to be present in the oral environment could induce such changes, pellicles were treated with saliva which had been incubated for 18 h at 35 degrees c to promote the elaboration of microbial enzymes. this treatment markedly reduced the numbers of streptococcus mutans mt3 and jbp and s. sanguis fc-1 and c5 cells which attached ... | 1982 | 6281193 |
| a factor from actinomyces viscosus t14v that specifically aggregates streptococcus sanguis h1. | a highly specific aggregation factor for streptococcus sanguis h1 (afh1) was obtained by lysozyme treatment of actinomyces viscosus t14v. at 1 micrograms/ml, afh1 aggregated a suspension of s. sanguis h1, with which a. viscosus t14v coaggregates by a mechanism not inhibited by lactose: even at much higher levels afh1 caused little or no aggregation of streptococci from other coaggregation groups (j. o. cisar et al., infect. immun. 24:742-752, 1979). the most active fraction of afh1 obtained by g ... | 1983 | 6303957 |
| kinetics of adherence of actinomyces viscosus to saliva-coated silica and hydroxyapatite beads. | adherence of 14c-labelled strains of actinomyces viscosus to uncoated and saliva-coated silica and hydroxyapatite beads had both loose and firm components, probably reflecting different subpopulations of bacteria within a single culture. adherence was characterized by the proportion of bacteria available for each type of adherence and a constant (kb) for each combination of bacterial strain and bead surface. loose adherence, which was greater with silica than with hydroxyapatite beads, always in ... | 1983 | 6311943 |
| [effect of solid substrates of microbial colony development]. | bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: bacillus subtilis, b. cereus, streptococcus sanguis, s. saprophyticus, s. faecalis, escherichia coli, micrococcus flavus, actinomyces viscosus, veillonella sp., enterobacter liquefaciens, yersinia enterocolitica. lactobacillus casei behaved n ... | 1984 | 6326398 |
| of human dental plaque bacteria, actinomyces viscosus accelerates immunoglobulin a protease secretion in streptococcus sanguis. | 1983 | 6347146 | |
| [immunoglobulin a protease secretion in streptococcus sanguis accelerated by actinomyces viscosus]. | 1983 | 6361182 | |
| activation of the alternate complement pathway by peptidoglycan of actinomyces viscosus, a potentially pathogenic oral bacterium. | peptidoglycans and cells walls from actinomyces viscosus, staphylococcus aureus, and group a streptococcus were compared for their relative abilities to activate the alternate complement pathway (acp). on the dry-weight basis, the peptidoglycan from a. viscosus was 3.5 times more active than group a streptococcal peptidoglycan and 15.6 times more active than staph. aureus peptidoglycan in activating the acp. consequently a. viscosus peptidoglycan is one of the most potent acp-activators reported ... | 1983 | 6362630 |
| effect of saliva on coaggregation of oral actinomyces and streptococcus species. | human oral actinomyces and streptococci that exhibit specific coaggregation patterns when the cells are suspended in buffer were tested for their ability to coaggregate in saliva. of 53 paired combinations of actinomyces (actinomyces viscosus, a. naeslundii, or actinomyces sp. wva 963) and streptococci (streptococcus sanguis or s. morbillorum) that exhibited coaggregation in buffer, all but 4 pairs also coaggregated when suspended in saliva. twenty-four pairs exhibited lactose-inhibited coaggreg ... | 1984 | 6370861 |
| sucrase and maltase activities in supragingival dental plaque in humans of streptococcal, actinomyces and lactobacilli species. | 20 reference strains and 72 isolated strains from dental plaque of streptococci, actinomyces, and lactobacilli species were examined for sucrase and maltase activities. the type of sucrase in the different strains was determined by use of the alpha-glucosidase inhibitor, acarbose. the enzyme activities were determined as formation of monosaccharide, and quantitated spectrophotometrically. although variations occurred in enzyme activities between reference and isolated strains, the same general p ... | 1984 | 6372064 |
| identification of bacteria in immunopathologic mechanisms of human dental pulp. | serial frozen sections from human dental pulp were used for the identification of oral bacteria in immunopathologic mechanisms. sera raised against actinomyces viscosus, a. naeslundii, bacteroides gingivalis, and b. melaninogenicus ss. intermedius, commercial sera against human immunoglobulins, complement, and monoclonal antibodies against human t cells were used in a double-staining immunofluorescence technique. sections of dental pulp from normal teeth showed no penetration of bacteria or bact ... | 1984 | 6377171 |
| prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes. | fresh oral isolates from human dental plaque were selected on the basis of their spherical morphology. in a double-blind experiment, their species identity and ability to coaggregate with human oral actinomyces viscosus and actinomyces naeslundii were determined. of the 110 isolates characterized, 30 were identified as either streptococcus mutans, streptococcus anginosus-constellatus, or veillonella parvula; none of these coaggregated with the actinomycetes. the remaining 80 isolates were identi ... | 1983 | 6409806 |
| new actinomyces and streptococcus coaggregation groups among human oral isolates from the same site. | the coaggregation properties of recent human oral streptococcal and actinomyces isolates from the same site were determined and compared with the coaggregation properties of well-characterized stock strains of these two kinds of bacteria. streptococcus sanguis, actinomyces viscosus, actinomyces naeslundii, and phenotypically similar strains of actinomyces were isolated from subgingival samples from periodontally healthy older individuals, from persons participating in an experimental gingivitis ... | 1983 | 6409807 |
| interbacterial adherence between actinomyces viscosus and strains of streptococcus pyogenes, streptococcus agalactiae, and pseudomonas aeruginosa. | interbacterial adherence was sought between strains of actinomyces viscosus indigenous to the human mouth and strains of streptococcus pyogenes, streptococcus agalactiae, and pseudomonas aeruginosa. six of nine strains of s. pyogenes, three of five strains of s. agalactiae, and two of four strains of p. aeruginosa were found to coaggregate with each of five strains of a. viscosus tested. some coaggregation reactions were inhibited by 0.05 m lactose and were dependent upon heat- and protease-sens ... | 1984 | 6423545 |
| the effect of cyclosporin a on periodontal disease in rats monoassociated with actinomyces viscosus ny 1. | 1981 | 6453970 | |
| development of immunological sensitization and alveolar bone loss in gnotobiotic rats infected with actinomyces viscosus ny 1. | 1981 | 6453978 | |
| effects of fluoride, lithium and strontium on extracellular polysaccharide production by streptococcus mutans and actinomyces viscosus. | 1981 | 6455453 | |
| a possible mechanism for the cellular coaggregation between actinomyces viscosus atcc 19246 and streptococcus sanguis atcc 10557. | the cells of actinomyces viscosus atcc 19246 (av19246) and streptococcus sanguis atcc 10557 (ss10557) coaggregated immediately after mixing in 40 mm-tris/hcl buffer. optimal conditions were ph 7.5 in the presence of ca2+ at 0.1 mm or higher. na2 edta and its analogues, na2mgedta and na2mnedta at 7.5 mm inhibited the coaggregation. trypsin and heat treatment impaired the reactive site on av19246 cells, but not on ss10557 cells. the coaggregates, once formed, dissociated gradually during extended ... | 1984 | 6481336 |
| [bacteriological characterization of gingivitis-inducing plaque depending on different sugar levels of the diet]. | in a time period of 21 days an experimental gingivitis was induced in 8 volunteers. four volunteers received a carbohydrate-reduced, the other 4 persons a carbohydrate-enriched diet. clinical investigations on the development of plaque and gingivitis and bacteriological examinations of the supragingival microflora were performed simultaneously. the change in the composition of the supragingival plaque-microflora were nearly independent of the amount of food-carbohydrate. in the beginning of the ... | 1984 | 6485636 |
| isolation of wolinella recta and actinomyces viscosus from an actinomycotic chest wall mass. | in this note we describe a patient with an actinomycotic chest wall mass from which large numbers of wolinella recta and actinomyces viscosus organisms were isolated. this is the first reported association of w. recta with extraoral infection and the tenth report of lung infection by a. viscosus. | 1984 | 6520225 |
| in vitro activity of a-16686, a potential antiplaque agent. | a-16686, a new glycoproteide antibiotic from actinoplanes sp., was evaluated as a potential antiplaque agent in comparison with chlorhexidine, benzalkonium chloride, and cetylpyridinium chloride. a-16686 had good activity against gram-positive organisms associated with dental plaque (various streptococci, streptococcus mutans in particular, lactobacilli, actinomyces viscosus, and actinomyces naeslundii); most of the strains tested were clinical isolates. it was bactericidal for streptococci (mbc ... | 1984 | 6549119 |
| isolation and characterization of intracellular polysaccharide from actinomyces viscosus. | 1983 | 6571808 | |
| sodium fluoride susceptibilities of suspected periodontopathic bacteria. | utilizing a blood-based medium, we determined the minimum inhibitory concentrations (mic's) to sodium fluoride (naf) of 45 bacterial strains representing 22 oral species. bacterial susceptibility ranged from 128 micrograms/ml to 2048 micrograms/ml. of those organisms tested, actinobacillus actinomycetemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga ochracea, and actinomyces viscosus were susceptible at lower levels of naf. | 1983 | 6574154 |
| colonization of the teeth of rats by human and rodent oral strains of the bacterium actinomyces viscosus. | the mouths of rats were infected with human and rodent strains of a. viscosus and streptomycin-rifamycin-resistant (sr) mutants of these strains. the establishment, adherence to the teeth and cell dose required for infection were determined. human and rodent strains established equally well on the teeth and did not differ in their initial adherence to teeth. the cell dose required for infection was much lower for rodent than for human strains (less than 10(5) compared to 10(7) - 10(8) c.f.u.). t ... | 1983 | 6574735 |
| effect of factors other than pathologic status on responsiveness of peripheral blood mononuclear cells from patients with chronic periodontitis. | studies were designed to assess factors other than pathologic status of the cell donor which affect the blastogenic responsiveness in vitro of peripheral blood mononuclear cells (pbms) from normal donors and patients with periodontitis. cultures were established and activated using phytohemagglutinin-p (pha) or homogenates of actinomyces viscosus (avis), a gram-positive plaque microorganism, and fusobacterium nucleatum (fuso), a gram-negative plaque microorganism. activation was assessed by meas ... | 1983 | 6577178 |
| actinomyces viscosus and actinomyces naeslundii agglutinins in human saliva. | the objectives were to determine the degree of actinomyces agglutinating activity in human saliva and to begin characterizing the agglutination mechanism. agglutination titres of whole saliva collected from adults and 6-yr-old children were compared. titres for a. naeslundii were always higher than for a. viscosus. the mean a. naeslundii titre for the adults' and children's samples were equivalent. the children had a slightly lower mean titre than the adults for a. viscosus. no correlation was f ... | 1983 | 6579605 |
| predominant cultivable microflora of plaque on removable dentures in patients with healthy oral mucosa. | plaque from the fitting surface of upper full dentures in eight patients with healthy palatal mucosa was studied. to characterize the predominant cultivable flora, 916 isolates (100-128 from each sample) were subcultured from anaerobic roll-tubes. streptococci constituted 0-81 per cent (median, 41 per cent) of the isolates with varying proportions of streptococcus milleri, streptoccus mutans, streptococcus salivarius, streptococcus mitior and streptococcus sanguis. staphylococcus aureus made up ... | 1983 | 6579900 |
| use of bacteriophage-resistant mutants to study actinomyces viscosus cell surface receptors. | spontaneously occurring bacteriophage-resistant mutants of actinomyces viscosus were isolated. the mutants exhibited altered coaggregation patterns with streptococci. from analysis of the data, it appears that a cell surface structure on a. viscosus may function both as a phage receptor and as a binding site for co-aggregation with certain oral streptococci. these mutants will be valuable in the study of surface structures that mediate one type of the cell-to-cell interactions that occur between ... | 1983 | 6580320 |
| tetracycline and its derivatives strongly bind to and are released from the tooth surface in active form. | several antibiotics were found to adsorb to saliva-coated enamel and to inhibit in vitro plaque formation by pure cultures of oral bacteria: actinomyces viscosus, actinomyces naeslundii and streptococcus mutans. tetracycline, minocycline and oxytetracycline adsorbed to the greatest degree, showing 100-fold higher adsorption than spiramycin, the test antibiotics with least adsorption. inhibition of in vitro plaque formation was found to require both drug substantivity (capacity for adsorption) an ... | 1983 | 6580410 |
| inhibition of lactose-reversible adherence between actinomyces viscosus and oral streptococci by salivary components. | 1984 | 6584210 | |
| the effect of chlorhexidine on the colonization of the human and rat dentition by actinomyces viscosus. | 1984 | 6584211 | |
| human serum precipitins to oral bacteria related to dental caries. | adults aged 20-38 years were classified by a positive (+) or negative (-) precipitating serum antibody response to d-alanyl glycerol teichoic acid (s), or an antigen from actinomyces spp. (a). culture filtrates of streptococcus mutans and actinomyces viscosus were used to detect the respective specific antibodies by immunoelectrophoresis and immunodiffusion when present in serum at a concentration of more than 0.05 mg/ml. among subjects who had grown up in fluoridated areas, a+ subjects exhibite ... | 1984 | 6587838 |
| the effects of mutual interaction and host diet on the growth rates of the bacteria actinomyces viscosus and streptococcus mutans during colonization of tooth surfaces in di-associated gnotobiotic rats. | the bacteria were simultaneously inoculated into the mouths of germ-free rats. the maximum growth rates attained by strep. mutans (doubling time td = 1.4 h) or a. viscosus (td = 2.7 h) were approximately the same as in mono-associated gnotobiotic rats [td = 1.1 and 2.8 h, respectively (beckers and van der hoeven, 1982a)]. the presence of glucose or sucrose did not affect the maximum growth rates. in starved rats, the accumulation of microorganisms tended to decline sooner, suggesting that the gr ... | 1984 | 6587844 |
| the pattern of experimental colonization of a human and a rodent strain of the bacterium actinomyces viscosus on the dentition of the rat. | samples were taken from mesial, buccal, lingual and approximal sites and from fissures. initially, most a. viscosus were recovered from the retention sites. with the exception of lingual sites during the period of exponential growth, the apparent doubling times calculated for a. viscosus nyl sr remained within narrow limits for all locations. after cessation of exponentional growth, both strains had colonized all surfaces. however, the rodent strain a. viscosus nyl sr had formed 30-800 times lar ... | 1984 | 6588932 |
| effects of fluoride, lithium, and strontium on intracellular polysaccharide accumulation in s. mutans and a. viscosus. | fluoride (5 mg/l) consistently depressed the accumulation of intracellular iodophilic polysaccharides (ips) in streptococcus mutans strains bht, fa-1, and gs-5 by over 90% and in actinomyces viscosus strain rc-45 by over 50%. there was little further reduction in ips content when fluoride was increased from 5 to 100 mg/l. lithium (0 to 1 mg/l) neither enhanced nor inhibited ips accumulation, nor did it modify the inhibitory effects of fluoride in three of the four strains tested. strontium (0 to ... | 1984 | 6589274 |
| human neutrophil migration under agarose to bacteria associated with the development of gingivitis. | in clinically healthy gingiva and increasingly with the development of inflammation, neutrophils are found in the gingival tissues and sulcus. this study evaluated the relative ability of bacteria associated with gingival health and developing inflammation to stimulate this increase in neutrophil accumulation. dialyzed bacterial sonic extracts (be) in buffer and pooled human serum (phs) from pure cultures of streptococcus sanguis. actinomyces viscosus, a naeslundii, bacteroides intermedius, fuso ... | 1984 | 6592328 |
| growth and acid tolerance of human dental plaque bacteria. | pure cultures of representative strains of cariogenic and non-cariogenic plaque bacteria were assessed for their ability to initiate and maintain growth in broths, adjusted to initial ph levels of 7.0, 5.5 or 5.0, and to produce lactic acid from sucrose or glucose in resting-cell suspensions at ph 6.5, 5.0, 4.5 and 4.0. streptococcus mutans, lactobacillus casei and streptococcus faecalis showed greater acid tolerance than strains of streptococcus sanguis, streptococcus salivarius, streptococcus ... | 1984 | 6594096 |
| a simple method for extracting human lymphocyte stimulants from human dental plaque bacteria of the genus actinomyces. | the effect of acid concentration on the hot-acid extraction of antigens from actinomyces viscosus that stimulate human peripheral blood lymphocytes in vitro was tested. the optimum ph for release of these antigens in a soluble low molecular-weight form was ph 2.3. preliminary purification on sepharose 4b demonstrated that most of the lympho-stimulatory material (about 90 per cent) had a mol. wt of about 100,000. this partially-purified antigenic material was mainly protein. the ph 2.3 hot-acid e ... | 1984 | 6594104 |
| potential cariogenicity of lycasin 80/55 before and after repeated transmissions of the dental plaque flora in rats. | five successive experiments, with rats fed ad libitum on diets containing sucrose or lycasin 80/55, were carried out. in experiment i, the rats were inoculated with streptococcus mutans alone or with strep. mutans in combination with actinomyces viscosus. in three successive transmission experiments (ii, iii, iv), the rats were inoculated with plaque of the rats of the preceding experiment. the rats of experiment v were inoculated with the original strains or with plaque derived from experiment ... | 1984 | 6598369 |
| nonspecific induction of immunoglobulin m antibodies to periodontal disease-associated microorganisms after polyclonal human b-lymphocyte activation by fusobacterium nucleatum. | the production of antibodies to oral bacteria was determined in lymphocyte cultures stimulated with sonicated fusobacterium nucleatum, a potent inducer of polyclonal b-cell activation. after 9 days the cultures were examined by a microenzyme-linked immunosorbent assay for immunoglobulin m (igm) antibodies to f. nucleatum, bacteroides gingivalis, actinomyces viscosus, and streptococcus sanguis. antibodies to these four bacteria were detected in cultures stimulated with polyclonal b-cell activatio ... | 1983 | 6604024 |
| mitogen-induced hyperproliferation response of peripheral blood mononuclear cells from patients with severe generalized periodontitis: lack of correlation with proportions of t cells and t-cell subsets. | severe generalized periodontitis (sgp) is a localized inflammatory disease which differs clinically from common periodontitis in that it leads to remarkable extensive alveolar bone loss in relatively young adults. there is evidence that b-cell responses to bacterial substances may play a major role in the pathogenesis of this disease. in the present report, we show that a b-cell mitogen from actinomyces viscosus (avis) bacteria provokes a hyperproliferation response of peripheral blood mononucle ... | 1984 | 6607802 |