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effect of microbial interaction on the colonization rate of actinomyces viscosus or streptococcus mutans in the dental plaque of rats.the resident oral microflora of conventional osborne-mendel rats was challenged with actinomyces viscosus or streptococcus mutans strains. the adherence of the inoculated organism to the tooth surface and the subsequent growth were studied by means of viable counts determination. the initial growth rate of s. mutans in conventional rats was lower than in mono-associated gnotobiotic rats (doubling time, td = 5 h versus td = 1.1 h). the delayed start of growth and the low initial growth rate indic ...19827141698
glucose uptake by streptococcus mutans, streptococcus mitis, and actinomyces viscosus in the presence of human saliva.glucose uptake was examined by using whole-cell suspensions of streptococcus mutans (strains bht, ingbritt, and gs-5), streptococcus mitis (strains 9811 and 72x41), and actinomyces viscosus (strains t6 and wvu626) incubated for up to 90 min in 0 to 82% (vol/vol) human whole salivary supernatant. glucose uptake by the s. mutans strains was completely inhibited at all saliva concentrations. dithiothreitol (dtt), present during saliva incubation, prevented saliva inhibition. glucose uptake was also ...19827152663
of human dental plaque bacteria, actinomyces viscosus accelerates immunoglobulin a protease secretion in streptococcus sanguis. 19836347146
specific coaggregation and the cell wall of streptococcus sanguis.sacculi prepared from streptococcus sanguis 34 by extensive extraction of cells with hot sodium dodecyl sulfate-2-mercaptoethanol retained the ability to coaggregate with actinomyces viscosus t14v. when s. sanguis 34 was disrupted by homogenization with glass beads and fractionated by differential centrifugation, only the cell wall fraction agglutinated a. viscosus t14v. when strain 34 was treated with lysozyme, the coaggregating capability of the cells was essentially unaltered. sacculi prepare ...19836874647
comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles.oral bacteria were found to differ in their surface hydrophobicities as determined by their ability to adsorb to hexadecane. strains of actinomyces viscosus, a. naeslundii, streptococcus sanguis, s. mitis, and bacteroides gingivalis proved highly hydrophobic. strains of b. intermedius, s. salivarius, s. mutans, and b. melaninogenicus were less hydrophobic, whereas strains of actinobacillus actinomycetemcomitans were hydrophilic. an overall correlation was noted between the adsorption of bacteria ...19836885158
copper-inhibition of the growth of oral streptococci and actinomyces.copper ions were found to inhibit the rate of growth in broth culture of streptococcus mitis, streptococcus mutans, streptococcus salivarious, streptococcus sanguis, actinomyces viscosus and actinomyces naeslundii. in all cases, 10(-3) m copper inhibited the rate of growth, whereas 10(-4) m and lower concentrations had little or no effect. at the concentrations used in mouthwashes one mode of action of copper ions is to reduce the rate of growth of oral bacteria in vitro.19836615987
carbohydrate metabolism by actinomyces viscosus growing in continuous culture.a human oral strain of actinomyces viscosus, gn431/75, was grown anaerobically in a defined medium in continuous culture with a glucose limitation at dilution rates (d) between 0.025 and 0.2 h-1 and with a nitrogen limitation at d = 0.005 and 0.1 h-1. with 5 mg of glucose per ml, the culture was limited for carbon at d = 0.025 and 0.05 h-1, but became nitrogen limited (asparagine) at d = 0.1 and 0.2 h-1. the molar growth yield (yglucose) decreased from 50.0 to 40.9 g of cells per mol of glucose ...19836618664
[immunoglobulin a protease secretion in streptococcus sanguis accelerated by actinomyces viscosus]. 19836361182
further studies on the growth inhibition of some oral bacteria by xylitol.the growth in brain heart infusion medium containing glucose or sucrose of streptococcus mutans strain omz 176, two strains of strep. sanguis (atcc 10556 and no. 39) and actinomyces viscosus type atcc 27044 was inhibited by xylitol but not by d-arabitol or ribitol. when fructose was substituted for glucose, xylitol had no growth-inhibiting effect. it was also shown that the bacteria produced less acid in the presence of xylitol, as measured by the ph of the cultures or by neutralization with alk ...19836624463
[isolation of actinomyces viscosus from an abscess caused by a dog bite]. 19836672934
comparative histopathology of lesions produced by actinomyces israelii, actinomyces naeslundii, and actinomyces viscosus in mice.the histopathologic features of experimental actinomycotic lesions produced in mice by actinomyces israelii, actinomyces naeslundii, and actinomyces viscosus were examined. in lesions caused by a israelii the outer edge of the bacterial granule exhibited an eosinophilic fringe with no evidence of penetration of polymorphonuclear leukocytes (pmns) into the bacterial granule. chronic lesions after 6 weeks contained lobulated advancing fronts as well as areas of resolution showing heavy penetration ...19836829706
chemical characterization of extracellular polysaccharides produced by actinomyces viscosus t14v and t14av.the human isolates actinomyces viscosus t14v and t14av produced extracellular polysaccharides in the absence of sucrose. in contrast to strain t14v, strain t14av produced abundant viscous slime polysaccharide in the culture supernatant fluids when grown in a chemically defined medium containing glucose. after resolution of the t14av polysaccharides into seven fractions, it was demonstrated that two of these exhibited viscous properties and lacked methylpentose and muramic acid. the major slime p ...19836840834
influence of physicochemical parameters on adsorption of actinomyces viscosus to hydroxyapatite surfaces.adsorption of actinomyces viscosus strains t14v and t14av to saliva-treated and untreated hydroxyapatite (ha) under various environmental conditions was studied. strain t14v adsorption to saliva-treated ha was not influenced by ph, whereas strain t14av adsorbed in higher numbers under acidic conditions. the addition of cations inhibited adsorption of strains t14v and t14av to saliva-treated ha. strain t14v possessed a greater affinity for hydrophobic gels than did strain t14av, but incorporation ...19836840836
in vitro antiplaque activity of octenidine dihydrochloride (win 41464-2) against preformed plaques of selected oral plaque-forming microorganisms.the antibacterial activity of octenidine dihydrochloride (win 41464-2) against intact preformed in vitro plaques of four indigenous oral plaque-forming microorganisms, streptococcus mutans, streptococcus sanguis, actinomyces viscosus, and actinomyces naeslundii, was studied. both absolute (plaque bactericidal index) and relative (chlorhexidine coefficient) indices of antiplaque efficacy were established. octenidine dihydrochloride compared favorably with chlorhexidine digluconate with respect to ...19836847170
common antigens of streptococcal and nonstreptococcal oral bacteria: characterization of wall-associated protein and comparison with extracellular protein antigen.a soluble wall-associated common protein (wap) has been isolated from cell wall-extracted antigens of exponentially growing cells of streptococcus sanguis omz9, two serotypes (a and e), of streptococcus mutans and actinomyces viscosus omz104. the wap antigens from the different strains were obtained by chromatography on an anti-rabbit immunoglobulin column of sodium dodecyl sulfate-dissociated immunoprecipitates. the affinity-isolated wap antigens from the different oral bacteria were identical, ...19836852918
binding of 18f by cell membranes and cell walls of streptococcus mutans.the binding of 18f to isolated cell membranes and cell walls of streptococcus mutans gs-5 or other bacteria was assayed. the attachment of 18f to these cell envelopes proceeded slowly and reached equilibrium within 60 min. 18f binding was stimulated by ca2+ (1 mm). the binding of 18f to cellular components was dependent upon the ph, as well as the amount of 18f and dose of the binder employed. the binding of 18f by cell walls prepared from fluoride-sensitive and fluoride-resistant cells of s. sa ...19836862629
structural preferences of beta-galactoside-reactive lectins on actinomyces viscosus t14v and actinomyces naeslundii wvu45.specificities of lectins on actinomyces viscosus t14v and actinomyces naeslundii wvu45 were compared by measuring the abilities of d-galactose, n-acetyl-d-galactosamine, 14 beta-d-galacto-oligosaccharides, and 2 beta-d-fuco-oligosaccharides to inhibit coaggregation between streptococcus sanguis 34 and each actinomycete. inhibition profiles were similar, but wvu45 was significantly more sensitive to several inhibitors. d-galactose-beta(1 leads to 3)-n-acetyl-d-galactosamine glycosides were most p ...19836874075
isolation and characterization of intracellular polysaccharide from actinomyces viscosus. 19836571808
a factor from actinomyces viscosus t14v that specifically aggregates streptococcus sanguis h1.a highly specific aggregation factor for streptococcus sanguis h1 (afh1) was obtained by lysozyme treatment of actinomyces viscosus t14v. at 1 micrograms/ml, afh1 aggregated a suspension of s. sanguis h1, with which a. viscosus t14v coaggregates by a mechanism not inhibited by lactose: even at much higher levels afh1 caused little or no aggregation of streptococci from other coaggregation groups (j. o. cisar et al., infect. immun. 24:742-752, 1979). the most active fraction of afh1 obtained by g ...19836303957
colonization of the teeth of rats by human and rodent oral strains of the bacterium actinomyces viscosus.the mouths of rats were infected with human and rodent strains of a. viscosus and streptomycin-rifamycin-resistant (sr) mutants of these strains. the establishment, adherence to the teeth and cell dose required for infection were determined. human and rodent strains established equally well on the teeth and did not differ in their initial adherence to teeth. the cell dose required for infection was much lower for rodent than for human strains (less than 10(5) compared to 10(7) - 10(8) c.f.u.). t ...19836574735
actinomyces viscosus and actinomyces naeslundii agglutinins in human saliva.the objectives were to determine the degree of actinomyces agglutinating activity in human saliva and to begin characterizing the agglutination mechanism. agglutination titres of whole saliva collected from adults and 6-yr-old children were compared. titres for a. naeslundii were always higher than for a. viscosus. the mean a. naeslundii titre for the adults' and children's samples were equivalent. the children had a slightly lower mean titre than the adults for a. viscosus. no correlation was f ...19836579605
nonspecific induction of immunoglobulin m antibodies to periodontal disease-associated microorganisms after polyclonal human b-lymphocyte activation by fusobacterium nucleatum.the production of antibodies to oral bacteria was determined in lymphocyte cultures stimulated with sonicated fusobacterium nucleatum, a potent inducer of polyclonal b-cell activation. after 9 days the cultures were examined by a microenzyme-linked immunosorbent assay for immunoglobulin m (igm) antibodies to f. nucleatum, bacteroides gingivalis, actinomyces viscosus, and streptococcus sanguis. antibodies to these four bacteria were detected in cultures stimulated with polyclonal b-cell activatio ...19836604024
predominant cultivable microflora of plaque on removable dentures in patients with healthy oral mucosa.plaque from the fitting surface of upper full dentures in eight patients with healthy palatal mucosa was studied. to characterize the predominant cultivable flora, 916 isolates (100-128 from each sample) were subcultured from anaerobic roll-tubes. streptococci constituted 0-81 per cent (median, 41 per cent) of the isolates with varying proportions of streptococcus milleri, streptoccus mutans, streptococcus salivarius, streptococcus mitior and streptococcus sanguis. staphylococcus aureus made up ...19836579900
use of bacteriophage-resistant mutants to study actinomyces viscosus cell surface receptors.spontaneously occurring bacteriophage-resistant mutants of actinomyces viscosus were isolated. the mutants exhibited altered coaggregation patterns with streptococci. from analysis of the data, it appears that a cell surface structure on a. viscosus may function both as a phage receptor and as a binding site for co-aggregation with certain oral streptococci. these mutants will be valuable in the study of surface structures that mediate one type of the cell-to-cell interactions that occur between ...19836580320
tetracycline and its derivatives strongly bind to and are released from the tooth surface in active form.several antibiotics were found to adsorb to saliva-coated enamel and to inhibit in vitro plaque formation by pure cultures of oral bacteria: actinomyces viscosus, actinomyces naeslundii and streptococcus mutans. tetracycline, minocycline and oxytetracycline adsorbed to the greatest degree, showing 100-fold higher adsorption than spiramycin, the test antibiotics with least adsorption. inhibition of in vitro plaque formation was found to require both drug substantivity (capacity for adsorption) an ...19836580410
prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes.fresh oral isolates from human dental plaque were selected on the basis of their spherical morphology. in a double-blind experiment, their species identity and ability to coaggregate with human oral actinomyces viscosus and actinomyces naeslundii were determined. of the 110 isolates characterized, 30 were identified as either streptococcus mutans, streptococcus anginosus-constellatus, or veillonella parvula; none of these coaggregated with the actinomycetes. the remaining 80 isolates were identi ...19836409806
new actinomyces and streptococcus coaggregation groups among human oral isolates from the same site.the coaggregation properties of recent human oral streptococcal and actinomyces isolates from the same site were determined and compared with the coaggregation properties of well-characterized stock strains of these two kinds of bacteria. streptococcus sanguis, actinomyces viscosus, actinomyces naeslundii, and phenotypically similar strains of actinomyces were isolated from subgingival samples from periodontally healthy older individuals, from persons participating in an experimental gingivitis ...19836409807
production, purification, and properties of a bacteriocin from staphylococcus aureus isolated from saliva.staphylococci from samples of human saliva were isolated on staphylococcal-selective agar plates. these strains were tested for the inhibition of the growth of staphylococcus aureus fda209p. the frequency of inhibitory strains among all of the staphylococcal isolates was 5.2%. strain iys2, which formed the biggest inhibitory zone against the growth of the indicator strain, was used as the producer of bacteriocin. iys2 was identified to be s. aureus, based on its biological properties. the bacter ...19836832811
activation of the alternate complement pathway by peptidoglycan of actinomyces viscosus, a potentially pathogenic oral bacterium.peptidoglycans and cells walls from actinomyces viscosus, staphylococcus aureus, and group a streptococcus were compared for their relative abilities to activate the alternate complement pathway (acp). on the dry-weight basis, the peptidoglycan from a. viscosus was 3.5 times more active than group a streptococcal peptidoglycan and 15.6 times more active than staph. aureus peptidoglycan in activating the acp. consequently a. viscosus peptidoglycan is one of the most potent acp-activators reported ...19836362630
infrequent polysaccharide cohesion among actinomyces viscosus and actinomyces naeslundii fresh isolates of human origin. 19836222174
degradation of the microbial and salivary components participating in human dental plaque formation by proteases elaborated by plaque bacteria.twenty-eight strains of facultative, gram-positive, sporulating bacilli which produce caseinolytic enzymes were isolated from human early dental plaque. a major component of the extracellular caseinolytic enzymes elaborated by strong producers seemed to be neutral zinc proteases. the extracellular proteases inactivated glucosyltransferase of streptococcus mutans and inhibited the synthesis of adherent glucans from sucrose. the enzymes also degraded the strep. mutans cell-surface receptor for dex ...19836222727
polymorphonuclear leukocyte regulation of lymphocyte proliferation and differentiation.the polymorphonuclear neutrophil (pmn) regulates in vivo and in vitro immune responses. we report that the immunoenhancing properties of pmn culture supernatants from pmn recruited by the bacterium actinomyces viscosus (av) show its exclusive effects on the t cell lymphocyte population. a study of the effect of pmn supernatants on normal balb/c splenocytes to t and b cell mitogens showed enhancing effects on t cell mitogens, but no effect on b cell mitogen responses when compared to a control. a ...19836229470
sodium fluoride susceptibilities of suspected periodontopathic bacteria.utilizing a blood-based medium, we determined the minimum inhibitory concentrations (mic's) to sodium fluoride (naf) of 45 bacterial strains representing 22 oral species. bacterial susceptibility ranged from 128 micrograms/ml to 2048 micrograms/ml. of those organisms tested, actinobacillus actinomycetemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga ochracea, and actinomyces viscosus were susceptible at lower levels of naf.19836574154
kinetics of adherence of actinomyces viscosus to saliva-coated silica and hydroxyapatite beads.adherence of 14c-labelled strains of actinomyces viscosus to uncoated and saliva-coated silica and hydroxyapatite beads had both loose and firm components, probably reflecting different subpopulations of bacteria within a single culture. adherence was characterized by the proportion of bacteria available for each type of adherence and a constant (kb) for each combination of bacterial strain and bead surface. loose adherence, which was greater with silica than with hydroxyapatite beads, always in ...19836311943
effect of factors other than pathologic status on responsiveness of peripheral blood mononuclear cells from patients with chronic periodontitis.studies were designed to assess factors other than pathologic status of the cell donor which affect the blastogenic responsiveness in vitro of peripheral blood mononuclear cells (pbms) from normal donors and patients with periodontitis. cultures were established and activated using phytohemagglutinin-p (pha) or homogenates of actinomyces viscosus (avis), a gram-positive plaque microorganism, and fusobacterium nucleatum (fuso), a gram-negative plaque microorganism. activation was assessed by meas ...19836577178
chemical and immunological comparison of surface fibrils of strains representing six taxonomic groups of actinomyces viscosus and actinomyces naeslundii.human isolates of actinomyces viscosus and actinomyces naeslundii have been divided into six clusters in a numerical taxonomy study. surface fibrils of strains representing these clusters were isolated and purified. chemical analyses revealed that the major component of all fibrils was protein and that although differences in percentages of specific amino acid residues were found, the relative proportions of basic, acidic, polar uncharged, and nonpolar amino acids were rather similar among clust ...19836188696
specific absence of type 2 fimbriae on a coaggregation-defective mutant of actinomyces viscosus t14v.the coaggregation-defective (cog-) mutant actinomyces viscosus t14v(pk455), which is unable to participate in lactose-sensitive adherence, and its cog+ parent were compared to structurally define the mutational loss of cell-associated lectin activity. immunoelectrophoretic comparisons of crude extracts or isolated fimbriae from both strains demonstrated type 2 fimbriae (previously designated ag2) in preparations obtained from the parent but none in those obtained from the mutant. this result was ...19836132880
specific inhibition of adsorption of actinomyces viscosus t14v to saliva-treated hydroxyapatite by antibody against type 1 fimbriae.specific antibodies and their fab fragments were used to study adsorption of antinomyces viscosus t14v to saliva-treated hydroxyapatite. antibody against a. viscosus t14v blocked adsorption of the strain to saliva-treated hydroxyapatite, and this activity was removed after preincubation of the immune immunoglobulin g (igg) with purified type 1 fimbriae in amounts near equivalence but not after preincubation with type 2 fimbriae in antigen excess. specific igg or fab fragments against type 1 fimb ...19846141143
the function and distribution of different fimbriae on strains of actinomyces viscosus and actinomyces naeslundii.actinomyces viscosus and actinomyces naeslundii differ in their abilities to colonize tooth and epithelial surfaces. these differences appear to be associated with the distribution of different fimbriae on the two species and with the distinct adherence-related functions of these structures.19846142065
exclusive presence of lactose-sensitive fimbriae on a typical strain (wvu45) of actinomyces naeslundii.lactose-sensitive fimbriae were identified as the only fimbriae present on actinomyces naeslundii wvu45 (atcc 12104). a single antigen reactive with antiserum against wvu45 cells was detected by cross immunoelectrophoresis of isolated fimbriae, and a monospecific antiserum against this antigen reacted with all fimbriae observed on the bacterial surface by immunoelectron microscopy. moreover, the loss of one cell surface antigen by a spontaneous mutant of a. naeslundii wvu45 (wvu45m), isolated by ...19846150007
lectin-dependent attachment of actinomyces naeslundii to receptors on epithelial cells.the adherence of actinomyces naeslundii wvu45 to monolayer cultures of human epithelial cell lines was mediated by the lactose-sensitive fimbriae (type 2) of strain wvu45. the attachment of actinomyces viscosus t14v, which has both types 1 and 2 fimbriae, was approximately half that of a. naeslundii, and only minimal attachment of a. naeslundii and a. viscosus mutants lacking type 2 fimbriae was detected. the adherence of strain wvu45 was enhanced two- to threefold by neuraminidase treatment of ...19846150008
[effect of solid substrates of microbial colony development].bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: bacillus subtilis, b. cereus, streptococcus sanguis, s. saprophyticus, s. faecalis, escherichia coli, micrococcus flavus, actinomyces viscosus, veillonella sp., enterobacter liquefaciens, yersinia enterocolitica. lactobacillus casei behaved n ...19846326398
a possible mechanism for the cellular coaggregation between actinomyces viscosus atcc 19246 and streptococcus sanguis atcc 10557.the cells of actinomyces viscosus atcc 19246 (av19246) and streptococcus sanguis atcc 10557 (ss10557) coaggregated immediately after mixing in 40 mm-tris/hcl buffer. optimal conditions were ph 7.5 in the presence of ca2+ at 0.1 mm or higher. na2 edta and its analogues, na2mgedta and na2mnedta at 7.5 mm inhibited the coaggregation. trypsin and heat treatment impaired the reactive site on av19246 cells, but not on ss10557 cells. the coaggregates, once formed, dissociated gradually during extended ...19846481336
[bacteriological characterization of gingivitis-inducing plaque depending on different sugar levels of the diet].in a time period of 21 days an experimental gingivitis was induced in 8 volunteers. four volunteers received a carbohydrate-reduced, the other 4 persons a carbohydrate-enriched diet. clinical investigations on the development of plaque and gingivitis and bacteriological examinations of the supragingival microflora were performed simultaneously. the change in the composition of the supragingival plaque-microflora were nearly independent of the amount of food-carbohydrate. in the beginning of the ...19846485636
effects of the association of conventional rats with actinomyces viscosus nyl and bacteroides gingivalis w83. 19846241229
in vitro activity of a-16686, a potential antiplaque agent.a-16686, a new glycoproteide antibiotic from actinoplanes sp., was evaluated as a potential antiplaque agent in comparison with chlorhexidine, benzalkonium chloride, and cetylpyridinium chloride. a-16686 had good activity against gram-positive organisms associated with dental plaque (various streptococci, streptococcus mutans in particular, lactobacilli, actinomyces viscosus, and actinomyces naeslundii); most of the strains tested were clinical isolates. it was bactericidal for streptococci (mbc ...19846549119
automated immunofluorescent speciation of oral bacteria using flow cytometry.mixtures containing two bacterial species were analyzed using flow cytometric techniques. light scattering characteristics of streptococcus mutans and actinomyces viscosus show unique profiles for pure cultures. however, the light scatter analysis of a mixture containing these two species demonstrates overlapping near the origin. thus, light scatter analysis was not sufficient to speciate bacteria with different morphologies. labeling of samples with species-specific immunofluorescent antibodies ...19846205030
characterization of a monoclonal antibody specific for lipoteichoic acid from various gram-positive bacteria.a hybrid cell line, 3g6, producing monoclonal antibody (mab) against the polyglycerophosphate (pgp) backbone of lipoteichoic acids has been derived by the polyethylene glycol-induced fusion of mouse myeloma cells and spleen cells from mice immunized with partially purified glucosyltransferase from culture supernatant of streptococcus mutans strain 6715. immunodiffusion tests and elisa revealed that the antibody reacted with purified pgp from group a streptococcus pyogenes strain sv as well as cr ...19846083437
isolation of wolinella recta and actinomyces viscosus from an actinomycotic chest wall mass.in this note we describe a patient with an actinomycotic chest wall mass from which large numbers of wolinella recta and actinomyces viscosus organisms were isolated. this is the first reported association of w. recta with extraoral infection and the tenth report of lung infection by a. viscosus.19846520225
effects of fluoride, lithium, and strontium on intracellular polysaccharide accumulation in s. mutans and a. viscosus.fluoride (5 mg/l) consistently depressed the accumulation of intracellular iodophilic polysaccharides (ips) in streptococcus mutans strains bht, fa-1, and gs-5 by over 90% and in actinomyces viscosus strain rc-45 by over 50%. there was little further reduction in ips content when fluoride was increased from 5 to 100 mg/l. lithium (0 to 1 mg/l) neither enhanced nor inhibited ips accumulation, nor did it modify the inhibitory effects of fluoride in three of the four strains tested. strontium (0 to ...19846589274
human neutrophil migration under agarose to bacteria associated with the development of gingivitis.in clinically healthy gingiva and increasingly with the development of inflammation, neutrophils are found in the gingival tissues and sulcus. this study evaluated the relative ability of bacteria associated with gingival health and developing inflammation to stimulate this increase in neutrophil accumulation. dialyzed bacterial sonic extracts (be) in buffer and pooled human serum (phs) from pure cultures of streptococcus sanguis. actinomyces viscosus, a naeslundii, bacteroides intermedius, fuso ...19846592328
growth and acid tolerance of human dental plaque bacteria.pure cultures of representative strains of cariogenic and non-cariogenic plaque bacteria were assessed for their ability to initiate and maintain growth in broths, adjusted to initial ph levels of 7.0, 5.5 or 5.0, and to produce lactic acid from sucrose or glucose in resting-cell suspensions at ph 6.5, 5.0, 4.5 and 4.0. streptococcus mutans, lactobacillus casei and streptococcus faecalis showed greater acid tolerance than strains of streptococcus sanguis, streptococcus salivarius, streptococcus ...19846594096
a simple method for extracting human lymphocyte stimulants from human dental plaque bacteria of the genus actinomyces.the effect of acid concentration on the hot-acid extraction of antigens from actinomyces viscosus that stimulate human peripheral blood lymphocytes in vitro was tested. the optimum ph for release of these antigens in a soluble low molecular-weight form was ph 2.3. preliminary purification on sepharose 4b demonstrated that most of the lympho-stimulatory material (about 90 per cent) had a mol. wt of about 100,000. this partially-purified antigenic material was mainly protein. the ph 2.3 hot-acid e ...19846594104
mitogen-induced hyperproliferation response of peripheral blood mononuclear cells from patients with severe generalized periodontitis: lack of correlation with proportions of t cells and t-cell subsets.severe generalized periodontitis (sgp) is a localized inflammatory disease which differs clinically from common periodontitis in that it leads to remarkable extensive alveolar bone loss in relatively young adults. there is evidence that b-cell responses to bacterial substances may play a major role in the pathogenesis of this disease. in the present report, we show that a b-cell mitogen from actinomyces viscosus (avis) bacteria provokes a hyperproliferation response of peripheral blood mononucle ...19846607802
interbacterial adherence between actinomyces viscosus and strains of streptococcus pyogenes, streptococcus agalactiae, and pseudomonas aeruginosa.interbacterial adherence was sought between strains of actinomyces viscosus indigenous to the human mouth and strains of streptococcus pyogenes, streptococcus agalactiae, and pseudomonas aeruginosa. six of nine strains of s. pyogenes, three of five strains of s. agalactiae, and two of four strains of p. aeruginosa were found to coaggregate with each of five strains of a. viscosus tested. some coaggregation reactions were inhibited by 0.05 m lactose and were dependent upon heat- and protease-sens ...19846423545
the effect of chlorhexidine on the colonization of the human and rat dentition by actinomyces viscosus. 19846584211
human serum precipitins to oral bacteria related to dental caries.adults aged 20-38 years were classified by a positive (+) or negative (-) precipitating serum antibody response to d-alanyl glycerol teichoic acid (s), or an antigen from actinomyces spp. (a). culture filtrates of streptococcus mutans and actinomyces viscosus were used to detect the respective specific antibodies by immunoelectrophoresis and immunodiffusion when present in serum at a concentration of more than 0.05 mg/ml. among subjects who had grown up in fluoridated areas, a+ subjects exhibite ...19846587838
the effects of mutual interaction and host diet on the growth rates of the bacteria actinomyces viscosus and streptococcus mutans during colonization of tooth surfaces in di-associated gnotobiotic rats.the bacteria were simultaneously inoculated into the mouths of germ-free rats. the maximum growth rates attained by strep. mutans (doubling time td = 1.4 h) or a. viscosus (td = 2.7 h) were approximately the same as in mono-associated gnotobiotic rats [td = 1.1 and 2.8 h, respectively (beckers and van der hoeven, 1982a)]. the presence of glucose or sucrose did not affect the maximum growth rates. in starved rats, the accumulation of microorganisms tended to decline sooner, suggesting that the gr ...19846587844
the pattern of experimental colonization of a human and a rodent strain of the bacterium actinomyces viscosus on the dentition of the rat.samples were taken from mesial, buccal, lingual and approximal sites and from fissures. initially, most a. viscosus were recovered from the retention sites. with the exception of lingual sites during the period of exponential growth, the apparent doubling times calculated for a. viscosus nyl sr remained within narrow limits for all locations. after cessation of exponentional growth, both strains had colonized all surfaces. however, the rodent strain a. viscosus nyl sr had formed 30-800 times lar ...19846588932
inhibition of lactose-reversible adherence between actinomyces viscosus and oral streptococci by salivary components. 19846584210
effect of saliva on coaggregation of oral actinomyces and streptococcus species.human oral actinomyces and streptococci that exhibit specific coaggregation patterns when the cells are suspended in buffer were tested for their ability to coaggregate in saliva. of 53 paired combinations of actinomyces (actinomyces viscosus, a. naeslundii, or actinomyces sp. wva 963) and streptococci (streptococcus sanguis or s. morbillorum) that exhibited coaggregation in buffer, all but 4 pairs also coaggregated when suspended in saliva. twenty-four pairs exhibited lactose-inhibited coaggreg ...19846370861
osteomyelitis in dogs and cats caused by anaerobic bacteria.localised osteomyelitis was diagnosed in 2 dogs and 2 cats. the disease was caused by fight wounds in 3 cases. radiographic examination demonstrated a circumscribed zone of cortical bone lysis, sequestra and periosteal new bone. each case was treated surgically by sequestrectomy and debridement. infection was due mainly to anaerobic bacteria. the pathogenic bacteria isolated from the lesions of dogs were actinomyces viscosus, fusobacterium nucleatum and bacteroides spp, and from the lesions in c ...19846732670
sucrase and maltase activities in supragingival dental plaque in humans of streptococcal, actinomyces and lactobacilli species.20 reference strains and 72 isolated strains from dental plaque of streptococci, actinomyces, and lactobacilli species were examined for sucrase and maltase activities. the type of sucrase in the different strains was determined by use of the alpha-glucosidase inhibitor, acarbose. the enzyme activities were determined as formation of monosaccharide, and quantitated spectrophotometrically. although variations occurred in enzyme activities between reference and isolated strains, the same general p ...19846372064
identification of bacteria in immunopathologic mechanisms of human dental pulp.serial frozen sections from human dental pulp were used for the identification of oral bacteria in immunopathologic mechanisms. sera raised against actinomyces viscosus, a. naeslundii, bacteroides gingivalis, and b. melaninogenicus ss. intermedius, commercial sera against human immunoglobulins, complement, and monoclonal antibodies against human t cells were used in a double-staining immunofluorescence technique. sections of dental pulp from normal teeth showed no penetration of bacteria or bact ...19846377171
potential cariogenicity of lycasin 80/55 before and after repeated transmissions of the dental plaque flora in rats.five successive experiments, with rats fed ad libitum on diets containing sucrose or lycasin 80/55, were carried out. in experiment i, the rats were inoculated with streptococcus mutans alone or with strep. mutans in combination with actinomyces viscosus. in three successive transmission experiments (ii, iii, iv), the rats were inoculated with plaque of the rats of the preceding experiment. the rats of experiment v were inoculated with the original strains or with plaque derived from experiment ...19846598369
growth of oral streptococcus species and actinomyces viscosus in human saliva.microorganisms in dental plaque live in constant association with saliva. the role of saliva in the adherence of bacteria to the teeth and the antibacterial properties of saliva have been well investigated; less interest has been shown in the possible role of saliva as a substrate for oral microorganisms. in this study it was shown that saliva can serve as a growth medium for oral streptococcus spp. and actinomyces viscosus. the cell production of these organisms on saliva was carbohydrate limit ...19846742834
simultaneous loss of bacteriophage receptor and coaggregation mediator activities in actinomyces viscosus mg-1.actinomyces bacteriophages were used as tools to study coaggregation between actinomyces and streptococci. four bacteriophage isolates, phages av-1, av-2, av-3, and 1281, bound to coaggregation group a actinomyces viscosus and to group e a. naeslundii. no binding to groups b, c, d, or f was observed. only a. viscosus mg-1 was capable of supporting a productive infection by these phages. spontaneously occurring bacteriophage-resistant mutants of a. viscosus mg-1 were isolated and were shown to fa ...19853980085
fluctuations in crevicular and salivary anti-a. viscosus antibody levels in response to treatment of gingivitis.experimental gingivitis studies support a pathogenic rôle for actinomyces viscosus because of its numerical predominance in disease-associated plaques. the aims of the present investigation were to quantify specific crevicular igg against a. viscosus before and after conventional gingivitis treatment and to determine whether salivary iga and igg against a. viscosus and a. naeslundii would be affected concomitantly. 6 subjects with generalized gingivitis were selected. examinations were made befo ...19853902910
effects of peptidoglycans from periodontal pathogens on selected biological activities of cd-1 murine peritoneal macrophages.resident cd-1 murine peritoneal macrophages were exposed to various concentrations of purified peptidoglycan isolated from members of the genera bacteroides, eikenella, and actinomyces. macrophage viability, the release of lysozyme, acid phosphatase, and prostaglandins e1 and e2 were assayed as a function of peptidoglycan concentration and time. macrophages responded as a function of peptidoglycan concentration with increased release of acid phosphatase and prostaglandins; all cells remained gre ...19853986715
isolation of actinomyces bacteriophage from human dental plaque.human dental plaque samples were screened for the presence of bacteriophage for actinomyces viscosus and streptococcus sanguis. none of the 336 samples yielded phage for s. sanguis, but 10 contained virulent actinomyces phage. a high host cell specificity was observed in that one phage isolate infected only a. viscosus t14v, eight phage isolates infected only a. viscosus mg-1, and one infected both strains. none was capable of productively infecting various other actinomyces strains that represe ...19854008044
effect of local and parenteral immunization on implantation of actinomyces viscosus t6 in rats.groups of rats immunized in the vicinity of the major salivary glands or immunized intraperitoneally with actinomyces viscosus t6 and their sham-immunized controls were infected with the homologous bacterium. significantly higher levels of salivary and serum antibody were induced by intraperitoneal than by salivary gland immunization. there were also significant inverse correlations between the levels of salivary and serum antibody and the levels of implantation of a. viscosus t6. the level of i ...19853965403
characterization of protein and mannan polysaccharide antigens of yeasts, moulds, and actinomycetes.antigens in coccidioidin were compared with purified subfractions via tandem immunoelectrophoresis (iep) and by a combination of advancing line and crossed iep. rocket iep was suitable for titrating the reactions and showing the relationship between column fractions. these techniques required multicomponent antisera produced by hyperimmunization over many months and by the use of known standard migration pairs. the iep variations were used to chart the development of antisera against coccidioidi ...19853916766
tissue reactivity in the rat following administration of actinomyces viscosus in freund's adjuvant. 19853843524
lactate dehydrogenase from streptococcus mutans: purification, characterization, and crossed antigenicity with lactate dehydrogenases from lactobacillus casei, actinomyces viscosus, and streptococcus sanguis.a cytoplasmic fructose-1,6-diphosphate-dependent lactate dehydrogenase (ldh; ec 1.1.1.27) from streptococcus mutans omz175 was purified to homogeneity as judged by sodium dodecyl sulfate-gel electrophoresis. the purification consisted of ammonium sulfate precipitation of the cytoplasmic fraction, deae-sephacel and blue-sepharose cl.6b chromatography, and sephacryl s200 gel permeation. the catalytic activity of the purified enzyme required the presence of fructose-1,6-diphosphate with a broad opt ...19853917978
the different response of guinea pig peritoneal macrophages to various stimulators.to help clarify the mechanism of prostaglandin (pg) e2 and thromboxane (tx) b2 production by macrophages, the effects of three different stimulators (calcium ionophore a 23187, zymosan a and actinomyces viscosus) on the release of arachidonic acid and the production of pge2 and txb2 from guinea pig macrophages were examined. in the present study, we showed that the produced levels of pge2 and txb2 were not dependent on the release of arachidonic acid from the phospholipids.19853930344
[production of beta-d-glucosidase in a strain of actinomyces viscosus]. 19853938835
effect of saccharin on growth and acid production of glucose-grown pathogenic and oral bacteria.growth and acid production of glucose-grown gram-positive and gram-negative rods as well as cocci from the human oral cavity were studied in the presence of 0.02 to 20.00 mg/ml sodium saccharin. all gram-positive rods, i.e. actinomyces viscosus, lactobacillus acidophilus, bacillus subtilis and corynebacterium diphtheriae, and gram-positive cocci, i.e. streptococcus spp, staphylococcus aureus and micrococcus luteus, were significantly inhibited by saccharin, especially at the higher concentration ...19854033457
actinomyces viscosus cell-free synthesis of extracellular slime polysaccharide.a cell-free extract of actinomyces viscosus t14av catalyzed the synthesis of extracellular n-acetylglucosamine-rich slime polysaccharide. the activity was localized in the cytoplasmic membrane fraction and required the presence of adp-glucose and udp-n-acetylglucosamine. maximal activity was demonstrated at ph 7.5 and also required the presence of divalent cations such as mg2+ or mn2+. extracellular slime appeared to serve as a primer for slime biosynthesis. the antibiotic tunicamycin acted as a ...19854046889
langmuir and scatchard parameters do not describe the binding of actinomyces viscosus to saliva-treated hydroxyapatite.the binding of actinomyces viscosus t14v to saliva-treated spheroidal hydroxyapatite (sha) beads was studied. the association constant (k) and the total number of binding sites (n) obtained from the langmuir plots were in good agreement with those reported by other workers (approx. 3 x 10(-8) and 3 x 10(8), respectively). the values for n obtained from scatchard plots differed from those obtained from langmuir plots by factors of 10(6) or more. these results suggest that either these equations a ...19854067574
the association between dietary sucrose consumption and microbial population shifts at six oral sites in man.sucrose-related microbial population shifts were evaluated at 6 oral sites in 22 volunteers, who consumed high-sucrose diets for 21 days followed by low-sucrose diets for 21 days. culturing was performed at 0, 12, 21, 33 and 42 days of the 6-week experiment. over 50,000 microbial isolates were characterized and analysed. analysis of initial cultures showed the following site-specific microbial characteristics of the 6 sites evaluated: (1) molar fissures harboured higher levels of neisseria speci ...19853861144
relative hydrophobicities of actinomyces viscosus and actinomyces naeslundii strains and their adsorption to saliva-treated hydroxyapatite.the present study examined 42 strains of actinomyces spp. to determine whether adsorption to saliva-treated hydroxyapatite (sha) of the selected strains of this prominent group of dental-plaque bacteria correlated with hydrophobicity. the relative hydrophobicity of the strains was determined by their adsorption to hydrophobic gels (i.e., phenyl-sepharose) and their aggregation in ammonium sulfate. within serogroups the relative hydrophobicity for the strains was similar. the relative adsorption ...19853972451
establishment and distribution of the bacteria actinomyces viscosus and actinomyces naeslundii in the mouths of monkeys (macaca fascicularis).the colonization of the mouth by the two actinomyces species, postulated to be members of a basic plaque flora, was studied in 27 consecutively-born neonatal monkeys. bacteria adherent to the tongue and cheek surfaces were sampled from each monkey soon after birth (mean age = 3.2 days). the tongue and cheek surfaces and the labial surfaces of the incisor teeth were sampled at 4, 8 and 12 weeks of age. from only one neonatal sample were actinomyces species isolated. by 4 weeks of age, the incisor ...19853861145
phagocytosis of actinomyces viscosus by murine macrophages. susceptible and resistant strains. 19852935612
nonbactericidal approach to reduce colonization of plaque microflora on teeth in vitro and in vivo.a non-antibacterial, surface-modifying, perfluorosulfonamidoalkyl ester of phosphorous acid (psaep, 1) was adsorbed to saliva-coated hydroxyapatite disks in vitro. pretreatment of saliva-coated hydroxyapatite beads with different concentrations of 1 markedly reduced the adherence of radiolabeled streptococcus mutans or actinomyces viscosus when compared with the buffer-treated controls. pretreatment of the cells with the compound also significantly impaired their subsequent attachment to saliva- ...19853003336
bacterial profiles of subgingival plaques in periodontitis.in this report over 400 subgingival plaque samples taken from over 110 patients were examined microscopically and culturally for 30 bacterial parameters. the patients could be placed into six disease categories based upon clinical criteria. the bacterial profile of each clinical category was generally distinctive of that category. periodontal patients who had been successfully treated and maintained had plaques that were populated by significantly higher proportions of streptococcus sanguis, act ...19853869648
the establishment of reproducible, complex communities of oral bacteria in the chemostat using defined inocula.nine commonly isolated oral bacterial populations were inoculated into a glucose-limited and a glucose-excess (amino acid-limited) chemostat maintained at a constant ph 7.0 and a mean community generation time of 13.9 h. the bacterial populations were streptococcus mutans atcc 2-27351, strep. sanguis nctc 7865, strep. mitior ef 186, actinomyces viscosus wvu 627, lactobacillus casei ac 413, neisseria sp. a1078, veillonella alkalescens atcc 17745, bacteroides intermedius t 588 and fusobacterium nu ...19853932293
surface structures (peritrichous fibrils and tufts of fibrils) found on streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera.we screened 36 strains of streptococcus sanguis biotype i and 8 strains of s. sanguis biotype ii for the presence of surface structures and for their ability to coaggregate with actinomyces viscosus, actinomyces naeslundii, and fusobacterium nucleatum. negative staining under an electron microscope revealed detectable surface structures on all s. sanguis strains. the majority of strains (38 of 44) carried peritrichous fibrils, which have an irregular profile and no distinct width. they usually a ...19853965396
the mitogenicity for murine splenocytes of specific surface components of the oral periodontopathic bacterium, actinomyces viscosus.many characterized fractions obtained from a. viscosus were examined to identify the macromolecules responsible for mitogenicity for lymphocytes. spleen-cell suspensions of cba/j mice were cultured with 50-200 micrograms dry weight of a. viscosus strains t14v and t14av cellular components. lipopolysaccharide (lps) (escherichia coli) was used as a positive control. mechanical disruption with a french pressure cell or sonication produced preparations with a stimulation of 69,082 and 45,183 counts ...19853865642
the comparative cariogenicity and plaque-forming ability in vivo of four species of the bacterium actinomyces in gnotobiotic rats.gnotobiotic wag/rij rats on a high sucrose diet were monoinfected with 12 strains of actinomyces spp. moderate levels of caries were induced by a single strain of actinomyces naeslundii and low levels by two strains of actinomyces viscosus, three strains of a. naeslundii and one strain of actinomyces israelii. no caries was induced by single strains of a. viscosus and a. israelii or by three actinomyces odontolyticus strains. only fissure caries was observed. scanning electron microscopy showed ...19853868974
[experimental induction of periodontal disease in hamsters using actinomyces viscosus isolated from humans and a diet enriched with brown sugar]. 19853869869
[experimental production of periodontal disease in hamsters with actinomyces viscosus of human origin and a brown sugar-enriched diet]. 19853870020
peptidoglycan from the potentially pathogenic oral bacterium actinomyces viscosus is a b-cell mitogen.cell walls and peptidoglycan from actinomyces viscosus, strain m-100 were compared for their ability to act as mitogens with spleen cells from germ-free fischer rats. the cell walls were prepared from trypticase soy broth grown whole cells using a french press, followed by two consecutive washes with 0.1 m tris-hcl buffer, ph 8.0, 1 m nacl and distilled water. peptidoglycan was prepared from cell walls by three consecutive formamide extractions at 165 degrees c. on a dry-weight basis, the peptid ...19853873234
assignment of actinomyces viscosus and actinomyces naeslundii strains to numerical taxonomy clusters by immunofluorescence based on antifibril antisera.a previous observation, using a few representative laboratory strains, that rabbit antisera raised against isolated surface fibrils might be useful in identifying actinomyces viscosus and actinomyces naeslundii isolates to their correct numerical taxonomy clusters was reexamined with a large culture collection, including clinical isolates from three different laboratories. strains were first identified by a slower standard procedure, including agglutination with anti-whole-cell antisera, catalas ...19853889051
stimulation of human blood lymphocyte by different polyclonal b cell activators of bacterial and plant origin: production of igm, igg and iga estimated by the elisa method.lymphocytes isolated from peripheral blood of healthy donors were stimulated in vitro with pokeweed mitogen, concanavalin a, flagellin, nocardia delipidated cell mitogen (ndcm) and heat-killed bacteria escherichia coli and actinomyces viscosus. a simple and sensitive technique, enzyme-linked immunosorbent assay (elisa) was used for the detection of nanogram levels of igm, iga and igc in media from lymphocyte cultures after polyclonal stimulation, pokeweed mitogen, ndcm and e. coli were shown to ...19853891552
[polyclonal induction of igg antibody-forming cells (afc) by the stimulation of actinomyces viscosus t 14 v]. 19852411833
factors affecting binding of galacto ligands to actinomyces viscosus lectin.the specificity requirements for the binding of actinomyces viscosus t14v were examined by testing simple sugars, oligopeptides, and glycoproteins as inhibitors of the aggregation of glycoprotein-coated latex beads and washed a. viscosus cells. lactose was the most inhibitory simple sugar; d-fucose and d-galactose were equally inhibitory, methyl-alpha-d-fucoside was slightly less inhibitory, and l-fucose and raffinose were not inhibitory. the concentration of galactose residues required for 50% ...19852578122
immunoregulation in severe generalized periodontitis.severe generalized periodontitis (sgp) is an inflammatory disease which leads to extensive alveolar bone loss in young adults. peripheral blood lymphocytes from sgp patients have been previously reported to exhibit an in vitro hyperproliferative response when exposed to b cell mitogens derived from staphylococcus aureus and actinomyces viscosus. therefore hyperresponsiveness to b-cell mitogens could be an important pathogenic factor in the susceptibility to and progression of sgp. we have tested ...19852856901
expression of actinomyces viscosus antigens in escherichia coli: cloning of a structural gene (fima) for type 2 fimbriae.a cosmid gene library of actinomyces viscosus t14v was prepared in escherichia coli to examine the expression of a. viscosus antigens and to gain insight into the structure of a. viscosus type 1 and type 2 fimbriae. out of this library of 550 clones, 28 reacted in a colony immunoassay with antibodies against a. viscosus cells. the proteins responsible for these reactions were identified in three clones. clones av1209 and av2009 displayed nonfimbrial antigens with subunits of 40 and 58 kilodalton ...19852860095
ph change in artificial dental plaque formed by glucosyltransferase and some oral bacteria during batch and continuous culture.streptococcus mutans alone or cell-free glucosyltransferase (g-tase)-together with either streptococcus sanguis, streptococcus salivarius, actinomyces viscosus, or lactobacillus casei cells-formed artificial dental plaque that firmly adhered to glass electrodes in a continuous culture system containing sucrose. the ph in these artificial plaque samples decreased more than did that of the surrounding medium. in the absence of gtase, the bacteria other than s. mutans did not form firmly-adhering p ...19853156164
actinomyces adsorption mediated by type-1 fimbriae.monospecific antibody against the type-1 fimbriae of actinomyces viscosus t14v reacted with 12 strains of a. viscosus (serotype 2), 12 strains of a. naeslundii (serotype 2 or serotype 3), but not with 11 serotype 1 strains of a. naeslundii. all strains positive for the type-1 fimbrial antigen adsorbed strongly to saliva-treated hydroxyapatite, and in many instances (18 of 24 strains) this interaction was inhibited by the monospecific antibody. these findings strongly suggest that only those stra ...19862874164
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