| benzoic acid production with respect to starter culture and incubation temperature during yogurt fermentation using response surface methodology. | benzoic acid is occasionally used as a raw material supplement in food products and is sometimes generated during the fermentation process. in this study, the production of naturally occurring yogurt preservatives was investigated for various starter cultures and incubation temperatures, and considered food regulations. streptococcus thermophilus, lactobacillus acidophilus, lactobacillus delbrueckii subsp. bulgaricus, lactobacillus rhamnosus, lactobacillus casei, lactobacillus paracasei, lactoba ... | 2016 | 27433115 |
| brazilian kefir: structure, microbial communities and chemical composition. | microbial ecology and chemical composition of brazilian kefir beverage was performed. the microorganisms associated with brazilian kefir were investigated using a combination of phenotypic and genotypic methods. a total of 359 microbial isolates were identified. lactic acid bacteria (60.5%) were the major isolated group identified, followed by yeasts (30.6%) and acetic acid bacteria (8.9%). lactobacillus paracasei (89 isolates), lactobacillus parabuchneri (41 isolates), lactobacillus casei (32 i ... | 2011 | 24031681 |
| nutrition of the critically ill - emphasis on liver and pancreas. | about 25 million individuals undergo high risk surgery each year. of these about 3 million will never return home from hospital, and the quality of life for many of those who return is often significantly impaired. furthermore, many of those who manage to leave hospital have undergone severe life-threatening complications, mostly infections/sepsis. the development is strongly associated with the level of systemic inflammation in the body, which again is entirely a result of malfunctioning gi mic ... | 2012 | 24570901 |
| modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice. | structural disruption of gut microbiota and associated inflammation are considered important etiological factors in high fat diet (hfd)-induced metabolic syndrome (ms). three candidate probiotic strains, lactobacillus paracasei cncm i-4270 (lc), l. rhamnosus i-3690 (lr) and bifidobacterium animalis subsp. lactis i-2494 (ba), were individually administered to hfd-fed mice (10(8) cells day(-1)) for 12 weeks. each strain attenuated weight gain and macrophage infiltration into epididymal adipose tis ... | 2014 | 24936764 |
| molecular analysis and clinical significance of lactobacillus spp. recovered from clinical specimens presumptively associated with disease. | lactobacillus spp. are part of the normal human flora and are generally assumed to be nonpathogenic. we determined the genotypic identification of >100 lactobacillus isolates from clinical specimens in the context of presumed pathogenic potential (e.g., recovered as the single/predominant isolate from a sterile site or at ≥10(5) cfu/ml from urine). this study assessed the clinical significance and the frequency of occurrence of each lactobacillus sp. we identified 16 species of lactobacillus by ... | 2014 | 24131686 |
| fermentation process for production of apple-based kefir vinegar: microbiological, chemical and sensory analysis. | the aim of this study was to develop a kefir apple-based vinegar and evaluate this fermentation process using new methodology with biospeckle laser. brazilian kefir grains were inoculated in apple must for vinegar production. in this study, the microbial community present in kefir, and correspondent vinegar, was investigated using matrix assisted laser desorption/ionization - time of flight mass spectrometry (maldi-tof ms) technique. saccharomyces cerevisiae, lactobacillus paracasei, lactobacill ... | 2017 | 28283415 |
| microbial diversity and biochemical characteristics of borassus akeassii wine. | palm wine produced traditionally and consumed by many people in the south-west of burkina faso is subject to alteration. in this study, we carried out a follow-up of two palm wines' fermentation during the 10 days in which palm wines are classically produced and consumed. we monitored biochemical characteristics of fermenting wines as well as followed the microflora kinetics using culture-dependent and culture-independent methods. the analysis of the acid content and the bacterial population rev ... | 2016 | 27450560 |
| antimicrobial effect of gaseous and aqueous ozone on caries pathogen microorganisms grown in biofilms. | to evaluate the anti-microbial effect of gaseous and aqueous ozone on biofilms of caries pathogen microorganisms with regard to concentration and time dependency. | 2014 | 25208360 |
| in vitro evaluation of mtad and nisin in combination against common pathogens associated with root canal infection. | many pathogenic microorganisms were found in an infected root canal. the object of this study was to evaluate the effect of mtad in combination with nisin on the pathogens associated with root canal infection. | 2012 | 22414835 |
| comparison of riboflavin and toluidine blue o as photosensitizers for photoactivated disinfection on endodontic and periodontal pathogens in vitro. | photoactivated disinfection has a strong local antimicrobial effect. in the field of dentistry it is an emerging adjunct to mechanical debridement during endodontic and periodontal treatment. in the present study, we investigate the effect of photoactivated disinfection using riboflavin as a photosensitizer and blue led light for activation, and compare it to photoactivated disinfection with the widely used combination of toluidine blue o and red light. riboflavin is highly biocompatible and can ... | 2015 | 26469348 |
| antifungal activity of lactobacillus sp. bacteria in the presence of xylitol and galactosyl-xylitol. | lactic acid fermentation is a natural method of antimicrobial food protection. antagonistic activity of lactobacillus sp. bacteria, taking part in this process, is directed mainly against the same or other microorganisms. in this work we determine the impact of the presence of xylitol and galactosyl-xylitol on the antagonistic activity of 60 lactobacillus sp. strains against indicator molds (alternaria alternata, alternaria brassicicola, aspergillus niger, fusarium latenicum, geotrichum candidum ... | 2016 | 27294124 |
| lactate- and acetate-based cross-feeding interactions between selected strains of lactobacilli, bifidobacteria and colon bacteria in the presence of inulin-type fructans. | cross-feeding interactions were studied between selected strains of lactobacilli and/or bifidobacteria and butyrate-producing colon bacteria that consume lactate but are not able to degrade inulin-type fructans (itf) in a medium for colon bacteria (supplemented with itf as energy source and acetate when necessary). degradation of oligofructose by lactobacillus acidophilus ibb 801 and inulin by lactobacillus paracasei 8700:2 and bifidobacterium longum lmg 11047 resulted in the release of free fru ... | 2017 | 27810444 |
| effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation. | in this study, the effect that 5 fermented broths of lactic acid bacteria (lab) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. the fermented broth from lactococcus lactis c660 had a growth inhibitory effect on escherichia coli k92 that reached of 31%, 19% to pseudomonas fluorescens, and 76% to staphylococcus epidermidis. the growth of staph. epidermidis was negatively affected to 90% by lc. lactis 11454 broth, whereas the growth ... | 2016 | 26851857 |
| identification of microbiota present on the surface of taleggio cheese using pcr-dgge and rapd-pcr. | microbial dna from 9 batches of taleggio pdo cheese sampled at various times during ripening, brines, swabs of wooden shelves used for cheese dry-salting, and 13 commercial cheeses were analyzed by denaturing gradient gel electrophoresis (pcr-dgge) and/or random amplification of polymorphic dna (rapd-pcr). sequencing allowed the detection of 12 genera, 27 species, and 2 unclassified bacteria. molecular analysis allowed for the detection of microorganisms not previously associated with taleggio s ... | 2012 | 23094819 |
| antibacterial activity of a cell wall hydrolase from lactobacillus paracasei nrrl b-50314 produced by recombinant bacillus megaterium. | the cell-free supernatant (cfs) from lactobacillus paracasei nrrl b-50314 culture has been previously reported as containing antibacterial activity against a wide variety of gram-positive bacteria. the cfs protein gel slice corresponding to antibacterial activities was subjected to trypsin digestion and ion trap mass (gel/lc-ms/ms) analysis. blastp search of the resulted iqavisiaeqqigkp sequence led to a hypothetical cell-wall associated hydrolase (designated as cwh here) from lactobacillus para ... | 2015 | 25533632 |
| effects of biosurfactants on the viability and proliferation of human breast cancer cells. | biosurfactants are molecules with surface activity produced by microorganisms that can be used in many biomedical applications. the anti-tumour potential of these molecules is being studied, although results are still scarce and few data are available regarding the mechanisms underlying such activity. in this work, the anti-tumour activity of a surfactin produced by bacillus subtilis 573 and a glycoprotein (bioeg) produced by lactobacillus paracasei subsp. paracasei a20 was evaluated. both biosu ... | 2014 | 24949273 |
| evaluation of bean and soy tempeh influence on intestinal bacteria and estimation of antibacterial properties of bean tempeh. | in this study the effect of bean tempeh on the growth of bacillus subtilis, escherichia coli, lactobacillus acidophilus and lactobacillus paracasei bacteria was investigated. antibacterial activity was observed only in relation to the bacteria bacillus subtilis. the effect of tempeh products on human intestinal microflora was also assessed. bean and soy tempeh were culinarily processed and next digested in conditions simulating the human digestive tract (one of the digestive tracts was equipped ... | 2013 | 24053022 |
| bacillus subtilis natto: a non-toxic source of poly-γ-glutamic acid that could be used as a cryoprotectant for probiotic bacteria. | it is common practice to freeze dry probiotic bacteria to improve their shelf life. however, the freeze drying process itself can be detrimental to their viability. the viability of probiotics could be maintained if they are administered within a microbially produced biodegradable polymer - poly-γ-glutamic acid (γ-pga) - matrix. although the antifreeze activity of γ-pga is well known, it has not been used for maintaining the viability of probiotic bacteria during freeze drying. the aim of this s ... | 2013 | 23829836 |
| proteolysis in goat "coalho" cheese supplemented with probiotic lactic acid bacteria. | this study aimed to analyse the proteolytic effects of adding isolated and combined probiotic strains to goat "coalho" cheese. the cheeses were: qs - with culture start, composed by lactococcus lactis subsp. lactis and l. lactis subsp. cremoris (r704); qla - with lactobacillus acidophilus (la-5); qlp - with lactobacillus paracasei subsp. paracasei (l. casei 01); qb - with bifidobacterium animalis subsp. lactis (bb 12); and qc, co-culture with the three probiotic microorganisms. the cheeses were ... | 2016 | 26593502 |
| prebiotic preferences of human lactobacilli strains in co-culture with bifidobacteria and antimicrobial activity against clostridium difficile. | to evaluate robustness, prebiotic utilization of lactobacillus paracasei f8 and lactobacillus plantarum f44 in mono- and co-cultures with bifidobacterium breve 46 and bifidobacterium animalis sub sp. lactis 8 : 8 and antimicrobial activity of co-culture against clostridium difficile. | 2015 | 26381324 |
| human intestinal mucosa-associated lactobacillus and bifidobacterium strains with probiotic properties modulate il-10, il-6 and il-12 gene expression in thp-1 cells. | lactobacilli and bifidobacteria are considered one of the permanent genera of the physiological human intestinal microbiota and represent an enormous pool of potential probiotic candidates. approximately 450 isolates of presumptive lactobacillus or bifidobacterium strains were obtained from bioptic samples of colonic and ileal mucosa from 15 adolescents aged 12 to 18 years. on the basis of randomly amplified polymorphic dna (rapd)-pcr analysis, 20 strains were selected for further taxonomic clas ... | 2015 | 25391349 |
| probiotics in the prevention of eczema: a randomised controlled trial. | to evaluate a multistrain, high-dose probiotic in the prevention of eczema. | 2014 | 24947281 |
| probiotic bacteria survive in cheddar cheese and modify populations of other lactic acid bacteria. | starter lactic acid bacteria in cheddar cheese face physico-chemical stresses during manufacture and ageing that alter their abilities to survive and to interact with other bacterial populations. nonstarter bacteria are derived from milk handling, cheese equipment and human contact during manufacture. probiotic bacteria are added to foods for human health benefits that also encounter physiological stresses and microbial competition that may mitigate their survival during ageing. we added probiot ... | 2014 | 24905221 |
| gut balance, a synbiotic supplement, increases fecal lactobacillus paracasei but has little effect on immunity in healthy physically active individuals. | synbiotic supplements, which contain multiple functional ingredients, may enhance the immune system more than the use of individual ingredients alone. a double blind active controlled parallel trial over a 21 d exercise training period was conducted to evaluate the effect of gut balance™, which contains lactobacillus paracasei subsp. paracasei (l. casei 431®), bifidobacterium animalis ssp. lactis (bb-12®), lactobacillus acidophilus (la-5®), lactobacillus rhamnosus (lgg®), two prebiotics (raftili ... | 2017 | 22572834 |
| lactobacillus rhamnosus gg increases toll-like receptor 3 gene expression in murine small intestine ex vivo and in vivo. | administration of lactobacillus rhamnosus gg (lgg) has been reported to be therapeutically effective against acute secretory diarrhoea resulting from the structural and functional intestinal mucosal lesions induced by rotavirus infection; however, the underlying mechanisms remain to be completely elucidated. because toll-like receptor 3 (tlr3) plays a key role in the innate immune responses following the recognition of rotavirus, the present study examined whether lgg influences tlr3 gene expres ... | 2016 | 27013459 |
| role of probiotics in the prevention and treatment of meticillin-resistant staphylococcus aureus infections. | meticillin-resistant staphylococcus aureus (mrsa) is a multidrug-resistant micro-organism and is the principal nosocomial pathogen worldwide. following initial in vitro experiments demonstrating that lactobacillus acidophilus cl1285(®) and lactobacillus casei lbc80r(®) commercial strains exhibit antibacterial activity against clinical mrsa isolates, we conducted a literature search to find any evidence of probiotic efficacy in decolonisation or treatment of s. aureus infection. as summarised bel ... | 2013 | 24071026 |
| pyrosequencing analysis reveals changes in intestinal microbiota of healthy adults who received a daily dose of immunomodulatory probiotic strains. | the colon microbiota plays a crucial role in human gastrointestinal health. current attempts to manipulate the colon microbiota composition are aimed at finding remedies for various diseases. we have recently described the immunomodulatory effects of three probiotic strains (lactobacillus rhamnosus cncm i-4036, lactobacillus paracasei cncm i-4034, and bifidobacterium breve cncm i-4035). the goal of the present study was to analyze the compositions of the fecal microbiota of healthy adults who re ... | 2015 | 26016655 |
| effects of lactobacillus paracasei cncm i-4034, bifidobacterium breve cncm i-4035 and lactobacillus rhamnosus cncm i-4036 on hepatic steatosis in zucker rats. | we have previously described the safety and immunomodulatory effects of lactobacillus paracasei cncm i-4034, bifidobacterium breve cncm i-4035 and lactobacillus rhamnosus cncm i-4036 in healthy volunteers. the scope of this work was to evaluate the effects of these probiotic strains on the hepatic steatosis of obese rats. we used the zucker rat as a genetic model of obesity. zucker-lepr(fa/fa) rats received one of three probiotic strains, a mixture of l. paracasei cncm i-4034 and b. breve cncm i ... | 2014 | 24852284 |
| safety and immunomodulatory effects of three probiotic strains isolated from the feces of breast-fed infants in healthy adults: setoprob study. | we previously described the isolation and characterization of three probiotic strains from the feces of exclusively breast-fed newborn infants: lactobacillus paracasei cncm i-4034, bifidobacterium breve cncm i-4035 and lactobacillus rhamnosus cncm i-4036. these strains were shown to adhere to intestinal mucus in vitro, to be sensitive to antibiotics and to resist biliary salts and low ph. in the present study, a multicenter, randomized, double-blind, placebo-controlled trial with 100 healthy vol ... | 2013 | 24205115 |
| competitive inhibition of three novel bacteria isolated from faeces of breast milk-fed infants against selected enteropathogens. | numerous in vitro and in vivo studies conducted using different probiotic micro-organisms have demonstrated their ability to interfere with the growth and virulence of a variety of enteropathogens. the reported beneficial effects of the use of probiotics to complement antibiotic therapy or prevent diarrhoea or gastrointestinal infection in infants have increased in recent years. in the present study, we demonstrated the capacity of supernatants obtained from three novel probiotics (lactobacillus ... | 2013 | 23360882 |
| isolation, identification and characterisation of three novel probiotic strains (lactobacillus paracasei cncm i-4034, bifidobacterium breve cncm i-4035 and lactobacillus rhamnosus cncm i-4036) from the faeces of exclusively breast-fed infants. | the aim of the present study was to isolate, identify and characterise novel strains of lactic acid bacteria and bifidobacteria with probiotic properties from the faeces of exclusively breast-fed infants. of the 4680 isolated colonies, 758 exhibited resistance to low ph and tolerance to high concentrations of bile salts; of these, only forty-two exhibited a strong ability to adhere to enterocytes in vitro. the identities of the isolates were confirmed by 16s ribosomal rna (rrna) sequencing, whic ... | 2013 | 23360881 |
| long-term safety and efficacy of perinatal probiotic intervention: evidence from a follow-up study of four randomized, double-blind, placebo-controlled trials. | societies worldwide are faced with a progressive increase in immune-mediated health problems such as allergic, autoimmune, and inflammatory diseases, as well as obesity. perinatal administration of specific probiotic bacteria is an attractive approach in reducing the risk of these conditions, but long-term efficacy and safety data are lacking. the aim here was to evaluate the clinical benefit and long-term safety of specific probiotics administered during the perinatal period. | 2017 | 27779809 |
| in vitro inhibition of klebsiella pneumoniae by lactobacillus delbrueckii subsp. delbrueckii ldd01 (dsm 22106): an innovative strategy to possibly counteract such infections in humans? | to determine the in vitro antimicrobial activity of selected lactobacillus strains isolated from the feces of healthy humans against klebsiella pneumoniae. | 2016 | 27741158 |
| optimization of fermentation parameters to study the behavior of selected lactic cultures on soy solid state fermentation. | the use of solid fermentation substrate (ssf) has been appreciated by the demand for natural and healthy products. lactic acid bacteria and bifidobacteria play a leading role in the production of novel functional foods and their behavior is practically unknown in these systems. soy is an excellent substrate for the production of functional foods for their low cost and nutritional value. the aim of this work was to optimize different parameters involved in solid state fermentation (ssf) using sel ... | 2015 | 25498472 |
| effects of bile salt deconjugation by probiotic strains on the survival of antibiotic-resistant foodborne pathogens under simulated gastric conditions. | this study was designed to evaluate the effects of bile acid deconjugation by probiotic strains on the antibiotic susceptibility of antibiotic-sensitive and multiple antibiotic-resistant salmonella typhimurium and staphylococcus aureus. eight probiotic strains, bifidobacterium longum b6, lactobacillus acidophilus adh, lactobacillus brevis kacc 10553, lactobacillus casei kacc 12413, lactobacillus paracasei atcc 25598, lactobacillus rhamnosus gg, leuconostoc mesenteroides kacc 12312, and pediococc ... | 2012 | 22691477 |
| effect of human isolated probiotic bacteria on preventing campylobacter jejuni colonization of poultry. | this study was performed in order to determine whether human isolated probiotic bacteria can be effective in reducing campylobacter jejuni infection of chicken intestinal cells, in vitro, and in decreasing its colonization abilities within the chicken gut. our results show that the probiotic strains lactobacillus paracasei j. r, l. rhamnosus 15b, l. lactis y, and l. lactis foa had a significant effect on c. jejuni invasion of chicken primary cells, with the strongest inhibitory effect detected w ... | 2015 | 25585278 |
| lactobacillus paracasei modulates the immune system of galleria mellonella and protects against candida albicans infection. | probiotics have been described as a potential strategy to control opportunistic infections due to their ability to stimulate the immune system. using the non-vertebrate model host galleria mellonella, we evaluated whether clinical isolates of lactobacillus spp. are able to provide protection against candida albicans infection. among different strains of lactobacillus paracasei, lactobacillus rhamnosus and lactobacillus fermentum, we verified that l. paracasei 28.4 strain had the greatest ability ... | 2017 | 28267809 |
| antimicrobial efficacy of probiotic-containing toothpastes: an in vitro evaluation. | aim to evaluate, in vitro antimicrobial ability of two probiotic toothpastes (one containing lactobacillus paracasei, other containing lactobacillus acidophilus) and one toothpaste without probiotic separately, and in a combination with two different mouthrinses (one containing essential oils and the other containing hexitidine). methods antimicrobial susceptibility was checked by using the ditch method and clinical laboratory standard institute (clsi) guidelines. two different toothpastes with ... | 2017 | 28165440 |
| in vitro and in vivo examination of anticolonization of pathogens by lactobacillus paracasei fj861111.1. | very limited information exists on the exclusion of pathogens by probiotics in the gut of the host challenged with pathogens. in this study, we tested probiotic characteristics in vitro and anticolonization ability of lactobacillus paracasei fj861111.1 in mice infected with selected pathogenic microorganisms. the in vitro results indicated that l. paracasei fj861111.1 had a high survival in acidic conditions at ph 2.5 and bile salt concentration at 0.3%, and strong inhibition ability against com ... | 2015 | 26254535 |
| isolation and characterisation of lactic acid bacteria from donkey milk. | during the last years the interest in donkey milk has increased significantly mainly because of its compelling functional elements. even if the composition and nutritional properties of donkey milk are known, its microbiota is less studied. this research communication aimed to provide a comprehensive characterisation of the lactic acid bacteria in raw donkey milk. rapd-pcr assay combined with 16s rdna sequencing analysis were used to describe the microbial diversity of several donkey farms in th ... | 2016 | 27600975 |
| neutralization of clostridium difficile toxin b mediated by engineered lactobacilli that produce single-domain antibodies. | clostridium difficile is the primary cause of nosocomial antibiotic-associated diarrhea in the western world. the major virulence factors of c. difficile are two exotoxins, toxin a (tcda) and toxin b (tcdb), which cause extensive colonic inflammation and epithelial damage manifested by episodes of diarrhea. in this study, we explored the basis for an oral antitoxin strategy based on engineered lactobacillus strains expressing tcdb-neutralizing antibody fragments in the gastrointestinal tract. va ... | 2015 | 26573738 |
| nutrition economic evaluation of a probiotic in the prevention of antibiotic-associated diarrhea. | antibiotic-associated diarrhea (aad) is common and frequently more severe in hospitalized elderly adults. it can lead to increased use of healthcare resources. we estimated the cost-effectiveness of a fermented milk (fm) with probiotic in preventing aad and in particular clostridium difficile-associated diarrhea (cdad). | 2014 | 24596556 |
| probiotics reduce symptoms of antibiotic use in a hospital setting: a randomized dose response study. | probiotics are known to reduce antibiotic associated diarrhea (aad) and clostridium difficile associated diarrhea (cdad) risk in a strain-specific manner. the aim of this study was to determine the dose-response effect of a four strain probiotic combination (howaru(®) restore) on the incidence of aad and cdad and severity of gastrointestinal symptoms in adult in-patients requiring antibiotic therapy. patients (n=503) were randomized among three study groups: howaru(®) restore probiotic 1.70×10(1 ... | 2014 | 24291194 |
| enhanced treatment of tannery wastewater in an integrated multistage bioreactor (imbr) by the predominant bacterial strains enriched from marine sediments. | an innovative integrated multistage bioreactor (imbr) system, which was augmented with three predominant bacterial strains (lactobacillus paracasei cl1107, pichia jadinii cl1705, and serratia marcescens cl1502) isolated from marine sediments, was developed to treat real tannery wastewater without performing physicochemical pretreatment, with the potential to reduce the generation of waste sludge and odors. the performance of the imbr treatment system, with and without the inclusion of the predom ... | 2016 | 26901723 |
| microbiological characterization using combined culture dependent and independent approaches of casizolu pasta filata cheese. | casizolu is a traditional sardinian (italy) pasta filata cheese made with cow raw milk belonging to sardo-modicana and/or bruno-sarda breeds added with natural whey starter. this work aims to describe the traditional technology of this product and to evaluate the microbial groups/species involved in the first month of ripening. | 2016 | 26551888 |
| identification of lactic acid bacteria in traditional fermented yak milk and evaluation of their application in fermented milk products. | in this study, 53 strains of lactic acid bacteria (lab) isolated from xueo, a traditional fermented yak milk in the western sichuan plateau of china, were identified and their use in fermented milk was evaluated. all gram-positive and catalase-negative strains were divided into 6 groups at the level of 87% similarity using amplified ribosomal dna restriction analysis. these groups were identified as 6 species using 16s rdna sequence analysis and atpa gene analysis. the dominant lab strains in xu ... | 2012 | 22365191 |
| screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil. | lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ... | 2016 | 26608755 |
| an in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (mta) against five oral bacteria. | the purpose of this study was to evaluate the antibacterial ability of three mta (mta-angelus, endocem mta, and proroot mta) against five typical oral bacteria (streptococcus mutans, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus paracasei, and porphyromonas gingivalis). | 2015 | 26263538 |
| probiotic features of lactobacillus strains isolated from ragusano and pecorino siciliano cheeses. | in the present study 177 lactobacillus spp. strains, isolated from ragusano and pecorino siciliano cheeses, were in vitro screened for probiotic traits, and their characteristics were compared to those of lactobacillus rhamnosus gg, commercial strain. based on acidic and bile salt resistance, thirteen lactobacillus strains were selected. the multiplex-pcr application revealed that nine strains belonged to l. rhamnosus species and four to lactobacillus paracasei species. all selected strains were ... | 2015 | 25998823 |
| biodiversity and γ-aminobutyric acid production by lactic acid bacteria isolated from traditional alpine raw cow's milk cheeses. | "nostrano-cheeses" are traditional alpine cheeses made from raw cow's milk in trentino-alto adige, italy. this study identified lactic acid bacteria (lab) developing during maturation of "nostrano-cheeses" and evaluated their potential to produce γ-aminobutyric acid (gaba), an immunologically active compound and neurotransmitter. cheese samples were collected on six cheese-making days, in three dairy factories located in different areas of trentino and at different stages of cheese ripening (24 ... | 2015 | 25802859 |
| a zn-dependent metallopeptidase is responsible for sensitivity to lsbb, a class ii leaderless bacteriocin of lactococcus lactis subsp. lactis bgmn1-5. | lactococcus lactis subsp. lactis bgmn1-5 produces a leaderless class ii bacteriocin called lsbb. to identify the receptor for lsbb, a cosmid library of the lsbb-sensitive strain bgmn1-596 was constructed. about 150 cosmid clones were individually isolated and transferred to lsbb-resistant mutants of bgmn1-596. cosmid pazilcos/mn2, carrying a 40-kb insert, was found to restore lsbb sensitivity in lsbb-resistant mutants. further subcloning revealed that a 1.9-kb fragment, containing only one open ... | 2013 | 24123824 |
| technological and safety properties of lactic acid bacteria isolated from spanish dry-cured sausages. | technological and safety-related properties were analyzed in lactic acid bacteria isolated from spanish dry-cured sausages in order to select them as starter cultures. in relation to technological properties, all the strains showed significative nitrate reductase activity; lactobacillus plantarum, lactobacillus paracasei and 52% of the enterococcus faecium strains showed lipolytic activity and only lactobacillus sakei strains (43%) were able to form biofilms. related to safety aspects, e. faeciu ... | 2013 | 23743032 |
| bioconversion of oleuropein to hydroxytyrosol by lactic acid bacteria. | the aim of this work is to study the conversion of oleuropein-a polyphenol present in olives and olive oil by-products-into hydroxytyrosol, a polyphenol with antioxidant and antibacterial properties. the hydrolysis reaction is performed by lactic acid bacteria. six bacterial strains (lactobacillus plantarum 6907, lactobacillus paracasei 9192, lactobacillus casei, bifidobacterium lactis bo, enterococcus faecium 32, lactobacillus lafti 10) were tested under aerobic and anaerobic conditions. the ol ... | 2012 | 22806118 |
| selection of indigenous lactobacillus paracasei cd4 and lactobacillus gastricus btm 7 as probiotic: assessment of traits combined with principal component analysis. | the population of himalayan region is known to consume variety of fermented and non fermented foods and as a result they have been benefited in terms of overall health, because of the associated beneficial microbes. therefore, the focus of the present study was to identify new strains of lactic acid bacteria (lab) from dairy products such as milk (cow, goat, buffalo) and fermented products (curd and buttermilk) with properties suitable for use as probiotic cultures. | 2017 | 28236643 |
| microbiological quality and variability of natural microbiota in croatian cheese maturing in lambskin sacks. | as in the traditional production of cheese in lambskin sacks raw cow's or sheep's milk is mostly used, the purpose of this study is to see how the production affects the microbiological quality of the cheese. to do that, we tested 39 samples of raw cow's and sheep's milk, curd, ripened cheese (15, 30 and 45 days) and lambskin sacks for native microbial population. two-thirds of the milk, curd and cheese samples had higher counts of staphylococci and enterobacteria than permitted by regulations. ... | 2016 | 27904402 |
| functional analysis of plantaricin e and its mutant by heterologous expression in escherichia coli. | plantaricins are a group of ribosomally synthesized antimicrobial peptides in lactobacillus plantarum that exert antimicrobial activities against some foodborne pathogens. in this study, we observed that plantaricin e in l. plantarum 163 was missing 19 amino acids (plne mutant amino acid sequence: fnrggynfgksvrh, plne amino acid sequence: fnrggynfgksvrhvvdaigsvagirgilksir). in order to study the effects of mutant plne, plne mutant genes with and without the signal peptide were cloned from the l. ... | 2016 | 27854041 |
| antibacterial metabolites secreted under glucose-limited environment of the mimicked proximal colon model by lactobacilli abundant in infant feces. | the most abundance of anti-salmonella lactic acid bacteria (lab) was found in feces of naturally born, exclusively breastfed thai infants. six strains of lactobacillus plantarum and one strain of lactobacillus paracasei were selected and identified. in the co-cultivation assay, l. plantarum subsp. plantarum i62 showed the strongest and broadest antibacterial activity against escherichia coli, shigella sonnei, salmonella paratyphi a, and salmonella typhimurium sa 2093 under the mimicked proximal ... | 2016 | 27188778 |
| eps-sj exopolisaccharide produced by the strain lactobacillus paracasei subsp. paracasei bgsj2-8 is involved in adhesion to epithelial intestinal cells and decrease on e. coli association to caco-2 cells. | the aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate lactobacillus paracasei subsp. paracasei bgsj2-8, in the adhesion to intestinal epithelial cells and a decrease in escherichia coli's association with caco-2 cells. annotation of the bgsj2-8 genome showed the presence of a gene cluster, epssj, which encodes the biosynthesis of the strain-specific exopolysaccharide eps-sj, detected as two fractions (p1 and p2) by size exclusion chromatography ... | 2016 | 27014210 |
| changes on fecal microbiota in rats exposed to permethrin during postnatal development. | alteration of the gut microbiota through diet and environmental contaminants may disturb the mammalian digestive system, leading to various diseases. because most exposure to environmentally pyrethroid pesticides such as permethrin (perm) occurs through the diet, the commensal gut microbiota is likely to be exposed to perm. the study aimed at evaluating the effect of low-dose exposure to perm in early life on the composition of fecal microbiota in rats. over a 4-month follow-up period, fecal mic ... | 2016 | 26898931 |
| the effects of inulin on characteristics of lactobacillus paracasei td3 (ibrc-m 10784) as probiotic bacteria in vitro. | probiotics, as live microbial food ingredients or functional foods, are often related to health promotion and diseases prevention. the growth of these beneficial probiotics was improved by non-digestible food ingredients that are defined as prebiotics. inulin is one of the known prebiotics that improves the gastrointestinal health. | 2016 | 26838078 |
| assessment of characteristics and functional properties of lactobacillus species isolated from kimchi for dairy use. | the objective of this study was to identify lactic acid bacteria (lab) isolated from kimchi and to evaluate its characteristics and functional properties for application in fermented dairy products as a probiotic or commercial starter culture. eight stains isolated from kimchi were selected through an investigation of phenotypic characteristics. two strains (dk211 and dk303) were identified as lactobacillus plantarum, another two (dk207 and dk215) as lactobacillus paracasei, and one (dk301) as l ... | 2015 | 26761848 |
| expression of a novel bacteriocin-the plantaricin pln1-in escherichia coli and its functional analysis. | a potential bacteriocin gene was isolated from 18575 orfs by bioinformatics methods. it was named pln1, and cloned into pet32a. then, it was expressed as a thioredoxin-pln1 fusion protein in escherichia coli bl21 (de3). the fusion protein was purified by ni-nta, and thioredoxin was removed by enterokinase. finally, pln1 was purified using a cation affinity column. the yields of fused and cleaved pln1 peptides were 100-110 mg/l and 9-11 mg/l, respectively. pln1 was stable in an acidic environment ... | 2016 | 26586613 |
| [comparison of expression and antibacterial activities of recombinant porcine lactoferrin expressed in four lactobacillus species]. | the coding sequence for the mature peptide of porcine lactoferrin (plf) was synthesized according to the codon usage of lactobacillus, to establish optimized porcine lactoferrin lactobacillus expression system. the gene was ligated into the xho i/bamh i site of lactobacillus expression vector ppg612.1 and the recombinant plasmid ppg612.1-plf was transformed individually into lactobacillus casei atcc393, lactobacillus pentosus klds1.0413, lactobacillus plantarum klds1.0344 or lactobacillus paraca ... | 2014 | 25720152 |
| induction of pro-inflammatory gene expression by escherichia coli and mycotoxin zearalenone contamination and protection by a lactobacillus mixture in porcine ipec-1 cells. | this work investigated the effect of escherichia coli k88 and zearalenone contamination on pro-inflammatory gene expression (toll like receptors, cytokines) and signalling molecules and the protective activity of a mixture of lactobacilli sp. (lactobacillus plantarum, lactobacillus acidofilus and lactobacillus paracasei) in porcine intestinal epithelial cells as part of the local immune system. ipec-1 cell monolayer was exposed for 1 h to the individual or combined action of e. coli, zearalenone ... | 2015 | 25640651 |
| development of lactobacillus paracasei harboring nucleic acid-hydrolyzing 3d8 scfv as a preventive probiotic against murine norovirus infection. | the protein 3d8 single-chain variable fragment (3d8 scfv) has potential anti-viral activity due to its ability to penetrate into cells and hydrolyze nucleic acids. probiotic lactobacillus paracasei engineered to secrete 3d8 scfv for oral administration was used to test the anti-viral effects of 3d8 scfv against gastrointestinal virus infections. we found that injection of 3d8 scfv into the intestinal lumen resulted in the penetration of 3d8 scfv into the intestinal villi and lamina propria. 3d8 ... | 2015 | 25487889 |
| effectiveness of an association of a cranberry dry extract, d-mannose, and the two microorganisms lactobacillus plantarum lp01 and lactobacillus paracasei lpc09 in women affected by cystitis: a pilot study. | urinary tract infections (utis) are the most common bacterial infection in women. most utis are acute uncomplicated cystitis caused by escherichia coli (86%). this study was undertaken to assess the effectiveness of an association of a cranberry dry extract, d-mannose, a gelling complex composed of the exopolysaccharides produced by streptococcus thermophilus st10 (dsm 25246) and tara gum, as well as the 2 microorganisms lactobacillus plantarum lp01 (lmg p-21021) and lactobacillus paracasei lpc0 ... | 2015 | 25291140 |
| characterization of pmc11, a plasmid with dual origins of replication isolated from lactobacillus casei mcj and construction of shuttle vectors with each replicon. | many lactic acid bacteria carry different plasmids, particularly those that replicate via a theta mechanism. here we describe lactobacillus casei mcj(cctcc ab20130356), a new isolate that contains pmc11, carrying two distinct theta-type replicons. each replicon contained an iteron in the origin of replication (oriv1 or oriv2) and a gene coding for the replicase (repa_1 or repb_1), both of which are essential for plasmid replication. escherichia coli/lactobacillus shuttle vectors were constructed ... | 2014 | 24652065 |
| lactobacillus paracasei subsp. paracasei lc01 positively modulates intestinal microflora in healthy young adults. | lactobacillus paracasei subsp. paracasei lc01 (lc01) can tolerate intestinal stresses and has antioxidant activity. to evaluate the effect of the bacterium on human intestinal microflora, a randomized, double-blind, placebo-controlled human trial was carried out. fifty-two healthy adult volunteers were randomized equally to two groups. one group consumed 12% (wt/vol) skimmed milk supplemented with 10(10) cfu of lc01 each day for the 4-week treatment period, and then consumed placebo in the next ... | 2013 | 24385355 |
| effects of a lactobacillus paracasei b21060 based synbiotic on steatosis, insulin signaling and toll-like receptor expression in rats fed a high-fat diet. | insulin resistance (ir) has been identified as crucial pathophysiological factor in the development and progression of non-alcoholic fatty liver disease (nafld). although mounting evidence suggests that perturbation of gut microflora exacerbates the severity of chronic liver diseases, therapeutic approaches using synbiotic has remained overlooked. here, we show that a synbiotic composed by lactobacillus paracasei b21060 plus arabinogalactan and fructo-oligosaccharides lessens nafld progression i ... | 2014 | 24314869 |
| tailoring the probiotic potential of non-starter lactobacillus strains from ripened parmigiano reggiano cheese by in vitro screening and principal component analysis. | non-starter lactic acid bacteria (nslab) inhabiting fermented food have been recently revised as source of probiotic strains. here, we in vitro assessed the potential probiotic aptitude of a de-replicated set of nslab previously isolated from long ripened parmigiano reggiano cheeses (22 lactobacillus rhamnosus, 18 lactobacillus paracasei, 3 lactobacillus casei, 2 lactobacillus harbinensis, and 2 lactobacillus fermentum). most strains showed moderate to good resistance to biological barriers, inc ... | 2014 | 24290648 |
| bioprotection of ready-to-eat probiotic artichokes processed with lactobacillus paracasei lmgp22043 against foodborne pathogens. | the survival of 3 pathogens listeria monocytogenes atcc19115, salmonella enterica subsp. enterica atcc13311, and escherichia coli atcc8739 was evaluated over time in ready-to-eat (rte) artichoke products processed or not with the probiotic strain lactobacillus paracasei lmgp22043. both probiotic and standard products (final ph about 4.0; aw = 0.98) dressed with oil and packaged in modified atmosphere were inoculated with pathogens at a level of about 3 log cfu/g and stored at 4 ºc for 45 d. path ... | 2013 | 24245894 |
| enteroglial-derived s100b protein integrates bacteria-induced toll-like receptor signalling in human enteric glial cells. | enteric glial cells (egc) have been suggested to participate in host-bacteria cross-talk, playing a protective role within the gut. the way egc interact with microorganisms is still poorly understood. we aimed to evaluate whether: egc participate in host-bacteria interaction; s100b and toll-like receptor (tlr) signalling converge in a common pathway leading to nitric oxide (no) production. | 2014 | 23292665 |
| inhibition of fe-induced colon oxidative stress by lactobacilli in mice. | iron (fe) can promote hydrogen peroxide (h(2)o(2)) and hydroxyl radical generation in the colonic surface and promote growth of fe-dependent bacteria. some lactobacillus strains are resistant to oxygen free-radicals, allowing them to survive in a fe-modulated mucosal environment and influence colon microbial ecology and redox state. here, we investigated the capacity of lactobacilli with different antioxidant abilities to modify the bacterial profile and prevent oxidative stress in the colon of ... | 2013 | 22990462 |
| antimicrobial susceptibility of microflora from ovine cheese. | strains identified in ovine cheese and bryndza by matrix-assisted laser desorption/ionization time-of-flight analysis belonged to ten species of non-enterococcal lactic acid bacteria and included lactobacillus casei/lactobacillus paracasei, lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus helveticus, lactobacillus delbrueckii, lactobacillus fermentum, lactobacillus brevis, lactococcus lactis, pediococcus pentosaceus and pediococcus acidilactici. the susceptibility toward antibioti ... | 2012 | 22491987 |
| colonizing the embryonic zebrafish gut with anaerobic bacteria derived from the human gastrointestinal tract. | the zebrafish has become increasingly popular for microbiological research. it has been used as an infection model for a variety of pathogens, and is also emerging as a tool for studying interactions between a host and its resident microbial communities. the mouse microbiota has been transplanted into the zebrafish gut, but to our knowledge, there has been no attempt to introduce a bacterial community derived from the human gut. we explored two methods for colonizing the developing gut of 5-day- ... | 2013 | 23530761 |
| investigation of the instability and low water kefir grain growth during an industrial water kefir fermentation process. | a poorly performing industrial water kefir production process consisting of a first fermentation process, a rest period at low temperature, and a second fermentation process was characterized to elucidate the causes of its low water kefir grain growth and instability. the frozen-stored water kefir grain inoculum was thawed and reactivated during three consecutive prefermentations before the water kefir production process was started. freezing and thawing damaged the water kefir grains irreversib ... | 2017 | 28070662 |
| a methodological approach to screen diverse cheese-related bacteria for their ability to produce aroma compounds. | microorganisms play an important role in the development of cheese flavor. the aim of this study was to develop an approach to facilitate screening of various cheese-related bacteria for their ability to produce aroma compounds. we combined i) curd-based slurry medium incubated under conditions mimicking cheese manufacturing and ripening, ii) powerful method of extraction of volatiles, headspace trap, coupled to gas chromatography-mass spectrometry (hs-trap-gc-ms), and iii) metabolomics-based me ... | 2015 | 25475278 |
| visualization of consensus genome structure without using a reference genome. | standard graphical tools for whole genome comparison require a reference genome. however, any reference is also subject to annotation biases and rearrangements, and may not serve as the standard except for those of extensively studied model species. to fully exploit the rapidly accumulating sequence data from the recent sequencing technologies, genome comparison without any reference has been anticipated. | 2017 | 28361716 |
| lactoferrin adsorbed onto biomimetic hydroxyapatite nanocrystals controlling - in vivo - the helicobacter pylori infection. | the resistance of helicobacter pylori to the antibiotic therapy poses the problem to discover new therapeutic approaches. recently it has been stated that antibacterial, immunomodulatory, and antioxidant properties of lactoferrin are increased when this protein is surface-linked to biomimetic hydroxyapatite nanocrystals. | 2016 | 27384186 |
| improved eradication rate of standard triple therapy by adding bismuth and probiotic supplement for helicobacter pylori treatment in thailand. | helicobacter pylori (h. pylori) remains an important cause of gastric cancer and peptic ulcer disease worldwide. treatment of h. pylori infection is one of the effective ways to prevent gastric cancer. however, standard triple therapy for h. pylori eradication is no longer effective in many countries, including thailand. this study was designed to evaluate the efficacy of adding bismuth and probiotic to standard triple therapy for h. pylori eradication. | 2014 | 25520127 |
| lead biosorption of probiotic bacteria: effects of the intestinal content from laying hens. | this study investigated the effects and the possible mechanisms of intestinal content (ic) from laying hens on in vitro lead (pb(2+)) biosorption of four probiotic bacterial strains (bifidobacterium longum bb79, lactobacillus paracasei kgl6, lactobacillus pentosus ita23, and lactobacillus acidipiscis ita44). the total pb(2+) removal capacity of the four probiotic strains, with and without capsule polysaccharides (cpss), increased in the presence of ic compared to the control (without ic). sem im ... | 2017 | 28390025 |
| potential prebiotic properties of cashew apple (anacardium occidentale l.) agro-industrial byproduct on lactobacillus species. | the prebiotic effects of a cashew apple (anacardium occidentale l.) agro-industrial byproduct powder (cap) on different potentially probiotic lactobacillus strains, namely lactobacillus acidophilus la-05, lactobacillus casei l-26 and lactobacillus paracasei l-10, were assessed using in vitro experimental models. accordingly, the growth of the lactobacillus strains when cultivated in a broth containing cap (20 or 30 g l(-1) ), glucose (20 g l(-1) ) or fructooligosaccharides (fos) (20 g l(-1) ) wa ... | 2017 | 28111773 |
| survival of probiotics in hypromellose capsules with rice or potato maltodextrin excipient. | there is currently no authorized or established therapeutic level/dose of probiotics for proposed health benefits; however, a daily probiotic consumption of 10(8) to 10(10) cfu has been recommended. this study determined the survival of 5 individual probiotic strains, lactobacillus rhamnosus, lactobacillus paracasei, lactobacillus plantarum, lactobacillus acidophilus, and bifidobacterium lactis, along with a mixture of the 5 strains in hypromellose capsules with rice or potato maltodextrin at 4, ... | 2016 | 27681165 |
| characterization of biosurfactants produced by lactobacillus spp. and their activity against oral streptococci biofilm. | lactic acid bacteria (lab) can interfere with pathogens through different mechanisms; one is the production of biosurfactants, a group of surface-active molecules, which inhibit the growth of potential pathogens. in the present study, biosurfactants produced by lactobacillus reuteri dsm 17938, lactobacillus acidophilus dds-1, lactobacillus rhamnosus atcc 53103, and lactobacillus paracasei b21060 were dialyzed (1 and 6 kda) and characterized in term of reduction of surface tension and emulsifying ... | 2016 | 27102127 |
| effect of refrigerated storage on probiotic viability and the production and stability of antimutagenic and antioxidant peptides in yogurt supplemented with pineapple peel. | fruit by-products are good resources of carbohydrates, proteins, vitamins, and minerals, which may function as growth nutrients for probiotic bacteria. this research aimed at evaluating effects of pineapple peel powder addition on the viability and activity of lactobacillus acidophilus (atcc 4356), lactobacillus casei (atcc393), and lactobacillus paracasei ssp. paracasei (atcc baa52) in yogurts throughout storage at 4°c for 28d. plain and probiotic yogurts supplemented with or without pineapple ... | 2015 | 26142843 |
| molecular identification and quantification of lactic acid bacteria in traditional fermented dairy foods of russia. | russian traditional fermented dairy foods have been consumed for thousands of years. however, little research has focused on exploiting lactic acid bacteria (lab) resources and analyzing the lab composition of russian traditional fermented dairy foods. in the present study, we cultured lab isolated from fermented mare and cow milks, sour cream, and cheese collected from kalmykiya, buryats, and tuva regions of russia. seven lactobacillus species and the bifidobacterium genus were quantified by qu ... | 2015 | 26004836 |
| the effect of probiotics (lactobacillus rhamnosus hn001, lactobacillus paracasei lpc-37, and lactobacillus acidophilus ncfm) on the availability of minerals from dutch-type cheese. | the use of probiotic cultures in the production of dutch-type cheeses did not lead to significant changes in their chemical composition but it lowered their acidity. the availability of calcium and magnesium analyzed by in vitro enzymatic hydrolysis was 19 and 35%, respectively; the availability of phosphorus was significantly higher, at >90%. the use of probiotic cultures significantly increased the availability of calcium (~2.5%), phosphorus (~6%), and magnesium (~18%). the in vitro method sup ... | 2014 | 24913654 |
| effect of probiotics on antioxidant and antimutagenic activities of crude peptide extract from yogurt. | search for bioactive peptides is intensifying because of the risks associated with the use of synthetic therapeutics, thus peptide liberation by lactic acid bacteria and probiotics has received a great focus. however, proteolytic capacity of these bacteria is strain specific. the study was conducted to establish proteolytic activity of lactobacillus acidophilus (atcc® 4356™), lactobacillus casei (atcc® 393™) and lactobacillus paracasei subsp. paracasei (atcc® baa52™) in yogurt. crude peptides we ... | 2014 | 24629967 |
| comparison of two oral probiotic preparations in a randomized crossover trial highlights a potentially beneficial effect of lactobacillus paracasei ncc2461 in patients with allergic rhinitis. | there is promising but conflicting evidence to recommend the addition of probiotics to foods for prevention and treatment of allergy. based on previous studies with fermented milk containing lactobacillus paracasei ncc2461, we aimed to compare the effect of a powder form of the latter probiotic with the effect of a blend of lactobacillus acidophilus atcc sd5221 and bifidobacterium lactis atcc sd5219 in patients with allergic rhinitis. | 2014 | 24393277 |
| [identification of lactic acid bacteria in commercial yogurt and their antibiotic resistance]. | to identify lactic acid bacteria (lab) in commercial yogurts and investigate their antibiotic resistance. | 2013 | 24341282 |
| effect of a sublethal high-pressure homogenization treatment on the fatty acid membrane composition of probiotic lactobacilli. | high-pressure homogenization (hph) has been proposed to be applied directly to lactic acid bacterial cells at sublethal levels to enhance some functional properties. as the principal target of hph are the cell surface envelope structures, the aim of this work was to study the effect of a hph treatment, applied at 50 mpa, on cell membrane stress responses of already-known functional strains, isolated from argentinean products. specifically, the membrane fatty acid composition of cells before and ... | 2014 | 24111720 |
| long-term treatment with probiotics in primary care patients with irritable bowel syndrome--a randomised, double-blind, placebo controlled trial. | objective. meta-analyses have indicated effect of probiotics on irritable bowel syndrome (ibs). however, few long-term trials have been conducted and uncertainty remains as to effectiveness and long-term effect in a primary care setting. we aimed to investigate the effect of probiotics compared with placebo in the management of ibs in primary care during a 6-month treatment period and with a 6-month follow-up. material and methods. we randomized ibs patients fulfilling rome iii criteria to recei ... | 2013 | 23957590 |
| effects of three-month intake of synbiotic on inflammation and body composition in the elderly: a pilot study. | we hypothesize that improvements in the gut microbiota are capable of ameliorating gut permeability and, consequently, reducing systemic inflammation and the risk of frailty. this study aims to evaluate some effects of synbiotic supplementation on inflammatory markers and the body composition of the elderly at risk of frailty. in a double-blind study that lasted three months, 17 elderly individuals fulfilling one frailty criteria (grip strength) were randomly distributed into two groups: syn (n ... | 2013 | 23595135 |
| factors affecting adhesion of lactic acid bacteria to caco-2 cells and inhibitory effect on infection of salmonella typhimurium. | in this study, seven strains isolated from mustard leaf kimchi were screened for their tolerance to simulated gastric and bile juices, the adhesive properties to caco-2 cells, and the inhibition ability of salmonella typhimurium atcc 29631 adhesion. lactobacillus acidophilus gk20, lactobacillus paracasei gk74, and lactobacillus plantarum gk81, which were resistant to bile as well as gastric juices, possessed high bile-salt hydrolase (bsh) activity towards both sodium glycocholate and sodium taur ... | 2012 | 23221537 |
| sublingual administration of lactobacillus paracasei kw3110 inhibits th2-dependent allergic responses via upregulation of pd-l2 on dendritic cells. | lactic acid bacteria have potential in immunomodulation therapy, but their clinical efficacy and underlying mechanisms are unclear. we aimed to clarify the anti-allergic immune responses induced by intragastric and sublingual administration of heat-killed lactobacillus paracasei kw3110 and lactobacillus acidophilus l-92. the kw3110 strain (but not the l-92 strain) enhanced ovalbumin (ova)-induced expression of ccr-7 and pd-l2 in murine dendritic cells (dcs), and strongly inhibited il-5 and il-13 ... | 2012 | 22386865 |
| identification of lactobacillus strains of goose origin using maldi-tof mass spectrometry and 16s-23s rdna intergenic spacer pcr analysis. | the objective of our study was to identify lactobacillus sp. strains of goose origin using maldi-tof mass spectrometry, its-pcr and its-pcr/rflp. all three techniques proved to be valuable tools for identification of avian lactobacilli and produced comparable classification results. lactobacillus strains were isolated from 100% of geese aged 3 weeks to 4 years, but from only 25% of chicks aged 1-10 days. among the 104 strains isolated, we distinguished 14 lactobacillus species. the dominant spec ... | 2014 | 24607713 |
| naturally occurring lactic acid bacteria isolated from tomato pomace silage. | silage making has become a significant method of forage conservation worldwide. to determine how tomato pomace (tp) may be used effectively as animal feed, it was ensilaged for 90 days and microbiology counts, fermentation characteristics and chemical composition of tomato pomace silage (tps) were evaluated at the 30th, 60th, and 90th days, respectively. in addition, 103 lactic acid bacteria were isolated from tps. based on the phenotypic and chemotaxonomic characteristics, 16s rdna sequence and ... | 2014 | 25049999 |
| probiotic attributes of indigenous lactobacillus spp. isolated from traditional fermented foods and beverages of north-western himalayas using in vitro screening and principal component analysis. | the present research was designed to explore indigenous probiotic lactic acid bacteria from traditional fermented foods and beverages of north-western himalayas for their probiotic potential. it was achieved through a step-by step approach focused on the technological characterization, evaluation of the probiotic traits and adherence ability. fifty one lab isolates from traditional fermented foods and beverages were initially screened for their technological properties and among them twenty isol ... | 2016 | 27407213 |
| lactic acid bacteria in total mixed ration silage containing soybean curd residue: their isolation, identification and ability to inhibit aerobic deterioration. | we investigated the effects of the predominant lactic acid bacteria (lab) on the fermentation characteristics and aerobic stability of total mixed ration (tmr) silage containing soybean curd residue (sc-tmr silage). the sc-tmr materials were ensiled in laboratory silos for 14 or 56 days. lab predominant in sc-tmr silage were identified (exp. 1). lactobacillus fermentum (l. fermentum) and streptococcus bovis (s. bovis) were found in the untreated materials, leuconostoc pseudomesenteroides (l. pse ... | 2016 | 26949952 |
| identification of novel anti-inflammatory probiotic strains isolated from pulque. | probiotics are live microorganisms which when administered in adequate amounts, confer health benefits on the host. their use is more and more widespread for both prevention and treatment of diseases, including traveler’s diarrhea and inflammatory bowel diseases (ibds). in this work, we isolated and characterized novel candidate probiotic strains from pulque (xaxtle), a traditional mexican alcoholic fermented beverage. a total of 14 strains were obtained from xaxtle samples isolated from three d ... | 2016 | 26476654 |