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novel bioactive from lactobacillus brevis dsm17250 to stimulate the growth of staphylococcus epidermidis: a pilot study.commensal skin microbiota plays an important role in both influencing the immune response of the skin and acting as a barrier against colonisation of potentially pathogenic microorganisms and overgrowth of opportunistic pathogens. staphylococcus epidermidis is a key constituent of the normal microbiota on human skin. it balances the inflammatory response after skin injury and produces antimicrobial molecules that selectively inhibit skin pathogens. here we describe lactobacillus brevis dsm17250 ...201727824277
lactobacillus sps. lipase mediated poly (ε-caprolactone) degradation.polymer degradation through lipase appears to be an enthralling alternative to bulk chemical routes. poly (ε-caprolactone) (pcl) is an artificial polyester that can be degraded by microbes and enzymes like lipases and esterases. the environmental degradation of pcl is dependent on the activity of bacteria that characterization techniques such as thermogravimetric analysis, differential thermal are widely present in the ecosystem. in this study, three different lipases derived from lactobacillus ...201727865950
distribution of native lactic acid bacteria in wineries of queretaro, mexico and their resistance to wine-like conditions.native lactic acid bacteria (lab) are capable of growing during winemaking, thereby strongly affecting wine quality. the species of lab present in musts, wines during malolactic fermentation (mlf), and barrels/filters were investigated in wineries from the emerging wine region of queretaro, méxico using multiplex pcr and culture. the resistance to wine-like conditions (wlc): ethanol (10, 12, and 13%), so2 (30 mg⋅l(-1)), and low ph (3.5) of native lab strains was also studied. five species were d ...201627877164
enhanced production of gamma-aminobutyric acid by optimizing culture conditions of lactobacillus brevis hye1 isolated from kimchi, a korean fermented food.this study evaluated the effects of culture conditions, including carbon and nitrogen sources, l-monosodium glutamate (msg), and initial ph, on gamma-aminobutyric acid (gaba) production by lactobacillus brevis hye1 isolated from kimchi, a korean traditional fermented food. l. brevis hye1 was screened by the production analysis of gaba and genetic analysis of the glutamate decarboxylase gene, resulting in 14.64 mm gaba after 48 h of cultivation in mrs medium containing 1% (w/v) msg. in order to i ...201727880963
probiotic supplements beneficially affect tryptophan-kynurenine metabolism and reduce the incidence of upper respiratory tract infections in trained athletes: a randomized, double-blinded, placebo-controlled trial.prolonged intense exercise has been associated with transient suppression of immune function and an increased risk of infections. in this context, the catabolism of amino acid tryptophan via kynurenine may play an important role. the present study examined the effect of a probiotic supplement on the incidence of upper respiratory tract infections (urti) and the metabolism of aromatic amino acids after exhaustive aerobic exercise in trained athletes during three months of winter training.201627886064
monitoring of wheat lactic acid bacteria from the field until the first step of dough fermentation.the present work was carried out to retrieve the origin of lactic acid bacteria (lab) in sourdough. to this purpose, wheat lab were monitored from ear harvest until the first step of fermentation for sourdough development. the influence of the geographical area and variety on lab species/strain composition was also determined. the ears of four triticum durum varieties (duilio, iride, saragolla and simeto) were collected from several fields located within the palermo province (sicily, italy) and ...201727889157
modeling lactic fermentation of gowé using lactobacillus starter culture.a global model of the lactic fermentation step of gowé was developed by assembling blocks hosting models for bacterial growth, lactic acid production, and the drop of ph during fermentation. commercial strains of lactobacillus brevis and of lactobacillus plantarum were used; their growth was modeled using rosso's primary model and the gamma concept as a secondary model. the optimum values of ph and temperature were 8.3 ± 0.3, 44.6 ± 1.2 °c and 8.3 ± 0.3, 3.2 ± 37.1 °c with μmax values of 1.8 ± 0 ...201627916901
characteristics of surface layer proteins from two new and native strains of lactobacillus brevis.in this work, some important characteristics of surface layer (s-layer) proteins extracted from two new and native lactobacillus strains, l.brevis km3 and l.brevis km7, were investigated. the presence of s-layer on the external surface of l.brevis km3 was displayed by thin sectioning and negative staining. sds-page analysis were shown same dominant protein bands approximately around 48kda for both s-layer proteins. moreover, the s-layer reappeared when licl treated cells were allowed to grow aga ...201727984145
adjunctive efficacy of probiotics in the treatment of experimental peri-implant mucositis with mechanical and photodynamic therapy: a randomized, cross-over clinical trial.to evaluate the adjunctive clinical efficacy of probiotics in the treatment of peri-implant mucositis (p-im) with professionally administered plaque removal (papr) and photodynamic therapy (pdt).201728032908
utility of thermostable xylanases of mycothermus thermophilus in generating prebiotic xylooligosaccharides.xylooligosaccharides as emerging prebiotics are able to promote the growth of probiotic bacteria. in the present study, four neutral, thermostable xylanases (mtxyn11a, mtxyn11at, mtxyn11b, and mtxyn11c) from compost fungus mycothermus thermophilus cgmcc3.18119 were overexpressed in pichia pastoris gs115 and used to produce xylooligosaccharides from beechwood xylan. the enzymes showed similar enzymatic properties (maximal activities at ph 6.0-6.5 and 65 °c) but varied in catalytic efficiency and ...201728067041
physiology-oriented engineering strategy to improve gamma-aminobutyrate production in lactobacillus brevis.gamma-aminobutyrate (gaba) is an important chemical in the pharmaceutical field. gaba-producing lactic acid bacteria (lab) offer the opportunity of developing this health-oriented product. in this study, the gada, gadb, gadc, gadcb, and gadca gene segments of lactobacillus brevis were cloned into pmg36e, and strain lb. brevis/pmg36e-gada was selected for thorough characterization in terms of gaba production after analysis of gad activities. subsequently, a physiology-oriented engineering strateg ...201728067044
effects of oral intake of heat-killed lactobacillus brevis sbc8803 (sbl88™) on dry skin conditions: a randomized, double-blind, placebo-controlled study.lactobacilli are important in intestinal homeostasis, which involves the regulation of immune function, digestive health, cholesterol absorption and intestinal tumor growth amongst others. our previous investigations have suggested that oral intake of heat-killed lactobacillus brevis (l. brevis) sbc8803 (sbl88™) suppresses dermatitis by modulating the immune function in an atopic dermatitis mouse model. the aim of the present study was to investigate the effect of heat-killed l. brevis sbc8803 i ...201628105118
probiotic and antioxidant properties of selenium-enriched lactobacillus brevis lse isolated from an iranian traditional dairy product.the present study was designed to isolate a highly selenium-tolerant lactobacillus strain from an iranian traditional dairy product named as spar. different criteria such as tolerance to the low ph, simulated gastric juice (sgj), simulated intestinal juice (sij) and bile salts tolerance as well as caco-2 cell adhesion assay were examined to evaluate the probiotic potentials of the selected isolate. furthermore, the antioxidant properties of the isolate cultivated in medium containing and free of ...201728159215
effect of magnesium acetate on the antimold activity of lactobacillus.the antimold activity of lactic acid bacteria (lab) is used in food biopreservation. the aim of this study was to evaluate the effect of magnesium acetate added to de man rogosa sharpe (mrs) medium on the antimold activity of three lab strains ( lactobacillus plantarum , lactobacillus brevis , and lactobacillus fermentum ) against molds contaminating food ( aspergillus oryzae , aspergillus niger , penicillium chrysogenum , fusarium avenaceum , and rhizopus arrhizus ) and their ability to produce ...201728221876
isolation, identification, and evaluation of new lactic acid bacteria strains with both cellular antioxidant and bile salt hydrolase activities in vitro.in this study, we analyzed chinese traditional fermented food to isolate and identify new lactic acid bacteria (lab) strains with novel functional properties and to evaluate their cellular antioxidant and bile salt hydrolase (bsh) activities in vitro. a sequential screening strategy was developed to efficiently isolate and obtain 261 lab strains tolerant of bile salt, acid, and h2o2 from nine chinese traditional fermented foods. among these strains, 70 were identified as having 2,2-diphenyl-1-pi ...201628221912
evaluation of tyrosinase inhibitory and antioxidant activities of angelica dahurica root extracts for four different probiotic bacteria fermentations.angelica dahurica root (adr), which shows strong antioxidant activity, is used in chinese medicine. this study evaluated the tyrosinase inhibitory and antioxidant activities of adr extracts fermented by four different probiotic bacteria: bifidobacterium bifidum, bifidobacterium lactis, lactobacillus acidophilus, and lactobacillusbrevis. the adr was first extracted using distilled water, 70% ethanol, and ethyl acetate, and then fermented by probiotic bacteria. the physiological characteristics of ...201728254340
common distribution of gad operon in lactobacillus brevis and its gada contributes to efficient gaba synthesis toward cytosolic near-neutral ph.many strains of lactic acid bacteria (lab) and bifidobacteria have exhibited strain-specific capacity to produce γ-aminobutyric acid (gaba) via their glutamic acid decarboxylase (gad) system, which is one of amino acid-dependent acid resistance (ar) systems in bacteria. however, the linkage between bacterial ar and gaba production capacity has not been well established. meanwhile, limited evidence has been provided to the global diversity of gaba-producing lab and bifidobacteria, and their mecha ...201728261168
heterogeneity between and within strains of lactobacillus brevis exposed to beer compounds.this study attempted to investigate the physiological response of six lactobacillus brevis strains to hop stress, with and without the addition of mn(2+) or ethanol. based on the use of different fluorescent probes, cell viability and intracellular ph (phi) were assessed by fluorescence microscopy combined with flow cytometry, at the single cell level. the combined approach was faster than the traditional colony based method, but also provided additional information about population heterogeneit ...201728261191
microbiological and biochemical aspects of inland pecorino abruzzese cheese.little is known on physicochemical and biochemical characteristics of "pecorino" abruzzese cheese in l'aquila province, an artisanal cheese produced from ewe raw full-cream milk. three batches of inland "pecorino" abruzzese cheese were examined for microbiological, compositional, biochemical and sensory characteristics at the aim of isolating and storing in a bacterial collection, indigenous strain to preserve the microbial biodiversity present in this cheese, to a possible definition of a pdo. ...201728280791
metagenomics analysis of microbial communities associated with a traditional rice wine starter culture (xaj-pitha) of assam, india.this is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving illumine-based whole genome shotgun (wgs) sequencing method. metagenomic dna was extracted from rice wine starter culture concocted by ahom community of assam and analyzed using a miseq(®) system. a total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. data obtained from the use of several taxonomic profiling tools were ...201628330225
amelioration of citrobacter rodentium proliferation in early stage of infection in mice by pre-treatment with lactobacillus brevis kb290 and verification using in vivo bioluminescence imaging.bioluminescent imaging (bli) has become a useful tool for monitoring bacterial infections in real time. citrobacter rodentium and its bli are widely used as a murine model of enteropathogenic and enterohaemorrhagic escherichia coli infection. in this study, we evaluated the protective effects of the probiotic lactobacillus brevis kb290 against c. rodentium infection by the bli approach. first, we examined several solutions for making the suspension of bioluminescent c. rodentium for an oral inoc ...201628333177
the γ-aminobutyric acid-producing ability under low ph conditions of lactic acid bacteria isolated from traditional fermented foods of ishikawa prefecture, japan, with a strong ability to produce ace-inhibitory peptides.many traditional fermented products are onsumed in ishikawa prefecture, japan, such as kaburazushi, narezushi, konkazuke, and ishiru. various kinds of lactic acid bacteria (lab) are associated with their fermentation, however, characterization of lab has not yet been elucidated in detail. in this study, we evaluated 53 isolates of lab from various traditional fermented foods by taxonomic classification at the species level by analyzing the 16s ribosomal rna gene (rdna) sequences and carbohydrate ...201628352530
dynamics of microbial community during ensiling direct-cut alfalfa with and without lab inoculant and sugar.to gain deeper insights into the clostridial community dynamics and chemical transformations during the ensiling of alfalfa.201728370869
effect of combined consumption of lactobacillus brevis kb290 and β-carotene on minor diarrhoea-predominant irritable bowel syndrome-like symptoms in healthy subjects: a randomised, double-blind, placebo-controlled, parallel-group trial.people with non-pathological diarrhoea-predominant irritable bowel syndrome (ibs-d)-like symptoms are present at a significant level even in healthy populations, but established prophylaxis is lacking. previously, we have found that co-administration of lactobacillus brevis kb290 (kb290) and β-carotene (βc) attenuated murine colitis (potential cause of ibs-d-like symptoms) significantly. here, we investigated the effect of kb290 and βc on minor ibs-d-like symptoms in healthy volunteers. after a ...201728391736
thermal treatment of lupin-based milk alternatives - impact on lupin proteins and the network of respective lupin-based yogurt alternatives.in this study lupin-based milk alternative (lbma), obtained from protein isolate of lupinus angustifolius cv. boregine, was homogenized and was subjected to different heat treatments (unheated; pasteurized: 80°c, 60s; ultra-high temperature heating (uht): 140°c, 10s). upon thermal treatment, lupin proteins, namely β-conglutin and α-conglutin, were stepwise denatured, which was accompanied by the exposure of masked sulfhydryl groups, the decrease of free sulfhydryl groups and reoxidation to disul ...201628460988
lactic acid bacteria population dynamics during spontaneous fermentation of radish (raphanus sativus l.) roots in brine.the aim of the present study was to assess the microecosystem development and the dynamics of the lactic acid bacteria population during spontaneous fermentation of radish (raphanus sativus l.) roots in brine at 20 and 30 °c. in both temperatures, lactic acid bacteria prevailed the fermentation; as a result, the ph value was reduced to ca. 3.6 and total titrable acidity increased to ca. 0.4% lactic acid. enterococci population increased and formed a secondary microbiota while pseudomonads, enter ...201728466306
three-dimensional structures and functional studies of two gh43 arabinofuranosidases from weissella sp. strain 142 and lactobacillus brevis.arabinofuranosidases degrade arabinose-containing oligo and polysaccharides, releasing l-arabinose, which is a potentially useful sugar, shown to reduce glycemic response under certain conditions. arabinofuranosidases (arafs) are frequently found in gh43, one of the most common gh-families encoded in genomes in gut microbiota, and hence it is of interest to increase understanding of the function of these enzymes in species occurring in the gut. here we have produced, characterized and solved the ...201728485897
lactobacilli-lactoferrin interplay in chlamydia trachomatis infection.in the cervicovaginal microenvironment, lactobacilli are known to protect against genital infections and, amongst the host defence compounds, lactoferrin has recently acquired importance for its anti-microbial and anti-inflammatory properties. an abnormal genital microenvironment facilitates the acquisition of pathogens like chlamydia trachomatis, the leading cause of bacterial sexually transmitted infections worldwide. the aim of our study is to investigate the effects of lactobacillus crispatu ...201728505248
clinical and microbiological effects of the adjunctive use of probiotics in the treatment of gingivitis: a randomized controlled clinical trial.to evaluate the efficacy of a probiotic combination in the treatment of gingivitis and to assess its impact on the subgingival microbiota.201728556062
impact of thistle rennet from carlina acanthifolia all. subsp. acanthifolia on bacterial diversity and dynamics of a specialty italian raw ewes' milk cheese.caciofiore della sibilla is an italian specialty soft cheese manufactured with sopravissana raw ewes' milk and thistle rennet prepared with young fresh leaves and stems of carlina acanthifolia all. subsp. acanthifolia, according to an ancient tradition deeply rooted in the territory of origin (mountainous hinterland of the marche region, central italy). in this study, the impact of thistle rennet on the bacterial dynamics and diversity of caciofiore della sibilla cheese was investigated by apply ...201728558331
effect of ensiling and silage additives on biogas production and microbial community dynamics during anaerobic digestion of switchgrass.silage processing has a crucial positive impact on the methane yield of anaerobic treated substrates. changes in the characteristics of switchgrass after ensiling with different additives and their effects on methane production and microbial community changes during anaerobic digestion were investigated. after ensiling (ck), methane yield was increased by 33.59% relative to that of fresh switchgrass (fs). in comparison with the ck treatment, methane production was improved by 17.41%, 13.08% and ...201728577484
anti-inflammatory potential of a heat-killed lactobacillus strain isolated from kimchi on house dust mite-induced atopic dermatitis in nc/nga mice.atopic dermatitis (ad) is an allergic skin disease driven by the th2-prone immune response. therefore, a fundamental approach to restoring the th1/th2 balance is needed to treat ad.201728632956
development of a propidium monoazide-polymerase chain reaction assay for detection of viable lactobacillus brevis in beer.the spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. the polymerase chain reaction (pcr) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. however, one of the drawbacks is the inability to differentiate between live and dead cells. in this paper, the combination of propidium monoazide (pma) pretreatment and conventional pcr had been describe ...201728633981
multiple genome sequences of exopolysaccharide-producing, brewery-associated lactobacillus brevis strains.lactobacillus brevis represents one of the most relevant beer-spoiling bacteria. besides strains causing turbidity and off flavors upon growth and metabolite formation, this species also comprises strains that produce exopolysaccharides (epss), which increase the viscosity of beer. here, we report the complete genome sequences of three eps-producing, brewery-associated l. brevis strains.201728663299
probiotic-mediated blueberry (vaccinium corymbosum l.) fruit fermentation to yield functionalized products for augmented antibacterial and antioxidant activity.the aim of this study was to investigate the fermentation of blueberry fruit with selected probiotic bacteria (bacillus amyloliquefaciens and lactobacillus brevis) and yeast (starmerella bombicola) isolated from fermented starfish for the extraction of functionalized products for biomedical applications. all probiotic-based fermented extracts showed augmented antibacterial and antioxidant activity compared to the control. biochemical parameters of viable cell count, titratable acidity, total phe ...201728690159
chemoenzymatic synthesis of statine side chain building blocks and application in the total synthesis of the cholesterol-lowering compound solistatin.the synthesis and enzymatic reduction of several 6-substituted dioxohexanoates are presented. two-step syntheses of tert-butyl 6-bromo-3,5-dioxohexanoate and the corresponding 6-hydroxy compound have been achieved in 89% and 59% yield, respectively. regio- and enantioselective reduction of these diketones and of the 6-chloro derivative with alcohol dehydrogenase from lactobacillus brevis (lbadh) gave the (5s)-5-hydroxy-3-oxo products with enantiomeric excesses of 91%, 98.4%, and >99.5%, respecti ...201728751276
complete genome sequence of the sourdough isolate lactobacillus zymae aca-dc 3411.lactobacillus zymae is a gram-positive lactic acid bacterium belonging to the lactobacillus brevis clade. here, we report the first complete genome sequence of l. zymae aca-dc 3411, which was isolated from traditional greek wheat sourdough. whole-genome analysis may reveal adaptive traits of strain aca-dc 3411 in the sourdough ecosystem.201728751400
in vitro evaluation of the probiotic and safety properties of bacteriocinogenic and non-bacteriocinogenic lactic acid bacteria from the intestines of nile tilapia and common carp for their use as probiotics in aquaculture.in this study, seven bacteriocinogenic and non-bacteriocinogenic lab strains previously isolated from the intestines of nile tilapia and common carp and that showed potent antibacterial activity against host-derived and non-host-derived fish pathogens were assayed for their probiotic and safety properties so as to select promising candidates for in vivo application as probiotic in aquaculture. all the strains were investigated for acid and bile tolerances, transit tolerance in simulated gastroin ...201728752423
self-cycling fermentation for 1,3-propanediol production: comparative evaluation of metabolite flux in cell recycling, simple batch and continuous processes using lactobacillus brevis n1e9.3.3 strain.the microbial conversion of biodiesel derived crude glycerol to 1,3-propanediol (1,3-pdo) has attained high industrial value due to the broad range of applications as a monomer (1,3-pdo) in textile, cosmetic and polymer industries. this fine chemical through biological production addressed several limitations of the chemical process like high temperatures, pressure and expensive catalysts. in this study 1,3-propanediol production was achieved from a non-pathogenic lactic acid bacterial strain la ...201728760443
a genetically encoded tool for manipulation of nadp(+)/nadph in living cells.the redox coenzymes nadh and nadph are broadly required for energy metabolism, biosynthesis and detoxification. despite detailed knowledge of specific enzymes and pathways that utilize these coenzymes, a holistic understanding of the regulation and compartmentalization of nadh- and nadph-dependent pathways is lacking, partly because of a lack of tools with which to investigate these processes in living cells. we have previously reported the use of the naturally occurring lactobacillus brevis h2o ...201728805804
correction to: fuke, aizawa, suganuma, takagi, and naito, effect of combined consumption of lactobacillus brevis kb290 and β-carotene on minor diarrhoea-predominant irritable bowel syndrome-like symptoms in healthy subjects: a randomised, double-blind, placebo-controlled, parallel-group trial. 201728817999
microbial production of mannitol by lactobacillus brevis 3-a5 from concentrated extract of jerusalem artichoke tubers.in the present study, the conversion of the extract of jerusalem artichoke tubers for mannitol production by lactobacillus brevis 3-a5 was investigated. when the bacterium utilized enzymatic hydrolysates of jerusalem artichoke extract as the main substrates in batch fermentation, the significant decrease in mannitol productivity was observed when the initial concentration of reducing sugar increased. then, a strategy of continuous fed-batch fermentation was adopted for improving mannitol product ...201728833484
the oxygen-inducible conversion of lactate to acetate in heterofermentative lactobacillus brevis atcc367.lactobacillus brevis is an obligatory heterofermentative lactic acid bacterium that produces high levels of acetate which improves the aerobic stability of silages against deterioration by yeasts and molds. however, the mechanism involved in acetate accumulation has yet to be understood. here, experimental evidence indicated that aerobiosis resulted in the conversion of lactate to acetate after glucose exhaustion in l. brevis atcc367 (genbank accession number nc_008497). to elucidate the convers ...201728842545
lactobacillus brevis cd2 lozenges prevent oral mucositis in patients undergoing high dose chemotherapy followed by haematopoietic stem cell transplantation.oral mucositis is a common inflammatory complication in patients undergoing high-dose chemotherapy and radiation followed by haematopoietic stem cell transplantation (hsct). lactobacillus brevis cd2 has been proven efficacious in preventing chemoradiotherapy-induced oral mucositis in squamous cell carcinoma of head and neck.201628848667
comparison of acyclovir and multistrain lactobacillus brevis in women with recurrent genital herpes infections: a double-blind, randomized, controlled study.we performed a randomized double-blind controlled trial to compare the efficacy and safety of multistrain probiotic and acyclovir in women patients with recurrent genital herpes simplex virus type 2 (hsv-2) infections. eighty-one patients enrolled in the study were being treated with multistrain lactobacillus brevis one vaginal capsule every 12 h and oral acyclovir 400 mg twice daily for 6 months. of 53 patients who completed both treatment courses, no important differences were identified betwe ...201728852985
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