Publications

nutritional properties and antinutritional factors of corn paste (kutukutu) fermented by different strains of lactic acid bacteria.	the aim of this study is to reduce antinutritional factors and to improve the nutritional properties of kutukutu during fermentation with lactic acid bacteria (lab). for that, kutukutu (700 g) was prepared in the laboratory and inoculated with pure cultures of lab (10(9) cfu/ml). then, preparation was incubated for 120 h. every 24 h, kutukutu were collected, dried at 45°c for 24 h, and analyzed. the results showed that lactobacillus brevis g25 increased reducing sugars content to 80.7% in kutuku ...	2015	26904660
genome sequence of rapid beer-spoiling isolate lactobacillus brevis bso 464.	the genome of brewery-isolate lactobacillus brevis bso 464 was sequenced and assembly produced a chromosome and eight plasmids. this bacterium tolerates dissolved co2/pressure and can rapidly spoil packaged beer. this genome is useful for analyzing the genetics associated with beer spoilage by lactic acid bacteria.	2015	26634759
comparison of homo- and heterofermentative lactic acid bacteria for implementation of fermented wheat bran in bread.	despite its potential health benefits, the integration of wheat bran into the food sector is difficult due to several adverse technological and sensory properties such as bitterness and grittiness. sourdough fermentation is a promising strategy to improve the sensory quality of bran without inducing severe changes to the bran matrix. therefore, identification of species/strains with potential for industrial sourdough fermentations is important. we compared the effects of different representative ...	2015	25846933
attenuating effect of lactobacillus brevis g101 on the msg symptom complex in a double-blind, placebo-controlled study.	lactobacillus brevis g101 suppresses the absorption of monosodium glutamate (msg) from the intestine into the blood in mice. therefore, the attenuating effect of orally administered g101 on monosodium glutamate (msg) symptom complex was investigated in humans.	2015	26634058
characterization and mutational analysis of a two-polypeptide bacteriocin produced by citrus iyo-derived lactobacillus brevis 174a.	in the present study, we isolated a lactic acid bacterium (lab) from a citrus iyo fruit and identified it as lactobacillus brevis. this plant-derived lab strain, designated 174a, produces bacteriocin consisting of two polypeptides designated brevicin 174a-β and 174a-γ. although each polypeptide itself displays antibacterial activity, the ability is enhanced 100 fold by mixing both polypeptides at a 1 : 1 ratio. significantly, brevicin 174a inhibits even the growth of several pathogenic bacteria ...	2015	26632181
probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.	probiotics exhibit beneficial functions for host homeostasis maintenance. we herein investigated the mechanism by which lactobacillus brevis-derived poly p exhibited a beneficial function. immunostaining indicated that poly p was captured in the plasma membrane via integrin β1 in caco2/bbe cells. the uptake of poly p was reduced by the inhibition of integrin β1 as well as caveolin-1, a major component of lipid rafts. the function of poly p, including the induction of hsp27 and enhancement of the ...	2015	26459590
lactic acid bacterial starter culture with antioxidant and γ-aminobutyric acid biosynthetic activities isolated from flatfish-sikhae fermentation.	the aim of this study is to select a lactic acid bacterial strain as a starter culture for flatfish-sikhae fermentation and to evaluate its suitability for application in a food system. four strains of lactic acid bacteria isolated from commercial flatfish-sikhae were identified and selected as starter culture candidates through investigation of growth rates, salt tolerance, food safety, and functional properties such as antioxidative and antimicrobial activities. the fermentation properties of ...	2015	26348620
sedative-hypnotic and receptor binding studies of fermented marine organisms.	this study was performed to investigate the sedative-hypnotic activity of γ-aminobutyric acid (gaba)-enriched fermented marine organisms (fmo), including sea tangle (fst) and oyster (fo) by lactobacillus brevis bj20 (l. brevis bj20). fst and fo were tested for their binding activity of the gabaa-benzodiazepine and 5-ht2c receptors, which are well-known molecular targets for sleep aids. we also measured the sleep latency and sleep duration during pentobarbital-induced sleep in mice after oral adm ...	2015	26336589
growth characteristics of lactobacillus brevis kb290 in the presence of bile.	live lactobacillus brevis kb290 have several probiotic activities, including immune stimulation and modulation of intestinal microbial balance. we investigated the adaptation of l. brevis kb290 to bile as a mechanism of intestinal survival. strain kb290 was grown for 5 days at 37 °c in tryptone-yeast extract-glucose (tyg) broth supplemented with 0.5% sodium acetate (tyga) containing 0.15%, 0.3%, or 0.5% bile. growth was determined by absorbance at 620 nm or by dry weight. growth was enhanced as ...	2015	26272823
multi-sensor approach for the monitoring of halitosis treatment via lactobacillus brevis (cd2)-containing lozenges--a randomized, double-blind placebo-controlled clinical trial.	the aim of this randomized clinical trial was to evaluate whether a recently described multi-sensor approach called bionote(®) is accurate enough to verify the efficacy of treatment of patients with halitosis. a treatment with lactobacillus brevis (cd2)-containing lozenges, compared with placebo was tested. the bionote(®) was compared with traditional techniques used to detect halitosis: oralchroma™ and two calibrated odor judges enrolled for the organoleptic assessments. twenty patients (10 tre ...	2015	26266414
the serotonin receptor mediates changes in autonomic neurotransmission and gastrointestinal transit induced by heat-killed lactobacillus brevis sbc8803.	lactobacilli exhibit several health benefits in mammals, including humans. our previous reports established that heat-killed lactobacillus brevis sbc8803 (sbc8803) increased both efferent gastric vagal nerve activity and afferent intestinal vagal nerve activity in rats. we speculated that this strain could be useful for the treatment of gastrointestinal (gi) disorders. in this study, we examined the effects of sbc8803 on peristalsis and the activity of the efferent celiac vagal nerve innervating ...	2015	26259890
dairy streptococcus thermophilus improves cell viability of lactobacillus brevis nps-qw-145 and its γ-aminobutyric acid biosynthesis ability in milk.	most high γ-aminobutyric acid (gaba) producers are lactobacillus brevis of plant origin, which may be not able to ferment milk well due to its poor proteolytic nature as evidenced by the absence of genes encoding extracellular proteinases in its genome. in the present study, two glutamic acid decarboxylase (gad) genes, gada and gadb, were found in high gaba-producing l. brevis nps-qw-145. co-culturing of this organism with conventional dairy starters was carried out to manufacture gaba-rich ferm ...	2015	26245488
antioxidant potential of a unique lab culture isolated from harbin dry sausage: in vitro and in a sausage model.	the lactic acid bacteria pediococcus pentosaceus, lactobacillus curvatus, lactobacillus brevis, and lactobacillus fermentum isolated from harbin dry sausage were evaluated for their potential antioxidant activity. the in vitro results showed that p. pentosaceus had the strongest h2o2 resistance, radical scavenging activity, reducing power, and inhibition of lipid peroxidation (p<0.05). additionally, superoxide dismutase and glutathione peroxidase activities in p. pentosaceus were higher than tho ...	2015	26241464
effects of lactofermented beetroot juice alone or with n-nitroso-n-methylurea on selected metabolic parameters, composition of the microbiota adhering to the gut epithelium and antioxidant status of rats.	an objective of this work was to assess the biological activity of beetroot juice (chrobry variety, beta vulgaris l. ssp. vulgaris), which was lactofermented by probiotic bacteria lactobacillus brevis 0944 and lactobacillus paracasei 0920. the oxidative status of blood serum, kidneys, and liver of rats consuming the fermented beetroot juice were determined. the experimental rats were divided into four groups on diet type: basal diet, basal diet supplemented with fermented beetroot juice, basal d ...	2015	26193312
identification of ecotype-specific marker genes for categorization of beer-spoiling lactobacillus brevis.	the tolerance to hop compounds, which is mainly associated with inhibition of bacterial growth in beer, is a multi-factorial trait. any approaches to predict the physiological differences between beer-spoiling and non-spoiling strains on the basis of a single marker gene are limited. we identified ecotype-specific genes related to the ability to grow in pilsner beer via comparative genome sequencing. the genome sequences of four different strains of lactobacillus brevis were compared, including ...	2015	26187837
inactivation of escherichia coli, saccharomyces cerevisiae, and lactobacillus brevis in low-fat milk by pulsed electric field treatment: a pilot-scale study.	we investigated the effects of a pulsed electric field (pef) treatment on microbial inactivation and the physical properties of low-fat milk. milk inoculated with escherichia coli, saccharomyces cerevisiae, or lactobacillus brevis was supplied to a pilot-scale pef treatment system at a flow rate of 30 l/h. pulses with an electric field strength of 10 kv/cm and a pulse width of 30 μs were applied to the milk with total pulse energies of 50-250 kj/l achieved by varying the pulse frequency. the ina ...	2015	26877640
simultaneous conversion of glucose and xylose to 3-hydroxypropionic acid in engineered escherichia coli by modulation of sugar transport and glycerol synthesis.	escherichia coli expressing the lactobacillus brevis dhab1b2b3 and dhar1r2 clusters and pseudomonas aeruginosa aldhh was engineered to produce 3-hp from glucose and xylose via the glycerol biosynthetic pathway. glycerol, a key precursor for 3-hp biosynthesis was produced by overexpression of the gpd1 and gpp2 genes from saccharomyces cerevisiae. for relief of carbon catabolite repression, deletion of the chromosomal ptsg gene and overexpression of the endogenous xylr gene rendered engineered e. ...	2015	26441028
cu nanoparticles have different impacts in escherichia coli and lactobacillus brevis than their microsized and ionic analogues.	copper formulations have been used for decades for antimicrobial and antifouling applications. with the development of nanoformulations of copper that are more effective than their ionic and microsized analogues, a key regulatory question is whether these materials should be treated as new or existing materials. to address this issue, here we compare the magnitude and mechanisms of toxicity of a series of cu species (at concentration ranging from 2 to 250 μg/ml), including nano cu, nano cuo, nan ...	2015	26168153
construction of a shuttle vector based on the small cryptic plasmid pjy33 from weissella cibaria 33.	a cryptic plasmid, pjy33, from weissella cibaria 33 was characterized. pjy33 was 2365 bp in size with a gc content of 41.27% and contained two putative open reading frames (orfs). orf1 encoded a putative hypothetical protein of 134 amino acids. orf2 was 849 bp in size, and its putative translation product exhibited 87% identity with a replication initiation factor from a plasmid from w. cibaria klc140. a weissella-escherichia coli shuttle vector, pjy33e (6.5 kb, em(r)), was constructed by ligati ...	2015	25882072
lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine mastitis.	bovine mastitis is a costly disease in dairy cattle worldwide. as of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. additional strategies include vaccination and utilization of antibiotics. despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. the goal of this study was to isolate autochthonous lactic acid bacteria (lab) from bovine mammary microbiota that exhibit benefic ...	2015	26713450
a versatile family 3 glycoside hydrolase from bifidobacterium adolescentis hydrolyzes β-glucosides of the fusarium mycotoxins deoxynivalenol, nivalenol, and ht-2 toxin in cereal matrices.	glycosylation plays a central role in plant defense against xenobiotics, including mycotoxins. glucoconjugates of fusarium toxins, such as deoxynivalenol-3-o-β-d-glucoside (don-3g), often cooccur with their parental toxins in cereal-based food and feed. to date, only limited information exists on the occurrence of glucosylated mycotoxins and their toxicological relevance. due to a lack of analytical standards and the requirement of high-end analytical instrumentation for their direct determinati ...	2015	25979885
inhibition of candida albicans adhesion on medical-grade silicone by a lactobacillus-derived biosurfactant.	the study aimed at investigating the ability of biosurfactant (bs) produced by a lactobacillus brevis isolate (cv8lac) to inhibit adhesion and biofilm formation of candida albicans on medical-grade silicone elastomeric disks (seds).	2015	25644534
a randomized controlled trial to test the effect of multispecies probiotics on cognitive reactivity to sad mood.	recent insights into the role of the human microbiota in cognitive and affective functioning have led to the hypothesis that probiotic supplementation may act as an adjuvant strategy to ameliorate or prevent depression.	2015	25862297
structures of folt in substrate-bound and substrate-released conformations reveal a gating mechanism for ecf transporters.	energy-coupling factor (ecf) transporters are a new family of abc transporters that consist of four subunits, two cytoplasmic atpases ecfa and ecfa' and two transmembrane proteins namely ecfs for substrate-specific binding and ecft for energy coupling. here, we report the 3.2-å resolution crystal structure of the ecfs protein of a folate ecf transporter from enterococcus faecalis-effolt, a close homologue of folt from lactobacillus brevis-lbfolt. structural and biochemical analyses reveal the re ...	2015	26198469
γ-aminobutyric acid (gaba) production and angiotensin-i converting enzyme (ace) inhibitory activity of fermented soybean containing sea tangle by the co-culture of lactobacillus brevis with aspergillus oryzae.	to enhance the γ-aminobutyric acid (gaba) content, the optimized fermentation of soybean with added sea tangle extract was evaluated at 30°c and ph 5.0. the medium was first inoculated with aspergillus oryzae strain fmb s46471 and fermented for 3 days, followed by the subsequent inoculation with lactobacillus brevis gaba 100. after fermentation for 7 days, the fermented soybean showed approximately 1.9 g/kg gaba and exhibited higher ace inhibitory activity than the traditional soybean product. f ...	2015	25876604
microbiological and chemical characteristics of tarubá, an indigenous beverage produced from solid cassava fermentation.	the aim of this work was to identify and characterize the microbiota present during fermentation and in the final beverage, tarubá, by culture-dependent and -independent methods. in addition, target chemical compounds (carbohydrates, organic acids, and ethanol) were evaluated. lactic acid bacteria (lab) and mesophilic bacteria were the predominant microorganisms. among them, lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides, and bacillus subtilis were frequently isolated a ...	2015	25846929
in vitro digestion and fermentation properties of linear sugar-beet arabinan and its oligosaccharides.	this study was conducted to investigate the prebiotic effects of linear arabino-oligosaccharides (laos) and debranched (linear) sugar beet arabinan (lar) for the development of new prebiotics. laos were prepared from lar by enzymatic hydrolysis with endo-arabinanase from bacillus licheniformis, followed by removal of the arabinose fraction by incubation with resting cells of leuconostoc mesenteroides. the resulting laos contained dp2 (28.7%), dp3 (49.9%), dp4 (20.1%), and dp5 (1.16%). a standard ...	2015	26256159
phenotypic and genotypic identification of lactic acid bacteria isolated from traditional pickles of the çubuk region in turkey.	a total of 152 lactic acid bacteria (lab) were isolated from pickles produced in the ankara-çubuk region. these isolates were clustered into eight groups on the basis of their phenotypic characteristics including cell morphology, co2 production from glucose, growth at 10 and 45 °c, growth in 6.5 % nacl, and growth at ph 9.6. api 50 ch carbohydrate fermentation test, 16s ribosomal rna (rrna) sequence analysis, and sodium dodecyl sulfate-acrylamide gel electrophoresis (sds-page) whole-cell protein ...	2015	25404550
metabolic engineering of klebsiella pneumoniae for the production of 2-butanone from glucose.	2-butanone is an important commodity chemical of wide application in different areas. in this study, klebsiella pneumoniae was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. to identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydratases. by introducing the diol dehydratase from lactobacillus brevis and deleting the ldha gene encodin ...	2015	26465746
inhibitory activity in vitro of probiotic lactobacilli against oral candida under different fermentation conditions.	clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral candida species. sugar utilisation by both lactobacilli and candida was also assessed. agar overlay assay was utilised to study growth inhibition of candida albicans, candida glabrata and candida krusei by lactobacillus rhamnosus gg, ...	2015	25380800
characterization of the production of biogenic amines and gamma-aminobutyric acid in the soybean pastes fermented by aspergillus oryzae and lactobacillus brevis.	the production of gamma-aminobutyric acid (gaba) using gaba-producing lactic acid bacteria (lab) has been considered to be an attractive strategy. however, some lab may produce biogenic amines (ba), which may be of concern from the safety viewpoint. the aim of the present study was to characterize the production of gaba and ba in the soybean pastes fermented by aspergillus oryzae (a. oryzae) fmb s46471 and gaba-producing lactobacillus brevis (l. brevis) gaba 100. after a ripening period of 90 da ...	2015	25341471
coexpression of lactobacillus brevis adh with gdh or g6pdh in arxula adeninivorans for the synthesis of 1-(r)-phenylethanol.	the yeast arxula adeninivorans was used for the overexpression of an adh gene of lactobacillus brevis coding for (r)-specific alcohol dehydrogenase (lbadh) to synthesise enantiomerically pure 1-(r)-phenylethanol. glucose dehydrogenase gene from bacillus megaterium (bmgdh) or glucose 6-phosphate dehydrogenase of bacillus pumilus (bpg6pdh) were coexpressed in arxula to regenerate the cofactor nadph by oxidising glucose or glucose 6-phosphate. the yeast strain expressing lbadh and bpg6pdh produced ...	2015	25535093
anti-bacterial and anti-biofilm activity of probiotic bacteria against oral pathogens.	in this study, three lactic acid bacteria (lab), isolated from barley, traditional dried meat and fermented olive were characterized and tested for their anti-bacterial and anti-biofilm activities against oral bacteria. our results revealed that the tested lab were γ-hemolytic and were susceptible to four antibiotics. all the strains were resistant to low ph, bile salt, pepsin and pancreatin. furthermore, fb2 displayed a high aut-oaggregative phenotype (99.54%) while ff2 exhibited the best co-ag ...	2016	27317856
biological control of afb1-producing aspergillus section flavi strains isolated from brewer's grains, alternative feed intended for swine production in argentina.	the aim of the present study was to investigate the inhibitory activity of lactic acid bacteria (lab) isolated from brewer's grains on aspergillus section flavi growth and aflatoxin b1 production. the aspergillus strains tested were inhibited by all the lab strains assayed. the isolates lactobacillus brevis b20, p. pentosaceus b86, lactococcus lactis subsp. lactis b87, l. brevis b131, and lactobacillus sp. b144 completely suppressed the fungal growth and reduced aflatoxin b1 production. in concl ...	2016	27070819
new insight into microbial diversity and functions in traditional vietnamese alcoholic fermentation.	the roles of microorganisms in traditional alcoholic fermentation are often assumed based on abundance in the starter and activity in pure culture. there is a serious lack of hard evidence on the behavior and activity of individual microbial species during the actual fermentation process. in this study, microbial succession and metabolite changes during 7days of traditional vietnamese alcoholic fermentation were monitored. special attention was devoted to starch degradation. in total, 22 microbi ...	2016	27236224
activation of the nrf2 cell defense pathway by ancient foods: disease prevention by important molecules and microbes lost from the modern western diet.	the nrf2 (nfe2l2) cell defense pathway protects against oxidative stress and disorders including cancer and neurodegeneration. although activated modestly by oxidative stress alone, robust activation of the nrf2 defense mechanism requires the additional presence of co-factors that facilitate electron exchange. various molecules exhibit this co-factor function, including sulforaphane from cruciferous vegetables. however, natural co-factors that are potent and widely available from dietary sources ...	2016	26885667
elucidating and regulating the acetoin production role of microbial functional groups in multispecies acetic acid fermentation.	acetoin (3-hydroxy-2-butanone) formation in vinegar microbiota is crucial for the flavor quality of zhenjiang aromatic vinegar, a traditional vinegar produced from cereals. however, the specific microorganisms responsible for acetoin formation in this centuries-long repeated batch fermentation have not yet been clearly identified. here, the microbial distribution discrepancy in the diacetyl/acetoin metabolic pathway of vinegar microbiota was revealed at the species level by a combination of meta ...	2016	27451452
a prebiotic role of ecklonia cava improves the mortality of edwardsiella tarda-infected zebrafish models via regulating the growth of lactic acid bacteria and pathogen bacteria.	in this study, the beneficial prebiotic roles of ecklonia cava (e. cava, ec) were evaluated on the growth of lactic acid bacteria (lab) and pathogen bacteria and the mortality of pathogen-bacteria infected zebrafish model. the result showed that the original e. cava (ec) led to the highest growth effects on three labs (lactobacillus brevis, l. brevis; lactobacillus pentosus, l. pentosus; lactobacillus plantarum; l. plantarum) and it was dose-dependent manners. also, ec, its celluclast enzymatic ...	2016	27192145
combination of xylanase and debranching enzymes specific to wheat arabinoxylan improve the growth performance and gut health of broilers.	arabinoxylan (ax) is the major antinutritional factor of wheat. this study evaluated the synergistic effects of xylanase and debranching enzymes (arabinofuranosidase [abf] and feruloyl esterase [fae]) on ax. during in vitro tests, the addition of abf or fae accelerated the hydrolysis of water-soluble ax (we-ax) and water-insoluble ax (wu-ax) and produced more xylan oligosaccharides (xos) than xylanase alone. xos obtained from we-ax stimulated greater proliferation of lactobacillus brevis and bac ...	2016	27285356
isolation, identification, and evaluation of new lactic acid bacteria strains with both cellular antioxidant and bile salt hydrolase activities in vitro.	in this study, we analyzed chinese traditional fermented food to isolate and identify new lactic acid bacteria (lab) strains with novel functional properties and to evaluate their cellular antioxidant and bile salt hydrolase (bsh) activities in vitro. a sequential screening strategy was developed to efficiently isolate and obtain 261 lab strains tolerant of bile salt, acid, and h2o2 from nine chinese traditional fermented foods. among these strains, 70 were identified as having 2,2-diphenyl-1-pi ...	2016	28221912
microbiological characterization of traditional dough fermentation starter (jiaozi) for steamed bread making by culture-dependent and culture-independent methods.	in this study, the microbial composition of two types of jiaozi (a dough fermentation starter in making steamed bread) was investigated using both culture-dependent and culture-independent (pcr-dgge) methods. the numbers of the cultivable bacteria on mrs at 30°c and yeast on ypd at 28°c in the maize flour jiaozi (mfj) were 9.21±0.16 log cfu/g and 9.18±0.05 log cfu/g, respectively, which were higher than that in the rice flour jiaozi (rfj) (p<0.05). a total of 140 bacteria and 124 yeasts were iso ...	2016	27351835
lactic acid microbiota identification in water, raw milk, endogenous starter culture, and fresh minas artisanal cheese from the campo das vertentes region of brazil during the dry and rainy seasons.	minas artisanal cheese, produced in the campo das vertentes region of brazil, is made from raw milk and endogenous starter cultures. although this cheese is of great historical and socioeconomic importance, little information is available about its microbiological and physical-chemical qualities, or about its beneficial microbiota. this work was aimed at evaluating the qualities of the cheese and the components used for its production, comparing samples collected during the dry and rainy seasons ...	2016	27289151
complementation of mitochondrial electron transport chain by manipulation of the nad+/nadh ratio.	a decline in electron transport chain (etc) activity is associated with many human diseases. although diminished mitochondrial adenosine triphosphate production is recognized as a source of pathology, the contribution of the associated reduction in the ratio of the amount of oxidized nicotinamide adenine dinucleotide (nad(+)) to that of its reduced form (nadh) is less clear. we used a water-forming nadh oxidase from lactobacillus brevis (lbnox) as a genetic tool for inducing a compartment-specif ...	2016	27124460
improvement of the antifungal activity of lactic acid bacteria by addition to the growth medium of phenylpyruvic acid, a precursor of phenyllactic acid.	the aim of the current study was to improve the antifungal activity of eight lactic acid bacterial (lab) strains by the addition of phenylpyruvic acid (ppa), a precursor of the antifungal compound phenyllactic acid (pla), to a defined growth medium (dm). the effect of ppa addition on the labs antifungal activity related to the production of organic acids (pla, d-lactic, l-lactic, acetic, citric, formic and 4-hydroxy-phenyllactic acids) and of other phenylpyruvic-derived molecules, was investigat ...	2016	26827290
metagenomics analysis of microbial communities associated with a traditional rice wine starter culture (xaj-pitha) of assam, india.	this is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving illumine-based whole genome shotgun (wgs) sequencing method. metagenomic dna was extracted from rice wine starter culture concocted by ahom community of assam and analyzed using a miseq(®) system. a total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. data obtained from the use of several taxonomic profiling tools were ...	2016	28330225
three-step biocatalytic reaction using whole cells for efficient production of tyramine from keratin acid hydrolysis wastewater.	tyramine has been paid more attention in recent years as a significant metabolite of tyrosine and catecholamine drug and an intermediate of medicinal material and some drugs. in this study, an effective, green, and three-step biocatalytic synthesis method for production of tyramine starting from serine in keratin acid hydrolysis wastewater was developed and investigated. serine deaminase from escherichia coli was first combined with tyrosine phenol-lyase from citrobacter koseri, to convert l-ser ...	2016	26476652
amelioration of citrobacter rodentium proliferation in early stage of infection in mice by pre-treatment with lactobacillus brevis kb290 and verification using in vivo bioluminescence imaging.	bioluminescent imaging (bli) has become a useful tool for monitoring bacterial infections in real time. citrobacter rodentium and its bli are widely used as a murine model of enteropathogenic and enterohaemorrhagic escherichia coli infection. in this study, we evaluated the protective effects of the probiotic lactobacillus brevis kb290 against c. rodentium infection by the bli approach. first, we examined several solutions for making the suspension of bioluminescent c. rodentium for an oral inoc ...	2016	28333177
isolation, selection and evaluation of antagonistic yeasts and lactic acid bacteria against ochratoxigenic fungus aspergillus westerdijkiae on coffee beans.	in this study, yeasts and lactic acid bacteria (lab) were isolated from coffee fruits and identified via biochemical and molecular approaches. the isolates represented the pichia, debaryomyces, candida, clavispora, yarrowia, sporobolomyces, klyveromyces, torulaspora and lactobacillus genera. four isolates, namely pichia fermentans lpbyb13, sporobolomyces roseus lpby7e, candida sp. lpby11b and lactobacillus brevis lpbb03, were found to have the greatest antagonist activity against an ochratoxigen ...	2016	26544541
comparative evaluation of the genomes of three common drosophila-associated bacteria.	drosophila melanogaster is an excellent model to explore the molecular exchanges that occur between an animal intestine and associated microbes. previous studies in drosophila uncovered a sophisticated web of host responses to intestinal bacteria. the outcomes of these responses define critical events in the host, such as the establishment of immune responses, access to nutrients, and the rate of larval development. despite our steady march towards illuminating the host machinery that responds t ...	2016	27493201
probiotic supplements beneficially affect tryptophan-kynurenine metabolism and reduce the incidence of upper respiratory tract infections in trained athletes: a randomized, double-blinded, placebo-controlled trial.	prolonged intense exercise has been associated with transient suppression of immune function and an increased risk of infections. in this context, the catabolism of amino acid tryptophan via kynurenine may play an important role. the present study examined the effect of a probiotic supplement on the incidence of upper respiratory tract infections (urti) and the metabolism of aromatic amino acids after exhaustive aerobic exercise in trained athletes during three months of winter training.	2016	27886064
biofilm-forming capacity in biogenic amine-producing bacteria isolated from dairy products.	biofilms on the surface of food industry equipment are reservoirs of potentially food-contaminating bacteria-both spoilage and pathogenic. however, the capacity of biogenic amine (ba)-producers to form biofilms has remained largely unexamined. bas are low molecular weight, biologically active compounds that in food can reach concentrations high enough to be a toxicological hazard. fermented foods, especially some types of cheese, accumulate the highest ba concentrations of all. the present work ...	2016	27242675
microbiological characterization using combined culture dependent and independent approaches of casizolu pasta filata cheese.	casizolu is a traditional sardinian (italy) pasta filata cheese made with cow raw milk belonging to sardo-modicana and/or bruno-sarda breeds added with natural whey starter. this work aims to describe the traditional technology of this product and to evaluate the microbial groups/species involved in the first month of ripening.	2016	26551888
dispensability of the [4fe-4s] cluster in novel homologues of adenine glycosylase muty.	7,8-dihydro-8-deoxyguanine (8og) is one of the most common oxidative lesions in dna. dna polymerases misincorporate an adenine across from this lesion. thus, 8og is a highly mutagenic lesion responsible for g:c→t:a transversions. muty is an adenine glycosylase, part of the base excision repair pathway that removes adenines, when mispaired with 8og or guanine. its catalytic domain includes a [4fe-4s] cluster motif coordinated by cysteinyl ligands. when this cluster is absent, muty activity is dep ...	2016	26613369
cloning and characterization of two distinct water-forming nadh oxidases from lactobacillus pentosus for the regeneration of nad.	two uncharacterized nicotinamide adenine dinucleotide (nadh) oxidases (named as lpnox1, lpnox2) from lactobacillus pentosus atcc 8041 were cloned and overexpressed in escherichia coli bl21 (de3). the sequence analysis revealed that the two enzymes are water-forming noxs with 64 % and 52 % identity to lbnox from lactobacillus brevis dsm 20054. the optimal ph and temperature of the purified lpnox1 and lpnox2 were 7.0 and 8.0 and 35 and 40 °c, respectively, with k m of 99.0 μm (lpnox1) and 27.6 μm ...	2016	26801669
anti-inflammaging effects of lactobacillus brevis ow38 in aged mice.	in the present study, lactic acid bacteria (lab) strains were collected from kimchi and were screened to isolate strains that inhibit lipopolysaccharide (lps) production by escherichia coli and p16 expression and nuclear factor-kappa b (nf-κb) activation in lps-stimulated macrophages. oral administration of lactobacillus brevis ow38 (1×10(9) cfu/mouse) to aged mice (male, 18 months old) for 8 weeks reduced the lps level in colon fluid and blood. in addition, ow38 treatment also reduced the ratio ...	2016	27824273
cloning and characterization of ginsenoside-hydrolyzing β-glucosidase from lactobacillus brevis that transforms ginsenosides rb1 and f2 into ginsenoside rd and compound k.	the ginsenoside-hydrolyzing β-glucosidase gene (bgy2) was cloned from lactobacillus brevis. we expressed this gene in escherichia coli bl21(de3), isolated the resulting protein, and then utilized the enzyme for the biotransformation of ginsenosides. the bgy2 gene contains 2,223 bp, and encodes a protein of 741 amino acids that is a member of glycosyl hydrolase family 3. β-glucosidase (bgy2) cleaved the outer glucose moieties of ginsenosides at the c-20 position, and the inner glucose at the c-3 ...	2016	27435543
a whole cell biocatalyst for double oxidation of cyclooctane.	a novel whole cell cascade for double oxidation of cyclooctane to cyclooctanone was developed. the one-pot oxidation cascade requires only a minimum of reaction components: resting e. coli cells in aqueous buffered medium (=catalyst), the target substrate and oxygen as environmental friendly oxidant. conversion of cyclooctane was catalysed with high efficiency (50% yield) and excellent selectivity (>94%) to cyclooctanone. the reported oxidation cascade represents a novel whole cell system for do ...	2016	27771781
efficient (3r)-acetoin production from meso-2,3-butanediol using a new whole-cell biocatalyst with co-expression of meso-2,3-butanediol dehydrogenase, nadh oxidase and vitreoscilla hemoglobin.	acetoin (ac) is a volatile platform compound with various potential industrial applications. ac contains two stereoisomeric forms: (3s)-ac and (3r)-ac. optically pure ac is important potential intermediate and widely used as a precursor to synthesize novel optically materials. in this study, chiral (3r)-ac production from meso-2,3-butanediol (meso-2,3-bd) was obtained using recombinant escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-bdh), nadh oxidase (nox) and h ...	2016	27713210
biotransformation of gypenoside xvii to compound k by a recombinant β-glucosidase.	to study the β-glucosidase gene (bgy1) from lactobacillus brevis that was cloned and expressed in escherichia coli bl21 (de3) and then using it for the biotransformation of gypenoside xvii.	2016	27060008
s-layer proteins from lactobacillus sp. inhibit bacterial infection by blockage of dc-sign cell receptor.	many species of lactobacillus sp. possess surface(s) layer proteins in their envelope. among other important characteristics s-layer from lactobacillus acidophilus binds to the cellular receptor dc-sign (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin; cd209), which is involved in adhesion and infection of several families of bacteria. in this report we investigate the activity of new s-layer proteins from the lactobacillus family (lactobacillus acidophilus, lacto ...	2016	27498415
expression and characterization of a novel 1,3-propanediol dehydrogenase from lactobacillus brevis.	1,3-propanediol dehydrogenase (pdor) is important in the biosynthesis of 1,3-propanediol. in the present study, the dhat gene encoding pdor was cloned from lactobacillus brevis 6239 and expressed in escherichia coli for the first time. sequence analysis revealed that pdor containing two fe(2+)-binding motifs and a cofactor motif belongs to the type iii alcohol dehydrogenase. the purified recombinant pdor exhibited a single band of 42 kda according to sds-page. optimal temperatures and ph values ...	2016	26961082
identification of lactobacillus brevis using a species-specific aflp-derived marker.	a simple and specific method for the rapid detection and identification of lactobacillus brevis was developed. a faflp (fluorescent amplified fragment length polymorphisms) marker for l. brevis was used to design oligonucleotide primers for a species-specific pcr assay, targeting a 125bp fragment of the gene encoding the aldo/keto reductase of the diketogulonate-reductase family of l. brevis. this assay resulted in 100% inclusivity and exclusivity of assignment of strains to the species l. brevi ...	2016	27289191
metagenomic sequencing reveals the relationship between microbiota composition and quality of chinese rice wine.	chinese rice wine (crw) is a common alcoholic beverage in china. to investigate the influence of microbial composition on the quality of crw, high throughput sequencing was performed for 110 wine samples on bacterial 16s rrna gene and fungal internal transcribed spacer ii (its2). bioinformatic analyses demonstrated that the quality of yeast starter and final wine correlated with microbial taxonomic composition, which was exemplified by our finding that wine spoilage resulted from a high proporti ...	2016	27241862
the γ-aminobutyric acid-producing ability under low ph conditions of lactic acid bacteria isolated from traditional fermented foods of ishikawa prefecture, japan, with a strong ability to produce ace-inhibitory peptides.	many traditional fermented products are onsumed in ishikawa prefecture, japan, such as kaburazushi, narezushi, konkazuke, and ishiru. various kinds of lactic acid bacteria (lab) are associated with their fermentation, however, characterization of lab has not yet been elucidated in detail. in this study, we evaluated 53 isolates of lab from various traditional fermented foods by taxonomic classification at the species level by analyzing the 16s ribosomal rna gene (rdna) sequences and carbohydrate ...	2016	28352530
electrochemical sandwich assay for attomole analysis of dna and rna from beer spoilage bacteria lactobacillus brevis.	attomole (10(-18)mol) levels of rna and dna isolated from beer spoilage bacterial cells lactobacillus brevis have been detected by the electrochemical sandwich dna hybridization assay exploiting enzymatic activity of lipase. dna sequences specific exclusively to l. brevis dna and rna were selected and used for probe and target dna design. the assay employs magnetic beads (mb) modified with a capture dna sequence and a reporter dna probe labeled with the enzyme, both made to be highly specific fo ...	2016	22633494
antifungal sourdough lactic acid bacteria as biopreservation tool in quinoa and rice bread.	the use of sourdough fermented with specific strains of antifungal lactic acid bacteria can reduce chemical preservatives in bakery products. the main objective of this study was to investigate the production of antifungal carboxylic acids after sourdough fermentation of quinoa and rice flour using the antifungal strains lactobacillus reuteri r29 and lactobacillus brevis r2δ as bioprotective cultures and the non-antifungal l. brevis l1105 as a negative control strain. the impact of the fermentat ...	2016	27236463
contribution of the activated catalase to oxidative stress resistance and γ-aminobutyric acid production in lactobacillus brevis.	lactic acid bacteria (lab) are generally sensitive to h2o2, a compound which can paradoxically produce themselves and lead to the growth arrest and cell death. to counteract the potentially toxic effects of this compound, the gene kate encoding a heme-dependent catalase (cat) belonging to the family of monofunctional cats was cloned from lactobacillus brevis cgmcc1306. the enhanced homologous cat expression was achieved using the nice system. l. brevis cells with overexpressed cat showed 685-fol ...	2016	27716473
metabolic flux analysis of carbon balance in lactobacillus strains.	metabolic flux analyses were performed based on the carbon balance of six different lactobacillus strains used in this study. results confirmed that l. delbrueckii, l. plantarum atcc 21028, l. plantarum ncimb 8826 δldhl1, l. plantarum ncimb 8826 δldhl1-pcu-pxylab, and l. plantarum ncimb 8826 δldhl1-plem415-xylab metabolized glucose via emp: whereas, l. brevis metabolized glucose via pk pathway. xylose was metabolized through the pk pathway in l. brevis, l. plantarum ncimb 8826 δldhl1-pcu-pxylab ...	2016	27602554
anti-atherosclerotic effect of traditional fermented cheese whey in atherosclerotic rabbits and identification of probiotics.	traditional fermented cheese whey (tfcw), containing probiotics, has been used both as a dairy food with ethnic flavor and a medicine for cardiovascular disease, especially regulating blood lipid among kazakh. we therefore investigated anti-atherosclerotic effects of tfcw in atherosclerotic rabbits and identified lactic acid bacteria (lab) and yeasts in tfcw.	2016	27553960
[enhancing glutamate decarboxylase activity by site-directed mutagenesis: an insight from ramachandran plot].	glutamate decarboxylase (gad) can catalyze the decarboxylation of glutamate into γ-aminobutyrate (gaba) and is the only enzyme of gaba biosynthesis. improving gad activity and thermostability will be helpful for the highly efficient biosynthesis of gaba. according to the ramachandran plot information of gad 1407 three-dimensional structure from lactobacillus brevis cgmcc no. 1306, we identified the unstable site k413 as the mutation target, constructed the mutant gad by site-directed mutagenesis ...	2016	27443004
probiotic attributes of indigenous lactobacillus spp. isolated from traditional fermented foods and beverages of north-western himalayas using in vitro screening and principal component analysis.	the present research was designed to explore indigenous probiotic lactic acid bacteria from traditional fermented foods and beverages of north-western himalayas for their probiotic potential. it was achieved through a step-by step approach focused on the technological characterization, evaluation of the probiotic traits and adherence ability. fifty one lab isolates from traditional fermented foods and beverages were initially screened for their technological properties and among them twenty isol ...	2016	27407213
transcriptome analysis of beer-spoiling lactobacillus brevis bso 464 during growth in degassed and gassed beer.	lactobacillus brevis bso 464 (lb464) is a beer-spoilage-related (bsr) isolate of interest given its unique physiological attributes; specifically, it is highly hop-tolerant and exhibits very rapid growth in pressurized/gassed beer. rna sequencing was performed on lb464 grown in pressurized and non-pressurized beer to determine important genetic mechanisms for growth in these environments. the data generated were compared against data in a previous transcriptional study of another lactic acid bac ...	2016	27394184
microbial diversity of type i sourdoughs prepared and back-slopped with wholemeal and refined soft (triticum aestivum) wheat flours.	the fermentation of type i sourdough was studied for 20 d with daily back-slopping under laboratory and artisan bakery conditions using 1 wholemeal and 2 refined soft wheat (triticum aestivum) flours. the sourdough bacterial and yeast diversity and dynamics were investigated by plate counting and a combination of culture-dependent and culture-independent pcr-dgge approach. the ph, total titrable acidity, and concentration of key organic acids (phytic, lactic, and acetic) were measured. three flo ...	2016	27332783
crystal structure of tyrosine decarboxylase and identification of key residues involved in conformational swing and substrate binding.	tyrosine decarboxylase (tdc) is a pyridoxal 5-phosphate (plp)-dependent enzyme and is mainly responsible for the synthesis of tyramine, an important biogenic amine. in this study, the crystal structures of the apo and holo forms of lactobacillus brevis tdc (lbtdc) were determined. the lbtdc displays only 25% sequence identity with the only reported tdc structure. site-directed mutagenesis of the conformationally flexible sites and catalytic center was performed to investigate the potential catal ...	2016	27292129
modeling lactic fermentation of gowé using lactobacillus starter culture.	a global model of the lactic fermentation step of gowé was developed by assembling blocks hosting models for bacterial growth, lactic acid production, and the drop of ph during fermentation. commercial strains of lactobacillus brevis and of lactobacillus plantarum were used; their growth was modeled using rosso's primary model and the gamma concept as a secondary model. the optimum values of ph and temperature were 8.3 ± 0.3, 44.6 ± 1.2 °c and 8.3 ± 0.3, 3.2 ± 37.1 °c with μmax values of 1.8 ± 0 ...	2016	27916901
effects of oral intake of heat-killed lactobacillus brevis sbc8803 (sbl88™) on dry skin conditions: a randomized, double-blind, placebo-controlled study.	lactobacilli are important in intestinal homeostasis, which involves the regulation of immune function, digestive health, cholesterol absorption and intestinal tumor growth amongst others. our previous investigations have suggested that oral intake of heat-killed lactobacillus brevis (l. brevis) sbc8803 (sbl88™) suppresses dermatitis by modulating the immune function in an atopic dermatitis mouse model. the aim of the present study was to investigate the effect of heat-killed l. brevis sbc8803 i ...	2016	28105118
distribution of native lactic acid bacteria in wineries of queretaro, mexico and their resistance to wine-like conditions.	native lactic acid bacteria (lab) are capable of growing during winemaking, thereby strongly affecting wine quality. the species of lab present in musts, wines during malolactic fermentation (mlf), and barrels/filters were investigated in wineries from the emerging wine region of queretaro, méxico using multiplex pcr and culture. the resistance to wine-like conditions (wlc): ethanol (10, 12, and 13%), so2 (30 mg⋅l(-1)), and low ph (3.5) of native lab strains was also studied. five species were d ...	2016	27877164
use of autochthonous lactic acid bacteria starters to ferment mango juice for promoting its probiotic roles.	strains of leuconostoc mesenteroides, pediococcus pentosaceus, and lactobacillus brevis were identified from mango fruits by partial 16s rdna gene sequence. based on the ability of producing mannitol and diacetyl, leuconostoc mesenteroides mpl18 and mpl39 were selected within the lactic acid bacteria isolates, and used as mixed starters to ferment mango juice (mj). both the autochthonous strains grew well in fermented mango juice (fmj) and remained viable at 9.81 log cfu ml(-1) during 30 days of ...	2016	26176886
β-glucan as an encapsulating agent: effect on probiotic survival in simulated gastrointestinal tract.	three strains of probiotics lactobacillus casei, lactobacillus brevis, and lactobacillus plantarum were encapsulated in β-glucan matrix using emulsion technique. further the encapsulated cells were studied for their tolerance in simulated gastrointestinal conditions and its storage stability. the average encapsulation efficiency of β-glucan-probiotic beads was found to be 74.01%. the surface morphology of β-glucan containing bacteria was studied using sem. the noteworthy absorptions in the ft-ir ...	2016	26562556
identification of lactic acid bacteria isolated from wine using real-time pcr.	different lactic acid bacteria strains have been shown to cause wine spoilage, including the generation of substances undesirable for the health of wine consumers. the aim of this study was to investigate the occurrence of selected species of heterofermentative lactobacilli, specifically lactobacillus brevis, lactobacillus hilgardii, and lactobacillus plantarum in six different slovak red wines following the fermentation process. in order to identify the dominant lactobacillus strain using quant ...	2016	26549195
acid production and conversion of konjac glucomannan during in vitro colonic fermentation affected by exogenous microorganisms and tea polyphenols.	impacts of exogenous microorganisms and tea polyphenols on acid production and conversion during in vitro colonic fermentation of konjac glucomannan (kgm) were assessed in this study. colonic fermentation of kgm by the fecal extract of healthy adults resulted in a propionate-rich profile, as acetic, propionic, butyric and lactic acids production were 16.1, 13.0, 3.3 and 20.2 mmol/l, respectively. inoculation of one of ten exogenous microorganisms in the fermentative systems increased acetic, pro ...	2016	26902110
uses of miscanthus press juice within a green biorefinery platform.	this study assesses some uses of nutrient-rich juice mechanically extracted from freshly harvested miscanthus x giganteus (mxg) as part of a green biorefinery system. the juice was used for culturing saccharomyces cerevisiae and lactic acid bacteria. mxg juice was further used as substrate for fermentation to produce lactic acid using lactobacillus brevis and lactobacillus plantarum. the results show that mxg juice was a highly nutritious source for the cultivation of bacteria. higher concentrat ...	2016	26896712
identification of beer spoilage microorganisms using the maldi biotyper platform.	beer spoilage microorganisms present a major risk for the brewing industry and can lead to cost-intensive recall of contaminated products and damage to brand reputation. the applicability of molecular profiling using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms) in combination with biotyper software was investigated for the identification of beer spoilage microorganisms from routine brewery quality control samples. reference mass spectrum profiles fo ...	2016	26857464
polyphosphate derived from lactobacillus brevis inhibits colon cancer progression through induction of cell apoptosis.	although probiotics are known to have antitumor activity, few bacteria-derived antitumor molecules have been identified. the present study explored an antitumor molecule derived from lactobacillus brevis sbl8803 (l. brevis 8803) and the mechanisms that underlie its effects. cell viability and apoptosis were assessed by a sulforhodamine b assay and terminal deoxynucleotidyl transferase dutp staining, respectively. phosphorylated extracellular signal-regulated kinase (erk) and cleaved poly adp-rib ...	2016	26851013
development of sourdough fermented date seed for improving the quality and shelf life of flat bread: study with univariate and multivariate analyses.	the aim of this work was to study the effects of a novel sourdough system prepared by wheat flour supplemented by combination of pulverized date seed, lactobacillus plantarum, and/or lactobacillus brevis as well as saccharomyces cerevisiae on the sourdough characteristics, quality, sensory, texture, shelf life and image properties of barbari flat bread. the highest sourdough acidity and bread specific volume was obtained with co-culture of lb. plantarum + lb. brevis + s. cerevisiae. the results ...	2016	26787943
efficient bioconversion of l-glutamate to γ-aminobutyric acid by lactobacillus brevis resting cells.	this work investigated the efficient bioconversion process of l-glutamate to gaba by lactobacillus brevis tccc 13007 resting cells. the optimal bioconversion system was composed of 50 g/l 48 h cultivated wet resting cells, 0.1 mm pyridoxal phosphate in glutamate-containing 0.6 m citrate buffer (ph 4.5) and performed at 45 °c and 180 rpm. by 10 h bioconversion at the ratio of 80 g/l l-glutamic acid to 240 g/l monosodium glutamate, the final titer of gaba reached 201.18 g/l at the molar bioconvers ...	2016	27155855
control of zymoseptoria tritici cause of septoria tritici blotch of wheat using antifungal lactobacillus strains.	this study explored an effective biological control agent based on lactic acid bacteria culture or culture supernatant, which was effective against fungicide-resistant zymoseptoria tritici, which causes septoria tritici blotch (stb).	2016	27155088
community dynamics and metabolite target analysis of spontaneous, backslopped barley sourdough fermentations under laboratory and bakery conditions.	barley flour is not commonly used for baking because of its negative effects on bread dough rheology and loaf volume. however, barley sourdoughs are promising ingredients to produce improved barley-based breads. spontaneous barley sourdough fermentations were performed through backslopping (every 24h, 10days) under laboratory (fermentors, controlled temperature of 30°c, high dough yield of 400) and bakery conditions (open vessels, ambient temperature of 17-22°c, low dough yield of 200), making u ...	2016	27088869
impact of lactic acid and hydrogen ion on the simultaneous fermentation of glucose and xylose by the carbon catabolite derepressed lactobacillus brevis atcc 14869.	lactobacillus brevis atcc 14869 exhibited a carbon catabolite de-repressed (ccr) phenotype which has ability to consume fermentable sugar simultaneously with glucose. to evaluate this unusual phenotype under harsh conditions during fermentation, the effect of lactic acid and hydrogen ion concentrations on l. brevis atcc 14869 were examined. kinetic equations describing the relationship between specific cell growth rate and lactic acid or hydrogen ion concentration has been reduced. the change of ...	2016	27056470
genome sequence of lactobacillus brevis strain d6, isolated from smoked fresh cheese.	the autochthonouslactobacillus brevisstrain d6, isolated from smoked fresh cheese, carries a 45-kda s-layer protein. strain d6 has shown adhesion to extracellular matrix proteins and to caco-2 intestinal epithelial cells, as well as immunomodulatory potential and beneficial milk technological properties. hence, it could be used as a potential probiotic starter culture for cheese production.	2016	27056237
lactic acid fermentation of cactus cladodes (opuntia ficus-indica l.) generates flavonoid derivatives with antioxidant and anti-inflammatory properties.	cactus pear (opuntia ficus-indica l.) is widely distributed in the arid and semi-arid regions throughout the world. in the last decades, the interest towards vegetative crop increased, and cladodes are exploited for nutraceutical and health-promoting properties. this study aimed at investigating the capacity of selected lactic acid bacteria to increase the antioxidant and anti-inflammatory properties of cactus cladodes pulp, with the perspective of producing a functional ingredient, dietary supp ...	2016	27023062
influence of gaba and gaba-producing lactobacillus brevis dpc 6108 on the development of diabetes in a streptozotocin rat model.	the aim of this study was to investigate if dietary administration of γ-aminobutyric acid (gaba)-producing lactobacillus brevis dpc 6108 and pure gaba exert protective effects against the development of diabetes in streptozotocin (stz)-induced diabetic sprague dawley rats. in a first experiment, healthy rats were divided in 3 groups (n=10/group) receiving placebo, 2.6 mg/kg body weight (bw) pure gaba or l. brevis dpc 6108 (~10(9)microorganisms). in a second experiment, rats (n=15/group) were ran ...	2016	27013462
effect of dietary heat-killed lactobacillus brevis sbc8803 (sbl88™) on sleep: a non-randomised, double blind, placebo-controlled, and crossover pilot study.	we previously reported that dietary heat-killed lactobacillus brevis sbc8803 affects sleep rhythms in mice. the present study evaluated the effect of consumption of heat-killed sbc8803 on sleep architecture in humans. a non-randomised, placebo-controlled, double blind, and crossover pilot study was conducted using volunteers who scored at a slightly high level (i.e. ≥6) on the athens insomnia scale (ais). male subjects (n=17; age 41-69 y) consumed placebo or sbc8803 capsules (25 mg/day of heat-k ...	2016	27013460
high γ-aminobutyric acid production from lactic acid bacteria: emphasis on lactobacillus brevis as a functional dairy starter.	γ-aminobutyric acid (gaba) and gaba-rich foods have shown anti-hypertensive and anti-depressant activities as the major functions in humans and animals. hence, high gaba-producing lactic acid bacteria (lab) could be used as functional starters for manufacturing novel fermented dairy foods. glutamic acid decarboxylases (gads) from lab are highly conserved at the species level based on the phylogenetic tree of gads from lab. moreover, two functionally distinct gads and one intact gad operon were o ...	2016	26980301
fermentation and microbial population dynamics during the ensiling of native grass and subsequent exposure to air.	to study the microbial population and fermentation dynamics of large needlegrass (ln) and chinese leymus (cl) during ensiling and subsequent exposure to air, silages were sampled and analyzed using culture-based techniques and denaturing gradient gel electrophoresis (dgge). a total of 112 lactic acid bacteria (lab) strains were isolated and identified using the 16s rrna sequencing method. lactic acid was not detected in the first 20 days in ln silage and the ph decreased to 6.13 after 45 days of ...	2016	26950516
lactic acid bacteria in total mixed ration silage containing soybean curd residue: their isolation, identification and ability to inhibit aerobic deterioration.	we investigated the effects of the predominant lactic acid bacteria (lab) on the fermentation characteristics and aerobic stability of total mixed ration (tmr) silage containing soybean curd residue (sc-tmr silage). the sc-tmr materials were ensiled in laboratory silos for 14 or 56 days. lab predominant in sc-tmr silage were identified (exp. 1). lactobacillus fermentum (l. fermentum) and streptococcus bovis (s. bovis) were found in the untreated materials, leuconostoc pseudomesenteroides (l. pse ...	2016	26949952
anti-inflammatory effects of lactobacillus brevis k65 on raw 264.7 cells and in mice with dextran sulphate sodium-induced ulcerative colitis.	lactic acid bacteria (lab) with anti-inflammatory effects may be beneficial to the prevention or treatment for inflammation-related diseases, such as inflammatory bowel diseases. in an in vitro assay, heat-killed lactobacillus brevis k65 (k65) reduced lipopolysaccharide-induced production of nitric oxide, tumour necrosis factor (tnf)-α and prostaglandin e2 in raw 264.7 cells. in raw 264.7 cells stably expressing an ind=ucible nitric oxide synthase (inos) reporter, viable k65 showed greater inhib ...	2016	26925602
the impact of heterologous catalase expression and superoxide dismutase overexpression on enhancing the oxidative resistance in lactobacillus casei.	two heme-dependent catalase genes were amplified from genomic dna of lactobacillus plantarum wcfs1 (kate1) and lactobacillus brevis atcc 367 (kate2), respectively, and a manganese-containing superoxide dismutase from lactobacillus casei mcjδ1 (mnsod) were cloned into plasmid pelx1, yielding pelx1-kate1, pelx1-kate2 and pelx1-mnsod, then the recombinant plasmids were transferred into l. casei mcjδ1. the strains of l. casei mcjδ1/pelx1-kate1 and l. casei mcjδ1/pelx1-kate2 were tolerant at 2 mm h2o ...	2016	26922415
biological valorization of pure and crude glycerol into 1,3-propanediol using a novel isolate lactobacillus brevis n1e9.3.3.	the aim of the study was to evaluate a novel onsite enrichment approach to isolate a crude glycerol utilizing facultative anaerobic bacteria. an onsite enrichment in natural conditions resulted an isolate, lactobacillus brevis n1e9.3.3, that can utilize glycerol and produce 1,3-propanediol with a yield of 0.89g1,3-pdo/gglycerol and productivity of 0.78g1,3-pdo/l/h at ph-8.5 under anaerobic conditions. batch fermentation experiments with glycerol-glucose co-fermentation strategy was carried out t ...	2016	26920628
biodiversity and technological-functional potential of lactic acid bacteria isolated from spontaneously fermented quinoa sourdoughs.	to analyse lactic acid bacteria (lab) diversity and technological-functional and safety properties of strains present during spontaneous fermented quinoa sourdoughs.	2016	26909667