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Functional compounds in fermented buckwheat sprouts.Fermented buckwheat sprouts (FBS) are used as multifunctional foods. Their production process includes fermentation with lactic acid bacteria. The major strains were found to include Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus pentosus, Lactococcus lactis subsp. lactis, and Pediococcus pentosaceus in an investigation of the lactic acid bacteria. We searched for the functional components, and nicotianamine (NA) and 2?-hydroxynicotianamine (HNA) were identified as angiotensin I-co ...201121897039
effect of fermented sea tangle on the alcohol dehydrogenase and acetaldehyde dehydrogenase in saccharomyces cerevisiae.sea tangle, a kind of brown seaweed, was fermented with lactobacillus brevis bj-20. the gamma-aminobutyric acid (gaba) content in fermented sea tangle (fst) was 5.56% (w/w) and gaba in total free amino acid of fst was 49.5%. the effect of fst on the enzyme activities and mrna protein expression of alcohol dehydrogenase (adh) and acetaldehyde dehydrogenase (aldh) involved in alcohol metabolism in saccharomyces cerevisiae was investigated. yeast was cultured in ypd medium supplemented with differe ...201121876367
antibiosis of vineyard ecosystem fungi against food-borne microorganisms.fermentation extracts from fungi isolated from vineyard ecosystems were tested for antimicrobial activities against a set of test microorganisms, including five food-borne pathogens (staphylococcus aureus ep167, acinetobacter baumannii (clinically isolated), pseudomonas aeruginosa pao1, escherichia coli o157:h7 (cect 5947) and candida albicans my1055) and two probiotic bacteria (lactobacillus plantarum lch17 and lactobacillus brevis lch23). a total of 182 fungi was grown in eight different media ...201122044674
culture-independent detection of microorganisms in traditional slovakian bryndza cheese.in order to investigate the microflora of slovakian bryndza cheese (a cheese containing unpasteurized or pasteurized ewes' milk component) by a culture-independent method, dna was extracted directly from 7 bryndza samples and analysed by an innovative method. using the universal prokaryotic and fungal primers, ribosomal dna internal transcribed spacer (its) regions with variable length were amplified. the standard universal reverse primer l1 aligning to bacterial 23s rdna was found unsuitable fo ...201121849217
a polysaccharide isolated from ecklonia cava fermented by lactobacillus brevis inhibits the inflammatory response by suppressing the activation of nuclear factor-κb in lipopolysaccharide-induced raw 264.7 macrophages.we previously reported that the increment of carbohydrate content in the viscozyme(®) l (novozyme corp., oklahoma city, ok, usa) extract of lactobacillus brevis-fermented ecklonia cava affected the inhibition of nitric oxide (no) production and that it might be related to the polysaccharide compound. however, there is no report of anti-inflammatory effects of the polysaccharide or its biological mechanism. here, we investigated the anti-inflammatory effects of the polysaccharide and its biologic ...201122145773
synthesis of the cancer preventive peptide lunasin by lactic acid bacteria during sourdough fermentation.this study aimed to exploit the potential of sourdough lactic acid bacteria to release lunasin during fermentation of cereal and nonconventional flours. the peptidase activities of a large number of sourdough lactic acid bacteria were screened using synthetic substrates. selected lactic acid bacteria were used as sourdough starters to ferment wholemeal wheat, soybean, barley, amaranth, and rye flours. proteinase activity during fermentation was characterized by sds-page analysis of the water-sol ...201122098174
lactobacillus brevis strain 1e1 administered to piglets through milk supplementation prior to weaning maintains intestinal integrity after the weaning event.early colonisation in the gastrointestinal tract by commensal microbes influences the progressive development and maturity of digestive and immune system functionality in the neonate. application of strategically selected direct-fed microbials to neonatal pigs may provide an opportunity to dictate a portion of the intestinal microbial community and exert a beneficial influence on these developmental processes. experiments were conducted to determine the effects of early administration of lactoba ...201121831788
Lactobacillus brevis responds to flavonoids through KaeR, a LysR-type of transcriptional regulator.The ability of transcription factors to respond to flavonoids as signal molecules was investigated in Lactobacillus brevis. Through in vitro screening of a small library of flavonoids, LVIS1989 (KaeR), a LysR-type transcriptional regulator (LTTR), was identified as responsive to kaempferol. The modulation of KaeR activity by flavonoids was characterized in vivo and in vitro. DNase I footprint assays identified the binding of KaeR at two distinctive sites, one in the intergenic region between LVI ...201121819457
antioxidant effects of fermented sea tangle (laminaria japonica) by latobacillus brevis bj20 in individuals with high level of γ-gt: a randomized, double-blind, and placebo-controlled clinical study.a randomized, double-blind, and placebo-controlled clinical study was performed to evaluate the antioxidant effects of fermented sea tangle (fst) on healthy volunteers with high levels of γ-glutamyltransferse (γ-gt). forty-eight participants were divided into a placebo group and an fst group that received fst (1.5g/day) for 4weeks. serum γ-gt, malondialdehyde (mda), catalase (cat), superoxide dismutase (sod), and glutathione peroxidase (gpx) activities were determined before and after the trial. ...201122138360
Isolation of a Virulent Lactobacillus brevis Phage and Its Application in the Control of Beer Spoilage.Beer quality can be compromised by the growth of certain lactobacilli, in particular Lactobacillus brevis and Lactobacillus plantarum, and various strategies have been used to control such bacterial spoilage. Biocontrol by means of bacteriophage is a reemerging approach for the suppression of spoilage bacteria in food and beverage matrices. A virulent phage capable of infecting L. brevis beer-spoilage strains was isolated and morphologically assessed by electron microscopy. The myophage SA-C12 ...201122186058
genotypic and phenotypic characterization of lactic acid bacteria isolated from italian ryegrass silage.twenty-three lactic acid bacteria (lab) isolated from three cultivars (akiaoba, nagahahikari and tachiwase) of italian ryegrass (lolium multiflorum lam.) silage were precisely characterized by a combination of phenotypic tests, genotypic 16s ribosomal dna sequencing and rapid pcr-based analyses, focusing on their useful phenotypes for silage preparation as inoculants. we successfully identified both at the species and subspecies levels: phenotypically novel lactococcus lactis subsp. lactis, lact ...201122339691
characterization of lactic acid bacteria from musts and wines of three consecutive vintages of ribeira sacra.this study was designed to isolate and characterize the lactic acid microbiota of the musts and wines of a young denomination of origin area, ribeira sacra in north-west spain.201121204877
phenotypic and genotypic analyses of lactic acid bacteria in local fermented food, breast milk and faeces of mothers and their babies.lactic acid bacteria (lab) are generally accepted as beneficial to the host and their presence is directly influenced by ingestion of fermented food or probiotics. while the intestinal lactic microbiota is well-described knowledge on its routes of inoculation and competitiveness towards selective pressure shaping the intestinal microbiota is limited. in this study, lab were isolated from faecal samples of breast feeding mothers living in syria, from faeces of their infants, from breast milk as w ...201121300508
glycemic index and phenolics of partially-baked frozen bread with sourdough.different lactic acid bacteria starters were used to prepare sourdough to make partially-baked frozen wholemeal wheat bread. the sourdough was prepared with a pure culture of lactobacillus plantarum or with commercial starters containing lactobacillus brevis combined with saccharomyces cerevisiae var. chevalieri (lv4), lactobacillus fermentum (pl1), or lactobacillus fermentum with phytase (pl3). we determined the acetic and lactic acid concentrations in sourdough, bread chemical composition, tot ...201120715901
synthesis of +¦-aminobutyric acid by expressing lactobacillus brevis-derived glutamate decarboxylase in the corynebacterium glutamicum strain atcc 13032.purpose of work: purpose of this work is to synthesize +¦-aminobutyric acid by glutamate-producing species expressing lactobacillus brevis-derived glutamate decarboxylase genes, i.e. recombinant corynebacterium glutamicum strains, which directly convert endogenous l: -glutamate precursor into +¦-aminobutyric acid (gaba) through single-step fermentation. to express exogenous glutamate decarboxylase (gad) in an l: -glutamate-producing strain, lactobacillus brevis lb85, which can produce gaba, was ...201121826397
bacterial and fungal communities of wilted italian ryegrass silage inoculated with and without lactobacillus rhamnosus or lactobacillus buchneri.to understand the effects of lactic acid bacteria (lab) inoculation on fermentation products, aerobic stability and microbial communities of silage.201121204884
heat-killed body of lactobacillus brevis sbc8803 ameliorates intestinal injury in a murine model of colitis by enhancing the intestinal barrier function.probiotics have been clinically administered to improve intestinal damage in some intestinal inflammations. however, probiotic treatments are not always effective for these intestinal disorders because live bacteria must colonize and maintain their activity under unfavorable conditions in the intestinal lumen when displaying their functions. this study investigated the physiological functions of a heat-killed body of a novel probiotic, lactobacillus brevis sbc8803, on the protection of intestina ...201121987297
expression of lactobacillus brevis ioeb 9809 tyrosine decarboxylase and agmatine deiminase genes in wine correlates with substrate availability.aims:ôçé lactobacillus brevis ioeb 9809 is able to produce both tyramine and putrescine via tyrosine decarboxylase and agmatine deiminase enzymes, respectively, when cultured on synthetic media. the aims of this study were to assess the expression of l.-ábrevis ioeb 9809 tdc and agua1 genes, during wine fermentation and to evaluate the effect of substrate availability and ph on tdc and agua1 expression, as well as on biogenic amine production and l.-ábrevis viability. methods and results:ôçé the ...201121740449
Combination of culture-dependent and culture-independent molecular methods for the determination of lactic microbiota in sucuk.In this study, the culture-dependent and culture-independent molecular methods were used for the identification of lactic acid bacteria (LAB) in sucuk a Turkish fermented dry sausage. On the one hand, the PCR-DGGE method targetting the V1 and V3 regions of 16S DNA was applied to DNA that was directly extracted from sucuk samples. On the other hand, rep-PCR fingerprinting was performed for the primary differentiation and grouping of the isolates, and the results were confirmed by sequencing of th ...201122209604
Probiotic-derived polyphosphate enhances the epithelial barrier function and maintains intestinal homeostasis through integrin-p38 MAPK pathway.Probiotics exhibit beneficial effects on human health, particularly in the maintenance of intestinal homeostasis in a complex manner notwithstanding the diversity of an intestinal flora between individuals. Thus, it is highly probable that some common molecules secreted by probiotic and/or commensal bacteria contribute to the maintenance of intestinal homeostasis and protect the intestinal epithelium from injurious stimuli. To address this question, we aimed to isolate the cytoprotective compoun ...201121858054
fermentation quality and chemical composition of shrub silage treated with lactic acid bacteria inoculants and cellulase additives.effects of lactic acid bacteria (lab) inoculants and cellulase additives on fermentation quality and chemical compositions of shrub silages were studied by using a small-scale fermentation system. two lab inoculants of qingbao (lactobacillus plantarum, pediococcus acidilacticii, lactobacillus casei and clostridium phage) and caihe (lactobacillus plantarum, lactobacillus brevis and pediococcus acidilactici) and a commercial cellulase made from trichoderma reesei were used as additives for interme ...201122515690
phylogenetic analysis of a spontaneous cocoa bean fermentation metagenome reveals new insights into its bacterial and fungal community diversity.this is the first report on the phylogenetic analysis of the community diversity of a single spontaneous cocoa bean box fermentation sample through a metagenomic approach involving 454 pyrosequencing. several sequence-based and composition-based taxonomic profiling tools were used and evaluated to avoid software-dependent results and their outcome was validated by comparison with previously obtained culture-dependent and culture-independent data. overall, this approach revealed a wider bacterial ...201222666442
reductive whole-cell biotransformation with corynebacterium glutamicum: improvement of nadph generation from glucose by a cyclized pentose phosphate pathway using pfka and gapa deletion mutants.in this study, the potential of corynebacterium glutamicum for reductive whole-cell biotransformation is shown. the nadph-dependent reduction of the prochiral methyl acetoacetate (maa) to the chiral (r)-methyl 3-hydroxybutyrate (mhb) by an alcohol dehydrogenase from lactobacillus brevis (lbadh) was used as model reaction and glucose served as substrate for the regeneration of nadph. since nadph is mainly formed in the oxidative branch of the pentose phosphate pathway (ppp), c. glutamicum was eng ...201222851018
γ-aminobutyric acid production by culturable bacteria from the human intestine.to assess the ability of human intestinally derived strains of lactobacillus and bifidobacterium to produce γ-aminobutyric acid (gaba).201222612585
effects of bile salt deconjugation by probiotic strains on the survival of antibiotic-resistant foodborne pathogens under simulated gastric conditions.this study was designed to evaluate the effects of bile acid deconjugation by probiotic strains on the antibiotic susceptibility of antibiotic-sensitive and multiple antibiotic-resistant salmonella typhimurium and staphylococcus aureus. eight probiotic strains, bifidobacterium longum b6, lactobacillus acidophilus adh, lactobacillus brevis kacc 10553, lactobacillus casei kacc 12413, lactobacillus paracasei atcc 25598, lactobacillus rhamnosus gg, leuconostoc mesenteroides kacc 12312, and pediococc ...201222691477
gaseous co2 signal initiates growth of butyric-acid-producing clostridium butyricum in both pure culture and mixed cultures with lactobacillus brevis.microbial strains produce numerous volatile substances in the anaerobic conditions of the human intestines. the availability of co(2) is known to be a prerequisite for bacterial growth in general. in experiments with anaerobic lactobacillus brevis and clostridium butyricum bacteria in the portable microbial enrichment unit (pmeu) it was shown that these strains interact; this interaction being mediated by co(2) emission. co(2) promoted clostridial growth in pure cultures and mixed cultures with ...201222697044
production optimization of invertase by lactobacillus brevis mm-6 and its immobilization on alginate beads.a sequential optimization strategy, based on statistical experimental designs, was employed to enhance the production of invertase by lactobacillus brevis mm-6 isolated from breast milk. first, a 2-level plackett-burman design was applied to screen the bioprocess parameters that significantly influence the invertase production. the second optimization step was performed using fractional factorial design in order to optimize the amounts of variables have the highest positive significant effect on ...201223499118
production of aromatic compounds by metabolically engineered escherichia coli with an expanded shikimate pathway.escherichia coli was metabolically engineered by expanding the shikimate pathway to generate strains capable of producing six kinds of aromatic compounds, phenyllactic acid, 4-hydroxyphenyllactic acid, phenylacetic acid, 4-hydroxyphenylacetic acid, 2-phenylethanol, and 2-(4-hydroxyphenyl)ethanol, which are used in several fields of industries including pharmaceutical, agrochemical, antibiotic, flavor industries, etc. to generate strains that produce phenyllactic acid and 4-hydroxyphenyllactic ac ...201222752168
the effect of a silage inoculant on silage quality, aerobic stability, and meat production on farm scale.the effect of inoculation on nutrient content, fermentation, aerobic stability, and beef cattle performance for whole-plant corn silage treated with a commercial product (blend of homo- and heterofermentative lactic acid bacteria, bsm, blend of enterococcus faecium, lactobacillus plantarum, and lactobacillus brevis, dsm numbers 3530, 19457, and 23231, resp.), was compared to a control treatment with no silage additives (ct). the material had a dm of 323 g/kg, crude protein, and water-soluble car ...201223738122
antimicrobial susceptibility of microflora from ovine cheese.strains identified in ovine cheese and bryndza by matrix-assisted laser desorption/ionization time-of-flight analysis belonged to ten species of non-enterococcal lactic acid bacteria and included lactobacillus casei/lactobacillus paracasei, lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus helveticus, lactobacillus delbrueckii, lactobacillus fermentum, lactobacillus brevis, lactococcus lactis, pediococcus pentosaceus and pediococcus acidilactici. the susceptibility toward antibioti ...201222491987
fructooligosaccharides metabolism and effect on bacteriocin production in lactobacillus strains isolated from ensiled corn and molasses.fructo- (fos) and galacto-oligosaccharides have been used to promote the growth of probiotics, mainly those from lactobacillus genus. however, only few reports have evaluated the effect of prebiotics on bacteriocins activity and production. in this work, we characterized the effect of fos supplementation on the growth, lactic and acetic acids production, and antimicrobial activity of crude extracts obtained from lactobacillus strains isolated from ensiled corn and molasses. seven out of 28 isola ...201222342961
basic characterization and partial purification of β-glucosidase from cell-free extracts of oenococcus oeni st81.this study was designed to characterize a β-glucosidase of oenococcus oeni st81, a strain isolated from a spanish wine of the origin appellation ribeira sacra.201222748149
survival of silage lactic acid bacteria in the goat gastrointestinal tract as determined by denaturing gradient gel electrophoresis.to determine the survival rate of silage lactic acid bacteria (lab) in the ruminant gastrointestinal tract.201222925065
the two-step biotransformation of monosodium glutamate to gaba by lactobacillus brevis growing and resting cells.gama-aminobutyric acid (gaba) is a natural functional amino acid. in the current study, lactobacillus brevis tccc13007, a high gaba-producing strain, was isolated from naturally pickled chinese vegetables. a two-step cellular bioconversion process was established using l. brevis tccc13007 for the production of gaba. first, l. brevis cells were grown anaerobically in 7% monosodium glutamate (msg)-containing medium at an initial ph of 6.0 and a controlled ph of 4.6 for 16 to 66 h; approximately 38 ...201222307498
insights into the completely annotated genome of lactobacillus buchneri cd034, a strain isolated from stable grass silage.lactobacillus buchneri belongs to the group of heterofermentative lactic acid bacteria and is a common member of the silage microbiome. here we report the completely annotated genomic sequence of l. buchneri cd034, a strain isolated from stable grass silage. the whole genome of l. buchneri cd034 was sequenced on the roche genome sequencer flx platform. it was found to consist of four replicons, a circular chromosome, and three plasmids. the circular chromosome was predicted to encode 2319 protei ...201222465289
c-terminal truncation of glutamate decarboxylase from lactobacillus brevis cgmcc 1306 extends its activity toward near-neutral ph.glutamate decarboxylase (gad) from lactobacillus brevis is a very promising candidate for biosynthesis of gaba and various other bulk chemicals that can be derived from gaba. however, no structure of gad of this origin has been reported to date, which limits enzyme engineering strategy to improve its properties for better use in production of gaba. bacterial gad exhibits an acidic ph optimum and there is often a sharp ph dependence. in the present work, site-directed mutagenesis was performed to ...201222418267
tight controlled expression and secretion of lactobacillus brevis slpa in lactococcus lactis.prokaryotes commonly present outer cell wall structures composed of a crystalline array of proteinaceous subunits, known as surface layers (s-layers). the orf encoding the s-layer protein (slpa) of lactobacillus brevis was cloned into lactococcus lactis under the transcriptional control of the xylose-inducible expression system (xies). slpa was secreted into the extracellular medium, as determined by immunoblotting, and assays on the kinetics of slpa production revealed that repression of the sy ...201222391736
aphthous oral ulceration and its successful management by lactobacillus brevis cd2 extract in an adult haemophilic patient. 201222335552
prevalent lactic acid bacteria in cider cellars and efficiency of oenococcus oeni strains.malolactic fermentation (mlf) is an important step in cider production in order to allowing for improvement of microbiological stability and organoleptic characteristics of cider. induction of this fermentation by using starter cultures enables a better control over this bioprocess, but although it is a common practice in winemaking, starters specifically focussed for cider mlf are not yet commercially available. proper starter cultures need to present the ability to degrade l-malic acid conferr ...201222850371
lactic acid bacteria isolated from apples are able to catabolise arginine.we investigated the potentiality of lactic acid bacteria (lab) isolated from two apples variety to utilize arginine at different initial ph values. apples surface contained average levels of bacteria ranging from log 2.49 ± 0.53 to log 3.73 ± 0.48 cfu/ml for red delicious and golden delicious varieties, respectively. thirty-one strains able to develop in presence of arginine at low ph were phenotypically and genotipically identified as belonging to lactobacillus, pediococcus and leuconostoc gene ...201222805821
fructophilic lactobacillus kunkeei and lactobacillus brevis isolated from fresh flowers, bees and bee-hives.two-hundred-and-thirty-six isolates were collected from fresh flowers, bees and bee-hives. of these, 20 isolates preferred d-fructose as carbon source, produced lactic acid and acetic acid but trace amounts of ethanol and were classified as fructophilic. poor growth was recorded when strains were incubated anaerobically in the presence of d-glucose as sole carbon source. good growth was, however, recorded when d-glucose was metabolized in the presence of external electron acceptors such as fruct ...201222797888
enhanced acetoin production by serratia marcescens h32 with expression of a water-forming nadh oxidase.cofactor engineering was employed to enhance production of acetoin by serratia marcescens h32. 2,3-butanediol was a major byproduct of acetoin fermentation by s. marcescens h32. in order to decrease 2,3-butanediol formation and achieve a high efficiency of acetoin production, nox gene encoding a water-forming nadh oxidase from lactobacillus brevis was expressed. batch fermentations suggested the expression of the nadh oxidase could increase the intracellular nad(+) concentration (1.5-fold) and n ...201222728188
evaluation of lactic acid bacterium from chilli waste as a potential antifungal agent for wood products.the aim of this study was to isolate lactic acid bacteria from chilli waste and evaluate metabolites produced for the ability to arrest wood decay.201222321006
the role of nisin in fuel ethanol production with saccharomyces cerevisiae.to investigate the effects of nisin on lactobacilli contamination of yeast during ethanol fermentation and to determine the appropriate concentration required to control the growth of selected lactobacilli in a yp/glucose media fermentation model.201222691226
investigation of asymmetric alcohol dehydrogenase (adh) reduction of acetophenone derivatives: effect of charge density.in an effort to study the effect of substituent groups of the substrate on the alcohol dehydrogenase (adh) reductions of aryl-alkyl ketones, several derivatives of acetophenone have been evaluated against adhs from lactobacillus brevis (lb) and thermoanaerobacter sp. (t). interestingly, ketones with non-demanding (neutral) para-substituents were reduced to secondary alcohols by these enzymes in enantiomerically pure form whereas those with demanding (ionizable) substituents could not be reduced. ...201222609978
biochemical, microbiological and phytochemical studies on aqueous-fermented extracts from atropa belladonna l. part 1--biochemistry and microbiology.extraction methods of fresh plants into aqueous-fermented extracts according to german homoeopathic pharmacopoeia (hab), regulation nos. 33 and 34 were evaluated. in the course of production, the extraction is accompanied by fermentation and the resulting preparation is stored for at least 6 months until further processing. the present work aimed at revealing the underlying biochemical reactions during manufacture and storage. in addition, the responsible microorganisms were isolated and identif ...201222570940
rt-qpcr analysis of putative beer-spoilage gene expression during growth of lactobacillus brevis bso 464 and pediococcus claussenii atcc baa-344(t) in beer.lactic acid bacteria (lab) contamination of beer presents a continual economic threat to brewers. interestingly, only certain isolates of lab can grow in the hostile beer environment (e.g., as studied here, lactobacillus brevis bso 464 (lb464) and a non-ropy isolate of pediococcus claussenii atcc baa-344(t) (pc344nr)), indicating that significant genetic specialization is required. the genes hita, hora, horb, horc, and bsra, which have been proposed to confer beer-spoiling ability to an organism ...201222893225
the effect of lactobacillus brevis kb290 against irritable bowel syndrome: a placebo-controlled double-blind crossover trial.irritable bowel syndrome (ibs) is a functional disorder of the digestive tract that causes chronic abdominal symptoms. we evaluated the effects of lactobacillus brevis kb290 (kb290), which has been demonstrated to be effective at improving bowel movements and the composition of intestinal microflora, on ibs symptoms.201222863114
bacteriophage ecology in a commercial cucumber fermentation.to reduce high-salt waste from cucumber fermentations, low-salt fermentations are under development. these fermentations may require the use of starter cultures to ensure normal fermentations. because potential phage infection can cause starter culture failure, it is important to understand phage ecology in the fermentations. this study investigated the phage ecology in a commercial cucumber fermentation. brine samples taken from a fermentation tank over a 90-day period were plated onto deman-ro ...201223023756
protective effect of lactofermented red beetroot juice against aberrant crypt foci formation, genotoxicity of fecal water and oxidative stress induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine in rats model.the aim of the study was to investigate the effects of beetroot juice fermented by lactobacillus brevis 0944 and lactobacillus paracasei 0920 (fbj) on carcinogen induction of aberrant crypt foci (acf) in rat colon. 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (phip) was used as carcinogen, which was administrated intragastrically at a dose of 10 μg/day, every day of the experiment. additionally, we investigated the cytotoxicity and genotoxicity of fecal water from experimental animals in the ...201222995401
tyrosine-containing peptides are precursors of tyramine produced by lactobacillus plantarum strain ir bl0076 isolated from wine.biogenic amines are molecules with allergenic properties. they are found in fermented products and are synthesized by lactic acid bacteria through the decarboxylation of amino acids present in the food matrix. the concentration of biogenic amines in fermented foodstuffs is influenced by many environmental factors, and in particular, biogenic amine accumulation depends on the quantity of available precursors. enological practices which lead to an enrichment in nitrogen compounds therefore favor b ...201222963406
characterization of lactose utilization and β-galactosidase in lactobacillus brevis kb290, the hetero-fermentative lactic acid bacterium.unlike dairy lactic acid bacteria, lactobacillus brevis cannot ferment milk. we characterized the lactose utilization by l. brevis kb290. in a carbohydrate fermentation assay using api 50 chl, we showed during 7 days l. brevis did not ferment lactose. l. brevis grew to the stationary phase in 2 weeks in mrs broth containing lactose as the carbon source. l. brevis slowly consumed the lactose in the medium. l. brevis hydrolyzed lactose and a lactose analog, o-nitrophenyl-β-d-galactopyranoside (onp ...201222936499
characterization of lactic acid bacteria isolated from sauce-type kimchi.this study was carried out to investigate the isolation and characterization of lactic acid bacteria (lab) from naturally fermented sauce-type kimchi. sauce-type kimchi was prepared with fresh, chopped ingredients (korean cabbage, radish, garlic, ginger, green onion, and red pepper). the two isolated bacteria from sauce-type kimchi were identified as pediococcus pentosaceus and lactobacillus brevis by 16s rdna sequencing and tentatively named pediococcus sp. ij-k1 and lactobacillus sp. ij-k2, re ...201224471087
phenotypic and phylogenetic analysis of lactic acid bacteria isolated from forage crops and grasses in the tibetan plateau.a total of 140 lactic acid bacteria (lab) strains were isolated from corn, alfalfa, clover, sainfoin, and indian goosegrass in the tibetan plateau. according to phenotypic and chemotaxonomic characteristics, 16s rdna sequence, and reca gene pcr amplification, these lab isolates were identified as belonging to five genera and nine species. corn contained more lab species than other forage crops. leuconostoc pseudomesenteroides, lactococcus lactis subsp. lactis, lactobacillus brevis, and weissella ...201222367939
fermenting red ginseng enhances its safety and efficacy as a novel skin care anti-aging ingredient: in vitro and animal study.the objective of this study was to evaluate the anti-aging potential and skin safety of red ginseng (rg) and fermented red ginseng (frg) using lactobacillus brevis for use as cosmetic ingredients. concentrations of uronic acid, polyphenols, and flavonoids, and antioxidant activities were greater in frg compared to rg. the contents of total ginsenosides were not significantly different. however, the ginsenoside metabolite content was higher in frg (14,914.3 μg/ml) compared to rg (5697.9 μg/ml). t ...201223126662
biogenic amine production by the wine lactobacillus brevis ioeb 9809 in systems that partially mimic the gastrointestinal tract stress.ingestion of fermented foods containing high levels of biogenic amines (ba) can be deleterious to human health. less obvious is the threat posed by ba producing organisms contained within the food which, in principle, could form ba after ingestion even if the food product itself does not initially contain high ba levels. in this work we have investigated the production of tyramine and putrescine by lactobacillus brevis ioeb 9809, of wine origin, under simulated gastrointestinal tract (git) condi ...201223113922
arabinoxylan oligosaccharide hydrolysis by family 43 and 51 glycosidases from lactobacillus brevis dsm 20054.due to their potential prebiotic properties, arabinoxylan-derived oligosaccharides [(a)xos] are of great interest as functional food and feed ingredients. while the (a)xos metabolism of bifidobacteriaceae has been extensively studied, information regarding lactic acid bacteria (lab) is still limited in this context. the aim of the present study was to fill this important gap by characterizing candidate (a)xos hydrolyzing glycoside hydrolases (ghs) identified in the genome of lactobacillus brevis ...201323995921
effect of orally administered lactobacillus brevis hy7401 in a food allergy mouse model.we had found that orally administered lactobacillus species were effective immune modulators in ovalbumin (ova)-sensitized mice. to validate these findings, we investigated the effects of orally administered lactobacillus brevis hy7401 in ova-t cell receptor transgenic mice. this strain showed a tendency to induce th1 cytokines and inhibit th2 cytokines. all assayed isotypes of ova-specific antibody were effectively reduced. systemic anaphylaxis was also relatively reduced with the probiotic adm ...201323985541
lactobacillus yonginensis sp. nov., a lactic acid bacterium with ginsenoside converting activity isolated from kimchi.a gram-reaction-positive, non-motile, non-spore-forming, catalase-negative, facultatively anaerobic, rod-shaped, β-glucosidase-producing lactic acid bacterium, designated strain thk-v8(t), was isolated from the korean fermented food, kimchi, and its taxonomic position was investigated by using a polyphasic approach. strain thk-v8(t) was able to grow at 4-40 °c (optimum, 30 °c) and ph 4.0-7.0 (optimum, ph 6.0). strain thk-v8(t) had the ability to transform ginsenoside rb₁ to rd. on the basis of 1 ...201323475345
purification and characterization of β-d-xylosidase from lactobacillus brevis grown on xylo-oligosaccharides.in the recent years there has been a growing interest in the use of oligosaccharides as prebiotics to modulate gut microbiota with an aim to improve the gut health. though xylo-oligosaccharides (xos) have been increasingly used as prebiotics, information pertaining to the enzymes used by lactobacilli to degrade these substrates is scanty. present investigation reports the purification and characterization of β-d-xylosidase from lactobacillus brevis ncdc01 grown on xos. three sequential steps con ...201323399247
effects of intraduodenal injection of lactobacillus brevis sbc8803 on autonomic neurotransmission and appetite in rodents.lactobacilli provide several health benefits to mammals, including humans. we previously observed that in rats, intraduodenal injection of lactobacillus johnsonii la1 elevated efferent gastric vagal nerve activity (efferent-gvna), while lactobacillus paracasei st11 suppressed efferent-gvna, and thereby increased or decreased food intake. to determine the function of lactobacillus brevis (sbc8803), its effect on food intake was examined by providing food containing heat-killed sbc8803 to mice. we ...201323391754
lactobacillus brevis kb290 enhances il-8 secretion by vibrio parahaemolyticus-infected caco-2 cells.vibrio parahaemolyticus in uncooked seafood causes acute gastroenteritis. the microorganism has two sets of type iii secretion systems and two hemolysins. when it injects its effector proteins into a host cell via type iii secretion system 1, one of the type iii secretion systems induces secretion of interleukin (il)-8, a proinflammatory chemokine, through the phosphorylation of erk 1/2 and p38 mapk. although probiotics have beneficial effects on hosts and can help control some infectious diseas ...201323314377
effect of ethanol and low ph on citrulline and ornithine excretion and arc gene expression by strains of lactobacillus brevis and pediococcus pentosaceus.the accumulation of citrulline and ornithine in wine or beer as a result of the arginine catabolism of some lactic acid bacteria (lab) species increases the risk of ethyl carbamate and putrescine formation, respectively. several lab species, which are found as spoilage bacteria in alcoholic beverages, have been reported to be arginine degrading. this study evaluates the effect of ethanol content and low ph on the excretion of citrulline and ornithine by two strains belonging to the potential con ...201323122508
crystal structure of a folate energy-coupling factor transporter from lactobacillus brevis.atp-binding cassette (abc) transporters, composed of importers and exporters, form one of the biggest protein superfamilies that transport a variety of substrates across the membrane, powered by atp hydrolysis. most abc transporters are composed of two transmembrane domains and two cytoplasmic nucleotide-binding domains. also, importers from prokaryotes usually have extra solute-binding proteins in the periplasm that are responsible for the binding of substrates. structures of importers have bee ...201323584589
structure of a bacterial energy-coupling factor transporter.the energy-coupling factor (ecf) transporters constitute a novel family of conserved membrane transporters in prokaryotes that have a similar domain organization to the atp-binding cassette transporters. each ecf transporter comprises a pair of cytosolic atpases (the a and a' components, or ecfa and ecfa'), a membrane-embedded substrate-binding protein (the s component, or ecfs) and a transmembrane energy-coupling component (the t component, or ecft) that links the ecfa-ecfa' subcomplex to ecfs. ...201323584587
fast identification of wine related lactic acid bacteria by multiplex pcr.the microflora of must and wine consists of yeasts, acetic acid bacteria and lactic acid bacteria (lab). the latter group plays an important role for wine quality. the malolactic fermentation carried out by lab leads to deacidification and stabilisation of wines. nevertheless, lab are often associated with wine spoilage. they are mainly responsible for the formation of biogenic amines. furthermore, some strains produce exopolysaccharide slimes, acetic acid, diacetyl and other off-flavours. in th ...201323122500
gada gene locus in lactobacillus brevis ncl912 and its expression during fed-batch fermentation.normally, lactobacillus brevis has two glutamate decarboxylase (gad) genes; gada and gadb. using pcr, we cloned the gada gene from l. brevis strain ncl912, a high yield strain for the production of gamma-aminobutyric acid (gaba). however, despite using 61 different primer pairs, including degenerate primers from conserved regions, we were unable to use pcr to clone gadb from the ncl912 strain. furthermore, we could not clone it by genomic walking over 3000 bp downstream of the aldo-keto reductas ...201324164637
co(ii) and cd(ii) complexes derived from heterocyclic schiff-bases: synthesis, structural characterisation, and biological activity.new monomeric cobalt and cadmium complexes with schiff-bases, namely, n'-[(e)-(3-hydroxy-4-methoxyphenyl)methylidene]furan-2-carbohydrazide (l¹) and n'-[(e)-(3-hydroxy-4-methoxyphenyl)methylidene]thiophene-2-carbohydrazide (l²) are reported. schiff-base ligands l¹ and l² were derived from condensation of 3-hydroxy-4-methoxybenzaldehyde (iso-vanillin) with furan-2-carboxylic acid hydrazide and thiophene-2-carboxylic acid hydrazide, respectively. complexes of the general formula [m(l)₂]cl₂ (where ...201324027449
metagenome analyses reveal the influence of the inoculant lactobacillus buchneri cd034 on the microbial community involved in grass ensiling.silage is green fodder conserved by lactic acid fermentation performed by epiphytic lactic acid bacteria under anaerobic conditions. to improve the ensiling process and the quality of the resulting silage, starter cultures are added to the fresh forage. a detailed analysis of the microbial community playing a role in grass ensiling has been carried out by high throughput sequencing technologies. moreover, the influence of the inoculant lactobacillus buchneri cd034 on the microbial community comp ...201323880441
a review on traditional turkish fermented non-alcoholic beverages: microbiota, fermentation process and quality characteristics.shalgam juice, hardaliye, boza, ayran (yoghurt drink) and kefir are the most known traditional turkish fermented non-alcoholic beverages. the first three are obtained from vegetables, fruits and cereals, and the last two ones are made of milk. shalgam juice, hardaliye and ayran are produced by lactic acid fermentation. their microbiota is mainly composed of lactic acid bacteria (lab). lactobacillus plantarum, lactobacillus brevis and lactobacillus paracasei subsp. paracasei in shalgam fermentati ...201323859403
bioconversion of flavones during fermentation in milk containing scutellaria baicalensis extract by lactobacillus brevis.scutellaria baicalensis (sb), a traditional herb with high pharmacological value, contains more than 10% flavone by weight. to improve the biological activity of flavones in sb, we aimed to enhance the bioconversion of baicalin (bg) to baicalein (b) and wogonoside (wg) to wogonin (w) in sb during fermentation using beta-glucuronidase produced from lactobacillus brevis ro1. after activation, l. brevis ro1 was cultured in milk containing sb root extract with various carbon or nitrogen sources at 3 ...201323851266
dynamics and rrna transcriptional activity of lactococci and lactobacilli during cheddar cheese ripening.cheddar cheese is a complex ecosystem where both the bacterial population and the cheese making process contribute to flavor and texture development. the aim of this study was to use molecular methods to evaluate the impact of milk heat treatment and ripening temperature on starter lactococci and non-starter lactic acid bacteria (nslab) throughout ripening of cheddar cheese. eight cheddar cheese batches were manufactured (four with thermized and four with pasteurized milk) and ripened at 4, 7 an ...201323850855
species diversity, community dynamics, and metabolite kinetics of spontaneous leek fermentations.leek (allium ampeloprasum var. porrum) is one of belgium's most important vegetables. all or part of the green leek parts are often left on the fields because of their limited cooking applications compared to the white leek parts. therefore, the possibility to perform leek fermentations in view of product valorization and diversification was investigated. this study deals with the community dynamics, species diversity, and metabolite kinetics of spontaneous leek fermentations, thereby studying t ...201323200651
selenium nanoparticle-enriched lactobacillus brevis causes more efficient immune responses in vivo and reduces the liver metastasis in metastatic form of mouse breast cancer.selenium enriched lactobacillus has been reported as an immunostimulatory agent which can be used to increase the life span of cancer bearing animals. lactic acid bacteria can reduce selenium ions to elemental selenium nanoparticles (senps) and deposit them in intracellular spaces. in this strategy two known immunostimulators, lactic acid bacteria (lab) and senps, are concomitantly administered for enhancing of immune responses in cancer bearing mice.201323631392
effects of intake of pickles containing lactobacillus brevis on immune activity and bowel symptoms in female students.forty-four female students with a tendency for constipation (mean age, 20.2±3.3 y) were asked to consume 30 g test pickles daily for 2 wk and were divided into 3 groups: viable-cell intake subjects (n=14, 3.0×10⁵ colony-forming units of viable lab (lactic acid bacteria) cells per sample), dead-cells intake subjects (n=15, viable cells were heat sterilized), and placebo-intake subjects (n=15, lab removed from the pickles). γ-aminobutyric acid content of 75.1±3.2 mg per sample was noted, with no m ...201324418874
draft genome sequence of lactobacillus brevis strain ew, a drosophila gut pathobiont.lactobacillus brevis strain ew, a gut pathobiont, was isolated from the fruit fly drosophila melanogaster. here, we report the draft genome sequence of l. brevis ew.201324265492
enhancement of γ-aminobutyric acid production in recombinant corynebacterium glutamicum by co-expressing two glutamate decarboxylase genes from lactobacillus brevis.γ-aminobutyric acid (gaba), a non-protein amino acid, is a bioactive component in the food, feed and pharmaceutical fields. to establish an effective single-step production system for gaba, a recombinant corynebacterium glutamicum strain co-expressing two glutamate decarboxylase (gad) genes (gadb1 and gadb2) derived from lactobacillus brevis lb85 was constructed. compared with the gaba production of the gadb1 or gadb2 single-expressing strains, gaba production by the gadb1-gadb2 co-expressing st ...201323928903
cell-bound exopolysaccharides of lactobacillus brevis kb290: protective role and monosaccharide composition.we examined the survivability of lactobacillus brevis kb290 and derivative strain kb392 in artificial digestive juices and bile salts. the strains have similar membrane fatty acids but different amounts of cell-bound exopolysaccharides (eps). in artificial digestive juices, kb290 showed significantly higher survivability than kb392, and homogenization, which reduced the amount of eps in kb290 but not in kb392, reduced the survivability only of kb290. in bile salts, kb290 showed significantly hig ...201323898998
biowaste: a lactobacillus habitat and lactic acid fermentation substrate.composite organic waste was assessed for its physical, chemical and microbial suitability to serve as a substrate for the fermentative production of lactic acid. the biowaste studied was highly acidic (ph 4.3) and had high organic carbon content (45%). a clone library identified 90% of the bacterial community were lactic acid bacteria, mainly represented by lactobacilli (70%). cultivation using semiselective media identified lactobacillus plantarum, lactobacillus brevis and their closest relativ ...201323816359
lactobacillus brevis g-101 ameliorates colitis in mice by inhibiting nf-κb, mapk and akt pathways and by polarizing m1 macrophages to m2-like macrophages.we isolated lactobacillus brevis g-101 from kimchi lactic acid bacteria (lab) strains, which induced il-10 expression in lipopolysaccharide (lps)-stimulated peritoneal macrophages. to evaluate the inflammatory effect of g-101, we examined its inhibitory effect in 2,4,6-trinitrobenzene sulfonic acid (tnbs)-induced colitic mice.201323742179
putrescine production from different amino acid precursors by lactic acid bacteria from wine and cider.the aim of this work was to study the production of biogenic amines and particularly putrescine in lactic acid bacteria (lab) related to wine and cider. we applied an analytical protocol that involves the use of pcr and tlc techniques to determine the production of putrescine from different precursors. moreover, we also studied the ability of the lactobacillus and pediococcus tested to produce histamine and tyramine. the results showed that the majority of the lactobacillus brevis analyzed harbo ...201323685467
improvement of mannitol production by lactobacillus brevis mutant 3-a5 based on dual-stage ph control and fed-batch fermentations.lactobacillus brevis 3-a5 was isolated and expected to produce mannitol efficiently by regulating ph in batch and fed-batch fermentations. in 48 h batch fermentations with free and constant ph, the optimal ph for cell growth and mannitol production in the first 24 h of incubation was 5.5, whereas that for mannitol production in the second 24 h of incubation was 4.5. to achieve high cell density and mannitol yield simultaneously, a dual-stage ph control strategy was proposed based on the kinetic ...201323624845
the effect of starvation stress on lactobacillus brevis l62 protein profile determined by de novo sequencing in positive and negative mass spectrometry ion mode.we describe a novel negative chemically activated fragmentation/positive chemically activated fragmentation (caf-/caf+) technique for protein identification. the technique was used to investigate lactobacillus brevis adaptation to nutrient deprivation.201323592208
microdroplet-based multiplex pcr on chip to detect foodborne bacteria producing biogenic amines.the development of fast, reliable and culture-independent molecular tools to detect bacteria producing biogenic amines deserves the attention of research and ultimately of the food industry in order to protect consumers' health. here we present the application of a simple, low-cost, fast and sensitive method to perform microdroplet-based multiplex pcr, directly on a food matrix, for the simultaneous detection of bacterial genes involved in biogenic amine biosynthesis. after inoculating wine with ...201323628608
radio-protective effect of polysaccharides isolated from lactobacillus brevis-fermented ecklonia cava.we investigated the radioprotective effects of a polysaccharide isolated from enzymatic extracts of ecklonia cava (e. cava) fermented by fungi and bacteria. we identified that the aqueous extract of the lactobacillus brevis-fermented e. cava especially showed the highest proliferation effect. in addition, the enzymatic extract prepared by enzyme-assisted extraction using viscozyme (vlfe) significantly increased cell proliferation. further study indicated that the polysaccharides isolated from th ...201323068138
bacterial community dynamics, lactic acid bacteria species diversity and metabolite kinetics of traditional romanian vegetable fermentations.artisanal vegetable fermentations are very popular in eastern european countries. fresh vegetables undergo a spontaneous fermentation in the presence of salt, which is mainly carried out by lactic acid bacteria (lab).201322806635
effect of lactobacillus brevis kb290 on the cell-mediated cytotoxic activity of mouse splenocytes: a dna microarray analysis.lactic acid bacteria confer a variety of health benefits. here, we investigate the mechanisms by which lactobacillus brevis kb290 (kb290) enhances cell-mediated cytotoxic activity. female balb/c mice aged 9 weeks were fed a diet containing kb290 (3 × 10(9) colony-forming units/g) or starch for 1 d. the resulting cytotoxic activity of splenocytes against yac-1 cells was measured using flow cytometry and analysed for gene expression using dna microarray technology. kb290 enhanced the cell-mediated ...201323544404
genomic analysis by deep sequencing of the probiotic lactobacillus brevis kb290 harboring nine plasmids reveals genomic stability.we determined the complete genome sequence of lactobacillus brevis kb290, a probiotic lactic acid bacterium isolated from a traditional japanese fermented vegetable. the genome contained a 2,395,134-bp chromosome that housed 2,391 protein-coding genes and nine plasmids that together accounted for 191 protein-coding genes. kb290 contained no virulence factor genes, and several genes related to presumptive cell wall-associated polysaccharide biosynthesis and the stress response were present in l. ...201323544154
optimization of matrix-assisted-laser-desorption-ionization-time-of-flight mass spectrometry for the identification of bacterial contaminants in beverages.the growth of microbial contaminants in industrially produced beverages can cause turbidity, haze and off-flavors resulting in quality loss often rendering the product undrinkable. in this work matrix-assisted-laser-desorption-ionization-time-of-flight mass spectrometry (maldi-tof ms) based on the generation of peptide mass fingerprints, which form a distinctive protein peak pattern, is presented as a rapid, reliable and powerful tool for the identification of spoilage bacteria encountered in be ...201323541955
a description of the lactic acid bacteria microbiota associated with the production of traditional fermented vegetables in vietnam.an important part of the daily nourishment in vietnam constitutes of fermented vegetables. bacteria and especially lactic acid bacteria play a central role in the production of many fermented vegetables. the current study was conducted to investigate the diversity of native lactic acid bacteria (lab) populations in 'dua muoi' (mustard and beet fermentation) and 'ca muoi' (eggplant fermentation), three types of popular traditional fermented vegetables of vietnamese origin. to this end a polyphasi ...201323500611
characterization of lactobacillus brevis l62 strain, highly tolerant to copper ions.lactic acid bacteria (lab) as starter culture in food industry must be suitable for large-scale industrial production and possess the ability to survive in unfavorable processes and storage conditions. approaches taken to address these problems include the selection of stress-resistant strains. in food industry, lab are often exposed to metal ions induced stress. the interactions between lab and metal ions are very poorly investigated. because of that, the influence of non-toxic, toxic and antio ...201322927032
molecular identification of lactobacillus spp. associated with puba, a brazilian fermented cassava food.puba or carimã is a brazilian staple food obtained by spontaneous submerged fermentation of cassava roots. a total of 116 lactobacilli and three cocci isolates from 20 commercial puba samples were recovered on de man, rogosa and sharpe agar (mrs); they were characterized for their antagonistic activity against foodborne pathogens and identified taxonomically by classical and molecular methods. in all samples, lactic acid bacteria were recovered as the dominant microbiota (7.86 ± 0.41 log10 cfu/g ...201324159278
design and characterization of an efficient cyp105a1-based whole-cell biocatalyst for the conversion of resin acid diterpenoids in permeabilized escherichia coli.cytochrome p450 enzymes exhibit a tremendous potential for biotechnological applications due to their ability to introduce oxygen into non-activated carbon atoms. their catalytic diversity is complemented by a broad substrate range covering many natural compounds. especially the functionalization of terpenoids by p450s becomes increasingly interesting due to the diverse biological effects of these compounds. the bacterial cyp105a1 from streptomyces griseolus was recently identified to carry out ...201323793341
alleviating effects of lactobacillus strains on pathogenic vibrio parahaemolyticus-induced intestinal fluid accumulation in the mouse model.the aim of this study was to evaluate the probiotic effects of lactobacillus strains against vibrio parahaemolyticus causing gastroenteritis. six-week-old icr mice were pretreated with four lactobacillus strains at three dosages, and then challenged with v. parahaemolyticus tgqx01 (serotype o3:k6). the results showed that v. parahaemolyticus tgqx01 caused severe intestinal fluid accumulation (fa) and villi damage in control mice which were pretreated with phosphate-buffered saline. in contrast, ...201323210909
strain-specific probiotics properties of lactobacillus fermentum, lactobacillus plantarum and lactobacillus brevis isolates from brazilian food products.a total of 234 lab isolates from brazilian food products were initially screened for their ability to survive at ph 2.0. fifty one of the isolates survived and were selected. they were characterized by phenotypic methods, rep-pcr and identified using 16s rrna gene sequencing as lactobacillus fermentum (34 isolates), lactobacillus plantarum (10) and lactobacillus brevis (7). based on being either highly tolerant to bile, showing an ability for auto-aggregation and/or hydrophobic properties, one l ...201323764216
expression and characterization of a glutamate decarboxylase from lactobacillus brevis 877g producing γ-aminobutyric acid.the glutamate decarboxylase of γ-aminobutyric acid-producing lactobacillus brevis 877g (lbgad) was expressed in escherichia coli. the optimal ph and temperature for the purified lbgad activity were respectively determined to be ph 5.2 and 45 °c. cacl2 was shown to be a potent activator of this lbgad activity. the kinetic parameters for lbgad were a km value of 3.6 mmol/l and a vmax value of 0.06 mmol/l/min for l-monosodium glutamate.201323563537
biosynthesis of 3-hydroxypropionic acid from glycerol in recombinant escherichia coli expressing lactobacillus brevis dhab and dhar gene clusters and e. coli k-12 aldh.3-hydroxypropionic acid (3-hp) is a value-added chemical for polymer synthesis. for biosynthesis of 3-hp from glycerol, two dhab and dhar clusters encoding glycerol dehydratase and its reactivating factor, respectively, were cloned from lactobacillus brevis kctc33069 and expressed in escherichia coli. coexpression of dhab and dhar allowed the recombinant e. coli to convert glycerol to 3-hydroxypropionaldehyde, an intermediate of 3-hp biosynthesis. to produce 3-hp from glycerol, fed-batch ferment ...201323246300
synthesis of enantiopure fluorohydrins using alcohol dehydrogenases at high substrate concentrations.the use of purified and overexpressed alcohol dehydrogenases to synthesize enantiopure fluorinated alcohols is shown. when the bioreductions were performed with adh-a from rhodococcus ruber overexpressed in e. coli, no external cofactor was necessary to obtain the enantiopure (r)-derivatives. employing lactobacillus brevis adh, it was possible to achieve the synthesis of enantiopure (s)-fluorohydrins at a 0.5 m substrate concentration. furthermore, due to the activated character of these substra ...201323796348
effect of lactobacillus brevis 119-2 isolated from tsuda kabu red turnips on cholesterol levels in cholesterol-administered rats.in a previous in vitro study, we reported that the potential mechanism of the cholesterol-lowering effect of lactobacillus brevis 119-2 isolated from turnip tsuda kabu was the incorporation of cholesterol to cell membrane. in this study, we analyzed serum cholesterol and hepatic gene expression of sprague-dawley (sd) rats kept on a cholesterol diet with or without l. brevis 119-2 for 2 weeks, to evaluate the cholesterol-lowering effect in vivo. serum cholesterol levels were significantly reduced ...201323415664
removal of 3-methylindole by lactic acid bacteria in vitro.3-methylindole (3mi) is a substance with an unpleasant odor that is found in intact male pigs and is known to negatively affect consumers of pork. the growth of four strains of lactic acid bacteria [lactobacillus brevis 1.12 (l. brevis 1.12), l. plantarum 102, l. casei 6103 and l. plantarum atcc8014] in incubation medium with 3mi was studied. the four strains were tested for their ability to remove 3mi from the medium. the growth of l. brevis 1.12 remained steady as the levels of 3mi increased 3 ...201324137302
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