Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| gene cloning, functional expression and secretion of the s-layer protein sgse from geobacillus stearothermophilus nrs 2004/3a in lactococcus lactis. | the ~93-kda surface layer protein sgse of geobacillus stearothermophilus nrs 2004/3a forms a regular crystalline array providing a nanopatterned matrix for the future display of biologically relevant molecules. lactococcus lactis nz9000 was established as a safe expression host for the controlled targeted production of sgse based on the broad host-range plasmid pnz124sph, into which the nisa promoter was introduced. sgse devoid of its signal peptide-encoding sequence was cloned into the new vect ... | 2005 | 15675069 |
| microbiological study of lactic acid fermentation of caper berries by molecular and culture-dependent methods. | fermentation of capers (the fruits of capparis sp.) was studied by molecular and culture-independent methods. a lactic acid fermentation occurred following immersion of caper berries in water, resulting in fast acidification and development of the organoleptic properties typical of this fermented food. a collection of 133 isolates obtained at different times of fermentation was reduced to 75 after randomly amplified polymorphic dna (rapd)-pcr analysis. isolates were identified by pcr or 16s rrna ... | 2005 | 16332762 |
| lactic acid bacteria and yeasts associated with spontaneous fermentations during the production of sour cassava starch in brazil. | sour cassava starch is a traditional fermented food used in the preparation of fried foods and baked goods such as traditional cheese breads in brazil. thirty samples of sour cassava starch were collected from two factories in the state of minas gerais. the samples were examined for the presence of lactic acid bacteria, yeasts, mesophilic microorganisms, bacillus cereus and faecal coliforms. lactic acid bacteria and yeasts isolates were identified by biochemical tests, and the identities were co ... | 2005 | 16153731 |
| effect of protective agents, freezing temperature, rehydration media on viability of malolactic bacteria subjected to freeze-drying. | the effects of protective agents, rehydration media and freezing temperature on the viabilities of lactobacillus brevis and oenococcus oeni h-2 when subjected to freeze-drying were investigated. | 2005 | 16033464 |
| production of xylitol from d-xylose by recombinant lactococcus lactis. | the d-xylose reductase from pichia stipitis cbs 5773 and the xylose transporter from lactobacillus brevis atcc 8287 were expressed in active form in lactococcus lactis nz9800. xylitol production was investigated using non-growing recombinant cells in high cell-density under microaerobic conditions in the presence of xylose and glucose. besides xylose, the recombinant strain with xylose reductase activity reduced l-arabinose and d-ribose in significant extent to the corresponding pentitols. the r ... | 2005 | 15916828 |
| cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlk from lactobacillus brevis. | the commercial production of mannitol involves high-pressure hydrogenation of fructose using a nickel catalyst, a costly process. mannitol can be produced through fermentation by microorganisms. currently, a few lactobacillus strains are used to develop an efficient process for mannitol bioproduction; most of the strains produce mannitol from fructose with other products. an approach toward improving this process would be to genetically engineer lactobacillus strains to increase fructose-to-mann ... | 2005 | 15917616 |
| identification of predominant lactic acid bacteria isolated from traditionally fermented vegetable products of the eastern himalayas. | gundruk, sinki and khalpi are lactic-fermented vegetable products of sikkim in india, and inziangsang is a fermented leafy vegetable product of nagaland and manipur in india. a total of 65 samples of gundruk (25), sinki (12), khalpi (25) and inziangsang (3) were analysed for microbial counts. the population of lactic acid bacteria (lab) as well as aerobic mesophilic counts were at the level of 10(7) cfu g(-1). yeasts were detected only in few samples of sinki and khalpi. no moulds were detected. ... | 2005 | 16055218 |
| identification of potential probiotic starter cultures for scandinavian-type fermented sausages. | potential probiotic cultures suitable as starter cultures for the scandinavian-type fermented sausages were identified among strains well-adapted to fermented meats as well as strains originating from a culture collection. from 15 different fermented meat products, 22 strains were isolated as dominant non-starter lactic acid bacteria (nslab). the isolates were identified by rapd, api and sequence analysis of 16s rrna and showed to be five strains of lactobacillus sakei, five strains of lactobaci ... | 2005 | 16076509 |
| diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of gari, a traditional african food. | traditional fermentation of cassava is dominated by a lactic acid bacteria (lab) population. fermentation is important for improving product flavour and aroma as well as safety, especially by reduction of its toxic cyanogenic glucosides. the production of gari from cassava in benin typically occurs on a household or small industrial scale, and consequently suffers from inconsistent product quality and may not always be safe for consumption. therefore, the diversity of lab from a typical cassava ... | 2005 | 16104351 |
| optimization of adsorptive immobilization of alcohol dehydrogenases. | in this work, a systematic examination of various parameters of adsorptive immobilization of alcohol dehydrogenases (adhs) on solid support is performed and the impact of these parameters on immobilization efficiency is studied. depending on the source of the enzymes, these parameters differently influence the immobilization efficiency, expressed in terms of residual activity and protein loading. residual activity of 79% was achieved with adh from bakers' yeast (yadh) after optimizing the immobi ... | 2005 | 16233799 |
| resistance to antimicrobial agents in lactobacilli isolated from caper fermentations. | a collection of lactobacilli comprising species of lactobacillus plantarum (43 isolates), lactobacillus brevis (9 isolates) and lactobacillus fermentum (6 isolates) obtained from spontaneous fermentations of capers (the fruits of capparis spinosa) were investigated for resistance to antimicrobial agents. all isolates were resistant to vancomycin and teicoplanin (mic > 16 microg/ml). resistance to ciprofloxacin (mic > 2 microg/ml) was detected in all isolates of l. brevis and l. fermentum as well ... | 2005 | 16284934 |
| characterization of lactobacillus strains and monitoring by rapd-pcr in controlled fermentations of "almagro" eggplants. | the characterization of 23 lactobacillus strains was performed. the strains were assayed for biogenic amine-forming capacity, hydrogen peroxide production, pectin esterase, cellulase and polygalacturonase production, growth rate, acidifying capacity and salt tolerance. three strains were selected which belonged to the species, lactobacillus brevis, lactobacillus plantarum and lactobacillus fermentum. different starter cultures prepared as combinations of these three strains were assayed in pilot ... | 2005 | 15979183 |
| crystallization and preliminary crystallographic analysis of a flavoprotein nadh oxidase from lactobacillus brevis. | nadh oxidase (nox) from lactobacillus brevis is a homotetrameric flavoenzyme composed of 450 amino acids per subunit. the molecular weight of each monomer is 48.8 kda. the enzyme catalyzes the oxidation of two equivalents of nadh and reduces one equivalent of oxygen to yield two equivalents of water, without releasing hydrogen peroxide after the reduction of the first equivalent of nadh. crystals of this protein were grown in the presence of 34% polyethylene glycol monomethyl ether 2000, 0.1 m s ... | 2005 | 16511087 |
| [viability of a native isolate of lactobacillus brevis in a fermented milky drink]. | the objective of this research was to test the viability of a native isolate of lactobacillus brevis in two fermented milky drinks: a drink without prebiotic ingredient and another drink with oat flour (0.5%, p/v) as prebiotic ingredient. the viability of l. brevis was tested at 7, 14, and 21 after inoculation. drink ph and lactic acid concentration was measured as a function of time (2, 7, 14, and 21 days after inoculation). the isolate was viable in both drinks because its counting plates was ... | 2005 | 16640198 |
| horc confers beer-spoilage ability on hop-sensitive lactobacillus brevis abbc45cc. | to determine whether horc confers beer-spoilage ability and to evaluate the validity of horc as a trans-species genetic marker for differentiating the beer-spoilage ability of lactic acid bacteria (lab). | 2006 | 16696675 |
| usefulness of length heterogeneity-pcr for monitoring lactic acid bacteria succession during maize ensiling. | the use of length-heterogeneity pcr was explored to monitor lactic acid bacteria succession during ensiling of maize. bacterial diversity was studied during the fermentation of 30-day-old maize in optimal and spoilage-simulating conditions. a length heterogeneity pcr profile database of lactic acid bacteria isolated from the silage and identified by 16s rrna gene sequencing was established. although interoperonic 16s rrna gene length polymorphisms were detected in some isolates, strain analysis ... | 2006 | 16542413 |
| reclassification of lactobacillus brevis strains lmg 11494 and lmg 11984 as lactobacillus parabrevis sp. nov. | a polyphasic study revealed taxonomic heterogeneity among reference strains of the species lactobacillus brevis. representative strains of l. brevis and related taxa were investigated by partial sequence analysis of the housekeeping gene encoding the alpha-subunit of phenylalanyl-trna synthase (phes). species-specific clusters were delineated for all taxa studied except for two l. brevis strains, lmg 11494 and lmg 11984, respectively isolated from cheese and wheat, which occupied a distinct posi ... | 2006 | 16825629 |
| culture-independent analysis of the microbial composition of the african traditional fermented foods poto poto and dégué by using three different dna extraction methods. | the microbial composition of the traditional fermented foods poto poto (a maize dough from the rep. of congo) and dégué (a millet dough from burkina faso) was studied by a culture-independent approach using ttge to separate the amplified target v3 region of the 16s rrna gene from total microbial community, followed by dna sequencing and homology search. three different extraction methods were used. guanidium thiocyanate-based dna extraction provided better performance regarding purity and dna yi ... | 2006 | 16842876 |
| rapid discrimination of lactobacilli isolated from kefir grains by ft-ir spectroscopy. | fourier transform infrared (ft-ir) spectroscopy was used in combination with multivariate statistical analysis for differentiation of lactic bacteria isolated from kefir grains. twelve reference strains and 42 lactobacilli isolates from four local kefir grains, previously identified by biochemical traditional techniques at species level were included in this study. the spectra were analysed by hierarchical clustering analysis (hca) using pearson's product-moment correlation coefficient and ward' ... | 2006 | 16860422 |
| resident lactic acid bacteria in raw milk canestrato pugliese cheese. | investigation of the autochthonous lactic acid bacteria (lab) population of the raw milk protected designation of origin canestrato pugliese cheese using phenotypic and genotypic methodologies. | 2006 | 16869899 |
| influence of water-miscible organic solvents on kinetics and enantioselectivity of the (r)-specific alcohol dehydrogenase from lactobacillus brevis. | using the organic solvents acetonitrile and 1,4-dioxane as water-miscible additives for the alcohol dehydrogenase (adh)-catalyzed reduction of butan-2-one, we investigated the influence of the solvents on enzyme reaction behavior and enantioselectivity. the nadp(+)-dependent (r)-selective adh from lactobacillus brevis (adh-lb) was chosen as biocatalyst. for cofactor regeneration, the substrate-coupled approach using propan-2-ol as co-substrate was applied. acetonitrile and 1,4-dioxane were teste ... | 2006 | 16892294 |
| enhancement of gamma-aminobutyric acid production in chungkukjang by applying a bacillus subtilis strain expressing glutamate decarboxylase from lactobacillus brevis. | for a foreign glutamate decarboxylase (gad) to be expressed in bacillus host system, a recombinant dna (plip/lbgad) was constructed by ligating an lbgad gene from lactobacillus brevis opk-3 into escherichia coli-bacillus shuttle vector, plip. the plip/lbgad construct was then transformed into bacillus subtilis. the culture of the transformed bacillus strain with the plip/lbgad construct had higher gad activity and gamma-aminobutyric acid (gaba) concentration than those of untransformed bacillus ... | 2006 | 16955351 |
| characterization of a highly hop-resistant lactobacillus brevis strain lacking hop transport. | resistance to hops is a prerequisite for lactic acid bacteria to spoil beer. in this study we analyzed mechanisms of hop resistance of lactobacillus brevis at the metabolism, membrane physiology, and cell wall composition levels. the beer-spoiling organism l. brevis tmw 1.465 was adapted to high concentrations of hop compounds and compared to a nonadapted strain. upon adaptation to hops the metabolism changed to minimize ethanol stress. fructose was used predominantly as a carbon source by the n ... | 2006 | 17021196 |
| lactobacilli lozenges in the management of oral ulcers of behçet's syndrome. | there is increasing evidence that lactobacilli, having anti-inflammatory activity, may be useful in some diseases, particularly in inflammatory bowel disease. | 2006 | 17067433 |
| the mechanism of the tyrosine transporter tyrp supports a proton motive tyrosine decarboxylation pathway in lactobacillus brevis. | the tyrosine decarboxylase operon of lactobacillus brevis ioeb9809 contains, adjacent to the tyrosine decarboxylase gene, a gene for tyrp, a putative tyrosine transporter. the two genes potentially form a proton motive tyrosine decarboxylation pathway. the putative tyrosine transporter gene of l. brevis was expressed in lactococcus lactis and functionally characterized using right-side-out membranes. the transporter very efficiently catalyzes homologous tyrosine-tyrosine exchange and heterologou ... | 2006 | 16513749 |
| study on mesophilic and thermophilic alcohol dehydrogenases in gas-phase reaction. | the initial reaction rate and the thermostability of the mesophilic alcohol dehydrogenase (adh) from lactobacillus brevis (lbadh), and the thermophilic adh from thermoanaerobacter sp. (adh t) in gas-phase reaction were compared. the effects of water activity, cofactor-to-protein molar ratio, and reaction temperature on the reduction of acetophenone to 1-phenylethanol were studied. an optimal water activity of 0.55 in terms of productivity was found for both adhs. the cofactor-to-protein molar ra ... | 2006 | 16599562 |
| binding characteristics of the lactobacillus brevis atcc 8287 surface layer to extracellular matrix proteins. | self-assembling proteins that form crystalline surface layers on many microorganisms can be involved in bacterial-host adhesion via specific interactions with components of the extracellular matrix. here, we describe the interaction of the lactobacillus brevis atcc 8287 surface-layer protein slpa with fibronectin, laminin, fibrinogen and collagen using surface plasmon resonance. slpa was found to interact with high affinity to fibronectin and laminin, with a respective binding constant of 89.8 a ... | 2006 | 16842346 |
| performance assessment of malolactic fermenting bacteria oenococcus oeni and lactobacillus brevis in continuous culture. | the growth performance of malolactic fermenting bacteria oenococcus oeni ncimb 11648 and lactobacillus brevis x(2) was assessed in continuous culture. o. oeni grew at a dilution rate range of 0.007 to 0.052 h(-1) in a mixture of 5:6 (g l(-1)) of glucose/fructose at an optimal ph of 4.5, and l. brevis x(2) grew at 0.010 to 0.089 h(-1) in 10 g l(-1) glucose at an optimal ph of 5.5 in a simple and safe medium. the cell dry weight, substrate uptake and product formation were monitored, as well as gr ... | 2006 | 16012836 |
| new convenient defined media for [(35)s]methionine labelling and proteomic analyses of probiotic lactobacilli. | to develop experimental conditions for efficient protein radiolabelling and two-dimensional (2d) polyacrylamide gel electrophoresis for investigation of stress proteomes of probiotic lactobacillus spp. | 2006 | 16478505 |
| a multifactorial design for studying factors influencing growth and tyramine production of the lactic acid bacteria lactobacillus brevis cect 4669 and enterococcus faecium bifi-58. | a central composite face design was used to study growth and tyramine production of two strains of lactic acid bacteria, lactobacillus brevis cect 4669 and enterococcus faecium bifi-58. the effects of five physicochemical factors (incubation temperature and time, environmental ph, added tyrosine concentration, and pyridoxal-5-phosphate (plp) supplementation) on cell growth and tyramine production were analyzed under aerobic and anaerobic conditions. the parameters of the quadratic model for each ... | 2006 | 16488576 |
| production of biogenic amines by lactic acid bacteria: screening by pcr, thin-layer chromatography, and high-performance liquid chromatography of strains isolated from wine and must. | biogenic amines are frequently found in wine and other fermented food. we investigated the ability of 133 strains of lactic acid bacteria isolated from musts and wines of different origins to produce histamine, tyramine, and putrescine. we detected the genes responsible for encoding the corresponding amino acid decarboxylases through pcr assays using two primer sets for every gene: histidine decarboxylase (hdc), tyrosine decarboxylase (tdc), and ornithine decarboxylase (odc); these primers were ... | 2006 | 16496581 |
| characterization of hora and its flanking regions of pediococcus damnosus abbc478 and development of more specific and sensitive hora pcr method. | to characterize hora and its flanking regions of pediococcus damnosus abbc478 and, on the basis of this insight, to develop a more specific and sensitive hora pcr method. | 2006 | 16599994 |
| use of real time pcr to determine population profiles of individual species of lactic acid bacteria in alfalfa silage and stored corn stover. | real-time polymerase chain reaction (rt-pcr) was used to quantify seven species of lactic acid bacteria (lab) in alfalfa silage prepared in the presence or absence of four commercial inoculants and in uninoculated corn stover harvested and stored under a variety of field conditions. species-specific pcr primers were designed based on reca gene sequences. commercial inoculants improved the quality of alfalfa silage, but species corresponding to those in the inoculants displayed variations in pers ... | 2006 | 16205920 |
| [study on application of rep-pcr fingerprint in rapid identification of beer-spoilager]. | the application potential of rep-pcr in typing beer-spoilage isolates was studied. the effects of different factors, including dna templates and primers, on the quality and reproducibility of fingerprints were investigated. the ctab protocol was shown to be the feasible method for dna extraction. primers boxa1r and (gtg)5 were used in rep-pcr, and the pcr products were sequenced to identify strains isolated from two breweries. rep-pcr fingerprint profiles were obtained by using gelcompar ii soft ... | 2006 | 17168329 |
| identification of lactic acid bacteria isolated from kimchi and studies on their suitability for application as starter culture in the production of fermented sausages. | the aim of the investigation was to identify strains of lactobacilli coming from kimchi with properties suitable for use as starter cultures in sausage fermentation. a total of 31 strains of lactobacilli were isolated from kimchi on the 4-6th day of fermentation at 20°c using mrs agar plates and identified on the basis of morphological, biochemical, and physiological characteristics. the isolates were identified as leuconostoc mes.mes./dent (12.9%), lactobacillus curvatus (9.7%), lactobacillus b ... | 2006 | 22061727 |
| efficacy of reca gene sequence analysis in the identification and discrimination of lactobacillus hilgardii strains isolated from stuck wine fermentations. | conventional phenotypic methods sometimes lead to misidentification of some heterofermentative wine lactobacilli such as lactobacillus hilgardii, lactobacillus buchneri, and lactobacillus brevis. we establish the specificity of 16s rdna sequencing in the differentiation of these species and in the rejection of the lactobacillus vermiforme species name. moreover, we succeeded in differentiating these heterofermentative species by means of reca gene sequence comparison. short homologous regions we ... | 2007 | 17174001 |
| production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. | yogurt with high levels of gamma-aminobutyric acid (gaba), free amino acids and isoflavones was developed using lactic acid bacteria (lab) and germinated soybean extract. fermented soya milk (gaba soya yogurt) produced with starter and substrate had the gaba concentration of 424.67 microg/gdw, whereas fermented milk produced by a conventional method had gaba less than 1.5 microg/gdw. the gaba soya yogurt also contained significantly high levels of free amino acids and isoflavones compared with o ... | 2007 | 17055264 |
| analysis of vaginal lactic acid producing bacteria in healthy women. | vaginal lactic acid-producing bacteria of 80 pre-menopausal women were studied by isolation on blood and deman-rogosa-sharpe agar, pcr with group-specific primers for lactobacillus-denaturing gradient gel electrophoresis (dgge), and pcr with specific primers for v3 region in 16s rrna-temporal temperature gel electrophoresis (ttge). conventional isolation method on media detected only one lactobacillus (lactobacillus brevis) while ttge detected only lactobacillus sp. dgge detected seven lactobaci ... | 2007 | 18176534 |
| characterization of microflora in homemade semi-hard white zlatar cheese. | the zlatar cheese belongs to the group of traditionally homemade cheeses, which are produced from nonpasteurized cow's milk, without adding of any bacterial starter culture. changes were followed in lactic acid bacteria population and chemical composition during the ripening period of cheese up to 60 days. results showed that the percentage of lactic acid cocci was higher in raw milk and one day old cheese and their percentage was gradually decreasing, whereas the number of lactobacilli was incr ... | 2007 | 17182146 |
| enterococcus and lactobacillus contamination of raw milk in a farm dairy environment. | enterococci and lactobacilli are ubiquitously found in the intestinal microflora of humans and animals. the aim of the present study was to determine the importance of bovine faeces as a source of these organisms in raw milk. one hundred and fifty six putative enterococci and 362 lactobacilli were isolated from bovine faeces (n=26), cows' teats, raw milk, the milking machine and the milking environment on one farm. the clonal relationships of each group were investigated using pulsed-field gel e ... | 2007 | 17189657 |
| characterization of liposomes coated with s-layer proteins from lactobacilli. | the stability of liposomes coated with s-layer proteins from lactobacillus brevis and lactobacillus kefir was analyzed as a previous stage to the development of a vaccine vehicle for oral administration. the interactions of the different s-layer proteins with positively charged liposomes prepared with soybean lecithin or dipalmitoylphosphatidylcholine were studied by means of the variation of the z potential at different protein-lipid ratios, showing that both proteins were able to attach in a g ... | 2007 | 17276386 |
| lactobacillus plantarum for oral peptide delivery. | to evaluate strains of lactobacilli for their ability to persist and secrete heterologous protein in the oral cavity. | 2007 | 17311639 |
| preliminary characterization of lactic acid bacteria isolated from zlatar cheese. | isolation, characterization and identification of lactic acid bacteria (lab) from artisanal zlatar cheese during the ripening process and selection of strains with good technological characteristics. | 2007 | 18045397 |
| characterization of italian cheeses ripened under nonconventional conditions. | four italian cheeses (casciotta di urbino, barricato san martino, vento d'estate, and ubriaco di raboso) nonconventionally ripened under different plant materials (walnut leaves, herbs, hay, and wine by-products, respectively) were compared for compositional, microbiological, biochemical, and volatile profile characteristics. mean values for gross composition were rather similar. because primary starters were not used for manufacture, the endogenous lactic acid bacteria were mainly present (7.0 ... | 2007 | 17517708 |
| cell-cell communication in sourdough lactic acid bacteria: a proteomic study in lactobacillus sanfranciscensis cb1. | the mechanisms of cell-cell communication in lactobacillus sanfranciscensis cb1 were studied. the highest number of dead/damaged cells of l. sanfranciscensis cb1 was found in cocultures with lactobacillus plantarum dc400 or lactobacillus brevis cr13 when the late stationary phase of growth (18 h) was reached. 2-de analysis was carried out. almost the same proteins were induced in all three cocultures at the mid-exponential phase of growth (7 h). the number of induced proteins markedly increased ... | 2007 | 17623302 |
| coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in lactobacillus brevis. | lactobacillus brevis atcc367 was engineered to express pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) genes in order to increase ethanol fermentation from biomass-derived residues. first, a gram-positive sarcina ventriculi pdc gene (svpdc) was introduced into l. brevis atcc 367 to obtain l. brevis bbc03. the svpdc was detected by immunoblot using an svpdc oligo peptide antiserum, but no increased ethanol was detected in l. brevis bbc03. then, an adh gene from l. brevis (bradh) was ... | 2007 | 17651394 |
| increased internal and external bacterial load during drosophila aging without life-span trade-off. | the role of microbial load during aging of the adult fruit fly drosophila melanogaster is incompletely understood. here we show dramatic increases in aerobic and anaerobic bacterial load during aging, both inside the body and on the surface. scanning electron microscopy and cell staining analyses of the surface of aged flies detected structures resembling abundant small bacteria and bacterial biofilms. bacteria cultured from laboratory flies included aerobic species acetobacter aceti, acetobacte ... | 2007 | 17681150 |
| use of sourdough lactobacilli and oat fibre to decrease the glycaemic index of white wheat bread. | this work was aimed at decreasing the glycaemic index (gi) of white wheat bread. breads made with wheat flour (wf) or wholemeal flour (wmf) and fermented with baker's yeast had similar values of resistant starch (rs; 1.4-1.7 %, starch basis). sourdough lactobacillus plantarum p1 and lactobacillus brevis p2 favoured the highest formation of rs (approximately 5 %) when fermented with wf and wmf. the mixture (1:1) of wf and wmf (wf/wmf) was selected. the effect of dietary fibres, chemical or sourdo ... | 2007 | 17697425 |
| continuous asymmetric ketone reduction processes with recombinant escherichia coli. | the reduction of methyl acetoacetate was carried out in continuously operated biotransformation processes catalyzed by recombinant escherichia coli cells expressing an alcohol dehydrogenase from lactobacillus brevis. three different cell types were applied as biocatalysts in three different cofactor regeneration approaches. both processes with enzyme-coupled cofactor regeneration catalyzed by formate dehydrogenase or glucose dehydrogenase are characterized by a rapid deactivation of the biocatal ... | 2007 | 17826859 |
| dna fingerprinting of lactic acid bacteria in sauerkraut fermentations. | previous studies using traditional biochemical identification methods to study the ecology of commercial sauerkraut fermentations revealed that four species of lactic acid bacteria, leuconostoc mesenteroides, lactobacillus plantarum, pediococcus pentosaceus, and lactobacillus brevis, were the primary microorganisms in these fermentations. in this study, 686 isolates were collected from four commercial fermentations and analyzed by dna fingerprinting. the results indicate that the species of lact ... | 2007 | 17921264 |
| characterization and selection of autochthonous lactic acid bacteria isolated from traditional iberian dry-fermented salchichón and chorizo sausages. | a total of 192 lactic acid bacteria were isolated from 2 types of naturally fermented dry sausages at 3 different stages of the ripening process in order to select the most suitable strains as starter cultures in dry-cured sausage manufacture according to their technological characteristics such as glucose fermentation, lactic and acetic acid production, and proteolytic, lipolytic, and antimicrobial activities. identification of the isolates revealed that 31.2% were pediococcus pentosaceus, 26.9 ... | 2007 | 17995686 |
| isolation of surface (s) layer protein carrying lactobacillus species from porcine intestine and faeces and characterization of their adhesion properties to different host tissues. | surface-layer proteins (slps) of lactobacilli have been shown to confer tissue adherence. this study aimed to isolate and identify slps carrying lactobacillus species from the porcine intestine and faeces and to characterize these s-layer-expressing strains for their ability to adhere to the pig and human intestinal cells and to extracellular matrix (ecm) proteins. in total 99 strains, putatively belonging to the genus lactobacillus, were isolated as pure cultures. sds-page and a gene probe spec ... | 2007 | 17544232 |
| anti-inflammatory effects of lactobacillus brevis (cd2) on periodontal disease. | to analyze the anti-inflammatory effects of lactobacillus brevis extracts on periodontitis patients and to investigate the involved mechanisms in vitro on activated macrophages. | 2007 | 17577323 |
| agmatine deiminase pathway genes in lactobacillus brevis are linked to the tyrosine decarboxylation operon in a putative acid resistance locus. | in lactic acid bacteria (lab), amino acids and their derivatives may be converted into amine-containing compounds designated biogenic amines, in pathways providing metabolic energy and/or acid resistance to the bacteria. in a previous study, a pathway converting tyrosine to tyramine was detected in lactobacillus brevis and a fragment of a gene possibly involved in the production of another biogenic amine, putrescine, from agmatine, was detected in the same locus. the present study was carried ou ... | 2007 | 17600066 |
| mathematical modelling of nadh oxidation catalyzed by new nadh oxidase from lactobacillus brevis in continuously operated enzyme membrane reactor. | nadh oxidase from lactobacillus brevis was kinetically characterized in two different buffers: tris-hcl and glycine-sodium pyrophosphate (ph 9.0). reaction kinetics was described using the michaelis-menten model with product (nad(+)) inhibition. it was found that this type of inhibition is uncompetitive. experiments in the continuously operated enzyme membrane reactor revealed a strong enzyme deactivation at two different residence times: 12 and 60 min. a stronger deactivation was observed at th ... | 2007 | 18023799 |
| proteomic approach for characterization of hop-inducible proteins in lactobacillus brevis. | resistance to hops is a prerequisite for the capability of lactic acid bacteria to grow in beer and thus cause beer spoilage. bactericidal hop compounds, mainly iso-alpha-acids, are described as ionophores which exchange h+ for cellular divalent cations, e.g., mn2+, and thus dissipate ion gradients across the cytoplasmic membrane. the acid stress response of lactobacillus brevis tmw 1.465 and hop adaptation in its variant l. brevis tmw 1.465a caused changes at the level of metabolism, membrane p ... | 2007 | 17369340 |
| effects of morphological changes in beer-spoilage lactic acid bacteria on membrane filtration in breweries. | membrane filter performance was investigated using beer-spoilage lactic acid bacteria (lab). as a result, beer-adapted lab strains showed considerably increased penetration rate through filters, as compared with non-adapted strains. further statistical analyses demonstrated the significant shifts in cell size distribution towards shorter rods, when lactobacillus brevis and l. lindneri strains were precultured in beer. these results indicate that diminished cell size is responsible for the deteri ... | 2007 | 18023809 |
| characterization of lactobacilli isolated from caper berry fermentations. | to determine the metabolic and functional properties of lactobacilli isolated from caper fermentation. | 2007 | 17241365 |
| cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, lactobacillus brevis opk-3. | lactobacillus brevis opk-3, having 84.292 mg/l/h of gamma-aminobutyric acid (gaba) productivity, was isolated from kimchi, a traditional fermented food in korea. a core fragment of glutamate decarboxylase (gad) dna was isolated from the l. brevis opk-3, using primers based on two highly conserved regions of gad. a full-length gad (lbgad) clone was subsequently isolated through rapid amplification of cdna ends (race) pcr. nucleotide sequence analysis revealed that the open reading frame (orf) con ... | 2007 | 16500100 |
| tyramine and phenylethylamine production among lactic acid bacteria isolated from wine. | the ability of wine lactic acid bacteria to produce tyramine and phenylethylamine was investigated by biochemical and genetic methods. an easy and accurate plate medium was developed to detect tyramine-producer strains, and a specific pcr assay that detects the presence of tdc gene was employed. all strains possessing the tdc gene were shown to produce tyramine and phenylethylamine. wines containing high quantities of tyramine and phenylethylamine were found to contain lactobacillus brevis or la ... | 2007 | 17307265 |
| characterization of pfmbl1, a small cryptic plasmid isolated from leuconostoc mesenteroides sy2. | a 4661bp cryptic plasmid, pfmbl1, was isolated from leuconostoc mesenteroides sy2, an isolate from kimchi, and characterized. nucleotide sequence analysis revealed two open reading frames, orf1 and orf2. orf2 was 453bp in size and its translation product had 58% identity with a putative protein possibly involved in the replication of ptxl1, a cryptic plasmid from l. mesenteroides ssp. mesenteroides y110. rna transcript from orf2 was detected but not from orf1 or intergenic region. minimum 3.5kb ... | 2007 | 17084452 |
| antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms. | non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ... | 2007 | 17049799 |
| synthesis of gamma-aminobutyric acid by lactic acid bacteria isolated from a variety of italian cheeses. | the concentrations of gamma-aminobutyric acid (gaba) in 22 italian cheese varieties that differ in several technological traits markedly varied from 0.26 to 391 mg kg(-1). presumptive lactic acid bacteria were isolated from each cheese variety (total of 440 isolates) and screened for the capacity to synthesize gaba. only 61 isolates showed this activity and were identified by partial sequencing of the 16s rrna gene. twelve species were found. lactobacillus paracasei pf6, lactobacillus delbruecki ... | 2007 | 17890341 |
| application of artificial neural network coupling particle swarm optimization algorithm to biocatalytic production of gaba. | the biotransformation of l-sodium glutamate (l-msg) to gamma-aminobutyric acid (gaba) catalyzed by the cells of lactobacillus brevis with higher glutamate decarboxylase activity was investigated. the results showed that ph, temperature, and feso(4) x 7h(2)o concentration had significantly positive effect on gaba yield. the individual and interactive effects of ph, temperature, and feso(4) x 7h(2)o concentration were further optimized in terms of gaba yield. in the present work, an artificial neu ... | 2007 | 16952178 |
| population dynamics and metabolite target analysis of lactic acid bacteria during laboratory fermentations of wheat and spelt sourdoughs. | four laboratory sourdough fermentations, initiated with wheat or spelt flour and without the addition of a starter culture, were prepared over a period of 10 days with daily back-slopping. samples taken at all refreshment steps were used for determination of the present microbiota. furthermore, an extensive metabolite target analysis of more than 100 different compounds was performed through a combination of various chromatographic methods including liquid chromatography-mass spectrometry and ga ... | 2007 | 17557853 |
| lactic acid bacteria in hamei and marcha of north east india. | hamei and marcha are mixed dough inocula used as starters for preparation of various indigenous alcoholic beverages in manipur and sikkim in india, respectively. these starters are traditionally prepared from rice with wild herbs and spices. samples of hamei and marcha, collected from manipur and sikkim, respectively, were analysed for lactic acid bacterial composition. the population of lactic acid bacteria (lab) was 6.9 and 7.1 log cfu/g in hamei and marcha, respectively. on the basis of pheno ... | 2007 | 23100653 |
| proteolysis in model portuguese cheeses: effects of rennet and starter culture. | to shed further light onto the mechanisms of proteolysis that prevail throughout ripening of portuguese cheeses, model cheeses were manufactured from bovine milk, following as much as possible traditional manufacture practices - using either animal or plant rennet. the individual role upon proteolysis of two (wild) strains of lactic acid bacteria - viz. lactococcus lactis and lactobacillus brevis, which are normally found to high viable numbers in said cheeses, was also considered, either as sin ... | 2008 | 26065746 |
| ionic liquids for aqueous two-phase extraction and stabilization of enzymes. | the ionic liquid (il) ammoeng110 contains cations with oligoethyleneglycol units and was found to be highly effective for the formation of aqueous two-phase systems (atps) that can be used for the biocompatible purification of active enzymes. above critical concentrations of the il and an inorganic salt in aqueous solution, phase separation takes place resulting in the formation of an il-enriched upper and a salt-enriched lower phase. for the optimization of the composition of il-based atps with ... | 2008 | 18023057 |
| oral administration of heat-killed lactobacillus brevis sbc8803 ameliorates the development of dermatitis and inhibits immunoglobulin e production in atopic dermatitis model nc/nga mice. | we have previously shown that the oral administration of heat-killed lactobacillus brevis (l. brevis) sbc8803 strain inhibits ige production in ovalbumin (ova)-sensitized balb/c mice through improvement of the type-1 helper t (th1)/th2 balance toward th1 dominance. atopic dermatitis is one of the most common skin diseases and is frequently associated with elevated immunoglobulin e (ige) antibodies against many kinds of allergens. in this study, we investigated the inhibitory effect of oral admin ... | 2008 | 18451512 |
| glutamate decarboxylase from lactobacillus brevis: activation by ammonium sulfate. | in this study, the glutamate decarboxylase (gad) gene from lactobacillus brevis ifo12005 (biosci. biotechnol. biochem., 61, 1168-1171 (1997)), was cloned and expressed. the deduced amino acid sequence showed 99.6% and 53.1% identity with gad of l. brevis atcc367 and l. lactis respectively. the his-tagged recombinant gad showed an optimum ph of 4.5-5.0, and 54 kda on sds-page. the gad activity and stability was significantly dependent on the ammonium sulfate concentration, as observed in authenti ... | 2008 | 18460820 |
| ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration. | wet brewers grains and soybean curd residue were stored in laboratory-scale silos without (bg and sc silages, respectively) or with other ingredients as total mixed rations (bgt and sct silages, respectively). silages were opened after 14 and 56 d, and microbial counts, fermentation products, and aerobic stability were determined. denaturing gradient gel electrophoresis was carried out to examine bacterial communities, and several bacteria that appeared to be involved in fermentation were identi ... | 2008 | 18487660 |
| rapid identification of the three major species of dairy obligate heterofermenters lactobacillus brevis, lactobacillus fermentum and lactobacillus parabuchneri by species-specific duplex pcr. | in this study, the biodiversity of 154 strains of lactic acid bacteria, including 112 dairy product isolates presumptively identified as obligately heterofermentative lactobacilli (ohl) by classical microbiological tests, as well as 23 ohl-type strains, was investigated by pcr-based methods and gene sequencing. using these techniques, 51% of the cheese isolates were actually identified as ohl. the non-ohl isolates were identified to the leuconostoc, lactobacillus, weisella, pediococcus or strept ... | 2008 | 18510560 |
| antimicrobial activity of enterococcus faecium l50, a strain producing enterocins l50 (l50a and l50b), p and q, against beer-spoilage lactic acid bacteria in broth, wort (hopped and unhopped), and alcoholic and non-alcoholic lager beers. | enterococcus faecium l50 produces enterocin l50 (l50a and l50b) (entl50, entl50a and entl50b), enterocin p (entp) and enterocin q (entq) and displays a broad antimicrobial spectrum against the most relevant beer-spoilage lactic acid bacteria (lab) (i.e., lactobacillus brevis and pediococcus damnosus), which is mainly due to the production of entl50 (entl50a and entl50b). bacteriocin assays using in vitro-synthesized entl50 (entl50a and entl50b) showed that both individual peptides possess antimi ... | 2008 | 18544465 |
| microflora of feta cheese from four greek manufacturers. | the components of the microflora of four feta cheeses, produced by different greek manufacturers, were determined by culture dependent and independent techniques. isolates from microbiological media were first grouped by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) and then representatives of each dgge group were sequenced for identification purposes. dna and rna, extracted directly from the cheese, were subjected to pcr-dgge. moreover, feta cheeses were subjected ... | 2008 | 18555549 |
| phenotypic and genotypic identification of lactic acid bacteria isolated from ethnic fermented bamboo tender shoots of north east india. | mesu, soidon, soibum and soijim are ethnic fermented bamboo tender shoot products prepared by the people in north east india. microbiological analysis of mesu, soidon, soibum and soijim showed the population dominated by lactic acid bacteria (lab) ranging up to 10(8) cfu g(-1). the phenotypic characterisation of predominant lab isolated from the fermented bamboo shoot products was based on general morphology, physiological tests, api and biolog systems. the genotypic characterisation of lab was ... | 2008 | 18036695 |
| a strategy to prevent the occurrence of lactobacillus strains using lactate-tolerant yeast candida glabrata in bioethanol production. | contamination of lactobacillus sp. in the fermentation broth of bioethanol production decreases ethanol production efficiency. although the addition of lactate to the broth can effectively inhibit the growth of lactobacillus sp., it also greatly reduces the fermentation ability of saccharomyces cerevisiae. to overcome this conflict, lactate-tolerant yeast strains were screened. candida glabrata strain nfri 3164 was found to exhibit both higher levels of lactate tolerance and fermentation ability ... | 2008 | 18597130 |
| estimation of 16s rrna gene copy number in several probiotic lactobacillus strains isolated from the gastrointestinal tract of chicken. | the copy numbers of 16s rrna genes in 12 probiotic lactobacillus strains of poultry origin were analyzed. genomic dna of the strains was digested with restriction endonucleases that do not cut within the 16s rrna gene of the strains. this was followed by southern hybridization with a biotinylated probe complementary to the 16s rrna gene. the copy number of the 16s rrna gene within a lactobacillus species was found to be conserved. from the hybridization results, lactobacillus salivarius i 24 was ... | 2008 | 18707622 |
| conjugated linoleic acid producing potential of lactobacilli isolated from the rumen of cattle. | lactobacilli isolated from the rumen of cattle were subjected to morphological and biochemical characterizations followed by pcr-based identification. among isolates, lactobacillus brevis was found to be the most prevalent species in the rumen. for in vitro conjugated linoleic acid (cla) production, the two isolates of l. brevis and one each of lactobacillus viridescens and lactobacillus lactis were selected. the sunflower oil (i.e., 0.25, 0.5 and 1.0%; a rich source of linoleic acid) was added ... | 2008 | 18712544 |
| diversity of lactic acid bacteria in two flemish artisan raw milk gouda-type cheeses. | pcr-denaturing gradient gel electrophoresis (pcr-dgge) was used to study the diversity of lactic acid bacteria (lab) in two flemish artisan raw milk gouda-type cheeses. in parallel, conventional culturing was performed. isolates were identified using (gtg)(5)-pcr and sequence analysis of 16s rrna and phes genes. discriminant analysis revealed some differences in overall lab diversity between the two batches and between the two cheeses. within each batch, the diversity of 8- and 12-week-old chees ... | 2008 | 18721684 |
| identification, cloning, and characterization of a novel ketoreductase from the cyanobacterium synechococcus sp. strain pcc 7942. | a new ketoreductase useful for asymmetric synthesis of chiral alcohols was identified in the cyanobacterium synechococcus sp. strain pcc 7942. mass spectrometry of trypsin-digested peptides identified the protein as 3-ketoacyl-[acyl-carrier-protein] reductase (kr) (ec 1.1.1.100). the gene, referred to as fabg, was cloned, functionally expressed in escherichia coli, and subsequently purified to homogeneity. the enzyme displayed a temperature optimum at 44 degrees c and a broad ph optimum between ... | 2008 | 18791006 |
| long-term fungal inhibitory activity of water-soluble extracts of phaseolus vulgaris cv. pinto and sourdough lactic acid bacteria during bread storage. | the antifungal activity of proteinaceous compounds from different food matrices was investigated. in initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with penicillium roqueforti dppmaf1 as the indicator fungus. water-soluble extracts of sourdough fermented with lactobacillus brevis am7 and phaseolus vulgaris cv. pinto were selected for further study. the crude water-soluble extracts of l. brevis am7 sourdough and p. v ... | 2008 | 18849463 |
| biofilm formation by lactic acid bacteria and resistance to environmental stress. | we investigated the formation of biofilms by 3 type strains of lactic acid bacteria (lab), lactobacillus plantarum, lactobacillus brevis and lactobacillus fructivorans, as representatives of lab that cause food deterioration or contamination. lactobacillus plantarum subsp. plantarum jcm1149 and lactobacillus brevis jcm1059 appeared to adhere and accumulate on glass cover slips. lactobacillus fructivorans jcm1117 cells made thin cellophane-like biofilms, and most of the biofilm cells became longe ... | 2008 | 19000615 |
| effects of initial air removal methods on microorganisms and characteristics of fermented plant beverages. | the effects of 3 different methods for removing the initial air on the properties of fermented plant beverages produced from phom-nang seaweed (gracilaria fisheri) and wild forest noni (morinda coreia ham.) were investigated. only method m which covered the space above the fermentation liquid with a water filled plastic bag produced no surface film of yeast, had the highest acidity and also antibacterial activity from both plants after 90 days of fermentation. however, the yeast count still exce ... | 2008 | 18817186 |
| identification and characterization of domains responsible for self-assembly and cell wall binding of the surface layer protein of lactobacillus brevis atcc 8287. | lactobacillus brevis atcc 8287 is covered by a regular surface (s-) layer consisting of a 435 amino acid protein slpa. this protein is completely unrelated in sequence to the previously characterized s-layer proteins of lactobacillus acidophilus group. | 2008 | 18828902 |
| oral administration of heat-killed lactobacillus brevis sbc8803 ameliorates alcoholic liver disease in ethanol-containing diet-fed c57bl/6n mice. | we examined the effect of heat-killed lactobacillus brevis (l. brevis) sbc8803 on the development of alcoholic liver disease using ethanol-containing diet-fed mice. heat-killed l. brevis was orally administered at a dose of 100 or 500 mg/kg once a day for 35 days. alcoholic liver injury was examined by measuring the activity of alanine aminotransferase (alt) and aspartate aminotransferase (ast) in a serum, and the alcoholic fatty liver was assessed from the content of triglyceride (tg) and total ... | 2008 | 18976829 |
| phenotypic and molecular identification of lactobacilli isolated from vaginal secretions. | twenty lactobacilli isolated from vaginal samples of healthy volunteers were characterized according to polyphasic taxonomy. a broad spectrum of activity and protective properties of these vaginal isolates has been reported. | 2008 | 19255690 |
| pcr-dgge analysis of lactic acid bacteria and yeast dynamics during the production processes of three varieties of panettone. | to study lactic acid bacteria (lab) and yeast dynamics during the production processes of sweet-leavened goods manufactured with type i sourdoughs. | 2008 | 18312562 |
| chemoenzymatic synthesis of the chiral side-chain of statins: application of an alcohol dehydrogenase catalysed ketone reduction on a large scale. | the chemoenzymatic synthesis of the tert-butyl (s)-6-chloro-5-hydroxy-3-ketohexanoate is described. our approach relies on a highly regio- and enantioselective reduction of a beta,delta-diketohexanoate ester catalysed by nadp(h)-dependent alcohol dehydrogenase of lactobacillus brevis (lbadh). a detailed description of the scale-up of the enzymatic synthesis of the hydroxyketo ester is given which includes a scale-up of the substrate synthesis as well, i.e. the preparation of diketo ester on a 10 ... | 2008 | 18288496 |
| probiotic properties of lactic acid bacteria isolated from stool samples of longevous people in regions of hotan, xinjiang and bama, guangxi, china. | a total of 567 lactic acid bacteria (lab) strains were isolated from the stool samples of longevous people in regions of hotan, xinjiang and bama, guangxi, china. in order to reduce the number of strains for further examinations, 36 isolates were screened out for further examination whilst the other strains, which had lower probiotic properties, were not suitable for yogurt production due to the absence of growth in ph 3.5 mrs medium and no curding during fermentation, and so were excluded. the ... | 2008 | 18634896 |
| effect of oral administration of heat-killed lactobacillus brevis sbc8803 on total and ovalbumin-specific immunoglobulin e production through the improvement of th1/th2 balance. | we examined the effect of 59 strains of heat-killed lactobacillus brevis on interleukin (il)-12 and interferon (ifn)-gamma production from mouse peyer's patch (pp) cells. l. brevis has a great variety of strains that induce the production of these cytokines. some l. brevis strains, which were selected for their ability to induce a strong th1 immune response, inhibited both total immunoglobulin e (ige) and antigen specific ige production, and improved the th1/th2 balance by enhancing il-12 and if ... | 2008 | 18055049 |
| direct quantitation of fatty acids present in bacteria and fungi: stability of the cyclopropane ring to chlorotrimethylsilane. | the stability of the cyclopropane ring and the fatty acid composition of microbial cells were determined using chlorotrimethylsilane as reagent with three different conditions 80 degrees c for 1 h, 60 degrees c for 1 h, and 60 degrees c for 2 h. chlorotrimethylsilane permits a simultaneous extraction and derivatization of fatty acids. a basic method was used as reference. the bacteria, escherichia coli, burkholderia cepacia, and lactobacillus brevis, and fungi aspergillus niger and gibberella fu ... | 2008 | 18533660 |
| rapid detection and quantification of tyrosine decarboxylase gene (tdc) and its expression in gram-positive bacteria associated with fermented foods using pcr-based methods. | in this study, pcr-based procedures were developed to detect the occurrence and quantify the expression of the tyrosine decarboxylase gene (tdc) in gram-positive bacteria associated with fermented foods. consensus primers were used in conventional and reverse transcription pcr to analyze a collection of 87 pure cultures of lactic acid bacteria and staphylococci. all enterococci, staphylococcus epidermidis, lactobacillus brevis, lactobacillus curvatus, and lactobacillus fermentum strains and 1 of ... | 2008 | 18236668 |
| purification and gene cloning of a dehydrogenase from lactobacillus brevis that catalyzes a reaction involved in aflatoxin biosynthesis. | when 10 strains of lactic acid bacteria were incubated with 5'-hydroxyaverantin (havn), a precursor of aflatoxins, seven of them converted havn to averufin; the same reaction is found in aflatoxin biosynthesis of aflatoxigenic fungi. these bacteria had a dehydrogenase that catalyzed the reaction from havn to 5'-oxoaverantin (oavn), which was so unstable that it was easily converted to averufin. the enzyme was purified from lactobacillus brevis ifo 12005. the molecular mass of the enzyme was 100 ... | 2008 | 18323645 |
| diversity and dynamics of lactobacilli populations during ripening of rdo camembert cheese. | the diversity and dynamics of lactobacillus populations in traditional raw milk camembert cheese were monitored throughout the manufacturing process in 3 dairies. culture-dependent analysis was carried out on isolates grown on acidified de man - rogosa - sharpe agar and lactobacillus anaerobic de man rogosa sharpe agar supplemented with vancomycin and bromocresol green media. the isolates were identified by polymerase chain reaction - temperature gradient gel electrophoresis (pcr-tgge) and (or) ... | 2008 | 18388993 |
| molecular and technological characterization of lactic acid bacteria from traditional fermented sausages of basilicata region (southern italy). | lactic acid bacteria (lab) from traditional fermented sausages of the basilicata region were investigated by ardra-pcr and rapd-pcr for taxonomic identification at species and strain level and characterized on the basis of the growth and acidification at different temperatures, incubation times, levels of nacl and kno(2), hydrolysis of sarcoplasmatic and myofibrillar proteins and antimicrobial, peptide/amino acid release and nitrate reductase activities. lactobacillus sakei was the predominant s ... | 2008 | 22063864 |
| rapid differentiation of lactic acid bacteria from autochthonous fermentation of iberian dry-fermented sausages. | the populations of lactic acid bacteria (lab) in different types of iberian dry-fermented sausages from central-west spain were identified. a simple and rapid electrophoretic method of whole-cell protein profiles was evaluated, correlating it with 16s rrna gene sequence analysis and biochemical identification by api 50 chl. a total of 96 isolates were identified by sds-page showing stable profiles corresponding to 30-45 polypeptides in the range 95-8kda that were clearly different for the differ ... | 2008 | 22063578 |
| phenotypic and genetic heterogeneity of lactic acid bacteria isolated from "alheira", a traditional fermented sausage produced in portugal. | the aim of this study was to evaluate the phenotypic and genetic heterogeneity of lactic acid bacteria (lab) isolated from "alheira", a fermented sausage produced in portugal. lab were identified to genus and species level by phenotypic characteristics, using genus or species-specific primers and sequencing of the gene encoding 16s rrna. two-hundred and eighty-three isolates were grouped into 14 species. lactobacillus plantarum was isolated from all sausages and enterococcusfaecalis from most of ... | 2009 | 20416703 |
| bacterial dynamics in model cheese systems, aiming at safety and quality of portuguese-style traditional ewe's cheeses. | an experiment using model ewe's milk cheeses was designed to characterize microbial interactions that arise in actual raw milk cheese environments. these model cheeses were manufactured according to portuguese artisanal practices, except that the microbial load and biodiversity were fully controlled: single potential pathogens and spoilage bacteria, or a combination thereof, were combined at various initial inoculum levels in sterilized raw ewe's milk with several lactic acid bacteria (lab) norm ... | 2009 | 19903385 |
| cloning and expression of beta-glucuronidase from lactobacillus brevis in e. coli and application in the bioconversion of baicalin and wogonoside. | the beta-glucuronidase (gus) gene from lactobacillus brevis ro1 was cloned and expressed in escherichia coli gms407. the gus gene was composed of 1812 bp, encoding a 603-amino-acid protein belonging to the glycosyl hydrolase family 2 with three conserved domains. the amino acid similarity was higher than 70% with the beta-glucuronidases of various microorganisms, yet less than 58% with the beta-glucuronidase of l. gasseri adh. overexpression and purification of the gus was performed in beta-gluc ... | 2009 | 20075633 |
| purification and characterization of bacteriocin produced by strain of lactobacillus brevis mtcc 7539. | bacteriocin, an antimicrobial agent having potential for food biopreservation was purified from lactobacillus brevis (a safe food-grade bacteria isolated from vari kandal, a traditional fermented food of himachal pradesh by adopting a novel repeated washing method. its purity was confirmed by sds-page and native-page. the relative molecular mass of bacteriocin was 93.74 kd, while specific activity and recovery were 35.52 folds and 17.13%, respectively. it showed high thermal stability and was ac ... | 2009 | 19788067 |