Publications

study of the micro-organisms associated with the fermented bread (khamir) produced from sorghum in gizan region, saudi arabia.	traditional bread (khamir) was made from sorghum flour of two local varieties, bayadh and hamra. the bread was prepared by mixing the sorghum flour with water and spices (onion, garlic, lemon juice and fenugreek) in a 1:0.8 (w/w) ratio and fermented for 24 h at 30 degrees c. two other fermentations were carried out using an inoculum from the previous fermentation. the micro-organisms were isolated from different plates and identified using different characterization systems. both total bacterial ...	1999	10063620
the expression signals of the lactobacillus brevis slpa gene direct efficient heterologous protein production in lactic acid bacteria.	a cassette based on the expression signals of the lactobacillus brevis surface (s)-layer protein gene (slpa) was constructed. the low-copy-number vector pkth2095, derived from pgk12, was used as the cloning vector. the efficiency of slpa promoters in intracellular protein production was studied using three reporter genes, beta-glucuronidase (gusa), luciferase (luc) and aminopeptidase n (pepn) in three different lactic acid bacteria hosts: lactococcus lactis, lactobacillus plantarum and lactobaci ...	1999	10077822
tyrosine decarboxylase activity of lactobacillus brevis ioeb 9809 isolated from wine and l. brevis atcc 367.	tyramine, a frequent amine in wines, is produced from tyrosine by the tyrosine decarboxylase (tdc) activity of bacteria. the tyramine-producing strain lactobacillus brevis ioeb 9809 isolated from wine and the reference strain l. brevis atcc 367 were studied. at the optimum ph, 5.0, k(m) values of ioeb 9809 and atcc 367 crude extracts for l-tyrosine were 0.58 mm and 0.67 mm, and v(max) was higher for the wine strain (115 u) than the atcc 367 (66 u). tdc exhibited a preference for l-tyrosine over ...	1999	10547444
anticonvulsant activity of paeonimetabolin-i adducts obtained by incubation of paeoniflorin and thiol compounds with lactobacillus brevis.	seventeen thiopaeonimetabolin-i adducts were obtained as mixtures of diastereoisomers after incubation of paeoniflorin with lactobacillus brevis in the presence of various thiols. the anticonvulsant activity of the adducts was investigated in mice using the maximal subcutaneous pentylenetetrazol seizure test and sodium valproate (1.5 mmol/kg) as a positive control. thirteen adducts showed dose-dependent prolongation of latencies of clonic and tonic convulsions. maximal protection against convuls ...	1999	10375170
purification and partial amino acid sequence of plantaricin 1.25 alpha and 1.25 beta, two bacteriocins produced by lactobacillus plantarum tmw1.25.	two bacteriocins produced by lactobacillus plantarum tmw1.25 have been purified by a four-step purification procedure, including ammonium sulphate precipitation and cation-exchange chromatography followed by hydrophobic-interaction chromatography on octyl sepharose. the final purification was performed by repeated reversed-phase chromatography steps which yielded two bacteriocin fractions designated plantaricin 1.25 alpha and plantaricin 1.25 beta. the molecular masses of the peptides in these f ...	1999	10389253
molecular cloning, expression, and characterization of a thermostable glutamate racemase from a hyperthermophilic bacterium, aquifex pyrophilus.	a gene encoding glutamate racemase has been cloned from aquifex pyrophilus, a hyperthermophilic bacterium, and expressed in escherichia coli. the a. pyrophilus glutamate racemase is composed of 254 amino acids and shows high homology with glutamate racemase from escherichia coli, bacillus subtilis, or lactobacillus brevis. this racemase converts l- or d-glutamate to d- or l-glutamate, respectively, but not other amino acids such as alanine, aspartate, and glutamine. the cloned gene was expressed ...	1999	10484173
molecular identification of lactobacillus hilgardii and genetic relatedness with lactobacillus brevis.	conventional phenotypic methods lead to misidentification of the lactic acid bacteria lactobacillus hilgardii and lactobacillus brevis. random amplified polymorphic dna (rapd) and repetitive element pcr (rep-pcr) techniques were developed for a molecular study of these two species. the taxonomic relationships were confirmed by analysis of the ribosomal operon. amplified dna fragments were chosen to isolate l. hilgardii-specific probes. in addition to rapid molecular methods for identification of ...	1999	10425764
isolation, properties and behaviour of tyramine-producing lactic acid bacteria from wine.	wines containing high levels of biogenic amines were investigated for the presence of tyramine-producing strains. two different lactobacillus brevis (ioeb 9809 and ioeb 9901) able to produce the amine were isolated. none of the isolated strains identified as oenococcus oeni formed tyramine. in addition, other lact. brevis and lact. hilgardii strains from our collection (ioeb) and the american type culture collection (atcc) were strong tyramine producers. lactobacillus brevis ioeb 9809 and lact. ...	2000	10792516
gene cloning and expression and secretion of listeria monocytogenes bacteriophage-lytic enzymes in lactococcus lactis.	bacteriophage lysins (ply), or endolysins, are phage-encoded cell wall lytic enzymes which are synthesized late during virus multiplication and mediate the release of progeny virions. bacteriophages of the pathogen listeria monocytogenes encode endolysin enzymes which specifically hydrolyze the cross-linking peptide bridges in listeria peptidoglycan. ply118 is a 30.8-kda l-alanoyl-d-glutamate peptidase and ply511 (36.5 kda) acts as n-acetylmuramoyl-l-alanine amidase. in order to establish dairy ...	2000	10877791
application of a bioluminescence method for the beer industry: sensitivity of microstar-rmds for detecting beer-spoilage bacteria. rapid microbe detection system.	we set up the original operating conditions of the microstar-rapid microbe detection system (rmds) to suppress false positives, which have kept this system from practical. the detection limit of our system was between 6.3 x 10(-16) mol and 3.1 x 10(-16) mol in terms of the amount of atp, which is approximately equal to the atp content of one yeast cell or 50 lactic acid bacteria cells. the detection time and the detection count were compared between the rmd method and the conventional plate coun ...	2000	10879474
biochemical characterization of lactic acid bacteria isolated from spontaneous fermentation of 'almagro' eggplants.	a total of 149 strains of lactic acid bacteria isolated from the spontaneous fermentation of 'almagro' eggplants were characterized and identified. of the isolates, 148 were determined as belonging to the genus lactobacillus. a coccoid, gram-positive database-negative isolate was obtained in the early stages of fermentation. the lactobacillus strains were divided into six groups based on sugar fermentation patterns and other physiological and morphological characteristics, and were identified as ...	2000	10946834
crystallization and preliminary characterization of crystals of r-alcohol dehydrogenase from lactobacillus brevis.	the r-specific alcohol dehydrogenase (radh) from lactobacillus brevis is a valuable catalyst for the production of chiral alcohols that can be used as synthons in asymmetric syntheses. radh is a homotetramer with 222 symmetry and a molecular mass of 107 kda. the recombinant enzyme has been expressed in escherichia coli, purified to homogeneity and crystallized. the crystals belong to the orthorhombic space group i222, with unit-cell parameters a = 56.5, b = 85.1, c = 115.4 a, and diffract x-rays ...	2000	11092950
purification and characterization of novel antifungal compounds from the sourdough lactobacillus plantarum strain 21b.	sourdough lactic acid bacteria were selected for antifungal activity by a conidial germination assay. the 10-fold-concentrated culture filtrate of lactobacillus plantarum 21b grown in wheat flour hydrolysate almost completely inhibited eurotium repens ibt18000, eurotium rubrum ftdc3228, penicillium corylophilum ibt6978, penicillium roqueforti ibt18687, penicillium expansum idm/fs2, endomyces fibuliger ibt605 and idm3812, aspergillus niger ftdc3227 and idm1, aspergillus flavus ftdc3226, monilia s ...	2000	10966432
reduction of campylobacter jejuni in a simulated chicken digestive tract by lactobacilli cultures.	studies were conducted to investigate the impact of a selected lactobacilli mixed culture on campylobacter jejuni in simulated chicken digestive tract models. veronal buffer solutions corresponding to the ph of successive segments of the chicken digestive tract were prepared. the lactobacilli mixtures were prepared by mixing four fresh lactobacilli cultures, including lactobacillus acidophilus, lactobacillus fermenentum, lactobacillus crispatus, and lactobacillus brevis. the c. jejuni and lactob ...	2000	11079707
decarboxylation of substituted cinnamic acids by lactic acid bacteria isolated during malt whisky fermentation.	seven strains of lactobacillus isolated from malt whisky fermentations and representing lactobacillus brevis, l. crispatus, l. fermentum, l. hilgardii, l. paracasei, l. pentosus, and l. plantarum contained genes for hydroxycinnamic acid (p-coumaric acid) decarboxylase. with the exception of l. hilgardii, these bacteria decarboxylated p-coumaric acid and/or ferulic acid, with the production of 4-vinylphenol and/or 4-vinylguaiacol, respectively, although the relative activities on the two substrat ...	2000	11097909
purification and characterization of tyrosine decarboxylase of lactobacillus brevis ioeb 9809 isolated from wine.	tyrosine decarboxylase (ec 4.1.1.25) (tdc) from the wine lactobacillus brevis ioeb 9809 was purified by a rapid procedure involving anion exchange chromatography, ultrafiltration and hydrophobic interaction chromatography. the protein comprised two subunits of identical molecular mass (approximately 70000 da). enzyme activity was dependent on exogenously supplied pyridoxal 5'-phosphate and the enzyme was stable at 4 degrees c in the presence of the coenzyme. optimum ph for the pure enzyme was 5. ...	2001	11167003
hop resistance in the beer spoilage bacterium lactobacillus brevis is mediated by the atp-binding cassette multidrug transporter hora.	lactobacillus brevis is a major contaminant of spoiled beer. the organism can grow in beer in spite of the presence of antibacterial hop compounds that give the beer a bitter taste. the hop resistance in l. brevis is, at least in part, dependent on the expression of the hora gene. the deduced amino acid sequence of hora is 53% identical to that of lmra, an atp-binding cassette multidrug transporter in lactococcus lactis. to study the role of hora in hop resistance, hora was functionally expresse ...	2001	11514522
contribution of starter culture to the sensory characteristics of fermented almagro eggplants.	trial fermentations were performed using three experimental starter cultures with a view to selecting the most appropriate starter for use in the manufacture of almagro eggplants. the lactic acid bacterial strains used in the starters had previously been isolated from spontaneous fermentations. the combined action of the obligate heterofermentative species lactobacillus brevis and the facultative heterofermentative species l. plantarum yielded almagro eggplants with sensory properties preferred ...	2001	11518429
reaction kinetic interpretation of heat destruction influenced by environmental factors.	based on reaction kinetics and thermodynamic considerations a mathematical model was developed to describe the thermal death rate of microbes as a function of environmental factors such as temperature, water activity, ph and redox potential. the mathematical model is discussed and validated by fitting to data sets of heat destruction of lactobacillus brevis and lactobacillus plantarum. the mathematical analysis of the kinetic model and the parameters of the model fitting demonstrate its applicab ...	2001	11294350
genes involved in control of galactose uptake in lactobacillus brevis and reconstitution of the regulatory system in bacillus subtilis.	the heterofermentative lactic acid bacterium lactobacillus brevis transports galactose and the nonmetabolizable galactose analogue thiomethyl-beta-galactoside (tmg) by a permease-catalyzed sugar:h(+) symport mechanism. addition of glucose to l. brevis cells loaded with [(14)c]tmg promotes efflux and prevents accumulation of the galactoside, probably by converting the proton symporter into a uniporter. such a process manifests itself physiologically in phenomena termed inducer expulsion and exclu ...	2001	11325952
the effects of processing methods on the levels of lysine, methionine and the general acceptability of ogi processed using starter cultures.	batches of ogi were produced from maize flour slurry, dehulled maize grains and whole maize grains using saccharomyces cerevisiae and lactobacillus brevis as starter cultures. fermentation over time showed that dehulled maize grains inoculated with the starter cultures and processed whole maize grains requiring steeping led to higher acid production than fermentation with dry-milled maize slurry. ogi from dehulled maize grains inoculated with starter cultures showed the highest levels of lysine ...	2001	11205940
assessment of adhesion properties of novel probiotic strains to human intestinal mucus.	potential new probiotic strains lactobacillus brevis peli, l. reuteri ing1, l. rhamnosus vtt e-800 and l. rhamnosus lc-705 were assessed for their adhesion properties using the human intestinal mucus model. the effect on the adhesion of exposure to acid and pepsin and to milk were tested to simulate gastric and food processing conditions, and the effect of different growth media on adhesion was tested. the properties of the four strains were compared to the well-investigated probiotic l. rhamnos ...	2001	11252493
rapid identification of lactobacillus brevis using the polymerase chain reaction.	species-specific pcr was applied to identify lactobacillus brevis and the sensitivity and the specificity of the protocol were determined.	2001	11696100
identification of lactic acid bacteria constituting the predominating microflora in an acid-fermented condiment (tempoyak) popular in malaysia.	tempoyak is a traditional malaysian fermented condiment made from the pulp of the durian fruit (durio zibethinus). salt is sometime added to proceed fermentation at ambient temperature. in various samples obtained from night markets, lactic acid bacteria (lab) were the predominant microorganisms, ranging from log 8.4 to log 9.2 cfu g(-1). no other microorganisms were present to such a level. these samples contained reduced amount of saccharose, glucose and fructose but increased amount of d- and ...	2001	11205946
modulation of colonic barrier function by the composition of the commensal flora in the rat.	altered intestinal permeability is a key pathogenetic factor of idiopathic bowel inflammation. we investigated in the rat if changes in the composition of the bowel flora can alter colonic permeability.	2001	11247894
characterization of lactobacilli from scotch malt whisky distilleries and description of lactobacillus ferintoshensis sp. nov., a new species isolated from malt whisky fermentations.	sixty-four strains of lactobacillus were isolated from fermentation samples from 23 malt whisky distilleries located in the major whisky producing regions of scotland. the strains were assigned to 26 ribotype patterns. strains of some ribotype patterns were widely distributed and recovered from distilleries throughout scotland, while strains representing other ribotypes were particular to a specific region or even a certain distillery. repeated sampling of a single distillery over a 12 month per ...	2001	11283296
molecular cloning of a putative divalent-cation transporter gene as a new genetic marker for the identification of lactobacillus brevis strains capable of growing in beer.	random amplified polymorphic dna (rapd) pcr analysis of lactobacillus brevis isolates from breweries revealed that one of the random primers could distinguish beer-spoilage strains of l. brevis from nonspoilage strains. the 1.1-kb dna fragment amplified from all beer-spoilers included one open reading frame, termed hita (hop-inducible cation transporter), which encodes an integral membrane protein with 11 putative trans-membrane domains and a binding protein-dependent transport signature of a no ...	2001	11414327
new paeonilactone-a adducts formed by anaerobic incubation of paeoniflorin with lactobacillus brevis in the presence of arylthiols.	during the course of preparing anticonvulsant paeonimetabolin-i adducts, new paeonilactone-a adducts: 9-phenylthiopaeonilactone-a, 9-(o-tolylthio)paeonilactone-a, 9-(m-tolylthio)paeonilactone-a, 9-(p-tolylthio)-paeonilactone-a and 9-(2-naphthylthio)paeonilactone-a, were obtained along with expected paeonimetabolin-i adducts by anaerobic incubation of paeoniflorin from peony roots with lactobacillus brevis in the presence of the aromatic thiols, phenylthiol, o-tolylthiol, m-tolylthiol, p-tolylthi ...	2001	11456104
biocatalytic reduction of beta,delta-diketo esters: a highly stereoselective approach to all four stereoisomers of a chlorinated beta,delta-dihydroxy hexanoate.	a stereoselective chemoenzymatic synthesis of all four stereoisomers of tert-butyl 6-chloro-3,5-dihydroxy-hexanoate (6a) is presented. the key step of the sequence is a highly regio- and enantioselective single-site reduction of tert-butyl 6-chloro-3,5-dioxohexanoate (1a) by two enantiocomplementary biocatalysts. alcohol dehydrogenase from lactobacillus brevis (reclbadh) afforded a 72% yield of enantiopure tert-butyl (s)-6-chloro-5-hydroxy-3-oxohexanoate [(s)-2a]. the enantiomer (r)-2a was prepa ...	2001	11757647
crystal structure of maltose phosphorylase from lactobacillus brevis: unexpected evolutionary relationship with glucoamylases.	maltose phosphorylase (mp) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-d-glucose-1-phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. the enzyme is part of operons that are involved in maltose/malto-oligosaccharide metabolism. maltose phosphorylases have been classified in family 65 of the glycoside hydrolases. no structure is available for any member of this family.	2001	11587643
apoptotic effects of selected strains of lactic acid bacteria on a human t leukemia cell line are associated with bacterial arginine deiminase and/or sphingomyelinase activities.	the aim of the present work was, first, to analyze the apoptotic effect in vitro of sonicated preparations of selected strains of lactic acid bacteria on normal and tumor human lymphocytes. incubation with bacterial samples led to a relevant time-dependent apoptotic cell death of jurkat cells but not normal human peripheral blood lymphocytes. lactobacillus brevis (cd2) samples were more efficient in inducing apoptosis of jurkat cells than were samples of streptococcus thermophilus (s244). in an ...	2001	11962255
characterisation of lab in typical salento pecorino cheese.	twenty-nine strains of lactic acid bacteria isolated from the typical pecorino cheese of the salento area of italy, were identified and grouped according to their genetic similarity. a preliminary characterisation of the strains was conducted by means of morphological and biochemical analysis, but molecular approaches were necessary for the clear identification of the species. for the species detection, the amplification and sequencing of the 16s rdna gene was employed in addition, restriction a ...	2001	15954655
production of gamma-aminobutyric acid from alcohol distillery lees by lactobacillus brevis ifo-12005.	lactobacillus brevis ifo-12005 showed good growth in rice shochu distillery lees (kome shochu kasu). almost all of the free glutamic acid (10.50 mm) in shochu kasu was converted to gamma-amino-butyric acid (gaba) within 2 d of stationary culture at 30 degrees c. the amount of gaba in the kome shochu kasu medium finally reached 10.18 mm. after centrifugation of the broth culture, the supernatant fraction was treated with a flocculation agent to form a clear solution, then passed through a column ...	2002	16233172
identification by flagellum display of an epithelial cell- and fibronectin-binding function in the slpa surface protein of lactobacillus brevis.	depletion of the slpa protein from the bacterial surface greatly reduced the adhesion of lactobacillus brevis atcc 8287 to the human intestinal cell lines caco-2 and intestine 407, the endothelial cell line ea-hy926, and the urinary bladder cell line t24, as well as immobilized fibronectin. for functional analysis of the slpa surface protein, different regions of the slpa gene were expressed as internal in-frame fusions in the variable region of the flic(h7) gene of escherichia coli. the resulti ...	2002	12029053
development of a simple hplc method for determination of paeoniflorin-metabolizing activity of intestinal bacteria in rat feces.	a simple and reproducible hplc method for the determination of paeoniflorin (pf)-metabolizing activity of intestinal bacteria in rat feces was developed and validated. orally administered pf, a major active constituent of paeoniae radix, is metabolized into a bioactive compound, paeonimetabolin i (pm-i) by intestinal bacteria. direct determination of the pf-metabolizing rate into pm-i is hard to achieve by hplc due to the lack of intense chromophore in pm-i. however, when pf was incubated with l ...	2002	12237542
characterization of trehalose phosphorylase from bacillus stearothermophilus sk-1 and nucleotide sequence of the corresponding gene.	a bacterial trehalose phosphorylase (tpase; ec 2.4.1.64) was purified from the culture supernatant of bacillus stearothermophilus sk-1 to apparent homogeneity, and some properties were investigated. furthermore, a gene from sk-1 responsible for the tpase was cloned by southern hybridization with a degenerate oligonucleotide probe synthesized on the basis of the n-terminal sequence of the purified enzyme. the mr of the enzyme was estimated to be 150,000 by gel filtration and 83,000 by sds-page, s ...	2002	12400680
isolation of three new surface layer protein genes (slp) from lactobacillus brevis atcc 14869 and characterization of the change in their expression under aerated and anaerobic conditions.	two new surface layer (s-layer) proteins (slpb and slpd) were characterized, and three slp genes (slpb, slpc, and slpd) were isolated, sequenced, and studied for their expression in lactobacillus brevis neotype strain atcc 14869. under different growth conditions, l. brevis strain 14869 was found to form two colony types, smooth (s) and rough (r), and to express the s-layer proteins differently. under aerobic conditions r-colony type cells produced slpb and slpd proteins, whereas under anaerobic ...	2002	12446628
the biodiversity of lactic acid bacteria in greek traditional wheat sourdoughs is reflected in both composition and metabolite formation.	lactic acid bacteria (lab) were isolated from greek traditional wheat sourdoughs manufactured without the addition of baker's yeast. application of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total cell protein, randomly amplified polymorphic dna-pcr, dna-dna hybridization, and 16s ribosomal dna sequence analysis, in combination with physiological traits such as fructose fermentation and mannitol production, allowed us to classify the isolated bacteria into the species lactobaci ...	2002	12450829
proteolysis by sourdough lactic acid bacteria: effects on wheat flour protein fractions and gliadin peptides involved in human cereal intolerance.	sourdough lactic acid bacteria were preliminarily screened for proteolytic activity by using a digest of albumin and globulin polypeptides as a substrate. based on their hydrolysis profile patterns, lactobacillus alimentarius 15m, lactobacillus brevis 14g, lactobacillus sanfranciscensis 7a, and lactobacillus hilgardii 51b were selected and used in sourdough fermentation. a fractionated method of protein extraction and subsequent two-dimensional electrophoresis were used to estimate proteolysis i ...	2002	11823200
lactic acid bacteria and yeasts in kefir grains and kefir made from them.	in an investigation of the changes in the microflora along the pathway: kefir grains (a)-->kefir made from kefir grains (b)-->kefir made from kefir as inoculum (c), the following species of lactic acid bacteria (83-90%) of the microbial count in the grains) were identified: lactococcus lactis subsp. lactis, streptococcus thermophilus, lactobacillus delbrueckii subsp. bulgaricus, lactobacillus helveticus, lactobacillus casei subsp. pseudoplantarum and lactobacillus brevis. yeasts (10-17%) identif ...	2002	11938463
lactic acid production from wheat straw hemicellulose hydrolysate by lactobacillus pentosus and lactobacillus brevis.	lactic acid production by lactobacillus brevis and lactobacillus pentosus on a hemicellulose hydrolysate (hh) of wet-oxidized wheat straw was evaluated. the potential of 11-12 g/l fermentable sugars was released from the hh through either enzymatic or acidic pretreatment. fermentation of added xylose in untreated hh after wet-oxidation, showed no inhibition on the lactic acid production by either lb. pentosus or lb. brevis. lb. pentosus produced lactate corresponding to 88% of the theoretical ma ...	2002	11800488
biochemical characterization of hora-independent hop resistance mechanism in lactobacillus brevis.	we isolated a strain from hop-resistant lactobacillus hrevis abbc45, which had lost a plasmid (prh45) harboring a putative hop resistance gene, hora. the hop resistance level of this hora-deficient strain, named abbc45(c), was initially low but gradually induced by repeated growth in media containing progressively increasing levels of hop compounds. although the hop resistance level was substantially lower than that of the hop-adapted wild type strain, hop-adapted abbc45(c) (abbc45(cr)) was stil ...	2002	12051479
purification and partial gene sequence of the tyrosine decarboxylase of lactobacillus brevis ioeb 9809.	some lactic acid bacteria contain a tyrosine decarboxylase (tdc) which converts tyrosine to tyramine, a biogenic amine frequently encountered in fermented food and wine. purification and microsequencing of the tdc of lactobacillus brevis ioeb 9809 allowed us to determine a partial sequence of the tdc gene encoding 264 amino acids of the enzyme. analysis of this protein sequence revealed typical features of pyridoxal phosphate-dependent amino acid decarboxylases while not any known decarboxylase ...	2002	12052555
surface display of foreign epitopes on the lactobacillus brevis s-layer.	so far, the inability to establish viable lactobacillus surface layer (s-layer) null mutants has hampered the biotechnological applications of lactobacillus s-layers. in this study, we demonstrate the utilization of lactobacillus brevis s-layer subunits (slpa) for the surface display of foreign antigenic epitopes. with an inducible expression system, l. brevis strains producing chimeric s-layers were obtained after testing of four insertion sites in the slpa gene for poliovirus epitope vp1, that ...	2002	12450814
membrane-bound atpase contributes to hop resistance of lactobacillus brevis.	the activity of the membrane-bound h+-atpase of the beer spoilage bacterium lactobacillus brevis abbc45 increased upon adaptation to bacteriostatic hop compounds. the atpase activity was optimal around ph 5.6 and increased up to fourfold when l. brevis was exposed to 666 microm hop compounds. the extent of activation depended on the concentration of hop compounds and was maximal at the highest concentration tested. the atpase activity was strongly inhibited by n,n'-dicyclohexylcarbodiimide, a kn ...	2002	12406727
characterization and selection of vaginal lactobacillus strains for the preparation of vaginal tablets.	to characterize and select lactobacillus strains for properties that would make them a good alternative to the use of antibiotics to treat human vaginal infections.	2002	12392537
evolution of the lactic acid bacterial community during malt whisky fermentation: a polyphasic study.	the development of the lactic acid bacterial community in a commercial malt whisky fermentation occurred in three broad phases. initially, bacteria were inhibited by strong yeast growth. fluorescence microscopy and environmental scanning electron microscopy revealed, in this early stage, both cocci and rods that were at least partly derived from the wort and yeast but also stemmed from the distillery plant. denaturing gradient gel electrophoresis (dgge) of partial 16s rrna genes and sequence ana ...	2002	11772639
arginine catabolism by sourdough lactic acid bacteria: purification and characterization of the arginine deiminase pathway enzymes from lactobacillus sanfranciscensis cb1.	the cytoplasmic extracts of 70 strains of the most frequently isolated sourdough lactic acid bacteria were screened initially for arginine deiminase (adi), ornithine transcarbamoylase (otc), and carbamate kinase (ck) activities, which comprise the adi (or arginine dihydrolase) pathway. only obligately heterofermentative strains such as lactobacillus sanfranciscensis cb1; lactobacillus brevis am1, am8, and 10a; lactobacillus hilgardii 51b; and lactobacillus fructivorans dd3 and da106 showed all t ...	2002	12450844
isolation and biochemical characterization of a new nadh oxidase from lactobacillus brevis.	a new nadh oxidase, useful for the regeneration of nad+, was isolated and characterized from lactobacillus brevis. in crude extracts the activity was from 10-15 u mg(-1). after purification by four chromatographic steps, an activity of 116 u mg(-1) was obtained with 14% yield. highest activity was from ph 5.5-7 and at 40 degrees c. the enzyme requires dithiothreitol to prevent oxidative deactivation. the km value for nadh was 24 microm.	2003	12882306
evidence for chromosomal determination of fungicidal activity in strains of lactobacillus brevis and lactobacillus fermentum isolated from fermented foods.	the genetic basis of the fungicidal activity of strains of lactobacillus brevis and l. fermentum isolated from indigenous fermented foods was determined. a 5.5-kb plasmid was isolated from l. brevis while l. fermentum was found to harbor no plasmid. plasmid curing indicated no correlation between the plasmid and the fungicidal activity of the lactobacillus species. the fungicidal activity of the isolated organisms can be supposed to be mediated by the chromosome. no antibiotic resistance genetic ...	2003	12744077
use of starter cultures of lactic acid bacteria and yeasts in the preparation of togwa, a tanzanian fermented food.	starter cultures of lactic acid bacteria (lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum and pediococcus pentosaceus) and yeasts (candida pelliculosa, candida tropicalis, issatchenkia orientalis and saccharomyes cerevisiae) isolated from native togwa were tested singly or in combination for their ability to ferment maize-sorghum gruel to produce togwa. all species of bacteria showed an ability to ferment the gruel as judged by lowering the ph fr ...	2003	12745235
phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis.	a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ...	2003	12514026
acetic acid increases stability of silage under aerobic conditions.	the effects of various compounds on the aerobic stability of silages were evaluated. it has been observed that inoculation of whole-crop maize with homofermentative lactic acid bacteria leads to silages which have low stability against aerobic deterioration, while inoculation with heterofermentative lactic acid bacteria, such as lactobacillus brevis or lactobacillus buchneri, increases stability. acetic acid has been proven to be the sole substance responsible for the increased aerobic stability ...	2003	12514042
the crystal structure of r-specific alcohol dehydrogenase from lactobacillus brevis suggests the structural basis of its metal dependency.	the crystal structure of the apo-form of an r-specific alcohol dehydrogenase from lactobacillus brevis (lb-radh) was solved and refined to 1.8a resolution. lb-radh is a member of the short-chain dehydrogenase/reductase (sdr) enyzme superfamily. it is a homotetramer with 251 amino acid residues per subunit and uses nadp(h) as co-enzyme. nadph and the substrate acetophenone were modelled into the active site. the enantiospecificity of the enzyme can be explained on the basis of the resulting hypot ...	2003	12628239
isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of escherichia coli o157:h7 in cattle.	lactic acid bacteria (lab) were selected on the basis of characteristics indicating that they would be good candidates for a competitive exclusion product (cep) that would inhibit escherichia coli o157:h7 in the intestinal tract of live cattle. fecal samples from cattle that were culture negative for e. coli o157:h7 were collected. lab were isolated from cattle feces by repeated plating on deman rogosa sharpe agar and lactobacillus selection agar. six hundred eighty-six pure colonies were isolat ...	2003	12636285
microbiological and fermentation characteristics of togwa, a tanzanian fermented food.	selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ...	2003	12423921
the tyrosine decarboxylase operon of lactobacillus brevis ioeb 9809: characterization and conservation in tyramine-producing bacteria.	bacterial genes of tyrosine decarboxylases were recently identified. here we continued the sequencing of the tyrosine decarboxylase locus of lactobacillus brevis ioeb 9809 and determined a total of 7979 bp. the sequence contained four complete genes encoding a tyrosyl-trna synthetase, the tyrosine decarboxylase, a probable tyrosine permease and a na+/h+ antiporter. rapid amplification of cdna ends (race) was employed to determine the 5'-end of mrnas containing the tyrosine decarboxylase gene. it ...	2003	14659544
cloning, sequence analysis and expression of the f1f0-atpase beta-subunit from wine lactic acid bacteria.	the nucleotide sequences of the genes encoding the f1f0-atpase beta-subunit from oenococcus oeni, leuconostoc mesenteroides subsp. mesenteroides, pediococcus damnosus, pediococcus parvulus, lactobacillus brevis and lactobacillus hilgardii were determined. their deduced amino acid sequences showed homology values of 79-98%. data from the alignment and atpase tree indicated that o. oeni and l. mesenteroides subsp. mesenteroides formed a group well-separated from p. damnosus and p. parvulus and fro ...	2003	14529177
16s-ardra, a tool for identification of lactic acid bacteria isolated from grape must and wine.	lactic acid bacteria (lab) are found in a great variety of habitats, including grape must and wines. there is a close relationship between the species of lab which develop during fermentation and the eventual quality of the wine. for these reasons analytical techniques allowing fast and reliable identification of wine lab are needed. in this work a simple and accurate protocol for identifying species of lab isolated from grape must and wine is presented. this protocol is based on the amplificati ...	2003	14529184
novel leuconostoc citreum starter culture system for the fermentation of kimchi, a fermented cabbage product.	to determine the dominant microorganisms involved in kimchi fermentation and to examine their effect on kimchi fermentation, we randomly isolated and characterized 120 lactic acid bacteria from kimchi during a 5-day fermentation at 15 degrees c. leuconostoc citreum was dominant during the early and mid-phases of kimchi fermentation whereas lactobacillus sake/lactobacillus curvatus or lactobacillus brevis were found during later stages. eighty-two out of 120 isolates (68%) were identified as leuc ...	2003	14574101
antimicrobial interactions of microbial species involved in the fermentation of cassava dough into agbelima with particular reference to the inhibitory effect of lactic acid bacteria on enteric pathogens.	lactic acid bacteria, bacillus species and yeasts are involved in the fermentation of cassava dough into agbelima. microbial interactions within and between these groups of microorganisms were investigated in addition to the survival of five enteric pathogens inoculated into agbelima under various conditions. nine out of 10 cultures of lactic acid bacteria isolated at the end of agbelima fermentation showed inhibitory effect against 10 cultures of lactic acid bacteria isolated at the start of fe ...	2003	14580972
construction and use of a computerized dna fingerprint database for lactic acid bacteria from silage.	efficient selection of new silage inoculant strains from a collection of over 10,000 isolates of lactic acid bacteria (lab) requires excellent strain discrimination. toward that end, we constructed a gelcompar ii database of dna fingerprint patterns of ethidium bromide-stained ecori fragments of total lab dna separated by conventional agarose gel electrophoresis. we found that the total dna patterns were strain-specific; 56/60 american type culture collection strains of 33 species of lab could b ...	2003	14607400
the potential of dairy lactic acid bacteria to metabolise amino acids via non-transaminating reactions and endogenous transamination.	the metabolism of amino acids by 22 starter and 49 non-starter lactic acid bacteria (lab) was studied in a system consisting of amino acids and non-growing cells without added amino acceptors such as alpha-ketoglutarate. there were significant inter- and intra-species differences in the metabolism of amino acids. some amino acids such as alanine, arginine, aspartate, serine and branched-chain amino acids (leucine, isoleucine and valine) were utilised, whereas other amino acids such as glycine, o ...	2003	12915037
probiotic and milk technological properties of lactobacillus brevis.	two lactobacillus brevis strains atcc 8287 and atcc 14869(t), were evaluated for their applicability as putative probiotics in dairy products. the strains expressed good in vitro adherence to human caco-2 and intestine 407 cells and tolerated well low ph, bile acids and pancreatic fluid under in vitro conditions. in antimicrobial activity assays, strain atcc 8287 showed inhibitory properties toward selected potential harmful microorganisms, particularly against bacillus cereus. both l. brevis st ...	2003	12672593
enumeration of bacterial cell numbers by amplified firefly bioluminescence without cultivation.	we recently developed a novel bioluminescent enzymatic cycling assay for atp and amp with the concomitant use of firefly luciferase and pyruvate orthophosphate dikinase (ppdk), where amp and pyrophosphate produced from atp by firefly luciferase were converted back into atp by ppdk. background luminescence derived from contaminating atp and amp in the reagent was reduced using adenosine phosphate deaminase which degrades atp, adp, and amp, resulting in constant and highly amplified bioluminescenc ...	2003	12479834
surface display of the receptor-binding region of the lactobacillus brevis s-layer protein in lactococcus lactis provides nonadhesive lactococci with the ability to adhere to intestinal epithelial cells.	lactobacillus brevis is a promising lactic acid bacterium for use as a probiotic dietary adjunct and a vaccine vector. the n-terminal region of the s-layer protein (slpa) of l. brevis atcc 8287 was recently shown to mediate adhesion to various human cell lines in vitro. in this study, a surface display cassette was constructed on the basis of this slpa receptor-binding domain, a proteinase spacer, and an autolysin anchor. the cassette was expressed under control of the nisa promoter in lactococc ...	2003	12676705
proteolytic activities in togwa, a tanzanian fermented food.	proteolytic activities were investigated in sorghum-based togwa prepared by natural fermentation and using starter cultures previously isolated from the native product, i.e., lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum, pediococcus pentosaceus, and issatchenkia orientalis in coculture with either l. brevis or l. plantarum. both proteinase and aminopeptidase activities were substantially higher in naturally fermented togwa than in those with s ...	2003	12781948
food additive lactic acid production by immobilized cells of lactobacillus brevis on delignified cellulosic material.	improvements in yield and productivity in lactic acid fermentation by lactobaccilus brevis cells immobilized on delignified cellulosic (dc) material are reported. the system proved to be more efficient in comparison with the work reported by other workers. yields of 80 and 100% conversion using glucose were obtained at 30 degrees c in 1 day of fermentation time. lactic acid fermentation using whey as substrate was obtained at 30 degrees c in 1-1.5 days, resulting in 70% yield, whereas the remain ...	2003	12926871
beer spoilage bacteria and hop resistance.	for brewing industry, beer spoilage bacteria have been problematic for centuries. they include some lactic acid bacteria such as lactobacillus brevis, lactobacillus lindneri and pediococcus damnosus, and some gram-negative bacteria such as pectinatus cerevisiiphilus, pectinatus frisingensis and megasphaera cerevisiae. they can spoil beer by turbidity, acidity and the production of unfavorable smell such as diacetyl or hydrogen sulfide. for the microbiological control, many advanced biotechnologi ...	2003	14623377
fermented vegetable juices obtained from celery, parsley and tomato.	application of starter cultures in fermented food industry results in obtaining high quality products, standardized sensory properties and allows to shorten a time of production. employment of appropriate starter cultures indices the possibility of develop a new assortment of fermented products which can not be obtain in traditional way. the aim of study was to produce fermented vegetable juices using starter cultures with different quantitative and qualitative composition. parsley, celery and t ...	2003	24757797
identification of lactic acid bacteria isolated from south african brandy base wines.	in brandy base wines, no sulphur dioxide is used and it therefore is ideal for the proliferation of lactic acid bacteria. as part of an extensive taxonomic survey within the ecological framework of south african vineyards and wineries, and the influence of naturally occurring lactic acid bacteria on the quality of wine and brandy, a total of 54 strains were isolated from grape juice and at different stages of brandy base wine production. the strains were identified using numerical analysis of to ...	2004	14967557
optimization of enzymatic gas-phase reactions by increasing the long-term stability of the catalyst.	enzymatic gas-phase reactions are usually performed in continuous reactors, and thus very stable and active catalysts are required to perform such transformations on cost-effective levels. the present work is concerned with the reduction of gaseous acetophenone to enantiomerically pure (r)-1-phenylethanol catalyzed by solid alcohol dehydrogenase from lactobacillus brevis (lbadh), immobilized onto glass beads. initially, the catalyst preparation displayed a half-life of 1 day under reaction condi ...	2004	15176907
comparative analysis of conserved genetic markers and adjacent dna regions identified in beer-spoilage lactic acid bacteria.	to conduct an inter-species comparative study on the nucleotide sequences of the conserved dna regions surrounding orf5, a genetic marker for differentiating beer-spoilage lactic acid bacteria.	2004	15287868
cloning and sequencing of kojibiose phosphorylase gene from thermoanaerobacter brockii atcc35047.	a gene encoding kojibiose phosphorylase was cloned from thermoanaerobacter brockii atcc35047. the kojp gene encodes a polypeptide of 775 amino acid residues. the deduced amino acid sequence was homologous to those of trehalose phosphorylase from t. brockii and maltose phosphorylases from bacillus sp. and lactobacillus brevis with 35%, 29% and 28% identities, respectively. kojibiose phosphorylase was efficiently overexpressed in escherichia coli jm109. the dna sequence of 3956 bp analyzed in this ...	2004	16233673
[studies on the biotransformation of escin ia by human intestinal bacteria and the anti-tumor activities of desacylescin i].	to study biotransformation of escin ia by the crude enzymes of human intestinal bacteria and lactobacillus brevis, determine the structures of biotransformation products and assay the inhibitory effect of desacylescin i on the tumor cell growth.	2004	14970884
a pcr assay for detection of acetic acid-tolerant lactic acid bacteria in acidic food products.	a pcr assay for the detection of acetic acid-tolerant lactic acid bacteria in the genera of lactobacillus and pediococcus was developed in this study. primers targeting the bacterial 16s rrna gene were newly designed and used in this pcr assay. to determine the specificity of the assay, 56 different bacterial strains (of 33 genera), 2 fungi, 3 animals, and 4 plants were tested. results were positive for most tested bacterial members of 16s rrna gene-based phylogenetic groups (classified in the l ...	2004	15035383
the influence of lactobacillus brevis on ornithine decarboxylase activity and polyamine profiles in helicobacter pylori-infected gastric mucosa.	functional probiotics may prevent helicobacter pylori infection, and some evidence suggests that they also possess antitumor properties. lactobacillus brevis (cd2) is a functional lactobacillus strain with peculiar biochemical features, essentially related to the activity of arginine deiminase. this enzyme catalyzes the catabolism of arginine and affects the biosynthesis of polyamines (putrescine, spermidine, and spermine). polyamines are polycations found in high concentrations in both normal a ...	2004	15068419
use of an ionic liquid in a two-phase system to improve an alcohol dehydrogenase catalysed reduction.	due to favourable partition coefficients the highly enantioselective reduction of 2-octanone, catalysed by an alcohol dehydrogenase from lactobacillus brevis, is faster in a biphasic system containing buffer and the ionic liquid [bmim][(cf(3)so(2))(2)n] compared to the reduction in a biphasic system containing buffer and methyl tert-butyl ether.	2004	15116196
characterisation of the microbiota of rice sourdoughs and description of lactobacillus spicheri sp. nov.	the microbiota of two industrially processed rice sourdoughs was characterised by bacteriological culture in combination with pcr-denaturing gradient gel electrophoresis (dgge) and 16s/28s rdna sequence analysis. rice sourdough i was continuously propagated for several years by back-slopping every week, whereas sourdough ii was processed by using a commercial starter culture and back-slopping daily for three days. in rice sourdough ii candida krusei and saccharomyces cerevisiae as well as lactob ...	2004	15046303
production of phenyllactic acid by lactic acid bacteria: an approach to the selection of strains contributing to food quality and preservation.	the ability of lactic acid bacteria (lab) to produce phenyllactic (pla) and 4-hydroxy-phenyllactic (oh-pla) acids, metabolites involved in food quality and preservation, has been evaluated by hplc analysis in 29 lab strains belonging to 12 species widely used in the production of fermented foods. metabolite production was demonstrated for all strains of the species lactobacillus plantarum, lactobacillus alimentarius, lactobacillus rhamnosus, lactobacillus sanfranciscensis, lactobacillus hilgardi ...	2004	15063498
lactobacillus casei, dominant species in naturally fermented sicilian green olives.	this study investigated the phenotypic and genotypic characteristics of lactic acid bacteria in naturally fermented green olives, collected from different areas of sicily. both classical biochemical tests and pcr/restriction fragments length polymorphism (rflp) of 16s rdna were used to characterize the isolates. the identity of the isolates was obtained by the partial sequencing analysis of the 16s rdna. the biomerieux software assigned the 13 heterofermentative strains to the lactobacillus brev ...	2004	14672826
genetic characterization of non-spoilage variant isolated from beer-spoilage lactobacillus brevis abbc45.	to characterize the non-spoilage variant obtained from beer-spoilage lactobacillus brevis abbc45c and to identify a potential genetic marker capable of discriminating beer-spoilage l. brevis strains from non-spoilers.	2004	15078510
souring and breakdown of cyanogenic glucosides during the processing of cassava into akyeke.	the population and composition of the lactic acid bacteria microbiota as well as the content of cyanogenic glucosides occurring at various stages of fermentation and subsequent processing of cassava roots into akyeke, a steamed sour cassava meal, were investigated. the number of lactic acid bacteria and percentage titratable acidity increased during 5 days of fermentation, but decreases were observed in the subsequent operations of 'washing' the dough with water followed by partial drying and st ...	2004	15135588
detection of resistance of lactic acid bacteria to a mixture of the hop analogue compounds tetrahydroiso-alpha-acids by noninvasive measurement of intracellular ph.	to examine the resistance of beer isolates of lactic acid bacteria (lab) towards a mixture of tetrahydroiso-alpha-acids (tetra) by growth experiments as well as by measurement of intracellular ph.	2004	15139925
synthesis and characterization of bioconjugates of s-layer proteins.	the self-assembling proteins that form crystalline surface layers (s-layers) on many microbial species have found numerous applications due to their nanostructured nature. to devise a new method to construct surface displays that exploit s-layer self-assembly activity and nanostructural properties, we have constructed polymer bioconjugates of s-layer proteins. the conjugates formed are similar in function to the monomer alkanethiols that form self-assembled monolayers (sams) on gold surfaces. ho ...	2004	15264854
[microbiological study of dehydrated garlic (allium sativum l.) and onion (allium cepa l.)].	a microbiological study during the process and the storage of garlic (allium sativum l.) and onion (allium cepa l.) dehydrated, with the additional barriers of blanching or brine immersion, was made. in all raw materials the average counts of aerobic mesophilic bacteria expressed in cfu/g ranged from 1.2 x 10(2) to 1.6 x 10(3), molds and yeasts from 60 to 1.6 x 10(3), lactobacillus spp. and leuconostoc mesenteroides subsp. mesenteroides between 10 and 50. microorganisms identified were penicilli ...	2004	15559197
phytate degradation by lactic acid bacteria and yeasts during the wholemeal dough fermentation: a 31p nmr study.	myo-inositol hexaphosphate (ip6) is the main source of phosphorus in cereal grains, and therefore, in bakery products. different microorganisms such as yeasts and lactic acid bacteria have phytase enzymes able to hydrolyze ip6 during the wholemeal breadmaking. in this paper, the phytase activity of lactobacillus plantarum, lactobacillus brevis, lactobacillus curvatus, and saccharomyces cerevisiae strains, isolated from southern italian sourdoughs, is assayed using the (31)p nmr technique. the so ...	2004	15453704
screening of lactic acid bacteria from fermented vegetables by carbohydrate profiling and pcr-elisa.	the aim of this study was to identify potential souring agents, isolated from fermented plant material, by api 50 chl assay and a molecular method based on polymerase chain reaction and colorimetric hybridization (pcr-elisa).	2004	15482435
isolation of a lactic acid bacterium and yeast consortium from a fermented material of ulva spp. (chlorophyta).	microbiota in a fermented culture of ulva spp. was examined with the objective to characterize the type of fermentation and to obtain starter microbes for performing seaweed fermentation.	2004	15546421
polyphasic study of wine lactobacillus strains: taxonomic implications.	one hundred and seventy-eight lactobacilli isolated from wine were characterized by a polyphasic approach. strains were phenotypically identified at genus and species level by classical tests including the analysis of cell morphology, homo/heterofermentative character, sugar fermentation patterns, growth at different temperatures and the optical nature of the isomer of lactic acid produced from glucose. molecular techniques such as random amplification of polymorphic dna (rapd), amplified 16s rd ...	2005	15653876
bacterial counts of intestinal lactobacillus species in infants with colic.	intestinal colonization by lactobacilli is suggested to be a prerequisite to normal mucosal immune functions. an inadequate level of lactobacilli may be involved in appearance of allergic disease of which, infantile colic, is often considered an early clinical manifestation. the aim of the study is to evaluate intestinal lactobacilli in breast-fed infants with infantile colic and healthy infants. fifty-six breast-fed infants, aged 15-60 days were enrolled in the study and divided into two groups ...	2005	15693915
antibiotic susceptibility of lactobacillus and bifidobacterium species from the human gastrointestinal tract.	one hundred and twenty-two strains of bifidobacterium and lactobacillus species have been tested against 12 antibiotics and two antibiotic mixtures by a commercial system (sensititre anaero3; treck diagnostic systems). the upper limits of some minimum inhibitory concentrations (mics) were completed on mrs agar plates by the nccls procedure. all strains were sensitive to chloramphenicol and imipenem and most of the strains were resistant to metronidazole. bifidobacteria isolates were susceptible ...	2005	15902467
random amplified polymorphic dna-pcr based cloning of markers to identify the beer-spoilage strains of lactobacillus brevis, pediococcus damnosus, lactobacillus collinoides and lactobacillus coryniformis.	beer-spoilage ability of lactic acid bacteria such as lactobacillus brevis is a strain-dependent phenomenon in which the mechanism has not yet been completely clarified. in order to systematically identify genes that contribute to beer-spoilage, large-scale random amplified polymorphic dna (rapd)-based cloning methods was carried out.	2005	15836491
rapid identification of dairy lactic acid bacteria by m13-generated, rapd-pcr fingerprint databases.	about a thousand lactic acid bacteria (lab) isolated from dairy products, especially cheeses, were identified and typed by species-specific pcr and rapd-pcr, respectively. rapd-pcr profiles, which were obtained by using the m13 sequence as a primer, allowed us to implement a large database of different fingerprints, which were analysed by bionumerics software. cluster analysis of the combined rapd-pcr fingerprinting profiles enabled us to implement a library, which is a collection of library uni ...	2005	15893395
cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlk from lactobacillus brevis.	the commercial production of mannitol involves high-pressure hydrogenation of fructose using a nickel catalyst, a costly process. mannitol can be produced through fermentation by microorganisms. currently, a few lactobacillus strains are used to develop an efficient process for mannitol bioproduction; most of the strains produce mannitol from fructose with other products. an approach toward improving this process would be to genetically engineer lactobacillus strains to increase fructose-to-mann ...	2005	15917616
hepatoprotective effect of lactic acid bacteria, inhibitors of beta-glucuronidase production against intestinal microflora.	the hepatoprotective activity of lactic acid bacteria (lactobacillus brevis hy7401, lactobacillus acidophilus csg and bifidobacterium longum hy8001), which inhibited beta-glucuronidase productivity of intestinal microflora, on t-bhp- or ccl4-induced hepatotoxicity of mice were evaluated. these oral administration of lactic acid bacteria lowered beta-glucuronidase production of intestinal microflora as well as escherichia coli hgu-3. when lactic acid bacteria at a dose of 0.5 or 2 g (wet weight)/ ...	2005	15832821
production of xylitol from d-xylose by recombinant lactococcus lactis.	the d-xylose reductase from pichia stipitis cbs 5773 and the xylose transporter from lactobacillus brevis atcc 8287 were expressed in active form in lactococcus lactis nz9800. xylitol production was investigated using non-growing recombinant cells in high cell-density under microaerobic conditions in the presence of xylose and glucose. besides xylose, the recombinant strain with xylose reductase activity reduced l-arabinose and d-ribose in significant extent to the corresponding pentitols. the r ...	2005	15916828
enantioselective reduction of carbonyl compounds by whole-cell biotransformation, combining a formate dehydrogenase and a (r)-specific alcohol dehydrogenase.	a whole-cell biotransformation system for the reduction of prochiral carbonyl compounds, such as methyl acetoacetate, to chiral hydroxy acid derivatives [methyl (r)-3-hydroxy butanoate] was developed in escherichia coli by construction of a recombinant oxidation/reduction cycle. alcohol dehydrogenase from lactobacillus brevis catalyzes a highly regioselective and enantioselective reduction of several ketones or keto acid derivatives to chiral alcohols or hydroxy acid esters. the adh gene encodin ...	2005	15549291
gene cloning, functional expression and secretion of the s-layer protein sgse from geobacillus stearothermophilus nrs 2004/3a in lactococcus lactis.	the ~93-kda surface layer protein sgse of geobacillus stearothermophilus nrs 2004/3a forms a regular crystalline array providing a nanopatterned matrix for the future display of biologically relevant molecules. lactococcus lactis nz9000 was established as a safe expression host for the controlled targeted production of sgse based on the broad host-range plasmid pnz124sph, into which the nisa promoter was introduced. sgse devoid of its signal peptide-encoding sequence was cloned into the new vect ...	2005	15675069
enzyme-catalyzed regio- and enantioselective ketone reductions.	in this chapter, examples are given of the application of highly reactive prochiral ketones as substrates for enzymatic reductions. 3,5-dioxocarboxylates are polyketide-like compounds that can be used to synthesize all of the possible stereoisomers of the corresponding 1,3-diols by means of regio- and enantioselective enzymatic reduction. the results obtained from an investigation into the usefulness of the resulting hydroxyl ketones and 1,3-diols in organic synthesis led to the development of n ...	2005	15791940