| lactase activity of microorganisms. | sixty-two strains of yeasts, molds and bacteria were screened for lactase (beta-d-galactosidase) activity. strains exhibiting the enzyme activity were evaluated for cell yield as well as enzyme units available per litre of the medium, per g cell dry weight and per mg protein of their cell-free extracts. the molds exhibited lowest enzyme activity but highest cell yields, bacteria produced lowest cell yield and maximum enzyme activity. cultures exhibiting very high activity among yeasts were sacch ... | 1978 | 97187 |
| influence of type of antibiotic and length of antibiotic feeding period on performance and persistence of antibiotic resistant enteric bacteria in growing-finishing swine. | | 1978 | 97254 |
| [effect of sodium fluoride on samples of oral flora. clinical application]. | | 1977 | 102105 |
| dihydrofolate reductase from amethopterin-resistant lactobacillus casei. sequences of the cyanogen bromide peptides and complete sequences of the enzyme. | the complete amino acid sequence of dihydrofolate reductase from an amethopterin-resistant strain of lactobacillus casei has been determined by sequence analysis of peptides produced by cleavage with cyanogen bromide, trypsin, staphylococcal protease, and myxobacter protease. comparison of this sequence with those of reductases from other bacterial sources shows that the enzymes are homologous. the lactobacillus casei reductase sequences shows a 29% sequence identity with that of the escherichia ... | 1978 | 98527 |
| vegetarian vs. normal as casual agents in tooth decay. | | 1977 | 96194 |
| 8-hydroxyquinolines with the potential for long-term anticaries activity: design, synthesis, and in vitro evaluation. | 8-hydroxyquinolyl benzoate and 8-hydroxyquinolyl para-(fluorosulfonyl)benzoate have been synthesized and evaluated in vitro as antimicrobial and antiplaque agents. both compounds inhibited the growth of cultures of the following genera: streptococcus, staphylococcus, actinomyces, lactobacillus, veillonella, and candida. minimum inhibitory concentrations ranged from 0.98 to 62 microgram/ml. extracted human teeth pretreated with 1% solutions of either compound and rinsed with distilled water exhib ... | 1978 | 98543 |
| oligosaccharides and polysaccharides specifically utilizable by bifidobacteria. | | 1978 | 103638 |
| folate analogues altered in the c9-n10 bridge region: n10-toxylisohomofolic acid and n10-toxylisohomoaminopterin. | | 1978 | 97383 |
| antigenic analyses of lactobacillus fermenti. | antigenic analyses of lactobacillus fermenti were carried out by double immunodiffusion in agar using extracts prepared with cold trichloroacetic acid (tca) or hot dilute hydrochloric acid (hcl). a common antigen of l. fermenti, designated as antigen f by the author, was extracted from whole cells with dilute hcl, but not with tca. the antigen f was also observed in lactobacillus casei. in addition, all strains isolated from human saliva contained antigen 6 in their cell walls, while the antigen ... | 1975 | 50468 |
| the primary structure of lactobacillus casei thymidylate synthetase. iii. the use of 2-(2-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine and limited tryptic peptides to establish the complete amino acid sequence of the enzyme. | | 1979 | 105005 |
| glycerol teichoic acid as an antigenic determinant in a gram-negative bacterium butyrivibrio fibrisolvens. | an antigenic determinant isolated from a strain of the gram-negative bacterium butyrivibrio fibrisolvens reacted with specific antisera to the polyglycerophosphate backbone of membrane teichoic acids of lactobacilli. it gave a reaction of identity with membrane glycerol lipoteichoic acid and glycerol teichoic acid preparations from lactobacilli, and with phenol extracts of other gram-positive bacteria. the antigen-antibody reactions was strongly inhibited by glycerol-phosphoryl-glycerol-phosphor ... | 1975 | 50403 |
| cotrimoxazole and folate metabolism. | cotrimoxazole 4 tablets daily (1 tablet = trimethoprim 80 mg and sulphamethoxzole 400 mg) was given for a period of six to fourteen days to 13 inpatients, and serum-folate levels were measured before and one day after the course of treatment. the results were compared with those from 8 patients not receiving antibacterial therapy, tested on admission and one week later. two assay techniques were used, one employing lactobacillus casei and the other 125i-labelled folate isotope dilution. the micr ... | 1976 | 60570 |
| peptide hydrolases of lactobacillus casei: isolation and general properties of various peptidase activities. | discovery of an endopeptidase by gel chromatography and separation of 3 exopeptidases (a dipeptidase, an aminopeptidase and a specific carboxypeptidase) from lactobacillus casei ncdo 151 by affinity chromatography is described. the 3 exopeptidases were strongly inhibited by the metal chelators edta and 1,10-phenanthroline but were reactivated with co2+ and mn2+. the ph optima for aminopeptidase, dipeptidase and carboxypeptidase activities were 6.5, 7.6 and 7.2, respectively. maximum activity was ... | 1978 | 101561 |
| detection and localization of peptide hydrolases in lactobacillus casei. | | 1978 | 101562 |
| [volatile fatty acids in gastrointestinal diseases in young infants]. | | 1978 | 101958 |
| adhesion of lactobacilli to the chicken crop epithelium. | | 1975 | 50457 |
| grouping and cross-reacting antigens of oral lactic acid bacteria. | the grouping antigens from five of the seven serological groups of lactobacilli have been defined with respect to the carbohydrate units responsible for serological specificity. when the antigens are polysaccharides, specificity is more absolute than with the teichoic acids where cross-reactions may occur because of the same or similar carbohydrate substituents, or more particularly because of the common "backbone" structure of the glycerol teichoic acids. this glycerol phosphate "backbone" acco ... | 1976 | 54372 |
| [lactic acid bacteria and streptococci of the human stomach normally and in pathological processes]. | lactic acid bacteria were either absent or present in negligent quantities in the stomach of apparently healthy man. but in case of various pathological processes in the stomach and duodenum lactic acid bacteria accumulated (up to a million cells per 1 ml of gastric contents). under these conditions coccal forms sharply prevailed over the bacillary ones. in patients with chronic gastritis and peptic ulcer of the stomach the most frequent were streptococcus faecalis and its variants; in oncologic ... | 1979 | 107685 |
| results with commercial radioassay kits compared with microbiological assay of folate in serum and whole-blood. | we compared results with three commercial folate radioassay kits [bio-rad, new england nuclear (nen), and ria products] with those by microbiological assay for more than 200 samples of human serum and whole blood. all but one kit (nen) compared favorably with the microbiological assay for serum samples, although there were notable diagnostic discrepancies. two kits (nen and bio-rad) were tested on whole-blood samples; both yielded values significantly higher than those by microbiological assay. ... | 1978 | 102464 |
| comparison of radioassay and microbiological assay for serum folate, with clinical assessment of discrepant results. | folate assays by use of radiolabeled folate provide obvious practical advantages over the standard microbiological assay, but remain incompletely tested. we therefore compared results for 415 sera with a kit involving 3h-labeled folate and the lactobacillus casei microbiological method. we examined the patients' data when there were discrepancies between the two methods. although the correlation overall was satisfactory, results were discrepant in 25% of cases. in 74% of the latter, the radioass ... | 1978 | 102465 |
| anaerobic fecal bacteria of the baboon. | the predominant bacterial genera of baboon feces were enumerated and identified by established procedures. the predominant genera isolated were lactobacillus, eubacterium, streptococcus, and bacteroides. | 1978 | 103499 |
| 5-nitro-2'-deoxyuridine 5'-monophosphate is a potent irreversible inhibitor of lactobacillus caesi thymidylate synthetase. | | 1978 | 103545 |
| separation and identification of pteroylpolyglutamates by polyacrylamide gel chromatography. | | 1979 | 109018 |
| 5-nitro-2'-deoxyuridylate: a mechanism-based inhibitor of thymidylate synthetase. | | 1978 | 102324 |
| control of intestinal flora in animals and humans: implications for toxicology and health. | the importance of the intestinal flora in disease management is discussed, and apparent relationships noted in experimental and clinical reports are reviewed. it is suggested that investigation of the influence of the intestinal flora upon body organs and systems would productively lay the groundwork for abetter understanding of certain synergistic mechanisms. | 1978 | 102713 |
| increased synthesis of ribonucleotide reductase after deoxyribonucleic acid inhibition in various species of bacteria. | the specific activity of ribonucleotide reductase was found to increase significantly after deoxyribonucleic acid inhibition in seven species of bacteria investigated. this group of bacteria includes species with b12-dependent ribonucleotide reductase as well as some with an escherichia coli-type ribonucleotide reductase. | 1979 | 110794 |
| transfer of plasmid-mediated antibiotic resistance from streptococci to lactobacilli. | the transmissible plasmid pambeta1, which codes for erythromycin and lincomycin resistance, was conjugally transferred from a lancefield group f streptococcus to a strain of streptococcus avium. both organisms served as pambeta1 donors for three strains of lactobacillus casei. introduction of pambeta1 into one of the l. casei strains caused the organism to lose its native 6.7 x 10(6)-dalton plasmid. loss of the native plasmid produced no alterations in the organism's growth characteristics or fe ... | 1979 | 104973 |
| synthesis of 5-selenium-substituted uracil derivatives. inhibition of thymidylate synthetase by 5-hydroseleno-2'-deoxyuridylate. | 5-selenium-substituted derivatives (diselenides) or uracil, 2'-deoxyuridine, and 2'-deoxyuridylic acid were synthesized via the addition of methyl hypobromite to the 5,6 double bond, followed by reaction of the adducts with sodium diselenide. the physical and chemical properties of these compounds (including their facile reduction by dithiothreitol and rapid reoxidation) were similar to those of the corresponding 5-sulfur analogues. 5-hydroseleno-2'-deoxyuridylic acid was as potent as 5-mercapto ... | 1979 | 110931 |
| the primary structure of lactobacillus casei thymidylate synthetase. i. the isolation of cyanogen bromide peptides 1 through 5 and the complete amino acid sequence of cnbr 1, 2, 3, and 5. | thymidylate synthetase from lactobacillus casei was s-carboxymethylated and degraded by treatment with cyanogen bromide. although the protein contains 6 methionine residues, only 5 cyanogen bromide peptides were obtained due to the presence of 1 methionine on the nh2 terminus and another adjacent to a threonine residue which was resistant to cleavage. the peptides were isolated by differential extraction, first with ammonium acetate, then pyridine acetate, and finally the residue was solubilized ... | 1979 | 105003 |
| the primary structure of lactobacillus casei thymidylate synthetase. ii. the complete amino acid sequence of the active site peptide, cnbr 4. | the 102 amino acid residues of cnbr 4, the largest of 5 cyanogen bromide peptides from the lactobacillus casei thymidylate synthetase were completely sequenced by means of limited tryptic, tryptic, chymotryptic, and staphylococcal protease peptides. cnbr 4 contains both of the cysteines in an enzyme subunit, with the 5-fluorodeoxyuridylate-reactive cysteine at residue 198 and the other at residue 244. | 1979 | 105004 |
| n-benzoyl derivatives of amino acids and amino acid analogs as inhibitors in microbial antitumor screen. | | 1979 | 106107 |
| enzyme affinity of the 5,6-dihydro derivatives of the substrate and product of thymidylate synthetase catalysis. | the 5,6-dihydro derivatives of 2'-deoxyuridine 5'-phosphate (2) and 2'-deoxythymidine 5'-phosphate (3) were synthesized and characterized. the affinities of 2 and 3 were compared to those of the substrate (2'-deoxyuridine 5'-phosphate) and product (2'-deoxythymidine 5'-phosphate) of the reaction catalyzed by thymidylate synthetase. in both cases, the enzyme affinity of the 5,6-dihydro derivatives was 50 times less than that of the substrate or product. the conclusions from this study are that a ... | 1979 | 106122 |
| deoxyribonucleoside-requiring mutants of bacillus subtilis. | a number of deoxyribonucleoside-requiring mutants (dns) of bacillus subtilis were isolated and their growth characteristics and ribonucleotide reductase activities were compared with those of the wild type and of a dna mutant (tsa13). both tsa13 and dns mutants required the presence of a mixture of deoxyribonucleosides for growth at 45 degrees c but not at 25 degrees c. all the mutant strains tested contained ribonucleotide reductase activity which showed heat sensitivity similar to that of the ... | 1978 | 103994 |
| [clinical value of folic acid determination by radioassay]. | | 1978 | 100876 |
| nuclear magnetic resonance studies of the binding of trimethoprim to dihydrofolate reductase. | the resonances of the aromatic protons of trimethoprim [2,4-diamino-5-(3',4',5'-trimethoxybenzyl)pyrimidine] in its complexes with dihydrofolate reductases from lactobacillus casei and escherichia coli cannot be directly observed. their chemical shifts have been determined by transfer of saturation experiments and by difference spectroscopy using [2',6'-2h2]trimethoprim. the complex of 2,4-diamino-5-(3',4'-dimethoxy-5'-bromobenzyl)pyrimidine with the l. casei enzyme has also been examined. at ro ... | 1979 | 114205 |
| measurement of serum folate by 75se-selenofolate radioassay. results of a multi-institutional clinical trial. | the authors report the multi-institutional evaluation of a radioassay for serum folate utilizing a gamma-labeled folate analog (75se-pteroyl-l-methylselenocysteine). the assay is run as a one-step simultaneous addition at ambient temperature and is completed within 45 minutes. the assay data were reproducible within four study centers and correlated with both the lactobacillus casei diagnostic groups and the clinical pictures. | 1978 | 101078 |
| adenosine triphosphate content in lactobacillus casei and the blender-resistant phage-cell complex-forming ability of cells on infection with pl-1 phage. | the intracellular atp content of lactobacillus casei atcc 27092 grown in a glucose-containing medium was almost constant (2 to 3 microgram/mg dry wt. cells) through the early to middle stage of logarithmic phase, but it was lowered to less than 0.1 microgram/mg after cessation of growth owing to the exhaustion of available glucose. all the cells in the early stage of stationary phase were still viable and thus considered to be in a starved state. when such starved cells were infected with pl-1 p ... | 1979 | 104000 |
| [results of measuring the modulus of elasticity of microorganism cell walls]. | the elasticity modulus of escherichia coli cell walls was found to change in the course of growth of the culture. it decreased in the middle of the exponential phase, apparently as a result of intensification of biosynthetic and related to them transport processes at this period. if the cells were treated with edta, their elasticity modulus was lower, by an order of magnitude, than that of the control cell walls. if the cells were treated with lysozyme, the elasticity modulus of their walls decr ... | 1979 | 106226 |
| influence of neomycin and metronidazole on colonic microflora of volunteers. | the influence on colonic microflora of neomycin and metronidazole alone, or in combination, was studied in volunteers taking a normal diet. bacterial counts on daily faecal samples revealed that metronidazole had no influence on anaerobic faecal bacteria. neomycin on the other hand, was effective against sensitive aerobes. however, the combination of neomycin and metronidazole profoundly reduced both aerobic and bacteria. assay of faecal antibiotic concentrations showed that neomycin achieved hi ... | 1979 | 115830 |
| coupling of energy to folate transport in lactobacillus casei. | lactobacillus casei cells can accumulate folate to an intracellular concentration in excess of 500 mum and to concentration gradients (relative to the extracellular compartment) of several thousand-fold. maximum rates of folate transport are achieved rapidly (t(1/2) < 1 min) after the addition of glucose to energy-depleted cells and occur at intracellular adenosine 5'-triphosphate concentrations above 625 mum. the rate of folate transport and the adenosine 5'-triphosphate content of cells are bo ... | 1979 | 110791 |
| 19f nmr of the 5-fluorodeoxyuridylate-thymidylate synthetase binary complex. | | 1978 | 100115 |
| effect of 5, 10-methylenetetrahydrofolate on the dissociation of 5-fluoro-2'-deoxyuridylate from thymidylate synthetase: evidence for an ordered mechanism. | | 1978 | 101234 |
| the use of triazine inhibitors in mapping the active site region of lactobacillus casei dihydrofolate reductase. | | 1979 | 109047 |
| 5 (alpha-bromoacetyl)-2'=deoxyuridine 5'-phosphate: a mechanism based affinity label for thymidylate synthetase. | | 1979 | 109088 |
| effect of n-trifluoroacetyl derivatives of amino acids and amino acid analogs on microbial antitumor screen. | eighteen trifluoroacetyl derivatives of amino acids and of amino acid analogs were prepared and tested for growth-inhibitory activity using a lactobacillus casei system as a prescreen for antitumor activity. of the compounds tested, the trifluoroacetyl derivatives of o-, m-, and p-fluorophenylalanine and of beta-3-thienylalanine showed modest activity; trifluoroacetyl derivatives of phenylalanine and of beta-2-thienylalanine showed marginal activity. the activity exhibited by the active trifluor ... | 1979 | 108385 |
| biochemical determinants of tumor sensitivity to 5-fluorouracil: ultrasensitive methods for the determination of 5-fluoro-2'-deoxyuridylate, 2'-deoxyuridylate, and thymidylate synthetase. | techniques have been developed to measure fdump, the active metabolite of 5-fura; thymidylate synthetase (tmp synthase; 5,10-methylenetetrahydrofolate:dump c-methyltransferase, ec 2.1.1.45), the target enzyme for this antimetabolite; and dump, the substrate that competes with fdump for binding to tmp synthetase. as little as 0.02 pmol of fdump can be quantitated with a competitive ligand binding assay by using homogeneous lactobacillus casei/mtx tmp synthetase as a binding protein. a new binding ... | 1979 | 108681 |
| intestinal microflora: elimination of germfree characteristics by components of the normal microbial flora. | | 1979 | 117972 |
| interaction of dihydrofolate reductase from amethopterin-resistant lactobacillus casei with cibacron blue, blue dextran, and affi-gel blue. | | 1979 | 109449 |
| folate analogues altered in the c9-n10 bridge region: 11-thiohomofolic acid. | the synthesis of 11-thiohomofolic acid (2) has been accomplished by an unambiguous procedure. reaction of 1-chloro-4-[p-(carbomethoxy)thiophenoxy]-2-butanone (10) with hydroxylamine under carefully controlled conditions gave the corresponding oxime 33. conversion of this oxime to 1-phthalimido-4-[p-(carbomethoxy)thiophenoxy]-2-butanone oxime (4) was carried out by its reaction with potassium phthalimide using crown 18 ether as a catalyst. hydrazinolysis of compound 4 gave 1-amino-4-[p-(carbometh ... | 1979 | 109615 |
| methotrexate analogues. 12. synthesis and biological properties of some aza homologues. | methotrexate analogues, in which an additional nitrogen atom is inserted between the phenyl ring and the carbonyl group of the side chain, were prepared by photochemical methods. the compounds were less inhibitory toward dihydrofolate reductase and thymidylate synthetase derived from lactobacillus casei than was methotrexate. they were also less cytotoxic against human lymphoblastic leukemia cells (ccrf-cem). in vivo against l-1210 leukemia in mice, the aza homologue of methotrexate showed signi ... | 1979 | 109616 |
| synthesis of aza homologues of folic acid. | folic acid analogues containing an additional nitrogen atom between the phenyl ring and the carbonyl group of the side chain were synthesized. none of the compounds showed significant inhibitory activity against human lymphoblastic leukemia cells (ccrf-cem) in culture or against lactobacillus casei (atcc 7469) growth. against l1210 leukemia in mice, the aza homologue of folic acid, 4, and the aspartic acid analogue, 14, showed no increase in life span over control animals. these compounds were m ... | 1979 | 109617 |
| tissue folacin stores in rats measured by radioassay. | | 1979 | 109843 |
| lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes. | the respiratory quinones of 73 strains of gram-positive bacteria including spore-forming rods, lactic-acid bacteria and actinomyctes were examined. menaquinones with seven isoprenoid units (mk-7) were the main quinone type found in representatives of the genus bacillus and in sporolactobacillus inulinus. however, a strain of b. thuringiensis produced mk-8 in addition to mk-7, and strains of b. lentus and b. pantothenticus appeared to produce mk-9 and mk-8, respectively, with no mk-7. in the clos ... | 1979 | 119033 |
| the role of liver passage for conversion of pteroylmonoglutamate and pteroyltriglutamate to active folate coenzyme. | in an attempt to clarify the possible site of conversion of folates to active methylated coenzyme forms mono- and triglutamate was injected rapidly into the portal vein of dogs. samples of blood were removed at various intervals from catheters placed in the portal vein, the hepatic vein and the femoral artery for assay carried out both with l. casei, s. faecalis and liquid scintillating counting. unreduced monoglutamates were extracted in the liver, immediately converted to methyltetrahydrofolat ... | 1979 | 118944 |
| multivariate analysis and quantitative structure-activity relationships. inhibition of dihydrofolate reductase and thymidylate synthetase by quinazolines. | quantitative structure-activity relationships (qsar) have been established for the inhibition of dihydrofolate reductase and thymidylate synthetase by 2,4-diaminoquinazoline-glutamic acid analogues. for dihydrofolate reductase from both human acute lymphocytic leukemia cells and murine l1210r cells, qsar's obtained with 50 quinazolines were similar. on the other hand, for the inhibition of thymidylate synthetase from murine l1210s cells and from lactobacillus casei, qsar's formulated on the basi ... | 1979 | 110930 |
| the presence of antibody-coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy. | quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. the fluorescent antibody (fa) test was applied to the sediments of bladder aspirates to determine the site of infection. anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were fa-positive. these anaerobes wer ... | 1979 | 118996 |
| immunological cross-reactivity of oral non-streptococcal bacteria with mammalian tissue. | the presence of cross-reacting antigens between oral non-steptococcal bacteria and human and monkey tissue was investigated by indirect immunofluorescence. rabbit antisera, prepared against 22 oral bacteria, were incubated with cryostatcut sections of heart, skeletal muscle, brain cord, liver, kidney, and skin. a cell wall antigen of lactobacillus casei was shared with neuroglial brain cells, and an antigenic component of propionibacterium acnes was shared with kidney glomeruli. tissue-reactive ... | 1979 | 110702 |
| [bacteriological examination of amniotic fluid after intra-amnial tocometry sub partu (author's transl)]. | two sets of group of 50 women were examined and compared to find out if the introduction of continuous intra-uterine catheter during labour increased infection. amniotic fluid was withdrawn from both groups and examined. also a catheter was introduced into the amniotic sac of one group and after 2 and 5 hours amniotic fluid was also withdrawn from both groups and examined. inhaled amniotic fluid from the infant was also withdrawn and bacteriologically examined both quantitatively and qualitative ... | 1979 | 121183 |
| prophylaxis against ampicillin-associated diarrhea with a lactobacillus preparation. | the efficacy of a lactobacillus preparation (lactinex) in preventing ampicillin-associated diarrhea in adult inpatients was studied. in a double-blind procedure, 98 patients were assigned randomly to receive one packet of lactinex (lactobacillus acidophilus and l bulgaricus) or placebo four times daily for the first five days of ampicillin therapy. cases of diarrhea were reviewed independently and classified according to etiology. complete data were obtained for 79 patients--43 received placebo ... | 1979 | 111546 |
| thymidylate synthetase catalyzed exchange of tritiumfrom [5-3h]-2'-deoxyuridylate for protons of water. | thymidylate synthetase catalyzes an exchange of tritium of [5-3h]dump for protons of water in the absence of ch2-h4folate. the turnover number for this reaction is some 45,000-fold lower than that of dtmp formation and km is 1.2 x 10(-5) m, similar to the dissociation constant of the enzyme-dump complex determined by equilibrium dialysis. the presence of 4 mm folate has no effect on vmax but results in a decrease in the km of dump to a value close to that in the normal enzymic reaction. the exch ... | 1979 | 113030 |
| quinazoline analogues of folic acid as inhibitors of thymidylate synthetase from bacterial and mammalian sources. | | 1979 | 113660 |
| 5-fluoro-2'deoxyuridine 5'-(p-azidophenyl phosphate), a potential photoaffinity label of thymidylate synthetase. | 5-fluoro-2'-deoxyuridine 5'-(p-azidophenyl phosphate) (1), a potential photoaffinity labeling reagent for thymidylate synthetase from a methotrexate-resistant strain of lactobacillus casei, has been synthesized and characterized. uv254 irradiation of mixtures of thymidylate synthetase with 1, containing 14c-labeled phenyl and 3h-labeled pyrimidine rings, in the presence of excess 5,10-methylenetetrahydrofolate, the cofactor for the reaction, produced two complexes, separable from the native enzy ... | 1979 | 114656 |
| 5-[(4-methyl-1,2,3,4-tetrahydroquinoxalyl)methyl]-2'-deoxyuridine 5'-phosphate: an analogue of a proposed intermediate in thymidylate synthetase catalysis. | in a study of the sequence steps involved in the mechanism of thymidylate synthetase catalysis, 5-[(n-methyl-piperazinyl)methyl]- (5) and 5-[(4-methyl-1,2,3,4-tetrahydroquinoxalyl)methyl]-2'-deoxyuridine 5'-phosphate (6) were synthesized. compound 6 has high affinity for the lactobacillus casei enzyme (ki = 0.75 microm, ki/km - 0.23), which is 50 times stronger than that of the piperazinyl derivative 5. compound 6, a possible multisubstrate inhibitor, is an analogue of a proposed intermediate in ... | 1979 | 114659 |
| 5-cyano-2'-deoxyuridine 5'-phosphate: a potent competitive inhibitor of thymidylate synthetase. | the 5'-phosphate (1) of the antiviral nucleoside 5-cyano-2'-deoxyuridine was synthesized and evaluated for inhibition of thymidylate synthetase purified from methotrexate-resistant lactobacillus casei. compound 1 was a potent competitive inhibitor with a k1 of 0.55 microns. irreversible enzyme inhibition by this compound could not be detected. | 1979 | 114660 |
| drug therapy reviews: pharmacotherapy of diarrhea. | gastrointestinal physiology, and the pathophysiology, diagnosis, symptoms and treatment of acute and chronic diarrhea are reviewed. drugs used in the treatment of diarrhea include opiates (morphine, codeine), synthetic anti-diarrheals (diphenoxylate, loperamide), anticholinergics (atropine, propantheline), adsorbents (kaolin, pectin, cholestyramine resin) and lactobacillus acidophilus. chronic diarrhea and acute diarrhea caused by microorgansims, drugs and viruses are described. the management o ... | 1979 | 111547 |
| [experience with enzyme- and lactic-acid-bacteria-containing preparations (acidofer and acidofer-forte; ctb chemotherapia, bielefeld) in the prevention and therapy of calf diarrhea]. | | 1979 | 111668 |
| the interaction of ligands with enzymes. a starting point in drug design. | the problem of inhibiting a specific enzyme of a pathogen without inhibiting the corresponding enzyme of the host is one which has intrigued medicinal chemists for years. while it is easy to find potent inhibitors of purified enzymes transforming these inhibitors into effective drugs, it still is a very poorly understood business. this report discusses studies with two enzymes, papain and dihydrofolate reductase, and the nonspecific (phi and mr correlated) interaction of ligands with them. the q ... | 1979 | 111968 |
| evidence for a sequential interaction of the subunits of thymidylate synthetase. | | 1979 | 108277 |
| n-benzoyl derivatives of amino acids and amino acid analogs as growth inhibitors in microbial antitumor screen. | twenty-seven n-benzoyl derivatives of amino acids and amino acid analogs were prepared and tested for growth-inhibitory activity in a microbial antitumor screen. of these, 19 showed some inhibitory capacity, from a modest 13% to a potent 96% at 1 mg/ml. the activities of the "modest" inhibitors were comparable to those of most inhibitory chloracetyl and trifluoroacetyl derivatives reported earlier. the intermediate inhibitors were as active as n-chloroacetyl-beta-hydroxy-d-norleucine isomer b, t ... | 1979 | 117094 |
| bacterial and strain specificities in opsonization, phagocytosis and killing of streptococcus mutans. | opsonization of streptococcus mutans, followed by phagocytosis and killing by polymorphonuclear leucocytes has been postulated as an effector mechanism in protection against dental caries. opsonization was studied by using sera from monkeys immunized with killed strep. mutans (sero-type c) and compared with sera from sham-immunized monkeys. antibodies to strep. mutans (sero-type c) induced maximal phagocytosis and killing of serotypes c and e, and this was significantly greater than with serotyp ... | 1979 | 106996 |
| interpretation of serum and red cell folate results. a comparison of microbiological and radioisotopic methods. | serum folate and red blood cell folate levels were measured in 167 patients using a microbiological method (lactobacillus casei) and a commercial 125i radioassay kit. the results of the two methods were compared and related to the clinical findings to see which method more truly reflected the physiological folate status of the patient. there was a good linear correlation between the methods for both serum folate and red cell folate over the whole range encountered and the mean results obtained w ... | 1979 | 107505 |
| translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model. | viable bacteria were not cultured from the mesenteric lymph nodes, spleens, or livers of specific-pathogen-free (spf) mice. viable enteric bacteria, primarily indigenous escherichia coli and lactobacilli, were present in the mesenteric lymph nodes of gnotobiotic mice inoculated intragastrically with the whole cecal microflora from spf mice but not in the nodes of control spf mice similarly inoculated. these indigenous e. coli also were cultured from the mesenteric lymph nodes of 96% of gnotobiot ... | 1979 | 154474 |
| susceptibility to adjuvant-induced arthritis among germfree, specific-pathogen-free, and conventional rats. | germfree f344 rats developed severe arthritis with 100% incidence after a single intradermal inection of either squalane containing 0.5 mg of heat-killed mycobacterium bovis bcg or a water-in-oil emulsion containing 0.2 mg of peptidoglycan derived from staphylococcus epidermidis. conventional f344 rats developed less-severe arthritis with 20% incidence for heat-killed bcg and 0% incidence for peptidoglycan. specific-pathogen-free rats showed an intermediate susceptibility between germfree and co ... | 1979 | 160888 |
| inhibition of dihydrofolate reductases by derivatives of 2,4-diaminopyrroloquinazoline. | | 1979 | 118761 |
| mechanism of folate transport in lactobacillus casei: evidence for a component shared with the thiamine and biotin transport systems. | lactobacillus casei cells have been shown previously to utilize two separate binding proteins for the transport of folate and thiamine. folate transport, however, was found to be strongly inhibited by thiamine in spite of the fact that the folate-binding protein has no measurable affinity for thiamine. this inhibition, which did not fluctuate with intracellular adenosine triphosphate levels, occurred only in cells containing functional transport systems for both vitamins and was noncompetitive w ... | 1979 | 108244 |
| essential arginyl residues in thymidylate synthetase from amethopterin-resistant lactobacillus casei. | | 1979 | 117827 |
| pentitol metabolism in lactobacillus casei. | strains of lactobacillus casei capable of growing on either ribitol or xylitol carry out a heterolactic fermentation producing ethanol, acetate, and a mixture of d- and l-lactate. following conversion of the pentitols to ribulose 5-phosphate or xylulose 5-phosphate via enzymatic steps unique to these organisms, the intermediate products are further metabolized by enzymes of the pentose pathway. the initial enzymes of the pathway, i.e., pentitol:phosphoenolypyruvate phosphotransferase and penitit ... | 1979 | 118163 |
| 5-(alpha-bromoacetyl)-2'-deoxyuridine 5'-phosphate: an affinity label for thymidylate synthetase. | 5-(alpha-bromoacetyl)-2'-deoxyuridine 5'-phosphate (1) is an active-site-directed irreversible inhibitor of thymidylate synthetase from lactobacillus casei. analysis of the rate of inactivation of the enzyme in the presence of substrate confirmed the intermediate formation of a reversible enzyme-inhibitor complex. | 1979 | 119854 |
| [oxygen consumption of lactobacilli. ii. relationship between nadh oxidase activity and oxygen consumption of lactobacillus acidophilus (author's transl)]. | | 1979 | 120438 |
| a radiometric microbiologic assay for the biologically active forms at niacin. | a radiometric microbiologic assay has been developed for the determination of niacin in biologic fluids. lactobacillus plantarum produced 14co2 from l-[u-14c] hr malic acid in quantities proportional to the amount of niacin present. the assay is specific for the biologically active forms of niacin in humans. thirty normal hemolysates were analyzed and the values ranged from 13.0 to 17.8 micrograms niacin/ml rbc (mean = 15.27 +/- 1.33 s.d.). good recovery and reproducibility studies were obtained ... | 1979 | 120418 |
| vitamin b2 activity of 7,8-dimethyl-10-(2,3,4-trihydroxy-4-formylbutyl)isoalloxazine in lactobacillus casei. | the microbial activities of vitamin b2-aldehyde and vitamin b2-acid, produced by schizophyllum commune, a basidiomycete, were studied. lactobacillus casei atcc no. 7469 was used as a test microorganism. b2-aldehyde exhibited a good response curve in the growth of l. casei. b2-acid had neither a stimulatory nor an inhibitory effect on the growth. when b2-aldehyde was incubated with the homogenate of l. casei, it was converted to riboflavin. the flavin formed from b2-aldehyde by the homogenate not ... | 1979 | 120425 |
| quenching of thymidylate synthetase fluorescence by substrate analogs. | | 1975 | 125086 |
| [the use of antibiotic-resistant bifidobacterium biphidum and lactobacillus acidophilus in the course of antibiotic therapy]. | | 1979 | 119605 |
| [microbiological characteristics in rats given cariogenic diet following the application of fluoride containing toothpastes]. | the paper deals with experimental research work carried out on wistar rats, regarding the effect of fluoride tooth-pastes in varying compounds on microflora in the oral cavity. the findings show that the most favourable effect on the microbial situation in the cavum oris is exerted by fluoride tooth-pastes prepared on a calcium-free basis. | 1975 | 126614 |
| molecular aspects of the mechanism of action of 5-fluorodeoxyuridine. | | 1975 | 127539 |
| some data on establishing a specific-pathogen-free guinea pig colony. | detailed procedures for the germfree delivery of guinea pigs using a "dry hysterectomy" technic were given; 320 animals were alive after hysterectomy, but there was a subsequent loss of 37% of the animals, mainly during the first and second weeks. their specific-pathogen-free maintenance, feeding, and association with an anerobic flora were described. feeding 3 times a day vs twice a day decreased mortality from 48% to 20%. | 1976 | 131877 |
| variations in melanin and riboflavin content of amphibian liver under the influence of reserpine and amphetamine. | by means of paper chromatography, lactobacillus casei test and the staining method with the feulgen reaction, the effects of reserpine and amphetamine on riboflavin and melanin were studied in the liver of triturus cristatus. it was demonstrated that the concentrations of both melanin and riboflavin can be altered by the influence of these drugs. from the results obtained it is suggested that riboflavin shows a correlation with the density of melanin. | 1976 | 132047 |
| denaturation of thymidylate synthetase from amethopterin-resistant lactobacillus casei. | the effects of various concentrations of urea and guanidine hydrochloride on enzyme activity and on subunit association were determined. incubation of thymidylate synthetase with buffered solutions of 3m to 3.5m guanidine hydrochloride or 5 m to 6 m urea resulted in the loss of about 90% of the enzyme activity. under these denaturing conditions a red shift of the fluorescence emission maximum from 340 nm to 351 nm was observed together with a significant decrease in the relative fluorescence int ... | 1979 | 121920 |
| antifolate studies. activities of 40 potential antimalarial compounds against sensitive and chlorguanide triazine resistant strains of folate-requiring bacteria and escherichia coli. | as part of the search for new antimalarial drugs, a screening program was developed using sensitive and chlorguanide triazine (cgt, cycloguanil) resistant strains of the folate-requiring bacteria, streptococcus faecium durans, lactobacillus casei, and pediococcus cerevisiae. the activities of 40 compounds have been studied against these strains and escherichia coli. observations have been made on the points of 50% growth inhibition, the fold increase of resistance shown to each compound by the r ... | 1977 | 137981 |
| characterization of the glycolysis pathway and the plasma membrane of lactobacillus and bifidobacterium strains. | the characteristics of the glycolytic pathway and the plasma membrane of lactobacillus and bifidobacterium were studied. the enzyme system of glycolysis (hexokinase, glucokinase and pyruvate kinase) which is the main source of energy in the anaerobic condition was localized in the cell soluble fraction (cytoplasma) of all species examined. neither electron transfer chain components nor oxidase activities were found in anaerobically cultured lactobacillus and bifidobacterium. adenosine triphospha ... | 1976 | 138331 |
| thymidylate synthetase and 2'-deoxyuridylate form a tight complex in the presence of pteroyltriglutamate. | thymidylate synthetases of human and bacterial origin form a tightly bound complex with the substrate dump in the presence of pteroyltriglutamate. this complex and the weaker enzyme . dump binary complex can be isolated and conveniently assayed by nitrocellulose disc filtration using [6-3h]dump as the radioactive ligand. intact thymidylate synthetase . dump . pteroyltriglutamate complex can be obtained by gel filtration chromatography on sephadex g-25, but the binary enzyme . dump complex dissoc ... | 1979 | 115883 |
| polyneuropathy and folate deficiency. | we studied five patients (two men and three women, age between 58 and 76 years) with clinical and electrophysiological signs of polyneuropathy. routine neurological, hematological, and gastroenterological studies as well as procedures to test fat malabsorption were performed. folate determinations were done using both radioactive and lactobacillus casei methods. two patients displayed the signs of subacute combined degeneration of the spinal cord with polyneuropathy, while three had only signs o ... | 1978 | 210747 |
| deoxynucleoside kinases from lactobacillus acidophilus r-26. | | 1978 | 211384 |
| participation of exogenous thymine and thymidine in deoxyribonucleic acid synthesis in lactobacillus acidophilus. | the degree of participation (dp) of exogenous thymidine and thymine in overall dna synthesis was studied in lactobacillus acidophilus r-26. the dp of thymidine remains constant under a variety of conditions (except at low thymidine concentrations, when the dp is influenced by deoxyribonucleosides and folic acid). a 5-bromodeoxyuridine-resistant mutant was isolated, which displayed cross-resistance to 5-bromouracil and a significantly lower dp of thymidine than the parental strain. thymine was po ... | 1975 | 123477 |
| synergistic lysis of erythrocytes by propionibacterium acnes. | sheep and human erythrocytes, partially processed by staphylococcus aureus or clostridium perfringens, were susceptible to lysis in the presence of propionibacterium acnes. p. acnes liberated a lipase that was detected on tween 80 agar and also on phospholipase c-precipitated egg yolk agar. such a lipase might have contributed in the process of an intensified cellular lysis. similar reactions were attempted with lactobacillus acidophilus, known to possess a nondiffusible lipase, and failed to pr ... | 1978 | 212450 |
| [the behavior of oral microorganisms after application of fluoride containing gels]. | | 1975 | 125992 |
| [the microbial population of the gingival sulcus of mongoloid and normal children--a comparative study]. | | 1976 | 145388 |
| reaction mechanism of non-allosteric phosphofructokinase. | the reaction mechanism of the non-allosteric phosphofructokinase from lactobacillus plantarum was investigated by initial-rate bisubstrate kinetics and product inhibition kinetics adn by the measurement of equilibrium isotope exchange in the presence of various substrate and product concentrations. the reaction mechanism is clearly sequential. the product inhibition and equilibrium isotope-exchange patterns are consistent with an ordered bi-bi reaction sequence with fructose 6-phosphate as the l ... | 1978 | 149659 |
| models for study of the specificity by which indigenous lactobacilli adhere to murine gastric epithelia. | a lactobacillus strain isolated from a mouse (indigenous) and another strain isolated from swine feces (nonindigenous) were compared in two model systems for their ability to adhere in vitro and in vivo to keratinizing squamous and columnar epithelia of mouse stomachs. in one model, stomachs dissected from specific-pathogen-free or germfree mice were injected with suspensions of lactobacilli labeled with [(3)h]thymidine and incubated at 37 degrees c. thereafter, the non-secreting and secreting t ... | 1979 | 160893 |
| [an attempt at plaque formation and caries induction in rodents by oral administration of extracellular glucosyltransferase from streptococcus mutans]. | | 1979 | 161982 |